pycom09g03190

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0005671
Physical Location & Seq
Reverse (-)
2346548 .. 2352290
5743 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g03190.5

Sequence Viewer

Length: 2982 bp
ATGTGCCACGTAAGCCCCCCTCTCCCACCGTTCCGCCGCAAACCGCAATCGTCTTCCTTCTTCTGCTTCCATCGAGCTACAGAAAGCTCCAGAAACACAAAAATGGAGGCGAAAGGAAGAAACCTCAAGATTGCAGTAGTCGCCCTCGCTTCTCTGACAGCTCTCTCCGTCTTCTTCTTTGCGCGGCACCGGAGACGCCGCCGGAACGCAAACTGCCGCCTAAGTCGATGTTATTTACGAGCTGATCACCTCAAGCCGCAGTACGGATTCAAGCGAGTTTTAGCTGACAATTCCTACTCTCCCTTCAAGCATCTGAACCTGAATGGTTCTCCGGAAGATAAGAACTGGAATTCGCATCCTTATGAGGCGGAAATCACGGGATTGATGGAAAGTTTTCAGCTTGAGGAGTTTCGAAAATGCATTGGAATTACAGAGAAGAATGTGGGGGGTTTGGAAGAAATGAGTGAGTCGTATGAGTGGGTGGAGACTGAGTCGAAGTTGAAGGAACTCGTTGAGGTTTTGAGCAAAGAGAAGGTGTTTGCTGTTGATACCGAGCAGCATAGTTTTCGGTCTTTTCTCGGTTTTACAGCTCTAATACAGATTTCTACTGAGAAGGAAGATTACTTGGTGGACACGATTGCTCTGCATGATTGCATAAGCCTTCTACGGCCGGTTTTCGCTGATGCTAGTATTTGTAAGGTGTTCCATGGGGCGGATAGTGATGTCCTCTGGCTGCAAAGGGACTTCCATATTTATGTTGTTAATATGTTTGATACTGCAAAGGCTTGCGAAGTTTTGTCAAAGCCACAAAAGTCACTGGCATATTTACTTGAATCTTACTGTGGAGTAGTCACTAACAAATTGTTACAGCGTGAAGACTGGAGACAGCGCCCCTTGTCAATCGAAATGGTACAATATGCTCGTACAGATGCACACTATCTACTCTATATTGCACACAGTTTAATTGCTGAGCTGGAAATGCTGGATAACGAAAACACGGGTCCCAATGACAAATTACATTTTGTTCTCGAGGCTAGTCGGCGTTCAAACTCGATATGTTTGCAACTGTATACTAAAGAGATAGAAGCTTCTCCTGGAGAGTCTGCTGCTTCATCAATTTTTAGCCGTCATTTGAACGGCCAAGGAGGGATTTCATCAGTTTCTCCTGAAGTCCAGGATGCTGTGAACAGATTATGTGCATGGAGAGATTTGATGGCTCGTGTACATGATGAGAGCTTAAGATATGTAATATCAGATCAGGCTATTGTTGCCCTTGCAGACAAAGCTCCAACGAACCATGCAGACAAAGCTCCAACAAACCCTGCAGACATATTAACCACCATTGCTGAAGCTGATTCCAATGCAGATTCAAGTTTCAGTTCTGGCCTCTCATCTCCATCCCCTGTCGTTTACAGTCATTTGGATGACTTTCGTCATCTGCTCCACAACAAAATAGGCAACCCTGATAATTTCTTTTCTAAGATTCTTCAAAACTGCCTGGGTCCAAATGGGAGTTGCCCACTGTCCATCTTCAATTATGCTTTACTGGTAGAGTGTAATATGAAAGTGCCACTAACTTCTAGAAAAAATGGGTTGAGAAATTCAAAGCAAGTTGCCCGAAAGTCGTCTCGGCAGCTATTTGTTCAAAAATTTTCCTGTAAAGCTCCAGTTTATCATAATTGCAGGATCTATGCAGATGATGGCCGGTTGCTTTGTTACTGTGACCGCAGGAAGCTTGAATGGTATCTTCGGCGGGATTTGGCAAAACTTCTTGATGAAAATCCACCTGCGATTATGCTTCTTTTTGAGCCCAAAGGCCGTCCAGAAGATGAAGGCAATGATTTTTACATTCAAAGTAAGAAAAATATATGTGTTAGCTGTGGCGAAAGAAATCATTACTTACGCTACCGAATAATTCCCTCATGCTACAGAATGCACTTCCCTGAACATTTGAAAAGCCACCGTTCTCATGATATTGTCCTACTATGTGTGGATTGTCATGAAATTGCTCATGCCTCTGCTGAGAAGTATAAAAAACATATCGCTGCAGAATTTGGGATTCCACTTTTTGTTCGCAGAGTGGTTGATTCAGAACAATCTCAGGCTACATCTGAATCATCTGTTCCAGAAACAACTTTTGAGGAGCCAGGTGTATCTCCTTTACAATTGCGCATTGCTGCTATGGCTCTTCTGCGCCATGGACCAAGAATGCCATCCAGCCGCCGTGAAGAACTGACAGAGATAGTAATGAGGTATTATGGAGGGAGGCAAATATCTGAGGAAGATTTAGAAAACGCTTTGTTGGTTGGCATGAGTCCCAACGAGAGAAAACGATTTGAGAAGAAGAAAGGACTTTCTTTAAAGCATTCTAAGGGGATTATTCAACCAGATGCAGAGCAAGAGAGAACTACAATTACCATGTACACATCTGCCATTGTGAACGCATCAGAAGCTGATACTCTACTTGATTCATGCACCACTGAAGCAGAAGCATATACCGGAACAGAAAATGGAGATGCTTTCATGCTGAAGGATGCTGATTTGGGGACTGAAGAAGAAGCTTTTGCTGTTGTTGAAAAGAACATGAACTCAGATATAAGTGGAGTTGCTGGTAGTCAGTTTATATCTGTTGTAAATGCTGATGGTGATGGTGACGGTGATGTTGAGAGAGATACACCGAACGGATCAGTTGATTTTAGTCATGCCGGAGATGGTGCAACTTCCCACCGTAAACGTAGATCAAAAATGTCACTTTTAGGACATGGTCCACATGGCAAGCAAGTTGTGGATCATCTGCTAAAGGAATATGGTGAAGATGGCATTTCTCAATTTTGTCAAAGGTGGAGACAAGTATTCGTTGAATCTCTTCATCCTCGTTTTTTACCTGCCGGCTGGGATATAACGCACAGTGGTAGGAGGGACTTCGGCGAATTTAGCGTCTACAATCCTCTCAAGAAGGCTTCTGCTGCTGAATGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0000178 GO:0000460 GO:0000785 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006403 GO:0006725 GO:0006807 GO:0007549 GO:0008150 GO:0008152 GO:0008298 GO:0008334 GO:0008408 GO:0009048 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010639 GO:0016070 GO:0016071 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032210 GO:0032211 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035327 GO:0040029 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045934 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051179 GO:0051641 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071025 GO:0071027 GO:0071028 GO:0071029 GO:0071030 GO:0071033 GO:0071034 GO:0071035 GO:0071043 GO:0071044 GO:0071046 GO:0071048 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902494 GO:1904356 GO:1904357 GO:1904872 GO:1905354 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1 HRDC

Protein Analysis

994

Amino Acids

112.1

Weight (kDa)

6.51

Isoelectric Point (pI)

54.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1795
