Rorug02G0493000

External alternative NAD(P)H-ubiquinone oxidoreductase B1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008977
Physical Location & Seq
Reverse (-)
62180107 .. 62180913
807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0493000.1

Sequence Viewer

Length: 348 bp
ATGAGTTCCAGCACTAATTCCGCAGCAGCGTACACCGCTCCGGCTTCACAACAGGGACCTTACGTCCAGGCTCCGCCTCCTGCCGGCTATCCCACCATGAGAGACGACGCCGACTTGCCCCAGAGTCATGGTCGTGTGGAAACCAAGTCCAAGGGTGATGGCTTCTGGAAGGGCTGCTGTGCTGCCTTGTGTTGCTGTTATCTAGTGGAGCTCCACCTTGATGATTCTCGACCCATCTTCAGAGACACAAGCAAGTCCACCGGTGCCCTCTTGACCGGTCTCCAGCTGGGCAAAGGTGAGGTGGTGACTGACATGGAGAAAAAATGGCTTCCACCGGTGGAGAAGTGA

Protein Analysis

115

Amino Acids

12.33

Weight (kDa)

6.06

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYSTM PF12734 21 - 70 2.9e-09 Cysteine-rich TM module stress tolerance
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 263
AccBSI CCGCTC 1 cut(s) 38
AciI CCGC 3 cut(s) 21, 36, 74
AcuI CTGAAG 1 cut(s) 223
AcyI GRCGYC 1 cut(s) 108
AfaI GTAC 1 cut(s) 32
AfiI CCNNNNNNNGG 1 cut(s) 83
AgeI ACCGGT 3 cut(s) 260, 275, 334
AhdI GACNNNNNGTC 1 cut(s) 62
AjnI CCWGG 1 cut(s) 66
AluBI AGCT 2 cut(s) 211, 286
AluI AGCT 2 cut(s) 211, 286
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 3 cut(s) 96, 237, 284
ApeKI GCWGC 4 cut(s) 23, 26, 174, 182
AsiGI ACCGGT 3 cut(s) 260, 275, 334
AspS9I GGNCC 1 cut(s) 56
AsuHPI GGTGA 3 cut(s) 167, 308, 316
AvaII GGWCC 1 cut(s) 56
BaeGI GKGCMC 1 cut(s) 268
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 4 cut(s) 35, 38, 161, 169
BccI CCATC 2 cut(s) 152, 242
BciT130I CCWGG 1 cut(s) 68
BcoDI GTCTC 3 cut(s) 96, 237, 284
BfaI CTAG 1 cut(s) 203
BisI GCNGC 4 cut(s) 24, 27, 175, 183
BlsI GCNGC 4 cut(s) 25, 28, 176, 184
Bme1390I CCNGG 1 cut(s) 68
Bme18I GGWCC 1 cut(s) 56
BmeRI GACNNNNNGTC 1 cut(s) 62
BmgT120I GGNCC 1 cut(s) 56
BmiI GGNNCC 3 cut(s) 57, 72, 265
BmrFI CCNGG 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 266
BsaHI GRCGYC 1 cut(s) 108
BsaI GGTCTC 1 cut(s) 284
BsaJI CCNNGG 1 cut(s) 150
BsaWI WCCGGW 3 cut(s) 260, 275, 334
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse118I RCCGGY 4 cut(s) 83, 260, 275, 334
BseBI CCWGG 1 cut(s) 68
BseDI CCNNGG 1 cut(s) 150
BseLI CCNNNNNNNGG 1 cut(s) 83
BseSI GKGCMC 1 cut(s) 268
BseXI GCAGC 4 cut(s) 35, 38, 161, 169
BseYI CCCAGC 1 cut(s) 286
BshNI GGYRCC 1 cut(s) 263
BshTI ACCGGT 3 cut(s) 260, 275, 334
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 5 cut(s) 41, 84, 261, 276, 335
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 3 cut(s) 96, 237, 284
BsmBI CGTCTC 1 cut(s) 96
BsmFI GGGAC 1 cut(s) 69
Bso31I GGTCTC 1 cut(s) 284
Bsp1286I GDGCHC 2 cut(s) 213, 268
BspACI CCGC 3 cut(s) 21, 36, 74
BspLI GGNNCC 3 cut(s) 57, 72, 265
BspT107I GGYRCC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 284
BsrBI CCGCTC 1 cut(s) 38
BsrFI RCCGGY 4 cut(s) 83, 260, 275, 334
BssAI RCCGGY 4 cut(s) 83, 260, 275, 334
BssECI CCNNGG 1 cut(s) 150
BssNI GRCGYC 1 cut(s) 108
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 68
BstACI GRCGYC 1 cut(s) 108
BstC8I GCNNGC 1 cut(s) 85
BstMAI GTCTC 3 cut(s) 96, 237, 284
BstMWI GCNNNNNNNGC 1 cut(s) 35
BstNI CCWGG 1 cut(s) 68
BstSCI CCNGG 1 cut(s) 66
BstSLI GKGCMC 1 cut(s) 268
BstV1I GCAGC 4 cut(s) 35, 38, 161, 169
BstXI CCANNNNNNTGG 1 cut(s) 128
Cac8I GCNNGC 1 cut(s) 85
Cfr10I RCCGGY 4 cut(s) 83, 260, 275, 334
Cfr13I GGNCC 1 cut(s) 56
CseI GACGC 1 cut(s) 116
Csp6I GTAC 1 cut(s) 31
CspAI ACCGGT 3 cut(s) 260, 275, 334
CviAII CATG 3 cut(s) 97, 128, 313
CviJI RGCY 8 cut(s) 44, 71, 87, 162, 174, 211, 286, 328
