MD17G1096900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006068
Physical Location & Seq
Forward (+)
8219867 .. 8220250
384 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1096900.v1.1.491

Sequence Viewer

Length: 384 bp
ATGAACCGCCGATCTAGATCCCTTCACCGCCGGTCCTCCCCCAACCGGTCGCCCGAGCCATTTCTCAAATACCTAAAACCCGGCGCGCTCGCTCAAATCCGAGACTCCCGAATCAGCAGGGCCAGATCGCACCAGCTCAATTGGCTCTCTCAGGTCCGTGTATCGCCGCCGTTGTCTCCAGTGTCCGACCCGGGTCCTCCCATGGTCAACGCGATGGAGGGATTTCCTTGCTTCTCGGGTCGGATCTTCGGCCCCAGATGCCCCCAGAGGAAGAAGCTCGCGGCCACAAAGTCCGTTATGTTCCTCAGCCCCTCGAGCCCGGTTCACGACTCGCCCGATCCGATAATCGATCTGTTTAGTACGGACATTCTTGCTGCACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

128

Amino Acids

14.16

Weight (kDa)

11.29

Isoelectric Point (pI)

83.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 86, 212, 281
AciI CCGC 4 cut(s) 7, 28, 167, 281
AclWI GGATC 3 cut(s) 12, 251, 332
AcoI YGGCCR 1 cut(s) 282
AfaI GTAC 1 cut(s) 361
AfiI CCNNNNNNNGG 1 cut(s) 45
AgeI ACCGGT 1 cut(s) 45
AluBI AGCT 2 cut(s) 136, 277
AluI AGCT 2 cut(s) 136, 277
Alw26I GTCTC 2 cut(s) 96, 180
AlwI GGATC 3 cut(s) 12, 251, 332
Ama87I CYCGRG 4 cut(s) 53, 190, 235, 313
AoxI GGCC 3 cut(s) 120, 250, 282
ApeKI GCWGC 1 cut(s) 374
AsiGI ACCGGT 1 cut(s) 45
AspLEI GCGC 2 cut(s) 86, 88
AspS9I GGNCC 5 cut(s) 33, 120, 154, 194, 251
AsuC2I CCSGG 4 cut(s) 81, 191, 192, 320
AsuHPI GGTGA 1 cut(s) 17
AvaI CYCGRG 4 cut(s) 53, 190, 235, 313
AvaII GGWCC 3 cut(s) 33, 154, 194
BanII GRGCYC 1 cut(s) 320
BbvCI CCTCAGC 1 cut(s) 305
BbvI GCAGC 1 cut(s) 361
BccI CCATC 1 cut(s) 208
BceAI ACGGC 1 cut(s) 154
BcnI CCSGG 4 cut(s) 81, 191, 192, 320
BcoDI GTCTC 2 cut(s) 96, 180
BfaI CTAG 1 cut(s) 15
BisI GCNGC 3 cut(s) 167, 282, 375
BlsI GCNGC 3 cut(s) 168, 283, 376
Bme1390I CCNGG 4 cut(s) 81, 191, 192, 320
Bme18I GGWCC 3 cut(s) 33, 154, 194
BmeT110I CYCGRG 4 cut(s) 53, 190, 235, 313
BmgT120I GGNCC 5 cut(s) 33, 120, 154, 194, 251
BmiI GGNNCC 2 cut(s) 195, 253
BmrFI CCNGG 4 cut(s) 81, 191, 192, 320
BmsI GCATC 1 cut(s) 248
BoxI GACNNNNGTC 1 cut(s) 192
BpmI CTGGAG 1 cut(s) 162
Bpu10I CCTNAGC 1 cut(s) 305
BpuMI CCSGG 4 cut(s) 81, 191, 192, 320
Bsa29I ATCGAT 1 cut(s) 348
BsaBI GATNNNNATC 1 cut(s) 16
BsaJI CCNNGG 2 cut(s) 190, 201
BsaWI WCCGGW 1 cut(s) 45
Bsc4I CCNNNNNNNGG 1 cut(s) 45
Bse118I RCCGGY 2 cut(s) 30, 45
Bse1I ACTGG 1 cut(s) 179
Bse8I GATNNNNATC 1 cut(s) 16
BseCI ATCGAT 1 cut(s) 348
BseDI CCNNGG 2 cut(s) 190, 201
BseJI GATNNNNATC 1 cut(s) 16
BseLI CCNNNNNNNGG 1 cut(s) 45
BseMII CTCAG 2 cut(s) 164, 319
BseNI ACTGG 1 cut(s) 179
BsePI GCGCGC 1 cut(s) 84
BseXI GCAGC 1 cut(s) 361
BsgI GTGCAG 1 cut(s) 360
Bsh1236I CGCG 3 cut(s) 86, 212, 281
Bsh1285I CGRYCG 1 cut(s) 50
BshFI GGCC 3 cut(s) 122, 252, 284
BshTI ACCGGT 1 cut(s) 45
BshVI ATCGAT 1 cut(s) 348
BsiEI CGRYCG 1 cut(s) 50
BsiHKCI CYCGRG 4 cut(s) 53, 190, 235, 313
