Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0003396

Physical Location & Seq

Genomic Coordinates Reverse (-)

5384726 .. 5389304

Gene Structure 4579 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1066700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2067 bp

ATGAAAGGGGAGGGGAAGATGGTCTTGAGGTCAAAGATGAAATGGGTTGGACTGGTTGGTCTGTTTCTTTCAGCTATCTCTCTCTTTGTGCACTTTCTGCTGGCCAGATTCACAGAAGGGGCTGCTGCTTCAGAGTTCCACTCCTCCATCACAATCTTCCCCAGAAGAGCCATTGGTGAAAGCTTGAATTTTTCCAAGACTAGTCCAATGTATAGAAGGCTATGGGGTCCGGTTCGGCATCTCGAATCTTTGCAGCCGAATGCAAATCCCAGAGGACATTATGCCGAACCCGATTCAGATACCAGCAGGTTTATCTTTGTCAGGATACAAGGCGGATTTCATGAGATCAGGAATTCGATATGTGATGTAGTTGTGGTTTCTCGGTTTCTTAATGCAACGTTAGTGATTCCTGAGATCCAATCAACCACAAGCAGCAAGGGCATAAGTTCTCAGTTTAAAAGTTTTGCCTATCTCTACAATGAGGACCAATTCATGGCAGCCTTGTCAAAAGACGTCAAAGTTGTGAAGACGCTTCCCAATAATCTTAAAGGGCAAAGGAGGAAAAAGAACATTCCGGTGTTTAAAGTGCCTTACTCTGCTTCACCATATTACTACAAGAACAATGTTCTTCCAATTTTGAAGAAGCATTTAGTAGTTGAACTAGTTGTGTCTGAAGGTGGATGCTTGCAGGCTATCCTGCCACCTGATCTTGAAGAGTATCAGAGGCTGAGATGTAGGGTTGCTTTTCACGCCCTTAGGTTCCGGCAGGAGGTCCAGGAACTTGCCACCGAAATTTTACAAAGATTAAAGGCTCCTGGACAGCCATTCATAGCATTTGACCCTGGCATGACCCGGGAGACTTTGGCATATCATGGTTGTGCTGAACTCTTCCAGGATGTACACACGGAACTCATTCAACACAAAAGGCAATGGATGAGAAAACGTGGAATTGTCAAGGGAAAGCTTTCGGTGAATTCTGCAAAACAACGCCTTAAAGGTTCTTGCCCCCTAATGCCAGAAGAGATTGGTATTATTCTTCGTGCATATGGGTACTTGTGGGACACAATAATATATGTTTCGGGGGGAGAAATCTTTGGTGGCCAAAGGACGTTGATCCCTCTTCGTGCAATGTTTGAAAATGTCGTTGATAGGACTTCCCTCAGCATGCCTTGGGAGCTTAGTAGGATGTATGGTCGTGAAGCTAACCTAGTCGATCGCAATCCCGGGAGTCCACCAATGATTGAGGAAGAGATGAAAATTGAAGCATGGAAAAATGCAGGTCCTCGTCCTCGCCCACTTCCACCACCCCCGGCCAGGCCCAAATCCTATAACATTGAAGGTTGGTGGGGTTGGGTAGCGGAGAGTGATAACGAGCCTGATAGTACAGTTATTGAATTGAGGACCAATGCTCATAAACTTCTGTGGGAAGCAATTGATTATGTGATATGCGTTGAAGCTGATGTGTTTATCCCTGGATTTGATTTTGATGGTAAGGGACACCCAAATTTTGCGAGCTTGGTAATGGGACAGCGACTCTATCAATCAGCTGATTCAAAGACATACAGACCAGACAGAAAAGAAGTCGCCAAGCTTTTAGATGAAATTCATGACCACCTATACCACGCAAACCATACTTGGCTGATGTCAGTACGCAAGCATTTGAGAAAGAGATTAGTTGATGGATTAATAGAAGCTTCCGCAAGATCAAAGCCGCTGTCTTTTATTTCTCATCCAATCCCGGAATGTTCTTGCTTAAGGCAAGATCCTGCTGGAAAATCAATCAATGCCACGTCATCACCTCATGCTTCAACTCCTAGTCATTCTCAAGTTCTTTCTGCTCTTGGAGTCGTGCACTATTGCCCTGCTTGGATGGAGAATGACTTGACATTGCAGTCGAAATCAAAAGACAAGGAAAATGAAGAAACTCTAGATGAAGACGACTCCACCTCATCGGGGTTGTTCTTCCGTAAAAATAATGGAGATCATGATGGTGGTAATGGAGACATAAGCAACAAAGAAGAAACTCAGTTGGAGGATCAAGAAGAGCTCGAGGGTGGAGAAAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0012505 GO:0031410 GO:0031982 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

