Rh2BG524500

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0007593
Physical Location & Seq
Forward (+)
73451290 .. 73452008
719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG524500.1

Sequence Viewer

Length: 402 bp
ATGGATAGGGAGTTCCCTACTTATATCACTCTTGGCAACTGGAAACACTTCAAGGGACAAAGCTTATCAGCTGTGGCCTTGCCAAGCAAGAGGTTCTATCCACTTACAAGTGCTGCAGATGCTAAAGCAGCTAATACATCTATTCAAGAAGCTCTGCTGTGCAAGGCTGGAACGCTTGATCCAGAAAAGGTTAAGGGAAAGATATTGGCCTGCCTTCGAGGGGACAATGCAAGAGTGGACAAGGGTGAACAAGCCTTACTTGCTGGTGCTGTGGGGATGATTCTTGCTAACAGTGAAGTTAACGGGAATGAAATTATCTCCGATCCACATGTCCTCCCTGCTTCACATATCAATTTCACCGATGGCTCCCTTGTCTTTGCTTACATCAACTCAACCAAGTAA

Protein Analysis

133

Amino Acids

14.22

Weight (kDa)

7.78

Isoelectric Point (pI)

28.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PA PF02225 51 - 123 8.6e-13 PA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 173, 317
AfiI CCNNNNNNNGG 1 cut(s) 220
AflIII ACRYGT 1 cut(s) 328
AgsI TTSAA 2 cut(s) 52, 146
AloI GAACNNNNNNTCC 3 cut(s) 28, 163, 195
AluBI AGCT 4 cut(s) 63, 71, 131, 152
AluI AGCT 4 cut(s) 63, 71, 131, 152
AlwI GGATC 2 cut(s) 173, 317
AoxI GGCC 2 cut(s) 75, 207
ApeKI GCWGC 2 cut(s) 113, 128
Asp700I GAANNNNTTC 1 cut(s) 47
AsuHPI GGTGA 2 cut(s) 257, 349
BbvI GCAGC 2 cut(s) 100, 140
BccI CCATC 1 cut(s) 356
BfmI CTRYAG 1 cut(s) 114
BisI GCNGC 2 cut(s) 114, 129
BlsI GCNGC 2 cut(s) 115, 130
BmiI GGNNCC 1 cut(s) 367
BmsI GCATC 1 cut(s) 109
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 1 cut(s) 220
Bse1I ACTGG 1 cut(s) 44
BseGI GGATG 1 cut(s) 282
BseLI CCNNNNNNNGG 1 cut(s) 220
BseNI ACTGG 1 cut(s) 44
BseXI GCAGC 2 cut(s) 100, 140
BshFI GGCC 2 cut(s) 77, 209
BslFI GGGAC 2 cut(s) 69, 236
BslI CCNNNNNNNGG 1 cut(s) 220
BsmFI GGGAC 2 cut(s) 69, 236
BsnI GGCC 2 cut(s) 77, 209
Bsp143I GATC 2 cut(s) 178, 322
BspANI GGCC 2 cut(s) 77, 209
BspLI GGNNCC 1 cut(s) 367
BspMAI CTGCAG 1 cut(s) 118
BspPI GGATC 2 cut(s) 173, 317
BsrI ACTGG 1 cut(s) 44
BssMI GATC 2 cut(s) 178, 322
Bst4CI ACNGT 1 cut(s) 293
BstC8I GCNNGC 1 cut(s) 211
BstF5I GGATG 1 cut(s) 282
BstKTI GATC 2 cut(s) 181, 325
BstMBI GATC 2 cut(s) 178, 322
BstMWI GCNNNNNNNGC 3 cut(s) 119, 128, 260
BstNSI RCATGY 1 cut(s) 332
BstSFI CTRYAG 1 cut(s) 114
BstV1I GCAGC 2 cut(s) 100, 140
BsuRI GGCC 2 cut(s) 77, 209
BtsCI GGATG 1 cut(s) 282
BtsIMutI CAGTG 1 cut(s) 298
Cac8I GCNNGC 1 cut(s) 211
CviAII CATG 1 cut(s) 329
