MD09G1109200.v1.1

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005671
Physical Location & Seq
Reverse (-)
8076667 .. 8082387
5721 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1109200.v1.1.491

Sequence Viewer

Length: 2844 bp
ATGGAGGCGAAAGGAAGAAACCTCAAGATTGCAGTAGTCGCCCTCGCTTCTCTGACAGCTCTCTCCGTCTTCTTCTTTGCGCGGCACCGGAGACGCCGCCGGAACGCAAACTGCCCCCTAAGTCGATGTTATTTACGAGCCGATCACCTCAAGCTGCAGTACGGATTCAAGCGAGTTTTAGCTGACAATTCCTACTCTCCCTTCAAGCATCTGAACTTGAATGGTTCTCCGGAAGATAAGAACTGGAATTCGCATCCTTATGAGGCTGAAATCACGGGATTGGTGGAAAGTTTTCAGCTTGAGGAGTTTCGAAAATGTATTGGAATTACAGAGAAGAATGTGGGGGGTTTGGAAGAAATGAGTGAGTCGTATGAGTGGGTGGAGACCGAGTCGAAGTTGAAGGAACTCGTTGAGGTTTTGAACAAAGAGAAGGTATTTGCTGTTGATACGGAGCAGCATAGTTTTCGGTCTTTTCTCGGTTTTACAGCTCTAATACAGATTTCTACTGAGAAGGAAGATTACTTGGTGGACACGATTGCTCTGCATGATTGCATAAGCCTTCTACGGCCGGTTTTCGCTGATGCTAGTATTTGTAAGGTGTTCCATGGGGCGGATAGTGATGTTCTCTGGCTGCAAAGGGACTTCCATATTTATGTTGTTAATATGTTTGATACTGCAAAGGCTTGTGAAGTTTTGTCAAAGCCGCAGAAGTCACTGGCATATTTGCTTGAATCTTACTGTGGAGTAGTCACTAACAAGTTGTTACAGCGTGAAGACTGGAGACAGCGCCCCTTGTCAATTGAAATGGTACAATATGCTCGTACAGATGCGCACTATCTACTCTATATTGCACGCAGTTTAATTGCTGAGCTGGAAATGCTGGATAACGAAAACAACGGTCCCAATGACAAATTACATTTTGTTCTCGAGGCTAGTCGGCGTTCAAACTCGATATGTTTGCAACTGTACACTAAAGAGATAGAAGCTTCTCCTGGAGAGTCTGCTGCTTCATCAATTTTTAGCCGTCATTTGAACGGCCAAGGAGGGATTTCATCAGTTTCTCCTGAAGTCCAGGATGCTGTGAACAGATTATGTGCATGGAGAGATTTGATGGCTCGTGTACACGATGAGAGCTTAAGATATGTAATATCAGATCAGGCTATTGTTGCCCTTGCAGACAAAGCTCCAACGAACCCTGCAGACATATTAACCACCATTGCTGAAGCTGATTGCAATGCAGATTCAAGTTTTGGTTCTGGCCTCTCATCTCCATCCCCTGTCGTTTACAGTCATTTGGATGACTTTCGTCATCTGCTCCATAACAAAATAGGCAACCCTGATAATTTATTTTCTAAGATTCTTCAAAACTGCCTGGGTCCAAATGGGAGTTGCCCACTGTCCATCTTCAATTATGCTTTACTGGTAGAGTGTAATATGAAAGTGCCACTAACTTCTAGAAAAAATGGGTCGAGAAATTCAAAGCAAGTTGCTCGAAAGTCGTCTCGGCAGCTATTTGTTCAAAAATTTTCTTGTAAAGCTCCAGTTTATCATAATTGCAGGATCTATGCAGATGATGGCCGGTTGCTTTGTTACTGTGACCGCAGGAAGCTTGAATGGTATCTTCGGCAGGATTTGGCAAAACTTGTTGATGAAAATCCACCTGCAATTATGCTTCTTTTTGAGCCCAAAGGCCGTCCAGAAGATGAAGGCAATGATTTTTACATTCAAAGTAAGAAAAATATATGTGTTAGCTGTGGCGAAAGAAATCATTACTTACGCTACCGAATAATTCCCTCATGCTACAGAATGCACTTCCCTGAACATTTGAAAAGCCACCGTTCTCATGATATTGTCCTACTATGTGTGGACTGTCATGAAATTGCTCATGCCTCTGCTGAGAAGTATAAAAAACATATCGCTGCAGAATTTGGGATTCCACTTTTTGTTCGCAGAGTGGTTGACTCAGAACAATCTCAGGCTACATCTGAAGCATCTGTTCCAGAAACAACTTCTGAGGAACCAGGTGTATCTCCTTTACAATTGCGCATTGCTGCTATGGCTCTTCTGCGCCATGGACCAAGAATGCCATCCAGCCGCCGTGAAGAACTGACAGAGATAGTAATGAGGTATTATGGAGGGAGGCAAATATCTGAGGAAGATTTAGAAAACGCTTTGTTGGTTGGCATGAGTCCCAACGAGAGAAGACAATTTGAGAAGAAGAAAGGACTTTCTTTAAAGCATTCTAAGGGGATTATTCAACCAGATGCAGAGCAGGAGAGAACTACAATTACCATGTACACATCTGCCATTGTGAACGCATCGGAAGTTGATCCTCTACTTGATTCATGCACCACTGAAGCAGAAGCATATACCGGAACAGAAAATGGAGATGCTTTCATGCTGAAGGATGCTGATTTGGGGACTGAAGAAGAAGCTTTTGCTGTTGTTGAAAAGAACATGAACTCAGATATAAGTGGAGTTGCTGATAGTCAGTTTATATCTGTTGTAAATGCTGATGGTGACGGTGATGGTGAGAGAGATACACCGAACGGATCAGTTGATTTTAGTCATGCCGGATATGGTGCAACTTCCCACCGTAAACGTAGATCAAAAATGTCACTTTTAGGACATGGTCCACATGGCAAGCAAGTTGTGGATCATCTGCTAAAGGAATATGGTGAAGATGGCGTTTCTCAATTTTGTCAAAGGTGGAGACAAGTATTCGTTGAATCTCTTCATCCTCGTTTTTTACCTGCCGGCTGGGATATAACGCACAGGAGGGACTTCGGCGAATTTAGCGTCTACAATCCTCTCAAGAAGGCTTCTGCTGCTGAATGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0000178 GO:0000460 GO:0000785 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006403 GO:0006725 GO:0006807 GO:0007549 GO:0008150 GO:0008152 GO:0008298 GO:0008334 GO:0008408 GO:0009048 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010639 GO:0016070 GO:0016071 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032210 GO:0032211 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035327 GO:0040029 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045934 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051179 GO:0051641 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071025 GO:0071027 GO:0071028 GO:0071029 GO:0071030 GO:0071033 GO:0071034 GO:0071035 GO:0071043 GO:0071044 GO:0071046 GO:0071048 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902494 GO:1904356 GO:1904357 GO:1904872 GO:1905354 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1 HRDC

Protein Analysis

948

Amino Acids

106.87

Weight (kDa)

6.15

Isoelectric Point (pI)

52.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 124 - 291 2.3e-50 3'-5' exonuclease
HRDC PF00570 359 - 403 5e-08 HRDC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1669
Acc16I TGCGCA 2 cut(s) 831, 2045
