Rroxscaffold_2G00090670

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005671
Physical Location & Seq
Reverse (-)
12365750 .. 12370938
5189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00090670.1

Sequence Viewer

Length: 2880 bp
ATGGAGAAAAGAGAAAAGGTGAAGATGATAGTAGCGGCGGCCCTCGCTTCTCTCTCCGTCTTCTTCCTCTACAGGAGGAGACGCCGAAACTGCCCCCAAACTCACTGTTATTTACGAGACGACCCGAACAAGCCGCAGCACGGGTTCAAGCGAGTCTTGGCCGATAATTCCTACTCTCCGTTCAAGCATTTCAACCCCGCCGACGACTCTAACGTCGGCGGAGCAGATAAGAATTGGAATTCGCATCCTTACGAGGCGGAGATCACGGCGGTGATGGAAAGTCCGCCGATTGAGCTGAAATGTGTGACGGAGAATTTGGATTCGGAAATGAGGGAGGAGTATGTTTGGGTTGAGAATGAGACTCAGTTGAAGGAAATGGTTGAGGTTTTGAGTAGAGAGAAGATCTTTGGGGTTGATACTGAGCAGCATAGCTTAAGGTCATTTTTGGGATTTACTGCTTTGATACAGATTTCGACTGAGAAGGAAGATTATTTGGTGGACACTATTGCTTTACATGACAGTATGAGCCTTTTGCAGCCGGTTTTCGCCGATGCTAGTATCTGCAAGGTGTTCCATGGGGCAGATAGTGATGTTCTCTGGCTGCAAAGGGACTTTCATATTTATGTTGTCAATATGTTTGATACTGCAAAGGCTTGTGAAGTGTTGTCAAAACCGCAGAAATCACTGGCTTATTTACTTGAATCTTACTGTGGAGTCGTCACTAACAAATTGTTTCAGCGTGAAGACTGGAGACAACGCCCTTTGTCAATGGAAATGGTACAATATGCTCGGACAGATGCACACTATCTACTTTACATTGCAGGCCGTTTACTAGCTGAGCTGGATGTGCTGGATAATGAAAACTCTTGTCCCAATGACAAATTCCGTTTTGTTCTCGAGGCTAGTCGGCGTTCAAACATGATATGTTTGCAACTGTATACGAAAGACATAGAAGCTTCGCCTGGAGAGTCTGCTGCTTCATCAATATTTAGCCGTCAATTAAATGGCCAAGGAGGCATTTCATCAGCATCTTATGAAATCCAGAATTCTGTGAGACGACTTTGTGCATGGAGAGATTTGATGGCTCGTGTACATGATGAGAGCTTGAGATATGTAATATCAGATCAGGCTATTGTTGCCCTTGCAGAAAAAGCTCCAACTAACCCTGCAGACATGTTAACCACCATTGCAGAAGCTGATGATAATGTGGATTCAAGTTTCAATTCTGGCCTCTCATCTCCCTCTCCTGTGGTCTGCAGTCATTTGAATGATTTTCATTATCTGCTCCAGAACAAAGTAGGCAATCCTGATGATCTGTTACCCATGATTCTTCAGAACTGCCTGGGTCAAAAAGGGAGTTGCCCACTGTCTGTCTTCAACTATGCTTTACTGGTTAATTATAATATGAAAGTACCACTGGTTTCTAAACAAAATGGGGTCAGAAATCCAAAGCAAGTCGGTCGAATGGCTTCGCGAAAGCTGTTTGTTCAAAAATTTTCCTGCAAATCCCCAGTTTATCATAATTGCAGGATCTATGCAAATGATGGGCGTTTGCTGTGTTACTGTGACCGCAGAAAGCTTGAATGGTATCTTCATCGGAATTTGGCTAAACTTGTTGAGGACAATCCACCTGGAATTATGCTTCTTTTTGAGCCCAAAGGCCGTCCAGAAGATGAAGGCAATGATTTCTACATTCAAAGTAAGAAAAATATATGTGTTGGCTGCGGTGAAGGAAATCATTACTTACGGTACCGAATTATTCCCTCATGCTACAGAATGCACTTCCCTGAACATTTGAAAAGCCACCGTTCTCATGATATTGTCCTACTATGCGTGGACTGTCACGAAATTGCTCATGCCGCTGCGGAGAAGTATAAAAGAAAGATTGCTGCAGAATTTGGGATTCCACTTTTTGTTCGTAAAGTGGTTGATTCGCAACAAGCTCAATCTTTATCTGGGGCATCTGTGCCTGCAACAAATGTTGAGGAAGCAGGTGTATCTCCTTTACAGTTGCGAACTGCTGCTATGGCTCTTTTGCGCCATGGATCAACAATGCCATCCAAGCGCCGTGAGGAACTGACAGAGATTGTTATGACATTTTATGGAGGGAGGCAAATCTCTGAGGAAGATTTAGAAAAGGCTTTGCTTGTTGGGATGACTCCTCATGAGAAAAGACGACTTCAGAAGAAGAAAGGGTTTTCTTTTCAACATTCTAGGGGGAGTATTTCTTCATATGCAGAGGAGAAAATTACTGTTAACATGTCCACATCTGCCACTCCAGATGCTTCAGAAGTTGATACTGTACGTGAATCTTGCACCCATGAAGCCAAAGCATCTATGATAAACCAAGATGAAGATGTTCTCACAGTGAAGGATGCTGATTTAGGCAACTCCGCTTTAGATTTGAGGATTGGTGAAAAAGCATCTCCAAGCAATGGGAAGAACATGAGTTCAGAGATAACCAGAGCGTCCGATACTACACTTGTTTCTGTTGTAAATGGTGATGGTATTTATGACGTTAGAAGTACACCAAATGGATCAGTAGACTTCCGTTCTACGTCCAAACGTAATCATAAATCAAAACACTCCCTCTTAGGACATGGTCCACATGGAAGGCAAGTTGTGGATCATCTGCTAAGGGAATCTGGAGAAGATGGTATTAGTCAGTTCTGTCAAAGGTGGAGACAAGTATTTGTTGAAACCGTTCATCCCAGTTTTTACCTGCCGGCTGGGATATTACACACAGCTTCTGAAGTTTTGTTTATCTGTGCCAAGGGTTTGATTAAGGGCACTGTTTTTTCCAGTGGTAGGAGGGATTTTGGAGAATTTAGCGTCTACAATCCTACCAAGAAAGCTTCTGCTGTTACTTCAAGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0000178 GO:0000460 GO:0000785 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006403 GO:0006725 GO:0006807 GO:0007549 GO:0008150 GO:0008152 GO:0008298 GO:0008334 GO:0008408 GO:0009048 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010639 GO:0016070 GO:0016071 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032210 GO:0032211 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035327 GO:0040029 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045934 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051179 GO:0051641 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071025 GO:0071027 GO:0071028 GO:0071029 GO:0071030 GO:0071033 GO:0071034 GO:0071035 GO:0071043 GO:0071044 GO:0071046 GO:0071048 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902494 GO:1904356 GO:1904357 GO:1904872 GO:1905354 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1 HRDC

Protein Analysis

959

Amino Acids

107.91

Weight (kDa)

7.03

Isoelectric Point (pI)

51.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 114 - 281 4.7e-50 3'-5' exonuclease
HRDC PF00570 351 - 394 1.3e-09 HRDC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1401
