Rw2G044810

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005671
Physical Location & Seq
Forward (+)
71530832 .. 71536819
5988 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G044810.1

Sequence Viewer

Length: 2757 bp
ATGGAGAAGAGAGAAAAGGTGAAGTTGATAGTAGCGGCGGCCCTCGCTTCTCTCTCCGTCTTCTTCCTCTACCGGAGGAGACGCCGAAACTGCCCCCAAACTCACTGTTATTTACGAGACGACCCGAACAAGCCGCAGCACGGATTCAAGCGAGTCTTGGCCGACAATTCCTACTCTCCGTTCAAGCATTTCAACCCCGCCGACGACTCTAACGTCGGCGGAGCAGATAAGAATTGGAATTCGCATCCTTATGAGGCGGAGATCACGGCGGTGATGGAAAGTCCGCCGATTGAGGTGAAATGTGTGACGGAGAAGAATTTGGATTCGGAAATGAGGGAGGAGTATGTTTGGGTTGAGAATGAGACTCAGTTGAAGGAAATGGTTGAGGTTTTGAGTAAAGAGAAGATCTTTGGGGTTGATACTGAGCAGCATAGCTTAAGGTCATTTTTGGGATTTACTGCTTTGATACAGATTTCGACTGAGAAGGAAGATTATTTGGTGGACGCTATTGCTTTACATGACAGTATGAGCCTTTTGCAGCCGGTTTTCGCCAATGCTAGTATCTGCAAGGTGTTCCATGGGGCAGATAGTGATGTTCTCTGGCTGCAAAGGGACTTTCATATTTATGTCGTCAATATGTTTGATACTGCAAAGGCTTGTGAAGTGTTGTCAAAACCGCAGAAATCACTGGCTTATTTACTTGAATCTTACTGTGGAGTCGTCACTAACAAATTGTTTCAGCGTGAAGACTGGAGACAACGCCCTTTGTCAATGGAAATGGTGCAATATGCTCGGACAGATGCACACTATCTCCTTTACATTGCAGGCCGTTTACTAGCTGAGCTGGATGTGCTGGATAATGAAAACTCTTGTCCCAATGACAAATTCCGTTTTGTTCTCGAGGCTAGTCGGCGTTCAAACATGATATGTTTGCAACTGTATACAAAAGACGTAGAAGCTTCGCCTGGAGAGTCTGCTGCTTCATCAATATTTAGCCGTCATTTAAATGGCCAGGGAGGCATTTCATCAGCATCTTATGAAATCCAGAATTCTGTGAGACGACTTTGTGCATGGAGAGATTTGATGGCTCGTGTACATGATGAGAGCTTGAGATATGTAATATCAGATCAGGCTATTGTTGCCCTTGCAGAAAATGCTCCAACTAACCCTGCAGACATGTTAACCACCATTGCAGAAGCTGATGATAATGTGGATTCAAGTTTCAATTCTGGCCTCTCATCTCCCTCTCCTGTGGTCTGCAGTCATTTGAATGATTTTTATTATCTGCTCCAGAAGAAAGTAGGCAATCCTGATGATCTCTTACCCATGATTCTTCAGAACTGCCTGGGTCAAAAAGGGAGTTGCCCACTGTCTGTCTTCAACTATGCTTTACTGGTTAATTATAATATGAAAGTACCACTGGTTTCTAAACAAAATGGGGTCAGAAATCCAAAGCAAGTCGGTCGAATGGCTTCGCGAAAGCTGTTTGTTCAAAAATTTTCCTGCAAATCCCCAGTTTATCATAATTGCAGGATCTATGCAAATGATGGGCGTTTGCTGTGTTACTGTGACCGCAGAAAGCTTGAATGGTATCTTCATCGGAATTTGGCTAAACTTGTTGAGGACAATCCACCTGGAATTATGCTTCTTTTTGAGCCCAAAGGCCGTCCAGAAGATGAAGGCAATGATTTCTACATTCAAAGTAAGAAAAATATCTGTGTCGGCTGCGGTGAAAGAAATCATTACTTACGGTACCGAATTATTCCCTCATGCTACAGAATGCACTTCCCTGAACATTTGAAAAGCCACCGTTCTCATGATATTGTCCTACTATGCGTGGACTGTCACGAAATTGCTCATGCCGCTGCAGAGAAGTATAAAAGAAAGATTGCTGCAGAATTTGGGATTCCACTTTTTGTTCGTAAAGTGGTTGATTCACAACAAGCTCAATCTTTATCTGGGGCATCTGTGCCTGCAACAAATGTTGAGGAAGCAGGTGTATCTCCTTTACAGTTGCGAACTGCTGCTATGGCCCTTTTGCGCCATGGATCAACAATGCCATCCAAGCGCCGTGAGGAACTGACAGAGATTGTTATGACATTTTATGGAGGGAGGCAAATCTCTGAGGAAGATTTAGAAAAGGCTTTGCTTGTTGGGATGACTCCTCATGAGAAAAGACGACTTCAGAAGAAGAAAGGGTTTTCTTTTCAACATTCTAGAGGGAGTATTTCTTCAGATGCAGAGGAGAAAATTACTGTTAACATGTCCACATCTGCCACTCCAGATGCTTCAGAAGTTGATACTGTACATGAATCTTGCACCAATGAAGCCAAAGCATCTATGATAAACCAAGATGAAGATGTTTTCACAGTGAAGGATGCTGATTTAGGCAACTCTGCTTTAGATTTGAGGATTGGGGAAAAAGCATCTCCAAGCAATGAGAAGAACATGAGTTCAGAGATAACCAGAGTGTCCGATACTACACTTGTTTCTGTTGTAAATGGTGATGGTATTTATGACCGTAGAAGTACACCAAATGGATCAGTAGACTTCCGTTCTACTTCCAAACGTAATCATAAATCAAAACACTCCCTCTTAGGACATGGTCCACATGGAAGGCAAGTTGTGGATCATCTGTTAAAGGAATCTGGAGAAGATGGTATTAATCAGTTCTGTCAAAGGTGGAGACAAGTATTTGTTGAAACTGTTCATCCCCGTTTTTTACCTGCCGGCTGGGATATTACACACAGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0000178 GO:0000460 GO:0000785 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006403 GO:0006725 GO:0006807 GO:0007549 GO:0008150 GO:0008152 GO:0008298 GO:0008334 GO:0008408 GO:0009048 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010639 GO:0016070 GO:0016071 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032210 GO:0032211 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035327 GO:0040029 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045934 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051179 GO:0051641 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071025 GO:0071027 GO:0071028 GO:0071029 GO:0071030 GO:0071033 GO:0071034 GO:0071035 GO:0071043 GO:0071044 GO:0071046 GO:0071048 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902494 GO:1904356 GO:1904357 GO:1904872 GO:1905354 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1 HRDC

Protein Analysis

918

Amino Acids

103.83

Weight (kDa)

6.9

Isoelectric Point (pI)

50.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 115 - 282 4.4e-50 3'-5' exonuclease
HRDC PF00570 352 - 395 4.4e-09 HRDC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1404
AarI CACCTGC 1 cut(s) 1985
AasI GACNNNNNNGTC 1 cut(s) 212
Acc36I ACCTGC 2 cut(s) 1985, 2734
Acc65I GGTACC 1 cut(s) 1752
