MD09G1108900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006068
Physical Location & Seq
Forward (+)
8065563 .. 8065943
381 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1108900.v1.1.491

Sequence Viewer

Length: 381 bp
ATGAAACGCCGATCTAGACTCCACCGCCGGTCCTCCCCCAACCGGTCGGCCGAGCCATTTCTCAAATACCTAAAGCCCGGCGCGCTCGCCCAAATCCGAGACTCCCGAATCAGCAGGGCCAGATCGCACCAGCTCAACTGGCTCTCTCAGATCTGTGTATCGCCGCCGTTATCTCCGGGCCCCGACGCGGGTCTTCCCCAGGCCAACGTGATGGAGGGATTCCCTTGCTTCTCGGGTCGGATCTACGGCCCCAGATGTCCCCAGCGGAAGAAGCTTGCGGCGTCCAAGTCGGTTATGTTCCTCAGTCCCTCGAGCCCGGTTCACGACTCTCCCGATCCGATAATCGATCTGTTTAGTACGGACATTCTTGCTGCACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

127

Amino Acids

13.97

Weight (kDa)

10.64

Isoelectric Point (pI)

78.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 83, 188
AciI CCGC 5 cut(s) 25, 164, 188, 265, 278
AclWI GGATC 2 cut(s) 248, 329
AcoI YGGCCR 1 cut(s) 48
AcyI GRCGYC 1 cut(s) 281
AfaI GTAC 1 cut(s) 358
AfiI CCNNNNNNNGG 3 cut(s) 42, 187, 188
AgeI ACCGGT 1 cut(s) 42
AjnI CCWGG 1 cut(s) 198
AluBI AGCT 2 cut(s) 133, 274
AluI AGCT 2 cut(s) 133, 274
Alw26I GTCTC 1 cut(s) 93
AlwI GGATC 2 cut(s) 248, 329
Ama87I CYCGRG 2 cut(s) 232, 310
AoxI GGCC 5 cut(s) 48, 117, 178, 201, 247
ApaI GGGCCC 1 cut(s) 182
ApeKI GCWGC 1 cut(s) 371
AsiGI ACCGGT 1 cut(s) 42
AspLEI GCGC 2 cut(s) 83, 85
AspS9I GGNCC 5 cut(s) 30, 117, 178, 179, 248
AsuC2I CCSGG 3 cut(s) 78, 177, 317
AvaI CYCGRG 2 cut(s) 232, 310
AvaII GGWCC 1 cut(s) 30
BaeGI GKGCMC 1 cut(s) 182
BanII GRGCYC 2 cut(s) 182, 317
BbsI GAAGAC 1 cut(s) 185
BbvI GCAGC 1 cut(s) 358
BccI CCATC 1 cut(s) 205
BceAI ACGGC 2 cut(s) 151, 262
BciT130I CCWGG 1 cut(s) 200
BcnI CCSGG 3 cut(s) 78, 177, 317
BcoDI GTCTC 1 cut(s) 93
BfaI CTAG 1 cut(s) 15
BglII AGATCT 1 cut(s) 150
BisI GCNGC 3 cut(s) 164, 279, 372
BlsI GCNGC 3 cut(s) 165, 280, 373
Bme1390I CCNGG 4 cut(s) 78, 177, 200, 317
Bme18I GGWCC 1 cut(s) 30
BmeT110I CYCGRG 2 cut(s) 232, 310
BmgT120I GGNCC 5 cut(s) 30, 117, 178, 179, 248
BmiI GGNNCC 3 cut(s) 180, 181, 250
BmrFI CCNGG 4 cut(s) 78, 177, 200, 317
BoxI GACNNNNGTC 1 cut(s) 189
BpiI GAAGAC 1 cut(s) 185
BpuMI CCSGG 3 cut(s) 78, 177, 317
Bsa29I ATCGAT 1 cut(s) 345
BsaHI GRCGYC 1 cut(s) 281
BsaJI CCNNGG 1 cut(s) 198
BsaWI WCCGGW 1 cut(s) 42
Bsc4I CCNNNNNNNGG 3 cut(s) 42, 187, 188
Bse118I RCCGGY 2 cut(s) 27, 42
Bse1I ACTGG 1 cut(s) 143
BseBI CCWGG 1 cut(s) 200
BseCI ATCGAT 1 cut(s) 345
BseDI CCNNGG 1 cut(s) 198
BseLI CCNNNNNNNGG 3 cut(s) 42, 187, 188
BseMII CTCAG 2 cut(s) 161, 316
BseNI ACTGG 1 cut(s) 143
BsePI GCGCGC 1 cut(s) 81
BseSI GKGCMC 1 cut(s) 182
BseX3I CGGCCG 1 cut(s) 48
BseXI GCAGC 1 cut(s) 358
BseYI CCCAGC 1 cut(s) 261
BsgI GTGCAG 1 cut(s) 357
Bsh1236I CGCG 2 cut(s) 83, 188
Bsh1285I CGRYCG 2 cut(s) 47, 51
BshFI GGCC 5 cut(s) 50, 119, 180, 203, 249
BshTI ACCGGT 1 cut(s) 42
