Rh2DG564200

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0005671
Physical Location & Seq
Forward (+)
79341408 .. 79346788
5381 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG564200.1

Sequence Viewer

Length: 2727 bp
ATGGAGAAGAGAGAAAAGGTGAAGTTGATAGTAGCGGCGGCCCTCGCTTCTCTCTCCGTCTTCTTCCTCTACCGGAGGAGACGCCGAAACTGCCCCCAAACTCACTGTTATTTACGAGACGACCCGAACAAGCCGCAGCACGGATTCAAGCGAGTCTTGGCCGACAATTCCTACTCTCCGTTCAAGCATTTCAACCCTGCCGACGACTCTAACGTCGGCGGAGCAGATAAGAATTGGAATTCGCATCCTTATGAGGCGGAGATCACGGCGGTGATGGAAAGTCCGCCGATTTGTGTGACGGAGAATTTGGATTCGGAAATGAGGGAGGAGTATGTTTGGGTTGAGAATGAGACTCAGTTGAAGGAAATGGTTGAGGTTTTGAGTAAAGAGAAGATCTTTGGGGTTGATACTGAGCAGCATAGCTTAAGGTCATTTTTGGGATTTACTGCTTTGATACAGATTTCGACTGAGAAGGAAGATTATTTGGTGGACACTATTGCTTTACATGACAGTATGAGCCTTTTGCAGCCGGTTTTCGCCGATGCTGGTATCTGCAAGGTGTTCCATGGGGCAGATAGTGATGTTCTCTGGCTGCAAAGGGACTTTCATATTTATGTTGTCAATATGTTTGATACTGCAAAGGCTTGTGAAGTGTTGTCAAAACCGCAGAAATCACTGGCTTATTTACTTGAATCTTACTGTGGAGTCGTCACTAACAAATTGTTTCAGCGTGAAGACTGGAGACAACGCCCTTTGTCAATGGAAATGGTGCAATATGCTCGGACAGATGCACACTATCTACTTTACATTGCAGGCCGTTTACTAGCTGAGCTGGATGTGTTGGATAATGAAAACTCTTGTCCCAATGACAAATTCCGTTTTGTTCTCGAGGCTAGTCGGCGTTCAAACATGATATGTTTGCAACTGTATACAAAAGACATAGAAGCTTCGCCTGGAGAGTCTGCTGCTTCATCAATATTTAGCCGTCAATTAAATGGCCAAGGAGGCATTTCATCAGCATCTTATGAAATCCAGAATTCTGTGAGACGACTTTGTGCATGGAGAGATTTGATGGCTCGTGTACATGATGAGAGCTTGAGATATGTAATATCAGATCAGGCTATTGTTGCCCTTGCAGAAAAAGCTCCAACCAACCCTGCAGACATGTTAACCACCATTGCAGAAGCTGATGATAATGTGGATTCAAGTTTCAATTCTGGCCTCTCATCTCCCTCTCCTGTGGTCTGCAGTCATTTGAATGATTTTTATTATCTGCTCCAGAAGAAAGTAGGCAATCCCGATGATCTCTTACCCATGATTCTTCAGAATTGCCTGGGTCAAAAAGGGAGTTGCCCACTGTCTGTCTTCAACTATGCTTTACTGGTTAATTATAATATGAAAGTACCACTGGTTTCTAAACAAAATGGGGTCAGAAATCCAAAGCAAGTCGGTCGAATGGCTTCGCGAAAGCTGTTTGTTCAAAAATTTTCCTGCAAATCCCCAGTTTATCATAATTGCAGGATCTATGCAAATGATGGGCGTTTGCTGTGTTACTGTGACCGCAGAAAGCTTGAATGGTATCTTCATCGGAATTTGGCTAAACTTGTTGAGGACAATCCACCTGGAATTATGCTTCTTTTTGAGCCCAAAGGCCGTCCAGAAGATGAAGGCAATGATTTCTACATTCAAAGTAAGAAAAATATGTGTGTTGGCTGCGGTGAAAGAAATCATTATTTACGGTACCGAATTATTCCCTCATGCTACAGAATGCACTTCCCTGAACATTTGAAAAGCCACCGTTCTCATGATATTGTCCTACTATGCGTGGACTGTCACGAAATTGCTCATGCCGCTGCAGAGAAGTATAAAAGAAAGATTGCTGCAGAATTTGGGATTCCACTTTTTGTTCGTAAAGTGGTTGATTCACAACAAGCTCAATCTTTATCTGGGGCATCTGTGCCTGCAACAAATGTTGAGGAAGCAGGTGTATCTCCTTTACAGTTGCGAACTGCTGCTATGGCTCTTTTGCGCCATGCATCAACAATGCCATCCAAGCGCCGTGAGGAACTGACAGAGATTGTTATGACATTTTATGGAGGGAGGCAAATCTCTGAGGAAGATTTAGAAAAGGCTTTGCTTGTTGGGATGACTCCTCATGAGAAAAGACGACTTCAGCAGAAGAAAGGGTTTTCTTTTCAACATTCTAGAGGGAGTATTTCTTCAGATGCAGAGGAGAAAATTACTGTTAACATGTCCACATCTGCCACTCCAGATGCTTCAGAAGTTGATACTGTACATGAATCTTGCACCAATGAAGCCAAAGCATCTATGATAAACCAAGATGAAGATGTTTTCACAGTGAAGGATGCTGATTTAGGCAACTCCGCTTTAGATTTGAGGATTGATGAAAAAGCATCTCAAAGCAATGAGAAGAACATGAGTTCCGATACTACACTTGTTTCTGTTGTAAATGGTGACGGTATTTATGACAGTAGAAGTACACCAAATGGATCAGTACACTTCCGTTCTACTTCCAAACGTAATCATAAATCAAAACACTCCCTCTTAGGACATGGTCCACATGGAAGGCAAGTTGTGGATCATCTGTTAAAGGAATCTGGAGAAGATGGTATTAGTCAGTTCTGTCAAAGGTGGAGACAAGTATTTGTTGAAACTGTTCATCCCCGTTTTTTACCTGGCGGCTGGGATATTACACACAGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0000178 GO:0000460 GO:0000785 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006403 GO:0006725 GO:0006807 GO:0007549 GO:0008150 GO:0008152 GO:0008298 GO:0008334 GO:0008408 GO:0009048 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010639 GO:0016070 GO:0016071 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032210 GO:0032211 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035327 GO:0040029 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045934 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051179 GO:0051641 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071025 GO:0071027 GO:0071028 GO:0071029 GO:0071030 GO:0071033 GO:0071034 GO:0071035 GO:0071043 GO:0071044 GO:0071046 GO:0071048 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902494 GO:1904356 GO:1904357 GO:1904872 GO:1905354 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1 HRDC

Protein Analysis

908

Amino Acids

102.71

Weight (kDa)

6.63

Isoelectric Point (pI)

50.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 111 - 278 2.6e-50 3'-5' exonuclease
HRDC PF00570 348 - 391 1.2e-09 HRDC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1392
AarI CACCTGC 1 cut(s) 1973
AasI GACNNNNNNGTC 1 cut(s) 212
Acc36I ACCTGC 1 cut(s) 1973
Acc65I GGTACC 1 cut(s) 1740
AccB1I GGYRCC 1 cut(s) 1740
AccI GTMKAC 1 cut(s) 929
AccII CGCG 1 cut(s) 1465
AclWI GGATC 3 cut(s) 1529, 2518, 2607
AcoI YGGCCR 2 cut(s) 159, 997
AcsI RAATTY 7 cut(s) 238, 304, 872, 1036, 1484, 1591, 1886
AcuI CTGAAG 4 cut(s) 1307, 2156, 2205, 2262
AcyI GRCGYC 1 cut(s) 82
AfaI GTAC 6 cut(s) 1083, 1404, 1742, 2295, 2500, 2517
AfiI CCNNNNNNNGG 1 cut(s) 140
AflII CTTAAG 1 cut(s) 424