AccB1I GGYRCC 1 cut(s) 1752
AccI GTMKAC 2 cut(s) 941, 2547
AccII CGCG 1 cut(s) 1477
AclWI GGATC 4 cut(s) 1541, 2056, 2548, 2637
AcoI YGGCCR 2 cut(s) 159, 1009
AcsI RAATTY 7 cut(s) 238, 316, 884, 1048, 1496, 1603, 1898
AcuI CTGAAG 4 cut(s) 1319, 2168, 2217, 2274
AcyI GRCGYC 1 cut(s) 82
AfaI GTAC 5 cut(s) 1095, 1416, 1754, 2307, 2530
AfiI CCNNNNNNNGG 1 cut(s) 140
AflII CTTAAG 1 cut(s) 436
AflIII ACRYGT 2 cut(s) 1176, 2262
AjnI CCWGG 4 cut(s) 964, 1011, 1344, 1633
AjuI GAANNNNNNNTTGG 2 cut(s) 869, 901
AleI CACNNNNGTG 1 cut(s) 269
AluBI AGCT 9 cut(s) 435, 839, 844, 959, 1107, 1199, 1483, 1582, 1946
AluI AGCT 9 cut(s) 435, 839, 844, 959, 1107, 1199, 1483, 1582, 1946
Alw26I GTCTC 6 cut(s) 73, 111, 356, 748, 1051, 2680
AlwI GGATC 4 cut(s) 1541, 2056, 2548, 2637
AlwNI CAGNNNCTG 1 cut(s) 1199
Ama87I CYCGRG 1 cut(s) 899
AoxI GGCC 7 cut(s) 39, 159, 826, 1009, 1231, 1663, 2031
ApeKI GCWGC 9 cut(s) 136, 427, 538, 604, 977, 1725, 1865, 1892, 2024
ApoI RAATTY 7 cut(s) 238, 316, 884, 1048, 1496, 1603, 1898
AseI ATTAAT 1 cut(s) 2664
Asp700I GAANNNNTTC 2 cut(s) 1471, 2706
Asp718I GGTACC 1 cut(s) 1752
AspLEI GCGC 2 cut(s) 2043, 2070
AspS9I GGNCC 3 cut(s) 40, 2032, 2606
AsuHPI GGTGA 5 cut(s) 31, 283, 307, 1742, 2516
AvaI CYCGRG 1 cut(s) 899
AvaII GGWCC 1 cut(s) 2606
BalI TGGCCA 1 cut(s) 1011
BanI GGYRCC 1 cut(s) 1752
BanII GRGCYC 1 cut(s) 1659
BauI CACGAG 1 cut(s) 1089
BbsI GAAGAC 3 cut(s) 52, 753, 1369
BbvI GCAGC 9 cut(s) 148, 439, 550, 591, 964, 1712, 1852, 1879, 2011
BccI CCATC 6 cut(s) 268, 1078, 1541, 2068, 2501, 2651
BceAI ACGGC 5 cut(s) 282, 813, 981, 1650, 2055
BcgI CGANNNNNNTGC 4 cut(s) 773, 807, 1445, 1479
BciT130I CCWGG 4 cut(s) 966, 1013, 1346, 1635
BcoDI GTCTC 6 cut(s) 73, 111, 356, 748, 1051, 2680
BfaI CTAG 4 cut(s) 558, 836, 906, 2217
BfmI CTRYAG 5 cut(s) 1170, 1258, 1774, 1866, 1893
BfoI RGCGCY 1 cut(s) 2071
BfrI CTTAAG 1 cut(s) 436
BfuAI ACCTGC 2 cut(s) 1985, 2734
BglI GCCNNNNNGGC 1 cut(s) 1017
BglII AGATCT 1 cut(s) 405
BlpI GCTNAGC 1 cut(s) 840
Bme1390I CCNGG 4 cut(s) 966, 1013, 1346, 1635
Bme18I GGWCC 1 cut(s) 2606
BmeT110I CYCGRG 1 cut(s) 899
BmgT120I GGNCC 3 cut(s) 40, 2032, 2606
BmiI GGNNCC 1 cut(s) 1754
BmrFI CCNGG 4 cut(s) 966, 1013, 1346, 1635