BshVI ATCGAT 1 cut(s) 345
BsiEI CGRYCG 2 cut(s) 47, 51
BsiHKCI CYCGRG 2 cut(s) 232, 310
BsiSI CCGG 5 cut(s) 28, 43, 78, 176, 317
BslFI GGGAC 2 cut(s) 243, 291
BslI CCNNNNNNNGG 3 cut(s) 42, 187, 188
BsmAI GTCTC 1 cut(s) 93
BsmFI GGGAC 2 cut(s) 243, 291
BsnI GGCC 5 cut(s) 50, 119, 180, 203, 249
BsoBI CYCGRG 2 cut(s) 232, 310
Bsp120I GGGCCC 1 cut(s) 178
Bsp1286I GDGCHC 2 cut(s) 182, 317
Bsp143I GATC 6 cut(s) 11, 122, 150, 240, 334, 346
BspACI CCGC 5 cut(s) 25, 164, 188, 265, 278
BspANI GGCC 5 cut(s) 50, 119, 180, 203, 249
BspCNI CTCAG 2 cut(s) 160, 315
BspDI ATCGAT 1 cut(s) 345
BspFNI CGCG 2 cut(s) 83, 188
BspLI GGNNCC 3 cut(s) 180, 181, 250
BspPI GGATC 2 cut(s) 248, 329
BsrFI RCCGGY 2 cut(s) 27, 42
BsrI ACTGG 1 cut(s) 143
BssAI RCCGGY 2 cut(s) 27, 42
BssECI CCNNGG 1 cut(s) 198
BssHII GCGCGC 1 cut(s) 81
BssMI GATC 6 cut(s) 11, 122, 150, 240, 334, 346
BssNI GRCGYC 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 200
BstACI GRCGYC 1 cut(s) 281
BstC8I GCNNGC 3 cut(s) 83, 87, 276
BstDEI CTNAG 2 cut(s) 147, 302
BstFNI CGCG 2 cut(s) 83, 188
BstHHI GCGC 2 cut(s) 83, 85
BstKTI GATC 6 cut(s) 14, 125, 153, 243, 337, 349
BstMAI GTCTC 1 cut(s) 93
BstMBI GATC 6 cut(s) 11, 122, 150, 240, 334, 346
BstMCI CGRYCG 2 cut(s) 47, 51
BstMWI GCNNNNNNNGC 3 cut(s) 82, 139, 271
BstNI CCWGG 1 cut(s) 200
BstPAI GACNNNNGTC 1 cut(s) 189
BstSCI CCNGG 4 cut(s) 76, 175, 198, 315
BstSLI GKGCMC 1 cut(s) 182
BstUI CGCG 2 cut(s) 83, 188
BstV1I GCAGC 1 cut(s) 358
BstV2I GAAGAC 1 cut(s) 185
BstX2I RGATCY 2 cut(s) 150, 240
BstXI CCANNNNNNTGG 1 cut(s) 211
BstYI RGATCY 2 cut(s) 150, 240
BstZI CGGCCG 1 cut(s) 48
Bsu15I ATCGAT 1 cut(s) 345
BsuRI GGCC 5 cut(s) 50, 119, 180, 203, 249
BsuTUI ATCGAT 1 cut(s) 345
Cac8I GCNNGC 3 cut(s) 83, 87, 276
CfoI GCGC 2 cut(s) 83, 85
Cfr10I RCCGGY 2 cut(s) 27, 42
Cfr13I GGNCC 5 cut(s) 30, 117, 178, 179, 248
ClaI ATCGAT 1 cut(s) 345
CseI GACGC 2 cut(s) 194, 270
Csp6I GTAC 1 cut(s) 357
CspAI ACCGGT 1 cut(s) 42
CviQI GTAC 1 cut(s) 357
DdeI CTNAG 2 cut(s) 147, 302
DpnI GATC 6 cut(s) 13, 124, 152, 242, 336, 348
DpnII GATC 6 cut(s) 11, 122, 150, 240, 334, 346
EaeI YGGCCR 1 cut(s) 48
EagI CGGCCG 1 cut(s) 48
EclXI CGGCCG 1 cut(s) 48
Eco24I GRGCYC 2 cut(s) 182, 317
Eco47I GGWCC 1 cut(s) 30
Eco52I CGGCCG 1 cut(s) 48
Eco88I CYCGRG 2 cut(s) 232, 310
EcoO109I RGGNCCY 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 198
EcoT38I GRGCYC 2 cut(s) 182, 317
FaiI YATR 1 cut(s) 296
FaqI GGGAC 2 cut(s) 243, 291
FauI CCCGC 1 cut(s) 181
Fnu4HI GCNGC 3 cut(s) 164, 279, 372
FriOI GRGCYC 2 cut(s) 182, 317
Fsp4HI GCNGC 3 cut(s) 164, 279, 372
FspBI CTAG 1 cut(s) 15
GlaI GCGC 2 cut(s) 82, 84
GluI GCNGC 3 cut(s) 164, 279, 372
GsaI CCCAGC 1 cut(s) 265
HaeIII GGCC 5 cut(s) 50, 119, 180, 203, 249
HapII CCGG 5 cut(s) 28, 43, 78, 176, 317
HgaI GACGC 2 cut(s) 194, 270
HhaI GCGC 2 cut(s) 83, 85
