RLG00000021146

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005671
Physical Location & Seq
Forward (+)
72326101 .. 72331666
5566 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021146

Sequence Viewer

Length: 2841 bp
ATGGAGAAGAGAGAAAAGGTGAAGATGATAGTAGCGGCGGCCCTCGCCTCTCTCTCAGCTCTCTCCGTCTTCTTCCTCTACCGGAGGAGACGCCGAAACTGCCCCCAAACTCACTGTTATTTACGAGACGACCCGAACAAGCCGCAGCACGGATTCAAGCGAGTCTTGGCCGACAATTCCTACTCTCCGTTCAAGCATTTCAGCCCCGCCGACGATGCCGACGACTCTAACGACGGCGGAGCAGATAAGAATTGGAATTCGCATCCTTATGAGGCGGAGATCACGGCGGTGATGGAAAGTCCGCCGATTGAGCTGAAATGTGTGACGGAGAATTTGGATTCGGAAATGAGGGAGGAGTATGTTTGGGTTGAGAATGAGACTCAGTTAAAGGAAATGGTTGAGGTTTTGAGTAAAGAGAAGATCTTTGGGGTTGATACTGAGCAGCATAGCTTAAGGTCATTTTTGGGATTTACTGCTTTGATACAGATTTCGACTGAGAAGGAAGATTATTTGGTGGACACTATTGCTTTACATGACAGTATGAGCCTTTTGCAGCCGGTTTTCGCCGATGCTAGCATCTGCAAGGTGTTCCATGGGGCAGATAGTGATGTTCTCTGGCTGCAAAGGGACTTTCATATTTATGTTGTCAATATGTTTGATACTGCAAAGGCTTGTGAAGTGTTGTCAAAACCGCAGAAATCACTGGCTTATTTACTTGAATCTTACTGTGGAGTCGTCACTAACAAATTGTTTCAGCGTGAAGACTGGAGACAACGCCCTTTGTCAATGGAAATGGTGCAATATGCTCGGACAGATGCACACTATCTACTTTACATTGCAGGCTGTTTACTAGCTGAGCTGGATGTGCTGGATAATGAAAACTCTTGTCCCAATGACAAATTCCGTTTTGTTCTCGAGGCTAGTCGGCGTTCAAACATGATATGTTTGCAACTGTATACGAAAGACATAGAAGCTTTGCCTGGAGAGTCTGCTGCTTCATCAATATGTAGCCGTCAATTAAATGGCCAAGGAGGCATTTCATCAGCATCTTATGAAATCCAGAATTCTGTGAGACGACTTTGTGCATGGAGAGATTTGATGGCTCGTGTACATGATGAGAGCTTGAGATATGTAATATCAGATCAGGCTATTGTTGCCCTTGCAGAAAAAGCTCCAAGTAACCCTGCAGATATGTTAACCGCCATTGCAGAAGCTGATGATAATGTGGATTCAAGTTTCAATTCTGGCCTCTCATCTCCCTCTCCTGTGGTCTGCAGTCATTTGAATGATTTTCATTATCTGCTACAGAACAAAATAGGCAATCCTGATGATCTCTTACCCATGATTCTTCAGAACTGCCTGGGTCAAAAAGGGAGTTGCCCACTGTCTGTCTTCAACTATGCTTTACTGGTTAATTATAATATGAAAGTACCACTGGTTTCTAAACAAAATGGCGTCAGAAATCCAAAGCAAGTCGGTCGAATGGCTTCACGAAAGCTGTTTGTTCAAAAATTTTCCTGCAAATCCCCAGTTTATCATAATTGCAGGATCTATGCAAATGATGGGTGTTTGCTGTGTTACTGCGACCGCAGAAAGCTTGAATGGTATCTTCATCGGAATTTGGCTAAACTTGTTGAGGACAATCCACCTGGAATTATGCTTCTTTTTGAGCCCAAAGGCCGTCCAGAAGATGAAGGCAATGATTTCTACATTCAAAGTAAGAAAAATATGTGTGTTGGCTGCGGTGAAAGAAATCATTACTTACGGTACCGAATTATTCCCTCATGCTACAGAATGCACTTCCCTGAACATTTGAAAAGCCACCGTTCTCATGATATTGTCCTACTATGCGTGGACTGTCATGAAATTGCTCATGCCGCTGCAGAGAAGTATAAAAGAAAGATTGCTGCAGAATTTGGGATTCCACTTTTTGTTCGTAAAGTGGTTGATTCGCAACAAGCTCAATCTTTATCTGGGGCATCTGTGCCTGCAACAAATGTTGAGGAAGCAGGTGTATCTCCTTTACAGTTGCGAACTGCTGCTATGGCTCTTTTGCGCCATGGGTCAACAATGCCATCCAAGCGCCGTGAGGAACTGACAGAGATTGTTATGACATTTTATGGAGGGAGGCAAATCTCTGAGGAAGATTTAGAAAAGGCTTTGCTTGTTGGGATGACTCCTCATGAGAAAAGACGACTTCAGAAGAAGAAAGGGTTTTCTTTTCAACATTCTAGAGGGAGTATTTCTTCAGATGCAGAGGAGAAAATTACTGTTAACATGTCCACATCTGCCACTCCAGATGCTTCAGAAGTTGATACTGTACATGAATCTTGCACCAATGAAGCCAAAGCATCTATGATAAACCAAGATGAAGATGTTTTCACAGTGAAGGATGCTGATTTAGGCAACTCCGCTTTAGATTTGAGGATTGGTGAAAAAGCATCTCCAAGCAATGAGAAGAACATGAGTTCAGAGATAACCAGGGTGTCCGATACAACACTTTTTTCTGTTGTAAATGGTGATGGTATTTATGACGGTAGAAGTACACCAAATGGATCAGTAGACTTCCGTTCTACTTCCAAACGTAATCATAAATCAAAACACTCCCTCTTAGGACATGGTCCACATGGAAGGCAAGTTGTGGATCATCTGTTAAAGGAATCTGGAGAAGATGGTATTAGTCAGTTCTGTCAAAGGTGGAGACAAGTATTTGTTGAAACTGTTCATCCCCGTTTTTTACCTGCCGGCTGGGATATTACACACAGTGGTAGGAGGGATTTTGGAGAATTTAGCGTCTACAATCCTACCAAGAAAGCTTCTGCTGTTACTTCAAGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0000178 GO:0000460 GO:0000785 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006403 GO:0006725 GO:0006807 GO:0007549 GO:0008150 GO:0008152 GO:0008298 GO:0008334 GO:0008408 GO:0009048 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010639 GO:0016070 GO:0016071 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032210 GO:0032211 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035327 GO:0040029 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045934 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051179 GO:0051641 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071025 GO:0071027 GO:0071028 GO:0071029 GO:0071030 GO:0071033 GO:0071034 GO:0071035 GO:0071043 GO:0071044 GO:0071046 GO:0071048 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902494 GO:1904356 GO:1904357 GO:1904872 GO:1905354 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1 HRDC

Protein Analysis

947

Amino Acids

106.55

Weight (kDa)

6.37

Isoelectric Point (pI)

51.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 120 - 287 5.9e-50 3'-5' exonuclease
HRDC PF00570 357 - 400 5.1e-09 HRDC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1419
AarI CACCTGC 1 cut(s) 2000
Acc36I ACCTGC 2 cut(s) 2000, 2749
Acc65I GGTACC 1 cut(s) 1767
AccB1I GGYRCC 1 cut(s) 1767
AccI GTMKAC 3 cut(s) 956, 2562, 2796
AclWI GGATC 3 cut(s) 1556, 2563, 2652
AcoI YGGCCR 2 cut(s) 168, 1024
AcsI RAATTY 8 cut(s) 256, 331, 899, 1063, 1511, 1618, 1913, 2786
AcuI CTGAAG 4 cut(s) 1334, 2183, 2232, 2289
AcyI GRCGYC 2 cut(s) 91, 1455