O-FucT

Protein Analysis

689

Amino Acids

77.92

Weight (kDa)

8.37

Isoelectric Point (pI)

54.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
O-FucT	PF10250	104 - 405	2.3e-67	GDP-fucose protein O-fucosyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003396)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G30300 AT3G30300
fragaria_vesca	FvH4_4g06281 FvH4_6g46210 FvH4_6g46210 FvH4_6g46210
malus_domestica	MD09G1076000.v1.1 MD17G1066700.v1.1
prunus_persica	Prupe.3G248000_v2.0.a1
pyrus_communis	pycom09g00340
rosa_chinensis	RchiOBHm_Chr2g0164661 RchiOBHm_Chr6g0258841 RchiOBHm_Chr6g0258851
rosa_laevigata	RLG00000014579 RLG00000021475
rosa_roxburghii	Rroxscaffold_2G00086400 Rroxscaffold_3G00256120 Rroxscaffold_3G00256130 Rroxscaffold_5G00351080 Rroxscaffold_7G00206110
rosa_rugosa	Rorug02G0509600 Rorug05G0583900.1
rosa_samantha	Rh1AG019300 Rh1CG016900 Rh1DG013900 Rh2AG577500 Rh2BG588700 Rh2CG559500 Rh2DG599300 Rh6AG100300 Rh6CG089300 Rh6DG083600 Rh6DG083700
rosa_wichuraiana	Rw1G001260 Rw2G047960 Rw6G008690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	57, 1891
AatII	GACGTC	1 cut(s)	516
Acc36I	ACCTGC	2 cut(s)	297, 1268
AccB7I	CCANNNNNTGG	1 cut(s)	493
AciI	CCGC	4 cut(s)	333, 1358, 1698, 1712
AclI	AACGTT	1 cut(s)	398
AclWI	GGATC	4 cut(s)	409, 1108, 1757, 2043
AcoI	YGGCCR	3 cut(s)	102, 1099, 1311
AcsI	RAATTY	6 cut(s)	187, 352, 792, 973, 1504, 1602
AcuI	CTGAAG	2 cut(s)	114, 693
AcyI	GRCGYC	1 cut(s)	513
AfaI	GTAC	4 cut(s)	900, 1052, 1384, 1650
AfiI	CCNNNNNNNGG	4 cut(s)	493, 769, 1314, 1953
AflII	CTTAAG	1 cut(s)	1753
AhlI	ACTAGT	2 cut(s)	200, 661
AjiI	CACGTC	1 cut(s)	1791
AjnI	CCWGG	6 cut(s)	774, 814, 841, 891, 1313, 1471
Alw21I	GWGCWC	3 cut(s)	93, 1854, 2049
Alw26I	GTCTC	2 cut(s)	851, 1995
Alw44I	GTGCAC	2 cut(s)	89, 1850
AlwI	GGATC	4 cut(s)	409, 1108, 1757, 2043
AlwNI	CAGNNNCTG	1 cut(s)	727
Ama87I	CYCGRG	3 cut(s)	852, 1223, 2048
AoxI	GGCC	4 cut(s)	102, 1099, 1311, 1316
ApaLI	GTGCAC	2 cut(s)	89, 1850
ApeKI	GCWGC	5 cut(s)	122, 125, 253, 432, 497
