Rorug02G0475400

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005671
Physical Location & Seq
Forward (+)
60665312 .. 60665704
393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0475400.1

Sequence Viewer

Length: 393 bp
ATGGACGAGATGGCAGGGAGCGGTAGTGTCCGTTTCTCTTCCTCAGATTGGCTTGTCTGGGATCGTTCAGATCCGATGGTGAGGAAGGTGATTTGGAGTTGCTCGAATTGGGGTCTGATGAAGAGTTGTGCCCCGGATGGTGGTTACGCTATCAGTGGCCGGTGCTCCCAAGGATTCTGGCTGTGGAATAAACGGTACTGCGGCGGCCTCAACAGCTACCGTACGACTGGAGTTCAGCGGCGGTGCTTAGGAGTCTCAATTGGATTCTCTTGGACTATCGGAAGCCCCCGACCTCAGCGCAGCTTGCATGGTGGTGCAGGTGTCAGCGGCGGCAAAACTAGGGATGGCAAAAATCCCCAGCGGGGCGGAGCTCCGTTGGATACCCGCCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0000178 GO:0000460 GO:0000785 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006403 GO:0006725 GO:0006807 GO:0007549 GO:0008150 GO:0008152 GO:0008298 GO:0008334 GO:0008408 GO:0009048 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010639 GO:0016070 GO:0016071 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032210 GO:0032211 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035327 GO:0040029 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045934 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051179 GO:0051641 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071025 GO:0071027 GO:0071028 GO:0071029 GO:0071030 GO:0071033 GO:0071034 GO:0071035 GO:0071043 GO:0071044 GO:0071046 GO:0071048 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902494 GO:1904356 GO:1904357 GO:1904872 GO:1905354 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1 HRDC

Protein Analysis

130

Amino Acids

14.16

Weight (kDa)