AdeI CACNNNGTG 1 cut(s) 2765
AfaI GTAC 5 cut(s) 1110, 1431, 1769, 2322, 2545
AfiI CCNNNNNNNGG 1 cut(s) 149
AflII CTTAAG 1 cut(s) 451
AflIII ACRYGT 1 cut(s) 2277
AjnI CCWGG 4 cut(s) 979, 1359, 1648, 2480
AjuI GAANNNNNNNTTGG 2 cut(s) 884, 916
AleI CACNNNNGTG 1 cut(s) 287
Alw26I GTCTC 6 cut(s) 82, 120, 371, 763, 1066, 2695
AlwI GGATC 3 cut(s) 1556, 2563, 2652
AlwNI CAGNNNCTG 1 cut(s) 1214
Ama87I CYCGRG 1 cut(s) 914
AoxI GGCC 5 cut(s) 39, 168, 1024, 1246, 1678
ApeKI GCWGC 9 cut(s) 145, 442, 553, 619, 992, 1740, 1880, 1907, 2039
ApoI RAATTY 8 cut(s) 256, 331, 899, 1063, 1511, 1618, 1913, 2786
ArsI GACNNNNNNTTYG 2 cut(s) 316, 348
Asp700I GAANNNNTTC 2 cut(s) 1486, 2721
Asp718I GGTACC 1 cut(s) 1767
AspLEI GCGC 2 cut(s) 2058, 2085
AspS9I GGNCC 2 cut(s) 40, 2621
AsuHPI GGTGA 5 cut(s) 31, 301, 1757, 2444, 2531
AsuNHI GCTAGC 1 cut(s) 572
AvaI CYCGRG 1 cut(s) 914
AvaII GGWCC 1 cut(s) 2621
BalI TGGCCA 1 cut(s) 1026
BanI GGYRCC 1 cut(s) 1767
BanII GRGCYC 1 cut(s) 1674
BauI CACGAG 1 cut(s) 1104
BbsI GAAGAC 3 cut(s) 61, 768, 1384
BbvI GCAGC 9 cut(s) 157, 454, 565, 606, 979, 1727, 1867, 1894, 2026
BccI CCATC 6 cut(s) 286, 1093, 1556, 2083, 2516, 2666
BceAI ACGGC 5 cut(s) 250, 300, 996, 1665, 2070
BcgI CGANNNNNNTGC 4 cut(s) 788, 822, 1460, 1494
BciT130I CCWGG 4 cut(s) 981, 1361, 1650, 2482
BcoDI GTCTC 6 cut(s) 82, 120, 371, 763, 1066, 2695
BfaI CTAG 4 cut(s) 573, 851, 921, 2232
BfmI CTRYAG 6 cut(s) 1185, 1273, 1304, 1789, 1881, 1908
BfoI RGCGCY 1 cut(s) 2086
BfrI CTTAAG 1 cut(s) 451
BfuAI ACCTGC 2 cut(s) 2000, 2749
BglI GCCNNNNNGGC 1 cut(s) 1032
BglII AGATCT 1 cut(s) 420
BlpI GCTNAGC 1 cut(s) 855
Bme1390I CCNGG 4 cut(s) 981, 1361, 1650, 2482
Bme18I GGWCC 1 cut(s) 2621
BmeT110I CYCGRG 1 cut(s) 914
BmgT120I GGNCC 2 cut(s) 40, 2621
BmiI GGNNCC 1 cut(s) 1769
BmrFI CCNGG 4 cut(s) 981, 1361, 1650, 2482
BmrI ACTGGG 1 cut(s) 1523
BmtI GCTAGC 1 cut(s) 576
BmuI ACTGGG 1 cut(s) 1523
BpiI GAAGAC 3 cut(s) 61, 768, 1384
BpmI CTGGAG 4 cut(s) 787, 1002, 2280, 2685
Bpu1102I GCTNAGC 1 cut(s) 855
BpuEI CTTGAG 1 cut(s) 1144
BsaHI GRCGYC 2 cut(s) 91, 1455
BsaJI CCNNGG 5 cut(s) 592, 1027, 1360, 2059, 2481
BsaWI WCCGGW 1 cut(s) 81
Bsc4I CCNNNNNNNGG 1 cut(s) 149
Bse118I RCCGGY 2 cut(s) 556, 2744
Bse1I ACTGG 5 cut(s) 708, 770, 1413, 1440, 1529
Bse3DI GCAATG 4 cut(s) 834, 1203, 1705, 2458
BseBI CCWGG 4 cut(s) 981, 1361, 1650, 2482