AflIII ACRYGT 2 cut(s) 1164, 2250
AjnI CCWGG 4 cut(s) 952, 1332, 1621, 2695
AjuI GAANNNNNNNTTGG 2 cut(s) 857, 889
AleI CACNNNNGTG 1 cut(s) 269
AloI GAACNNNNNNTCC 2 cut(s) 2426, 2458
Alw26I GTCTC 6 cut(s) 73, 111, 344, 736, 1039, 2650
AlwI GGATC 3 cut(s) 1529, 2518, 2607
AlwNI CAGNNNCTG 1 cut(s) 1187
Ama87I CYCGRG 1 cut(s) 887
AoxI GGCC 6 cut(s) 39, 159, 814, 997, 1219, 1651
ApeKI GCWGC 9 cut(s) 136, 415, 526, 592, 965, 1713, 1853, 1880, 2012
ApoI RAATTY 7 cut(s) 238, 304, 872, 1036, 1484, 1591, 1886
ArsI GACNNNNNNTTYG 2 cut(s) 289, 321
Asp700I GAANNNNTTC 2 cut(s) 1459, 2676
Asp718I GGTACC 1 cut(s) 1740
AspLEI GCGC 2 cut(s) 2031, 2058
AspS9I GGNCC 2 cut(s) 40, 2576
AsuHPI GGTGA 4 cut(s) 31, 283, 1730, 2486
AvaI CYCGRG 1 cut(s) 887
AvaII GGWCC 1 cut(s) 2576
BalI TGGCCA 1 cut(s) 999
BanI GGYRCC 1 cut(s) 1740
BanII GRGCYC 1 cut(s) 1647
BauI CACGAG 1 cut(s) 1077
BbsI GAAGAC 3 cut(s) 52, 741, 1357
BbvI GCAGC 9 cut(s) 148, 427, 538, 579, 952, 1700, 1840, 1867, 1999
BccI CCATC 5 cut(s) 268, 1066, 1529, 2056, 2621
BceAI ACGGC 5 cut(s) 282, 801, 969, 1638, 2043
BcgI CGANNNNNNTGC 4 cut(s) 761, 795, 1433, 1467
BciT130I CCWGG 4 cut(s) 954, 1334, 1623, 2697
BcoDI GTCTC 6 cut(s) 73, 111, 344, 736, 1039, 2650
BfaI CTAG 3 cut(s) 824, 894, 2205
BfmI CTRYAG 5 cut(s) 1158, 1246, 1762, 1854, 1881
BfoI RGCGCY 1 cut(s) 2059
BfrI CTTAAG 1 cut(s) 424
BfuAI ACCTGC 1 cut(s) 1973
BglI GCCNNNNNGGC 1 cut(s) 1005
BglII AGATCT 1 cut(s) 393
BlpI GCTNAGC 1 cut(s) 828
Bme1390I CCNGG 4 cut(s) 954, 1334, 1623, 2697
Bme18I GGWCC 1 cut(s) 2576
BmeT110I CYCGRG 1 cut(s) 887
BmgT120I GGNCC 2 cut(s) 40, 2576
BmiI GGNNCC 1 cut(s) 1742
BmrFI CCNGG 4 cut(s) 954, 1334, 1623, 2697
BmrI ACTGGG 1 cut(s) 1496
BmuI ACTGGG 1 cut(s) 1496
BpiI GAAGAC 3 cut(s) 52, 741, 1357
BpmI CTGGAG 5 cut(s) 760, 975, 1262, 2253, 2640
Bpu1102I GCTNAGC 1 cut(s) 828
BpuEI CTTGAG 1 cut(s) 1117
BsaHI GRCGYC 1 cut(s) 82
BsaJI CCNNGG 3 cut(s) 565, 1000, 1333
BsaWI WCCGGW 1 cut(s) 72
Bsc4I CCNNNNNNNGG 1 cut(s) 140
Bse118I RCCGGY 1 cut(s) 529
Bse1I ACTGG 5 cut(s) 681, 743, 1386, 1413, 1502
Bse3DI GCAATG 4 cut(s) 807, 1176, 1678, 2431
BseBI CCWGG 4 cut(s) 954, 1334, 1623, 2697
BseDI CCNNGG 3 cut(s) 565, 1000, 1333
BseGI GGATG 6 cut(s) 244, 841, 2048, 2151, 2371, 2680
BseLI CCNNNNNNNGG 1 cut(s) 140
BseMI GCAATG 4 cut(s) 807, 1176, 1678, 2431