10.06

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 308
Acc36I ACCTGC 1 cut(s) 308
AccBSI CCGCTC 1 cut(s) 21
AclWI GGATC 2 cut(s) 65, 69
AcoI YGGCCR 1 cut(s) 157
AfaI GTAC 2 cut(s) 197, 223
AfiI CCNNNNNNNGG 3 cut(s) 48, 140, 362
AluBI AGCT 3 cut(s) 216, 303, 371
AluI AGCT 3 cut(s) 216, 303, 371
Alw21I GWGCWC 2 cut(s) 167, 373
Alw26I GTCTC 1 cut(s) 259
AlwI GGATC 2 cut(s) 65, 69
AoxI GGCC 2 cut(s) 157, 205
ApeKI GCWGC 1 cut(s) 300
AspLEI GCGC 1 cut(s) 300
AsuC2I CCSGG 1 cut(s) 134
AsuHPI GGTGA 2 cut(s) 91, 100
BaeGI GKGCMC 1 cut(s) 133
BanII GRGCYC 1 cut(s) 373
Bbv12I GWGCWC 2 cut(s) 167, 373
BbvCI CCTCAGC 1 cut(s) 294
BbvI GCAGC 1 cut(s) 312
BccI CCATC 4 cut(s) 4, 70, 131, 338
BciVI GTATCC 1 cut(s) 373
BcnI CCSGG 1 cut(s) 134
BcoDI GTCTC 1 cut(s) 259
BfaI CTAG 1 cut(s) 339
BfuAI ACCTGC 1 cut(s) 308
BfuI GTATCC 1 cut(s) 373
BisI GCNGC 6 cut(s) 202, 205, 239, 301, 328, 331
BlsI GCNGC 6 cut(s) 203, 206, 240, 302, 329, 332
Bme1390I CCNGG 1 cut(s) 134
BmrFI CCNGG 1 cut(s) 134
BpmI CTGGAG 1 cut(s) 249
Bpu10I CCTNAGC 2 cut(s) 247, 294
BpuMI CCSGG 1 cut(s) 134
BsaJI CCNNGG 2 cut(s) 132, 169
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 140, 362
Bse118I RCCGGY 1 cut(s) 159
Bse1I ACTGG 1 cut(s) 232
BseDI CCNNGG 2 cut(s) 132, 169
BseGI GGATG 2 cut(s) 142, 349
BseLI CCNNNNNNNGG 3 cut(s) 48, 140, 362
BseMII CTCAG 2 cut(s) 57, 308
BseNI ACTGG 1 cut(s) 232
BseSI GKGCMC 1 cut(s) 133
BseXI GCAGC 1 cut(s) 312
BseYI CCCAGC 1 cut(s) 357
BsgI GTGCAG 1 cut(s) 336
BshFI GGCC 2 cut(s) 159, 207
BsiHKAI GWGCWC 2 cut(s) 167, 373
BsiSI CCGG 2 cut(s) 134, 160
BsiWI CGTACG 1 cut(s) 221
BslI CCNNNNNNNGG 3 cut(s) 48, 140, 362
BsmAI GTCTC 1 cut(s) 259
BsnI GGCC 2 cut(s) 159, 207
Bsp1286I GDGCHC 3 cut(s) 133, 167, 373
Bsp143I GATC 2 cut(s) 61, 70
BspANI GGCC 2 cut(s) 159, 207
BspCNI CTCAG 2 cut(s) 56, 307
BspMI ACCTGC 1 cut(s) 308
BspPI GGATC 2 cut(s) 65, 69
BsrBI CCGCTC 1 cut(s) 21
BsrFI RCCGGY 1 cut(s) 159
BsrI ACTGG 1 cut(s) 232
BssAI RCCGGY 1 cut(s) 159
BssECI CCNNGG 2 cut(s) 132, 169
BssMI GATC 2 cut(s) 61, 70
BssT1I CCWWGG 1 cut(s) 169
Bst4CI ACNGT 2 cut(s) 195, 221
Bst6I CTCTTC 2 cut(s) 43, 116
BstC8I GCNNGC 1 cut(s) 305
BstDEI CTNAG 3 cut(s) 43, 247, 294
BstF5I GGATG 2 cut(s) 142, 349
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 2 cut(s) 64, 73
BstMAI GTCTC 1 cut(s) 259
BstMBI GATC 2 cut(s) 61, 70
BstMWI GCNNNNNNNGC 2 cut(s) 213, 304
BstSCI CCNGG 1 cut(s) 132
BstSLI GKGCMC 1 cut(s) 133
BstV1I GCAGC 1 cut(s) 312
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
BsuI GTATCC 1 cut(s) 373
BsuRI GGCC 2 cut(s) 159, 207
BtsCI GGATG 2 cut(s) 142, 349
BtsIMutI CAGTG 1 cut(s) 160
BveI ACCTGC 1 cut(s) 308
Cac8I GCNNGC 1 cut(s) 305
CfoI GCGC 1 cut(s) 300
Cfr10I RCCGGY 1 cut(s) 159
Csp6I GTAC 2 cut(s) 196, 222
CviAII CATG 1 cut(s) 308
CviJI RGCY 8 cut(s) 52, 159, 181, 207, 216, 285, 303, 371
CviKI_1 RGCY 8 cut(s) 52, 159, 181, 207, 216, 285, 303, 371
CviQI GTAC 2 cut(s) 196, 222
DdeI CTNAG 3 cut(s) 43, 247, 294
DpnI GATC 2 cut(s) 63, 72
DpnII GATC 2 cut(s) 61, 70
EaeI YGGCCR 1 cut(s) 157
Eam1104I CTCTTC 2 cut(s) 43, 116
