Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0007593

Physical Location & Seq

Genomic Coordinates Reverse (-)

10478903 .. 10484667

Gene Structure 5765 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1120300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2322 bp

ATGAGGCCGCCAACCACAGCCCTTTGCCTCCTCTCCTTCCTTCTCTCCTCTCTAGTACTCCATACTCCCACCTTTGCCATAAAAAAGTCGTATGTGGTGTACTTGGGATCACATTCGCATCCCCCAAACTTATCGGAACTTGAACTAAATCAAGTCACAGACAATCACTATGAGTTTCTTGGCTCCTTCTTGGGCAGCCATGAAGTTGCAAAAGAATCCCTCTTTTACTCTTACACAAGGCACATCAATGGCTTTGCTGCCACCCTAGAAGAAGAAGAGGCAGCTCAGATTGCAAGGCATCCAAGGGTAGTCTCATTATTCTTGAACCAGGGAAGAAAATTACACACAACTCGATCGTGGGATTTCTTGGGACTTGAGCAAGAGGGTGTTGTTCCACCCAATTCAATCTGGAAGAAGGCAAGATATGGTGAAGATTCGATCATAGGAAACCTTGATACTGGCGCATGGCCAGAATCCAAGAGCTTTAGTGATGAGGGGTATGGACCGATTCCATCCAAGTGGAAAGGAATCTGTCAAAATCAGACAGATCCAAAATTTCACTGCAACAGGAAGCTAATTGGAGCAAGATACTTCAACAAGGGCTTTGCTGCAGTTGCTGGCCCGCTCAACTCCTCCTTCGATTCGCCGCGCGACAACGAAGGCCATGGCTCCCATACCTTGTCAACAGCTGGTGGAAATTTCGTAACCGGTGCAAGCGCGTTCGGCTTTGGTAATGGAACAGCAAAGGGTGGATCACCAAAAGCCAGGGTTGCAGCCTATAAGGTCTGCTGGCCTCCAATGGAAGGAGGTGAGTGCTTTGATGCAGATATATTGGCTGCATTTGATGTGGCCATCGATGACGGCGTTGATGTGTTGTCCATTTCACTGGGTGGAGATCCCACCACATTCTTCAATGACAGTGTTTCAATTGGTGCTTTCCATGCTGTTAAGCGTGGCATTGTGGTGGTTTGCTCAGCGGGGAATTCTGGTCCGGCTGAAGGTACTGTCTCCAATATATCGCCATGGCAGATCACGGTCGGCGCTAGTACTATGGATAGGGAGTTTCCTAGTTATGTCACCCTTGGAAACTGGAAGCACTTTAAGGGACAAAGCTTATCGACTGTTGCTTTGCGGAGTAAGAAAAAGAAATTCTACCAACTTATTAGTGCTGCAGATGCTAAAGCAGCTAATGCATCAGCTCAAGAAGCTCTGCTATGCAAGCCCAACACGCTTGATCCTAAAAAAGTGAAGGGAAAGATCTTGGCCTGTCTTCGAGGGGACAATGCAAGAGTGGACAAGGGTGAGCAAGCCTTACTGGCTGGCGCAGTGGGAATGATTCTTGCTAACAATGAGCTTACCGGGAATGAAATTATTGCTGATCCACATGTTCTCCCTGCTTCGCATATCAATTTCACCGACGGCAACCTTGTCTTTACTTATATTAATTCAACTAAGTCTCCGCGGGCTTACATAAAGCGTGCAATAACGGAACTGGGAACGAAGCCATCTCCATTCATGGCAGCATTTTCATCGAGGGGACCCAACACCATTACACCAGGCATCCTTAAGCCTGATATCACTGCACCTGGAGTGAGTGTCATAGCAGCCTACACAGAAGCACAAGGGCCAACAAATCAAATGTTTGACAAACGGCGAATTCCATTCAACTCAGTGTCAGGCACATCAATGTCATGTCCACATATTTCCGGCATTTGTGGCCTTCTTAAAACCCTCTATCCTCATTGGAGTCCTGCAGCCATTAAGTCTGCAATCATGACCACCGCGGTAACACGAGATAACAGCATGGAACCATTGCTCAATGCTTCTTTCTATGAGGCAACACCATTTAGTTACGGCGCAGGACATGTTAACCCAAACAGTGCTATGGATCCTGGATTGGTTTACGACTTATCCCTTAACGATTACTTGAACTTCCTGTGCGCAATTGGCTACAATGAAACACAAATTGAAATGTTTTCAGAGGAGATGTATATGTGCTCCAAACCAGCTATAAGTCTCACTAGCCTCAACTACCCCTCAATCACTGTCCCTAAAATCTCTGGATCCCTTGAGGTGACTAGAACAGTGAAAAATGTTGGCTCTCCAGGAACATATAAGGCGCGTATCCAAAATCCAGATGGTATATCAGTTTCTGTTGAGCCAAAGGAGTTGAAGTTCAAGAAAATTGGTGAAGAGAAAAGCTTTAAGGTACTCCTGCAGGTTAGAGAAGCAAAACAAGATAAAAACTACGTGTTTGGGAAGTTGATATGGTCAGATGGTAAACACTATGTGAGGAGTCCAATTGTTGTCAAGGCTGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0016787 GO:0017171 GO:0019538 GO:0022622 GO:0032501 GO:0032502 GO:0042221 GO:0043170 GO:0044238 GO:0048364 GO:0048527 GO:0048528 GO:0048731 GO:0048856 GO:0050896 GO:0070011 GO:0071704 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140096 GO:1901564 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