CviJI RGCY 9 cut(s) 63, 71, 77, 131, 152, 167, 209, 254, 366
CviKI_1 RGCY 9 cut(s) 63, 71, 77, 131, 152, 167, 209, 254, 366
DpnI GATC 2 cut(s) 180, 324
DpnII GATC 2 cut(s) 178, 322
FaeI CATG 1 cut(s) 332
FaiI YATR 3 cut(s) 24, 330, 348
FalI AAGNNNNNCTT 2 cut(s) 243, 275
FaqI GGGAC 2 cut(s) 69, 236
FatI CATG 1 cut(s) 328
Fnu4HI GCNGC 2 cut(s) 114, 129
FokI GGATG 1 cut(s) 289
Fsp4HI GCNGC 2 cut(s) 114, 129
GluI GCNGC 2 cut(s) 114, 129
HaeIII GGCC 2 cut(s) 77, 209
Hin1II CATG 1 cut(s) 332
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HindIII AAGCTT 1 cut(s) 61
HinfI GANTC 1 cut(s) 280
HpaI GTTAAC 1 cut(s) 301
HphI GGTGA 2 cut(s) 257, 349
Hpy166II GTNNAC 3 cut(s) 238, 248, 301
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 2 cut(s) 146, 182
Hpy8I GTNNAC 3 cut(s) 238, 248, 301
HpyAV CCTTC 1 cut(s) 224
HpyCH4III ACNGT 1 cut(s) 293
HpyCH4V TGCA 3 cut(s) 116, 162, 230
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 128, 260
Hsp92II CATG 1 cut(s) 332
KspAI GTTAAC 1 cut(s) 301
Kzo9I GATC 2 cut(s) 178, 322
LmnI GCTCC 1 cut(s) 371
LpnPI CCDG 6 cut(s) 25, 153, 195, 223, 249, 351
Lsp1109I GCAGC 2 cut(s) 100, 140
LweI GCATC 1 cut(s) 109
MalI GATC 2 cut(s) 180, 324
MboI GATC 2 cut(s) 178, 322
MluCI AATT 2 cut(s) 312, 352
MnlI CCTC 3 cut(s) 84, 212, 344
MroXI GAANNNNTTC 1 cut(s) 47
MseI TTAA 2 cut(s) 192, 300
MspA1I CMGCKG 1 cut(s) 71
MwoI GCNNNNNNNGC 3 cut(s) 119, 128, 260
NdeII GATC 2 cut(s) 178, 322
NlaIII CATG 1 cut(s) 332
NlaIV GGNNCC 1 cut(s) 367
NspI RCATGY 1 cut(s) 332
PciI ACATGT 1 cut(s) 328
PdmI GAANNNNTTC 1 cut(s) 47
PfeI GAWTC 1 cut(s) 280
PkrI GCNGC 2 cut(s) 115, 130
PscI ACATGT 1 cut(s) 328
PspN4I GGNNCC 1 cut(s) 367
PsrI GAACNNNNNNTAC 2 cut(s) 240, 272
PstI CTGCAG 1 cut(s) 118
PvuII CAGCTG 1 cut(s) 71
SaqAI TTAA 2 cut(s) 192, 300
SatI GCNGC 2 cut(s) 114, 129
Sau3AI GATC 2 cut(s) 178, 322
SetI ASST 6 cut(s) 65, 73, 95, 133, 154, 192
SfaNI GCATC 1 cut(s) 109
SfcI CTRYAG 1 cut(s) 114
Sse9I AATT 2 cut(s) 312, 352
TaaI ACNGT 1 cut(s) 293
TaqI TCGA 1 cut(s) 217
TasI AATT 2 cut(s) 312, 352
TfiI GAWTC 1 cut(s) 280
Tru1I TTAA 2 cut(s) 192, 300
Tru9I TTAA 2 cut(s) 192, 300
TscAI CASTG 1 cut(s) 298
TseI GCWGC 2 cut(s) 113, 128
TspDTI ATGAA 1 cut(s) 324
TspRI CASTG 1 cut(s) 298
XceI RCATGY 1 cut(s) 332
XmnI GAANNNNTTC 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.