Rroxscaffold_3G00269970

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006068
Physical Location & Seq
Forward (+)
62678968 .. 62681836
2869 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00269970.1

Sequence Viewer

Length: 417 bp
ATGAACCGCCGTGCACGAACCACCCACCAGTCCTCTTCGAATTGGTCCGAATTGTTCCTCAAGTACCTAATACCGAGCCCTAGCTCAAATCGGAGACAAGCGAATCAACAACTCCGGATCTCACAGAATCAACCGGATCTCCCATATTCGTTCGACTCGCCGTTGTCTCCCTTCGCCGACTCTTGTCTCCCACAGATCACCGTCGCCGCCGATGGATTCCCTTGCTTCTCCCACGGATCGACAAAGAACGACATCGTCTCGGCCATCGAAGATGGTGGATCCATGCAGAGATATAAGCTCTTGCAGCATATGTCCAAGGAGGAATGCCTGAAGGCGGGGCGAATGCTTCCACTAGCCATTTCAAATGTCGCCACTGACACTGAAAGAGAGGAATGTAAAGCGAGACCAGCTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

15.45

Weight (kDa)

8.41

Isoelectric Point (pI)

79.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 114
AciI CCGC 3 cut(s) 7, 207, 335
AclWI GGATC 5 cut(s) 125, 144, 244, 273, 286
AcoI YGGCCR 1 cut(s) 261
AcuI CTGAAG 1 cut(s) 350
AfaI GTAC 1 cut(s) 65
AfiI CCNNNNNNNGG 1 cut(s) 334
AgsI TTSAA 1 cut(s) 363
AluBI AGCT 3 cut(s) 84, 298, 410
AluI AGCT 3 cut(s) 84, 298, 410
Alw21I GWGCWC 1 cut(s) 16
Alw26I GTCTC 5 cut(s) 88, 171, 191, 262, 397
Alw44I GTGCAC 1 cut(s) 12
AlwI GGATC 5 cut(s) 125, 144, 244, 273, 286
Aor13HI TCCGGA 1 cut(s) 114
AoxI GGCC 1 cut(s) 261
ApaLI GTGCAC 1 cut(s) 12
ApeKI GCWGC 1 cut(s) 304
AspS9I GGNCC 1 cut(s) 45
AsuHPI GGTGA 1 cut(s) 190
AsuII TTCGAA 1 cut(s) 38
AvaII GGWCC 1 cut(s) 45
BaeGI GKGCMC 1 cut(s) 16
BamHI GGATCC 1 cut(s) 278
BanII GRGCYC 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 16
BbvI GCAGC 1 cut(s) 316
BccI CCATC 3 cut(s) 206, 266, 272
BceAI ACGGC 1 cut(s) 145
BcoDI GTCTC 5 cut(s) 88, 171, 191, 262, 397
BfaI CTAG 2 cut(s) 81, 353
BisI GCNGC 2 cut(s) 207, 305
BlsI GCNGC 2 cut(s) 208, 306
Bme18I GGWCC 1 cut(s) 45
BmgT120I GGNCC 1 cut(s) 45
BmiI GGNNCC 1 cut(s) 280
BoxI GACNNNNGTC 1 cut(s) 183
BplI GAGNNNNNCTC 1 cut(s) 394
Bpu14I TTCGAA 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 44
BsaI GGTCTC 1 cut(s) 397
BsaJI CCNNGG 2 cut(s) 232, 315
BsaWI WCCGGW 2 cut(s) 114, 133
BsaXI ACNNNNNCTCC 2 cut(s) 123, 153
Bsc4I CCNNNNNNNGG 1 cut(s) 334
Bse1I ACTGG 1 cut(s) 28
BseAI TCCGGA 1 cut(s) 114
BseDI CCNNGG 2 cut(s) 232, 315
BseLI CCNNNNNNNGG 1 cut(s) 334
BseNI ACTGG 1 cut(s) 28
BseSI GKGCMC 1 cut(s) 16
BseXI GCAGC 1 cut(s) 316