774

Amino Acids

83.6

Weight (kDa)

8.66

Isoelectric Point (pI)

31.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	30 - 115	3.9e-16	Peptidase inhibitor I9
Peptidase_S8	PF00082	147 - 621	9.1e-38	Subtilase family
PA	PF02225	403 - 475	3.3e-12	PA domain
fn3_6	PF17766	676 - 770	4.8e-32	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007593)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04160
fragaria_vesca	FvH4_6g40300
malus_domestica	MD09G1132200.v1.1 MD09G1132600.v1.1 MD17G1120300.v1.1
prunus_persica	Prupe.3G147300_v2.0.a1 Prupe.3G193800_v2.0.a1
pyrus_communis	pycom09g05410 pycom17g11090
rosa_chinensis	RchiOBHm_Chr2g0155491
rosa_laevigata	RLG00000020840
rosa_multiflora	Rmu_sc0003439.1_g000012
rosa_roxburghii	Rroxscaffold_2G00093670
rosa_rugosa	Rorug02G0447200 Rorug02G0447200
rosa_samantha	Rh2AG513600 Rh2AG513700 Rh2BG524400 Rh2BG524500 Rh2BG524600 Rh2CG498500 Rh2CG498600 Rh2DG533600
rosa_wichuraiana	Rw2G042220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1942
Acc36I	ACCTGC	1 cut(s)	2209
AccBSI	CCGCTC	1 cut(s)	625
AccII	CGCG	6 cut(s)	649, 651, 719, 1462, 1784, 2122
AciI	CCGC	9 cut(s)	8, 623, 647, 977, 1132, 1460, 1462, 1782, 1784
AcoI	YGGCCR	2 cut(s)	467, 849
AcsI	RAATTY	5 cut(s)	554, 697, 982, 1148, 1656
AcuI	CTGAAG	1 cut(s)	1017
AdeI	CACNNNGTG	2 cut(s)	890, 2290
AfaI	GTAC	5 cut(s)	57, 101, 1003, 1048, 2211
AfiI	CCNNNNNNNGG	2 cut(s)	803, 998
AflII	CTTAAG	1 cut(s)	1565
AflIII	ACRYGT	3 cut(s)	1384, 1864, 2250
AgeI	ACCGGT	1 cut(s)	707
AjnI	CCWGG	6 cut(s)	327, 764, 1555, 1585, 1891, 2104
AloI	GAACNNNNNNTCC	2 cut(s)	2159, 2191
Alw21I	GWGCWC	1 cut(s)	2000
Alw26I	GTCTC	4 cut(s)	316, 1012, 1461, 2021
AlwNI	CAGNNNCTG	2 cut(s)	617, 2153
AoxI	GGCC	9 cut(s)	5, 467, 619, 661, 791, 849, 1263, 1625, 1717
ApoI	RAATTY	5 cut(s)	554, 697, 982, 1148, 1656
ArsI	GACNNNNNNTTYG	2 cut(s)	1111, 1143
AseI	ATTAAT	1 cut(s)	1443
