Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0008977

Physical Location & Seq

Genomic Coordinates Forward (+)

6942412 .. 6946660

Gene Structure 4249 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1096300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1743 bp

ATGACGATCTTGTCCTTCTTCACCAGAGCTTCAAGAGCTTTCAATGGCCACCCTGCTGCCTCCAAGCTTCTGGTGCTCTGTACTATCAGTAGCGGAGGTCTCCTGGCGTATGCAGAATCGCAGTCCAACGTTGGCTCTCCTATTGTTGATCCTAATCAAAACGAGCCTAGGAAAAAGAGAGTGGTGGTGCTTGGGACAGGATGGGCTGGTACTAGTTTCCTTAAGTACCTGGATGCTTCAGCTTATGATGTTCAGGTTGTTTCACCCCGAAATTATTTTGCGTTTACTCCTTTGTTACCGAGTGTCACATGTGGGACGGTTGAAGCACGAAGCATAGTAGAACCCATTCGAAATATCATAAAAAAGAGAAATGGAGAGATCAAATTTTGGGAAGCAGAATGCGTCAAGATTGATGCAGCAAATAAAAATGTTTCCTTGCGGGCTAATTTTGACAAAAACTTGGTGGGAAATAAGGAATTTTCCCTTGAATATGACTACTTGGTCATAGCTTTAGGAGCACAAGTGAACACTTTTAACACCCCTGGTGTCAAGGAGAACTGTCATTTTCTGAAGGAAGTGGAAGATGCTCAAAAGATACGTATGAGTGTGATAGATTGCTTTGAAATGGCTGTACTTCCAGGTCTAAGTGAAGAAGAGCGGAGGAGAAATCTTCATTTTGTAATTGTTGGAGGAGGTCCTACTGGTGTGGAATTTGCTGCAGAGCTGCATGATTATTTCCAAGAAGATTTAGTCAAGTTATATCCTATGGTTAAGGATCTTGTGAAAATAACAGTTATCCAATCTGGAGATCACATCTTGAACATGTTTGACGACAGAATTAGTACTTTTGCCGAGAAGAAGTTTACTAGAGATGGTATCGATGTTCAAACAGGATGTCGGGTTGTCAGTGTTTCTGATAAGGAAATCACGATGAAGGTGAAATCGAAGGGAGAGGTTTGCTCTATACCACATGGGTTGGTTGTGTGGTCTACTGGTGTTGGGACTCGTCCAGTTGTGAGGGACTTTATGCAACAAATTGGGCAGGCTGATAAACGTGTTCTAGTAACTGACGAATGGCTGCGTGTAAAGGGATGTGAAGATGTGTATGCCATTGGTGATTGTGCTTCAATTAGTCAACGTAAAATCATGGAAGATATCTTCACCATATTTAAAGCTGCGGACAAAGATAACTCTGGTACCTTAACAGTTGATGAATTTCGAGATGTAGTTGACGACATCATCATAAGGTACCCCCAAGTGGAACTCTATTTAAAGAGCAGGCATCTGCAGGATGTGACAGACCTATGGAAAGATCTCGATGGTAGTGATAAGAAGGAAGTGACCGTGGAAGAATTTAAATTAGCCCTTTCTCAAGTGGATTCACAGATGAAGAGTCTGCCTGCAACTGCTCAGGTTGCTGCGCAACAGGGAACATATCTTTCTAGTTGCTTTAACCGGAGAGAGCACTGCAAAGAGCATCCTGAAGGTCCGAGGCGTTTTAAAAGTTCTGGGCAACATGAGTTTCTTCCCTTTCGGTACAAGCATTTCGGGCAATTTGCTCCGTTGGGGGGAGAGCAAGCAGCAGCGGAGCTACCCGGAGACTGGGTTTCCATGGGTCATAGCACACAGTGGCTCTGGTATTCTGTATATGCAAGCAAGCAAGTGAGCTGGCGAACTAGGGTTCTTGTGGTATCGGACTGGACCAGGAGATTCATTTTCGGGAGAGATTCAAGTCGTATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005758 GO:0005777 GO:0008150 GO:0008152 GO:0016020 GO:0016491 GO:0019866 GO:0019898 GO:0031090 GO:0031312 GO:0031314 GO:0031966 GO:0031967 GO:0031970 GO:0031974 GO:0031975 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0055114 GO:0070013