Acc16I TGCGCA 1 cut(s) 2171
Acc36I ACCTGC 2 cut(s) 1795, 2893
AccB1I GGYRCC 1 cut(s) 186
AccI GTMKAC 2 cut(s) 1070, 2940
AccII CGCG 1 cut(s) 184
AccIII TCCGGA 1 cut(s) 331
AclWI GGATC 3 cut(s) 1694, 2692, 2796
AcoI YGGCCR 3 cut(s) 668, 1138, 1702
AcsI RAATTY 5 cut(s) 349, 1600, 1649, 2051, 2930
AcuI CTGAAG 5 cut(s) 1188, 1368, 2502, 2549, 2571
AcyI GRCGYC 1 cut(s) 196
AfaI GTAC 5 cut(s) 263, 912, 925, 1224, 2423
AfiI CCNNNNNNNGG 2 cut(s) 263, 712
AflII CTTAAG 1 cut(s) 1237
AjnI CCWGG 4 cut(s) 1093, 1173, 1497, 2146
Alw26I GTCTC 5 cut(s) 187, 479, 877, 1632, 2839
AlwI GGATC 3 cut(s) 1694, 2692, 2796
AlwNI CAGNNNCTG 1 cut(s) 2453
Ama87I CYCGRG 1 cut(s) 1028
Aor13HI TCCGGA 1 cut(s) 331
AoxI GGCC 5 cut(s) 668, 1138, 1384, 1702, 1816
ApeKI GCWGC 7 cut(s) 556, 733, 1106, 1633, 2045, 2177, 2966
ApoI RAATTY 5 cut(s) 349, 1600, 1649, 2051, 2930
ArsI GACNNNNNNTTYG 2 cut(s) 2922, 2954
Asp700I GAANNNNTTC 2 cut(s) 393, 2865
AspLEI GCGC 4 cut(s) 184, 891, 2172, 2196
AspS9I GGNCC 4 cut(s) 1001, 1502, 2201, 2765
AsuHPI GGTGA 5 cut(s) 239, 2657, 2663, 2669, 2822
AsuII TTCGAA 1 cut(s) 412
AvaI CYCGRG 1 cut(s) 1028
AvaII GGWCC 4 cut(s) 1001, 1502, 2201, 2765
BanI GGYRCC 1 cut(s) 186
BanII GRGCYC 1 cut(s) 1812
BarI GAAGNNNNNNTAC 2 cut(s) 605, 637
BauI CACGAG 1 cut(s) 1218
BbsI GAAGAC 3 cut(s) 45, 163, 882
BbvI GCAGC 7 cut(s) 568, 720, 1093, 1645, 2032, 2164, 2953
BceAI ACGGC 5 cut(s) 683, 1110, 1153, 1803, 2208
BcgI CGANNNNNNTGC 6 cut(s) 548, 582, 625, 659, 1042, 1076
BciT130I CCWGG 4 cut(s) 1095, 1175, 1499, 2148
BclI TGATCA 1 cut(s) 244
BcoDI GTCTC 5 cut(s) 187, 479, 877, 1632, 2839
BfaI CTAG 3 cut(s) 687, 1035, 1581
BfmI CTRYAG 4 cut(s) 78, 1323, 1927, 2046
BfoI RGCGCY 1 cut(s) 892
BfrI CTTAAG 1 cut(s) 1237
BfuAI ACCTGC 2 cut(s) 1795, 2893
BlpI GCTNAGC 1 cut(s) 969
Bme1390I CCNGG 4 cut(s) 1095, 1175, 1499, 2148
Bme18I GGWCC 4 cut(s) 1001, 1502, 2201, 2765
BmeT110I CYCGRG 1 cut(s) 1028
BmgT120I GGNCC 4 cut(s) 1001, 1502, 2201, 2765
BmiI GGNNCC 5 cut(s) 188, 1002, 1003, 1503, 2145
BmrFI CCNGG 4 cut(s) 1095, 1175, 1499, 2148
BmsI GCATC 9 cut(s) 319, 364, 673, 919, 1168, 2380, 2453, 2506, 2524
BoxI GACNNNNGTC 1 cut(s) 1431