BmrI ACTGGG 1 cut(s) 1508
BmsI GCATC 9 cut(s) 253, 790, 1040, 1973, 2227, 2275, 2345, 2368, 2435
BmuI ACTGGG 1 cut(s) 1508
BpiI GAAGAC 3 cut(s) 52, 753, 1369
BpmI CTGGAG 5 cut(s) 772, 987, 1274, 2265, 2670
Bpu1102I GCTNAGC 1 cut(s) 840
BpuEI CTTGAG 1 cut(s) 1129
BsaHI GRCGYC 1 cut(s) 82
BsaJI CCNNGG 4 cut(s) 577, 1012, 1345, 2044
BsaWI WCCGGW 1 cut(s) 72
BsaXI ACNNNNNCTCC 2 cut(s) 795, 825
Bsc4I CCNNNNNNNGG 1 cut(s) 140
Bse118I RCCGGY 2 cut(s) 541, 2729
Bse1I ACTGG 5 cut(s) 693, 755, 1398, 1425, 1514
Bse3DI GCAATG 4 cut(s) 819, 1188, 1690, 2443
BseBI CCWGG 4 cut(s) 966, 1013, 1346, 1635
BseDI CCNNGG 4 cut(s) 577, 1012, 1345, 2044
BseGI GGATG 6 cut(s) 244, 853, 2060, 2163, 2383, 2710
BseLI CCNNNNNNNGG 1 cut(s) 140
BseMI GCAATG 4 cut(s) 819, 1188, 1690, 2443
BseMII CTCAG 5 cut(s) 380, 414, 471, 831, 2115
BseNI ACTGG 5 cut(s) 693, 755, 1398, 1425, 1514
BseRI GAGGAG 4 cut(s) 91, 353, 2154, 2258
BseXI GCAGC 9 cut(s) 148, 439, 550, 591, 964, 1712, 1852, 1879, 2011
BseYI CCCAGC 1 cut(s) 2733
Bsh1236I CGCG 1 cut(s) 1477
Bsh1285I CGRYCG 1 cut(s) 1465
BshFI GGCC 7 cut(s) 41, 161, 828, 1011, 1233, 1665, 2033
BshNI GGYRCC 1 cut(s) 1752
BsiEI CGRYCG 1 cut(s) 1465
BsiHKCI CYCGRG 1 cut(s) 899
BsiSI CCGG 3 cut(s) 73, 542, 2730
BslFI GGGAC 2 cut(s) 626, 858
BslI CCNNNNNNNGG 1 cut(s) 140
BsmAI GTCTC 6 cut(s) 73, 111, 356, 748, 1051, 2680
BsmBI CGTCTC 3 cut(s) 73, 111, 1051
BsmFI GGGAC 2 cut(s) 626, 858
BsmI GAATGC 1 cut(s) 1785
BsnI GGCC 7 cut(s) 41, 161, 828, 1011, 1233, 1665, 2033
BsoBI CYCGRG 1 cut(s) 899
Bsp1286I GDGCHC 1 cut(s) 1659
Bsp1407I TGTACA 2 cut(s) 1093, 2305
Bsp143I GATC 8 cut(s) 261, 405, 1126, 1315, 1533, 2048, 2540, 2629
Bsp1720I GCTNAGC 1 cut(s) 840
Bsp19I CCATGG 2 cut(s) 577, 2044
Bsp68I TCGCGA 1 cut(s) 1477
BspANI GGCC 7 cut(s) 41, 161, 828, 1011, 1233, 1665, 2033
BspCNI CTCAG 5 cut(s) 379, 415, 472, 832, 2116
BspFNI CGCG 1 cut(s) 1477
BspHI TCATGA 2 cut(s) 1816, 2167
BspLI GGNNCC 1 cut(s) 1754
BspMAI CTGCAG 4 cut(s) 1174, 1262, 1870, 1897
BspMI ACCTGC 2 cut(s) 1985, 2734
BspPI GGATC 4 cut(s) 1541, 2056, 2548, 2637
BspT107I GGYRCC 1 cut(s) 1752
BspTI CTTAAG 1 cut(s) 436
BsrDI GCAATG 4 cut(s) 819, 1188, 1690, 2443