AarI CACCTGC 1 cut(s) 1982
AasI GACNNNNNNGTC 1 cut(s) 212
Acc36I ACCTGC 2 cut(s) 1982, 2730
Acc65I GGTACC 1 cut(s) 1749
AccB1I GGYRCC 1 cut(s) 1749
AccB7I CCANNNNNTGG 1 cut(s) 2435
AccI GTMKAC 3 cut(s) 938, 2544, 2835
AccII CGCG 1 cut(s) 1474
AclWI GGATC 4 cut(s) 1538, 2053, 2545, 2634
AcoI YGGCCR 2 cut(s) 159, 1006
AcsI RAATTY 8 cut(s) 238, 313, 881, 1045, 1493, 1600, 1895, 2825
AcuI CTGAAG 4 cut(s) 1316, 2165, 2271, 2772
AcyI GRCGYC 1 cut(s) 82
AfaI GTAC 6 cut(s) 780, 1092, 1413, 1751, 2304, 2527
AfiI CCNNNNNNNGG 3 cut(s) 140, 2435, 2808
AflII CTTAAG 1 cut(s) 433
AflIII ACRYGT 2 cut(s) 1173, 2259
AjnI CCWGG 3 cut(s) 961, 1341, 1630
AjuI GAANNNNNNNTTGG 2 cut(s) 866, 898
AleI CACNNNNGTG 1 cut(s) 269
Alw26I GTCTC 6 cut(s) 73, 111, 353, 745, 1048, 2677
AlwI GGATC 4 cut(s) 1538, 2053, 2545, 2634
AlwNI CAGNNNCTG 2 cut(s) 1196, 2750
Ama87I CYCGRG 1 cut(s) 896
AoxI GGCC 6 cut(s) 39, 159, 823, 1006, 1228, 1660
ApeKI GCWGC 9 cut(s) 136, 424, 535, 601, 974, 1722, 1862, 1889, 2021
ApoI RAATTY 8 cut(s) 238, 313, 881, 1045, 1493, 1600, 1895, 2825
ArsI GACNNNNNNTTYG 2 cut(s) 298, 330
Asp700I GAANNNNTTC 3 cut(s) 1468, 2358, 2703
Asp718I GGTACC 1 cut(s) 1749
AspLEI GCGC 2 cut(s) 2040, 2067
AspS9I GGNCC 2 cut(s) 40, 2603
AsuHPI GGTGA 5 cut(s) 31, 283, 1739, 2426, 2513
AvaI CYCGRG 1 cut(s) 896
AvaII GGWCC 1 cut(s) 2603
BaeGI GKGCMC 1 cut(s) 2792
BalI TGGCCA 1 cut(s) 1008
BanI GGYRCC 1 cut(s) 1749
BanII GRGCYC 1 cut(s) 1656
BauI CACGAG 1 cut(s) 1086
BbsI GAAGAC 3 cut(s) 52, 750, 1366
BbvI GCAGC 9 cut(s) 148, 436, 547, 588, 961, 1709, 1849, 1876, 2008
BccI CCATC 6 cut(s) 268, 1075, 1538, 2065, 2498, 2648
BceAI ACGGC 5 cut(s) 282, 810, 978, 1647, 2052
BcgI CGANNNNNNTGC 2 cut(s) 1442, 1476
BciT130I CCWGG 3 cut(s) 963, 1343, 1632
BcoDI GTCTC 6 cut(s) 73, 111, 353, 745, 1048, 2677
BfaI CTAG 4 cut(s) 555, 833, 903, 2214
BfmI CTRYAG 5 cut(s) 70, 1167, 1255, 1771, 1890
BfoI RGCGCY 1 cut(s) 2068
BfrI CTTAAG 1 cut(s) 433
BfuAI ACCTGC 2 cut(s) 1982, 2730
BglI GCCNNNNNGGC 1 cut(s) 1014
BglII AGATCT 1 cut(s) 402
BlpI GCTNAGC 1 cut(s) 837
Bme1390I CCNGG 3 cut(s) 963, 1343, 1632
Bme18I GGWCC 1 cut(s) 2603
BmeT110I CYCGRG 1 cut(s) 896