BpiI GAAGAC 3 cut(s) 45, 163, 882
BpmI CTGGAG 4 cut(s) 73, 901, 1116, 1650
Bpu1102I GCTNAGC 1 cut(s) 969
Bpu14I TTCGAA 1 cut(s) 412
BpuEI CTTGAG 4 cut(s) 110, 236, 422, 2936
BsaAI YACGTR 1 cut(s) 10
BsaBI GATNNNNATC 1 cut(s) 1779
BsaHI GRCGYC 1 cut(s) 196
BsaJI CCNNGG 4 cut(s) 706, 1141, 1498, 2197
BsaWI WCCGGW 3 cut(s) 189, 331, 2498
BsaXI ACNNNNNCTCC 2 cut(s) 476, 506
Bsc4I CCNNNNNNNGG 2 cut(s) 263, 712
Bse118I RCCGGY 3 cut(s) 670, 1704, 2888
Bse1I ACTGG 5 cut(s) 350, 822, 884, 1551, 1667
Bse3DI GCAATG 3 cut(s) 1341, 1843, 2172
Bse8I GATNNNNATC 1 cut(s) 1779
BseAI TCCGGA 1 cut(s) 331
BseBI CCWGG 4 cut(s) 1095, 1175, 1499, 2148
BseDI CCNNGG 4 cut(s) 706, 1141, 1498, 2197
BseGI GGATG 7 cut(s) 355, 1183, 1397, 1429, 2213, 2539, 2869
BseJI GATNNNNATC 1 cut(s) 1779
BseLI CCNNNNNNNGG 2 cut(s) 263, 712
BseMI GCAATG 3 cut(s) 1341, 1843, 2172
BseMII CTCAG 7 cut(s) 480, 600, 960, 2013, 2114, 2268, 2604
BseNI ACTGG 5 cut(s) 350, 822, 884, 1551, 1667
BseRI GAGGAG 2 cut(s) 419, 2156
BseX3I CGGCCG 1 cut(s) 668
BseXI GCAGC 7 cut(s) 568, 720, 1093, 1645, 2032, 2164, 2953
BseYI CCCAGC 1 cut(s) 2892
Bsh1236I CGCG 1 cut(s) 184
Bsh1285I CGRYCG 1 cut(s) 671
BshFI GGCC 5 cut(s) 670, 1140, 1386, 1704, 1818
BshNI GGYRCC 1 cut(s) 186
BsiEI CGRYCG 1 cut(s) 671
BsiHKCI CYCGRG 1 cut(s) 1028
BsiSI CCGG 8 cut(s) 190, 202, 332, 671, 1705, 2499, 2706, 2889
BslFI GGGAC 5 cut(s) 755, 987, 2301, 2560, 2933
BslI CCNNNNNNNGG 2 cut(s) 263, 712
BsmAI GTCTC 5 cut(s) 187, 479, 877, 1632, 2839
BsmBI CGTCTC 2 cut(s) 187, 1632
BsmFI GGGAC 5 cut(s) 755, 987, 2301, 2560, 2933
BsmI GAATGC 3 cut(s) 1938, 2214, 2365
BsnI GGCC 5 cut(s) 670, 1140, 1386, 1704, 1818
BsoBI CYCGRG 1 cut(s) 1028
Bsp119I TTCGAA 1 cut(s) 412
Bsp1286I GDGCHC 1 cut(s) 1812
Bsp13I TCCGGA 1 cut(s) 331
Bsp1407I TGTACA 2 cut(s) 1222, 2421
Bsp143I GATC 6 cut(s) 244, 1255, 1686, 2684, 2738, 2788
Bsp1720I GCTNAGC 1 cut(s) 969
Bsp19I CCATGG 2 cut(s) 706, 2197
BspANI GGCC 5 cut(s) 670, 1140, 1386, 1704, 1818
BspCNI CTCAG 7 cut(s) 481, 601, 961, 2014, 2113, 2269, 2603
BspEI TCCGGA 1 cut(s) 331
BspFNI CGCG 1 cut(s) 184
BspHI TCATGA 2 cut(s) 1969, 1999
BspLI GGNNCC 5 cut(s) 188, 1002, 1003, 1503, 2145