BsrFI RCCGGY 2 cut(s) 541, 2729
BsrGI TGTACA 2 cut(s) 1093, 2305
BsrI ACTGG 5 cut(s) 693, 755, 1398, 1425, 1514
BssAI RCCGGY 2 cut(s) 541, 2729
BssECI CCNNGG 4 cut(s) 577, 1012, 1345, 2044
BssMI GATC 8 cut(s) 261, 405, 1126, 1315, 1533, 2048, 2540, 2629
BssNAI GTATAC 1 cut(s) 942
BssNI GRCGYC 1 cut(s) 82
BssSI CACGAG 1 cut(s) 1089
BssT1I CCWWGG 2 cut(s) 577, 2044
Bst1107I GTATAC 1 cut(s) 942
Bst2BI CACGAG 1 cut(s) 1089
Bst2UI CCWGG 4 cut(s) 966, 1013, 1346, 1635
Bst6I CTCTTC 1 cut(s) 2
BstACI GRCGYC 1 cut(s) 82
BstAFI CTTAAG 1 cut(s) 436
BstAPI GCANNNNNTGC 1 cut(s) 1154
BstAUI TGTACA 2 cut(s) 1093, 2305
BstC8I GCNNGC 3 cut(s) 826, 1974, 2731
BstDEI CTNAG 6 cut(s) 366, 423, 480, 840, 2124, 2596
BstDSI CCRYGG 2 cut(s) 577, 2044
BstF5I GGATG 6 cut(s) 244, 853, 2060, 2163, 2383, 2710
BstFNI CGCG 1 cut(s) 1477
BstH2I RGCGCY 1 cut(s) 2071
BstHHI GCGC 2 cut(s) 2043, 2070
BstKTI GATC 8 cut(s) 264, 408, 1129, 1318, 1536, 2051, 2543, 2632
BstMAI GTCTC 6 cut(s) 73, 111, 356, 748, 1051, 2680
BstMBI GATC 8 cut(s) 261, 405, 1126, 1315, 1533, 2048, 2540, 2629
BstMCI CGRYCG 1 cut(s) 1465
BstMWI GCNNNNNNNGC 9 cut(s) 44, 90, 850, 1017, 1139, 1154, 1862, 2030, 2065
BstNI CCWGG 4 cut(s) 966, 1013, 1346, 1635
BstNSI RCATGY 2 cut(s) 1180, 2266
BstSCI CCNGG 4 cut(s) 964, 1011, 1344, 1633
BstSFI CTRYAG 5 cut(s) 1170, 1258, 1774, 1866, 1893
BstUI CGCG 1 cut(s) 1477
BstV1I GCAGC 9 cut(s) 148, 439, 550, 591, 964, 1712, 1852, 1879, 2011
BstV2I GAAGAC 3 cut(s) 52, 753, 1369
BstX2I RGATCY 2 cut(s) 405, 1533
BstYI RGATCY 2 cut(s) 405, 1533
BstZ17I GTATAC 1 cut(s) 942
BsuRI GGCC 7 cut(s) 41, 161, 828, 1011, 1233, 1665, 2033
BtgI CCRYGG 2 cut(s) 577, 2044
BtsCI GGATG 6 cut(s) 244, 853, 2060, 2163, 2383, 2710
BtsIMutI CAGTG 5 cut(s) 103, 686, 1367, 1418, 2376
BtuMI TCGCGA 1 cut(s) 1477
BveI ACCTGC 2 cut(s) 1985, 2734
Cac8I GCNNGC 3 cut(s) 826, 1974, 2731
CaiI CAGNNNCTG 1 cut(s) 1199
CciI TCATGA 2 cut(s) 1816, 2167
CfoI GCGC 2 cut(s) 2043, 2070
Cfr10I RCCGGY 2 cut(s) 541, 2729
Cfr13I GGNCC 3 cut(s) 40, 2032, 2606
CseI GACGC 2 cut(s) 90, 512
Csp6I GTAC 5 cut(s) 1094, 1415, 1753, 2306, 2529
CviQI GTAC 5 cut(s) 1094, 1415, 1753, 2306, 2529
