Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0003954

Physical Location & Seq

Genomic Coordinates Reverse (-)

6313839 .. 6319368

Gene Structure 5530 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1087900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2184 bp

ATGGATGGGAACAAGCGAAACTTTTTCAAGAAACGCCCTAACATCCAGTTTAAAAGAAAAGGGGGTAACAACAACTATAAGAAAGGAAAGTGGAATGAATCAGGCCGTGAGCAACCTCTAGAGGATTACCAATCGCTCGACACTGTGTATCGTATTCTCTGCCCTTCTAAAAAGATCGGTGGTGTTATTGGGAAGGGTGGCAACATAGTCAAAGCCCTTAGAGAAGAGACCCGGGCAAAGATTTCGGTTTCTGATTCTGTTCTTGGCTCAGATGAGAGAGTAATTATTATCTATAGCTCTCCAACAAAAATTTCAAGCAAACAAAATAACAATGAAGATTCAGATGAGGGAAATGAAATGGAGCCACATTGTGCTGCCCAGAATGCTCTTTTGAAAGTTCATGCCAGGATTGTAGAGGAAGATCTCTTTGGTGGAGTTACATTTGATGATGACAACGAGAACAGTGTTGTCACGACAAGACTTCTAGTTCCAAACAATACGGTAGGGTGTGTTCTTGGAAAGCGTGGAGATGTTATTCAAAGATTGCGAAGTGAGACGGGTGCAAGTATCCGTGTCCTTCCTGCAGATCAGCTTCCAACTTGTGCTAAGGAAACTGATGAATTGGTGCAGATATCTGGAAAACTAAATGTGACAAAGAGGGCACTTTACGAAGTGTCAACTCTACTGCATCAGAATCCTAGGAAGGATAAGCCTCCTTCAGGCTTTCCTATGCCTATTGGTGGTCAGGGGTTTCATCCCCCTGGAGACCCGATGACTAATGCCCTTTCACCCAGAAATCCAGTGTTGTCTAATCGGGCTCCTTCCCATACTATGCCGCCAATGCCATGGACAGGAGGATATGAAAACCGTTCTTCTGGATTTGCCCGAGGTGGTTTTAATGGGGTTTCTCCTGAACATGGGGGTGAAGCTCTAGCTCTAGCTGAGTTTTCTATGAAAATTTTGTGTTCAACTGGGAAAATTGGTGGGGTTATTGGCAAGGGCGGTTTTAATGTAAAACAGTTACAACTGGAAACAGGAGCCACTATTCATGTTCAGGATGCATCAACGGATTCAGAAGAACGTGTAATTCACGTCTCTGCTTTTGAGGCTTTGTGGAATCCCAGATCACAGACCATTGAGGCAATTCTTGAGCTTCAGAACATAACAAGTGAATTCTCTGATAAAGGCATAATTGCTACCAGGCTTCTTGTACCATCAAGTAAAGTTGGCTGCCTCCTTGGACAAGGAGGTCAAGTTATAAATGAGATGAGAAGGAGAACCCAGGCTGATATCCGTGTTTACTCAAAGGATGATAGGCCTGCATGTGCAGCTGAAGATGAAGAGCTTGTGCAGATTTCTGGGAACTTGGGTACCACTAAAGATGCTTTGGCTGAGATTACGTCAAGGCTGAGAGTGAGAACCCTGCGAGATACAAATGCTGGAGCAGAACCTGCTCCTGTTGGCCCTACGCACAGATTTGGTCCTGCAGGCCACTTACCAGGTGGGGGACCACTGCCAACACCTAGTGCAGTTGGAGCTGGAAGCTCTGGTGGATATGATCCTCTGAAGGTTAGTAGACATGAATATCAACCAGAGAGTCATCGAATTCCTCCAACTGCTTCTGGTTATCCAAGGGTCAACTATGCTATGGAGGGTAAGATCCCAACGAATGCAGTCGATTTTCCTGGAACAGTAGGGCGCAGCTTTTCCACTATTAGGGAGGTTGGCGTATCAAGAGCGCAGCTCCAAGACTCCCAAGGTGGTGGCTTTGAACGTTCTGTTGAGATTGGCGGTTCTGATCACTGGAATGCAGCACAGAATATCCTTCGGCCATTCTTGGCTTCTGCGGATCAAAATGTGAGTTCTCAATCTGGTCCCTACCGCAATGTAAATGCTCCACAAGGCTCCTACCACAAGATGATTCCTCAGCAAAGCCCCTACCAAATCAATTCTCAACAAAGCCCCTACCAACTCAATCCTCAGCACAGCTCATACCGAATCAATCCTCAGCATAGCCCCTATCAAGTCAATGCTTCGCACAGCCCCTTCCGAACAAATGGTCAGGAAAGCCCCTATCAATTGAGCTCTCAACAAGGATCATACCCCAACACGAATGCTCCTCAAGGCACCTACCACAACTATAATTCGCAACAAGGTGCCTACCAATACCATTACCGGCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KH_1

Protein Analysis

728

Amino Acids

79.13

Weight (kDa)

8.79

Isoelectric Point (pI)

52.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KH_1	PF00013	50 - 104	1.3e-12	KH domain
KH_1	PF00013	158 - 225	9.6e-14	KH domain
KH_1	PF00013	318 - 367	6.1e-10	KH domain
KH_1	PF00013	400 - 467	5.4e-13	KH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003954)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g45170 FvH4_6g45170 FvH4_6g45170 FvH4_6g45170 FvH4_6g45170 FvH4_6g45170 FvH4_6g45170
malus_domestica	MD09G1087900.v1.1 MD17G1076800.v1.1 MD17G1076900.v1.1 MD17G1077600.v1.1
prunus_persica	Prupe.3G237700_v2.0.a1 Prupe.3G237700_v2.0.a1 Prupe.3G237700_v2.0.a1 Prupe.3G237700_v2.0.a1 Prupe.3G237700_v2.0.a1 Prupe.3G237700_v2.0.a1
pyrus_communis	pycom09g01340 pycom17g07720
rosa_chinensis	RchiOBHm_Chr2g0162761 RchiOBHm_Chr2g0162821 RchiOBHm_Chr2g0162871
rosa_laevigata	RLG00000021358
rosa_multiflora	Rmu_co8424937.1_g000001 Rmu_sc0006129.1_g000028
rosa_roxburghii	Rroxscaffold_2G00087750
rosa_rugosa	Rorug02G0498900 Rorug02G0498900 Rorug02G0499000
rosa_samantha	Rh2AG564900 Rh2BG577600 Rh2CG547400 Rh2DG587500
rosa_wichuraiana	Rw2G046750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1259
AasI	GACNNNNNNGTC	1 cut(s)	1248
Acc36I	ACCTGC	1 cut(s)	1459
Acc65I	GGTACC	1 cut(s)	1370
AccB1I	GGYRCC	3 cut(s)	1370, 2126, 2156
AccI	GTMKAC	1 cut(s)	1576
AciI	CCGC	5 cut(s)	836, 1002, 1791, 1847, 1882
AclI	AACGTT	1 cut(s)	1774
AclWI	GGATC	4 cut(s)	1553, 1654, 1857, 2104
AcoI	YGGCCR	1 cut(s)	1829
AcsI	RAATTY	4 cut(s)	309, 957, 1172, 1605
AcuI	CTGAAG	4 cut(s)	702, 1139, 1353, 1586
AdeI	CACNNNGTG	2 cut(s)	371, 1526
AfaI	GTAC	2 cut(s)	1212, 1372
AfiI	CCNNNNNNNGG	6 cut(s)	719, 740, 851, 917, 1505, 1716
AflIII	ACRYGT	1 cut(s)	1081
AgsI	TTSAA	6 cut(s)	28, 315, 394, 539, 969, 1772
AjiI	CACGTC	1 cut(s)	1093
