Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0007593

Physical Location & Seq

Genomic Coordinates Reverse (-)

10176133 .. 10183600

Gene Structure 7468 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1132600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2328 bp

ATGAGCCAAGCAATGAGGCCACCAACCGCAGCCCTTTACCTTCTCTCCTTCCTTCTCTCCTCTCTAGTACTCCATACCCCCACCTTTGCCATAAAAAAGTCATATGTGGTATACCTGGGATCACACTCACATCCCCCAAACTTGTCAGAACTTGAACTAAACCAAGTCACAGACAATCACCATGAGTTTCTTGGTTCCTTCTTGGGCAGCCATGAAGTTGCAAAAGAATCCATGTTTTACTCATACACAAGGCACATCAATGGCTTTGCTGCTACACTAGAAGAAGAAGAGGCAGCTCAGATTGCTAAGCATCCAAAGGTAGTCTCAGTATTCTTGAACCAGGGAAGAAAATTACACACAACTCGATCGTGGGATTTCTTGGGACTTGAGCAAGATGGTGTTGTTTCACCCAATTCAATCTGGAAGAAGGCAAGATACGGTGAAGATTCAATCATAGGAAACCTTGATACTGGCGCATGGCCAGAATCCAAGAGCTTTAGTGATGAAGGGTATGGACCGATTCCATCCAAGTGGAAAGGAATCTGCCAAAATGAGACAGATTCTGAATTTCACTGCAATAGGAAGCTTATTGGAGCACGATACTTCAACAAGGGCTATGCTGCAGCTGCTGGTCCCCTCAACTCCTCCTTCGATTCGCCGCGTGACAACGATGGCCATGGCTCCCACACCTTGTCAACCGCTGGTGGCAATTTTGTAACCGGTGCGAGCGTGTTCGGCTTTGGAAATGGAACAGCAAAGGGTGGATCACCAAAAGCCAGGGTTGCAGCCTATAAGGTGTGCTGGTCTCCAGTGAATGGAAGTGAGTGCTTTCATGCAGATATATTGGCTGCATTTGATGTGGCCATCCATGACGGTGTTGATGTGTTGTCCGTTTCACTGGGTGGAGATCCTACTACATTCTTCAGTGACAGTGTTTCAATTGGTGCTTTTCATGCTGTTAAGCATGGCATTGTTGTGGTTTGCTCAGCGGGAAATTCTGGTCCGGCTGAAGGTACTGTCTTGAATATATCACCGTGGCAGATCACAGTCGGCGCCAGTACTATGGATAGGGAGTTTCCGAGTTATGTCACCCTCGGCAACTGGAAGCACCTCAAGGGACAAAGCTTATCGTCTAGTGCCTTGCAGAGCAAGAAATTTTACCAACTTATTAGTGCTGCGGATGCCAAAGCAGCTAATGCATCAGCTAAAGAAGCTCTGCTATGCAAGGCCGGCACGCTTGATCCTAAAAAAGTGAAGGGAAAGATCTTGGCCTGCCTGCGAGGGGACAACGCAAGAGTGGACAAGGGTGAGCAAGCCTTACTGGCTGGCGCCGTGGGAATGATTCTTGCTAACAATGCTATTAACGGGAACGAAATTATTTCTGATCCACATGTCCTCCCTGCTTCACATATCAATTTCACCGACGGCATCCTTGTCTTTGCTTATATCAATTCAACTAAGTCTCCGCGGGCTTACATAAAGCGTGCAATAACGGAACTGGGAACAAAGCCATCTCCATTCATGGCAGCATTTTCATCAAAGGGACCCAACACCATTACACCAGGCATCCTTAAGCCTGATATCACTGCACCTGGAGTGAGTGTTATAGCAGCCTACACGAAAGCACAAGGGCCAACAAATCAAATGTTTGACAAACGGCGAATTCCATTCAACTCAGTGTCAGGCACTTCAATGTCATGTCCACATATTTCCGGCATTTGTGGCCTTCTTAAAACCCTCTATCCTCATTGGAGTCCTGCAGCCATTAAATCTGCAATCATGACCACCGCGATAACACGAGATAACAGCATGGAACCATTGCTCAATGCTTCTTTCTATGAGGCAACACCATTTAGTTACGGCGCAGGACATGTTAACCCAAACAGTGCTATGGATCCTGGATTGGTATACGATTTATCCCATAACGATTACTTGAACTTCCTGTGCGCAATGGGCTACAACGAAACAGAAATGGAAATGTTTTCAGAGGATACGTATGAGTGCCCCAAACCAGCTATTAGTCCCACTAACCTCAACTACCCCTCAATCACTGTCCCTAAACTCTCTGGATCCCTTGAGGTGACTAGAACAGTGAAAAATGTTGGCTCTCCAGGAACATATAAGGCGAATATCCAAAGCCCGGATGGGGTATCGATTTCTGTCGAGCCAAAGAAGTTGAAGTTCATGAAAATTGGTGAAGAGAAGAGCTTTAAGGTACTCCTGCAGGTTAAAGAAGCAAACCAAGCTAAAAACTATGTGTTTGGGAAGTTGATATGGTCAGATGGTAAACACTATGTGAGGAGTCCAATTGTAGTCAAAGCTTCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0016787 GO:0017171 GO:0019538 GO:0022622 GO:0032501 GO:0032502 GO:0042221 GO:0043170 GO:0044238 GO:0048364 GO:0048527 GO:0048528 GO:0048731 GO:0048856 GO:0050896 GO:0070011 GO:0071704 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140096 GO:1901564 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

776

Amino Acids

83.32

Weight (kDa)

8.5

Isoelectric Point (pI)

34.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	34 - 119	4.9e-18	Peptidase inhibitor I9
MORF_dom	PF21864	49 - 111	2.6e-06	Multiple organellar RNA editing factor 1, MORF domain
Peptidase_S8	PF00082	151 - 623	1.9e-36	Subtilase family
PA	PF02225	405 - 476	2e-11	PA domain
fn3_6	PF17766	677 - 772	1.5e-32	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007593)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04160
fragaria_vesca	FvH4_6g40300
malus_domestica	MD09G1132200.v1.1 MD09G1132600.v1.1 MD17G1120300.v1.1
prunus_persica	Prupe.3G147300_v2.0.a1 Prupe.3G193800_v2.0.a1
pyrus_communis	pycom09g05410 pycom17g11090
rosa_chinensis	RchiOBHm_Chr2g0155491
rosa_laevigata	RLG00000020840
rosa_multiflora	Rmu_sc0003439.1_g000012
rosa_roxburghii	Rroxscaffold_2G00093670
rosa_rugosa	Rorug02G0447200 Rorug02G0447200
rosa_samantha	Rh2AG513600 Rh2AG513700 Rh2BG524400 Rh2BG524500 Rh2BG524600 Rh2CG498500 Rh2CG498600 Rh2DG533600
rosa_wichuraiana	Rw2G042220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1948
Acc36I	ACCTGC	1 cut(s)	2215
AccB1I	GGYRCC	2 cut(s)	1052, 1328
AccB7I	CCANNNNNTGG	1 cut(s)	815
AccI	GTMKAC	2 cut(s)	111, 1908
AccII	CGCG	3 cut(s)	661, 1468, 1790
AciI	CCGC	8 cut(s)	27, 659, 699, 989, 1178, 1466, 1468, 1788
AclWI	GGATC	9 cut(s)	127, 772, 902, 1235, 1379, 1889, 1902, 2064, 2077
AcoI	YGGCCR	3 cut(s)	479, 673, 861
AcsI	RAATTY	4 cut(s)	566, 994, 1154, 1662