AsiGI	ACCGGT	1 cut(s)	707
AspLEI	GCGC	8 cut(s)	464, 651, 719, 1043, 1325, 1859, 1943, 2122
AspS9I	GGNCC	5 cut(s)	503, 620, 989, 1538, 1625
AsuC2I	CCSGG	1 cut(s)	1360
AsuHPI	GGTGA	8 cut(s)	440, 747, 821, 1069, 1313, 1405, 2086, 2201
AvaII	GGWCC	3 cut(s)	503, 989, 1538
BalI	TGGCCA	2 cut(s)	469, 851
BamHI	GGATCC	2 cut(s)	1888, 2063
BauI	CACGAG	1 cut(s)	1791
BbsI	GAAGAC	1 cut(s)	1262
Bbv12I	GWGCWC	1 cut(s)	2000
BccI	CCATC	5 cut(s)	520, 860, 1513, 2132, 2270
BceAI	ACGGC	4 cut(s)	877, 1435, 1667, 1870
BcgI	CGANNNNNNTGC	2 cut(s)	1512, 1546
BciT130I	CCWGG	6 cut(s)	329, 766, 1557, 1587, 1893, 2106
BciVI	GTATCC	1 cut(s)	2135
BcnI	CCSGG	1 cut(s)	1360
BcoDI	GTCTC	4 cut(s)	316, 1012, 1461, 2021
BfaI	CTAG	6 cut(s)	53, 266, 1044, 1068, 2022, 2079
BfmI	CTRYAG	4 cut(s)	609, 1170, 1752, 2216
BfoI	RGCGCY	1 cut(s)	1044
BfrI	CTTAAG	1 cut(s)	1565
BfuAI	ACCTGC	1 cut(s)	2209
BfuI	GTATCC	1 cut(s)	2135
BglI	GCCNNNNNGGC	1 cut(s)	1316
BglII	AGATCT	1 cut(s)	1257
BlpI	GCTNAGC	1 cut(s)	973
BmcAI	AGTACT	2 cut(s)	57, 1048
Bme1390I	CCNGG	7 cut(s)	329, 766, 1360, 1557, 1587, 1893, 2106
Bme18I	GGWCC	3 cut(s)	503, 989, 1538
BmgT120I	GGNCC	5 cut(s)	503, 620, 989, 1538, 1625
BmiI	GGNNCC	7 cut(s)	184, 670, 1539, 1540, 1809, 1890, 2065
BmrFI	CCNGG	7 cut(s)	329, 766, 1360, 1557, 1587, 1893, 2106
BmrI	ACTGGG	2 cut(s)	896, 1502
BmsI	GCATC	6 cut(s)	127, 307, 811, 1165, 1202, 1569
BmuI	ACTGGG	2 cut(s)	896, 1502
BpiI	GAAGAC	1 cut(s)	1262
BpmI	CTGGAG	2 cut(s)	1608, 2088
Bpu1102I	GCTNAGC	1 cut(s)	973
BpuEI	CTTGAG	3 cut(s)	395, 1185, 2090
BpuMI	CCSGG	1 cut(s)	1360
Bsa29I	ATCGAT	1 cut(s)	855
BsaAI	YACGTR	1 cut(s)	2251
BsaJI	CCNNGG	8 cut(s)	302, 328, 664, 765, 1022, 1081, 1460, 1782
BsaWI	WCCGGW	1 cut(s)	707
BsaXI	ACNNNNNCTCC	8 cut(s)	1374, 1404, 1441, 1471, 1581, 1611, 2159, 2189
Bsc4I	CCNNNNNNNGG	2 cut(s)	803, 998
Bse118I	RCCGGY	1 cut(s)	707
Bse1I	ACTGG	5 cut(s)	463, 891, 1094, 1320, 1497