AjnI	CCWGG	6 cut(s)	404, 760, 1199, 1281, 1498, 1684
AjuI	GAANNNNNNNTTGG	4 cut(s)	295, 327, 411, 443
Alw21I	GWGCWC	1 cut(s)	2087
Alw26I	GTCTC	4 cut(s)	221, 548, 759, 1099
AlwI	GGATC	4 cut(s)	1553, 1654, 1857, 2104
AlwNI	CAGNNNCTG	1 cut(s)	1451
Ama87I	CYCGRG	2 cut(s)	231, 885
AoxI	GGCC	5 cut(s)	103, 1316, 1462, 1489, 1829
ApeKI	GCWGC	6 cut(s)	374, 1230, 1328, 1701, 1741, 1811
ApoI	RAATTY	4 cut(s)	309, 957, 1172, 1605
ArsI	GACNNNNNNTTYG	2 cut(s)	2044, 2076
Asp718I	GGTACC	1 cut(s)	1370
AspA2I	CCTAGG	1 cut(s)	698
AspLEI	GCGC	2 cut(s)	1701, 1741
AspS9I	GGNCC	4 cut(s)	1463, 1481, 1508, 1874
AsuC2I	CCSGG	2 cut(s)	232, 233
AsuHPI	GGTGA	2 cut(s)	780, 935
AvaI	CYCGRG	2 cut(s)	231, 885
AvaII	GGWCC	3 cut(s)	1481, 1508, 1874
AvrII	CCTAGG	1 cut(s)	698
BaeGI	GKGCMC	1 cut(s)	664
BaeI	ACNNNNGTAYC	2 cut(s)	1354, 1387
BanI	GGYRCC	3 cut(s)	1370, 2126, 2156
BanII	GRGCYC	2 cut(s)	820, 2087
Bbv12I	GWGCWC	1 cut(s)	2087
BbvCI	CCTCAGC	3 cut(s)	1926, 1980, 2007
BbvI	GCAGC	6 cut(s)	361, 1217, 1340, 1713, 1753, 1823
BccI	CCATC	1 cut(s)	1222
BceAI	ACGGC	1 cut(s)	90
BcgI	CGANNNNNNTGC	2 cut(s)	225, 259
BciT130I	CCWGG	6 cut(s)	406, 762, 1201, 1283, 1500, 1686
BciVI	GTATCC	1 cut(s)	578
BclI	TGATCA	1 cut(s)	1798
BcnI	CCSGG	2 cut(s)	232, 233
BcoDI	GTCTC	4 cut(s)	221, 548, 759, 1099
BfaI	CTAG	7 cut(s)	119, 485, 699, 932, 938, 1524, 2182
BfmI	CTRYAG	3 cut(s)	292, 582, 1485
BfuAI	ACCTGC	1 cut(s)	1459
BfuI	GTATCC	1 cut(s)	578
BglII	AGATCT	1 cut(s)	421
BisI	GCNGC	7 cut(s)	375, 836, 1231, 1329, 1702, 1742, 1812
BlnI	CCTAGG	1 cut(s)	698
BlsI	GCNGC	7 cut(s)	376, 837, 1232, 1330, 1703, 1743, 1813
Bme1390I	CCNGG	8 cut(s)	232, 233, 406, 762, 1201, 1283, 1500, 1686
Bme18I	GGWCC	3 cut(s)	1481, 1508, 1874
BmeT110I	CYCGRG	2 cut(s)	231, 885
BmgBI	CACGTC	1 cut(s)	1093
BmgT120I	GGNCC	4 cut(s)	1463, 1481, 1508, 1874
BmiI	GGNNCC	9 cut(s)	363, 819, 1039, 1372, 1509, 1876, 1906, 2128, 2158
BmrFI	CCNGG	8 cut(s)	232, 233, 406, 762, 1201, 1283, 1500, 1686
BmrI	ACTGGG	1 cut(s)	981
BmsI	GCATC	4 cut(s)	697, 1048, 1070, 1372
BmuI	ACTGGG	1 cut(s)	981
BplI	GAGNNNNNCTC	2 cut(s)	1728, 1760
BpmI	CTGGAG	2 cut(s)	783, 1461
Bpu10I	CCTNAGC	4 cut(s)	606, 1926, 1980, 2007
BpuEI	CTTGAG	2 cut(s)	1169, 2106
BpuMI	CCSGG	2 cut(s)	232, 233