AcuI	CTGAAG	2 cut(s)	907, 1029
AcyI	GRCGYC	2 cut(s)	1053, 1329
AdeI	CACNNNGTG	2 cut(s)	902, 2296
AfaI	GTAC	4 cut(s)	69, 1015, 1060, 2217
AfiI	CCNNNNNNNGG	5 cut(s)	815, 1010, 1282, 2140, 2146
AflII	CTTAAG	1 cut(s)	1571
AflIII	ACRYGT	2 cut(s)	1390, 1870
AgeI	ACCGGT	1 cut(s)	719
AjnI	CCWGG	7 cut(s)	114, 339, 776, 1561, 1591, 1897, 2110
Alw21I	GWGCWC	1 cut(s)	598
Alw26I	GTCTC	4 cut(s)	328, 548, 810, 1467
AlwI	GGATC	9 cut(s)	127, 772, 902, 1235, 1379, 1889, 1902, 2064, 2077
AlwNI	CAGNNNCTG	2 cut(s)	563, 629
AoxI	GGCC	8 cut(s)	17, 479, 673, 861, 1227, 1269, 1631, 1723
ApoI	RAATTY	4 cut(s)	566, 994, 1154, 1662
AsiGI	ACCGGT	1 cut(s)	719
AspLEI	GCGC	5 cut(s)	476, 1055, 1331, 1865, 1949
AspS9I	GGNCC	5 cut(s)	515, 632, 1001, 1544, 1631
AsuC2I	CCSGG	1 cut(s)	2141
AvaII	GGWCC	4 cut(s)	515, 632, 1001, 1544
BaeGI	GKGCMC	1 cut(s)	2006
BalI	TGGCCA	3 cut(s)	481, 675, 863
BamHI	GGATCC	2 cut(s)	1894, 2069
BanI	GGYRCC	2 cut(s)	1052, 1328
BarI	GAAGNNNNNNTAC	2 cut(s)	419, 451
BauI	CACGAG	1 cut(s)	1797
Bbv12I	GWGCWC	1 cut(s)	598
BccI	CCATC	7 cut(s)	389, 532, 665, 872, 1519, 2138, 2276
BceAI	ACGGC	4 cut(s)	1316, 1441, 1673, 1876
BciT130I	CCWGG	7 cut(s)	116, 341, 778, 1563, 1593, 1899, 2112
BciVI	GTATCC	1 cut(s)	1984
BcnI	CCSGG	1 cut(s)	2141
BcoDI	GTCTC	4 cut(s)	328, 548, 810, 1467
BfaI	CTAG	4 cut(s)	65, 278, 1134, 2085
BfmI	CTRYAG	3 cut(s)	621, 1758, 2222
BfoI	RGCGCY	2 cut(s)	1056, 1332
BfrI	CTTAAG	1 cut(s)	1571
BfuAI	ACCTGC	1 cut(s)	2215
BfuI	GTATCC	1 cut(s)	1984
BglI	GCCNNNNNGGC	1 cut(s)	1322
BglII	AGATCT	1 cut(s)	1263
BlpI	GCTNAGC	2 cut(s)	306, 985
BmcAI	AGTACT	2 cut(s)	69, 1060
Bme1390I	CCNGG	8 cut(s)	116, 341, 778, 1563, 1593, 1899, 2112, 2141
Bme18I	GGWCC	4 cut(s)	515, 632, 1001, 1544
BmgT120I	GGNCC	5 cut(s)	515, 632, 1001, 1544, 1631
BmrFI	CCNGG	8 cut(s)	116, 341, 778, 1563, 1593, 1899, 2112, 2141
BmrI	ACTGGG	2 cut(s)	908, 1508
BmsI	GCATC	5 cut(s)	319, 1171, 1208, 1437, 1575
BmuI	ACTGGG	2 cut(s)	908, 1508
BpmI	CTGGAG	3 cut(s)	792, 1614, 2094
Bpu1102I	GCTNAGC	2 cut(s)	306, 985
BpuEI	CTTGAG	3 cut(s)	407, 1097, 2096
BpuMI	CCSGG	1 cut(s)	2141
Bsa29I	ATCGAT	1 cut(s)	2153
BsaAI	YACGTR	1 cut(s)	1995
BsaHI	GRCGYC	2 cut(s)	1053, 1329
BsaI	GGTCTC	1 cut(s)	810
BsaJI	CCNNGG	8 cut(s)	115, 340, 676, 777, 1034, 1093, 1332, 1466