Bse3DI	GCAATG	1 cut(s)	1811
BseBI	CCWGG	6 cut(s)	329, 766, 1557, 1587, 1893, 2106
BseCI	ATCGAT	1 cut(s)	855
BseDI	CCNNGG	8 cut(s)	302, 328, 664, 765, 1022, 1081, 1460, 1782
BseGI	GGATG	4 cut(s)	118, 298, 512, 1560
BseLI	CCNNNNNNNGG	2 cut(s)	803, 998
BseMI	GCAATG	1 cut(s)	1811
BseMII	CTCAG	3 cut(s)	299, 987, 1683
BseNI	ACTGG	5 cut(s)	463, 891, 1094, 1320, 1497
BseRI	GAGGAG	5 cut(s)	20, 37, 622, 1997, 2308
BsgI	GTGCAG	1 cut(s)	1566
Bsh1236I	CGCG	6 cut(s)	649, 651, 719, 1462, 1784, 2122
Bsh1285I	CGRYCG	2 cut(s)	356, 1038
BshFI	GGCC	9 cut(s)	7, 469, 621, 663, 793, 851, 1265, 1627, 1719
BshTI	ACCGGT	1 cut(s)	707
BshVI	ATCGAT	1 cut(s)	855
BsiEI	CGRYCG	2 cut(s)	356, 1038
BsiHKAI	GWGCWC	1 cut(s)	2000
BsiSI	CCGG	4 cut(s)	708, 992, 1359, 1707
BslFI	GGGAC	5 cut(s)	384, 1119, 1292, 1551, 2033
BslI	CCNNNNNNNGG	2 cut(s)	803, 998
BsmAI	GTCTC	4 cut(s)	316, 1012, 1461, 2021
BsmFI	GGGAC	5 cut(s)	384, 1119, 1292, 1551, 2033
BsnI	GGCC	9 cut(s)	7, 469, 621, 663, 793, 851, 1265, 1627, 1719
Bsp1286I	GDGCHC	1 cut(s)	2000
Bsp1720I	GCTNAGC	1 cut(s)	973
Bsp19I	CCATGG	2 cut(s)	664, 1022
BspACI	CCGC	9 cut(s)	8, 623, 647, 977, 1132, 1460, 1462, 1782, 1784
BspANI	GGCC	9 cut(s)	7, 469, 621, 663, 793, 851, 1265, 1627, 1719
BspCNI	CTCAG	3 cut(s)	298, 986, 1682
BspDI	ATCGAT	1 cut(s)	855
BspFNI	CGCG	6 cut(s)	649, 651, 719, 1462, 1784, 2122
BspHI	TCATGA	1 cut(s)	1773
BspLI	GGNNCC	7 cut(s)	184, 670, 1539, 1540, 1809, 1890, 2065
BspMAI	CTGCAG	4 cut(s)	613, 1174, 1756, 2220
BspMI	ACCTGC	1 cut(s)	2209
BspTI	CTTAAG	1 cut(s)	1565
BsrBI	CCGCTC	1 cut(s)	625
BsrDI	GCAATG	1 cut(s)	1811
BsrFI	RCCGGY	1 cut(s)	707
BsrI	ACTGG	5 cut(s)	463, 891, 1094, 1320, 1497
BssAI	RCCGGY	1 cut(s)	707
BssECI	CCNNGG	8 cut(s)	302, 328, 664, 765, 1022, 1081, 1460, 1782
BssSI	CACGAG	1 cut(s)	1791
BssT1I	CCWWGG	4 cut(s)	302, 664, 1022, 1081
Bst2BI	CACGAG	1 cut(s)	1791
Bst2UI	CCWGG	6 cut(s)	329, 766, 1557, 1587, 1893, 2106