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pyr_redox_2

Protein Analysis

581

Amino Acids

65.31

Weight (kDa)

6.87

Isoelectric Point (pI)

35.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pyr_redox_2	PF07992	60 - 382	2.6e-56	Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox	PF00070	225 - 308	1e-10	Pyridine nucleotide-disulphide oxidoreductase
EF-hand_7	PF13499	384 - 453	5.6e-07	EF-hand domain pair
NDH2_C	PF22366	515 - 576	1.6e-16	NDH2 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008977)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G28220 AT4G28220
fragaria_vesca	FvH4_6g44592 FvH4_6g44593 FvH4_6g44690
malus_domestica	MD09G1096300.v1.1
prunus_persica	Prupe.3G231400_v2.0.a1
pyrus_communis	pycom09g02080
rosa_chinensis	RchiOBHm_Chr2g0161881
rosa_laevigata	RLG00000021305
rosa_multiflora	Rmu_co8378237.1_g000001 Rmu_sc0008565.1_g000008 Rmu_sc0018381.1_g000008 Rmu_sc0028798.1_g000001
rosa_rugosa	Rorug02G0492800 Rorug02G0492900 Rorug02G0493000
rosa_samantha	Rh2AG558800 Rh2BG572200 Rh2CG542600 Rh2DG582100
rosa_wichuraiana	Rw2G046260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	10, 500
Acc16I	TGCGCA	1 cut(s)	1422
Acc65I	GGTACC	2 cut(s)	1196, 1248
AccB1I	GGYRCC	2 cut(s)	1196, 1248
AccBSI	CCGCTC	1 cut(s)	658
AccI	GTMKAC	1 cut(s)	989
AciI	CCGC	5 cut(s)	93, 439, 658, 1178, 1586
AclI	AACGTT	1 cut(s)	129
AclWI	GGATC	2 cut(s)	143, 783
AcoI	YGGCCR	1 cut(s)	46
AcsI	RAATTY	5 cut(s)	383, 476, 710, 1214, 1352
AcuI	CTGAAG	3 cut(s)	222, 590, 1503
AdeI	CACNNNGTG	1 cut(s)	1629
AfaI	GTAC	8 cut(s)	82, 211, 227, 633, 844, 1198, 1250, 1538
AfiI	CCNNNNNNNGG	3 cut(s)	1258, 1568, 1602
AflII	CTTAAG	1 cut(s)	221
AflIII	ACRYGT	3 cut(s)	308, 822, 1054
AgsI	TTSAA	9 cut(s)	33, 43, 323, 488, 623, 820, 887, 1128, 1731
AhlI	ACTAGT	1 cut(s)	212
AjnI	CCWGG	5 cut(s)	102, 228, 541, 637, 1703
AluBI	AGCT	9 cut(s)	29, 38, 67, 242, 509, 724, 1175, 1591, 1668
AluI	AGCT	9 cut(s)	29, 38, 67, 242, 509, 724, 1175, 1591, 1668
Alw21I	GWGCWC	3 cut(s)	78, 520, 1467
Alw26I	GTCTC	2 cut(s)	104, 1593
AlwI	GGATC	2 cut(s)	143, 783
AoxI	GGCC	1 cut(s)	46
ApeKI	GCWGC	9 cut(s)	56, 416, 716, 724, 1078, 1175, 1418, 1580, 1583
ApoI	RAATTY	5 cut(s)	383, 476, 710, 1214, 1352
ArsI	GACNNNNNNTTYG	2 cut(s)	880, 912
Asp700I	GAANNNNTTC	1 cut(s)	345
Asp718I	GGTACC	2 cut(s)	1196, 1248