BsaI	GGTCTC	2 cut(s)	221, 759
BsaJI	CCNNGG	9 cut(s)	231, 698, 760, 845, 886, 1237, 1281, 1631, 1756
BsaXI	ACNNNNNCTCC	4 cut(s)	1239, 1269, 2101, 2131
Bsc4I	CCNNNNNNNGG	6 cut(s)	719, 740, 851, 917, 1505, 1716
Bse118I	RCCGGY	1 cut(s)	2175
Bse1I	ACTGG	5 cut(s)	46, 800, 976, 1032, 1808
Bse3DI	GCAATG	1 cut(s)	1891
BseBI	CCWGG	6 cut(s)	406, 762, 1201, 1283, 1500, 1686
BseDI	CCNNGG	9 cut(s)	231, 698, 760, 845, 886, 1237, 1281, 1631, 1756
BseGI	GGATG	5 cut(s)	10, 42, 754, 1063, 1315
BseLI	CCNNNNNNNGG	6 cut(s)	719, 740, 851, 917, 1505, 1716
BseMI	GCAATG	1 cut(s)	1891
BseMII	CTCAG	7 cut(s)	282, 933, 1383, 1400, 1940, 1994, 2021
BseNI	ACTGG	5 cut(s)	46, 800, 976, 1032, 1808
BseRI	GAGGAG	1 cut(s)	2109
BseSI	GKGCMC	1 cut(s)	664
BseXI	GCAGC	6 cut(s)	361, 1217, 1340, 1713, 1753, 1823
BsgI	GTGCAG	4 cut(s)	647, 1347, 1370, 1548
BshFI	GGCC	5 cut(s)	105, 1318, 1464, 1491, 1831
BshNI	GGYRCC	3 cut(s)	1370, 2126, 2156
BsiHKAI	GWGCWC	1 cut(s)	2087
BsiHKCI	CYCGRG	2 cut(s)	231, 885
BsiSI	CCGG	2 cut(s)	232, 2176
BslFI	GGGAC	2 cut(s)	1521, 1860
BslI	CCNNNNNNNGG	6 cut(s)	719, 740, 851, 917, 1505, 1716
BsmAI	GTCTC	4 cut(s)	221, 548, 759, 1099
BsmBI	CGTCTC	2 cut(s)	548, 1099
BsmFI	GGGAC	2 cut(s)	1521, 1860
BsmI	GAATGC	4 cut(s)	388, 1675, 1813, 2119
BsnI	GGCC	5 cut(s)	105, 1318, 1464, 1491, 1831
Bso31I	GGTCTC	2 cut(s)	221, 759
BsoBI	CYCGRG	2 cut(s)	231, 885
Bsp1286I	GDGCHC	3 cut(s)	664, 820, 2087
Bsp143I	GATC	9 cut(s)	174, 421, 586, 1124, 1558, 1659, 1798, 1849, 2096
Bsp19I	CCATGG	1 cut(s)	845
BspACI	CCGC	5 cut(s)	836, 1002, 1791, 1847, 1882
BspANI	GGCC	5 cut(s)	105, 1318, 1464, 1491, 1831
BspCNI	CTCAG	7 cut(s)	281, 934, 1384, 1401, 1939, 1993, 2020
BspLI	GGNNCC	9 cut(s)	363, 819, 1039, 1372, 1509, 1876, 1906, 2128, 2158
BspMAI	CTGCAG	2 cut(s)	586, 1489
BspMI	ACCTGC	1 cut(s)	1459
BspPI	GGATC	4 cut(s)	1553, 1654, 1857, 2104
BspQI	GCTCTTC	1 cut(s)	1335
BspT107I	GGYRCC	3 cut(s)	1370, 2126, 2156
BspTNI	GGTCTC	2 cut(s)	221, 759
BsrDI	GCAATG	1 cut(s)	1891
BsrFI	RCCGGY	1 cut(s)	2175
BsrI	ACTGG	5 cut(s)	46, 800, 976, 1032, 1808
BssAI	RCCGGY	1 cut(s)	2175
BssECI	CCNNGG	9 cut(s)	231, 698, 760, 845, 886, 1237, 1281, 1631, 1756
BssMI	GATC	9 cut(s)	174, 421, 586, 1124, 1558, 1659, 1798, 1849, 2096