BspMAI CTGCAG 2 cut(s) 1327, 2050
BspMI ACCTGC 2 cut(s) 1795, 2893
BspPI GGATC 3 cut(s) 1694, 2692, 2796
BspQI GCTCTTC 1 cut(s) 2193
BspT104I TTCGAA 1 cut(s) 412
BspT107I GGYRCC 1 cut(s) 186
BspTI CTTAAG 1 cut(s) 1237
BsrDI GCAATG 3 cut(s) 1341, 1843, 2172
BsrFI RCCGGY 3 cut(s) 670, 1704, 2888
BsrGI TGTACA 2 cut(s) 1222, 2421
BsrI ACTGG 5 cut(s) 350, 822, 884, 1551, 1667
BssAI RCCGGY 3 cut(s) 670, 1704, 2888
BssECI CCNNGG 4 cut(s) 706, 1141, 1498, 2197
BssMI GATC 6 cut(s) 244, 1255, 1686, 2684, 2738, 2788
BssNAI GTATAC 1 cut(s) 1071
BssNI GRCGYC 1 cut(s) 196
BssSI CACGAG 1 cut(s) 1218
BssT1I CCWWGG 3 cut(s) 706, 1141, 2197
Bst1107I GTATAC 1 cut(s) 1071
Bst2BI CACGAG 1 cut(s) 1218
Bst2UI CCWGG 4 cut(s) 1095, 1175, 1499, 2148
Bst6I CTCTTC 2 cut(s) 2193, 2871
BstACI GRCGYC 1 cut(s) 196
BstAFI CTTAAG 1 cut(s) 1237
BstAUI TGTACA 2 cut(s) 1222, 2421
BstBAI YACGTR 1 cut(s) 10
BstBI TTCGAA 1 cut(s) 412
BstC8I GCNNGC 3 cut(s) 787, 2777, 2890
BstDSI CCRYGG 2 cut(s) 706, 2197
BstF5I GGATG 7 cut(s) 355, 1183, 1397, 1429, 2213, 2539, 2869
BstFNI CGCG 1 cut(s) 184
BstH2I RGCGCY 1 cut(s) 892
BstHHI GCGC 4 cut(s) 184, 891, 2172, 2196
BstKTI GATC 6 cut(s) 247, 1258, 1689, 2687, 2741, 2791
BstMAI GTCTC 5 cut(s) 187, 479, 877, 1632, 2839
BstMBI GATC 6 cut(s) 244, 1255, 1686, 2684, 2738, 2788
BstMCI CGRYCG 1 cut(s) 671
BstNI CCWGG 4 cut(s) 1095, 1175, 1499, 2148
BstPAI GACNNNNGTC 1 cut(s) 1431
BstSCI CCNGG 4 cut(s) 1093, 1173, 1497, 2146
BstSFI CTRYAG 4 cut(s) 78, 1323, 1927, 2046
BstUI CGCG 1 cut(s) 184
BstV1I GCAGC 7 cut(s) 568, 720, 1093, 1645, 2032, 2164, 2953
BstV2I GAAGAC 3 cut(s) 45, 163, 882
BstX2I RGATCY 1 cut(s) 1686
BstYI RGATCY 1 cut(s) 1686
BstZ17I GTATAC 1 cut(s) 1071
BstZI CGGCCG 1 cut(s) 668
BsuRI GGCC 5 cut(s) 670, 1140, 1386, 1704, 1818
BtgI CCRYGG 2 cut(s) 706, 2197
BtsCI GGATG 7 cut(s) 355, 1183, 1397, 1429, 2213, 2539, 2869
BtsIMutI CAGTG 4 cut(s) 815, 1520, 2478, 2914
BveI ACCTGC 2 cut(s) 1795, 2893
Cac8I GCNNGC 3 cut(s) 787, 2777, 2890
CaiI CAGNNNCTG 1 cut(s) 2453
CciI TCATGA 2 cut(s) 1969, 1999
CfoI GCGC 4 cut(s) 184, 891, 2172, 2196
Cfr10I RCCGGY 3 cut(s) 670, 1704, 2888