ApoI	RAATTY	6 cut(s)	187, 352, 792, 973, 1504, 1602
ArsI	GACNNNNNNTTYG	2 cut(s)	1700, 1732
AseI	ATTAAT	1 cut(s)	1685
Asp700I	GAANNNNTTC	2 cut(s)	912, 964
AspS9I	GGNCC	6 cut(s)	227, 484, 772, 1280, 1317, 1401
AsuC2I	CCSGG	6 cut(s)	853, 854, 1224, 1225, 1310, 1739
AsuHPI	GGTGA	4 cut(s)	188, 594, 982, 1788
AvaI	CYCGRG	3 cut(s)	852, 1223, 2048
AvaII	GGWCC	5 cut(s)	227, 484, 772, 1280, 1401
AxyI	CCTNAGG	1 cut(s)	755
BaeGI	GKGCMC	2 cut(s)	93, 1854
BalI	TGGCCA	2 cut(s)	104, 1101
BanII	GRGCYC	1 cut(s)	2049
BbsI	GAAGAC	2 cut(s)	533, 1941
Bbv12I	GWGCWC	3 cut(s)	93, 1854, 2049
BbvCI	CCTCAGC	1 cut(s)	1160
BbvI	GCAGC	5 cut(s)	109, 112, 265, 444, 509
BccI	CCATC	6 cut(s)	13, 155, 1481, 1673, 1864, 1982
BciT130I	CCWGG	6 cut(s)	776, 816, 843, 893, 1315, 1473
BciVI	GTATCC	1 cut(s)	318
BcnI	CCSGG	6 cut(s)	853, 854, 1224, 1225, 1310, 1739
BcoDI	GTCTC	2 cut(s)	851, 1995
BcuI	ACTAGT	2 cut(s)	200, 661
BfaI	CTAG	5 cut(s)	201, 662, 1208, 1815, 1928
BfrI	CTTAAG	1 cut(s)	1753
BfuAI	ACCTGC	2 cut(s)	297, 1268
BfuI	GTATCC	1 cut(s)	318
BisI	GCNGC	6 cut(s)	123, 126, 254, 433, 498, 1712
BlsI	GCNGC	6 cut(s)	124, 127, 255, 434, 499, 1713
Bme18I	GGWCC	5 cut(s)	227, 484, 772, 1280, 1401
BmeT110I	CYCGRG	3 cut(s)	852, 1223, 2048
BmgBI	CACGTC	1 cut(s)	1791
BmgT120I	GGNCC	6 cut(s)	227, 484, 772, 1280, 1317, 1401
BmiI	GGNNCC	3 cut(s)	228, 761, 813
BmsI	GCATC	2 cut(s)	247, 671
BpiI	GAAGAC	2 cut(s)	533, 1941
BplI	GAGNNNNNCTC	2 cut(s)	125, 157
Bpu10I	CCTNAGC	1 cut(s)	1160
BpuEI	CTTGAG	2 cut(s)	46, 1809
BpuMI	CCSGG	6 cut(s)	853, 854, 1224, 1225, 1310, 1739
BsaBI	GATNNNNATC	1 cut(s)	1218
BsaHI	GRCGYC	1 cut(s)	513
BsaJI	CCNNGG	6 cut(s)	841, 852, 1169, 1223, 1308, 1471
BsaWI	WCCGGW	2 cut(s)	229, 574
Bsc4I	CCNNNNNNNGG	4 cut(s)	493, 769, 1314, 1953
Bse1I	ACTGG	1 cut(s)	57
Bse21I	CCTNAGG	1 cut(s)	755
Bse3DI	GCAATG	3 cut(s)	935, 1134, 1886
Bse8I	GATNNNNATC	1 cut(s)	1218
BseBI	CCWGG	6 cut(s)	776, 816, 843, 893, 1315, 1473
BseDI	CCNNGG	6 cut(s)	841, 852, 1169, 1223, 1308, 1471