BssT1I	CCWWGG	5 cut(s)	698, 845, 1237, 1631, 1756
Bst2UI	CCWGG	6 cut(s)	406, 762, 1201, 1283, 1500, 1686
Bst4CI	ACNGT	6 cut(s)	145, 464, 502, 869, 1020, 1693
Bst6I	CTCTTC	2 cut(s)	219, 1335
BstAPI	GCANNNNNTGC	1 cut(s)	1451
BstC8I	GCNNGC	2 cut(s)	1320, 1489
BstDEI	CTNAG	9 cut(s)	218, 268, 606, 942, 1392, 1409, 1926, 1980, 2007
BstDSI	CCRYGG	1 cut(s)	845
BstENI	CCTNNNNNAGG	1 cut(s)	717
BstF5I	GGATG	5 cut(s)	10, 42, 754, 1063, 1315
BstHHI	GCGC	2 cut(s)	1701, 1741
BstKTI	GATC	9 cut(s)	177, 424, 589, 1127, 1561, 1662, 1801, 1852, 2099
BstMAI	GTCTC	4 cut(s)	221, 548, 759, 1099
BstMBI	GATC	9 cut(s)	174, 421, 586, 1124, 1558, 1659, 1798, 1849, 2096
BstMWI	GCNNNNNNNGC	6 cut(s)	383, 841, 1106, 1328, 1451, 1535
BstNI	CCWGG	6 cut(s)	406, 762, 1201, 1283, 1500, 1686
BstNSI	RCATGY	1 cut(s)	1326
BstSCI	CCNGG	8 cut(s)	230, 231, 404, 760, 1199, 1281, 1498, 1684
BstSFI	CTRYAG	3 cut(s)	292, 582, 1485
BstSLI	GKGCMC	1 cut(s)	664
BstV1I	GCAGC	6 cut(s)	361, 1217, 1340, 1713, 1753, 1823
BstX2I	RGATCY	2 cut(s)	421, 1659
BstXI	CCANNNNNNTGG	2 cut(s)	846, 1763
BstYI	RGATCY	2 cut(s)	421, 1659
BsuI	GTATCC	1 cut(s)	578
BsuRI	GGCC	5 cut(s)	105, 1318, 1464, 1491, 1831
BtgI	CCRYGG	1 cut(s)	845
BtrI	CACGTC	1 cut(s)	1093
BtsCI	GGATG	5 cut(s)	10, 42, 754, 1063, 1315
BtsI	GCAGTG	1 cut(s)	1511
BtsIMutI	CAGTG	5 cut(s)	141, 469, 807, 1511, 1801
BveI	ACCTGC	1 cut(s)	1459
Cac8I	GCNNGC	2 cut(s)	1320, 1489
CaiI	CAGNNNCTG	1 cut(s)	1451
CfoI	GCGC	2 cut(s)	1701, 1741
Cfr10I	RCCGGY	1 cut(s)	2175
Cfr13I	GGNCC	4 cut(s)	1463, 1481, 1508, 1874
Cfr9I	CCCGGG	1 cut(s)	231
CsiI	ACCWGGT	1 cut(s)	1498
Csp6I	GTAC	2 cut(s)	1211, 1371
CviAII	CATG	6 cut(s)	401, 846, 917, 1049, 1323, 1580
CviQI	GTAC	2 cut(s)	1211, 1371
DdeI	CTNAG	9 cut(s)	218, 268, 606, 942, 1392, 1409, 1926, 1980, 2007
DpnI	GATC	9 cut(s)	176, 423, 588, 1126, 1560, 1661, 1800, 1851, 2098
DpnII	GATC	9 cut(s)	174, 421, 586, 1124, 1558, 1659, 1798, 1849, 2096
DraI	TTTAAA	1 cut(s)	52
DraIII	CACNNNGTG	2 cut(s)	371, 1526
DrdI	GACNNNNNNGTC	1 cut(s)	1248
DseDI	GACNNNNNNGTC	1 cut(s)	1248
EaeI	YGGCCR	1 cut(s)	1829
Eam1104I	CTCTTC	2 cut(s)	219, 1335
EarI	CTCTTC	2 cut(s)	219, 1335
Ecl136II	GAGCTC	1 cut(s)	2085