Cfr13I GGNCC 4 cut(s) 1001, 1502, 2201, 2765
CseI GACGC 2 cut(s) 204, 2926
Csp6I GTAC 5 cut(s) 262, 911, 924, 1223, 2422
CviQI GTAC 5 cut(s) 262, 911, 924, 1223, 2422
DpnI GATC 6 cut(s) 246, 1257, 1688, 2686, 2740, 2790
DpnII GATC 6 cut(s) 244, 1255, 1686, 2684, 2738, 2788
DraI TTTAAA 1 cut(s) 2361
EaeI YGGCCR 3 cut(s) 668, 1138, 1702
EagI CGGCCG 1 cut(s) 668
Eam1104I CTCTTC 2 cut(s) 2193, 2871
EarI CTCTTC 2 cut(s) 2193, 2871
EciI GGCGGA 3 cut(s) 23, 383, 728
EclXI CGGCCG 1 cut(s) 668
Eco130I CCWWGG 3 cut(s) 706, 1141, 2197
Eco24I GRGCYC 1 cut(s) 1812
Eco47I GGWCC 4 cut(s) 1001, 1502, 2201, 2765
Eco52I CGGCCG 1 cut(s) 668
Eco57I CTGAAG 5 cut(s) 1188, 1368, 2502, 2549, 2571
Eco88I CYCGRG 1 cut(s) 1028
EcoO109I RGGNCCY 1 cut(s) 1001
EcoRI GAATTC 1 cut(s) 349
EcoRII CCWGG 4 cut(s) 1093, 1173, 1497, 2146
EcoT14I CCWWGG 3 cut(s) 706, 1141, 2197
EcoT22I ATGCAT 1 cut(s) 422
EcoT38I GRGCYC 1 cut(s) 1812
ErhI CCWWGG 3 cut(s) 706, 1141, 2197
Esp3I CGTCTC 2 cut(s) 187, 1632
FaqI GGGAC 5 cut(s) 755, 987, 2301, 2560, 2933
FauI CCCGC 1 cut(s) 1746
FbaI TGATCA 1 cut(s) 244
FblI GTMKAC 2 cut(s) 1070, 2940
FokI GGATG 7 cut(s) 342, 1190, 1384, 1436, 2200, 2546, 2856
FriOI GRGCYC 1 cut(s) 1812
FspBI CTAG 3 cut(s) 687, 1035, 1581
FspI TGCGCA 1 cut(s) 2171
GlaI GCGC 4 cut(s) 183, 890, 2171, 2195
GsaI CCCAGC 1 cut(s) 2896
GsuI CTGGAG 4 cut(s) 73, 901, 1116, 1650
HaeII RGCGCY 1 cut(s) 892
HaeIII GGCC 5 cut(s) 670, 1140, 1386, 1704, 1818
HapII CCGG 8 cut(s) 190, 202, 332, 671, 1705, 2499, 2706, 2889
HgaI GACGC 2 cut(s) 204, 2926
HhaI GCGC 4 cut(s) 184, 891, 2172, 2196
Hin1I GRCGYC 1 cut(s) 196
Hin6I GCGC 4 cut(s) 182, 889, 2170, 2194
HinP1I GCGC 4 cut(s) 182, 889, 2170, 2194
HindIII AAGCTT 3 cut(s) 1086, 1733, 2559
HpaII CCGG 8 cut(s) 190, 202, 332, 671, 1705, 2499, 2706, 2889
HphI GGTGA 5 cut(s) 239, 2657, 2663, 2669, 2822
Hpy188I TCNGA 8 cut(s) 156, 315, 1255, 2092, 2113, 2278, 2449, 2593
HpyAV CCTTC 9 cut(s) 67, 313, 496, 526, 607, 671, 1826, 2524, 2950
HpyCH4IV ACGT 2 cut(s) 9, 2734
HpySE526I ACGT 2 cut(s) 9, 2734
Hsp92I GRCGYC 1 cut(s) 196
HspAI GCGC 4 cut(s) 182, 889, 2170, 2194
KflI GGGWCCC 1 cut(s) 1001
Kpn2I TCCGGA 1 cut(s) 331
