Prupe.3G217000_v2.0.a1

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0005671
Physical Location & Seq
Forward (+)
21917016 .. 21922289
5274 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G217000.1

Sequence Viewer

Length: 2772 bp
ATGGAGAAGAAAGCCAAACAGCTGAAGATACTAGTAGTTGCCCTCGCTTCTTTGACAGCTCTTTCAGTCTTCTTCCTCGGGCGAGAACGAAGACGCCGAAAGCAGAAGTGCCCCCAAAGTCGGTGTTATTTACGAGCCGACCACACCAAGCCACAGTGCGGATTCAAGCGTGTCTTGGCTGATAATACATACTCTCCGTTCAAGCATTTAAACCTCCTCAACGACCCTCAAGAAGATAAGACTTGGAATTCGCATCCTTACGAGGCGGAAGTCACTGCCTTGATGGAAAGTCCTCGGCTTGAGCTTCAGTGCATTACGGTGAAGAATGTGGATTTGAAAAAATTGAGTGAGTCGTACGTTTGGGTTGAGACTGAGTCGCAGTTGAAGGAACTCGTTGAGGTTTTAAGTAAAGAGAAGGTCTTTGGCGTTGATACTGAGCAGCATAGCTTAAGATCCTTTTTGGGTTTCACAGCTTTAGTGCAGATTTCTACTGAGAAAGAAGATTATTTGGTGGACACTATTGCTCTGCGTGATTGCATGAGCCTTCTTCGGCCAGTTTTTGCGGATGCTAGTATCTGTAAGGTGTTCCATGGGGCAGATAGTGATGTTCTCTGGCTACAAAGGGATTTCCATATTTATGTTGTTAATCTCTTCGATACTGCAAAGGCTTGCGAAGTGTTGTCAAAATCACAGAAATCACTGGCTTATTTACTTGAATCTTACTGTGGAGTAGTCACAAACAAATTGTTACAGCGCGAAGACTGGAGGCAACGACCCTTGTCAACAGAAATGGTGCAATATGCTCGAACAGATGCACACTATTTACTATATATTTCACGCTGTTTAATTGCTGAGCTGGAAATACTGGATAATGAAAACTCCTGTTCCAATGACAAATTCCATTTTGTTCTCGAGGCTAGTCGGCGTTCAAACTTGATGTGTTTGCAACTCTACACTAAAGAGATAGAAGCTTCTCCTGGAGAGTCTGCTGCATCATCAATTTTTAGCCGCCATTTGAACGGACGAAGAGGAATTTCATCAGTTTCTTGTGAAATCCAGGGTACTGTGAGCAAATTATGCGCATGGAGAGATTTAATGGCTCGTGTACATGATGAGAGCTTGAGATATGTAATATCAGATCAAGCTATTGTTGCCCTTGCAGATAAAGCTCCAACGACCCCTACAGACATATTAGCTACCATTGCTCAAGCTGATTCTAATGTGGATTCAAGTTTGAGTTCAGGCCTCTCATCTCCATCCCCTGTTGTTTGCAGTCATTTGGATGATCTATATCATCTGCTCCAGAACAAAATTGGCAACGCTGATGATTTCTTTCCCCTGATTCTTCAAAACTGCCTGGGTACAAATGGGAGTTGTCCACTGTCTATCTTCAATTATGCTTTGCTGATTAAGTGTAATCTGAAAATGACTCAAGTTTCTAGACAAAATGGGGTCAGAAACTCAAAGAAAGTTGCTCGAAAAAGTTCTCGGCAGCTGTTTGTTCAAAAATTTTCCTGCAAAGCTCCAGTTTATCATAATTGCAGGATCTATGCAGATGATGGGCGGTTGCTTTGTTACTGTGACCGCAGGAAGCTTGAATGGTATCTTCGTCGGGATTTGGCGAAACTTGTTGATGAAAATCCACCTGCCATTATGCTTCTTTTTGAGCCCAAAGGCCGTCCTGAAGATGAAGGCAATGATTTTTACATTCAAAGTAAGAAAAATATAATGCACTTCCCGGAACATATGAAAAGCCACCGCTCTCATGATATTGTCCTAGTATGCGTGGACTGTCACGAAATAGCTCATGCCTCTGCTGAGAAGTACAAAAAACAGATCGCTGCAGAATATGGGATTCCACTTTTTGTTCGTAAAGTGGTTGTTTCAGAACAAGCTCATGCTATATCTGAGTCATCTGTGCCGGCAACATCTTTTGAGGAGGAAGGTGTATCTCCTTTACAATTGCGAACTGCTGCTATGGCTCTTTTGCGCCATGGACCAAGAATGCCATCCACGCGCCGTGACGTACTGACAGAGATTGTTATGAGATATTATGGAGGGAGGCAAATATCTGAGGAAGATTTAGAGAGTGCTTTACTGGTTGGCATGAGTCCTCACGAGAGAAGACGATTTGAAAAGAAGAAAGGGCTTTCTTTCGGAAAGTCTAGAAGGCGTGTTCTTTCAGATGCAGAGCAGGAGAAAAATACTGTTACCATGTCTGCATGTGCCAGTGTTAAAGCGTCAGAAGTTGACACTCTACATGAATCATGTACCAGTACAGCAGAAGCATTTATCATGGTGAAGGATGCTGCTGGTTTAGGCAACTCCTCTTTAAATTCATATTTGGGGTCTGATGACAAAGCTTCTGACGTTGTTCAGAACATGGACTCAGATATAAGTGGAGTCTCTGATATGCAGTTTGTATCTGTTGTAAATGCTGATGGTGATTGTGAGAGTAGTGCACAAAATGGATCAGTTGACGTCTACTATGCCAGAGATGATGCAATTTCCCAACCTAAACATAGATCAAAAATGTCTCTCTTAGGACATGGTCCACATGGTAAGCAAGTTGTGGAGCATCTGCTAAAGGAATATGGCGAAGATGGTATACGTCAGTTTTGTCAAAGGTGGAGACAAGTATTTGTTGAAGCTGTTCATCCTCGTTTCTTACCTTCCGGCTGGGATATTACGCACAGTGGTAGGAGGGACTTTGGCGAATTTAGTGTTTACAATCCTACCAAGAAGGCTTCTGCTGCCGCCGAGGGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0000178 GO:0000460 GO:0000785 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006403 GO:0006725 GO:0006807 GO:0007549 GO:0008150 GO:0008152 GO:0008298 GO:0008334 GO:0008408 GO:0009048 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010639 GO:0016070 GO:0016071 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032210 GO:0032211 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035327 GO:0040029 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045934 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051179 GO:0051641 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071025 GO:0071027 GO:0071028 GO:0071029 GO:0071030 GO:0071033 GO:0071034 GO:0071035 GO:0071043 GO:0071044 GO:0071046 GO:0071048 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902494 GO:1904356 GO:1904357 GO:1904872 GO:1905354 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1 HRDC

Protein Analysis

924

Amino Acids

103.96

Weight (kDa)

7.31

Isoelectric Point (pI)

53.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1654
AatII GACGTC 1 cut(s) 2484
Acc16I TGCGCA 1 cut(s) 1081
Acc36I ACCTGC 1 cut(s) 1654
AccBSI CCGCTC 1 cut(s) 1761
AccI GTMKAC 2 cut(s) 2484, 2608
AccII CGCG 2 cut(s) 756, 2017
AciI CCGC 8 cut(s) 159, 266, 563, 1009, 1564, 1585, 1759, 2757
AclWI GGATC 3 cut(s) 447, 1553, 2479
AcoI YGGCCR 1 cut(s) 551
AcsI RAATTY 6 cut(s) 247, 896, 1032, 1508, 2335, 2717
AcuI CTGAAG 3 cut(s) 44, 290, 1704
AcyI GRCGYC 2 cut(s) 94, 2481