DdeI CTNAG 6 cut(s) 366, 423, 480, 840, 2124, 2596
DpnI GATC 8 cut(s) 263, 407, 1128, 1317, 1535, 2050, 2542, 2631
DpnII GATC 8 cut(s) 261, 405, 1126, 1315, 1533, 2048, 2540, 2629
DraI TTTAAA 1 cut(s) 1005
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
EaeI YGGCCR 2 cut(s) 159, 1009
Eam1104I CTCTTC 1 cut(s) 2
EarI CTCTTC 1 cut(s) 2
EciI GGCGGA 3 cut(s) 234, 272, 273
Eco130I CCWWGG 2 cut(s) 577, 2044
Eco24I GRGCYC 1 cut(s) 1659
Eco47I GGWCC 1 cut(s) 2606
Eco57I CTGAAG 4 cut(s) 1319, 2168, 2217, 2274
Eco88I CYCGRG 1 cut(s) 899
EcoRI GAATTC 2 cut(s) 238, 1048
EcoRII CCWGG 4 cut(s) 964, 1011, 1344, 1633
EcoT14I CCWWGG 2 cut(s) 577, 2044
EcoT38I GRGCYC 1 cut(s) 1659
ErhI CCWWGG 2 cut(s) 577, 2044
Esp3I CGTCTC 3 cut(s) 73, 111, 1051
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FaqI GGGAC 2 cut(s) 626, 858
FauI CCCGC 1 cut(s) 205
FblI GTMKAC 2 cut(s) 941, 2547
FokI GGATG 6 cut(s) 231, 860, 2047, 2170, 2390, 2697
FriOI GRGCYC 1 cut(s) 1659
FspBI CTAG 4 cut(s) 558, 836, 906, 2217
GlaI GCGC 2 cut(s) 2042, 2069
GsaI CCCAGC 1 cut(s) 2737
GsuI CTGGAG 5 cut(s) 772, 987, 1274, 2265, 2670
HaeII RGCGCY 1 cut(s) 2071
HaeIII GGCC 7 cut(s) 41, 161, 828, 1011, 1233, 1665, 2033
HapII CCGG 3 cut(s) 73, 542, 2730
HgaI GACGC 2 cut(s) 90, 512
HhaI GCGC 2 cut(s) 2043, 2070
Hin1I GRCGYC 1 cut(s) 82
Hin6I GCGC 2 cut(s) 2041, 2068
HinP1I GCGC 2 cut(s) 2041, 2068
HincII GTYRAC 2 cut(s) 1182, 2260
HindII GTYRAC 2 cut(s) 1182, 2260
HindIII AAGCTT 2 cut(s) 957, 1580
HpaI GTTAAC 2 cut(s) 1182, 2260
HpaII CCGG 3 cut(s) 73, 542, 2730
HphI GGTGA 5 cut(s) 31, 283, 307, 1742, 2516
Hpy99I CGWCG 2 cut(s) 206, 218
HpyAV CCTTC 5 cut(s) 367, 478, 1673, 2368, 2610
HpyCH4IV ACGT 3 cut(s) 213, 951, 2569
HpyF10VI GCNNNNNNNGC 9 cut(s) 44, 90, 850, 1017, 1139, 1154, 1862, 2030, 2065
HpyF3I CTNAG 6 cut(s) 366, 423, 480, 840, 2124, 2596
HpySE526I ACGT 3 cut(s) 213, 951, 2569
Hsp92I GRCGYC 1 cut(s) 82
HspAI GCGC 2 cut(s) 2041, 2068
KpnI GGTACC 1 cut(s) 1756
KroI GCCGGC 1 cut(s) 2729
KroNI GCCGGC 1 cut(s) 2731
KspAI GTTAAC 2 cut(s) 1182, 2260
Kzo9I GATC 8 cut(s) 261, 405, 1126, 1315, 1533, 2048, 2540, 2629
LmnI GCTCC 3 cut(s) 221, 1162, 1293