BseGI	GGATG	6 cut(s)	686, 901, 939, 1191, 1729, 1875
BseJI	GATNNNNATC	1 cut(s)	1218
BseLI	CCNNNNNNNGG	4 cut(s)	493, 769, 1314, 1953
BseMI	GCAATG	3 cut(s)	935, 1134, 1886
BseMII	CTCAG	5 cut(s)	402, 464, 719, 1174, 2039
BseNI	ACTGG	1 cut(s)	57
BseRI	GAGGAG	1 cut(s)	133
BseSI	GKGCMC	2 cut(s)	93, 1854
BseXI	GCAGC	5 cut(s)	109, 112, 265, 444, 509
Bsh1285I	CGRYCG	1 cut(s)	1216
BshFI	GGCC	4 cut(s)	104, 1101, 1313, 1318
BsiEI	CGRYCG	1 cut(s)	1216
BsiHKAI	GWGCWC	3 cut(s)	93, 1854, 2049
BsiHKCI	CYCGRG	3 cut(s)	852, 1223, 2048
BsiSI	CCGG	7 cut(s)	230, 575, 763, 853, 1224, 1310, 1739
BslFI	GGGAC	3 cut(s)	1073, 1509, 1539
BslI	CCNNNNNNNGG	4 cut(s)	493, 769, 1314, 1953
BsmAI	GTCTC	2 cut(s)	851, 1995
BsmFI	GGGAC	3 cut(s)	1073, 1509, 1539
BsmI	GAATGC	1 cut(s)	265
BsnI	GGCC	4 cut(s)	104, 1101, 1313, 1318
BsoBI	CYCGRG	3 cut(s)	852, 1223, 2048
Bsp1286I	GDGCHC	3 cut(s)	93, 1854, 2049
Bsp1407I	TGTACA	1 cut(s)	898
Bsp143I	GATC	9 cut(s)	345, 414, 706, 1113, 1213, 1703, 1762, 1981, 2035
BspACI	CCGC	4 cut(s)	333, 1358, 1698, 1712
BspANI	GGCC	4 cut(s)	104, 1101, 1313, 1318
BspCNI	CTCAG	5 cut(s)	403, 463, 720, 1173, 2038
BspHI	TCATGA	3 cut(s)	340, 1606, 1984
BspLI	GGNNCC	3 cut(s)	228, 761, 813
BspMI	ACCTGC	2 cut(s)	297, 1268
BspPI	GGATC	4 cut(s)	409, 1108, 1757, 2043
BspQI	GCTCTTC	2 cut(s)	160, 2037
BspTI	CTTAAG	1 cut(s)	1753
BsrDI	GCAATG	3 cut(s)	935, 1134, 1886
BsrGI	TGTACA	1 cut(s)	898
BsrI	ACTGG	1 cut(s)	57
BssECI	CCNNGG	6 cut(s)	841, 852, 1169, 1223, 1308, 1471
BssMI	GATC	9 cut(s)	345, 414, 706, 1113, 1213, 1703, 1762, 1981, 2035
BssNI	GRCGYC	1 cut(s)	513
BssT1I	CCWWGG	1 cut(s)	1169
Bst2UI	CCWGG	6 cut(s)	776, 816, 843, 893, 1315, 1473
Bst4CI	ACNGT	1 cut(s)	1387
Bst6I	CTCTTC	7 cut(s)	160, 708, 893, 1014, 1125, 1242, 2037
BstACI	GRCGYC	1 cut(s)	513
BstAFI	CTTAAG	1 cut(s)	1753
BstAPI	GCANNNNNTGC	1 cut(s)	97
BstAUI	TGTACA	1 cut(s)	898
BstC8I	GCNNGC	6 cut(s)	102, 686, 690, 1166, 1513, 1655
BstDEI	CTNAG	7 cut(s)	411, 450, 728, 755, 1160, 1178, 2025