AfaI GTAC 8 cut(s) 356, 1063, 1107, 1363, 1826, 2028, 2272, 2278
AfiI CCNNNNNNNGG 2 cut(s) 120, 158
AflII CTTAAG 1 cut(s) 448
AhlI ACTAGT 1 cut(s) 31
AjnI CCWGG 3 cut(s) 976, 1056, 1356
AjuI GAANNNNNNNTTGG 2 cut(s) 881, 913
Alw21I GWGCWC 1 cut(s) 2464
Alw26I GTCTC 4 cut(s) 362, 2410, 2541, 2626
Alw44I GTGCAC 1 cut(s) 2460
AlwI GGATC 3 cut(s) 447, 1553, 2479
Ama87I CYCGRG 2 cut(s) 77, 911
AoxI GGCC 3 cut(s) 551, 1243, 1675
ApaLI GTGCAC 1 cut(s) 2460
ApeKI GCWGC 7 cut(s) 439, 989, 1492, 1841, 1973, 2309, 2753
ApoI RAATTY 6 cut(s) 247, 896, 1032, 1508, 2335, 2717
ArsI GACNNNNNNTTYG 2 cut(s) 232, 264
Asp700I GAANNNNTTC 2 cut(s) 1483, 2652
AspLEI GCGC 4 cut(s) 756, 1082, 1992, 2019
AspS9I GGNCC 2 cut(s) 1997, 2552
AsuC2I CCSGG 1 cut(s) 1739
AsuHPI GGTGA 3 cut(s) 331, 2311, 2456
AvaI CYCGRG 2 cut(s) 77, 911
AvaII GGWCC 2 cut(s) 1997, 2552
BaeGI GKGCMC 2 cut(s) 113, 2464
BanII GRGCYC 1 cut(s) 1671
BarI GAAGNNNNNNTAC 2 cut(s) 2592, 2624
BauI CACGAG 2 cut(s) 1101, 2117
BbsI GAAGAC 4 cut(s) 61, 97, 765, 2131
Bbv12I GWGCWC 1 cut(s) 2464
BbvI GCAGC 7 cut(s) 451, 976, 1504, 1828, 1960, 2296, 2740
BccI CCATC 6 cut(s) 277, 1264, 1553, 2017, 2435, 2597
BceAI ACGGC 2 cut(s) 1662, 2004
BcgI CGANNNNNNTGC 2 cut(s) 785, 819
BciT130I CCWGG 3 cut(s) 978, 1058, 1358
BcnI CCSGG 1 cut(s) 1739
BcoDI GTCTC 4 cut(s) 362, 2410, 2541, 2626
BcuI ACTAGT 1 cut(s) 31
BfaI CTAG 6 cut(s) 32, 570, 918, 1440, 1778, 2166
BfmI CTRYAG 2 cut(s) 1182, 1842
BfrI CTTAAG 1 cut(s) 448
BfuAI ACCTGC 1 cut(s) 1654
BisI GCNGC 9 cut(s) 440, 990, 1009, 1493, 1842, 1974, 2310, 2754, 2757
BlpI GCTNAGC 1 cut(s) 852
BlsI GCNGC 9 cut(s) 441, 991, 1010, 1494, 1843, 1975, 2311, 2755, 2758
Bme1390I CCNGG 4 cut(s) 978, 1058, 1358, 1739
Bme18I GGWCC 2 cut(s) 1997, 2552
BmeT110I CYCGRG 2 cut(s) 77, 911
BmgT120I GGNCC 2 cut(s) 1997, 2552
BmrFI CCNGG 4 cut(s) 978, 1058, 1358, 1739
BmsI GCATC 8 cut(s) 262, 556, 802, 1001, 2176, 2296, 2491, 2587
BoxI GACNNNNGTC 1 cut(s) 778
BpiI GAAGAC 4 cut(s) 61, 97, 765, 2131
BpmI CTGGAG 4 cut(s) 784, 999, 1286, 1509
Bpu1102I GCTNAGC 1 cut(s) 852
BpuEI CTTGAG 5 cut(s) 213, 320, 1141, 1191, 1416
BpuMI CCSGG 1 cut(s) 1739
BsaBI GATNNNNATC 2 cut(s) 1290, 1638
BsaHI GRCGYC 2 cut(s) 94, 2481
BsaJI CCNNGG 7 cut(s) 76, 293, 589, 1057, 1357, 1993, 2760