Eco130I	CCWWGG	5 cut(s)	698, 845, 1237, 1631, 1756
Eco147I	AGGCCT	1 cut(s)	1318
Eco24I	GRGCYC	2 cut(s)	820, 2087
Eco31I	GGTCTC	2 cut(s)	221, 759
Eco32I	GATATC	2 cut(s)	633, 1291
Eco47I	GGWCC	3 cut(s)	1481, 1508, 1874
Eco53kI	GAGCTC	1 cut(s)	2085
Eco57I	CTGAAG	4 cut(s)	702, 1139, 1353, 1586
Eco88I	CYCGRG	2 cut(s)	231, 885
EcoICRI	GAGCTC	1 cut(s)	2085
EcoNI	CCTNNNNNAGG	1 cut(s)	717
EcoRI	GAATTC	2 cut(s)	1172, 1605
EcoRII	CCWGG	6 cut(s)	404, 760, 1199, 1281, 1498, 1684
EcoRV	GATATC	2 cut(s)	633, 1291
EcoT14I	CCWWGG	5 cut(s)	698, 845, 1237, 1631, 1756
EcoT22I	ATGCAT	1 cut(s)	1063
EcoT38I	GRGCYC	2 cut(s)	820, 2087
ErhI	CCWWGG	5 cut(s)	698, 845, 1237, 1631, 1756
Esp3I	CGTCTC	2 cut(s)	548, 1099
FaeI	CATG	6 cut(s)	404, 849, 920, 1052, 1326, 1583
FalI	AAGNNNNNCTT	1 cut(s)	37
FaqI	GGGAC	2 cut(s)	1521, 1860
FatI	CATG	6 cut(s)	400, 845, 916, 1048, 1322, 1579
FbaI	TGATCA	1 cut(s)	1798
FblI	GTMKAC	1 cut(s)	1576
Fnu4HI	GCNGC	7 cut(s)	375, 836, 1231, 1329, 1702, 1742, 1812
FokI	GGATG	5 cut(s)	17, 29, 741, 1070, 1322
FriOI	GRGCYC	2 cut(s)	820, 2087
Fsp4HI	GCNGC	7 cut(s)	375, 836, 1231, 1329, 1702, 1742, 1812
FspBI	CTAG	7 cut(s)	119, 485, 699, 932, 938, 1524, 2182
GlaI	GCGC	2 cut(s)	1700, 1740
GluI	GCNGC	7 cut(s)	375, 836, 1231, 1329, 1702, 1742, 1812
GsuI	CTGGAG	2 cut(s)	783, 1461
HaeIII	GGCC	5 cut(s)	105, 1318, 1464, 1491, 1831
HapII	CCGG	2 cut(s)	232, 2176
HhaI	GCGC	2 cut(s)	1701, 1741
Hin1II	CATG	6 cut(s)	404, 849, 920, 1052, 1326, 1583
Hin6I	GCGC	2 cut(s)	1699, 1739
HinP1I	GCGC	2 cut(s)	1699, 1739
HincII	GTYRAC	2 cut(s)	678, 1639
HindII	GTYRAC	2 cut(s)	678, 1639
HpaII	CCGG	2 cut(s)	232, 2176
HphI	GGTGA	2 cut(s)	780, 935
Hpy166II	GTNNAC	4 cut(s)	678, 1300, 1577, 1639
Hpy8I	GTNNAC	4 cut(s)	678, 1300, 1577, 1639
HpyCH4III	ACNGT	6 cut(s)	145, 464, 502, 869, 1020, 1693
HpyCH4IV	ACGT	4 cut(s)	1081, 1092, 1400, 1774
HpyF10VI	GCNNNNNNNGC	6 cut(s)	383, 841, 1106, 1328, 1451, 1535
HpyF3I	CTNAG	9 cut(s)	218, 268, 606, 942, 1392, 1409, 1926, 1980, 2007
HpySE526I	ACGT	4 cut(s)	1081, 1092, 1400, 1774
Hsp92II	CATG	6 cut(s)	404, 849, 920, 1052, 1326, 1583
HspAI	GCGC	2 cut(s)	1699, 1739
KpnI	GGTACC	1 cut(s)	1374
Ksp22I	TGATCA	1 cut(s)	1798