BseDI CCNNGG 5 cut(s) 592, 1027, 1360, 2059, 2481
BseGI GGATG 6 cut(s) 262, 868, 2075, 2178, 2398, 2725
BseLI CCNNNNNNNGG 1 cut(s) 149
BseMI GCAATG 4 cut(s) 834, 1203, 1705, 2458
BseMII CTCAG 6 cut(s) 69, 395, 429, 486, 846, 2130
BseNI ACTGG 5 cut(s) 708, 770, 1413, 1440, 1529
BseRI GAGGAG 4 cut(s) 100, 368, 2169, 2273
BseXI GCAGC 9 cut(s) 157, 454, 565, 606, 979, 1727, 1867, 1894, 2026
BseYI CCCAGC 1 cut(s) 2748
Bsh1285I CGRYCG 2 cut(s) 1480, 1588
BshFI GGCC 5 cut(s) 41, 170, 1026, 1248, 1680
BshNI GGYRCC 1 cut(s) 1767
BsiEI CGRYCG 2 cut(s) 1480, 1588
BsiHKCI CYCGRG 1 cut(s) 914
BsiSI CCGG 3 cut(s) 82, 557, 2745
BslFI GGGAC 2 cut(s) 641, 873
BslI CCNNNNNNNGG 1 cut(s) 149
BsmAI GTCTC 6 cut(s) 82, 120, 371, 763, 1066, 2695
BsmBI CGTCTC 3 cut(s) 82, 120, 1066
BsmFI GGGAC 2 cut(s) 641, 873
BsmI GAATGC 1 cut(s) 1800
BsnI GGCC 5 cut(s) 41, 170, 1026, 1248, 1680
BsoBI CYCGRG 1 cut(s) 914
Bsp1286I GDGCHC 1 cut(s) 1674
Bsp1407I TGTACA 2 cut(s) 1108, 2320
Bsp143I GATC 7 cut(s) 279, 420, 1141, 1330, 1548, 2555, 2644
Bsp1720I GCTNAGC 1 cut(s) 855
Bsp19I CCATGG 2 cut(s) 592, 2059
BspANI GGCC 5 cut(s) 41, 170, 1026, 1248, 1680
BspCNI CTCAG 6 cut(s) 68, 394, 430, 487, 847, 2131
BspHI TCATGA 3 cut(s) 1831, 1861, 2182
BspLI GGNNCC 1 cut(s) 1769
BspMAI CTGCAG 4 cut(s) 1189, 1277, 1885, 1912
BspMI ACCTGC 2 cut(s) 2000, 2749
BspOI GCTAGC 1 cut(s) 576
BspPI GGATC 3 cut(s) 1556, 2563, 2652
BspT107I GGYRCC 1 cut(s) 1767
BspTI CTTAAG 1 cut(s) 451
BsrDI GCAATG 4 cut(s) 834, 1203, 1705, 2458
BsrFI RCCGGY 2 cut(s) 556, 2744
BsrGI TGTACA 2 cut(s) 1108, 2320
BsrI ACTGG 5 cut(s) 708, 770, 1413, 1440, 1529
BssAI RCCGGY 2 cut(s) 556, 2744
BssECI CCNNGG 5 cut(s) 592, 1027, 1360, 2059, 2481
BssMI GATC 7 cut(s) 279, 420, 1141, 1330, 1548, 2555, 2644
BssNAI GTATAC 1 cut(s) 957
BssNI GRCGYC 2 cut(s) 91, 1455
BssSI CACGAG 1 cut(s) 1104
BssT1I CCWWGG 3 cut(s) 592, 1027, 2059
Bst1107I GTATAC 1 cut(s) 957
Bst2BI CACGAG 1 cut(s) 1104
Bst2UI CCWGG 4 cut(s) 981, 1361, 1650, 2482
Bst6I CTCTTC 1 cut(s) 2
BstACI GRCGYC 2 cut(s) 91, 1455
BstAFI CTTAAG 1 cut(s) 451
BstAUI TGTACA 2 cut(s) 1108, 2320
BstC8I GCNNGC 4 cut(s) 574, 841, 1989, 2746
BstDEI CTNAG 7 cut(s) 55, 381, 438, 495, 855, 2139, 2611
BstDSI CCRYGG 2 cut(s) 592, 2059
BstF5I GGATG 6 cut(s) 262, 868, 2075, 2178, 2398, 2725
BstH2I RGCGCY 1 cut(s) 2086
BstHHI GCGC 2 cut(s) 2058, 2085