BseMII CTCAG 5 cut(s) 368, 402, 459, 819, 2103
BseNI ACTGG 5 cut(s) 681, 743, 1386, 1413, 1502
BseRI GAGGAG 4 cut(s) 91, 341, 2142, 2246
BseXI GCAGC 9 cut(s) 148, 427, 538, 579, 952, 1700, 1840, 1867, 1999
BseYI CCCAGC 1 cut(s) 2703
Bsh1236I CGCG 1 cut(s) 1465
Bsh1285I CGRYCG 1 cut(s) 1453
BshFI GGCC 6 cut(s) 41, 161, 816, 999, 1221, 1653
BshNI GGYRCC 1 cut(s) 1740
BsiEI CGRYCG 1 cut(s) 1453
BsiHKCI CYCGRG 1 cut(s) 887
BsiSI CCGG 2 cut(s) 73, 530
BslFI GGGAC 2 cut(s) 614, 846
BslI CCNNNNNNNGG 1 cut(s) 140
BsmAI GTCTC 6 cut(s) 73, 111, 344, 736, 1039, 2650
BsmBI CGTCTC 3 cut(s) 73, 111, 1039
BsmFI GGGAC 2 cut(s) 614, 846
BsmI GAATGC 1 cut(s) 1773
BsnI GGCC 6 cut(s) 41, 161, 816, 999, 1221, 1653
BsoBI CYCGRG 1 cut(s) 887
Bsp1286I GDGCHC 1 cut(s) 1647
Bsp1407I TGTACA 2 cut(s) 1081, 2293
Bsp143I GATC 7 cut(s) 261, 393, 1114, 1303, 1521, 2510, 2599
Bsp1720I GCTNAGC 1 cut(s) 828
Bsp19I CCATGG 1 cut(s) 565
Bsp68I TCGCGA 1 cut(s) 1465
BspANI GGCC 6 cut(s) 41, 161, 816, 999, 1221, 1653
BspCNI CTCAG 5 cut(s) 367, 403, 460, 820, 2104
BspFNI CGCG 1 cut(s) 1465
BspHI TCATGA 2 cut(s) 1804, 2155
BspLI GGNNCC 1 cut(s) 1742
BspMAI CTGCAG 4 cut(s) 1162, 1250, 1858, 1885
BspMI ACCTGC 1 cut(s) 1973
BspPI GGATC 3 cut(s) 1529, 2518, 2607
BspT107I GGYRCC 1 cut(s) 1740
BspTI CTTAAG 1 cut(s) 424
BsrDI GCAATG 4 cut(s) 807, 1176, 1678, 2431
BsrFI RCCGGY 1 cut(s) 529
BsrGI TGTACA 2 cut(s) 1081, 2293
BsrI ACTGG 5 cut(s) 681, 743, 1386, 1413, 1502
BssAI RCCGGY 1 cut(s) 529
BssECI CCNNGG 3 cut(s) 565, 1000, 1333
BssMI GATC 7 cut(s) 261, 393, 1114, 1303, 1521, 2510, 2599
BssNAI GTATAC 1 cut(s) 930
BssNI GRCGYC 1 cut(s) 82
BssSI CACGAG 1 cut(s) 1077
BssT1I CCWWGG 2 cut(s) 565, 1000
Bst1107I GTATAC 1 cut(s) 930
Bst2BI CACGAG 1 cut(s) 1077
Bst2UI CCWGG 4 cut(s) 954, 1334, 1623, 2697
Bst6I CTCTTC 1 cut(s) 2
BstACI GRCGYC 1 cut(s) 82
BstAFI CTTAAG 1 cut(s) 424
BstAUI TGTACA 2 cut(s) 1081, 2293
BstC8I GCNNGC 2 cut(s) 814, 1962
BstDEI CTNAG 6 cut(s) 354, 411, 468, 828, 2112, 2566
BstDSI CCRYGG 1 cut(s) 565
BstF5I GGATG 6 cut(s) 244, 841, 2048, 2151, 2371, 2680
BstFNI CGCG 1 cut(s) 1465
BstH2I RGCGCY 1 cut(s) 2059
BstHHI GCGC 2 cut(s) 2031, 2058
BstKTI GATC 7 cut(s) 264, 396, 1117, 1306, 1524, 2513, 2602
BstMAI GTCTC 6 cut(s) 73, 111, 344, 736, 1039, 2650
BstMBI GATC 7 cut(s) 261, 393, 1114, 1303, 1521, 2510, 2599
BstMCI CGRYCG 1 cut(s) 1453