KroI GCCGGC 1 cut(s) 2888
KroNI GCCGGC 1 cut(s) 2890
Ksp22I TGATCA 1 cut(s) 244
Kzo9I GATC 6 cut(s) 244, 1255, 1686, 2684, 2738, 2788
LguI GCTCTTC 1 cut(s) 2193
LmnI GCTCC 6 cut(s) 92, 1291, 1315, 1446, 1669, 2143
Lsp1109I GCAGC 7 cut(s) 568, 720, 1093, 1645, 2032, 2164, 2953
LweI GCATC 9 cut(s) 319, 364, 673, 919, 1168, 2380, 2453, 2506, 2524
MaeI CTAG 3 cut(s) 687, 1035, 1581
MaeII ACGT 2 cut(s) 9, 2734
MaeIII GTNAC 7 cut(s) 813, 850, 864, 1715, 1721, 2651, 2748
MalI GATC 6 cut(s) 246, 1257, 1688, 2686, 2740, 2790
MboI GATC 6 cut(s) 244, 1255, 1686, 2684, 2738, 2788
MfeI CAATTG 1 cut(s) 2165
MflI RGATCY 1 cut(s) 1686
MhlI GDGCHC 1 cut(s) 1812
MlyI GAGTC 4 cut(s) 476, 500, 1109, 2323
MmeI TCCRAC 2 cut(s) 1313, 1337
Mph1103I ATGCAT 1 cut(s) 422
MroI TCCGGA 1 cut(s) 331
MroNI GCCGGC 1 cut(s) 2888
MroXI GAANNNNTTC 2 cut(s) 393, 2865
MseI TTAA 5 cut(s) 762, 962, 1238, 1334, 2360
MslI CAYNNNNRTG 4 cut(s) 101, 360, 753, 1422
MspCI CTTAAG 1 cut(s) 1237
MspI CCGG 8 cut(s) 190, 202, 332, 671, 1705, 2499, 2706, 2889
MspR9I CCNGG 4 cut(s) 1095, 1175, 1499, 2148
MunI CAATTG 1 cut(s) 2165
Mva1269I GAATGC 3 cut(s) 1938, 2214, 2365
MvaI CCWGG 4 cut(s) 1095, 1175, 1499, 2148
MvnI CGCG 1 cut(s) 184
NaeI GCCGGC 1 cut(s) 2890
NcoI CCATGG 2 cut(s) 706, 2197
NdeII GATC 6 cut(s) 244, 1255, 1686, 2684, 2738, 2788
NgoMIV GCCGGC 1 cut(s) 2888
NlaIV GGNNCC 5 cut(s) 188, 1002, 1003, 1503, 2145
NmeAIII GCCGAG 1 cut(s) 1609
NmuCI GTSAC 5 cut(s) 813, 850, 1721, 2651, 2748
NsbI TGCGCA 1 cut(s) 2171
NsiI ATGCAT 1 cut(s) 422
NspV TTCGAA 1 cut(s) 412
PaeR7I CTCGAG 1 cut(s) 1028
PagI TCATGA 2 cut(s) 1969, 1999
PaqCI CACCTGC 1 cut(s) 1795
PciSI GCTCTTC 1 cut(s) 2193
PctI GAATGC 3 cut(s) 1938, 2214, 2365
PdiI GCCGGC 1 cut(s) 2890
PdmI GAANNNNTTC 2 cut(s) 393, 2865
PflFI GACNNNGTC 2 cut(s) 490, 2763
PfoI TCCNGGA 2 cut(s) 1093, 1173
PleI GAGTC 4 cut(s) 475, 499, 1108, 2322
PpsI GAGTC 4 cut(s) 475, 499, 1108, 2322
Ppu21I YACGTR 1 cut(s) 10
PpuMI RGGWCCY 1 cut(s) 1001
PshAI GACNNNNGTC 1 cut(s) 1431
Psp5II RGGWCCY 1 cut(s) 1001
Psp6I CCWGG 4 cut(s) 1093, 1173, 1497, 2146
PspFI CCCAGC 1 cut(s) 2892
PspGI CCWGG 4 cut(s) 1093, 1173, 1497, 2146