BsiSI CCGG 5 cut(s) 31, 46, 81, 191, 320
BslI CCNNNNNNNGG 1 cut(s) 45
BsmAI GTCTC 2 cut(s) 96, 180
BsnI GGCC 3 cut(s) 122, 252, 284
BsoBI CYCGRG 4 cut(s) 53, 190, 235, 313
Bsp1286I GDGCHC 1 cut(s) 320
Bsp143I GATC 6 cut(s) 11, 17, 125, 243, 337, 349
Bsp19I CCATGG 1 cut(s) 201
BspACI CCGC 4 cut(s) 7, 28, 167, 281
BspANI GGCC 3 cut(s) 122, 252, 284
BspCNI CTCAG 2 cut(s) 163, 318
BspDI ATCGAT 1 cut(s) 348
BspFNI CGCG 3 cut(s) 86, 212, 281
BspLI GGNNCC 2 cut(s) 195, 253
BspPI GGATC 3 cut(s) 12, 251, 332
BsrFI RCCGGY 2 cut(s) 30, 45
BsrI ACTGG 1 cut(s) 179
BssAI RCCGGY 2 cut(s) 30, 45
BssECI CCNNGG 2 cut(s) 190, 201
BssHII GCGCGC 1 cut(s) 84
BssMI GATC 6 cut(s) 11, 17, 125, 243, 337, 349
BssT1I CCWWGG 1 cut(s) 201
BstC8I GCNNGC 3 cut(s) 86, 90, 279
BstDEI CTNAG 2 cut(s) 150, 305
BstDSI CCRYGG 1 cut(s) 201
BstFNI CGCG 3 cut(s) 86, 212, 281
BstHHI GCGC 2 cut(s) 86, 88
BstKTI GATC 6 cut(s) 14, 20, 128, 246, 340, 352
BstMAI GTCTC 2 cut(s) 96, 180
BstMBI GATC 6 cut(s) 11, 17, 125, 243, 337, 349
BstMCI CGRYCG 1 cut(s) 50
BstMWI GCNNNNNNNGC 3 cut(s) 142, 258, 315
BstPAI GACNNNNGTC 1 cut(s) 192
BstSCI CCNGG 4 cut(s) 79, 189, 190, 318
BstUI CGCG 3 cut(s) 86, 212, 281
BstV1I GCAGC 1 cut(s) 361
BstX2I RGATCY 2 cut(s) 17, 243
BstYI RGATCY 2 cut(s) 17, 243
Bsu15I ATCGAT 1 cut(s) 348
BsuRI GGCC 3 cut(s) 122, 252, 284
BsuTUI ATCGAT 1 cut(s) 348
BtgI CCRYGG 1 cut(s) 201
BtgZI GCGATG 1 cut(s) 227
BtsIMutI CAGTG 2 cut(s) 186, 379
Cac8I GCNNGC 3 cut(s) 86, 90, 279
CfoI GCGC 2 cut(s) 86, 88
Cfr10I RCCGGY 2 cut(s) 30, 45
Cfr13I GGNCC 5 cut(s) 33, 120, 154, 194, 251
Cfr9I CCCGGG 1 cut(s) 190
ClaI ATCGAT 1 cut(s) 348
Csp6I GTAC 1 cut(s) 360
CspAI ACCGGT 1 cut(s) 45
CviAII CATG 1 cut(s) 202
CviJI RGCY 9 cut(s) 58, 122, 136, 145, 252, 277, 284, 309, 318
CviKI_1 RGCY 9 cut(s) 58, 122, 136, 145, 252, 277, 284, 309, 318
CviQI GTAC 1 cut(s) 360
DdeI CTNAG 2 cut(s) 150, 305
DpnI GATC 6 cut(s) 13, 19, 127, 245, 339, 351
DpnII GATC 6 cut(s) 11, 17, 125, 243, 337, 349
EaeI YGGCCR 1 cut(s) 282
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 320
Eco47I GGWCC 3 cut(s) 33, 154, 194
Eco88I CYCGRG 4 cut(s) 53, 190, 235, 313
EcoO109I RGGNCCY 1 cut(s) 194
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 320
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 1 cut(s) 205
FaiI YATR 2 cut(s) 203, 299
FatI CATG 1 cut(s) 201
Fnu4HI GCNGC 3 cut(s) 167, 282, 375
FriOI GRGCYC 1 cut(s) 320
Fsp4HI GCNGC 3 cut(s) 167, 282, 375
FspBI CTAG 1 cut(s) 15
GlaI GCGC 2 cut(s) 85, 87
GluI GCNGC 3 cut(s) 167, 282, 375
GsuI CTGGAG 1 cut(s) 162
HaeIII GGCC 3 cut(s) 122, 252, 284
HapII CCGG 5 cut(s) 31, 46, 81, 191, 320
HhaI GCGC 2 cut(s) 86, 88
Hin1II CATG 1 cut(s) 205
Hin6I GCGC 2 cut(s) 84, 86
HinP1I GCGC 2 cut(s) 84, 86
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HinfI GANTC 3 cut(s) 104, 111, 329