Kzo9I	GATC	9 cut(s)	174, 421, 586, 1124, 1558, 1659, 1798, 1849, 2096
LguI	GCTCTTC	1 cut(s)	1335
Lsp1109I	GCAGC	6 cut(s)	361, 1217, 1340, 1713, 1753, 1823
LweI	GCATC	4 cut(s)	697, 1048, 1070, 1372
MabI	ACCWGGT	1 cut(s)	1498
MaeI	CTAG	7 cut(s)	119, 485, 699, 932, 938, 1524, 2182
MaeII	ACGT	4 cut(s)	1081, 1092, 1400, 1774
MaeIII	GTNAC	5 cut(s)	65, 436, 469, 649, 1020
MalI	GATC	9 cut(s)	176, 423, 588, 1126, 1560, 1661, 1800, 1851, 2098
MboI	GATC	9 cut(s)	174, 421, 586, 1124, 1558, 1659, 1798, 1849, 2096
MboII	GAAGA	7 cut(s)	236, 347, 431, 864, 1088, 1346, 1352
MfeI	CAATTG	1 cut(s)	2078
MflI	RGATCY	2 cut(s)	421, 1659
MhlI	GDGCHC	3 cut(s)	664, 820, 2087
MlyI	GAGTC	2 cut(s)	1606, 1745
MmeI	TCCRAC	4 cut(s)	326, 620, 1513, 1637
Mph1103I	ATGCAT	1 cut(s)	1063
MseI	TTAA	3 cut(s)	51, 897, 1008
MslI	CAYNNNNRTG	2 cut(s)	921, 1806
MspA1I	CMGCKG	1 cut(s)	1331
MspI	CCGG	2 cut(s)	232, 2176
MspR9I	CCNGG	8 cut(s)	232, 233, 406, 762, 1201, 1283, 1500, 1686
MunI	CAATTG	1 cut(s)	2078
Mva1269I	GAATGC	4 cut(s)	388, 1675, 1813, 2119
MvaI	CCWGG	6 cut(s)	406, 762, 1201, 1283, 1500, 1686
MwoI	GCNNNNNNNGC	6 cut(s)	383, 841, 1106, 1328, 1451, 1535
NciI	CCSGG	2 cut(s)	232, 233
NcoI	CCATGG	1 cut(s)	845
NdeII	GATC	9 cut(s)	174, 421, 586, 1124, 1558, 1659, 1798, 1849, 2096
NlaIII	CATG	6 cut(s)	404, 849, 920, 1052, 1326, 1583
NlaIV	GGNNCC	9 cut(s)	363, 819, 1039, 1372, 1509, 1876, 1906, 2128, 2158
NmuCI	GTSAC	2 cut(s)	469, 649
NsiI	ATGCAT	1 cut(s)	1063
NspI	RCATGY	1 cut(s)	1326
PceI	AGGCCT	1 cut(s)	1318
PciSI	GCTCTTC	1 cut(s)	1335
PctI	GAATGC	4 cut(s)	388, 1675, 1813, 2119
PfeI	GAWTC	8 cut(s)	98, 254, 338, 694, 1070, 1117, 1921, 1998
PfoI	TCCNGGA	1 cut(s)	1684
PkrI	GCNGC	7 cut(s)	376, 837, 1232, 1330, 1703, 1743, 1813
PleI	GAGTC	2 cut(s)	1605, 1745
PpsI	GAGTC	2 cut(s)	1605, 1745
PsiI	TTATAA	1 cut(s)	1259
Psp124BI	GAGCTC	1 cut(s)	2087
Psp1406I	AACGTT	1 cut(s)	1774
Psp6I	CCWGG	6 cut(s)	404, 760, 1199, 1281, 1498, 1684
PspGI	CCWGG	6 cut(s)	404, 760, 1199, 1281, 1498, 1684
PspN4I	GGNNCC	9 cut(s)	363, 819, 1039, 1372, 1509, 1876, 1906, 2128, 2158
PspPI	GGNCC	4 cut(s)	1463, 1481, 1508, 1874
PsrI	GAACNNNNNNTAC	2 cut(s)	495, 527
PstI	CTGCAG	2 cut(s)	586, 1489
PstNI	CAGNNNCTG	1 cut(s)	1451
PsuI	RGATCY	2 cut(s)	421, 1659