Acc36I ACCTGC 2 cut(s) 1669, 2761
AccB1I GGYRCC 1 cut(s) 84
AccI GTMKAC 1 cut(s) 2802
AccII CGCG 1 cut(s) 82
AccIII TCCGGA 1 cut(s) 229
AciI CCGC 6 cut(s) 82, 97, 611, 704, 1600, 2095
AclWI GGATC 4 cut(s) 1568, 2324, 2560, 2664
AcoI YGGCCR 3 cut(s) 566, 1036, 1576
AcsI RAATTY 5 cut(s) 247, 1474, 1523, 1925, 2792
AcuI CTGAAG 6 cut(s) 1086, 1242, 2007, 2376, 2423, 2445
AcyI GRCGYC 1 cut(s) 94
AfaI GTAC 6 cut(s) 161, 810, 823, 968, 1122, 2297
AfiI CCNNNNNNNGG 1 cut(s) 610
AflII CTTAAG 1 cut(s) 1135
AjnI CCWGG 4 cut(s) 991, 1071, 1371, 2020
Alw26I GTCTC 5 cut(s) 85, 377, 775, 1506, 2707
AlwI GGATC 4 cut(s) 1568, 2324, 2560, 2664
Ama87I CYCGRG 1 cut(s) 926
Aor13HI TCCGGA 1 cut(s) 229
AoxI GGCC 5 cut(s) 566, 1036, 1258, 1576, 1690
ApeKI GCWGC 8 cut(s) 154, 454, 631, 1004, 1507, 1919, 2051, 2828
ApoI RAATTY 5 cut(s) 247, 1474, 1523, 1925, 2792
ArsI GACNNNNNNTTYG 2 cut(s) 2784, 2816
Asp700I GAANNNNTTC 2 cut(s) 291, 2733
AspLEI GCGC 5 cut(s) 82, 789, 832, 2046, 2070
AspS9I GGNCC 4 cut(s) 899, 1376, 2075, 2633
AsuHPI GGTGA 5 cut(s) 137, 2531, 2537, 2543, 2690
AsuII TTCGAA 1 cut(s) 310
AvaI CYCGRG 1 cut(s) 926
AvaII GGWCC 4 cut(s) 899, 1376, 2075, 2633
BanI GGYRCC 1 cut(s) 84
BanII GRGCYC 1 cut(s) 1686
BarI GAAGNNNNNNTAC 2 cut(s) 503, 535
BauI CACGAG 1 cut(s) 1116
BbsI GAAGAC 3 cut(s) 61, 780, 2209
BbvI GCAGC 8 cut(s) 141, 466, 618, 991, 1519, 1906, 2038, 2815
BccI CCATC 8 cut(s) 1105, 1279, 1409, 1568, 2095, 2510, 2522, 2678
BceAI ACGGC 5 cut(s) 581, 1008, 1051, 1677, 2082
BcgI CGANNNNNNTGC 8 cut(s) 446, 480, 523, 557, 940, 974, 1472, 1506
BciT130I CCWGG 4 cut(s) 993, 1073, 1373, 2022
BcoDI GTCTC 5 cut(s) 85, 377, 775, 1506, 2707
BfaI CTAG 3 cut(s) 585, 933, 1455
BfmI CTRYAG 4 cut(s) 155, 1197, 1801, 1920
BfoI RGCGCY 1 cut(s) 790
BfrI CTTAAG 1 cut(s) 1135
BfuAI ACCTGC 2 cut(s) 1669, 2761
BlpI GCTNAGC 1 cut(s) 867
Bme1390I CCNGG 4 cut(s) 993, 1073, 1373, 2022
Bme18I GGWCC 4 cut(s) 899, 1376, 2075, 2633
BmeT110I CYCGRG 1 cut(s) 926
BmgT120I GGNCC 4 cut(s) 899, 1376, 2075, 2633
BmiI GGNNCC 4 cut(s) 86, 901, 1377, 2019
BmrFI CCNGG 4 cut(s) 993, 1073, 1373, 2022
BoxI GACNNNNGTC 1 cut(s) 1305
BpiI GAAGAC 3 cut(s) 61, 780, 2209