Lsp1109I GCAGC 9 cut(s) 148, 439, 550, 591, 964, 1712, 1852, 1879, 2011
LweI GCATC 9 cut(s) 253, 790, 1040, 1973, 2227, 2275, 2345, 2368, 2435
MaeI CTAG 4 cut(s) 558, 836, 906, 2217
MaeII ACGT 3 cut(s) 213, 951, 2569
MaeIII GTNAC 5 cut(s) 304, 721, 1562, 1568, 1844
MalI GATC 8 cut(s) 263, 407, 1128, 1317, 1535, 2050, 2542, 2631
MboI GATC 8 cut(s) 261, 405, 1126, 1315, 1533, 2048, 2540, 2629
MflI RGATCY 2 cut(s) 405, 1533
MhlI GDGCHC 1 cut(s) 1659
MlsI TGGCCA 1 cut(s) 1011
MluNI TGGCCA 1 cut(s) 1011
MlyI GAGTC 6 cut(s) 162, 200, 358, 726, 980, 2155
MmeI TCCRAC 1 cut(s) 1184
Mox20I TGGCCA 1 cut(s) 1011
MroNI GCCGGC 1 cut(s) 2729
MroXI GAANNNNTTC 2 cut(s) 1471, 2706
MscI TGGCCA 1 cut(s) 1011
MseI TTAA 7 cut(s) 437, 1004, 1181, 1398, 2259, 2639, 2664
MslI CAYNNNNRTG 5 cut(s) 249, 269, 624, 1005, 1269
Msp20I TGGCCA 1 cut(s) 1011
MspA1I CMGCKG 1 cut(s) 1865
MspCI CTTAAG 1 cut(s) 436
MspI CCGG 3 cut(s) 73, 542, 2730
MspR9I CCNGG 4 cut(s) 966, 1013, 1346, 1635
Mva1269I GAATGC 1 cut(s) 1785
MvaI CCWGG 4 cut(s) 966, 1013, 1346, 1635
MvnI CGCG 1 cut(s) 1477
MwoI GCNNNNNNNGC 9 cut(s) 44, 90, 850, 1017, 1139, 1154, 1862, 2030, 2065
NaeI GCCGGC 1 cut(s) 2731
NcoI CCATGG 2 cut(s) 577, 2044
NdeII GATC 8 cut(s) 261, 405, 1126, 1315, 1533, 2048, 2540, 2629
NgoMIV GCCGGC 1 cut(s) 2729
NlaIV GGNNCC 1 cut(s) 1754
NmuCI GTSAC 4 cut(s) 304, 721, 1568, 1844
NruI TCGCGA 1 cut(s) 1477
NspI RCATGY 2 cut(s) 1180, 2266
OliI CACNNNNGTG 1 cut(s) 269
PaeR7I CTCGAG 1 cut(s) 899
PagI TCATGA 2 cut(s) 1816, 2167
PaqCI CACCTGC 1 cut(s) 1985
PciI ACATGT 2 cut(s) 1176, 2262
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PctI GAATGC 1 cut(s) 1785
PdiI GCCGGC 1 cut(s) 2731
PdmI GAANNNNTTC 2 cut(s) 1471, 2706
PfeI GAWTC 9 cut(s) 144, 323, 704, 1214, 1330, 1906, 1934, 2312, 2645
PflFI GACNNNGTC 1 cut(s) 2604
PleI GAGTC 6 cut(s) 161, 200, 358, 725, 979, 2155
PpsI GAGTC 6 cut(s) 161, 200, 358, 725, 979, 2155
PscI ACATGT 2 cut(s) 1176, 2262
PshBI ATTAAT 1 cut(s) 2664
PsiI TTATAA 1 cut(s) 1404
Psp6I CCWGG 4 cut(s) 964, 1011, 1344, 1633
PspFI CCCAGC 1 cut(s) 2733
PspGI CCWGG 4 cut(s) 964, 1011, 1344, 1633
PspN4I GGNNCC 1 cut(s) 1754
PspPI GGNCC 3 cut(s) 40, 2032, 2606
PstI CTGCAG 4 cut(s) 1174, 1262, 1870, 1897