BstF5I	GGATG	6 cut(s)	686, 901, 939, 1191, 1729, 1875
BstKTI	GATC	9 cut(s)	348, 417, 709, 1116, 1216, 1706, 1765, 1984, 2038
BstMAI	GTCTC	2 cut(s)	851, 1995
BstMBI	GATC	9 cut(s)	345, 414, 706, 1113, 1213, 1703, 1762, 1981, 2035
BstMCI	CGRYCG	1 cut(s)	1216
BstMWI	GCNNNNNNNGC	4 cut(s)	97, 438, 749, 1174
BstNI	CCWGG	6 cut(s)	776, 816, 843, 893, 1315, 1473
BstNSI	RCATGY	1 cut(s)	1168
BstSLI	GKGCMC	2 cut(s)	93, 1854
BstV1I	GCAGC	5 cut(s)	109, 112, 265, 444, 509
BstV2I	GAAGAC	2 cut(s)	533, 1941
BstX2I	RGATCY	2 cut(s)	414, 1762
BstYI	RGATCY	2 cut(s)	414, 1762
Bsu36I	CCTNAGG	1 cut(s)	755
BsuI	GTATCC	1 cut(s)	318
BsuRI	GGCC	4 cut(s)	104, 1101, 1313, 1318
BtrI	CACGTC	1 cut(s)	1791
BtsCI	GGATG	6 cut(s)	686, 901, 939, 1191, 1729, 1875
BveI	ACCTGC	2 cut(s)	297, 1268
Cac8I	GCNNGC	6 cut(s)	102, 686, 690, 1166, 1513, 1655
CaiI	CAGNNNCTG	1 cut(s)	727
CciI	TCATGA	3 cut(s)	340, 1606, 1984
Cfr13I	GGNCC	6 cut(s)	227, 484, 772, 1280, 1317, 1401
Cfr9I	CCCGGG	2 cut(s)	852, 1223
CseI	GACGC	1 cut(s)	538
Csp6I	GTAC	4 cut(s)	899, 1051, 1383, 1649
CviAII	CATG	9 cut(s)	341, 493, 847, 872, 1165, 1266, 1607, 1802, 1985
CviQI	GTAC	4 cut(s)	899, 1051, 1383, 1649
DdeI	CTNAG	7 cut(s)	411, 450, 728, 755, 1160, 1178, 2025
DpnI	GATC	9 cut(s)	347, 416, 708, 1115, 1215, 1705, 1764, 1983, 2037
DpnII	GATC	9 cut(s)	345, 414, 706, 1113, 1213, 1703, 1762, 1981, 2035
DraI	TTTAAA	2 cut(s)	457, 583
DrdI	GACNNNNNNGTC	2 cut(s)	57, 1891
DseDI	GACNNNNNNGTC	2 cut(s)	57, 1891
EaeI	YGGCCR	3 cut(s)	102, 1099, 1311
Eam1104I	CTCTTC	7 cut(s)	160, 708, 893, 1014, 1125, 1242, 2037
EarI	CTCTTC	7 cut(s)	160, 708, 893, 1014, 1125, 1242, 2037
EciI	GGCGGA	1 cut(s)	348
Ecl136II	GAGCTC	1 cut(s)	2047
Eco130I	CCWWGG	1 cut(s)	1169
Eco24I	GRGCYC	1 cut(s)	2049
Eco47I	GGWCC	5 cut(s)	227, 484, 772, 1280, 1401
Eco53kI	GAGCTC	1 cut(s)	2047
Eco57I	CTGAAG	2 cut(s)	114, 693
Eco81I	CCTNAGG	1 cut(s)	755
Eco88I	CYCGRG	3 cut(s)	852, 1223, 2048
EcoICRI	GAGCTC	1 cut(s)	2047
EcoO109I	RGGNCCY	1 cut(s)	1280
EcoRI	GAATTC	2 cut(s)	352, 973