Bst4CI	ACNGT	7 cut(s)	920, 1006, 1036, 1123, 1880, 2047, 2086
Bst6I	CTCTTC	2 cut(s)	270, 2187
BstAFI	CTTAAG	1 cut(s)	1565
BstAPI	GCANNNNNTGC	1 cut(s)	1190
BstBAI	YACGTR	1 cut(s)	2251
BstC8I	GCNNGC	9 cut(s)	619, 623, 715, 791, 1220, 1308, 1321, 1464, 1479
BstDEI	CTNAG	4 cut(s)	285, 973, 1452, 1669
BstDSI	CCRYGG	4 cut(s)	664, 1022, 1460, 1782
BstF5I	GGATG	4 cut(s)	118, 298, 512, 1560
BstFNI	CGCG	6 cut(s)	649, 651, 719, 1462, 1784, 2122
BstH2I	RGCGCY	1 cut(s)	1044
BstHHI	GCGC	8 cut(s)	464, 651, 719, 1043, 1325, 1859, 1943, 2122
BstMAI	GTCTC	4 cut(s)	316, 1012, 1461, 2021
BstMCI	CGRYCG	2 cut(s)	356, 1038
BstNI	CCWGG	6 cut(s)	329, 766, 1557, 1587, 1893, 2106
BstNSI	RCATGY	2 cut(s)	1388, 1868
BstSCI	CCNGG	7 cut(s)	327, 764, 1358, 1555, 1585, 1891, 2104
BstSFI	CTRYAG	4 cut(s)	609, 1170, 1752, 2216
BstUI	CGCG	6 cut(s)	649, 651, 719, 1462, 1784, 2122
BstV2I	GAAGAC	1 cut(s)	1262
BstX2I	RGATCY	5 cut(s)	547, 895, 1257, 1888, 2063
BstXI	CCANNNNNNTGG	2 cut(s)	519, 886
BstYI	RGATCY	5 cut(s)	547, 895, 1257, 1888, 2063
Bsu15I	ATCGAT	1 cut(s)	855
BsuI	GTATCC	1 cut(s)	2135
BsuRI	GGCC	9 cut(s)	7, 469, 621, 663, 793, 851, 1265, 1627, 1719
BsuTUI	ATCGAT	1 cut(s)	855
BtgI	CCRYGG	4 cut(s)	664, 1022, 1460, 1782
BtsCI	GGATG	4 cut(s)	118, 298, 512, 1560
BtsI	GCAGTG	3 cut(s)	559, 1332, 1578
BtsIMutI	CAGTG	9 cut(s)	559, 884, 925, 1332, 1578, 1677, 1885, 2043, 2091
BveI	ACCTGC	1 cut(s)	2209
Cac8I	GCNNGC	9 cut(s)	619, 623, 715, 791, 1220, 1308, 1321, 1464, 1479
CaiI	CAGNNNCTG	2 cut(s)	617, 2153
CciI	TCATGA	1 cut(s)	1773
CfoI	GCGC	8 cut(s)	464, 651, 719, 1043, 1325, 1859, 1943, 2122
Cfr10I	RCCGGY	1 cut(s)	707
Cfr13I	GGNCC	5 cut(s)	503, 620, 989, 1538, 1625
Cfr42I	CCGCGG	2 cut(s)	1463, 1785
ClaI	ATCGAT	1 cut(s)	855
Csp6I	GTAC	5 cut(s)	56, 100, 1002, 1047, 2210
CspAI	ACCGGT	1 cut(s)	707
CviQI	GTAC	5 cut(s)	56, 100, 1002, 1047, 2210
