Rorug02G0475300

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005671
Physical Location & Seq
Reverse (-)
60648661 .. 60650532
1872 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0475300.1

Sequence Viewer

Length: 1515 bp
ATGGCCGCCGCAATCACCCCAGCTCCTTCGTCCTCCACCCCAACACCCACCAAAGTGTCCCCCAAGACGGCCCGAAATGCCGTGAATGCCCTTCTGCAATGGCGAAATTCCAAGTCGGAGGCCCAGAAACCCGACCTCCTCTCCTCCGACGAACTACTCTACCTCGTCGTGAGCCTGAAGAAGATCCCACCCAAGGGCCGCGTCAACGCGTACAAAATCCCCCTCCCGACTCCCCTCCACTCCCAGCTCACCGAGTTCTGCCTCATCTACGACGACCGGCCCAAGTCCAAGCTCACCAAGGCCCAAATTCAGGCCAAGATCAAGGCCGACAACTTGCCCGTAGTCAAAATCCTGAAGTACACCAAGCTGAAATCAGATTACAAGGCTTTCGAGTCGAAAAGGAAGCTGCTCAACTCGTATGATGTGTTTCTTGCTGATAAGCAGATCGTGCCATTGCTGCCGAGGCTGATCGGGAAGCAGTTCTTCAAGAAGAAGAAGATTCCGGTGCCGGTGGACTTGTTGCACAAGAATTGGAAGGAGCAGATTGATCGGATATGCGGCTCGGCGTTGTTGTTCTTGACTACTGGGACTTGCAGTGTGGTGAGGGTGGCGAGGACTTCGATGAGGGAGGAGGAGATTGTGGAGAATGTGGTTTCGGCGATTAGTGGGATTGTGGAGATTGTGCCCGGGAGTTGGGGCGGTGTGAGGTCGTTGCATTTGAAGCTATTGGAGTCGATTCCGCTGCCGCTTTACCAGACTCTGCCGGATGAGGCGGTGAAGGTGGAGGGGGCTGAGAAAGTTGTGGAGGCTGGGAAGGGTGAGAGTAAGGAATTGAAGAAGGAGAAGGTGGGGAAGAAGAAGGGGATGATTCATGAAGTGAGGTATTTGGATAGCAGTGCTGGTGAGGTCGTTGATGAGGTGAAATCAGGTAAGGATAGTGATGTGGATGAGCAACTGGGTGGTGGTGAGTTGAAGAAGGCGAAGAGGAAGAAGGACAAGGTTGTTGGTGAGTCCAATGGTAAAGCTAGTGAGAATGATGGTGATGGTGAGTTGAGGAAGTCGAAGAGGAACAAGGAGAAGGTTGTTGGTGAGTCTAAGAGGTCAAAGGAACAGATGGAGAAGTTAGCTAAGCTGGTAGATGAAGATGGTGATTTTGATGTGGAAGTTGCTGAGAAGCGTACGAAGAAGGTGGTTAATCCTAAGTCGGGTAAGGTGAAAGGTGAAGATGTTGAGAAACAGTTGAAAAAGTCGGCTAAGGCGAAAGATGTTGCTTTGAATGGGGAGAAACCATTGAAGAAGTCGGGTAAGGTGAAAGATGATGCTGGCACCATTGTCAAGCATAAAAATGATGAGTTGTCTGCGAAAGACAAGAAAAAAGATGTTATAAAGAAGAAAGGAGATGGGTTGTCTGGCAAAGGCGGACAGGCTGTAGGAAAGAAGGAGAAGAGGAAGAGTGAGCCTGTGAAGTTGAAGAGTGAAGAGGCAAAGCTCAAGAAAGCCAAGAGAAGTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0000178 GO:0000460 GO:0000785 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006403 GO:0006725 GO:0006807 GO:0007549 GO:0008150 GO:0008152 GO:0008298 GO:0008334 GO:0008408 GO:0009048 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010639 GO:0016070 GO:0016071 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032210 GO:0032211 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035327 GO:0040029 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045934 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051179 GO:0051641 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071025 GO:0071027 GO:0071028 GO:0071029 GO:0071030 GO:0071033 GO:0071034 GO:0071035 GO:0071043 GO:0071044 GO:0071046 GO:0071048 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902494 GO:1904356 GO:1904357 GO:1904872 GO:1905354 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1 HRDC