BmgT120I GGNCC 2 cut(s) 40, 2603
BmiI GGNNCC 1 cut(s) 1751
BmrFI CCNGG 3 cut(s) 963, 1343, 1632
BmrI ACTGGG 2 cut(s) 1505, 2706
BmsI GCATC 9 cut(s) 253, 541, 787, 1037, 1970, 2272, 2342, 2365, 2432
BmuI ACTGGG 2 cut(s) 1505, 2706
BpiI GAAGAC 3 cut(s) 52, 750, 1366
BpmI CTGGAG 5 cut(s) 769, 984, 1271, 2262, 2667
Bpu10I CCTNAGC 1 cut(s) 2636
Bpu1102I GCTNAGC 1 cut(s) 837
BpuEI CTTGAG 1 cut(s) 1126
BsaAI YACGTR 1 cut(s) 2306
BsaHI GRCGYC 1 cut(s) 82
BsaJI CCNNGG 5 cut(s) 574, 1009, 1342, 2041, 2772
Bsc4I CCNNNNNNNGG 3 cut(s) 140, 2435, 2808
Bse118I RCCGGY 2 cut(s) 538, 2725
Bse1I ACTGG 7 cut(s) 690, 752, 1395, 1422, 1511, 2712, 2802
Bse3DI GCAATG 4 cut(s) 816, 1185, 1687, 2440
BseBI CCWGG 3 cut(s) 963, 1343, 1632
BseDI CCNNGG 5 cut(s) 574, 1009, 1342, 2041, 2772
BseGI GGATG 6 cut(s) 244, 850, 2057, 2160, 2380, 2707
BseLI CCNNNNNNNGG 3 cut(s) 140, 2435, 2808
BseMI GCAATG 4 cut(s) 816, 1185, 1687, 2440
BseMII CTCAG 5 cut(s) 377, 411, 468, 828, 2112
BseNI ACTGG 7 cut(s) 690, 752, 1395, 1422, 1511, 2712, 2802
BseRI GAGGAG 4 cut(s) 91, 350, 2151, 2255
BseSI GKGCMC 1 cut(s) 2792
BseXI GCAGC 9 cut(s) 148, 436, 547, 588, 961, 1709, 1849, 1876, 2008
BseYI CCCAGC 1 cut(s) 2729
Bsh1236I CGCG 1 cut(s) 1474
Bsh1285I CGRYCG 1 cut(s) 1462
BshFI GGCC 6 cut(s) 41, 161, 825, 1008, 1230, 1662
BshNI GGYRCC 1 cut(s) 1749
BsiEI CGRYCG 1 cut(s) 1462
BsiHKCI CYCGRG 1 cut(s) 896
BsiSI CCGG 2 cut(s) 539, 2726
BslFI GGGAC 2 cut(s) 623, 855
BslI CCNNNNNNNGG 3 cut(s) 140, 2435, 2808
BsmAI GTCTC 6 cut(s) 73, 111, 353, 745, 1048, 2677
BsmBI CGTCTC 3 cut(s) 73, 111, 1048
BsmFI GGGAC 2 cut(s) 623, 855
BsmI GAATGC 1 cut(s) 1782
BsnI GGCC 6 cut(s) 41, 161, 825, 1008, 1230, 1662
BsoBI CYCGRG 1 cut(s) 896
Bsp1286I GDGCHC 2 cut(s) 1656, 2792
Bsp1407I TGTACA 1 cut(s) 1090
Bsp143I GATC 8 cut(s) 261, 402, 1123, 1312, 1530, 2045, 2537, 2626
Bsp1720I GCTNAGC 1 cut(s) 837
Bsp19I CCATGG 2 cut(s) 574, 2041
Bsp68I TCGCGA 1 cut(s) 1474
BspANI GGCC 6 cut(s) 41, 161, 825, 1008, 1230, 1662
BspCNI CTCAG 5 cut(s) 376, 412, 469, 829, 2113
BspFNI CGCG 1 cut(s) 1474
BspHI TCATGA 2 cut(s) 1813, 2164
BspLI GGNNCC 1 cut(s) 1751
BspMAI CTGCAG 3 cut(s) 1171, 1259, 1894