Hin1I GRCGYC 1 cut(s) 281
Hin6I GCGC 2 cut(s) 81, 83
HinP1I GCGC 2 cut(s) 81, 83
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 5 cut(s) 18, 101, 108, 219, 326
HpaII CCGG 5 cut(s) 28, 43, 78, 176, 317
Hpy166II GTNNAC 1 cut(s) 322
Hpy188I TCNGA 4 cut(s) 98, 150, 240, 339
Hpy188III TCNNGA 4 cut(s) 15, 105, 323, 332
Hpy8I GTNNAC 1 cut(s) 322
Hpy99I CGWCG 1 cut(s) 188
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 1 cut(s) 374
HpyF10VI GCNNNNNNNGC 3 cut(s) 82, 139, 271
HpyF3I CTNAG 2 cut(s) 147, 302
HpySE526I ACGT 1 cut(s) 207
Hsp92I GRCGYC 1 cut(s) 281
HspAI GCGC 2 cut(s) 81, 83
Kzo9I GATC 6 cut(s) 11, 122, 150, 240, 334, 346
Lsp1109I GCAGC 1 cut(s) 358
MaeI CTAG 1 cut(s) 15
MaeII ACGT 1 cut(s) 207
MalI GATC 6 cut(s) 13, 124, 152, 242, 336, 348
MboI GATC 6 cut(s) 11, 122, 150, 240, 334, 346
MboII GAAGA 2 cut(s) 185, 280
MflI RGATCY 2 cut(s) 150, 240
MhlI GDGCHC 2 cut(s) 182, 317
MlyI GAGTC 3 cut(s) 12, 95, 320
MmeI TCCRAC 1 cut(s) 218
MnlI CCTC 4 cut(s) 43, 208, 311, 319
MspA1I CMGCKG 1 cut(s) 265
MspI CCGG 5 cut(s) 28, 43, 78, 176, 317
MspR9I CCNGG 4 cut(s) 78, 177, 200, 317
MvaI CCWGG 1 cut(s) 200
MvnI CGCG 2 cut(s) 83, 188
MwoI GCNNNNNNNGC 3 cut(s) 82, 139, 271
NciI CCSGG 3 cut(s) 78, 177, 317
NdeII GATC 6 cut(s) 11, 122, 150, 240, 334, 346
NlaIV GGNNCC 3 cut(s) 180, 181, 250
NmeAIII GCCGAG 1 cut(s) 76
PaeR7I CTCGAG 1 cut(s) 310
PauI GCGCGC 1 cut(s) 81
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PfeI GAWTC 2 cut(s) 108, 219
PinAI ACCGGT 1 cut(s) 42
PkrI GCNGC 3 cut(s) 165, 280, 373
PleI GAGTC 3 cut(s) 12, 95, 320
PpsI GAGTC 3 cut(s) 12, 95, 320
PshAI GACNNNNGTC 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 198
PspFI CCCAGC 1 cut(s) 261
PspGI CCWGG 1 cut(s) 198
PspN4I GGNNCC 3 cut(s) 180, 181, 250
PspOMI GGGCCC 1 cut(s) 178
PspPI GGNCC 5 cut(s) 30, 117, 178, 179, 248
PspXI VCTCGAGB 1 cut(s) 310
PsuI RGATCY 2 cut(s) 150, 240
PteI GCGCGC 1 cut(s) 81
RsaI GTAC 1 cut(s) 358
RsaNI GTAC 1 cut(s) 357
SatI GCNGC 3 cut(s) 164, 279, 372
Sau3AI GATC 6 cut(s) 11, 122, 150, 240, 334, 346
Sau96I GGNCC 5 cut(s) 30, 117, 178, 179, 248
SchI GAGTC 3 cut(s) 12, 95, 320
ScrFI CCNGG 4 cut(s) 78, 177, 200, 317
SduI GDGCHC 2 cut(s) 182, 317
SetI ASST 4 cut(s) 72, 135, 210, 276
Sfr274I CTCGAG 1 cut(s) 310
SinI GGWCC 1 cut(s) 30
SlaI CTCGAG 1 cut(s) 310
SmlI CTYRAG 1 cut(s) 310
SmoI CTYRAG 1 cut(s) 310
SsiI CCGC 5 cut(s) 25, 164, 188, 265, 278
SspMI CTAG 1 cut(s) 15
StyD4I CCNGG 4 cut(s) 76, 175, 198, 315
TaiI ACGT 1 cut(s) 210
TaqI TCGA 2 cut(s) 311, 345
TauI GCSGC 2 cut(s) 166, 281
TfiI GAWTC 2 cut(s) 108, 219
TseI GCWGC 1 cut(s) 371
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 374
VpaK11BI GGWCC 1 cut(s) 30
XbaI TCTAGA 1 cut(s) 14
XhoI CTCGAG 1 cut(s) 310
XspI CTAG 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.