BstKTI GATC 7 cut(s) 282, 423, 1144, 1333, 1551, 2558, 2647
BstMAI GTCTC 6 cut(s) 82, 120, 371, 763, 1066, 2695
BstMBI GATC 7 cut(s) 279, 420, 1141, 1330, 1548, 2555, 2644
BstMCI CGRYCG 2 cut(s) 1480, 1588
BstNI CCWGG 4 cut(s) 981, 1361, 1650, 2482
BstNSI RCATGY 1 cut(s) 2281
BstSCI CCNGG 4 cut(s) 979, 1359, 1648, 2480
BstSFI CTRYAG 6 cut(s) 1185, 1273, 1304, 1789, 1881, 1908
BstV1I GCAGC 9 cut(s) 157, 454, 565, 606, 979, 1727, 1867, 1894, 2026
BstV2I GAAGAC 3 cut(s) 61, 768, 1384
BstX2I RGATCY 2 cut(s) 420, 1548
BstYI RGATCY 2 cut(s) 420, 1548
BstZ17I GTATAC 1 cut(s) 957
BsuRI GGCC 5 cut(s) 41, 170, 1026, 1248, 1680
BtgI CCRYGG 2 cut(s) 592, 2059
BtsCI GGATG 6 cut(s) 262, 868, 2075, 2178, 2398, 2725
BtsIMutI CAGTG 6 cut(s) 112, 701, 1382, 1433, 2391, 2770
BveI ACCTGC 2 cut(s) 2000, 2749
Cac8I GCNNGC 4 cut(s) 574, 841, 1989, 2746
CaiI CAGNNNCTG 1 cut(s) 1214
CciI TCATGA 3 cut(s) 1831, 1861, 2182
CfoI GCGC 2 cut(s) 2058, 2085
Cfr10I RCCGGY 2 cut(s) 556, 2744
Cfr13I GGNCC 2 cut(s) 40, 2621
CseI GACGC 3 cut(s) 99, 1444, 2782
Csp6I GTAC 5 cut(s) 1109, 1430, 1768, 2321, 2544
CviQI GTAC 5 cut(s) 1109, 1430, 1768, 2321, 2544
DdeI CTNAG 7 cut(s) 55, 381, 438, 495, 855, 2139, 2611
DpnI GATC 7 cut(s) 281, 422, 1143, 1332, 1550, 2557, 2646
DpnII GATC 7 cut(s) 279, 420, 1141, 1330, 1548, 2555, 2644
DraIII CACNNNGTG 1 cut(s) 2765
EaeI YGGCCR 2 cut(s) 168, 1024
Eam1104I CTCTTC 1 cut(s) 2
EarI CTCTTC 1 cut(s) 2
EciI GGCGGA 3 cut(s) 252, 290, 291
Eco130I CCWWGG 3 cut(s) 592, 1027, 2059
Eco24I GRGCYC 1 cut(s) 1674
Eco47I GGWCC 1 cut(s) 2621
Eco57I CTGAAG 4 cut(s) 1334, 2183, 2232, 2289
Eco88I CYCGRG 1 cut(s) 914
EcoRI GAATTC 2 cut(s) 256, 1063
EcoRII CCWGG 4 cut(s) 979, 1359, 1648, 2480
EcoT14I CCWWGG 3 cut(s) 592, 1027, 2059
EcoT38I GRGCYC 1 cut(s) 1674
ErhI CCWWGG 3 cut(s) 592, 1027, 2059
Esp3I CGTCTC 3 cut(s) 82, 120, 1066
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FaqI GGGAC 2 cut(s) 641, 873
FauI CCCGC 1 cut(s) 214
FblI GTMKAC 3 cut(s) 956, 2562, 2796
FokI GGATG 6 cut(s) 249, 875, 2062, 2185, 2405, 2712
FriOI GRGCYC 1 cut(s) 1674
FspBI CTAG 4 cut(s) 573, 851, 921, 2232
GlaI GCGC 2 cut(s) 2057, 2084
GsaI CCCAGC 1 cut(s) 2752
GsuI CTGGAG 4 cut(s) 787, 1002, 2280, 2685
HaeII RGCGCY 1 cut(s) 2086
HaeIII GGCC 5 cut(s) 41, 170, 1026, 1248, 1680
HapII CCGG 3 cut(s) 82, 557, 2745
HgaI GACGC 3 cut(s) 99, 1444, 2782