BpmI CTGGAG 3 cut(s) 799, 1014, 1524
Bpu1102I GCTNAGC 1 cut(s) 867
Bpu14I TTCGAA 1 cut(s) 310
BpuEI CTTGAG 4 cut(s) 8, 134, 320, 2798
BsaBI GATNNNNATC 1 cut(s) 1653
BsaHI GRCGYC 1 cut(s) 94
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 4 cut(s) 604, 1039, 1372, 2071
BsaWI WCCGGW 3 cut(s) 87, 229, 2372
BsaXI ACNNNNNCTCC 2 cut(s) 374, 404
Bsc4I CCNNNNNNNGG 1 cut(s) 610
Bse118I RCCGGY 3 cut(s) 568, 1578, 2756
Bse1I ACTGG 5 cut(s) 248, 720, 782, 1425, 1541
Bse3DI GCAATG 4 cut(s) 1215, 1240, 1717, 2046
Bse8I GATNNNNATC 1 cut(s) 1653
BseAI TCCGGA 1 cut(s) 229
BseBI CCWGG 4 cut(s) 993, 1073, 1373, 2022
BseDI CCNNGG 4 cut(s) 604, 1039, 1372, 2071
BseGI GGATG 7 cut(s) 253, 1081, 1271, 1303, 2087, 2413, 2737
BseJI GATNNNNATC 1 cut(s) 1653
BseLI CCNNNNNNNGG 1 cut(s) 610
BseMI GCAATG 4 cut(s) 1215, 1240, 1717, 2046
BseMII CTCAG 8 cut(s) 498, 858, 1887, 1977, 1988, 2004, 2142, 2478
BseNI ACTGG 5 cut(s) 248, 720, 782, 1425, 1541
BseRI GAGGAG 1 cut(s) 317
BseX3I CGGCCG 1 cut(s) 566
BseXI GCAGC 8 cut(s) 141, 466, 618, 991, 1519, 1906, 2038, 2815
BseYI CCCAGC 1 cut(s) 2760
Bsh1236I CGCG 1 cut(s) 82
Bsh1285I CGRYCG 1 cut(s) 569
BshFI GGCC 5 cut(s) 568, 1038, 1260, 1578, 1692
BshNI GGYRCC 1 cut(s) 84
BsiEI CGRYCG 1 cut(s) 569
BsiHKCI CYCGRG 1 cut(s) 926
BsiSI CCGG 8 cut(s) 88, 100, 230, 569, 1579, 2373, 2574, 2757
BslFI GGGAC 5 cut(s) 653, 885, 2175, 2434, 2795
BslI CCNNNNNNNGG 1 cut(s) 610
BsmAI GTCTC 5 cut(s) 85, 377, 775, 1506, 2707
BsmBI CGTCTC 2 cut(s) 85, 1506
BsmFI GGGAC 5 cut(s) 653, 885, 2175, 2434, 2795
BsmI GAATGC 3 cut(s) 1812, 2088, 2239
BsnI GGCC 5 cut(s) 568, 1038, 1260, 1578, 1692
Bso31I GGTCTC 1 cut(s) 377
BsoBI CYCGRG 1 cut(s) 926
Bsp119I TTCGAA 1 cut(s) 310
Bsp1286I GDGCHC 1 cut(s) 1686
Bsp13I TCCGGA 1 cut(s) 229
Bsp1407I TGTACA 3 cut(s) 966, 1120, 2295
Bsp143I GATC 7 cut(s) 142, 1153, 1560, 2329, 2552, 2606, 2656
Bsp1720I GCTNAGC 1 cut(s) 867
Bsp19I CCATGG 2 cut(s) 604, 2071
BspACI CCGC 6 cut(s) 82, 97, 611, 704, 1600, 2095
BspANI GGCC 5 cut(s) 568, 1038, 1260, 1578, 1692
BspCNI CTCAG 8 cut(s) 499, 859, 1888, 1976, 1987, 2005, 2143, 2477
BspEI TCCGGA 1 cut(s) 229
BspFNI CGCG 1 cut(s) 82