Protein Analysis

504

Amino Acids

55.57

Weight (kDa)

9.75

Isoelectric Point (pI)

26.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L1 PF00687 49 - 245 5.9e-44 Ribosomal protein L1p/L10e family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1385
AccB1I GGYRCC 2 cut(s) 505, 1325
AccII CGCG 2 cut(s) 201, 209
AciI CCGC 9 cut(s) 6, 9, 199, 558, 699, 740, 746, 773, 1419
AclWI GGATC 1 cut(s) 178
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 106, 306
AcuI CTGAAG 2 cut(s) 197, 374
AfaI GTAC 3 cut(s) 212, 359, 1180
AfiI CCNNNNNNNGG 6 cut(s) 67, 193, 194, 310, 693, 1205
AflIII ACRYGT 1 cut(s) 207
AgsI TTSAA 8 cut(s) 487, 721, 835, 973, 1243, 1276, 1294, 1471
AleI CACNNNNGTG 1 cut(s) 53
AlwI GGATC 1 cut(s) 178
AlwNI CAGNNNCTG 1 cut(s) 760
Ama87I CYCGRG 1 cut(s) 686
AoxI GGCC 8 cut(s) 3, 69, 120, 196, 278, 300, 312, 324
ApeKI GCWGC 3 cut(s) 406, 457, 742
ApoI RAATTY 2 cut(s) 106, 306
Asp700I GAANNNNTTC 1 cut(s) 479
AspS9I GGNCC 5 cut(s) 70, 121, 196, 279, 301
AsuC2I CCSGG 2 cut(s) 687, 688
AvaI CYCGRG 1 cut(s) 686
BaeGI GKGCMC 1 cut(s) 687
BanI GGYRCC 2 cut(s) 505, 1325
BbvI GCAGC 3 cut(s) 393, 444, 729
BccI CCATC 5 cut(s) 1031, 1037, 1108, 1139, 1394
BceAI ACGGC 2 cut(s) 65, 84
BcgI CGANNNNNNTGC 4 cut(s) 530, 564, 724, 758
BcnI CCSGG 2 cut(s) 687, 688
BfaI CTAG 1 cut(s) 1026
BfmI CTRYAG 1 cut(s) 1428
BisI GCNGC 8 cut(s) 6, 9, 199, 407, 458, 559, 743, 746
BlpI GCTNAGC 1 cut(s) 1128
BlsI GCNGC 8 cut(s) 7, 10, 200, 408, 459, 560, 744, 747
Bme1390I CCNGG 2 cut(s) 687, 688
BmeT110I CYCGRG 1 cut(s) 686
BmgT120I GGNCC 5 cut(s) 70, 121, 196, 279, 301
BmiI GGNNCC 2 cut(s) 507, 1327
BmrFI CCNGG 2 cut(s) 687, 688
BmrI ACTGGG 2 cut(s) 594, 965
BmsI GCATC 1 cut(s) 1309
BmuI ACTGGG 2 cut(s) 594, 965
Bpu10I CCTNAGC 1 cut(s) 1254
Bpu1102I GCTNAGC 1 cut(s) 1128
BpuEI CTTGAG 1 cut(s) 1475
BpuMI CCSGG 2 cut(s) 687, 688
BsaJI CCNNGG 4 cut(s) 192, 297, 461, 686
BsaWI WCCGGW 1 cut(s) 502
BsaXI ACNNNNNCTCC 6 cut(s) 7, 37, 120, 125, 150, 155
Bsc4I CCNNNNNNNGG 6 cut(s) 67, 193, 194, 310, 693, 1205
Bse118I RCCGGY 2 cut(s) 276, 508
Bse1I ACTGG 2 cut(s) 589, 960
Bse3DI GCAATG 2 cut(s) 104, 452
BseDI CCNNGG 4 cut(s) 192, 297, 461, 686
BseGI GGATG 3 cut(s) 772, 870, 952
BseLI CCNNNNNNNGG 6 cut(s) 67, 193, 194, 310, 693, 1205
BseMI GCAATG 2 cut(s) 104, 452
