Rh2BG554400

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005671
Physical Location & Seq
Forward (+)
76337083 .. 76342239
5157 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG554400.1

Sequence Viewer

Length: 2631 bp
ATGGAGAATGTGATGGTGAATTTGGTGATAGGTAAGAATTGGAATTCGCATCCTTATGAGGCGGAGATCACGGCGGTGATGGAAAGTCCGCCGATTGAGCTGAAATGTGTGACGGAGAAGAATTTGGATTCGGAAATGAGGGAGGAGTATGTTTGGGTAGAGAATGAGACTCAGTTGAAGGAAATGGTTGAGGTTTTGAGTAAAGAGAAGATCTTTGGGGTTGATACTGAGCAGCATAGCTTAAGGTCATTTTTGGGATTTACTGCTTTGATACAGATTTCGACTGAGAAGGAAGATTATTTGCTGGACGCTATTGCTTTACATGACAGTATGAGCCTTTTGCAGCCGGTTTTTGCCGATGCTAGTATCTGCAAGGTGTTCCATGGGGCAGATAGTGATGTTCTCTGGCTGCAAAGGGACTTTCATATTTATGTCGTCAATATGTTTGATACTGCAAAGGCTTGTGAAGTGTTGTCAAAACCGCAGAAATCACTGGCTTATTTACTTGAATCTTACTGTGGAGTTGTCACTAACAAATTGTTTCAGCGTGAAGACTGGAGACAACGCCCTTTGTCAATGGAAATGGTGCAATATGCTCGGACAGATGCACACTATCTCCTTTACATTGCAGGCCGTTTACTAGCTGAGCTGGATGTGCTGGATAATGAAAACTCTTGTCCCAATGACAAATTCCGTTTTGTTCTCGAGGCTAGTCGGCGTTCAAACATGATATGTTTGCAACTGTATACAAAAGACGTAGAAGCTTCGCCTGGAGAGTCTGCTGCTTCATCAATATTTAGCCGTCATTTAAATGGCCAAGGAGGCATTTCATCAGCATCTTATGAAATCCAGAATTCTGTGAGACGACTTTGTGCATGGAGAGATTTGATGGCTCGTGTACATGATGAGAGCTTGAGATATGTAATATCAGATCAGGCTATTGTTGCCCTTGCAGAAAAAGCTCCAACTAACCCTGCAGACATGTTAACCACCATTGCAGAAGCTGATGATAATGTGGATTCAAGTTTCAATTCTGGCCTCTCATCTCCCTCTCCTGTGGTCTGCAGTCATTTGAATGATTTTTATTATCTGCTCCAGAAGAAAGTAGGCAATCCTGATGATCTCTTACCCATGATTCTTCAGAACTGCCTGGGTCAAAAAGGGAGTTGCCCACTGTCTGTCTTCAACTATGCTTTACTGGTTAATTATAATATGAAAGTACCACTGGTTTCTAAACAAAATGGGGTCAGAAATCCAAAGCAAGTCGGTCGAATGGCTTCGCGAAAGCTGTTTGTTCAAAAATTTTCCTGCAAATCCCCAGTTTATCATAATTGCAGGATCTATGCAAATGATGGGCGTTTGCTGTGTTACTGTGACCGCAGAAAGCTTGAATGGTATCTTCATCGGAATTTGGCTAAACTTGTTGAGGACAATCCACCTGGAATTATGCTTCTTTTTGAGCCCAAAGGCCGTCCAGAAGATGAAGGCAATGATTTCTACATTCAAAGTAAGAAAAATATGTGTGTTGGCTGCGGTGAAAGAAATCATTACTTACGGTACCGAATTATTCCCTCATGCTACAGAATGCACTTCCCTGAACATTTGAAAAGCCACCGTTCTCATGATATTGTCCTACTATGCGTGGACTGTCACGAAATTGCTCATGCCGCTGCAGAGAAGTATAAAAGAAAGATTGCTGCAGAATTTGGGATTCCACTTTTTGTTCGTAAAGTGGTTGATTCACAACAAGCTCAATCTTTATCTGGGGCATCTGTGCCTGCAACAAATGTTGAGGAAGCAGGTGTATCTCCTTTACAGTTGCGAACTGCTGCTATGGCCCTTTTGCGCCATGGATCAACAATGCCATCCAAGCGCCGTGAGGAACTGACAGAGATTGTTATGACATTTTATGGAGGGAGGCAAATCTCTGAGGAAGATTTAGAAAAGGCTTTGCTTGTTGGGATGACTCCTCATGAGAAAAGACGACTTCAGAAGAAGAAAGGGTTTTCTTTTCAACATTCTAGAGGGAGTATTTCTTCAGATGCAGAGGAGAAAATTACTGTTAACATGTCCACATCTGCCACTCCAGATGCTTCAGAAGTTGATACTGTACATGAATCTTGCACCAATGAAGCCAAAGCATCTATGATAAACCAAGATGAAGATGTTTTCACAGTGAAGGATGCTGATTTAGGCAACTCTGCTTTAGATTTGAGGATTGGGGAAAAAGCATCTCCAAGCAATGAGAAGAACATGAGTTCAGAGATAACCAGAGTGTCCGATACTACACTTGTTTCTGTTGTAAATGGTGATGGTATTTATGACCGTAGAAGTACACCAAATGGATCAGTAGACTTCCGTTCTACTTCCAAACGTAATCATAAATCAAAACACTCCCTCTTAGGACATGGTCCACATGGAAGGCAAGTTGTGGATCATCTGTTAAAGGAATCTGGAGAAGATGGTATTAATCAGTTCTGTCAAAGGTGGAGACAAGTATTTGTTGAAACTGTTCATCCCCGTTTTTTACCTGCCGGCTGGGATATTACACACAGTGGTAGGAGGGATTTTGGAGAATTTAGCGTCTACAATCCTACCAAGAAAGCTTCTGCTGTTACGTCAAGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0000178 GO:0000460 GO:0000785 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006403 GO:0006725 GO:0006807 GO:0007549 GO:0008150 GO:0008152 GO:0008298 GO:0008334 GO:0008408 GO:0009048 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010639 GO:0016070 GO:0016071 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032210 GO:0032211 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035327 GO:0040029 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045934 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051179 GO:0051641 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071025 GO:0071027 GO:0071028 GO:0071029 GO:0071030 GO:0071033 GO:0071034 GO:0071035 GO:0071043 GO:0071044 GO:0071046 GO:0071048 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902494 GO:1904356 GO:1904357 GO:1904872 GO:1905354 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1 HRDC

Protein Analysis

876

Amino Acids

98.81

Weight (kDa)

6.38

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 50 - 217 5.3e-50 3'-5' exonuclease
HRDC PF00570 287 - 330 1.2e-09 HRDC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1209
AarI CACCTGC 1 cut(s) 1790
Acc36I ACCTGC 2 cut(s) 1790, 2539
Acc65I GGTACC 1 cut(s) 1557
AccB1I GGYRCC 1 cut(s) 1557
AccI GTMKAC 3 cut(s) 746, 2352, 2586
AccII CGCG 1 cut(s) 1282
AciI CCGC 7 cut(s) 62, 74, 89, 482, 1378, 1533, 1668
AclWI GGATC 4 cut(s) 1346, 1861, 2353, 2442
AcoI YGGCCR 1 cut(s) 814
AcsI RAATTY 9 cut(s) 19, 43, 121, 689, 853, 1301, 1408, 1703, 2576
AcuI CTGAAG 4 cut(s) 1124, 1973, 2022, 2079
AdeI CACNNNGTG 1 cut(s) 2555
AfaI GTAC 5 cut(s) 900, 1221, 1559, 2112, 2335
AflII CTTAAG 1 cut(s) 241
AflIII ACRYGT 2 cut(s) 981, 2067
AjnI CCWGG 3 cut(s) 769, 1149, 1438
AjuI GAANNNNNNNTTGG 2 cut(s) 674, 706
AleI CACNNNNGTG 1 cut(s) 74