BspMI ACCTGC 2 cut(s) 1982, 2730
BspPI GGATC 4 cut(s) 1538, 2053, 2545, 2634
BspT107I GGYRCC 1 cut(s) 1749
BspTI CTTAAG 1 cut(s) 433
BsrDI GCAATG 4 cut(s) 816, 1185, 1687, 2440
BsrFI RCCGGY 2 cut(s) 538, 2725
BsrGI TGTACA 1 cut(s) 1090
BsrI ACTGG 7 cut(s) 690, 752, 1395, 1422, 1511, 2712, 2802
BssAI RCCGGY 2 cut(s) 538, 2725
BssECI CCNNGG 5 cut(s) 574, 1009, 1342, 2041, 2772
BssMI GATC 8 cut(s) 261, 402, 1123, 1312, 1530, 2045, 2537, 2626
BssNAI GTATAC 1 cut(s) 939
BssNI GRCGYC 1 cut(s) 82
BssSI CACGAG 1 cut(s) 1086
BssT1I CCWWGG 4 cut(s) 574, 1009, 2041, 2772
Bst1107I GTATAC 1 cut(s) 939
Bst2BI CACGAG 1 cut(s) 1086
Bst2UI CCWGG 3 cut(s) 963, 1343, 1632
BstACI GRCGYC 1 cut(s) 82
BstAFI CTTAAG 1 cut(s) 433
BstAUI TGTACA 1 cut(s) 1090
BstBAI YACGTR 1 cut(s) 2306
BstC8I GCNNGC 3 cut(s) 823, 1971, 2727
BstDEI CTNAG 7 cut(s) 363, 420, 477, 837, 2121, 2593, 2636
BstDSI CCRYGG 2 cut(s) 574, 2041
BstF5I GGATG 6 cut(s) 244, 850, 2057, 2160, 2380, 2707
BstFNI CGCG 1 cut(s) 1474
BstH2I RGCGCY 1 cut(s) 2068
BstHHI GCGC 2 cut(s) 2040, 2067
BstKTI GATC 8 cut(s) 264, 405, 1126, 1315, 1533, 2048, 2540, 2629
BstMAI GTCTC 6 cut(s) 73, 111, 353, 745, 1048, 2677
BstMBI GATC 8 cut(s) 261, 402, 1123, 1312, 1530, 2045, 2537, 2626
BstMCI CGRYCG 1 cut(s) 1462
BstNI CCWGG 3 cut(s) 963, 1343, 1632
BstNSI RCATGY 2 cut(s) 1177, 2263
BstSCI CCNGG 3 cut(s) 961, 1341, 1630
BstSFI CTRYAG 5 cut(s) 70, 1167, 1255, 1771, 1890
BstSLI GKGCMC 1 cut(s) 2792
BstUI CGCG 1 cut(s) 1474
BstV1I GCAGC 9 cut(s) 148, 436, 547, 588, 961, 1709, 1849, 1876, 2008
BstV2I GAAGAC 3 cut(s) 52, 750, 1366
BstX2I RGATCY 2 cut(s) 402, 1530
BstYI RGATCY 2 cut(s) 402, 1530
BstZ17I GTATAC 1 cut(s) 939
BsuRI GGCC 6 cut(s) 41, 161, 825, 1008, 1230, 1662
BtgI CCRYGG 2 cut(s) 574, 2041
BtsCI GGATG 6 cut(s) 244, 850, 2057, 2160, 2380, 2707
BtsIMutI CAGTG 7 cut(s) 103, 683, 1364, 1415, 2373, 2790, 2809
BtuMI TCGCGA 1 cut(s) 1474
BveI ACCTGC 2 cut(s) 1982, 2730
Cac8I GCNNGC 3 cut(s) 823, 1971, 2727
CaiI CAGNNNCTG 2 cut(s) 1196, 2750
CciI TCATGA 2 cut(s) 1813, 2164
CfoI GCGC 2 cut(s) 2040, 2067
Cfr10I RCCGGY 2 cut(s) 538, 2725
Cfr13I GGNCC 2 cut(s) 40, 2603
CseI GACGC 3 cut(s) 90, 2457, 2821