BsaWI	WCCGGW	1 cut(s)	719
BsaXI	ACNNNNNCTCC	8 cut(s)	29, 59, 1380, 1410, 1447, 1477, 1587, 1617
Bsc4I	CCNNNNNNNGG	5 cut(s)	815, 1010, 1282, 2140, 2146
Bse118I	RCCGGY	2 cut(s)	719, 1229
Bse1I	ACTGG	7 cut(s)	475, 809, 903, 1056, 1106, 1326, 1503
Bse3DI	GCAATG	3 cut(s)	18, 1817, 1956
BseBI	CCWGG	7 cut(s)	116, 341, 778, 1563, 1593, 1899, 2112
BseCI	ATCGAT	1 cut(s)	2153
BseDI	CCNNGG	8 cut(s)	115, 340, 676, 777, 1034, 1093, 1332, 1466
BseGI	GGATG	8 cut(s)	130, 310, 524, 864, 1186, 1428, 1566, 2149
BseLI	CCNNNNNNNGG	5 cut(s)	815, 1010, 1282, 2140, 2146
BseMI	GCAATG	3 cut(s)	18, 1817, 1956
BseMII	CTCAG	4 cut(s)	311, 339, 999, 1689
BseNI	ACTGG	7 cut(s)	475, 809, 903, 1056, 1106, 1326, 1503
BseRI	GAGGAG	3 cut(s)	49, 634, 2314
BseSI	GKGCMC	1 cut(s)	2006
BsgI	GTGCAG	1 cut(s)	1572
Bsh1236I	CGCG	3 cut(s)	661, 1468, 1790
Bsh1285I	CGRYCG	1 cut(s)	368
BshFI	GGCC	8 cut(s)	19, 481, 675, 863, 1229, 1271, 1633, 1725
BshNI	GGYRCC	2 cut(s)	1052, 1328
BshTI	ACCGGT	1 cut(s)	719
BshVI	ATCGAT	1 cut(s)	2153
BsiEI	CGRYCG	1 cut(s)	368
BsiHKAI	GWGCWC	1 cut(s)	598
BsiSI	CCGG	5 cut(s)	720, 1004, 1230, 1713, 2141
BslFI	GGGAC	7 cut(s)	396, 618, 1131, 1298, 1557, 2007, 2039
BslI	CCNNNNNNNGG	5 cut(s)	815, 1010, 1282, 2140, 2146
BsmAI	GTCTC	4 cut(s)	328, 548, 810, 1467
BsmFI	GGGAC	7 cut(s)	396, 618, 1131, 1298, 1557, 2007, 2039
BsnI	GGCC	8 cut(s)	19, 481, 675, 863, 1229, 1271, 1633, 1725
Bso31I	GGTCTC	1 cut(s)	810
Bsp1286I	GDGCHC	2 cut(s)	598, 2006
Bsp1720I	GCTNAGC	2 cut(s)	306, 985
Bsp19I	CCATGG	1 cut(s)	676
BspACI	CCGC	8 cut(s)	27, 659, 699, 989, 1178, 1466, 1468, 1788
BspANI	GGCC	8 cut(s)	19, 481, 675, 863, 1229, 1271, 1633, 1725
BspCNI	CTCAG	4 cut(s)	310, 338, 998, 1688
BspDI	ATCGAT	1 cut(s)	2153
BspFNI	CGCG	3 cut(s)	661, 1468, 1790
BspHI	TCATGA	2 cut(s)	1779, 2184
BspMAI	CTGCAG	3 cut(s)	625, 1762, 2226
BspMI	ACCTGC	1 cut(s)	2215
BspPI	GGATC	9 cut(s)	127, 772, 902, 1235, 1379, 1889, 1902, 2064, 2077
BspQI	GCTCTTC	1 cut(s)	2198
BspT107I	GGYRCC	2 cut(s)	1052, 1328
BspTI	CTTAAG	1 cut(s)	1571
BspTNI	GGTCTC	1 cut(s)	810
BsrDI	GCAATG	3 cut(s)	18, 1817, 1956
BsrFI	RCCGGY	2 cut(s)	719, 1229
BsrI	ACTGG	7 cut(s)	475, 809, 903, 1056, 1106, 1326, 1503
BssAI	RCCGGY	2 cut(s)	719, 1229