BspHI TCATGA 2 cut(s) 1843, 1873
BspLI GGNNCC 4 cut(s) 86, 901, 1377, 2019
BspMAI CTGCAG 3 cut(s) 159, 1201, 1924
BspMI ACCTGC 2 cut(s) 1669, 2761
BspPI GGATC 4 cut(s) 1568, 2324, 2560, 2664
BspQI GCTCTTC 1 cut(s) 2067
BspT104I TTCGAA 1 cut(s) 310
BspT107I GGYRCC 1 cut(s) 84
BspTI CTTAAG 1 cut(s) 1135
BspTNI GGTCTC 1 cut(s) 377
BsrDI GCAATG 4 cut(s) 1215, 1240, 1717, 2046
BsrFI RCCGGY 3 cut(s) 568, 1578, 2756
BsrGI TGTACA 3 cut(s) 966, 1120, 2295
BsrI ACTGG 5 cut(s) 248, 720, 782, 1425, 1541
BssAI RCCGGY 3 cut(s) 568, 1578, 2756
BssECI CCNNGG 4 cut(s) 604, 1039, 1372, 2071
BssMI GATC 7 cut(s) 142, 1153, 1560, 2329, 2552, 2606, 2656
BssNI GRCGYC 1 cut(s) 94
BssSI CACGAG 1 cut(s) 1116
BssT1I CCWWGG 3 cut(s) 604, 1039, 2071
Bst2BI CACGAG 1 cut(s) 1116
Bst2UI CCWGG 4 cut(s) 993, 1073, 1373, 2022
Bst6I CTCTTC 2 cut(s) 2067, 2739
BstACI GRCGYC 1 cut(s) 94
BstAFI CTTAAG 1 cut(s) 1135
BstAUI TGTACA 3 cut(s) 966, 1120, 2295
BstBI TTCGAA 1 cut(s) 310
BstC8I GCNNGC 3 cut(s) 853, 2645, 2758
BstDSI CCRYGG 2 cut(s) 604, 2071
BstF5I GGATG 7 cut(s) 253, 1081, 1271, 1303, 2087, 2413, 2737
BstFNI CGCG 1 cut(s) 82
BstH2I RGCGCY 1 cut(s) 790
BstHHI GCGC 5 cut(s) 82, 789, 832, 2046, 2070
BstKTI GATC 7 cut(s) 145, 1156, 1563, 2332, 2555, 2609, 2659
BstMAI GTCTC 5 cut(s) 85, 377, 775, 1506, 2707
BstMBI GATC 7 cut(s) 142, 1153, 1560, 2329, 2552, 2606, 2656
BstMCI CGRYCG 1 cut(s) 569
BstMWI GCNNNNNNNGC 7 cut(s) 38, 877, 1166, 1181, 2057, 2796, 2828
BstNI CCWGG 4 cut(s) 993, 1073, 1373, 2022
BstPAI GACNNNNGTC 1 cut(s) 1305
BstSCI CCNGG 4 cut(s) 991, 1071, 1371, 2020
BstSFI CTRYAG 4 cut(s) 155, 1197, 1801, 1920
BstUI CGCG 1 cut(s) 82
BstV1I GCAGC 8 cut(s) 141, 466, 618, 991, 1519, 1906, 2038, 2815
BstV2I GAAGAC 3 cut(s) 61, 780, 2209
BstX2I RGATCY 1 cut(s) 1560
BstYI RGATCY 1 cut(s) 1560
BstZI CGGCCG 1 cut(s) 566
BsuRI GGCC 5 cut(s) 568, 1038, 1260, 1578, 1692
BtgI CCRYGG 2 cut(s) 604, 2071
BtsCI GGATG 7 cut(s) 253, 1081, 1271, 1303, 2087, 2413, 2737
BtsIMutI CAGTG 3 cut(s) 713, 1394, 2352
BveI ACCTGC 2 cut(s) 1669, 2761
Cac8I GCNNGC 3 cut(s) 853, 2645, 2758
CciI TCATGA 2 cut(s) 1843, 1873
CfoI GCGC 5 cut(s) 82, 789, 832, 2046, 2070
Cfr10I RCCGGY 3 cut(s) 568, 1578, 2756