Alw26I GTCTC 4 cut(s) 161, 553, 856, 2485
AlwI GGATC 4 cut(s) 1346, 1861, 2353, 2442
AlwNI CAGNNNCTG 1 cut(s) 1004
Ama87I CYCGRG 1 cut(s) 704
AoxI GGCC 5 cut(s) 631, 814, 1036, 1468, 1836
ApeKI GCWGC 8 cut(s) 232, 343, 409, 782, 1530, 1670, 1697, 1829
ApoI RAATTY 9 cut(s) 19, 43, 121, 689, 853, 1301, 1408, 1703, 2576
AseI ATTAAT 1 cut(s) 2469
Asp700I GAANNNNTTC 2 cut(s) 1276, 2511
Asp718I GGTACC 1 cut(s) 1557
AspLEI GCGC 2 cut(s) 1848, 1875
AspS9I GGNCC 2 cut(s) 1837, 2411
AsuHPI GGTGA 5 cut(s) 28, 37, 88, 1547, 2321
AvaI CYCGRG 1 cut(s) 704
AvaII GGWCC 1 cut(s) 2411
BalI TGGCCA 1 cut(s) 816
BanI GGYRCC 1 cut(s) 1557
BanII GRGCYC 1 cut(s) 1464
BauI CACGAG 1 cut(s) 894
BbsI GAAGAC 2 cut(s) 558, 1174
BbvI GCAGC 8 cut(s) 244, 355, 396, 769, 1517, 1657, 1684, 1816
BccI CCATC 7 cut(s) 7, 73, 883, 1346, 1873, 2306, 2456
BceAI ACGGC 5 cut(s) 87, 618, 786, 1455, 1860
BcgI CGANNNNNNTGC 4 cut(s) 578, 612, 1250, 1284
BciT130I CCWGG 3 cut(s) 771, 1151, 1440
BcoDI GTCTC 4 cut(s) 161, 553, 856, 2485
BfaI CTAG 4 cut(s) 363, 641, 711, 2022
BfmI CTRYAG 5 cut(s) 975, 1063, 1579, 1671, 1698
BfoI RGCGCY 1 cut(s) 1876
BfrI CTTAAG 1 cut(s) 241
BfuAI ACCTGC 2 cut(s) 1790, 2539
BglI GCCNNNNNGGC 1 cut(s) 822
BglII AGATCT 1 cut(s) 210
BisI GCNGC 9 cut(s) 233, 344, 410, 783, 1531, 1668, 1671, 1698, 1830
BlpI GCTNAGC 1 cut(s) 645
BlsI GCNGC 9 cut(s) 234, 345, 411, 784, 1532, 1669, 1672, 1699, 1831
Bme1390I CCNGG 3 cut(s) 771, 1151, 1440
Bme18I GGWCC 1 cut(s) 2411
BmeT110I CYCGRG 1 cut(s) 704
BmgT120I GGNCC 2 cut(s) 1837, 2411
BmiI GGNNCC 1 cut(s) 1559
BmrFI CCNGG 3 cut(s) 771, 1151, 1440
BmrI ACTGGG 1 cut(s) 1313
BmuI ACTGGG 1 cut(s) 1313
BpiI GAAGAC 2 cut(s) 558, 1174
BpmI CTGGAG 5 cut(s) 577, 792, 1079, 2070, 2475
Bpu1102I GCTNAGC 1 cut(s) 645
BpuEI CTTGAG 1 cut(s) 934
BsaJI CCNNGG 4 cut(s) 382, 817, 1150, 1849
BsaXI ACNNNNNCTCC 2 cut(s) 600, 630
Bse118I RCCGGY 2 cut(s) 346, 2534
Bse1I ACTGG 5 cut(s) 498, 560, 1203, 1230, 1319
Bse3DI GCAATG 4 cut(s) 624, 993, 1495, 2248
BseBI CCWGG 3 cut(s) 771, 1151, 1440
BseDI CCNNGG 4 cut(s) 382, 817, 1150, 1849
BseGI GGATG 6 cut(s) 49, 658, 1865, 1968, 2188, 2515
BseMI GCAATG 4 cut(s) 624, 993, 1495, 2248
BseMII CTCAG 5 cut(s) 185, 219, 276, 636, 1920
BseNI ACTGG 5 cut(s) 498, 560, 1203, 1230, 1319
BseRI GAGGAG 3 cut(s) 158, 1959, 2063
BseXI GCAGC 8 cut(s) 244, 355, 396, 769, 1517, 1657, 1684, 1816