BshFI GGCC 1 cut(s) 263
BsiHKAI GWGCWC 1 cut(s) 16
BsiSI CCGG 2 cut(s) 115, 134
BslI CCNNNNNNNGG 1 cut(s) 334
BsmAI GTCTC 5 cut(s) 88, 171, 191, 262, 397
BsmBI CGTCTC 1 cut(s) 262
BsmI GAATGC 2 cut(s) 329, 348
BsnI GGCC 1 cut(s) 263
Bso31I GGTCTC 1 cut(s) 397
Bsp119I TTCGAA 1 cut(s) 38
Bsp1286I GDGCHC 2 cut(s) 16, 80
Bsp13I TCCGGA 1 cut(s) 114
Bsp143I GATC 5 cut(s) 117, 136, 195, 236, 278
BspACI CCGC 3 cut(s) 7, 207, 335
BspANI GGCC 1 cut(s) 263
BspEI TCCGGA 1 cut(s) 114
BspLI GGNNCC 1 cut(s) 280
BspPI GGATC 5 cut(s) 125, 144, 244, 273, 286
BspT104I TTCGAA 1 cut(s) 38
BspTNI GGTCTC 1 cut(s) 397
BsrI ACTGG 1 cut(s) 28
BssECI CCNNGG 2 cut(s) 232, 315
BssMI GATC 5 cut(s) 117, 136, 195, 236, 278
BssT1I CCWWGG 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 202
Bst6I CTCTTC 1 cut(s) 40
BstBI TTCGAA 1 cut(s) 38
BstDSI CCRYGG 1 cut(s) 232
BstKTI GATC 5 cut(s) 120, 139, 198, 239, 281
BstMAI GTCTC 5 cut(s) 88, 171, 191, 262, 397
BstMBI GATC 5 cut(s) 117, 136, 195, 236, 278
BstMWI GCNNNNNNNGC 2 cut(s) 304, 407
BstPAI GACNNNNGTC 1 cut(s) 183
BstSLI GKGCMC 1 cut(s) 16
BstV1I GCAGC 1 cut(s) 316
BstX2I RGATCY 3 cut(s) 117, 136, 278
BstYI RGATCY 3 cut(s) 117, 136, 278
BsuRI GGCC 1 cut(s) 263
BtgI CCRYGG 1 cut(s) 232
BtsIMutI CAGTG 2 cut(s) 372, 378
Cfr13I GGNCC 1 cut(s) 45
Csp6I GTAC 1 cut(s) 64
CviAII CATG 1 cut(s) 283
CviJI RGCY 6 cut(s) 78, 84, 263, 298, 356, 410
CviKI_1 RGCY 6 cut(s) 78, 84, 263, 298, 356, 410
CviQI GTAC 1 cut(s) 64
DpnI GATC 5 cut(s) 119, 138, 197, 238, 280
DpnII GATC 5 cut(s) 117, 136, 195, 236, 278
EaeI YGGCCR 1 cut(s) 261
Eam1104I CTCTTC 1 cut(s) 40
EarI CTCTTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 315
Eco24I GRGCYC 1 cut(s) 80
Eco31I GGTCTC 1 cut(s) 397
Eco47I GGWCC 1 cut(s) 45
Eco57I CTGAAG 1 cut(s) 350
EcoT14I CCWWGG 1 cut(s) 315
EcoT38I GRGCYC 1 cut(s) 80
ErhI CCWWGG 1 cut(s) 315
Esp3I CGTCTC 1 cut(s) 262
FaeI CATG 1 cut(s) 286
FaiI YATR 5 cut(s) 145, 284, 294, 309, 311
FatI CATG 1 cut(s) 282
FauI CCCGC 1 cut(s) 328
FauNDI CATATG 1 cut(s) 309
Fnu4HI GCNGC 2 cut(s) 207, 305
FriOI GRGCYC 1 cut(s) 80
Fsp4HI GCNGC 2 cut(s) 207, 305
FspBI CTAG 2 cut(s) 81, 353
GluI GCNGC 2 cut(s) 207, 305
HaeIII GGCC 1 cut(s) 263
HapII CCGG 2 cut(s) 115, 134
Hin1II CATG 1 cut(s) 286
HinfI GANTC 5 cut(s) 103, 127, 155, 179, 216
HpaII CCGG 2 cut(s) 115, 134