Cfr13I GGNCC 4 cut(s) 899, 1376, 2075, 2633
CseI GACGC 2 cut(s) 102, 2788
CsiI ACCWGGT 1 cut(s) 2020
Csp6I GTAC 6 cut(s) 160, 809, 822, 967, 1121, 2296
CviQI GTAC 6 cut(s) 160, 809, 822, 967, 1121, 2296
DpnI GATC 7 cut(s) 144, 1155, 1562, 2331, 2554, 2608, 2658
DpnII GATC 7 cut(s) 142, 1153, 1560, 2329, 2552, 2606, 2656
DraI TTTAAA 1 cut(s) 2235
EaeI YGGCCR 3 cut(s) 566, 1036, 1576
EagI CGGCCG 1 cut(s) 566
Eam1104I CTCTTC 2 cut(s) 2067, 2739
EarI CTCTTC 2 cut(s) 2067, 2739
EciI GGCGGA 1 cut(s) 626
EclXI CGGCCG 1 cut(s) 566
Eco130I CCWWGG 3 cut(s) 604, 1039, 2071
Eco24I GRGCYC 1 cut(s) 1686
Eco31I GGTCTC 1 cut(s) 377
Eco47I GGWCC 4 cut(s) 899, 1376, 2075, 2633
Eco52I CGGCCG 1 cut(s) 566
Eco57I CTGAAG 6 cut(s) 1086, 1242, 2007, 2376, 2423, 2445
Eco88I CYCGRG 1 cut(s) 926
EcoRI GAATTC 1 cut(s) 247
EcoRII CCWGG 4 cut(s) 991, 1071, 1371, 2020
EcoT14I CCWWGG 3 cut(s) 604, 1039, 2071
EcoT38I GRGCYC 1 cut(s) 1686
ErhI CCWWGG 3 cut(s) 604, 1039, 2071
Esp3I CGTCTC 2 cut(s) 85, 1506
FaqI GGGAC 5 cut(s) 653, 885, 2175, 2434, 2795
FblI GTMKAC 1 cut(s) 2802
FokI GGATG 7 cut(s) 240, 1088, 1258, 1310, 2074, 2420, 2724
FriOI GRGCYC 1 cut(s) 1686
FspAI RTGCGCAY 1 cut(s) 831
FspBI CTAG 3 cut(s) 585, 933, 1455
FspI TGCGCA 2 cut(s) 831, 2045
GlaI GCGC 5 cut(s) 81, 788, 831, 2045, 2069
GsaI CCCAGC 1 cut(s) 2764
GsuI CTGGAG 3 cut(s) 799, 1014, 1524
HaeII RGCGCY 1 cut(s) 790
HaeIII GGCC 5 cut(s) 568, 1038, 1260, 1578, 1692
HapII CCGG 8 cut(s) 88, 100, 230, 569, 1579, 2373, 2574, 2757
HgaI GACGC 2 cut(s) 102, 2788
HhaI GCGC 5 cut(s) 82, 789, 832, 2046, 2070
Hin1I GRCGYC 1 cut(s) 94
Hin6I GCGC 5 cut(s) 80, 787, 830, 2044, 2068
HinP1I GCGC 5 cut(s) 80, 787, 830, 2044, 2068
HincII GTYRAC 1 cut(s) 1960
HindII GTYRAC 1 cut(s) 1960
HindIII AAGCTT 3 cut(s) 984, 1607, 2433
HpaII CCGG 8 cut(s) 88, 100, 230, 569, 1579, 2373, 2574, 2757
HphI GGTGA 5 cut(s) 137, 2531, 2537, 2543, 2690
Hpy188I TCNGA 9 cut(s) 54, 213, 1153, 1966, 1987, 2014, 2152, 2323, 2467
HpyAV CCTTC 8 cut(s) 211, 394, 424, 505, 569, 1700, 2398, 2812
HpyCH4IV ACGT 1 cut(s) 2602
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 877, 1166, 1181, 2057, 2796, 2828