AspA2I	CCTAGG	1 cut(s)	167
AspLEI	GCGC	1 cut(s)	1423
AspS9I	GGNCC	3 cut(s)	695, 1487, 1701
AsuC2I	CCSGG	1 cut(s)	1596
AsuHPI	GGTGA	5 cut(s)	13, 255, 949, 1127, 1153
AsuII	TTCGAA	1 cut(s)	349
AvaII	GGWCC	3 cut(s)	695, 1487, 1701
AvrII	CCTAGG	1 cut(s)	167
BaeI	ACNNNNGTAYC	2 cut(s)	1180, 1213
BalI	TGGCCA	1 cut(s)	48
BanI	GGYRCC	2 cut(s)	1196, 1248
Bbv12I	GWGCWC	3 cut(s)	78, 520, 1467
BbvI	GCAGC	9 cut(s)	43, 428, 703, 711, 1065, 1162, 1405, 1592, 1595
BccI	CCATC	3 cut(s)	195, 866, 1313
BciT130I	CCWGG	5 cut(s)	104, 230, 543, 639, 1705
BcnI	CCSGG	1 cut(s)	1596
BcoDI	GTCTC	2 cut(s)	104, 1593
BcuI	ACTAGT	1 cut(s)	212
BfaI	CTAG	6 cut(s)	168, 213, 867, 1061, 1443, 1677
BfmI	CTRYAG	2 cut(s)	717, 1286
BfrI	CTTAAG	1 cut(s)	221
BglII	AGATCT	1 cut(s)	1312
BisI	GCNGC	9 cut(s)	57, 417, 717, 725, 1079, 1176, 1419, 1581, 1584
BlnI	CCTAGG	1 cut(s)	167
BlsI	GCNGC	9 cut(s)	58, 418, 718, 726, 1080, 1177, 1420, 1582, 1585
BmcAI	AGTACT	1 cut(s)	844
Bme1390I	CCNGG	6 cut(s)	104, 230, 543, 639, 1596, 1705
Bme18I	GGWCC	3 cut(s)	695, 1487, 1701
BmgT120I	GGNCC	3 cut(s)	695, 1487, 1701
BmiI	GGNNCC	2 cut(s)	1198, 1250
BmrFI	CCNGG	6 cut(s)	104, 230, 543, 639, 1596, 1705
BmrI	ACTGGG	1 cut(s)	1612
BmsI	GCATC	5 cut(s)	223, 403, 574, 1291, 1486
BmuI	ACTGGG	1 cut(s)	1612
BplI	GAGNNNNNCTC	2 cut(s)	944, 976
BpmI	CTGGAG	1 cut(s)	825
Bpu10I	CCTNAGC	1 cut(s)	1410
Bpu14I	TTCGAA	1 cut(s)	349
BpuEI	CTTGAG	1 cut(s)	1356
BpuMI	CCSGG	1 cut(s)	1596
Bsa29I	ATCGAT	1 cut(s)	879
BsaAI	YACGTR	1 cut(s)	599
BsaBI	GATNNNNATC	1 cut(s)	153
BsaI	GGTCTC	1 cut(s)	104
BsaJI	CCNNGG	5 cut(s)	167, 541, 1344, 1490, 1611
BsaWI	WCCGGW	1 cut(s)	1455
BsaXI	ACNNNNNCTCC	4 cut(s)	507, 537, 1590, 1620
Bsc4I	CCNNNNNNNGG	3 cut(s)	1258, 1568, 1602
Bse1I	ACTGG	5 cut(s)	706, 997, 1010, 1607, 1703
Bse8I	GATNNNNATC	1 cut(s)	153
BseBI	CCWGG	5 cut(s)	104, 230, 543, 639, 1705
BseCI	ATCGAT	1 cut(s)	879
BseDI	CCNNGG	5 cut(s)	167, 541, 1344, 1490, 1611
BseGI	GGATG	6 cut(s)	206, 238, 899, 1097, 1297, 1477
BseJI	GATNNNNATC	1 cut(s)	153
BseLI	CCNNNNNNNGG	3 cut(s)	1258, 1568, 1602
BseMII	CTCAG	1 cut(s)	1424
BseNI	ACTGG	5 cut(s)	706, 997, 1010, 1607, 1703
BseRI	GAGGAG	2 cut(s)	676, 705
BseXI	GCAGC	9 cut(s)	43, 428, 703, 711, 1065, 1162, 1405, 1592, 1595
BshFI	GGCC	1 cut(s)	48
BshNI	GGYRCC	2 cut(s)	1196, 1248
BshVI	ATCGAT	1 cut(s)	879
BsiHKAI	GWGCWC	3 cut(s)	78, 520, 1467