BseMII CTCAG 2 cut(s) 783, 1161
BseNI ACTGG 2 cut(s) 589, 960
BseRI GAGGAG 4 cut(s) 128, 133, 644, 647
BseSI GKGCMC 1 cut(s) 687
BseXI GCAGC 3 cut(s) 393, 444, 729
BseYI CCCAGC 3 cut(s) 19, 243, 809
Bsh1236I CGCG 2 cut(s) 201, 209
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 8 cut(s) 5, 71, 122, 198, 280, 302, 314, 326
BshNI GGYRCC 2 cut(s) 505, 1325
BsiEI CGRYCG 1 cut(s) 277
BsiHKCI CYCGRG 1 cut(s) 686
BsiSI CCGG 5 cut(s) 277, 503, 509, 687, 764
BsiWI CGTACG 1 cut(s) 1178
BslFI GGGAC 2 cut(s) 43, 601
BslI CCNNNNNNNGG 6 cut(s) 67, 193, 194, 310, 693, 1205
BsmFI GGGAC 2 cut(s) 43, 601
BsmI GAATGC 1 cut(s) 91
BsnI GGCC 8 cut(s) 5, 71, 122, 198, 280, 302, 314, 326
BsoBI CYCGRG 1 cut(s) 686
Bsp1286I GDGCHC 1 cut(s) 687
Bsp143I GATC 5 cut(s) 183, 318, 444, 468, 547
Bsp1720I GCTNAGC 1 cut(s) 1128
BspACI CCGC 9 cut(s) 6, 9, 199, 558, 699, 740, 746, 773, 1419
BspANI GGCC 8 cut(s) 5, 71, 122, 198, 280, 302, 314, 326
BspCNI CTCAG 2 cut(s) 784, 1162
BspFNI CGCG 2 cut(s) 201, 209
BspHI TCATGA 1 cut(s) 871
BspLI GGNNCC 2 cut(s) 507, 1327
BspPI GGATC 1 cut(s) 178
BspT107I GGYRCC 2 cut(s) 505, 1325
BsrDI GCAATG 2 cut(s) 104, 452
BsrFI RCCGGY 2 cut(s) 276, 508
BsrI ACTGG 2 cut(s) 589, 960
BssAI RCCGGY 2 cut(s) 276, 508
BssECI CCNNGG 4 cut(s) 192, 297, 461, 686
BssMI GATC 5 cut(s) 183, 318, 444, 468, 547
BssT1I CCWWGG 2 cut(s) 192, 297
Bst4CI ACNGT 1 cut(s) 1239
Bst6I CTCTTC 6 cut(s) 977, 1058, 1439, 1445, 1466, 1473
BstAPI GCANNNNNTGC 1 cut(s) 448
BstC8I GCNNGC 1 cut(s) 1324
BstDEI CTNAG 6 cut(s) 792, 1095, 1128, 1170, 1200, 1254
BstF5I GGATG 3 cut(s) 772, 870, 952
BstFNI CGCG 2 cut(s) 201, 209
BstKTI GATC 5 cut(s) 186, 321, 447, 471, 550
BstMBI GATC 5 cut(s) 183, 318, 444, 468, 547
BstMCI CGRYCG 1 cut(s) 277
BstMWI GCNNNNNNNGC 6 cut(s) 77, 86, 448, 457, 463, 721
BstSCI CCNGG 2 cut(s) 685, 686
BstSFI CTRYAG 1 cut(s) 1428
BstSLI GKGCMC 1 cut(s) 687
BstUI CGCG 2 cut(s) 201, 209
BstV1I GCAGC 3 cut(s) 393, 444, 729
BstX2I RGATCY 1 cut(s) 183
BstYI RGATCY 1 cut(s) 183
BsuRI GGCC 8 cut(s) 5, 71, 122, 198, 280, 302, 314, 326
BtsCI GGATG 3 cut(s) 772, 870, 952
BtsI GCAGTG 2 cut(s) 601, 901
BtsIMutI CAGTG 2 cut(s) 601, 901
Cac8I GCNNGC 1 cut(s) 1324
CaiI CAGNNNCTG 1 cut(s) 760
CciI TCATGA 1 cut(s) 871
Cfr10I RCCGGY 2 cut(s) 276, 508
Cfr13I GGNCC 5 cut(s) 70, 121, 196, 279, 301