EcoRII	CCWGG	6 cut(s)	774, 814, 841, 891, 1313, 1471
EcoT14I	CCWWGG	1 cut(s)	1169
EcoT38I	GRGCYC	1 cut(s)	2049
ErhI	CCWWGG	1 cut(s)	1169
FaeI	CATG	9 cut(s)	344, 496, 850, 875, 1168, 1269, 1610, 1805, 1988
FalI	AAGNNNNNCTT	2 cut(s)	8, 40
FaqI	GGGAC	3 cut(s)	1073, 1509, 1539
FatI	CATG	9 cut(s)	340, 492, 846, 871, 1164, 1265, 1606, 1801, 1984
FauNDI	CATATG	1 cut(s)	1045
Fnu4HI	GCNGC	6 cut(s)	123, 126, 254, 433, 498, 1712
FokI	GGATG	6 cut(s)	693, 908, 946, 1198, 1716, 1882
FriOI	GRGCYC	1 cut(s)	2049
Fsp4HI	GCNGC	6 cut(s)	123, 126, 254, 433, 498, 1712
FspBI	CTAG	5 cut(s)	201, 662, 1208, 1815, 1928
GluI	GCNGC	6 cut(s)	123, 126, 254, 433, 498, 1712
HaeIII	GGCC	4 cut(s)	104, 1101, 1313, 1318
HapII	CCGG	7 cut(s)	230, 575, 763, 853, 1224, 1310, 1739
HgaI	GACGC	1 cut(s)	538
Hin1I	GRCGYC	1 cut(s)	513
Hin1II	CATG	9 cut(s)	344, 496, 850, 875, 1168, 1269, 1610, 1805, 1988
HindIII	AAGCTT	4 cut(s)	181, 962, 1589, 1692
HinfI	GANTC	9 cut(s)	108, 245, 293, 406, 1228, 1533, 1550, 1845, 1940
HpaII	CCGG	7 cut(s)	230, 575, 763, 853, 1224, 1310, 1739
HphI	GGTGA	4 cut(s)	188, 594, 982, 1788
Hpy166II	GTNNAC	4 cut(s)	91, 901, 1232, 1852
Hpy188I	TCNGA	4 cut(s)	133, 298, 673, 723
Hpy8I	GTNNAC	4 cut(s)	91, 901, 1232, 1852
HpyAV	CCTTC	4 cut(s)	110, 210, 668, 1331
HpyCH4III	ACNGT	1 cut(s)	1387
HpyCH4IV	ACGT	5 cut(s)	398, 513, 943, 1109, 1790
HpyF10VI	GCNNNNNNNGC	4 cut(s)	97, 438, 749, 1174
HpyF3I	CTNAG	7 cut(s)	411, 450, 728, 755, 1160, 1178, 2025
HpySE526I	ACGT	5 cut(s)	398, 513, 943, 1109, 1790
Hsp92I	GRCGYC	1 cut(s)	513
Hsp92II	CATG	9 cut(s)	344, 496, 850, 875, 1168, 1269, 1610, 1805, 1988
Kzo9I	GATC	9 cut(s)	345, 414, 706, 1113, 1213, 1703, 1762, 1981, 2035
LguI	GCTCTTC	2 cut(s)	160, 2037
LmnI	GCTCC	2 cut(s)	817, 1174
Lsp1109I	GCAGC	5 cut(s)	109, 112, 265, 444, 509
LweI	GCATC	2 cut(s)	247, 671
MaeI	CTAG	5 cut(s)	201, 662, 1208, 1815, 1928
MaeII	ACGT	5 cut(s)	398, 513, 943, 1109, 1790
MalI	GATC	9 cut(s)	347, 416, 708, 1115, 1215, 1705, 1764, 1983, 2037