HhaI GCGC 2 cut(s) 2058, 2085
Hin1I GRCGYC 2 cut(s) 91, 1455
Hin6I GCGC 2 cut(s) 2056, 2083
HinP1I GCGC 2 cut(s) 2056, 2083
HincII GTYRAC 3 cut(s) 1197, 2067, 2275
HindII GTYRAC 3 cut(s) 1197, 2067, 2275
HindIII AAGCTT 3 cut(s) 972, 1595, 2814
HpaI GTTAAC 2 cut(s) 1197, 2275
HpaII CCGG 3 cut(s) 82, 557, 2745
HphI GGTGA 5 cut(s) 31, 301, 1757, 2444, 2531
Hpy99I CGWCG 3 cut(s) 215, 224, 236
HpyAV CCTTC 4 cut(s) 493, 1688, 2383, 2625
HpyCH4IV ACGT 1 cut(s) 2584
HpyF3I CTNAG 7 cut(s) 55, 381, 438, 495, 855, 2139, 2611
HpySE526I ACGT 1 cut(s) 2584
Hsp92I GRCGYC 2 cut(s) 91, 1455
HspAI GCGC 2 cut(s) 2056, 2083
KpnI GGTACC 1 cut(s) 1771
KroI GCCGGC 1 cut(s) 2744
KroNI GCCGGC 1 cut(s) 2746
KspAI GTTAAC 2 cut(s) 1197, 2275
Kzo9I GATC 7 cut(s) 279, 420, 1141, 1330, 1548, 2555, 2644
LmnI GCTCC 2 cut(s) 239, 1177
Lsp1109I GCAGC 9 cut(s) 157, 454, 565, 606, 979, 1727, 1867, 1894, 2026
MaeI CTAG 4 cut(s) 573, 851, 921, 2232
MaeII ACGT 1 cut(s) 2584
MaeIII GTNAC 5 cut(s) 322, 736, 1178, 1577, 2824
MalI GATC 7 cut(s) 281, 422, 1143, 1332, 1550, 2557, 2646
MboI GATC 7 cut(s) 279, 420, 1141, 1330, 1548, 2555, 2644
MflI RGATCY 2 cut(s) 420, 1548
MhlI GDGCHC 1 cut(s) 1674
MlsI TGGCCA 1 cut(s) 1026
MluNI TGGCCA 1 cut(s) 1026
MlyI GAGTC 6 cut(s) 171, 218, 373, 741, 995, 2170
Mox20I TGGCCA 1 cut(s) 1026
MroNI GCCGGC 1 cut(s) 2744
MroXI GAANNNNTTC 2 cut(s) 1486, 2721
MscI TGGCCA 1 cut(s) 1026
MseI TTAA 7 cut(s) 386, 452, 1019, 1196, 1413, 2274, 2654
MslI CAYNNNNRTG 5 cut(s) 267, 287, 639, 1003, 1284
Msp20I TGGCCA 1 cut(s) 1026
MspA1I CMGCKG 1 cut(s) 1880
MspCI CTTAAG 1 cut(s) 451
MspI CCGG 3 cut(s) 82, 557, 2745
MspR9I CCNGG 4 cut(s) 981, 1361, 1650, 2482
Mva1269I GAATGC 1 cut(s) 1800
MvaI CCWGG 4 cut(s) 981, 1361, 1650, 2482
NaeI GCCGGC 1 cut(s) 2746
NcoI CCATGG 2 cut(s) 592, 2059
NdeII GATC 7 cut(s) 279, 420, 1141, 1330, 1548, 2555, 2644
NgoMIV GCCGGC 1 cut(s) 2744
NheI GCTAGC 1 cut(s) 572
NlaIV GGNNCC 1 cut(s) 1769
NmuCI GTSAC 2 cut(s) 322, 736
NspI RCATGY 1 cut(s) 2281
OliI CACNNNNGTG 1 cut(s) 287
PaeR7I CTCGAG 1 cut(s) 914
PagI TCATGA 3 cut(s) 1831, 1861, 2182
PaqCI CACCTGC 1 cut(s) 2000
PciI ACATGT 1 cut(s) 2277
PcsI WCGNNNNNNNCGW 2 cut(s) 219, 228
PctI GAATGC 1 cut(s) 1800
PdiI GCCGGC 1 cut(s) 2746
PdmI GAANNNNTTC 2 cut(s) 1486, 2721
PfeI GAWTC 9 cut(s) 153, 338, 719, 1229, 1345, 1921, 1949, 2327, 2660