BssECI	CCNNGG	8 cut(s)	115, 340, 676, 777, 1034, 1093, 1332, 1466
BssNAI	GTATAC	2 cut(s)	112, 1909
BssNI	GRCGYC	2 cut(s)	1053, 1329
BssSI	CACGAG	1 cut(s)	1797
BssT1I	CCWWGG	1 cut(s)	676
Bst1107I	GTATAC	2 cut(s)	112, 1909
Bst2BI	CACGAG	1 cut(s)	1797
Bst2UI	CCWGG	7 cut(s)	116, 341, 778, 1563, 1593, 1899, 2112
Bst4CI	ACNGT	9 cut(s)	440, 875, 932, 1018, 1035, 1048, 1886, 2053, 2092
Bst6I	CTCTTC	3 cut(s)	282, 2193, 2198
BstACI	GRCGYC	2 cut(s)	1053, 1329
BstAFI	CTTAAG	1 cut(s)	1571
BstAPI	GCANNNNNTGC	1 cut(s)	1196
BstBAI	YACGTR	1 cut(s)	1995
BstC8I	GCNNGC	9 cut(s)	727, 1231, 1235, 1273, 1277, 1314, 1327, 1470, 1485
BstDEI	CTNAG	6 cut(s)	297, 306, 325, 985, 1458, 1675
BstDSI	CCRYGG	4 cut(s)	676, 1034, 1332, 1466
BstF5I	GGATG	8 cut(s)	130, 310, 524, 864, 1186, 1428, 1566, 2149
BstFNI	CGCG	3 cut(s)	661, 1468, 1790
BstH2I	RGCGCY	2 cut(s)	1056, 1332
BstHHI	GCGC	5 cut(s)	476, 1055, 1331, 1865, 1949
BstMAI	GTCTC	4 cut(s)	328, 548, 810, 1467
BstMCI	CGRYCG	1 cut(s)	368
BstNI	CCWGG	7 cut(s)	116, 341, 778, 1563, 1593, 1899, 2112
BstNSI	RCATGY	2 cut(s)	1394, 1874
BstSCI	CCNGG	8 cut(s)	114, 339, 776, 1561, 1591, 1897, 2110, 2139
BstSFI	CTRYAG	3 cut(s)	621, 1758, 2222
BstSLI	GKGCMC	1 cut(s)	2006
BstSNI	TACGTA	1 cut(s)	1995
BstUI	CGCG	3 cut(s)	661, 1468, 1790
BstX2I	RGATCY	4 cut(s)	907, 1263, 1894, 2069
BstXI	CCANNNNNNTGG	2 cut(s)	531, 1063
BstYI	RGATCY	4 cut(s)	907, 1263, 1894, 2069
BstZ17I	GTATAC	2 cut(s)	112, 1909
Bsu15I	ATCGAT	1 cut(s)	2153
BsuI	GTATCC	1 cut(s)	1984
BsuRI	GGCC	8 cut(s)	19, 481, 675, 863, 1229, 1271, 1633, 1725
BsuTUI	ATCGAT	1 cut(s)	2153
BtgI	CCRYGG	4 cut(s)	676, 1034, 1332, 1466
BtsCI	GGATG	8 cut(s)	130, 310, 524, 864, 1186, 1428, 1566, 2149
BtsI	GCAGTG	2 cut(s)	571, 1584
BveI	ACCTGC	1 cut(s)	2215
Cac8I	GCNNGC	9 cut(s)	727, 1231, 1235, 1273, 1277, 1314, 1327, 1470, 1485
CaiI	CAGNNNCTG	2 cut(s)	563, 629
CciI	TCATGA	2 cut(s)	1779, 2184
CfoI	GCGC	5 cut(s)	476, 1055, 1331, 1865, 1949
Cfr10I	RCCGGY	2 cut(s)	719, 1229
Cfr13I	GGNCC	5 cut(s)	515, 632, 1001, 1544, 1631
Cfr42I	CCGCGG	1 cut(s)	1469
ClaI	ATCGAT	1 cut(s)	2153
Csp6I	GTAC	4 cut(s)	68, 1014, 1059, 2216
CspAI	ACCGGT	1 cut(s)	719
CviQI	GTAC	4 cut(s)	68, 1014, 1059, 2216
DdeI	CTNAG	6 cut(s)	297, 306, 325, 985, 1458, 1675
DinI	GGCGCC	2 cut(s)	1054, 1330