BsiSI	CCGG	2 cut(s)	1456, 1596
BslFI	GGGAC	4 cut(s)	208, 328, 1015, 1034
BslI	CCNNNNNNNGG	3 cut(s)	1258, 1568, 1602
BsmAI	GTCTC	2 cut(s)	104, 1593
BsmFI	GGGAC	4 cut(s)	208, 328, 1015, 1034
BsmI	GAATGC	1 cut(s)	404
BsnI	GGCC	1 cut(s)	48
Bso31I	GGTCTC	1 cut(s)	104
Bsp119I	TTCGAA	1 cut(s)	349
Bsp1286I	GDGCHC	3 cut(s)	78, 520, 1467
Bsp143I	GATC	6 cut(s)	6, 148, 378, 775, 808, 1312
Bsp19I	CCATGG	1 cut(s)	1611
BspACI	CCGC	5 cut(s)	93, 439, 658, 1178, 1586
BspANI	GGCC	1 cut(s)	48
BspCNI	CTCAG	1 cut(s)	1423
BspDI	ATCGAT	1 cut(s)	879
BspLI	GGNNCC	2 cut(s)	1198, 1250
BspMAI	CTGCAG	2 cut(s)	721, 1290
BspPI	GGATC	2 cut(s)	143, 783
BspQI	GCTCTTC	1 cut(s)	648
BspT104I	TTCGAA	1 cut(s)	349
BspT107I	GGYRCC	2 cut(s)	1196, 1248
BspTI	CTTAAG	1 cut(s)	221
BspTNI	GGTCTC	1 cut(s)	104
BsrBI	CCGCTC	1 cut(s)	658
BsrI	ACTGG	5 cut(s)	706, 997, 1010, 1607, 1703
BssECI	CCNNGG	5 cut(s)	167, 541, 1344, 1490, 1611
BssMI	GATC	6 cut(s)	6, 148, 378, 775, 808, 1312
BssT1I	CCWWGG	2 cut(s)	167, 1611
Bst2UI	CCWGG	5 cut(s)	104, 230, 543, 639, 1705
Bst4CI	ACNGT	6 cut(s)	319, 560, 793, 1207, 1345, 1629
Bst6I	CTCTTC	2 cut(s)	648, 1385
BstAFI	CTTAAG	1 cut(s)	221
BstBAI	YACGTR	1 cut(s)	599
BstBI	TTCGAA	1 cut(s)	349
BstC8I	GCNNGC	8 cut(s)	441, 1044, 1280, 1401, 1578, 1654, 1658, 1670
BstDEI	CTNAG	2 cut(s)	644, 1410
BstDSI	CCRYGG	2 cut(s)	1344, 1611
BstF5I	GGATG	6 cut(s)	206, 238, 899, 1097, 1297, 1477
BstHHI	GCGC	1 cut(s)	1423
BstKTI	GATC	6 cut(s)	9, 151, 381, 778, 811, 1315
BstMAI	GTCTC	2 cut(s)	104, 1593
BstMBI	GATC	6 cut(s)	6, 148, 378, 775, 808, 1312
BstMWI	GCNNNNNNNGC	5 cut(s)	35, 73, 515, 1415, 1549
BstNI	CCWGG	5 cut(s)	104, 230, 543, 639, 1705
BstNSI	RCATGY	2 cut(s)	312, 826
BstSCI	CCNGG	6 cut(s)	102, 228, 541, 637, 1594, 1703
BstSFI	CTRYAG	2 cut(s)	717, 1286
BstSNI	TACGTA	1 cut(s)	599
BstV1I	GCAGC	9 cut(s)	43, 428, 703, 711, 1065, 1162, 1405, 1592, 1595
BstX2I	RGATCY	2 cut(s)	775, 1312
BstXI	CCANNNNNNTGG	1 cut(s)	70
BstYI	RGATCY	2 cut(s)	775, 1312
Bsu15I	ATCGAT	1 cut(s)	879
BsuRI	GGCC	1 cut(s)	48
BsuTUI	ATCGAT	1 cut(s)	879
BtgI	CCRYGG	2 cut(s)	1344, 1611
BtsCI	GGATG	6 cut(s)	206, 238, 899, 1097, 1297, 1477
BtsI	GCAGTG	1 cut(s)	1465
BtsIMutI	CAGTG	3 cut(s)	913, 1465, 1634
Cac8I	GCNNGC	8 cut(s)	441, 1044, 1280, 1401, 1578, 1654, 1658, 1670
CfoI	GCGC	1 cut(s)	1423
Cfr13I	GGNCC	3 cut(s)	695, 1487, 1701