PvuII	CAGCTG	1 cut(s)	1331
RsaI	GTAC	2 cut(s)	1212, 1372
RsaNI	GTAC	2 cut(s)	1211, 1371
RseI	CAYNNNNRTG	2 cut(s)	921, 1806
SacI	GAGCTC	1 cut(s)	2087
SapI	GCTCTTC	1 cut(s)	1335
SaqAI	TTAA	3 cut(s)	51, 897, 1008
SatI	GCNGC	7 cut(s)	375, 836, 1231, 1329, 1702, 1742, 1812
Sau3AI	GATC	9 cut(s)	174, 421, 586, 1124, 1558, 1659, 1798, 1849, 2096
Sau96I	GGNCC	4 cut(s)	1463, 1481, 1508, 1874
SbfI	CCTGCAGG	1 cut(s)	1489
SchI	GAGTC	2 cut(s)	1606, 1745
ScrFI	CCNGG	8 cut(s)	232, 233, 406, 762, 1201, 1283, 1500, 1686
SdaI	CCTGCAGG	1 cut(s)	1489
SduI	GDGCHC	3 cut(s)	664, 820, 2087
SexAI	ACCWGGT	1 cut(s)	1498
SfaNI	GCATC	4 cut(s)	697, 1048, 1070, 1372
SfcI	CTRYAG	3 cut(s)	292, 582, 1485
SinI	GGWCC	3 cut(s)	1481, 1508, 1874
SmaI	CCCGGG	1 cut(s)	233
SmiMI	CAYNNNNRTG	2 cut(s)	921, 1806
SmlI	CTYRAG	2 cut(s)	1148, 2121
SmoI	CTYRAG	2 cut(s)	1148, 2121
Sse8387I	CCTGCAGG	1 cut(s)	1489
SseBI	AGGCCT	1 cut(s)	1318
SsiI	CCGC	5 cut(s)	836, 1002, 1791, 1847, 1882
SspMI	CTAG	7 cut(s)	119, 485, 699, 932, 938, 1524, 2182
SstI	GAGCTC	1 cut(s)	2087
StuI	AGGCCT	1 cut(s)	1318
StyD4I	CCNGG	8 cut(s)	230, 231, 404, 760, 1199, 1281, 1498, 1684
StyI	CCWWGG	5 cut(s)	698, 845, 1237, 1631, 1756
TaaI	ACNGT	6 cut(s)	145, 464, 502, 869, 1020, 1693
TaiI	ACGT	4 cut(s)	1084, 1095, 1403, 1777
TaqI	TCGA	3 cut(s)	138, 1603, 1677
TauI	GCSGC	1 cut(s)	838
TfiI	GAWTC	8 cut(s)	98, 254, 338, 694, 1070, 1117, 1921, 1998
Tru1I	TTAA	3 cut(s)	51, 897, 1008
Tru9I	TTAA	3 cut(s)	51, 897, 1008
TscAI	CASTG	5 cut(s)	148, 469, 807, 1518, 1808
TseFI	GTSAC	2 cut(s)	469, 649
TseI	GCWGC	6 cut(s)	374, 1230, 1328, 1701, 1741, 1811
Tsp45I	GTSAC	2 cut(s)	469, 649
TspGWI	ACGGA	3 cut(s)	560, 1082, 1283
TspMI	CCCGGG	1 cut(s)	231
TspRI	CASTG	5 cut(s)	148, 469, 807, 1518, 1808
VpaK11BI	GGWCC	3 cut(s)	1481, 1508, 1874
XagI	CCTNNNNNAGG	1 cut(s)	717
XapI	RAATTY	4 cut(s)	309, 957, 1172, 1605
XbaI	TCTAGA	1 cut(s)	118
XceI	RCATGY	1 cut(s)	1326
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1499
XmaI	CCCGGG	1 cut(s)	231
XmaJI	CCTAGG	1 cut(s)	698
XmiI	GTMKAC	1 cut(s)	1576
XspI	CTAG	7 cut(s)	119, 485, 699, 932, 938, 1524, 2182
Zsp2I	ATGCAT	1 cut(s)	1063

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.