EarI CTCTTC 2 cut(s) 43, 116
EciI GGCGGA 1 cut(s) 381
Ecl136II GAGCTC 1 cut(s) 371
Eco130I CCWWGG 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 373
Eco53kI GAGCTC 1 cut(s) 371
EcoICRI GAGCTC 1 cut(s) 371
EcoT14I CCWWGG 1 cut(s) 169
EcoT38I GRGCYC 1 cut(s) 373
ErhI CCWWGG 1 cut(s) 169
FaeI CATG 1 cut(s) 311
FaiI YATR 1 cut(s) 309
FatI CATG 1 cut(s) 307
FauI CCCGC 1 cut(s) 354
Fnu4HI GCNGC 6 cut(s) 202, 205, 239, 301, 328, 331
FokI GGATG 2 cut(s) 149, 356
FriOI GRGCYC 1 cut(s) 373
Fsp4HI GCNGC 6 cut(s) 202, 205, 239, 301, 328, 331
FspBI CTAG 1 cut(s) 339
GlaI GCGC 1 cut(s) 299
GluI GCNGC 6 cut(s) 202, 205, 239, 301, 328, 331
GsaI CCCAGC 1 cut(s) 361
GsuI CTGGAG 1 cut(s) 249
HaeIII GGCC 2 cut(s) 159, 207
HapII CCGG 2 cut(s) 134, 160
HhaI GCGC 1 cut(s) 300
Hin1II CATG 1 cut(s) 311
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HinfI GANTC 3 cut(s) 174, 252, 264
HpaII CCGG 2 cut(s) 134, 160
HphI GGTGA 2 cut(s) 91, 100
Hpy188I TCNGA 5 cut(s) 46, 70, 75, 117, 281
HpyAV CCTTC 1 cut(s) 79
HpyCH4III ACNGT 2 cut(s) 195, 221
HpyCH4V TGCA 2 cut(s) 307, 317
HpyF10VI GCNNNNNNNGC 2 cut(s) 213, 304
HpyF3I CTNAG 3 cut(s) 43, 247, 294
Hsp92II CATG 1 cut(s) 311
HspAI GCGC 1 cut(s) 298
Kzo9I GATC 2 cut(s) 61, 70
LmnI GCTCC 4 cut(s) 18, 170, 368, 376
LpnPI CCDG 7 cut(s) 43, 147, 163, 173, 213, 303, 371
Lsp1109I GCAGC 1 cut(s) 312
MaeI CTAG 1 cut(s) 339
MaeIII GTNAC 1 cut(s) 143
MalI GATC 2 cut(s) 63, 72
MbiI CCGCTC 1 cut(s) 21
MboI GATC 2 cut(s) 61, 70
MboII GAAGA 2 cut(s) 30, 133
MfeI CAATTG 1 cut(s) 258
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 3 cut(s) 133, 167, 373
MluCI AATT 2 cut(s) 106, 258
MlyI GAGTC 1 cut(s) 261
MmeI TCCRAC 1 cut(s) 357
MnlI CCTC 4 cut(s) 52, 75, 218, 303
MslI CAYNNNNRTG 1 cut(s) 312
MspA1I CMGCKG 3 cut(s) 238, 327, 361
MspI CCGG 2 cut(s) 134, 160
MspR9I CCNGG 1 cut(s) 134
MunI CAATTG 1 cut(s) 258
MwoI GCNNNNNNNGC 2 cut(s) 213, 304
NciI CCSGG 1 cut(s) 134
NdeII GATC 2 cut(s) 61, 70
NlaIII CATG 1 cut(s) 311
PaqCI CACCTGC 1 cut(s) 308
PfeI GAWTC 2 cut(s) 174, 264
Pfl23II CGTACG 1 cut(s) 221
PkrI GCNGC 6 cut(s) 203, 206, 240, 302, 329, 332
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
Psp124BI GAGCTC 1 cut(s) 373
PspFI CCCAGC 1 cut(s) 357
PspLI CGTACG 1 cut(s) 221
PsuI RGATCY 1 cut(s) 70
RsaI GTAC 2 cut(s) 197, 223
RsaNI GTAC 2 cut(s) 196, 222
RseI CAYNNNNRTG 1 cut(s) 312
SacI GAGCTC 1 cut(s) 373
SatI GCNGC 6 cut(s) 202, 205, 239, 301, 328, 331
Sau3AI GATC 2 cut(s) 61, 70
SchI GAGTC 1 cut(s) 261
ScrFI CCNGG 1 cut(s) 134
SduI GDGCHC 3 cut(s) 133, 167, 373
SetI ASST 6 cut(s) 90, 218, 295, 305, 322, 373
SmiMI CAYNNNNRTG 1 cut(s) 312
Sse9I AATT 2 cut(s) 106, 258
SspMI CTAG 1 cut(s) 339
SstI GAGCTC 1 cut(s) 373
StyD4I CCNGG 1 cut(s) 132
StyI CCWWGG 1 cut(s) 169
TaaI ACNGT 2 cut(s) 195, 221
TaqI TCGA 1 cut(s) 104
TasI AATT 2 cut(s) 106, 258
TauI GCSGC 5 cut(s) 204, 207, 241, 330, 333
TfiI GAWTC 2 cut(s) 174, 264
TscAI CASTG 1 cut(s) 160
TseI GCWGC 1 cut(s) 300
TspDTI ATGAA 1 cut(s) 134
TspGWI ACGGA 2 cut(s) 20, 363
TspRI CASTG 1 cut(s) 160
XspI CTAG 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.