DraIII	CACNNNGTG	2 cut(s)	902, 2296
EaeI	YGGCCR	3 cut(s)	479, 673, 861
Eam1104I	CTCTTC	3 cut(s)	282, 2193, 2198
EarI	CTCTTC	3 cut(s)	282, 2193, 2198
Eco105I	TACGTA	1 cut(s)	1995
Eco130I	CCWWGG	1 cut(s)	676
Eco31I	GGTCTC	1 cut(s)	810
Eco32I	GATATC	1 cut(s)	1582
Eco47I	GGWCC	4 cut(s)	515, 632, 1001, 1544
Eco57I	CTGAAG	2 cut(s)	907, 1029
EcoO109I	RGGNCCY	1 cut(s)	1544
EcoRI	GAATTC	1 cut(s)	1662
EcoRII	CCWGG	7 cut(s)	114, 339, 776, 1561, 1591, 1897, 2110
EcoRV	GATATC	1 cut(s)	1582
EcoT14I	CCWWGG	1 cut(s)	676
EcoT22I	ATGCAT	1 cut(s)	1201
EgeI	GGCGCC	2 cut(s)	1054, 1330
EheI	GGCGCC	2 cut(s)	1054, 1330
ErhI	CCWWGG	1 cut(s)	676
FaqI	GGGAC	7 cut(s)	396, 618, 1131, 1298, 1557, 2007, 2039
FauI	CCCGC	2 cut(s)	982, 1461
FauNDI	CATATG	1 cut(s)	103
FblI	GTMKAC	2 cut(s)	111, 1908
FokI	GGATG	8 cut(s)	117, 297, 511, 851, 1193, 1415, 1553, 2156
FspBI	CTAG	4 cut(s)	65, 278, 1134, 2085
FspI	TGCGCA	1 cut(s)	1948
GlaI	GCGC	5 cut(s)	475, 1054, 1330, 1864, 1948
GsuI	CTGGAG	3 cut(s)	792, 1614, 2094
HaeII	RGCGCY	2 cut(s)	1056, 1332
HaeIII	GGCC	8 cut(s)	19, 481, 675, 863, 1229, 1271, 1633, 1725
HapII	CCGG	5 cut(s)	720, 1004, 1230, 1713, 2141
HhaI	GCGC	5 cut(s)	476, 1055, 1331, 1865, 1949
Hin1I	GRCGYC	2 cut(s)	1053, 1329
Hin6I	GCGC	5 cut(s)	474, 1053, 1329, 1863, 1947
HinP1I	GCGC	5 cut(s)	474, 1053, 1329, 1863, 1947
HincII	GTYRAC	2 cut(s)	696, 1876
HindII	GTYRAC	2 cut(s)	696, 1876
HindIII	AAGCTT	3 cut(s)	584, 1123, 2319
HpaI	GTTAAC	1 cut(s)	1876
HpaII	CCGG	5 cut(s)	720, 1004, 1230, 1713, 2141
Hpy166II	GTNNAC	7 cut(s)	112, 696, 1300, 1703, 1876, 1909, 2288
Hpy188I	TCNGA	7 cut(s)	148, 300, 565, 1080, 1384, 1987, 2281
Hpy188III	TCNNGA	6 cut(s)	334, 421, 1021, 1780, 2067, 2185
Hpy8I	GTNNAC	7 cut(s)	112, 696, 1300, 1703, 1876, 1909, 2288
Hpy99I	CGWCG	1 cut(s)	1427
HpyCH4III	ACNGT	9 cut(s)	440, 875, 932, 1018, 1035, 1048, 1886, 2053, 2092
HpyCH4IV	ACGT	1 cut(s)	1994
HpyF3I	CTNAG	6 cut(s)	297, 306, 325, 985, 1458, 1675
HpySE526I	ACGT	1 cut(s)	1994
Hsp92I	GRCGYC	2 cut(s)	1053, 1329
HspAI	GCGC	5 cut(s)	474, 1053, 1329, 1863, 1947
KasI	GGCGCC	2 cut(s)	1052, 1328
KflI	GGGWCCC	1 cut(s)	1544
KroI	GCCGGC	1 cut(s)	1229
KroNI	GCCGGC	1 cut(s)	1231
KspAI	GTTAAC	1 cut(s)	1876
KspI	CCGCGG	1 cut(s)	1469
LguI	GCTCTTC	1 cut(s)	2198