Cfr9I CCCGGG 1 cut(s) 686
CseI GACGC 1 cut(s) 190
Csp6I GTAC 3 cut(s) 211, 358, 1179
CviAII CATG 1 cut(s) 872
CviQI GTAC 3 cut(s) 211, 358, 1179
DdeI CTNAG 6 cut(s) 792, 1095, 1128, 1170, 1200, 1254
DpnI GATC 5 cut(s) 185, 320, 446, 470, 549
DpnII GATC 5 cut(s) 183, 318, 444, 468, 547
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 6 cut(s) 977, 1058, 1439, 1445, 1466, 1473
EarI CTCTTC 6 cut(s) 977, 1058, 1439, 1445, 1466, 1473
EciI GGCGGA 1 cut(s) 1434
Eco130I CCWWGG 2 cut(s) 192, 297
Eco57I CTGAAG 2 cut(s) 197, 374
Eco88I CYCGRG 1 cut(s) 686
EcoT14I CCWWGG 2 cut(s) 192, 297
ErhI CCWWGG 2 cut(s) 192, 297
FaeI CATG 1 cut(s) 875
FaiI YATR 5 cut(s) 420, 556, 873, 1341, 1385
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FaqI GGGAC 2 cut(s) 43, 601
FatI CATG 1 cut(s) 871
Fnu4HI GCNGC 8 cut(s) 6, 9, 199, 407, 458, 559, 743, 746
FokI GGATG 3 cut(s) 779, 877, 959
Fsp4HI GCNGC 8 cut(s) 6, 9, 199, 407, 458, 559, 743, 746
FspBI CTAG 1 cut(s) 1026
GluI GCNGC 8 cut(s) 6, 9, 199, 407, 458, 559, 743, 746
GsaI CCCAGC 3 cut(s) 23, 247, 813
HaeIII GGCC 8 cut(s) 5, 71, 122, 198, 280, 302, 314, 326
HapII CCGG 5 cut(s) 277, 503, 509, 687, 764
HgaI GACGC 1 cut(s) 190
Hin1II CATG 1 cut(s) 875
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HinfI GANTC 9 cut(s) 229, 392, 499, 731, 736, 757, 868, 1010, 1091
HpaII CCGG 5 cut(s) 277, 503, 509, 687, 764
Hpy166II GTNNAC 3 cut(s) 205, 360, 514
Hpy188I TCNGA 4 cut(s) 118, 148, 376, 552
Hpy188III TCNNGA 8 cut(s) 169, 226, 352, 472, 487, 577, 872, 1492
Hpy8I GTNNAC 3 cut(s) 205, 360, 514
Hpy99I CGWCG 3 cut(s) 152, 170, 275
HpyCH4III ACNGT 1 cut(s) 1239
HpyCH4V TGCA 4 cut(s) 97, 523, 594, 715
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 86, 448, 457, 463, 721
HpyF3I CTNAG 6 cut(s) 792, 1095, 1128, 1170, 1200, 1254
Hsp92II CATG 1 cut(s) 875
Kzo9I GATC 5 cut(s) 183, 318, 444, 468, 547
LmnI GCTCC 2 cut(s) 28, 538
Lsp1109I GCAGC 3 cut(s) 393, 444, 729
LweI GCATC 1 cut(s) 1309
MaeI CTAG 1 cut(s) 1026
MalI GATC 5 cut(s) 185, 320, 446, 470, 549
MboI GATC 5 cut(s) 183, 318, 444, 468, 547
MflI RGATCY 1 cut(s) 183
MhlI GDGCHC 1 cut(s) 687
MluCI AATT 4 cut(s) 106, 306, 529, 830
MluI ACGCGT 1 cut(s) 207
MlyI GAGTC 6 cut(s) 223, 401, 740, 751, 1019, 1100
MmeI TCCRAC 2 cut(s) 96, 171
MroXI GAANNNNTTC 1 cut(s) 479
MseI TTAA 1 cut(s) 1194