HpySE526I ACGT 1 cut(s) 2602
Hsp92I GRCGYC 1 cut(s) 94
HspAI GCGC 5 cut(s) 80, 787, 830, 2044, 2068
Kpn2I TCCGGA 1 cut(s) 229
KroI GCCGGC 1 cut(s) 2756
KroNI GCCGGC 1 cut(s) 2758
Kzo9I GATC 7 cut(s) 142, 1153, 1560, 2329, 2552, 2606, 2656
LguI GCTCTTC 1 cut(s) 2067
LmnI GCTCC 4 cut(s) 451, 1189, 1320, 1543
Lsp1109I GCAGC 8 cut(s) 141, 466, 618, 991, 1519, 1906, 2038, 2815
MabI ACCWGGT 1 cut(s) 2020
MaeI CTAG 3 cut(s) 585, 933, 1455
MaeII ACGT 1 cut(s) 2602
MaeIII GTNAC 7 cut(s) 711, 748, 762, 1589, 1595, 2519, 2616
MalI GATC 7 cut(s) 144, 1155, 1562, 2331, 2554, 2608, 2658
MboI GATC 7 cut(s) 142, 1153, 1560, 2329, 2552, 2606, 2656
MfeI CAATTG 2 cut(s) 798, 2039
MflI RGATCY 1 cut(s) 1560
MhlI GDGCHC 1 cut(s) 1686
MlyI GAGTC 5 cut(s) 374, 398, 1007, 1955, 2197
MmeI TCCRAC 1 cut(s) 1211
MroI TCCGGA 1 cut(s) 229
MroNI GCCGGC 1 cut(s) 2756
MroXI GAANNNNTTC 2 cut(s) 291, 2733
MseI TTAA 5 cut(s) 660, 860, 1136, 1208, 2234
MslI CAYNNNNRTG 3 cut(s) 258, 651, 1296
MspCI CTTAAG 1 cut(s) 1135
MspI CCGG 8 cut(s) 88, 100, 230, 569, 1579, 2373, 2574, 2757
MspR9I CCNGG 4 cut(s) 993, 1073, 1373, 2022
MunI CAATTG 2 cut(s) 798, 2039
Mva1269I GAATGC 3 cut(s) 1812, 2088, 2239
MvaI CCWGG 4 cut(s) 993, 1073, 1373, 2022
MvnI CGCG 1 cut(s) 82
MwoI GCNNNNNNNGC 7 cut(s) 38, 877, 1166, 1181, 2057, 2796, 2828
NaeI GCCGGC 1 cut(s) 2758
NcoI CCATGG 2 cut(s) 604, 2071
NdeII GATC 7 cut(s) 142, 1153, 1560, 2329, 2552, 2606, 2656
NgoMIV GCCGGC 1 cut(s) 2756
NlaIV GGNNCC 4 cut(s) 86, 901, 1377, 2019
NmeAIII GCCGAG 1 cut(s) 1483
NmuCI GTSAC 5 cut(s) 711, 748, 1595, 2519, 2616
NsbI TGCGCA 2 cut(s) 831, 2045
NspV TTCGAA 1 cut(s) 310
PaeR7I CTCGAG 1 cut(s) 926
PagI TCATGA 2 cut(s) 1843, 1873
PaqCI CACCTGC 1 cut(s) 1669
PciSI GCTCTTC 1 cut(s) 2067
PctI GAATGC 3 cut(s) 1812, 2088, 2239
PdiI GCCGGC 1 cut(s) 2758
PdmI GAANNNNTTC 2 cut(s) 291, 2733
PfeI GAWTC 7 cut(s) 165, 731, 1241, 1357, 1933, 2342, 2729
PflFI GACNNNGTC 2 cut(s) 388, 2631
PfoI TCCNGGA 2 cut(s) 991, 1071
PleI GAGTC 5 cut(s) 373, 397, 1006, 1955, 2196
PpsI GAGTC 5 cut(s) 373, 397, 1006, 1955, 2196
PshAI GACNNNNGTC 1 cut(s) 1305
Psp6I CCWGG 4 cut(s) 991, 1071, 1371, 2020
PspFI CCCAGC 1 cut(s) 2760