BsaXI ACNNNNNCTCC 2 cut(s) 178, 208
Bsc4I CCNNNNNNNGG 2 cut(s) 120, 158
Bse118I RCCGGY 1 cut(s) 1921
Bse1I ACTGG 8 cut(s) 554, 705, 767, 870, 1526, 2103, 2229, 2274
Bse3DI GCAATG 2 cut(s) 1200, 1702
Bse8I GATNNNNATC 2 cut(s) 1290, 1638
BseBI CCWGG 3 cut(s) 978, 1058, 1358
BseDI CCNNGG 7 cut(s) 76, 293, 589, 1057, 1357, 1993, 2760
BseGI GGATG 7 cut(s) 253, 571, 1256, 1288, 2009, 2311, 2656
BseJI GATNNNNATC 2 cut(s) 1290, 1638
BseLI CCNNNNNNNGG 2 cut(s) 120, 158
BseMI GCAATG 2 cut(s) 1200, 1702
BseMII CTCAG 8 cut(s) 363, 426, 483, 843, 1809, 1899, 2064, 2403
BseNI ACTGG 8 cut(s) 554, 705, 767, 870, 1526, 2103, 2229, 2274
BseRI GAGGAG 3 cut(s) 206, 1952, 2317
BseSI GKGCMC 2 cut(s) 113, 2464
BseXI GCAGC 7 cut(s) 451, 976, 1504, 1828, 1960, 2296, 2740
BseYI CCCAGC 1 cut(s) 2679
BsgI GTGCAG 1 cut(s) 500
Bsh1236I CGCG 2 cut(s) 756, 2017
BshFI GGCC 3 cut(s) 553, 1245, 1677
BsiHKAI GWGCWC 1 cut(s) 2464
BsiHKCI CYCGRG 2 cut(s) 77, 911
BsiSI CCGG 3 cut(s) 1739, 1922, 2676
BsiWI CGTACG 1 cut(s) 354
BslFI GGGAC 1 cut(s) 2720
BslI CCNNNNNNNGG 2 cut(s) 120, 158
BsmAI GTCTC 4 cut(s) 362, 2410, 2541, 2626
BsmFI GGGAC 1 cut(s) 2720
BsmI GAATGC 1 cut(s) 2010
BsnI GGCC 3 cut(s) 553, 1245, 1677
BsoBI CYCGRG 2 cut(s) 77, 911
Bsp1286I GDGCHC 3 cut(s) 113, 1671, 2464
Bsp1407I TGTACA 1 cut(s) 1105
Bsp143I GATC 7 cut(s) 452, 1138, 1285, 1545, 1836, 2471, 2525
Bsp1720I GCTNAGC 1 cut(s) 852
Bsp19I CCATGG 2 cut(s) 589, 1993
BspACI CCGC 8 cut(s) 159, 266, 563, 1009, 1564, 1585, 1759, 2757
BspANI GGCC 3 cut(s) 553, 1245, 1677
BspCNI CTCAG 8 cut(s) 364, 427, 484, 844, 1810, 1900, 2065, 2402
BspFNI CGCG 2 cut(s) 756, 2017
BspHI TCATGA 1 cut(s) 1765
BspMAI CTGCAG 1 cut(s) 1846
BspMI ACCTGC 1 cut(s) 1654
BspPI GGATC 3 cut(s) 447, 1553, 2479
BspTI CTTAAG 1 cut(s) 448
BsrBI CCGCTC 1 cut(s) 1761
BsrDI GCAATG 2 cut(s) 1200, 1702
BsrFI RCCGGY 1 cut(s) 1921
BsrGI TGTACA 1 cut(s) 1105
BsrI ACTGG 8 cut(s) 554, 705, 767, 870, 1526, 2103, 2229, 2274
BssAI RCCGGY 1 cut(s) 1921
BssECI CCNNGG 7 cut(s) 76, 293, 589, 1057, 1357, 1993, 2760
BssMI GATC 7 cut(s) 452, 1138, 1285, 1545, 1836, 2471, 2525
BssNAI GTATAC 1 cut(s) 2609
BssNI GRCGYC 2 cut(s) 94, 2481
BssSI CACGAG 2 cut(s) 1101, 2117
BssT1I CCWWGG 2 cut(s) 589, 1993
Bst1107I GTATAC 1 cut(s) 2609