HpaII CCGG 5 cut(s) 31, 46, 81, 191, 320
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 2 cut(s) 208, 325
Hpy188I TCNGA 4 cut(s) 101, 187, 243, 342
Hpy188III TCNNGA 3 cut(s) 15, 108, 326
Hpy8I GTNNAC 2 cut(s) 208, 325
HpyAV CCTTC 1 cut(s) 32
HpyCH4V TGCA 1 cut(s) 377
HpyF10VI GCNNNNNNNGC 3 cut(s) 142, 258, 315
HpyF3I CTNAG 2 cut(s) 150, 305
Hsp92II CATG 1 cut(s) 205
HspAI GCGC 2 cut(s) 84, 86
Kzo9I GATC 6 cut(s) 11, 17, 125, 243, 337, 349
Lsp1109I GCAGC 1 cut(s) 361
LweI GCATC 1 cut(s) 248
MaeI CTAG 1 cut(s) 15
MalI GATC 6 cut(s) 13, 19, 127, 245, 339, 351
MboI GATC 6 cut(s) 11, 17, 125, 243, 337, 349
MboII GAAGA 2 cut(s) 238, 283
MfeI CAATTG 1 cut(s) 139
MflI RGATCY 2 cut(s) 17, 243
MhlI GDGCHC 1 cut(s) 320
MluCI AATT 1 cut(s) 139
MlyI GAGTC 2 cut(s) 98, 323
MmeI TCCRAC 2 cut(s) 210, 221
MnlI CCTC 6 cut(s) 46, 207, 211, 261, 314, 322
MspI CCGG 5 cut(s) 31, 46, 81, 191, 320
MspR9I CCNGG 4 cut(s) 81, 191, 192, 320
MunI CAATTG 1 cut(s) 139
MvnI CGCG 3 cut(s) 86, 212, 281
MwoI GCNNNNNNNGC 3 cut(s) 142, 258, 315
NciI CCSGG 4 cut(s) 81, 191, 192, 320
NcoI CCATGG 1 cut(s) 201
NdeII GATC 6 cut(s) 11, 17, 125, 243, 337, 349
NlaIII CATG 1 cut(s) 205
NlaIV GGNNCC 2 cut(s) 195, 253
PaeR7I CTCGAG 1 cut(s) 313
PauI GCGCGC 1 cut(s) 84
PcsI WCGNNNNNNNCGW 2 cut(s) 333, 338
PfeI GAWTC 1 cut(s) 111
PinAI ACCGGT 1 cut(s) 45
PkrI GCNGC 3 cut(s) 168, 283, 376
PleI GAGTC 2 cut(s) 98, 323
PpsI GAGTC 2 cut(s) 98, 323
PpuMI RGGWCCY 1 cut(s) 194
PshAI GACNNNNGTC 1 cut(s) 192
Psp5II RGGWCCY 1 cut(s) 194
PspN4I GGNNCC 2 cut(s) 195, 253
PspPI GGNCC 5 cut(s) 33, 120, 154, 194, 251
PspPPI RGGWCCY 1 cut(s) 194
PspXI VCTCGAGB 1 cut(s) 313
PsuI RGATCY 2 cut(s) 17, 243
PteI GCGCGC 1 cut(s) 84
RsaI GTAC 1 cut(s) 361
RsaNI GTAC 1 cut(s) 360
SatI GCNGC 3 cut(s) 167, 282, 375
Sau3AI GATC 6 cut(s) 11, 17, 125, 243, 337, 349
Sau96I GGNCC 5 cut(s) 33, 120, 154, 194, 251
SchI GAGTC 2 cut(s) 98, 323
ScrFI CCNGG 4 cut(s) 81, 191, 192, 320
SduI GDGCHC 1 cut(s) 320
SetI ASST 4 cut(s) 75, 138, 156, 279
SfaNI GCATC 1 cut(s) 248
Sfr274I CTCGAG 1 cut(s) 313
SinI GGWCC 3 cut(s) 33, 154, 194
SlaI CTCGAG 1 cut(s) 313
SmaI CCCGGG 1 cut(s) 192
SmlI CTYRAG 1 cut(s) 313
SmoI CTYRAG 1 cut(s) 313
Sse9I AATT 1 cut(s) 139
SsiI CCGC 4 cut(s) 7, 28, 167, 281
SspMI CTAG 1 cut(s) 15
StyD4I CCNGG 4 cut(s) 79, 189, 190, 318
StyI CCWWGG 1 cut(s) 201
TaqI TCGA 2 cut(s) 314, 348
TasI AATT 1 cut(s) 139
TauI GCSGC 2 cut(s) 169, 284
TfiI GAWTC 1 cut(s) 111
TscAI CASTG 1 cut(s) 186
TseI GCWGC 1 cut(s) 374
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 3 cut(s) 146, 283, 377
TspMI CCCGGG 1 cut(s) 190
TspRI CASTG 1 cut(s) 186
VpaK11BI GGWCC 3 cut(s) 33, 154, 194
XbaI TCTAGA 1 cut(s) 14
XhoI CTCGAG 1 cut(s) 313
XmaI CCCGGG 1 cut(s) 190
XspI CTAG 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.