MslI CAYNNNNRTG 2 cut(s) 53, 1344
MspA1I CMGCKG 1 cut(s) 742
MspI CCGG 5 cut(s) 277, 503, 509, 687, 764
MspR9I CCNGG 2 cut(s) 687, 688
Mva1269I GAATGC 1 cut(s) 91
MvnI CGCG 2 cut(s) 201, 209
MwoI GCNNNNNNNGC 6 cut(s) 77, 86, 448, 457, 463, 721
NciI CCSGG 2 cut(s) 687, 688
NdeII GATC 5 cut(s) 183, 318, 444, 468, 547
NlaIII CATG 1 cut(s) 875
NlaIV GGNNCC 2 cut(s) 507, 1327
NmeAIII GCCGAG 2 cut(s) 486, 542
OliI CACNNNNGTG 1 cut(s) 53
PagI TCATGA 1 cut(s) 871
PcsI WCGNNNNNNNCGW 1 cut(s) 1256
PctI GAATGC 1 cut(s) 91
PdmI GAANNNNTTC 1 cut(s) 479
PfeI GAWTC 3 cut(s) 499, 736, 868
Pfl23II CGTACG 1 cut(s) 1178
PkrI GCNGC 8 cut(s) 7, 10, 200, 408, 459, 560, 744, 747
PleI GAGTC 6 cut(s) 223, 400, 739, 751, 1018, 1099
PpsI GAGTC 6 cut(s) 223, 400, 739, 751, 1018, 1099
PsiI TTATAA 1 cut(s) 1385
PspFI CCCAGC 3 cut(s) 19, 243, 809
PspLI CGTACG 1 cut(s) 1178
PspN4I GGNNCC 2 cut(s) 507, 1327
PspPI GGNCC 5 cut(s) 70, 121, 196, 279, 301
PstNI CAGNNNCTG 1 cut(s) 760
PsuI RGATCY 1 cut(s) 183
RsaI GTAC 3 cut(s) 212, 359, 1180
RsaNI GTAC 3 cut(s) 211, 358, 1179
RseI CAYNNNNRTG 2 cut(s) 53, 1344
SaqAI TTAA 1 cut(s) 1194
SatI GCNGC 8 cut(s) 6, 9, 199, 407, 458, 559, 743, 746
Sau3AI GATC 5 cut(s) 183, 318, 444, 468, 547
Sau96I GGNCC 5 cut(s) 70, 121, 196, 279, 301
SchI GAGTC 6 cut(s) 223, 401, 740, 751, 1019, 1100
ScrFI CCNGG 2 cut(s) 687, 688
SduI GDGCHC 1 cut(s) 687
SfaNI GCATC 1 cut(s) 1309
SfcI CTRYAG 1 cut(s) 1428
SmaI CCCGGG 1 cut(s) 688
SmiMI CAYNNNNRTG 2 cut(s) 53, 1344
SmlI CTYRAG 1 cut(s) 1490
SmoI CTYRAG 1 cut(s) 1490
Sse9I AATT 4 cut(s) 106, 306, 529, 830
SsiI CCGC 9 cut(s) 6, 9, 199, 558, 699, 740, 746, 773, 1419
SspMI CTAG 1 cut(s) 1026
StyD4I CCNGG 2 cut(s) 685, 686
StyI CCWWGG 2 cut(s) 192, 297
TaaI ACNGT 1 cut(s) 1239
TaqI TCGA 5 cut(s) 390, 395, 620, 734, 1061
TasI AATT 4 cut(s) 106, 306, 529, 830
TatI WGTACW 1 cut(s) 357
TauI GCSGC 5 cut(s) 8, 11, 201, 561, 748
TfiI GAWTC 3 cut(s) 499, 736, 868
Tru1I TTAA 1 cut(s) 1194
Tru9I TTAA 1 cut(s) 1194
TscAI CASTG 2 cut(s) 601, 901
TseI GCWGC 3 cut(s) 406, 457, 742
TspDTI ATGAA 3 cut(s) 860, 888, 1155
TspMI CCCGGG 1 cut(s) 686
TspRI CASTG 2 cut(s) 601, 901
XapI RAATTY 2 cut(s) 106, 306
XmaI CCCGGG 1 cut(s) 686
XmnI GAANNNNTTC 1 cut(s) 479
XspI CTAG 1 cut(s) 1026
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.