Csp6I GTAC 6 cut(s) 779, 1091, 1412, 1750, 2303, 2526
CviQI GTAC 6 cut(s) 779, 1091, 1412, 1750, 2303, 2526
DdeI CTNAG 7 cut(s) 363, 420, 477, 837, 2121, 2593, 2636
DpnI GATC 8 cut(s) 263, 404, 1125, 1314, 1532, 2047, 2539, 2628
DpnII GATC 8 cut(s) 261, 402, 1123, 1312, 1530, 2045, 2537, 2626
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
EaeI YGGCCR 2 cut(s) 159, 1006
EciI GGCGGA 3 cut(s) 234, 272, 273
Eco130I CCWWGG 4 cut(s) 574, 1009, 2041, 2772
Eco24I GRGCYC 1 cut(s) 1656
Eco47I GGWCC 1 cut(s) 2603
Eco57I CTGAAG 4 cut(s) 1316, 2165, 2271, 2772
Eco88I CYCGRG 1 cut(s) 896
EcoRI GAATTC 2 cut(s) 238, 1045
EcoRII CCWGG 3 cut(s) 961, 1341, 1630
EcoT14I CCWWGG 4 cut(s) 574, 1009, 2041, 2772
EcoT38I GRGCYC 1 cut(s) 1656
ErhI CCWWGG 4 cut(s) 574, 1009, 2041, 2772
Esp3I CGTCTC 3 cut(s) 73, 111, 1048
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FaqI GGGAC 2 cut(s) 623, 855
FauI CCCGC 1 cut(s) 205
FauNDI CATATG 1 cut(s) 2233
FblI GTMKAC 3 cut(s) 938, 2544, 2835
FokI GGATG 6 cut(s) 231, 857, 2044, 2167, 2387, 2694
FriOI GRGCYC 1 cut(s) 1656
FspBI CTAG 4 cut(s) 555, 833, 903, 2214
GlaI GCGC 2 cut(s) 2039, 2066
GsaI CCCAGC 1 cut(s) 2733
GsuI CTGGAG 5 cut(s) 769, 984, 1271, 2262, 2667
HaeII RGCGCY 1 cut(s) 2068
HaeIII GGCC 6 cut(s) 41, 161, 825, 1008, 1230, 1662
HapII CCGG 2 cut(s) 539, 2726
HgaI GACGC 3 cut(s) 90, 2457, 2821
HhaI GCGC 2 cut(s) 2040, 2067
Hin1I GRCGYC 1 cut(s) 82
Hin6I GCGC 2 cut(s) 2038, 2065
HinP1I GCGC 2 cut(s) 2038, 2065
HincII GTYRAC 2 cut(s) 1179, 2257
HindII GTYRAC 2 cut(s) 1179, 2257
HindIII AAGCTT 3 cut(s) 954, 1577, 2853
HpaI GTTAAC 2 cut(s) 1179, 2257
HpaII CCGG 2 cut(s) 539, 2726
HphI GGTGA 5 cut(s) 31, 283, 1739, 2426, 2513
Hpy99I CGWCG 2 cut(s) 206, 218
HpyAV CCTTC 6 cut(s) 364, 475, 1670, 1724, 2365, 2607
HpyCH4IV ACGT 5 cut(s) 213, 2305, 2517, 2558, 2566
HpyF3I CTNAG 7 cut(s) 363, 420, 477, 837, 2121, 2593, 2636
HpySE526I ACGT 5 cut(s) 213, 2305, 2517, 2558, 2566
Hsp92I GRCGYC 1 cut(s) 82
HspAI GCGC 2 cut(s) 2038, 2065
KpnI GGTACC 1 cut(s) 1753
KroI GCCGGC 1 cut(s) 2725
KroNI GCCGGC 1 cut(s) 2727
KspAI GTTAAC 2 cut(s) 1179, 2257
Kzo9I GATC 8 cut(s) 261, 402, 1123, 1312, 1530, 2045, 2537, 2626