PflFI GACNNNGTC 1 cut(s) 2619
PleI GAGTC 6 cut(s) 170, 218, 373, 740, 994, 2170
PpsI GAGTC 6 cut(s) 170, 218, 373, 740, 994, 2170
PscI ACATGT 1 cut(s) 2277
PsiI TTATAA 1 cut(s) 1419
Psp6I CCWGG 4 cut(s) 979, 1359, 1648, 2480
PspFI CCCAGC 1 cut(s) 2748
PspGI CCWGG 4 cut(s) 979, 1359, 1648, 2480
PspN4I GGNNCC 1 cut(s) 1769
PspPI GGNCC 2 cut(s) 40, 2621
PstI CTGCAG 4 cut(s) 1189, 1277, 1885, 1912
PstNI CAGNNNCTG 1 cut(s) 1214
PsuI RGATCY 2 cut(s) 420, 1548
PsyI GACNNNGTC 1 cut(s) 2619
RsaI GTAC 5 cut(s) 1110, 1431, 1769, 2322, 2545
RsaNI GTAC 5 cut(s) 1109, 1430, 1768, 2321, 2544
RseI CAYNNNNRTG 5 cut(s) 267, 287, 639, 1003, 1284
SaqAI TTAA 7 cut(s) 386, 452, 1019, 1196, 1413, 2274, 2654
Sau3AI GATC 7 cut(s) 279, 420, 1141, 1330, 1548, 2555, 2644
Sau96I GGNCC 2 cut(s) 40, 2621
SchI GAGTC 6 cut(s) 171, 218, 373, 741, 995, 2170
ScrFI CCNGG 4 cut(s) 981, 1361, 1650, 2482
SduI GDGCHC 1 cut(s) 1674
SfcI CTRYAG 6 cut(s) 1185, 1273, 1304, 1789, 1881, 1908
Sfr274I CTCGAG 1 cut(s) 914
SinI GGWCC 1 cut(s) 2621
SlaI CTCGAG 1 cut(s) 914
SmiMI CAYNNNNRTG 5 cut(s) 267, 287, 639, 1003, 1284
SmlI CTYRAG 3 cut(s) 451, 914, 1123
SmoI CTYRAG 3 cut(s) 451, 914, 1123
SspMI CTAG 4 cut(s) 573, 851, 921, 2232
StyD4I CCNGG 4 cut(s) 979, 1359, 1648, 2480
StyI CCWWGG 3 cut(s) 592, 1027, 2059
TaiI ACGT 1 cut(s) 2587
TaqI TCGA 3 cut(s) 491, 915, 1480
TaqII GACCGA 1 cut(s) 1466
TatI WGTACW 3 cut(s) 1108, 2320, 2543
TauI GCSGC 4 cut(s) 38, 41, 145, 1880
TfiI GAWTC 9 cut(s) 153, 338, 719, 1229, 1345, 1921, 1949, 2327, 2660
Tru1I TTAA 7 cut(s) 386, 452, 1019, 1196, 1413, 2274, 2654
Tru9I TTAA 7 cut(s) 386, 452, 1019, 1196, 1413, 2274, 2654
TscAI CASTG 6 cut(s) 119, 708, 1389, 1440, 2391, 2770
TseFI GTSAC 2 cut(s) 322, 736
TseI GCWGC 9 cut(s) 145, 442, 553, 619, 992, 1740, 1880, 1907, 2039
Tsp45I GTSAC 2 cut(s) 322, 736
TspGWI ACGGA 6 cut(s) 55, 165, 177, 341, 893, 2558
TspRI CASTG 6 cut(s) 119, 708, 1389, 1440, 2391, 2770
Tth111I GACNNNGTC 1 cut(s) 2619
Vha464I CTTAAG 1 cut(s) 451
VpaK11BI GGWCC 1 cut(s) 2621
XapI RAATTY 8 cut(s) 256, 331, 899, 1063, 1511, 1618, 1913, 2786
XbaI TCTAGA 1 cut(s) 2231
XceI RCATGY 1 cut(s) 2281
XhoI CTCGAG 1 cut(s) 914
XmiI GTMKAC 3 cut(s) 956, 2562, 2796
XmnI GAANNNNTTC 2 cut(s) 1486, 2721
XspI CTAG 4 cut(s) 573, 851, 921, 2232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.