BstMWI GCNNNNNNNGC 8 cut(s) 44, 90, 1005, 1127, 1142, 1850, 2018, 2053
BstNI CCWGG 4 cut(s) 954, 1334, 1623, 2697
BstNSI RCATGY 2 cut(s) 1168, 2254
BstSCI CCNGG 4 cut(s) 952, 1332, 1621, 2695
BstSFI CTRYAG 5 cut(s) 1158, 1246, 1762, 1854, 1881
BstUI CGCG 1 cut(s) 1465
BstV1I GCAGC 9 cut(s) 148, 427, 538, 579, 952, 1700, 1840, 1867, 1999
BstV2I GAAGAC 3 cut(s) 52, 741, 1357
BstX2I RGATCY 2 cut(s) 393, 1521
BstYI RGATCY 2 cut(s) 393, 1521
BstZ17I GTATAC 1 cut(s) 930
BsuRI GGCC 6 cut(s) 41, 161, 816, 999, 1221, 1653
BtgI CCRYGG 1 cut(s) 565
BtsCI GGATG 6 cut(s) 244, 841, 2048, 2151, 2371, 2680
BtsIMutI CAGTG 5 cut(s) 103, 674, 1355, 1406, 2364
BtuMI TCGCGA 1 cut(s) 1465
BveI ACCTGC 1 cut(s) 1973
Cac8I GCNNGC 2 cut(s) 814, 1962
CaiI CAGNNNCTG 1 cut(s) 1187
CciI TCATGA 2 cut(s) 1804, 2155
CfoI GCGC 2 cut(s) 2031, 2058
Cfr10I RCCGGY 1 cut(s) 529
Cfr13I GGNCC 2 cut(s) 40, 2576
CseI GACGC 1 cut(s) 90
Csp6I GTAC 6 cut(s) 1082, 1403, 1741, 2294, 2499, 2516
CviQI GTAC 6 cut(s) 1082, 1403, 1741, 2294, 2499, 2516
DdeI CTNAG 6 cut(s) 354, 411, 468, 828, 2112, 2566
DpnI GATC 7 cut(s) 263, 395, 1116, 1305, 1523, 2512, 2601
DpnII GATC 7 cut(s) 261, 393, 1114, 1303, 1521, 2510, 2599
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
EaeI YGGCCR 2 cut(s) 159, 997
Eam1104I CTCTTC 1 cut(s) 2
EarI CTCTTC 1 cut(s) 2
EciI GGCGGA 3 cut(s) 234, 272, 273
Eco130I CCWWGG 2 cut(s) 565, 1000
Eco24I GRGCYC 1 cut(s) 1647
Eco47I GGWCC 1 cut(s) 2576
Eco57I CTGAAG 4 cut(s) 1307, 2156, 2205, 2262
Eco88I CYCGRG 1 cut(s) 887
EcoRI GAATTC 2 cut(s) 238, 1036
EcoRII CCWGG 4 cut(s) 952, 1332, 1621, 2695
EcoT14I CCWWGG 2 cut(s) 565, 1000
EcoT22I ATGCAT 1 cut(s) 2038
EcoT38I GRGCYC 1 cut(s) 1647
ErhI CCWWGG 2 cut(s) 565, 1000
Esp3I CGTCTC 3 cut(s) 73, 111, 1039
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FaqI GGGAC 2 cut(s) 614, 846
FblI GTMKAC 1 cut(s) 929
FokI GGATG 6 cut(s) 231, 848, 2035, 2158, 2378, 2667
FriOI GRGCYC 1 cut(s) 1647
FspBI CTAG 3 cut(s) 824, 894, 2205
GlaI GCGC 2 cut(s) 2030, 2057
GsaI CCCAGC 1 cut(s) 2707
GsuI CTGGAG 5 cut(s) 760, 975, 1262, 2253, 2640
HaeII RGCGCY 1 cut(s) 2059
HaeIII GGCC 6 cut(s) 41, 161, 816, 999, 1221, 1653
HapII CCGG 2 cut(s) 73, 530
HgaI GACGC 1 cut(s) 90
HhaI GCGC 2 cut(s) 2031, 2058
Hin1I GRCGYC 1 cut(s) 82
Hin6I GCGC 2 cut(s) 2029, 2056
HinP1I GCGC 2 cut(s) 2029, 2056