LmnI GCTCC 3 cut(s) 221, 1159, 1290
Lsp1109I GCAGC 9 cut(s) 148, 436, 547, 588, 961, 1709, 1849, 1876, 2008
LweI GCATC 9 cut(s) 253, 541, 787, 1037, 1970, 2272, 2342, 2365, 2432
MaeI CTAG 4 cut(s) 555, 833, 903, 2214
MaeII ACGT 5 cut(s) 213, 2305, 2517, 2558, 2566
MaeIII GTNAC 7 cut(s) 304, 718, 1317, 1559, 1565, 1841, 2863
MalI GATC 8 cut(s) 263, 404, 1125, 1314, 1532, 2047, 2539, 2628
MboI GATC 8 cut(s) 261, 402, 1123, 1312, 1530, 2045, 2537, 2626
MflI RGATCY 2 cut(s) 402, 1530
MhlI GDGCHC 2 cut(s) 1656, 2792
MlsI TGGCCA 1 cut(s) 1008
MluNI TGGCCA 1 cut(s) 1008
MlyI GAGTC 6 cut(s) 162, 200, 355, 723, 977, 2152
MmeI TCCRAC 1 cut(s) 1181
Mox20I TGGCCA 1 cut(s) 1008
MroNI GCCGGC 1 cut(s) 2725
MroXI GAANNNNTTC 3 cut(s) 1468, 2358, 2703
MscI TGGCCA 1 cut(s) 1008
MseI TTAA 6 cut(s) 434, 1001, 1178, 1395, 2256, 2784
MslI CAYNNNNRTG 3 cut(s) 269, 621, 1266
Msp20I TGGCCA 1 cut(s) 1008
MspA1I CMGCKG 1 cut(s) 1862
MspCI CTTAAG 1 cut(s) 433
MspI CCGG 2 cut(s) 539, 2726
MspR9I CCNGG 3 cut(s) 963, 1343, 1632
Mva1269I GAATGC 1 cut(s) 1782
MvaI CCWGG 3 cut(s) 963, 1343, 1632
MvnI CGCG 1 cut(s) 1474
NaeI GCCGGC 1 cut(s) 2727
NcoI CCATGG 2 cut(s) 574, 2041
NdeI CATATG 1 cut(s) 2233
NdeII GATC 8 cut(s) 261, 402, 1123, 1312, 1530, 2045, 2537, 2626
NgoMIV GCCGGC 1 cut(s) 2725
NlaIV GGNNCC 1 cut(s) 1751
NmuCI GTSAC 4 cut(s) 304, 718, 1565, 1841
NruI TCGCGA 1 cut(s) 1474
NspI RCATGY 2 cut(s) 1177, 2263
OliI CACNNNNGTG 1 cut(s) 269
PaeR7I CTCGAG 1 cut(s) 896
PagI TCATGA 2 cut(s) 1813, 2164
PaqCI CACCTGC 1 cut(s) 1982
PciI ACATGT 2 cut(s) 1173, 2259
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PctI GAATGC 1 cut(s) 1782
PdiI GCCGGC 1 cut(s) 2727
PdmI GAANNNNTTC 3 cut(s) 1468, 2358, 2703
PfeI GAWTC 8 cut(s) 320, 701, 1211, 1327, 1903, 1931, 2309, 2642
PflFI GACNNNGTC 1 cut(s) 2601
PflMI CCANNNNNTGG 1 cut(s) 2435
PleI GAGTC 6 cut(s) 161, 200, 355, 722, 976, 2152
PpsI GAGTC 6 cut(s) 161, 200, 355, 722, 976, 2152
Ppu21I YACGTR 1 cut(s) 2306
PscI ACATGT 2 cut(s) 1173, 2259
PsiI TTATAA 1 cut(s) 1401
Psp6I CCWGG 3 cut(s) 961, 1341, 1630
PspFI CCCAGC 1 cut(s) 2729
PspGI CCWGG 3 cut(s) 961, 1341, 1630
PspN4I GGNNCC 1 cut(s) 1751