Bst2BI CACGAG 2 cut(s) 1101, 2117
Bst2UI CCWGG 3 cut(s) 978, 1058, 1358
Bst4CI ACNGT 9 cut(s) 156, 319, 725, 1066, 1383, 1580, 1793, 2209, 2696
Bst6I CTCTTC 2 cut(s) 656, 1021
BstACI GRCGYC 2 cut(s) 94, 2481
BstAFI CTTAAG 1 cut(s) 448
BstAPI GCANNNNNTGC 1 cut(s) 1077
BstAUI TGTACA 1 cut(s) 1105
BstC8I GCNNGC 2 cut(s) 670, 1923
BstDEI CTNAG 9 cut(s) 372, 435, 492, 852, 1818, 1908, 2073, 2389, 2542
BstDSI CCRYGG 2 cut(s) 589, 1993
BstF5I GGATG 7 cut(s) 253, 571, 1256, 1288, 2009, 2311, 2656
BstFNI CGCG 2 cut(s) 756, 2017
BstHHI GCGC 4 cut(s) 756, 1082, 1992, 2019
BstKTI GATC 7 cut(s) 455, 1141, 1288, 1548, 1839, 2474, 2528
BstMAI GTCTC 4 cut(s) 362, 2410, 2541, 2626
BstMBI GATC 7 cut(s) 452, 1138, 1285, 1545, 1836, 2471, 2525
BstMWI GCNNNNNNNGC 7 cut(s) 1077, 1151, 1166, 1202, 1979, 2014, 2753
BstNI CCWGG 3 cut(s) 978, 1058, 1358
BstNSI RCATGY 1 cut(s) 2226
BstPAI GACNNNNGTC 1 cut(s) 778
BstSCI CCNGG 4 cut(s) 976, 1056, 1356, 1737
BstSFI CTRYAG 2 cut(s) 1182, 1842
BstSLI GKGCMC 2 cut(s) 113, 2464
BstUI CGCG 2 cut(s) 756, 2017
BstV1I GCAGC 7 cut(s) 451, 976, 1504, 1828, 1960, 2296, 2740
BstV2I GAAGAC 4 cut(s) 61, 97, 765, 2131
BstX2I RGATCY 2 cut(s) 452, 1545
BstYI RGATCY 2 cut(s) 452, 1545
BstZ17I GTATAC 1 cut(s) 2609
BsuRI GGCC 3 cut(s) 553, 1245, 1677
BtgI CCRYGG 2 cut(s) 589, 1993
BtsCI GGATG 7 cut(s) 253, 571, 1256, 1288, 2009, 2311, 2656
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 7 cut(s) 161, 273, 314, 698, 1379, 2236, 2701
BveI ACCTGC 1 cut(s) 1654
Cac8I GCNNGC 2 cut(s) 670, 1923
CciI TCATGA 1 cut(s) 1765
CfoI GCGC 4 cut(s) 756, 1082, 1992, 2019
Cfr10I RCCGGY 1 cut(s) 1921
Cfr13I GGNCC 2 cut(s) 1997, 2552
CseI GACGC 2 cut(s) 102, 2229
Csp6I GTAC 8 cut(s) 355, 1062, 1106, 1362, 1825, 2027, 2271, 2277
CviQI GTAC 8 cut(s) 355, 1062, 1106, 1362, 1825, 2027, 2271, 2277
DdeI CTNAG 9 cut(s) 372, 435, 492, 852, 1818, 1908, 2073, 2389, 2542
DpnI GATC 7 cut(s) 454, 1140, 1287, 1547, 1838, 2473, 2527
DpnII GATC 7 cut(s) 452, 1138, 1285, 1545, 1836, 2471, 2525
DraI TTTAAA 2 cut(s) 210, 2334
EaeI YGGCCR 1 cut(s) 551
Eam1104I CTCTTC 2 cut(s) 656, 1021
EarI CTCTTC 2 cut(s) 656, 1021
EciI GGCGGA 1 cut(s) 281
Eco130I CCWWGG 2 cut(s) 589, 1993
Eco147I AGGCCT 1 cut(s) 1245
Eco24I GRGCYC 1 cut(s) 1671
Eco47I GGWCC 2 cut(s) 1997, 2552
Eco57I CTGAAG 3 cut(s) 44, 290, 1704
Eco88I CYCGRG 2 cut(s) 77, 911
EcoRI GAATTC 1 cut(s) 247
EcoRII CCWGG 3 cut(s) 976, 1056, 1356
EcoT14I CCWWGG 2 cut(s) 589, 1993
EcoT38I GRGCYC 1 cut(s) 1671
ErhI CCWWGG 2 cut(s) 589, 1993
FalI AAGNNNNNCTT 2 cut(s) 158, 190
FaqI GGGAC 1 cut(s) 2720
FauNDI CATATG 1 cut(s) 1746
FblI GTMKAC 2 cut(s) 2484, 2608
Fnu4HI GCNGC 9 cut(s) 440, 990, 1009, 1493, 1842, 1974, 2310, 2754, 2757
FokI GGATG 7 cut(s) 240, 578, 1243, 1295, 1996, 2318, 2643
FriOI GRGCYC 1 cut(s) 1671
Fsp4HI GCNGC 9 cut(s) 440, 990, 1009, 1493, 1842, 1974, 2310, 2754, 2757
FspAI RTGCGCAY 1 cut(s) 1081
FspBI CTAG 6 cut(s) 32, 570, 918, 1440, 1778, 2166
FspI TGCGCA 1 cut(s) 1081
GlaI GCGC 4 cut(s) 755, 1081, 1991, 2018
GluI GCNGC 9 cut(s) 440, 990, 1009, 1493, 1842, 1974, 2310, 2754, 2757
GsaI CCCAGC 1 cut(s) 2683
GsuI CTGGAG 4 cut(s) 784, 999, 1286, 1509
HaeIII GGCC 3 cut(s) 553, 1245, 1677
HapII CCGG 3 cut(s) 1739, 1922, 2676
HgaI GACGC 2 cut(s) 102, 2229
HhaI GCGC 4 cut(s) 756, 1082, 1992, 2019
Hin1I GRCGYC 2 cut(s) 94, 2481
Hin6I GCGC 4 cut(s) 754, 1080, 1990, 2017
HinP1I GCGC 4 cut(s) 754, 1080, 1990, 2017
HincII GTYRAC 3 cut(s) 783, 2251, 2479
HindII GTYRAC 3 cut(s) 783, 2251, 2479
HindIII AAGCTT 3 cut(s) 969, 1592, 2361
HpaII CCGG 3 cut(s) 1739, 1922, 2676
HphI GGTGA 3 cut(s) 331, 2311, 2456
Hpy99I CGWCG 1 cut(s) 1614
HpyAV CCTTC 9 cut(s) 379, 409, 554, 1685, 1937, 2163, 2296, 2682, 2737
HpyCH4III ACNGT 9 cut(s) 156, 319, 725, 1066, 1383, 1580, 1793, 2209, 2696
HpyCH4IV ACGT 5 cut(s) 357, 2025, 2370, 2481, 2611
HpyF10VI GCNNNNNNNGC 7 cut(s) 1077, 1151, 1166, 1202, 1979, 2014, 2753
HpyF3I CTNAG 9 cut(s) 372, 435, 492, 852, 1818, 1908, 2073, 2389, 2542
HpySE526I ACGT 5 cut(s) 357, 2025, 2370, 2481, 2611
Hsp92I GRCGYC 2 cut(s) 94, 2481
HspAI GCGC 4 cut(s) 754, 1080, 1990, 2017
KroI GCCGGC 1 cut(s) 1921
KroNI GCCGGC 1 cut(s) 1923
Kzo9I GATC 7 cut(s) 452, 1138, 1285, 1545, 1836, 2471, 2525
LmnI GCTCC 4 cut(s) 1174, 1305, 1528, 2575
Lsp1109I GCAGC 7 cut(s) 451, 976, 1504, 1828, 1960, 2296, 2740
LweI GCATC 8 cut(s) 262, 556, 802, 1001, 2176, 2296, 2491, 2587
MaeI CTAG 6 cut(s) 32, 570, 918, 1440, 1778, 2166
MaeII ACGT 5 cut(s) 357, 2025, 2370, 2481, 2611
MaeIII GTNAC 8 cut(s) 271, 733, 747, 1574, 1580, 1793, 2021, 2209