PspGI CCWGG 4 cut(s) 991, 1071, 1371, 2020
PspN4I GGNNCC 4 cut(s) 86, 901, 1377, 2019
PspPI GGNCC 4 cut(s) 899, 1376, 2075, 2633
PsrI GAACNNNNNNTAC 2 cut(s) 2272, 2304
PstI CTGCAG 3 cut(s) 159, 1201, 1924
PsuI RGATCY 1 cut(s) 1560
PsyI GACNNNGTC 2 cut(s) 388, 2631
RsaI GTAC 6 cut(s) 161, 810, 823, 968, 1122, 2297
RsaNI GTAC 6 cut(s) 160, 809, 822, 967, 1121, 2296
RseI CAYNNNNRTG 3 cut(s) 258, 651, 1296
SapI GCTCTTC 1 cut(s) 2067
SaqAI TTAA 5 cut(s) 660, 860, 1136, 1208, 2234
Sau3AI GATC 7 cut(s) 142, 1153, 1560, 2329, 2552, 2606, 2656
Sau96I GGNCC 4 cut(s) 899, 1376, 2075, 2633
SchI GAGTC 5 cut(s) 374, 398, 1007, 1955, 2197
ScrFI CCNGG 4 cut(s) 993, 1073, 1373, 2022
SduI GDGCHC 1 cut(s) 1686
SexAI ACCWGGT 1 cut(s) 2020
SfcI CTRYAG 4 cut(s) 155, 1197, 1801, 1920
Sfr274I CTCGAG 1 cut(s) 926
SfuI TTCGAA 1 cut(s) 310
SinI GGWCC 4 cut(s) 899, 1376, 2075, 2633
SlaI CTCGAG 1 cut(s) 926
SmiMI CAYNNNNRTG 3 cut(s) 258, 651, 1296
SmlI CTYRAG 6 cut(s) 23, 149, 299, 926, 1135, 2813
SmoI CTYRAG 6 cut(s) 23, 149, 299, 926, 1135, 2813
SsiI CCGC 6 cut(s) 82, 97, 611, 704, 1600, 2095
SspMI CTAG 3 cut(s) 585, 933, 1455
StyD4I CCNGG 4 cut(s) 991, 1071, 1371, 2020
StyI CCWWGG 3 cut(s) 604, 1039, 2071
TaiI ACGT 1 cut(s) 2605
TaqI TCGA 7 cut(s) 124, 310, 392, 927, 950, 1469, 1492
TaqII GACCGA 2 cut(s) 401, 456
TatI WGTACW 3 cut(s) 966, 1120, 2295
TauI GCSGC 4 cut(s) 85, 99, 706, 2097
TfiI GAWTC 7 cut(s) 165, 731, 1241, 1357, 1933, 2342, 2729
Tru1I TTAA 5 cut(s) 660, 860, 1136, 1208, 2234
Tru9I TTAA 5 cut(s) 660, 860, 1136, 1208, 2234
TscAI CASTG 3 cut(s) 720, 1401, 2359
TseFI GTSAC 5 cut(s) 711, 748, 1595, 2519, 2616
TseI GCWGC 8 cut(s) 154, 454, 631, 1004, 1507, 1919, 2051, 2828
Tsp45I GTSAC 5 cut(s) 711, 748, 1595, 2519, 2616
TspGWI ACGGA 4 cut(s) 55, 177, 464, 2565
TspRI CASTG 3 cut(s) 720, 1401, 2359
Tth111I GACNNNGTC 2 cut(s) 388, 2631
Vha464I CTTAAG 1 cut(s) 1135
VpaK11BI GGWCC 4 cut(s) 899, 1376, 2075, 2633
XapI RAATTY 5 cut(s) 247, 1474, 1523, 1925, 2792
XbaI TCTAGA 1 cut(s) 1454
XhoI CTCGAG 1 cut(s) 926
XmiI GTMKAC 1 cut(s) 2802
XmnI GAANNNNTTC 2 cut(s) 291, 2733
XspI CTAG 3 cut(s) 585, 933, 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.