PspPI GGNCC 2 cut(s) 40, 2603
PstI CTGCAG 3 cut(s) 1171, 1259, 1894
PstNI CAGNNNCTG 2 cut(s) 1196, 2750
PsuI RGATCY 2 cut(s) 402, 1530
PsyI GACNNNGTC 1 cut(s) 2601
RruI TCGCGA 1 cut(s) 1474
RsaI GTAC 6 cut(s) 780, 1092, 1413, 1751, 2304, 2527
RsaNI GTAC 6 cut(s) 779, 1091, 1412, 1750, 2303, 2526
RseI CAYNNNNRTG 3 cut(s) 269, 621, 1266
SaqAI TTAA 6 cut(s) 434, 1001, 1178, 1395, 2256, 2784
Sau3AI GATC 8 cut(s) 261, 402, 1123, 1312, 1530, 2045, 2537, 2626
Sau96I GGNCC 2 cut(s) 40, 2603
SchI GAGTC 6 cut(s) 162, 200, 355, 723, 977, 2152
ScrFI CCNGG 3 cut(s) 963, 1343, 1632
SduI GDGCHC 2 cut(s) 1656, 2792
SfaNI GCATC 9 cut(s) 253, 541, 787, 1037, 1970, 2272, 2342, 2365, 2432
SfcI CTRYAG 5 cut(s) 70, 1167, 1255, 1771, 1890
Sfr274I CTCGAG 1 cut(s) 896
SinI GGWCC 1 cut(s) 2603
SlaI CTCGAG 1 cut(s) 896
SmiMI CAYNNNNRTG 3 cut(s) 269, 621, 1266
SmlI CTYRAG 3 cut(s) 433, 896, 1105
SmoI CTYRAG 3 cut(s) 433, 896, 1105
SspI AATATT 1 cut(s) 987
SspMI CTAG 4 cut(s) 555, 833, 903, 2214
StyD4I CCNGG 3 cut(s) 961, 1341, 1630
StyI CCWWGG 4 cut(s) 574, 1009, 2041, 2772
TaiI ACGT 5 cut(s) 216, 2308, 2520, 2561, 2569
TaqI TCGA 3 cut(s) 473, 897, 1462
TaqII GACCGA 1 cut(s) 1448
TatI WGTACW 2 cut(s) 1090, 2525
TauI GCSGC 4 cut(s) 38, 41, 136, 1862
TfiI GAWTC 8 cut(s) 320, 701, 1211, 1327, 1903, 1931, 2309, 2642
Tru1I TTAA 6 cut(s) 434, 1001, 1178, 1395, 2256, 2784
Tru9I TTAA 6 cut(s) 434, 1001, 1178, 1395, 2256, 2784
TscAI CASTG 7 cut(s) 110, 690, 1371, 1422, 2373, 2797, 2809
TseFI GTSAC 4 cut(s) 304, 718, 1565, 1841
TseI GCWGC 9 cut(s) 136, 424, 535, 601, 974, 1722, 1862, 1889, 2021
Tsp45I GTSAC 4 cut(s) 304, 718, 1565, 1841
TspGWI ACGGA 5 cut(s) 46, 168, 323, 875, 2540
TspRI CASTG 7 cut(s) 110, 690, 1371, 1422, 2373, 2797, 2809
Tth111I GACNNNGTC 1 cut(s) 2601
Van91I CCANNNNNTGG 1 cut(s) 2435
Vha464I CTTAAG 1 cut(s) 433
VpaK11BI GGWCC 1 cut(s) 2603
XapI RAATTY 8 cut(s) 238, 313, 881, 1045, 1493, 1600, 1895, 2825
XceI RCATGY 2 cut(s) 1177, 2263
XhoI CTCGAG 1 cut(s) 896
XmiI GTMKAC 3 cut(s) 938, 2544, 2835
XmnI GAANNNNTTC 3 cut(s) 1468, 2358, 2703
XspI CTAG 4 cut(s) 555, 833, 903, 2214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.