MboI	GATC	9 cut(s)	345, 414, 706, 1113, 1213, 1703, 1762, 1981, 2035
MfeI	CAATTG	1 cut(s)	1431
MflI	RGATCY	2 cut(s)	414, 1762
MhlI	GDGCHC	3 cut(s)	93, 1854, 2049
MlsI	TGGCCA	2 cut(s)	104, 1101
MluNI	TGGCCA	2 cut(s)	104, 1101
MlyI	GAGTC	4 cut(s)	1237, 1527, 1854, 1934
MmeI	TCCRAC	2 cut(s)	28, 2010
Mox20I	TGGCCA	2 cut(s)	104, 1101
MroXI	GAANNNNTTC	2 cut(s)	912, 964
MscI	TGGCCA	2 cut(s)	104, 1101
MseI	TTAA	8 cut(s)	390, 456, 546, 582, 806, 993, 1685, 1754
MslI	CAYNNNNRTG	3 cut(s)	575, 876, 1989
Msp20I	TGGCCA	2 cut(s)	104, 1101
MspA1I	CMGCKG	2 cut(s)	1547, 1714
MspCI	CTTAAG	1 cut(s)	1753
MspI	CCGG	7 cut(s)	230, 575, 763, 853, 1224, 1310, 1739
MunI	CAATTG	1 cut(s)	1431
Mva1269I	GAATGC	1 cut(s)	265
MvaI	CCWGG	6 cut(s)	776, 816, 843, 893, 1315, 1473
MwoI	GCNNNNNNNGC	4 cut(s)	97, 438, 749, 1174
NciI	CCSGG	6 cut(s)	853, 854, 1224, 1225, 1310, 1739
NdeI	CATATG	1 cut(s)	1045
NdeII	GATC	9 cut(s)	345, 414, 706, 1113, 1213, 1703, 1762, 1981, 2035
NlaIII	CATG	9 cut(s)	344, 496, 850, 875, 1168, 1269, 1610, 1805, 1988
NlaIV	GGNNCC	3 cut(s)	228, 761, 813
NspI	RCATGY	1 cut(s)	1168
PaeI	GCATGC	1 cut(s)	1168
PaeR7I	CTCGAG	1 cut(s)	2048
PagI	TCATGA	3 cut(s)	340, 1606, 1984
PciSI	GCTCTTC	2 cut(s)	160, 2037
PctI	GAATGC	1 cut(s)	265
PdmI	GAANNNNTTC	2 cut(s)	912, 964
PfeI	GAWTC	5 cut(s)	108, 245, 293, 406, 1550
PflMI	CCANNNNNTGG	1 cut(s)	493
PfoI	TCCNGGA	4 cut(s)	774, 814, 891, 1737
PkrI	GCNGC	6 cut(s)	124, 127, 255, 434, 499, 1713
Ple19I	CGATCG	1 cut(s)	1216
PleI	GAGTC	4 cut(s)	1236, 1527, 1853, 1934
PpsI	GAGTC	4 cut(s)	1236, 1527, 1853, 1934
PpuMI	RGGWCCY	1 cut(s)	1280
PshBI	ATTAAT	1 cut(s)	1685
Psp124BI	GAGCTC	1 cut(s)	2049
Psp1406I	AACGTT	1 cut(s)	398
Psp5II	RGGWCCY	1 cut(s)	1280
Psp6I	CCWGG	6 cut(s)	774, 814, 841, 891, 1313, 1471
PspGI	CCWGG	6 cut(s)	774, 814, 841, 891, 1313, 1471
PspN4I	GGNNCC	3 cut(s)	228, 761, 813
PspPI	GGNCC	6 cut(s)	227, 484, 772, 1280, 1317, 1401
PspPPI	RGGWCCY	1 cut(s)	1280
PspXI	VCTCGAGB	1 cut(s)	2048
PstNI	CAGNNNCTG	1 cut(s)	727
PsuI	RGATCY	2 cut(s)	414, 1762