LmnI	GCTCC	2 cut(s)	593, 686
LweI	GCATC	5 cut(s)	319, 1171, 1208, 1437, 1575
MaeI	CTAG	4 cut(s)	65, 278, 1134, 2085
MaeII	ACGT	1 cut(s)	1994
MaeIII	GTNAC	7 cut(s)	166, 662, 715, 926, 1087, 1856, 2080
MboII	GAAGA	9 cut(s)	293, 296, 299, 357, 436, 455, 913, 2210, 2215
MfeI	CAATTG	2 cut(s)	939, 2307
MflI	RGATCY	4 cut(s)	907, 1263, 1894, 2069
MhlI	GDGCHC	2 cut(s)	598, 2006
MlsI	TGGCCA	3 cut(s)	481, 675, 863
MluNI	TGGCCA	3 cut(s)	481, 675, 863
Mly113I	GGCGCC	2 cut(s)	1053, 1329
MlyI	GAGTC	2 cut(s)	1762, 2311
Mox20I	TGGCCA	3 cut(s)	481, 675, 863
Mph1103I	ATGCAT	1 cut(s)	1201
MroNI	GCCGGC	1 cut(s)	1229
MscI	TGGCCA	3 cut(s)	481, 675, 863
MseI	TTAA	8 cut(s)	960, 1362, 1572, 1731, 1767, 1875, 2211, 2229
MslI	CAYNNNNRTG	5 cut(s)	258, 529, 873, 974, 1691
Msp20I	TGGCCA	3 cut(s)	481, 675, 863
MspA1I	CMGCKG	4 cut(s)	626, 701, 989, 1468
MspCI	CTTAAG	1 cut(s)	1571
MspI	CCGG	5 cut(s)	720, 1004, 1230, 1713, 2141
MspR9I	CCNGG	8 cut(s)	116, 341, 778, 1563, 1593, 1899, 2112, 2141
MunI	CAATTG	2 cut(s)	939, 2307
MvaI	CCWGG	7 cut(s)	116, 341, 778, 1563, 1593, 1899, 2112
MvnI	CGCG	3 cut(s)	661, 1468, 1790
NaeI	GCCGGC	1 cut(s)	1231
NarI	GGCGCC	2 cut(s)	1053, 1329
NciI	CCSGG	1 cut(s)	2141
NcoI	CCATGG	1 cut(s)	676
NdeI	CATATG	1 cut(s)	103
NgoMIV	GCCGGC	1 cut(s)	1229
NmeAIII	GCCGAG	1 cut(s)	1074
NmuCI	GTSAC	5 cut(s)	166, 662, 926, 1087, 2080
NsbI	TGCGCA	1 cut(s)	1948
NsiI	ATGCAT	1 cut(s)	1201
NspI	RCATGY	2 cut(s)	1394, 1874
PagI	TCATGA	2 cut(s)	1779, 2184
PciI	ACATGT	2 cut(s)	1390, 1870
PciSI	GCTCTTC	1 cut(s)	2198
PdiI	GCCGGC	1 cut(s)	1231
PfeI	GAWTC	8 cut(s)	227, 446, 485, 520, 540, 560, 653, 1342
PflMI	CCANNNNNTGG	1 cut(s)	815
PfoI	TCCNGGA	2 cut(s)	1897, 2110
PinAI	ACCGGT	1 cut(s)	719
Ple19I	CGATCG	1 cut(s)	368
PleI	GAGTC	2 cut(s)	1761, 2310
PluTI	GGCGCC	2 cut(s)	1056, 1332
PpsI	GAGTC	2 cut(s)	1761, 2310
Ppu21I	YACGTR	1 cut(s)	1995
PpuMI	RGGWCCY	1 cut(s)	1544
PscI	ACATGT	2 cut(s)	1390, 1870
Psp5II	RGGWCCY	1 cut(s)	1544
Psp6I	CCWGG	7 cut(s)	114, 339, 776, 1561, 1591, 1897, 2110
PspGI	CCWGG	7 cut(s)	114, 339, 776, 1561, 1591, 1897, 2110
PspPI	GGNCC	5 cut(s)	515, 632, 1001, 1544, 1631
PspPPI	RGGWCCY	1 cut(s)	1544
PstI	CTGCAG	3 cut(s)	625, 1762, 2226
PstNI	CAGNNNCTG	2 cut(s)	563, 629
PsuI	RGATCY	4 cut(s)	907, 1263, 1894, 2069