ClaI	ATCGAT	1 cut(s)	879
CseI	GACGC	1 cut(s)	391
Csp6I	GTAC	8 cut(s)	81, 210, 226, 632, 843, 1197, 1249, 1537
CspCI	CAANNNNNGTGG	2 cut(s)	957, 992
CviAII	CATG	7 cut(s)	309, 728, 823, 971, 1147, 1517, 1612
CviQI	GTAC	8 cut(s)	81, 210, 226, 632, 843, 1197, 1249, 1537
DdeI	CTNAG	2 cut(s)	644, 1410
DpnI	GATC	6 cut(s)	8, 150, 380, 777, 810, 1314
DpnII	GATC	6 cut(s)	6, 148, 378, 775, 808, 1312
DraI	TTTAAA	4 cut(s)	1171, 1272, 1357, 1501
DraIII	CACNNNGTG	1 cut(s)	1629
DrdI	GACNNNNNNGTC	2 cut(s)	10, 500
DseDI	GACNNNNNNGTC	2 cut(s)	10, 500
EaeI	YGGCCR	1 cut(s)	46
Eam1104I	CTCTTC	2 cut(s)	648, 1385
EarI	CTCTTC	2 cut(s)	648, 1385
Eco105I	TACGTA	1 cut(s)	599
Eco130I	CCWWGG	2 cut(s)	167, 1611
Eco31I	GGTCTC	1 cut(s)	104
Eco32I	GATATC	1 cut(s)	1156
Eco47I	GGWCC	3 cut(s)	695, 1487, 1701
Eco57I	CTGAAG	3 cut(s)	222, 590, 1503
EcoO109I	RGGNCCY	1 cut(s)	695
EcoRII	CCWGG	5 cut(s)	102, 228, 541, 637, 1703
EcoRV	GATATC	1 cut(s)	1156
EcoT14I	CCWWGG	2 cut(s)	167, 1611
ErhI	CCWWGG	2 cut(s)	167, 1611
FaeI	CATG	7 cut(s)	312, 731, 826, 974, 1150, 1520, 1615
FaqI	GGGAC	4 cut(s)	208, 328, 1015, 1034
FatI	CATG	7 cut(s)	308, 727, 822, 970, 1146, 1516, 1611
FauI	CCCGC	1 cut(s)	432
FblI	GTMKAC	1 cut(s)	989
Fnu4HI	GCNGC	9 cut(s)	57, 417, 717, 725, 1079, 1176, 1419, 1581, 1584
FokI	GGATG	6 cut(s)	213, 245, 906, 1104, 1304, 1464
Fsp4HI	GCNGC	9 cut(s)	57, 417, 717, 725, 1079, 1176, 1419, 1581, 1584
FspBI	CTAG	6 cut(s)	168, 213, 867, 1061, 1443, 1677
FspI	TGCGCA	1 cut(s)	1422
GlaI	GCGC	1 cut(s)	1422
GluI	GCNGC	9 cut(s)	57, 417, 717, 725, 1079, 1176, 1419, 1581, 1584
GsuI	CTGGAG	1 cut(s)	825
HaeIII	GGCC	1 cut(s)	48
HapII	CCGG	2 cut(s)	1456, 1596
HgaI	GACGC	1 cut(s)	391
HhaI	GCGC	1 cut(s)	1423
Hin1II	CATG	7 cut(s)	312, 731, 826, 974, 1150, 1520, 1615
Hin6I	GCGC	1 cut(s)	1421
HinP1I	GCGC	1 cut(s)	1421
HincII	GTYRAC	2 cut(s)	1136, 1231
HindII	GTYRAC	2 cut(s)	1136, 1231
HindIII	AAGCTT	1 cut(s)	65
HinfI	GANTC	6 cut(s)	116, 1003, 1379, 1393, 1710, 1727
HpaII	CCGG	2 cut(s)	1456, 1596
HphI	GGTGA	5 cut(s)	13, 255, 949, 1127, 1153
Hpy166II	GTNNAC	6 cut(s)	285, 526, 864, 990, 1136, 1231
Hpy188I	TCNGA	4 cut(s)	570, 916, 1491, 1696
Hpy188III	TCNNGA	9 cut(s)	33, 406, 804, 817, 928, 1220, 1316, 1481, 1720
Hpy8I	GTNNAC	6 cut(s)	285, 526, 864, 990, 1136, 1231
HpyAV	CCTTC	6 cut(s)	25, 565, 928, 940, 1327, 1478