BseYI CCCAGC 1 cut(s) 2538
Bsh1236I CGCG 1 cut(s) 1282
Bsh1285I CGRYCG 1 cut(s) 1270
BshFI GGCC 5 cut(s) 633, 816, 1038, 1470, 1838
BshNI GGYRCC 1 cut(s) 1557
BsiEI CGRYCG 1 cut(s) 1270
BsiHKCI CYCGRG 1 cut(s) 704
BsiSI CCGG 2 cut(s) 347, 2535
BslFI GGGAC 2 cut(s) 431, 663
BsmAI GTCTC 4 cut(s) 161, 553, 856, 2485
BsmBI CGTCTC 1 cut(s) 856
BsmFI GGGAC 2 cut(s) 431, 663
BsmI GAATGC 1 cut(s) 1590
BsnI GGCC 5 cut(s) 633, 816, 1038, 1470, 1838
BsoBI CYCGRG 1 cut(s) 704
Bsp1286I GDGCHC 1 cut(s) 1464
Bsp1407I TGTACA 2 cut(s) 898, 2110
Bsp143I GATC 8 cut(s) 66, 210, 931, 1120, 1338, 1853, 2345, 2434
Bsp1720I GCTNAGC 1 cut(s) 645
Bsp19I CCATGG 2 cut(s) 382, 1849
Bsp68I TCGCGA 1 cut(s) 1282
BspACI CCGC 7 cut(s) 62, 74, 89, 482, 1378, 1533, 1668
BspANI GGCC 5 cut(s) 633, 816, 1038, 1470, 1838
BspCNI CTCAG 5 cut(s) 184, 220, 277, 637, 1921
BspFNI CGCG 1 cut(s) 1282
BspHI TCATGA 2 cut(s) 1621, 1972
BspLI GGNNCC 1 cut(s) 1559
BspMAI CTGCAG 4 cut(s) 979, 1067, 1675, 1702
BspMI ACCTGC 2 cut(s) 1790, 2539
BspPI GGATC 4 cut(s) 1346, 1861, 2353, 2442
BspT107I GGYRCC 1 cut(s) 1557
BspTI CTTAAG 1 cut(s) 241
BsrDI GCAATG 4 cut(s) 624, 993, 1495, 2248
BsrFI RCCGGY 2 cut(s) 346, 2534
BsrGI TGTACA 2 cut(s) 898, 2110
BsrI ACTGG 5 cut(s) 498, 560, 1203, 1230, 1319
BssAI RCCGGY 2 cut(s) 346, 2534
BssECI CCNNGG 4 cut(s) 382, 817, 1150, 1849
BssMI GATC 8 cut(s) 66, 210, 931, 1120, 1338, 1853, 2345, 2434
BssNAI GTATAC 1 cut(s) 747
BssSI CACGAG 1 cut(s) 894
BssT1I CCWWGG 3 cut(s) 382, 817, 1849
Bst1107I GTATAC 1 cut(s) 747
Bst2BI CACGAG 1 cut(s) 894
Bst2UI CCWGG 3 cut(s) 771, 1151, 1440
BstAFI CTTAAG 1 cut(s) 241
BstAUI TGTACA 2 cut(s) 898, 2110
BstC8I GCNNGC 3 cut(s) 631, 1779, 2536
BstDEI CTNAG 6 cut(s) 171, 228, 285, 645, 1929, 2401
BstDSI CCRYGG 2 cut(s) 382, 1849
BstF5I GGATG 6 cut(s) 49, 658, 1865, 1968, 2188, 2515
BstFNI CGCG 1 cut(s) 1282
BstH2I RGCGCY 1 cut(s) 1876
BstHHI GCGC 2 cut(s) 1848, 1875
BstKTI GATC 8 cut(s) 69, 213, 934, 1123, 1341, 1856, 2348, 2437
BstMAI GTCTC 4 cut(s) 161, 553, 856, 2485
BstMBI GATC 8 cut(s) 66, 210, 931, 1120, 1338, 1853, 2345, 2434
BstMCI CGRYCG 1 cut(s) 1270
BstMWI GCNNNNNNNGC 8 cut(s) 97, 655, 822, 944, 959, 1667, 1835, 1870
BstNI CCWGG 3 cut(s) 771, 1151, 1440
BstNSI RCATGY 2 cut(s) 985, 2071
BstSCI CCNGG 3 cut(s) 769, 1149, 1438
BstSFI CTRYAG 5 cut(s) 975, 1063, 1579, 1671, 1698