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 1 cut(s) 14
Hpy188I TCNGA 2 cut(s) 49, 93
Hpy188III TCNNGA 1 cut(s) 115
Hpy8I GTNNAC 1 cut(s) 14
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 2 cut(s) 181, 325
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4V TGCA 3 cut(s) 14, 286, 304
HpyF10VI GCNNNNNNNGC 2 cut(s) 304, 407
Hsp92II CATG 1 cut(s) 286
Kpn2I TCCGGA 1 cut(s) 114
Kzo9I GATC 5 cut(s) 117, 136, 195, 236, 278
LpnPI CCDG 4 cut(s) 41, 128, 147, 341
Lsp1109I GCAGC 1 cut(s) 316
MaeI CTAG 2 cut(s) 81, 353
MalI GATC 5 cut(s) 119, 138, 197, 238, 280
MboI GATC 5 cut(s) 117, 136, 195, 236, 278
MboII GAAGA 2 cut(s) 27, 281
MflI RGATCY 3 cut(s) 117, 136, 278
MhlI GDGCHC 2 cut(s) 16, 80
MluCI AATT 2 cut(s) 40, 50
MlyI GAGTC 2 cut(s) 149, 173
MnlI CCTC 4 cut(s) 43, 68, 313, 382
MroI TCCGGA 1 cut(s) 114
MspI CCGG 2 cut(s) 115, 134
Mva1269I GAATGC 2 cut(s) 329, 348
MwoI GCNNNNNNNGC 2 cut(s) 304, 407
NdeI CATATG 1 cut(s) 309
NdeII GATC 5 cut(s) 117, 136, 195, 236, 278
NlaIII CATG 1 cut(s) 286
NlaIV GGNNCC 1 cut(s) 280
NmeAIII GCCGAG 1 cut(s) 239
NspV TTCGAA 1 cut(s) 38
PctI GAATGC 2 cut(s) 329, 348
PfeI GAWTC 3 cut(s) 103, 127, 216
PflFI GACNNNGTC 1 cut(s) 254
PkrI GCNGC 2 cut(s) 208, 306
PleI GAGTC 2 cut(s) 149, 173
PpsI GAGTC 2 cut(s) 149, 173
PshAI GACNNNNGTC 1 cut(s) 183
PspN4I GGNNCC 1 cut(s) 280
PspPI GGNCC 1 cut(s) 45
PsuI RGATCY 3 cut(s) 117, 136, 278
PsyI GACNNNGTC 1 cut(s) 254
RsaI GTAC 1 cut(s) 65
RsaNI GTAC 1 cut(s) 64
SatI GCNGC 2 cut(s) 207, 305
Sau3AI GATC 5 cut(s) 117, 136, 195, 236, 278
Sau96I GGNCC 1 cut(s) 45
SchI GAGTC 2 cut(s) 149, 173
SduI GDGCHC 2 cut(s) 16, 80
SetI ASST 4 cut(s) 69, 86, 300, 412
SfuI TTCGAA 1 cut(s) 38
SinI GGWCC 1 cut(s) 45
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 2 cut(s) 40, 50
SsiI CCGC 3 cut(s) 7, 207, 335
SspMI CTAG 2 cut(s) 81, 353
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 1 cut(s) 202
TaqI TCGA 4 cut(s) 38, 153, 239, 267
TasI AATT 2 cut(s) 40, 50
TauI GCSGC 1 cut(s) 209
TfiI GAWTC 3 cut(s) 103, 127, 216
TscAI CASTG 2 cut(s) 379, 385
TseI GCWGC 1 cut(s) 304
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 249
TspRI CASTG 2 cut(s) 379, 385
Tth111I GACNNNGTC 1 cut(s) 254
VneI GTGCAC 1 cut(s) 12
VpaK11BI GGWCC 1 cut(s) 45
XspI CTAG 2 cut(s) 81, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.