PspN4I GGNNCC 5 cut(s) 188, 1002, 1003, 1503, 2145
PspPI GGNCC 4 cut(s) 1001, 1502, 2201, 2765
PspPPI RGGWCCY 1 cut(s) 1001
PsrI GAACNNNNNNTAC 2 cut(s) 2398, 2430
PstI CTGCAG 2 cut(s) 1327, 2050
PstNI CAGNNNCTG 1 cut(s) 2453
PsuI RGATCY 1 cut(s) 1686
PsyI GACNNNGTC 2 cut(s) 490, 2763
RsaI GTAC 5 cut(s) 263, 912, 925, 1224, 2423
RsaNI GTAC 5 cut(s) 262, 911, 924, 1223, 2422
RseI CAYNNNNRTG 4 cut(s) 101, 360, 753, 1422
SapI GCTCTTC 1 cut(s) 2193
SaqAI TTAA 5 cut(s) 762, 962, 1238, 1334, 2360
Sau3AI GATC 6 cut(s) 244, 1255, 1686, 2684, 2738, 2788
Sau96I GGNCC 4 cut(s) 1001, 1502, 2201, 2765
SchI GAGTC 4 cut(s) 476, 500, 1109, 2323
ScrFI CCNGG 4 cut(s) 1095, 1175, 1499, 2148
SduI GDGCHC 1 cut(s) 1812
SfaNI GCATC 9 cut(s) 319, 364, 673, 919, 1168, 2380, 2453, 2506, 2524
SfcI CTRYAG 4 cut(s) 78, 1323, 1927, 2046
Sfr274I CTCGAG 1 cut(s) 1028
SfuI TTCGAA 1 cut(s) 412
SinI GGWCC 4 cut(s) 1001, 1502, 2201, 2765
SlaI CTCGAG 1 cut(s) 1028
SmiMI CAYNNNNRTG 4 cut(s) 101, 360, 753, 1422
SmlI CTYRAG 6 cut(s) 125, 251, 401, 1028, 1237, 2951
SmoI CTYRAG 6 cut(s) 125, 251, 401, 1028, 1237, 2951
SspMI CTAG 3 cut(s) 687, 1035, 1581
StyD4I CCNGG 4 cut(s) 1093, 1173, 1497, 2146
StyI CCWWGG 3 cut(s) 706, 1141, 2197
TaiI ACGT 2 cut(s) 12, 2737
TaqI TCGA 7 cut(s) 73, 226, 412, 494, 903, 1029, 1052
TaqII GACCGA 1 cut(s) 558
TatI WGTACW 2 cut(s) 1222, 2421
TauI GCSGC 6 cut(s) 39, 187, 201, 219, 259, 2223
Tru1I TTAA 5 cut(s) 762, 962, 1238, 1334, 2360
Tru9I TTAA 5 cut(s) 762, 962, 1238, 1334, 2360
TscAI CASTG 4 cut(s) 822, 1527, 2485, 2914
TseFI GTSAC 5 cut(s) 813, 850, 1721, 2651, 2748
TseI GCWGC 7 cut(s) 556, 733, 1106, 1633, 2045, 2177, 2966
Tsp45I GTSAC 5 cut(s) 813, 850, 1721, 2651, 2748
TspGWI ACGGA 3 cut(s) 157, 279, 2697
TspRI CASTG 4 cut(s) 822, 1527, 2485, 2914
Tth111I GACNNNGTC 2 cut(s) 490, 2763
Vha464I CTTAAG 1 cut(s) 1237
VpaK11BI GGWCC 4 cut(s) 1001, 1502, 2201, 2765
XapI RAATTY 5 cut(s) 349, 1600, 1649, 2051, 2930
XbaI TCTAGA 1 cut(s) 1580
XhoI CTCGAG 1 cut(s) 1028
XmiI GTMKAC 2 cut(s) 1070, 2940
XmnI GAANNNNTTC 2 cut(s) 393, 2865
XspI CTAG 3 cut(s) 687, 1035, 1581
Zsp2I ATGCAT 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.