HpyCH4III	ACNGT	6 cut(s)	319, 560, 793, 1207, 1345, 1629
HpyCH4IV	ACGT	4 cut(s)	129, 598, 1054, 1138
HpyCH4V	TGCA	9 cut(s)	113, 416, 719, 727, 1030, 1288, 1403, 1470, 1652
HpyF10VI	GCNNNNNNNGC	5 cut(s)	35, 73, 515, 1415, 1549
HpyF3I	CTNAG	2 cut(s)	644, 1410
HpySE526I	ACGT	4 cut(s)	129, 598, 1054, 1138
Hsp92II	CATG	7 cut(s)	312, 731, 826, 974, 1150, 1520, 1615
HspAI	GCGC	1 cut(s)	1421
KpnI	GGTACC	2 cut(s)	1200, 1252
Kzo9I	GATC	6 cut(s)	6, 148, 378, 775, 808, 1312
LguI	GCTCTTC	1 cut(s)	648
LmnI	GCTCC	3 cut(s)	515, 1564, 1588
Lsp1109I	GCAGC	9 cut(s)	43, 428, 703, 711, 1065, 1162, 1405, 1592, 1595
LweI	GCATC	5 cut(s)	223, 403, 574, 1291, 1486
MaeI	CTAG	6 cut(s)	168, 213, 867, 1061, 1443, 1677
MaeII	ACGT	4 cut(s)	129, 598, 1054, 1138
MaeIII	GTNAC	5 cut(s)	294, 304, 1063, 1294, 1339
MalI	GATC	6 cut(s)	8, 150, 380, 777, 810, 1314
MbiI	CCGCTC	1 cut(s)	658
MboI	GATC	6 cut(s)	6, 148, 378, 775, 808, 1312
MflI	RGATCY	2 cut(s)	775, 1312
MhlI	GDGCHC	3 cut(s)	78, 520, 1467
MlsI	TGGCCA	1 cut(s)	48
MluNI	TGGCCA	1 cut(s)	48
MlyI	GAGTC	2 cut(s)	997, 1402
MmeI	TCCRAC	2 cut(s)	150, 667
MnlI	CCTC	8 cut(s)	70, 89, 654, 683, 686, 946, 1011, 1485
Mox20I	TGGCCA	1 cut(s)	48
MroXI	GAANNNNTTC	1 cut(s)	345
MscI	TGGCCA	1 cut(s)	48
MseI	TTAA	9 cut(s)	222, 534, 771, 1170, 1202, 1271, 1356, 1452, 1500
Msp20I	TGGCCA	1 cut(s)	48
MspA1I	CMGCKG	1 cut(s)	1586
MspCI	CTTAAG	1 cut(s)	221
MspI	CCGG	2 cut(s)	1456, 1596
MspR9I	CCNGG	6 cut(s)	104, 230, 543, 639, 1596, 1705
Mva1269I	GAATGC	1 cut(s)	404
MvaI	CCWGG	5 cut(s)	104, 230, 543, 639, 1705
MwoI	GCNNNNNNNGC	5 cut(s)	35, 73, 515, 1415, 1549
NciI	CCSGG	1 cut(s)	1596
NcoI	CCATGG	1 cut(s)	1611
NdeII	GATC	6 cut(s)	6, 148, 378, 775, 808, 1312
NlaIII	CATG	7 cut(s)	312, 731, 826, 974, 1150, 1520, 1615
NlaIV	GGNNCC	2 cut(s)	1198, 1250
NmeAIII	GCCGAG	1 cut(s)	877
NmuCI	GTSAC	3 cut(s)	304, 1294, 1339
NsbI	TGCGCA	1 cut(s)	1422
NspI	RCATGY	2 cut(s)	312, 826
NspV	TTCGAA	1 cut(s)	349
PciI	ACATGT	2 cut(s)	308, 822
PciSI	GCTCTTC	1 cut(s)	648
PctI	GAATGC	1 cut(s)	404
PdmI	GAANNNNTTC	1 cut(s)	345
PfeI	GAWTC	4 cut(s)	116, 1379, 1710, 1727
PkrI	GCNGC	9 cut(s)	58, 418, 718, 726, 1080, 1177, 1420, 1582, 1585
PleI	GAGTC	2 cut(s)	997, 1401
PpsI	GAGTC	2 cut(s)	997, 1401
Ppu21I	YACGTR	1 cut(s)	599
PpuMI	RGGWCCY	1 cut(s)	695
PscI	ACATGT	2 cut(s)	308, 822
Psp1406I	AACGTT	1 cut(s)	129