CviKI_1 RGCY 8 cut(s) 44, 71, 87, 162, 174, 211, 286, 328
CviQI GTAC 1 cut(s) 31
DriI GACNNNNNGTC 1 cut(s) 62
Eam1105I GACNNNNNGTC 1 cut(s) 62
EciI GGCGGA 1 cut(s) 63
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 213
Eco31I GGTCTC 1 cut(s) 284
Eco47I GGWCC 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 223
EcoICRI GAGCTC 1 cut(s) 211
EcoO109I RGGNCCY 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 66
EcoT14I CCWWGG 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 150
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 3 cut(s) 100, 131, 316
FaiI YATR 3 cut(s) 98, 129, 314
FaqI GGGAC 1 cut(s) 69
FatI CATG 3 cut(s) 96, 127, 312
Fnu4HI GCNGC 4 cut(s) 24, 27, 175, 183
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 4 cut(s) 24, 27, 175, 183
FspBI CTAG 1 cut(s) 203
GluI GCNGC 4 cut(s) 24, 27, 175, 183
GsaI CCCAGC 1 cut(s) 290
GsuI CTGGAG 1 cut(s) 266
HapII CCGG 5 cut(s) 41, 84, 261, 276, 335
HgaI GACGC 1 cut(s) 116
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 3 cut(s) 100, 131, 316
HinfI GANTC 2 cut(s) 124, 224
HpaII CCGG 5 cut(s) 41, 84, 261, 276, 335
HphI GGTGA 3 cut(s) 167, 308, 316
Hpy166II GTNNAC 2 cut(s) 33, 258
Hpy188I TCNGA 1 cut(s) 242
Hpy188III TCNNGA 3 cut(s) 166, 228, 271
Hpy8I GTNNAC 2 cut(s) 33, 258
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 1 cut(s) 163
HpyCH4IV ACGT 1 cut(s) 63
HpyF10VI GCNNNNNNNGC 1 cut(s) 35
HpySE526I ACGT 1 cut(s) 63
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 3 cut(s) 100, 131, 316
KroI GCCGGC 1 cut(s) 83
KroNI GCCGGC 1 cut(s) 85
LmnI GCTCC 4 cut(s) 43, 76, 208, 216
Lsp1109I GCAGC 4 cut(s) 35, 38, 161, 169
MaeI CTAG 1 cut(s) 203
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 1 cut(s) 304
MbiI CCGCTC 1 cut(s) 38
MboII GAAGA 1 cut(s) 229
MhlI GDGCHC 2 cut(s) 213, 268
MluCI AATT 1 cut(s) 16
MlyI GAGTC 1 cut(s) 133
MnlI CCTC 3 cut(s) 87, 278, 292
MroNI GCCGGC 1 cut(s) 83
MslI CAYNNNNRTG 2 cut(s) 132, 219
MspA1I CMGCKG 1 cut(s) 286
MspI CCGG 5 cut(s) 41, 84, 261, 276, 335
MspR9I CCNGG 1 cut(s) 68
MvaI CCWGG 1 cut(s) 68
MwoI GCNNNNNNNGC 1 cut(s) 35
NaeI GCCGGC 1 cut(s) 85
NgoMIV GCCGGC 1 cut(s) 83
NlaIII CATG 3 cut(s) 100, 131, 316
NlaIV GGNNCC 3 cut(s) 57, 72, 265
NmuCI GTSAC 1 cut(s) 304
PdiI GCCGGC 1 cut(s) 85
PfeI GAWTC 1 cut(s) 224
PinAI ACCGGT 3 cut(s) 260, 275, 334
PkrI GCNGC 4 cut(s) 25, 28, 176, 184
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
PpuMI RGGWCCY 1 cut(s) 56
Psp124BI GAGCTC 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 56
Psp6I CCWGG 1 cut(s) 66
PspFI CCCAGC 1 cut(s) 286
PspGI CCWGG 1 cut(s) 66
PspN4I GGNNCC 3 cut(s) 57, 72, 265
PspPI GGNCC 1 cut(s) 56
PspPPI RGGWCCY 1 cut(s) 56
PvuII CAGCTG 1 cut(s) 286
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
RseI CAYNNNNRTG 2 cut(s) 132, 219
SacI GAGCTC 1 cut(s) 213
SatI GCNGC 4 cut(s) 24, 27, 175, 183
Sau96I GGNCC 1 cut(s) 56
SchI GAGTC 1 cut(s) 133
ScrFI CCNGG 1 cut(s) 68
SduI GDGCHC 2 cut(s) 213, 268
SetI ASST 7 cut(s) 61, 66, 213, 219, 288, 298, 303
SgrAI CRCCGGYG 2 cut(s) 260, 334
SinI GGWCC 1 cut(s) 56
SmiMI CAYNNNNRTG 2 cut(s) 132, 219
Sse9I AATT 1 cut(s) 16
SsiI CCGC 3 cut(s) 21, 36, 74
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 1 cut(s) 213
StyD4I CCNGG 1 cut(s) 66
StyI CCWWGG 1 cut(s) 150
TaiI ACGT 1 cut(s) 66
TaqI TCGA 1 cut(s) 229
TasI AATT 1 cut(s) 16
TfiI GAWTC 1 cut(s) 224
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 4 cut(s) 23, 26, 174, 182
Tsp45I GTSAC 1 cut(s) 304
VpaK11BI GGWCC 1 cut(s) 56
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.