PstNI CAGNNNCTG 1 cut(s) 1199
PsuI RGATCY 2 cut(s) 405, 1533
PsyI GACNNNGTC 1 cut(s) 2604
RruI TCGCGA 1 cut(s) 1477
RsaI GTAC 5 cut(s) 1095, 1416, 1754, 2307, 2530
RsaNI GTAC 5 cut(s) 1094, 1415, 1753, 2306, 2529
RseI CAYNNNNRTG 5 cut(s) 249, 269, 624, 1005, 1269
SaqAI TTAA 7 cut(s) 437, 1004, 1181, 1398, 2259, 2639, 2664
Sau3AI GATC 8 cut(s) 261, 405, 1126, 1315, 1533, 2048, 2540, 2629
Sau96I GGNCC 3 cut(s) 40, 2032, 2606
SchI GAGTC 6 cut(s) 162, 200, 358, 726, 980, 2155
ScrFI CCNGG 4 cut(s) 966, 1013, 1346, 1635
SduI GDGCHC 1 cut(s) 1659
SfaNI GCATC 9 cut(s) 253, 790, 1040, 1973, 2227, 2275, 2345, 2368, 2435
SfcI CTRYAG 5 cut(s) 1170, 1258, 1774, 1866, 1893
Sfr274I CTCGAG 1 cut(s) 899
SinI GGWCC 1 cut(s) 2606
SlaI CTCGAG 1 cut(s) 899
SmiI ATTTAAAT 1 cut(s) 1005
SmiMI CAYNNNNRTG 5 cut(s) 249, 269, 624, 1005, 1269
SmlI CTYRAG 3 cut(s) 436, 899, 1108
SmoI CTYRAG 3 cut(s) 436, 899, 1108
SspI AATATT 1 cut(s) 990
SspMI CTAG 4 cut(s) 558, 836, 906, 2217
StyD4I CCNGG 4 cut(s) 964, 1011, 1344, 1633
StyI CCWWGG 2 cut(s) 577, 2044
SwaI ATTTAAAT 1 cut(s) 1005
TaiI ACGT 3 cut(s) 216, 954, 2572
TaqI TCGA 3 cut(s) 476, 900, 1465
TaqII GACCGA 1 cut(s) 1451
TatI WGTACW 3 cut(s) 1093, 2305, 2528
TauI GCSGC 4 cut(s) 38, 41, 136, 1865
TfiI GAWTC 9 cut(s) 144, 323, 704, 1214, 1330, 1906, 1934, 2312, 2645
Tru1I TTAA 7 cut(s) 437, 1004, 1181, 1398, 2259, 2639, 2664
Tru9I TTAA 7 cut(s) 437, 1004, 1181, 1398, 2259, 2639, 2664
TscAI CASTG 5 cut(s) 110, 693, 1374, 1425, 2376
TseFI GTSAC 4 cut(s) 304, 721, 1568, 1844
TseI GCWGC 9 cut(s) 136, 427, 538, 604, 977, 1725, 1865, 1892, 2024
Tsp45I GTSAC 4 cut(s) 304, 721, 1568, 1844
TspGWI ACGGA 6 cut(s) 46, 156, 168, 323, 878, 2543
TspRI CASTG 5 cut(s) 110, 693, 1374, 1425, 2376
Tth111I GACNNNGTC 1 cut(s) 2604
Vha464I CTTAAG 1 cut(s) 436
VpaK11BI GGWCC 1 cut(s) 2606
VspI ATTAAT 1 cut(s) 2664
XapI RAATTY 7 cut(s) 238, 316, 884, 1048, 1496, 1603, 1898
XbaI TCTAGA 1 cut(s) 2216
XceI RCATGY 2 cut(s) 1180, 2266
XhoI CTCGAG 1 cut(s) 899
XmiI GTMKAC 2 cut(s) 941, 2547
XmnI GAANNNNTTC 2 cut(s) 1471, 2706
XspI CTAG 4 cut(s) 558, 836, 906, 2217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.