HincII GTYRAC 2 cut(s) 1170, 2248
HindII GTYRAC 2 cut(s) 1170, 2248
HindIII AAGCTT 2 cut(s) 945, 1568
HpaI GTTAAC 2 cut(s) 1170, 2248
HpaII CCGG 2 cut(s) 73, 530
HphI GGTGA 4 cut(s) 31, 283, 1730, 2486
Hpy99I CGWCG 2 cut(s) 206, 218
HpyAV CCTTC 5 cut(s) 355, 466, 1661, 2356, 2580
HpyCH4IV ACGT 2 cut(s) 213, 2539
HpyF10VI GCNNNNNNNGC 8 cut(s) 44, 90, 1005, 1127, 1142, 1850, 2018, 2053
HpyF3I CTNAG 6 cut(s) 354, 411, 468, 828, 2112, 2566
HpySE526I ACGT 2 cut(s) 213, 2539
Hsp92I GRCGYC 1 cut(s) 82
HspAI GCGC 2 cut(s) 2029, 2056
KpnI GGTACC 1 cut(s) 1744
KspAI GTTAAC 2 cut(s) 1170, 2248
Kzo9I GATC 7 cut(s) 261, 393, 1114, 1303, 1521, 2510, 2599
LmnI GCTCC 3 cut(s) 221, 1150, 1281
Lsp1109I GCAGC 9 cut(s) 148, 427, 538, 579, 952, 1700, 1840, 1867, 1999
MaeI CTAG 3 cut(s) 824, 894, 2205
MaeII ACGT 2 cut(s) 213, 2539
MaeIII GTNAC 6 cut(s) 295, 709, 1550, 1556, 1832, 2474
MalI GATC 7 cut(s) 263, 395, 1116, 1305, 1523, 2512, 2601
MboI GATC 7 cut(s) 261, 393, 1114, 1303, 1521, 2510, 2599
MflI RGATCY 2 cut(s) 393, 1521
MhlI GDGCHC 1 cut(s) 1647
MlsI TGGCCA 1 cut(s) 999
MluNI TGGCCA 1 cut(s) 999
MlyI GAGTC 6 cut(s) 162, 200, 346, 714, 968, 2143
MmeI TCCRAC 2 cut(s) 822, 1172
Mox20I TGGCCA 1 cut(s) 999
Mph1103I ATGCAT 1 cut(s) 2038
MroXI GAANNNNTTC 2 cut(s) 1459, 2676
MscI TGGCCA 1 cut(s) 999
MseI TTAA 6 cut(s) 425, 992, 1169, 1386, 2247, 2609
MslI CAYNNNNRTG 4 cut(s) 249, 269, 612, 1257
Msp20I TGGCCA 1 cut(s) 999
MspA1I CMGCKG 1 cut(s) 1853
MspCI CTTAAG 1 cut(s) 424
MspI CCGG 2 cut(s) 73, 530
MspR9I CCNGG 4 cut(s) 954, 1334, 1623, 2697
Mva1269I GAATGC 1 cut(s) 1773
MvaI CCWGG 4 cut(s) 954, 1334, 1623, 2697
MvnI CGCG 1 cut(s) 1465
MwoI GCNNNNNNNGC 8 cut(s) 44, 90, 1005, 1127, 1142, 1850, 2018, 2053
NcoI CCATGG 1 cut(s) 565
NdeII GATC 7 cut(s) 261, 393, 1114, 1303, 1521, 2510, 2599
NlaIV GGNNCC 1 cut(s) 1742
NmuCI GTSAC 5 cut(s) 295, 709, 1556, 1832, 2474
NruI TCGCGA 1 cut(s) 1465
NsiI ATGCAT 1 cut(s) 2038
NspI RCATGY 2 cut(s) 1168, 2254
OliI CACNNNNGTG 1 cut(s) 269
PaeR7I CTCGAG 1 cut(s) 887
PagI TCATGA 2 cut(s) 1804, 2155
PaqCI CACCTGC 1 cut(s) 1973
PciI ACATGT 2 cut(s) 1164, 2250
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PctI GAATGC 1 cut(s) 1773
PdmI GAANNNNTTC 2 cut(s) 1459, 2676
PfeI GAWTC 9 cut(s) 144, 311, 692, 1202, 1318, 1894, 1922, 2300, 2615
PflFI GACNNNGTC 1 cut(s) 2574
PleI GAGTC 6 cut(s) 161, 200, 346, 713, 967, 2143