BstUI CGCG 1 cut(s) 1282
BstV1I GCAGC 8 cut(s) 244, 355, 396, 769, 1517, 1657, 1684, 1816
BstV2I GAAGAC 2 cut(s) 558, 1174
BstX2I RGATCY 2 cut(s) 210, 1338
BstYI RGATCY 2 cut(s) 210, 1338
BstZ17I GTATAC 1 cut(s) 747
BsuRI GGCC 5 cut(s) 633, 816, 1038, 1470, 1838
BtgI CCRYGG 2 cut(s) 382, 1849
BtsCI GGATG 6 cut(s) 49, 658, 1865, 1968, 2188, 2515
BtsIMutI CAGTG 5 cut(s) 491, 1172, 1223, 2181, 2560
BtuMI TCGCGA 1 cut(s) 1282
BveI ACCTGC 2 cut(s) 1790, 2539
Cac8I GCNNGC 3 cut(s) 631, 1779, 2536
CaiI CAGNNNCTG 1 cut(s) 1004
CciI TCATGA 2 cut(s) 1621, 1972
CfoI GCGC 2 cut(s) 1848, 1875
Cfr10I RCCGGY 2 cut(s) 346, 2534
Cfr13I GGNCC 2 cut(s) 1837, 2411
CseI GACGC 2 cut(s) 317, 2572
Csp6I GTAC 5 cut(s) 899, 1220, 1558, 2111, 2334
CviQI GTAC 5 cut(s) 899, 1220, 1558, 2111, 2334
DdeI CTNAG 6 cut(s) 171, 228, 285, 645, 1929, 2401
DpnI GATC 8 cut(s) 68, 212, 933, 1122, 1340, 1855, 2347, 2436
DpnII GATC 8 cut(s) 66, 210, 931, 1120, 1338, 1853, 2345, 2434
DraI TTTAAA 1 cut(s) 810
DraIII CACNNNGTG 1 cut(s) 2555
EaeI YGGCCR 1 cut(s) 814
EciI GGCGGA 2 cut(s) 77, 78
Eco130I CCWWGG 3 cut(s) 382, 817, 1849
Eco24I GRGCYC 1 cut(s) 1464
Eco47I GGWCC 1 cut(s) 2411
Eco57I CTGAAG 4 cut(s) 1124, 1973, 2022, 2079
Eco88I CYCGRG 1 cut(s) 704
EcoRI GAATTC 2 cut(s) 43, 853
EcoRII CCWGG 3 cut(s) 769, 1149, 1438
EcoT14I CCWWGG 3 cut(s) 382, 817, 1849
EcoT38I GRGCYC 1 cut(s) 1464
ErhI CCWWGG 3 cut(s) 382, 817, 1849
Esp3I CGTCTC 1 cut(s) 856
FaqI GGGAC 2 cut(s) 431, 663
FblI GTMKAC 3 cut(s) 746, 2352, 2586
Fnu4HI GCNGC 9 cut(s) 233, 344, 410, 783, 1531, 1668, 1671, 1698, 1830
FokI GGATG 6 cut(s) 36, 665, 1852, 1975, 2195, 2502
FriOI GRGCYC 1 cut(s) 1464
Fsp4HI GCNGC 9 cut(s) 233, 344, 410, 783, 1531, 1668, 1671, 1698, 1830
FspBI CTAG 4 cut(s) 363, 641, 711, 2022
GlaI GCGC 2 cut(s) 1847, 1874
GluI GCNGC 9 cut(s) 233, 344, 410, 783, 1531, 1668, 1671, 1698, 1830
GsaI CCCAGC 1 cut(s) 2542
GsuI CTGGAG 5 cut(s) 577, 792, 1079, 2070, 2475
HaeII RGCGCY 1 cut(s) 1876
HaeIII GGCC 5 cut(s) 633, 816, 1038, 1470, 1838
HapII CCGG 2 cut(s) 347, 2535
HgaI GACGC 2 cut(s) 317, 2572
HhaI GCGC 2 cut(s) 1848, 1875
Hin6I GCGC 2 cut(s) 1846, 1873
HinP1I GCGC 2 cut(s) 1846, 1873
HincII GTYRAC 2 cut(s) 987, 2065
HindII GTYRAC 2 cut(s) 987, 2065
HindIII AAGCTT 3 cut(s) 762, 1385, 2604
HpaI GTTAAC 2 cut(s) 987, 2065
HpaII CCGG 2 cut(s) 347, 2535
HphI GGTGA 5 cut(s) 28, 37, 88, 1547, 2321