Psp5II	RGGWCCY	1 cut(s)	695
Psp6I	CCWGG	5 cut(s)	102, 228, 541, 637, 1703
PspGI	CCWGG	5 cut(s)	102, 228, 541, 637, 1703
PspN4I	GGNNCC	2 cut(s)	1198, 1250
PspPI	GGNCC	3 cut(s)	695, 1487, 1701
PspPPI	RGGWCCY	1 cut(s)	695
PstI	CTGCAG	2 cut(s)	721, 1290
PsuI	RGATCY	2 cut(s)	775, 1312
RsaI	GTAC	8 cut(s)	82, 211, 227, 633, 844, 1198, 1250, 1538
RsaNI	GTAC	8 cut(s)	81, 210, 226, 632, 843, 1197, 1249, 1537
SapI	GCTCTTC	1 cut(s)	648
SaqAI	TTAA	9 cut(s)	222, 534, 771, 1170, 1202, 1271, 1356, 1452, 1500
SatI	GCNGC	9 cut(s)	57, 417, 717, 725, 1079, 1176, 1419, 1581, 1584
Sau3AI	GATC	6 cut(s)	6, 148, 378, 775, 808, 1312
Sau96I	GGNCC	3 cut(s)	695, 1487, 1701
ScaI	AGTACT	1 cut(s)	844
SchI	GAGTC	2 cut(s)	997, 1402
ScrFI	CCNGG	6 cut(s)	104, 230, 543, 639, 1596, 1705
SduI	GDGCHC	3 cut(s)	78, 520, 1467
SfaNI	GCATC	5 cut(s)	223, 403, 574, 1291, 1486
SfcI	CTRYAG	2 cut(s)	717, 1286
SfuI	TTCGAA	1 cut(s)	349
SinI	GGWCC	3 cut(s)	695, 1487, 1701
SmiI	ATTTAAAT	1 cut(s)	1357
SmlI	CTYRAG	2 cut(s)	221, 1371
SmoI	CTYRAG	2 cut(s)	221, 1371
SnaBI	TACGTA	1 cut(s)	599
SpeI	ACTAGT	1 cut(s)	212
SsiI	CCGC	5 cut(s)	93, 439, 658, 1178, 1586
SspMI	CTAG	6 cut(s)	168, 213, 867, 1061, 1443, 1677
StyD4I	CCNGG	6 cut(s)	102, 228, 541, 637, 1594, 1703
StyI	CCWWGG	2 cut(s)	167, 1611
SwaI	ATTTAAAT	1 cut(s)	1357
TaaI	ACNGT	6 cut(s)	319, 560, 793, 1207, 1345, 1629
TaiI	ACGT	4 cut(s)	132, 601, 1057, 1141
TaqI	TCGA	5 cut(s)	349, 879, 944, 1219, 1317
TatI	WGTACW	3 cut(s)	80, 631, 842
TfiI	GAWTC	4 cut(s)	116, 1379, 1710, 1727
Tru1I	TTAA	9 cut(s)	222, 534, 771, 1170, 1202, 1271, 1356, 1452, 1500
Tru9I	TTAA	9 cut(s)	222, 534, 771, 1170, 1202, 1271, 1356, 1452, 1500
TscAI	CASTG	3 cut(s)	913, 1472, 1634
TseFI	GTSAC	3 cut(s)	304, 1294, 1339
TseI	GCWGC	9 cut(s)	56, 416, 716, 724, 1078, 1175, 1418, 1580, 1583
Tsp45I	GTSAC	3 cut(s)	304, 1294, 1339
TspDTI	ATGAA	5 cut(s)	662, 947, 1227, 1403, 1702
TspGWI	ACGGA	1 cut(s)	1551
TspRI	CASTG	3 cut(s)	913, 1472, 1634
Vha464I	CTTAAG	1 cut(s)	221
VpaK11BI	GGWCC	3 cut(s)	695, 1487, 1701
XapI	RAATTY	5 cut(s)	383, 476, 710, 1214, 1352
XceI	RCATGY	2 cut(s)	312, 826
XmaJI	CCTAGG	1 cut(s)	167
XmiI	GTMKAC	1 cut(s)	989
XmnI	GAANNNNTTC	1 cut(s)	345
XspI	CTAG	6 cut(s)	168, 213, 867, 1061, 1443, 1677
ZrmI	AGTACT	1 cut(s)	844

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.