PvuI	CGATCG	1 cut(s)	368
PvuII	CAGCTG	1 cut(s)	626
RsaI	GTAC	4 cut(s)	69, 1015, 1060, 2217
RsaNI	GTAC	4 cut(s)	68, 1014, 1059, 2216
RseI	CAYNNNNRTG	5 cut(s)	258, 529, 873, 974, 1691
SacII	CCGCGG	1 cut(s)	1469
SapI	GCTCTTC	1 cut(s)	2198
SaqAI	TTAA	8 cut(s)	960, 1362, 1572, 1731, 1767, 1875, 2211, 2229
Sau96I	GGNCC	5 cut(s)	515, 632, 1001, 1544, 1631
SbfI	CCTGCAGG	1 cut(s)	2226
ScaI	AGTACT	2 cut(s)	69, 1060
SchI	GAGTC	2 cut(s)	1762, 2311
ScrFI	CCNGG	8 cut(s)	116, 341, 778, 1563, 1593, 1899, 2112, 2141
SdaI	CCTGCAGG	1 cut(s)	2226
SduI	GDGCHC	2 cut(s)	598, 2006
SfaNI	GCATC	5 cut(s)	319, 1171, 1208, 1437, 1575
SfcI	CTRYAG	3 cut(s)	621, 1758, 2222
SfoI	GGCGCC	2 cut(s)	1054, 1330
Sfr303I	CCGCGG	1 cut(s)	1469
SgrBI	CCGCGG	1 cut(s)	1469
SinI	GGWCC	4 cut(s)	515, 632, 1001, 1544
SmiMI	CAYNNNNRTG	5 cut(s)	258, 529, 873, 974, 1691
SmlI	CTYRAG	4 cut(s)	386, 1112, 1571, 2075
SmoI	CTYRAG	4 cut(s)	386, 1112, 1571, 2075
SnaBI	TACGTA	1 cut(s)	1995
Sse8387I	CCTGCAGG	1 cut(s)	2226
SsiI	CCGC	8 cut(s)	27, 659, 699, 989, 1178, 1466, 1468, 1788
SspDI	GGCGCC	2 cut(s)	1052, 1328
SspMI	CTAG	4 cut(s)	65, 278, 1134, 2085
StyD4I	CCNGG	8 cut(s)	114, 339, 776, 1561, 1591, 1897, 2110, 2139
StyI	CCWWGG	1 cut(s)	676
TaaI	ACNGT	9 cut(s)	440, 875, 932, 1018, 1035, 1048, 1886, 2053, 2092
TaiI	ACGT	1 cut(s)	1997
TaqI	TCGA	4 cut(s)	364, 651, 2153, 2163
TaqII	GACCGA	1 cut(s)	532
TatI	WGTACW	2 cut(s)	67, 1058
TauI	GCSGC	1 cut(s)	661
TfiI	GAWTC	8 cut(s)	227, 446, 485, 520, 540, 560, 653, 1342
Tru1I	TTAA	8 cut(s)	960, 1362, 1572, 1731, 1767, 1875, 2211, 2229
Tru9I	TTAA	8 cut(s)	960, 1362, 1572, 1731, 1767, 1875, 2211, 2229
TseFI	GTSAC	5 cut(s)	166, 662, 926, 1087, 2080
Tsp45I	GTSAC	5 cut(s)	166, 662, 926, 1087, 2080
TspDTI	ATGAA	8 cut(s)	228, 519, 821, 941, 1510, 1524, 2173, 2201
TspGWI	ACGGA	2 cut(s)	880, 1508
Van91I	CCANNNNNTGG	1 cut(s)	815
Vha464I	CTTAAG	1 cut(s)	1571
VpaK11BI	GGWCC	4 cut(s)	515, 632, 1001, 1544
XapI	RAATTY	4 cut(s)	566, 994, 1154, 1662
XceI	RCATGY	2 cut(s)	1394, 1874
XcmI	CCANNNNNNNNNTGG	2 cut(s)	188, 2141
XmiI	GTMKAC	2 cut(s)	111, 1908
XspI	CTAG	4 cut(s)	65, 278, 1134, 2085
ZrmI	AGTACT	2 cut(s)	69, 1060
Zsp2I	ATGCAT	1 cut(s)	1201

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.