PpsI GAGTC 6 cut(s) 161, 200, 346, 713, 967, 2143
PscI ACATGT 2 cut(s) 1164, 2250
PsiI TTATAA 1 cut(s) 1392
Psp6I CCWGG 4 cut(s) 952, 1332, 1621, 2695
PspFI CCCAGC 1 cut(s) 2703
PspGI CCWGG 4 cut(s) 952, 1332, 1621, 2695
PspN4I GGNNCC 1 cut(s) 1742
PspPI GGNCC 2 cut(s) 40, 2576
PstI CTGCAG 4 cut(s) 1162, 1250, 1858, 1885
PstNI CAGNNNCTG 1 cut(s) 1187
PsuI RGATCY 2 cut(s) 393, 1521
PsyI GACNNNGTC 1 cut(s) 2574
RruI TCGCGA 1 cut(s) 1465
RsaI GTAC 6 cut(s) 1083, 1404, 1742, 2295, 2500, 2517
RsaNI GTAC 6 cut(s) 1082, 1403, 1741, 2294, 2499, 2516
RseI CAYNNNNRTG 4 cut(s) 249, 269, 612, 1257
SaqAI TTAA 6 cut(s) 425, 992, 1169, 1386, 2247, 2609
Sau3AI GATC 7 cut(s) 261, 393, 1114, 1303, 1521, 2510, 2599
Sau96I GGNCC 2 cut(s) 40, 2576
SchI GAGTC 6 cut(s) 162, 200, 346, 714, 968, 2143
ScrFI CCNGG 4 cut(s) 954, 1334, 1623, 2697
SduI GDGCHC 1 cut(s) 1647
SfcI CTRYAG 5 cut(s) 1158, 1246, 1762, 1854, 1881
Sfr274I CTCGAG 1 cut(s) 887
SinI GGWCC 1 cut(s) 2576
SlaI CTCGAG 1 cut(s) 887
SmiMI CAYNNNNRTG 4 cut(s) 249, 269, 612, 1257
SmlI CTYRAG 3 cut(s) 424, 887, 1096
SmoI CTYRAG 3 cut(s) 424, 887, 1096
SspI AATATT 1 cut(s) 978
SspMI CTAG 3 cut(s) 824, 894, 2205
StyD4I CCNGG 4 cut(s) 952, 1332, 1621, 2695
StyI CCWWGG 2 cut(s) 565, 1000
TaiI ACGT 2 cut(s) 216, 2542
TaqI TCGA 3 cut(s) 464, 888, 1453
TaqII GACCGA 1 cut(s) 1439
TatI WGTACW 4 cut(s) 1081, 2293, 2498, 2515
TauI GCSGC 5 cut(s) 38, 41, 136, 1853, 2703
TfiI GAWTC 9 cut(s) 144, 311, 692, 1202, 1318, 1894, 1922, 2300, 2615
Tru1I TTAA 6 cut(s) 425, 992, 1169, 1386, 2247, 2609
Tru9I TTAA 6 cut(s) 425, 992, 1169, 1386, 2247, 2609
TscAI CASTG 5 cut(s) 110, 681, 1362, 1413, 2364
TseFI GTSAC 5 cut(s) 295, 709, 1556, 1832, 2474
TseI GCWGC 9 cut(s) 136, 415, 526, 592, 965, 1713, 1853, 1880, 2012
Tsp45I GTSAC 5 cut(s) 295, 709, 1556, 1832, 2474
TspGWI ACGGA 6 cut(s) 46, 156, 168, 314, 866, 2513
TspRI CASTG 5 cut(s) 110, 681, 1362, 1413, 2364
Tth111I GACNNNGTC 1 cut(s) 2574
Vha464I CTTAAG 1 cut(s) 424
VpaK11BI GGWCC 1 cut(s) 2576
XapI RAATTY 7 cut(s) 238, 304, 872, 1036, 1484, 1591, 1886
XbaI TCTAGA 1 cut(s) 2204
XceI RCATGY 2 cut(s) 1168, 2254
XhoI CTCGAG 1 cut(s) 887
XmiI GTMKAC 1 cut(s) 929
XmnI GAANNNNTTC 2 cut(s) 1459, 2676
XspI CTAG 3 cut(s) 824, 894, 2205
Zsp2I ATGCAT 1 cut(s) 2038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.