PvuI	CGATCG	1 cut(s)	1216
PvuII	CAGCTG	1 cut(s)	1547
RsaI	GTAC	4 cut(s)	900, 1052, 1384, 1650
RsaNI	GTAC	4 cut(s)	899, 1051, 1383, 1649
RseI	CAYNNNNRTG	3 cut(s)	575, 876, 1989
SacI	GAGCTC	1 cut(s)	2049
SapI	GCTCTTC	2 cut(s)	160, 2037
SaqAI	TTAA	8 cut(s)	390, 456, 546, 582, 806, 993, 1685, 1754
SatI	GCNGC	6 cut(s)	123, 126, 254, 433, 498, 1712
Sau3AI	GATC	9 cut(s)	345, 414, 706, 1113, 1213, 1703, 1762, 1981, 2035
Sau96I	GGNCC	6 cut(s)	227, 484, 772, 1280, 1317, 1401
SchI	GAGTC	4 cut(s)	1237, 1527, 1854, 1934
SduI	GDGCHC	3 cut(s)	93, 1854, 2049
SfaNI	GCATC	2 cut(s)	247, 671
Sfr274I	CTCGAG	1 cut(s)	2048
SinI	GGWCC	5 cut(s)	227, 484, 772, 1280, 1401
SlaI	CTCGAG	1 cut(s)	2048
SmaI	CCCGGG	2 cut(s)	854, 1225
SmiMI	CAYNNNNRTG	3 cut(s)	575, 876, 1989
SmlI	CTYRAG	4 cut(s)	25, 1753, 1824, 2048
SmoI	CTYRAG	4 cut(s)	25, 1753, 1824, 2048
SpeI	ACTAGT	2 cut(s)	200, 661
SphI	GCATGC	1 cut(s)	1168
SsiI	CCGC	4 cut(s)	333, 1358, 1698, 1712
SspMI	CTAG	5 cut(s)	201, 662, 1208, 1815, 1928
SstI	GAGCTC	1 cut(s)	2049
StyI	CCWWGG	1 cut(s)	1169
TaaI	ACNGT	1 cut(s)	1387
TaiI	ACGT	5 cut(s)	401, 516, 946, 1112, 1793
TaqI	TCGA	5 cut(s)	243, 356, 1212, 1895, 2049
TatI	WGTACW	2 cut(s)	898, 1382
TauI	GCSGC	1 cut(s)	1714
TfiI	GAWTC	5 cut(s)	108, 245, 293, 406, 1550
Tru1I	TTAA	8 cut(s)	390, 456, 546, 582, 806, 993, 1685, 1754
Tru9I	TTAA	8 cut(s)	390, 456, 546, 582, 806, 993, 1685, 1754
TseI	GCWGC	5 cut(s)	122, 125, 253, 432, 497
TspGWI	ACGGA	2 cut(s)	920, 1955
TspMI	CCCGGG	2 cut(s)	852, 1223
Van91I	CCANNNNNTGG	1 cut(s)	493
Vha464I	CTTAAG	1 cut(s)	1753
VneI	GTGCAC	2 cut(s)	89, 1850
VpaK11BI	GGWCC	5 cut(s)	227, 484, 772, 1280, 1401
VspI	ATTAAT	1 cut(s)	1685
XapI	RAATTY	6 cut(s)	187, 352, 792, 973, 1504, 1602
XbaI	TCTAGA	1 cut(s)	1927
XceI	RCATGY	1 cut(s)	1168
XhoI	CTCGAG	1 cut(s)	2048
XmaI	CCCGGG	2 cut(s)	852, 1223
XmnI	GAANNNNTTC	2 cut(s)	912, 964
XspI	CTAG	5 cut(s)	201, 662, 1208, 1815, 1928
ZraI	GACGTC	1 cut(s)	514

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.