HpyAV CCTTC 5 cut(s) 172, 283, 1478, 2173, 2415
HpyCH4IV ACGT 3 cut(s) 756, 2374, 2618
HpyF10VI GCNNNNNNNGC 8 cut(s) 97, 655, 822, 944, 959, 1667, 1835, 1870
HpyF3I CTNAG 6 cut(s) 171, 228, 285, 645, 1929, 2401
HpySE526I ACGT 3 cut(s) 756, 2374, 2618
HspAI GCGC 2 cut(s) 1846, 1873
KpnI GGTACC 1 cut(s) 1561
KroI GCCGGC 1 cut(s) 2534
KroNI GCCGGC 1 cut(s) 2536
KspAI GTTAAC 2 cut(s) 987, 2065
Kzo9I GATC 8 cut(s) 66, 210, 931, 1120, 1338, 1853, 2345, 2434
LmnI GCTCC 2 cut(s) 967, 1098
Lsp1109I GCAGC 8 cut(s) 244, 355, 396, 769, 1517, 1657, 1684, 1816
MaeI CTAG 4 cut(s) 363, 641, 711, 2022
MaeII ACGT 3 cut(s) 756, 2374, 2618
MaeIII GTNAC 6 cut(s) 109, 526, 1367, 1373, 1649, 2614
MalI GATC 8 cut(s) 68, 212, 933, 1122, 1340, 1855, 2347, 2436
MboI GATC 8 cut(s) 66, 210, 931, 1120, 1338, 1853, 2345, 2434
MflI RGATCY 2 cut(s) 210, 1338
MhlI GDGCHC 1 cut(s) 1464
MlsI TGGCCA 1 cut(s) 816
MluNI TGGCCA 1 cut(s) 816
MlyI GAGTC 3 cut(s) 163, 785, 1960
MmeI TCCRAC 1 cut(s) 989
Mox20I TGGCCA 1 cut(s) 816
MroNI GCCGGC 1 cut(s) 2534
MroXI GAANNNNTTC 2 cut(s) 1276, 2511
MscI TGGCCA 1 cut(s) 816
MseI TTAA 7 cut(s) 242, 809, 986, 1203, 2064, 2444, 2469
MslI CAYNNNNRTG 5 cut(s) 54, 74, 429, 810, 1074
Msp20I TGGCCA 1 cut(s) 816
MspA1I CMGCKG 1 cut(s) 1670
MspCI CTTAAG 1 cut(s) 241
MspI CCGG 2 cut(s) 347, 2535
MspR9I CCNGG 3 cut(s) 771, 1151, 1440
Mva1269I GAATGC 1 cut(s) 1590
MvaI CCWGG 3 cut(s) 771, 1151, 1440
MvnI CGCG 1 cut(s) 1282
MwoI GCNNNNNNNGC 8 cut(s) 97, 655, 822, 944, 959, 1667, 1835, 1870
NaeI GCCGGC 1 cut(s) 2536
NcoI CCATGG 2 cut(s) 382, 1849
NdeII GATC 8 cut(s) 66, 210, 931, 1120, 1338, 1853, 2345, 2434
NgoMIV GCCGGC 1 cut(s) 2534
NlaIV GGNNCC 1 cut(s) 1559
NmuCI GTSAC 4 cut(s) 109, 526, 1373, 1649
NruI TCGCGA 1 cut(s) 1282
NspI RCATGY 2 cut(s) 985, 2071
OliI CACNNNNGTG 1 cut(s) 74
PaeR7I CTCGAG 1 cut(s) 704
PagI TCATGA 2 cut(s) 1621, 1972
PaqCI CACCTGC 1 cut(s) 1790
PciI ACATGT 2 cut(s) 981, 2067
PctI GAATGC 1 cut(s) 1590
PdiI GCCGGC 1 cut(s) 2536
PdmI GAANNNNTTC 2 cut(s) 1276, 2511
PfeI GAWTC 8 cut(s) 128, 509, 1019, 1135, 1711, 1739, 2117, 2450
PflFI GACNNNGTC 1 cut(s) 2409
PkrI GCNGC 9 cut(s) 234, 345, 411, 784, 1532, 1669, 1672, 1699, 1831
PleI GAGTC 3 cut(s) 163, 784, 1960
PpsI GAGTC 3 cut(s) 163, 784, 1960
PscI ACATGT 2 cut(s) 981, 2067
PshBI ATTAAT 1 cut(s) 2469
PsiI TTATAA 1 cut(s) 1209