MalI GATC 7 cut(s) 454, 1140, 1287, 1547, 1838, 2473, 2527
MbiI CCGCTC 1 cut(s) 1761
MboI GATC 7 cut(s) 452, 1138, 1285, 1545, 1836, 2471, 2525
MfeI CAATTG 1 cut(s) 1961
MflI RGATCY 2 cut(s) 452, 1545
MhlI GDGCHC 3 cut(s) 113, 1671, 2464
MlyI GAGTC 8 cut(s) 359, 383, 992, 1423, 1919, 2119, 2381, 2412
MmeI TCCRAC 1 cut(s) 1196
MroNI GCCGGC 1 cut(s) 1921
MroXI GAANNNNTTC 2 cut(s) 1483, 2652
MseI TTAA 9 cut(s) 209, 404, 449, 645, 845, 1094, 1410, 2235, 2333
MslI CAYNNNNRTG 3 cut(s) 317, 636, 1281
MspA1I CMGCKG 2 cut(s) 22, 1495
MspCI CTTAAG 1 cut(s) 448
MspI CCGG 3 cut(s) 1739, 1922, 2676
MspR9I CCNGG 4 cut(s) 978, 1058, 1358, 1739
MunI CAATTG 1 cut(s) 1961
Mva1269I GAATGC 1 cut(s) 2010
MvaI CCWGG 3 cut(s) 978, 1058, 1358
MvnI CGCG 2 cut(s) 756, 2017
MwoI GCNNNNNNNGC 7 cut(s) 1077, 1151, 1166, 1202, 1979, 2014, 2753
NaeI GCCGGC 1 cut(s) 1923
NciI CCSGG 1 cut(s) 1739
NcoI CCATGG 2 cut(s) 589, 1993
NdeI CATATG 1 cut(s) 1746
NdeII GATC 7 cut(s) 452, 1138, 1285, 1545, 1836, 2471, 2525
NgoMIV GCCGGC 1 cut(s) 1921
NmeAIII GCCGAG 2 cut(s) 274, 1468
NmuCI GTSAC 5 cut(s) 271, 733, 1580, 1793, 2021
NsbI TGCGCA 1 cut(s) 1081
NspI RCATGY 1 cut(s) 2226
PaeR7I CTCGAG 1 cut(s) 911
PagI TCATGA 1 cut(s) 1765
PaqCI CACCTGC 1 cut(s) 1654
PceI AGGCCT 1 cut(s) 1245
PcsI WCGNNNNNNNCGW 1 cut(s) 94
PctI GAATGC 1 cut(s) 2010
PdiI GCCGGC 1 cut(s) 1923
PdmI GAANNNNTTC 2 cut(s) 1483, 2652
PfeI GAWTC 7 cut(s) 162, 716, 1214, 1226, 1342, 1855, 2264
Pfl23II CGTACG 1 cut(s) 354
PflFI GACNNNGTC 2 cut(s) 373, 2550
PfoI TCCNGGA 2 cut(s) 976, 1737
PkrI GCNGC 9 cut(s) 441, 991, 1010, 1494, 1843, 1975, 2311, 2755, 2758
PleI GAGTC 8 cut(s) 358, 382, 991, 1423, 1918, 2118, 2381, 2411
PpsI GAGTC 8 cut(s) 358, 382, 991, 1423, 1918, 2118, 2381, 2411
PshAI GACNNNNGTC 1 cut(s) 778
Psp6I CCWGG 3 cut(s) 976, 1056, 1356
PspFI CCCAGC 1 cut(s) 2679
PspGI CCWGG 3 cut(s) 976, 1056, 1356
PspLI CGTACG 1 cut(s) 354
PspPI GGNCC 2 cut(s) 1997, 2552
PstI CTGCAG 1 cut(s) 1846
PsuI RGATCY 2 cut(s) 452, 1545
PsyI GACNNNGTC 2 cut(s) 373, 2550
PvuII CAGCTG 2 cut(s) 22, 1495
RsaI GTAC 8 cut(s) 356, 1063, 1107, 1363, 1826, 2028, 2272, 2278
RsaNI GTAC 8 cut(s) 355, 1062, 1106, 1362, 1825, 2027, 2271, 2277
RseI CAYNNNNRTG 3 cut(s) 317, 636, 1281
SaqAI TTAA 9 cut(s) 209, 404, 449, 645, 845, 1094, 1410, 2235, 2333
SatI GCNGC 9 cut(s) 440, 990, 1009, 1493, 1842, 1974, 2310, 2754, 2757
Sau3AI GATC 7 cut(s) 452, 1138, 1285, 1545, 1836, 2471, 2525
Sau96I GGNCC 2 cut(s) 1997, 2552
SchI GAGTC 8 cut(s) 359, 383, 992, 1423, 1919, 2119, 2381, 2412
ScrFI CCNGG 4 cut(s) 978, 1058, 1358, 1739
SduI GDGCHC 3 cut(s) 113, 1671, 2464
SfaNI GCATC 8 cut(s) 262, 556, 802, 1001, 2176, 2296, 2491, 2587
SfcI CTRYAG 2 cut(s) 1182, 1842
Sfr274I CTCGAG 1 cut(s) 911
SinI GGWCC 2 cut(s) 1997, 2552
SlaI CTCGAG 1 cut(s) 911
SmiMI CAYNNNNRTG 3 cut(s) 317, 636, 1281
SmlI CTYRAG 7 cut(s) 228, 299, 448, 911, 1120, 1206, 1431
SmoI CTYRAG 7 cut(s) 228, 299, 448, 911, 1120, 1206, 1431
SpeI ACTAGT 1 cut(s) 31
SseBI AGGCCT 1 cut(s) 1245
SsiI CCGC 8 cut(s) 159, 266, 563, 1009, 1564, 1585, 1759, 2757
SspMI CTAG 6 cut(s) 32, 570, 918, 1440, 1778, 2166
StuI AGGCCT 1 cut(s) 1245
StyD4I CCNGG 4 cut(s) 976, 1056, 1356, 1737
StyI CCWWGG 2 cut(s) 589, 1993
TaaI ACNGT 9 cut(s) 156, 319, 725, 1066, 1383, 1580, 1793, 2209, 2696
TaiI ACGT 5 cut(s) 360, 2028, 2373, 2484, 2614
TaqI TCGA 4 cut(s) 654, 805, 912, 1477
TatI WGTACW 3 cut(s) 1105, 1824, 2276
TauI GCSGC 2 cut(s) 1011, 2759
TfiI GAWTC 7 cut(s) 162, 716, 1214, 1226, 1342, 1855, 2264
Tru1I TTAA 9 cut(s) 209, 404, 449, 645, 845, 1094, 1410, 2235, 2333
Tru9I TTAA 9 cut(s) 209, 404, 449, 645, 845, 1094, 1410, 2235, 2333
TscAI CASTG 7 cut(s) 161, 280, 314, 705, 1386, 2236, 2701
TseFI GTSAC 5 cut(s) 271, 733, 1580, 1793, 2021
TseI GCWGC 7 cut(s) 439, 989, 1492, 1841, 1973, 2309, 2753
Tsp45I GTSAC 5 cut(s) 271, 733, 1580, 1793, 2021
TspDTI ATGAA 8 cut(s) 888, 1026, 1650, 1704, 1763, 2277, 2328, 2645
TspGWI ACGGA 2 cut(s) 186, 1035
TspRI CASTG 7 cut(s) 161, 280, 314, 705, 1386, 2236, 2701
Tth111I GACNNNGTC 2 cut(s) 373, 2550
Vha464I CTTAAG 1 cut(s) 448
VneI GTGCAC 1 cut(s) 2460
VpaK11BI GGWCC 2 cut(s) 1997, 2552
XapI RAATTY 6 cut(s) 247, 896, 1032, 1508, 2335, 2717
XbaI TCTAGA 2 cut(s) 1439, 2165
XceI RCATGY 1 cut(s) 2226
XcmI CCANNNNNNNNNTGG 1 cut(s) 1310
XhoI CTCGAG 1 cut(s) 911
XmiI GTMKAC 2 cut(s) 2484, 2608
XmnI GAANNNNTTC 2 cut(s) 1483, 2652
XspI CTAG 6 cut(s) 32, 570, 918, 1440, 1778, 2166
ZraI GACGTC 1 cut(s) 2482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.