Psp6I CCWGG 3 cut(s) 769, 1149, 1438
PspFI CCCAGC 1 cut(s) 2538
PspGI CCWGG 3 cut(s) 769, 1149, 1438
PspN4I GGNNCC 1 cut(s) 1559
PspPI GGNCC 2 cut(s) 1837, 2411
PstI CTGCAG 4 cut(s) 979, 1067, 1675, 1702
PstNI CAGNNNCTG 1 cut(s) 1004
PsuI RGATCY 2 cut(s) 210, 1338
PsyI GACNNNGTC 1 cut(s) 2409
RruI TCGCGA 1 cut(s) 1282
RsaI GTAC 5 cut(s) 900, 1221, 1559, 2112, 2335
RsaNI GTAC 5 cut(s) 899, 1220, 1558, 2111, 2334
RseI CAYNNNNRTG 5 cut(s) 54, 74, 429, 810, 1074
SaqAI TTAA 7 cut(s) 242, 809, 986, 1203, 2064, 2444, 2469
SatI GCNGC 9 cut(s) 233, 344, 410, 783, 1531, 1668, 1671, 1698, 1830
Sau3AI GATC 8 cut(s) 66, 210, 931, 1120, 1338, 1853, 2345, 2434
Sau96I GGNCC 2 cut(s) 1837, 2411
SchI GAGTC 3 cut(s) 163, 785, 1960
ScrFI CCNGG 3 cut(s) 771, 1151, 1440
SduI GDGCHC 1 cut(s) 1464
SfcI CTRYAG 5 cut(s) 975, 1063, 1579, 1671, 1698
Sfr274I CTCGAG 1 cut(s) 704
SinI GGWCC 1 cut(s) 2411
SlaI CTCGAG 1 cut(s) 704
SmiI ATTTAAAT 1 cut(s) 810
SmiMI CAYNNNNRTG 5 cut(s) 54, 74, 429, 810, 1074
SmlI CTYRAG 3 cut(s) 241, 704, 913
SmoI CTYRAG 3 cut(s) 241, 704, 913
SsiI CCGC 7 cut(s) 62, 74, 89, 482, 1378, 1533, 1668
SspI AATATT 1 cut(s) 795
SspMI CTAG 4 cut(s) 363, 641, 711, 2022
StyD4I CCNGG 3 cut(s) 769, 1149, 1438
StyI CCWWGG 3 cut(s) 382, 817, 1849
SwaI ATTTAAAT 1 cut(s) 810
TaiI ACGT 3 cut(s) 759, 2377, 2621
TaqI TCGA 3 cut(s) 281, 705, 1270
TaqII GACCGA 1 cut(s) 1256
TatI WGTACW 3 cut(s) 898, 2110, 2333
TauI GCSGC 1 cut(s) 1670
TfiI GAWTC 8 cut(s) 128, 509, 1019, 1135, 1711, 1739, 2117, 2450
Tru1I TTAA 7 cut(s) 242, 809, 986, 1203, 2064, 2444, 2469
Tru9I TTAA 7 cut(s) 242, 809, 986, 1203, 2064, 2444, 2469
TscAI CASTG 5 cut(s) 498, 1179, 1230, 2181, 2560
TseFI GTSAC 4 cut(s) 109, 526, 1373, 1649
TseI GCWGC 8 cut(s) 232, 343, 409, 782, 1530, 1670, 1697, 1829
Tsp45I GTSAC 4 cut(s) 109, 526, 1373, 1649
TspGWI ACGGA 3 cut(s) 128, 683, 2348
TspRI CASTG 5 cut(s) 498, 1179, 1230, 2181, 2560
Tth111I GACNNNGTC 1 cut(s) 2409
Vha464I CTTAAG 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 2411
VspI ATTAAT 1 cut(s) 2469
XapI RAATTY 9 cut(s) 19, 43, 121, 689, 853, 1301, 1408, 1703, 2576
XbaI TCTAGA 1 cut(s) 2021
XceI RCATGY 2 cut(s) 985, 2071
XhoI CTCGAG 1 cut(s) 704
XmiI GTMKAC 3 cut(s) 746, 2352, 2586
XmnI GAANNNNTTC 2 cut(s) 1276, 2511
XspI CTAG 4 cut(s) 363, 641, 711, 2022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.