DdeI	CTNAG	4 cut(s)	285, 973, 1452, 1669
DraIII	CACNNNGTG	2 cut(s)	890, 2290
EaeI	YGGCCR	2 cut(s)	467, 849
Eam1104I	CTCTTC	2 cut(s)	270, 2187
EarI	CTCTTC	2 cut(s)	270, 2187
Eco130I	CCWWGG	4 cut(s)	302, 664, 1022, 1081
Eco32I	GATATC	1 cut(s)	1576
Eco47I	GGWCC	3 cut(s)	503, 989, 1538
Eco57I	CTGAAG	1 cut(s)	1017
EcoO109I	RGGNCCY	1 cut(s)	1538
EcoRI	GAATTC	2 cut(s)	982, 1656
EcoRII	CCWGG	6 cut(s)	327, 764, 1555, 1585, 1891, 2104
EcoRV	GATATC	1 cut(s)	1576
EcoT14I	CCWWGG	4 cut(s)	302, 664, 1022, 1081
EcoT22I	ATGCAT	1 cut(s)	1195
ErhI	CCWWGG	4 cut(s)	302, 664, 1022, 1081
FaqI	GGGAC	5 cut(s)	384, 1119, 1292, 1551, 2033
FauI	CCCGC	3 cut(s)	630, 970, 1455
FokI	GGATG	4 cut(s)	105, 285, 499, 1547
FspBI	CTAG	6 cut(s)	53, 266, 1044, 1068, 2022, 2079
FspI	TGCGCA	1 cut(s)	1942
GlaI	GCGC	8 cut(s)	463, 650, 718, 1042, 1324, 1858, 1942, 2121
GsuI	CTGGAG	2 cut(s)	1608, 2088
HaeII	RGCGCY	1 cut(s)	1044
HaeIII	GGCC	9 cut(s)	7, 469, 621, 663, 793, 851, 1265, 1627, 1719
HapII	CCGG	4 cut(s)	708, 992, 1359, 1707
HhaI	GCGC	8 cut(s)	464, 651, 719, 1043, 1325, 1859, 1943, 2122
Hin6I	GCGC	8 cut(s)	462, 649, 717, 1041, 1323, 1857, 1941, 2120
HinP1I	GCGC	8 cut(s)	462, 649, 717, 1041, 1323, 1857, 1941, 2120
HincII	GTYRAC	2 cut(s)	684, 1870
HindII	GTYRAC	2 cut(s)	684, 1870
HindIII	AAGCTT	2 cut(s)	1111, 2200
HinfI	GANTC	9 cut(s)	215, 434, 473, 508, 528, 641, 1336, 1747, 2296
HpaI	GTTAAC	1 cut(s)	1870
HpaII	CCGG	4 cut(s)	708, 992, 1359, 1707
HphI	GGTGA	8 cut(s)	440, 747, 821, 1069, 1313, 1405, 2086, 2201
Hpy166II	GTNNAC	7 cut(s)	100, 684, 1294, 1697, 1870, 1903, 2282
Hpy188I	TCNGA	5 cut(s)	136, 288, 543, 1981, 2275
Hpy188III	TCNNGA	7 cut(s)	322, 409, 1202, 1774, 2061, 2135, 2179
Hpy8I	GTNNAC	7 cut(s)	100, 684, 1294, 1697, 1870, 1903, 2282
Hpy99I	CGWCG	1 cut(s)	1421
HpyCH4III	ACNGT	7 cut(s)	920, 1006, 1036, 1123, 1880, 2047, 2086
HpyCH4IV	ACGT	1 cut(s)	2250
HpyF3I	CTNAG	4 cut(s)	285, 973, 1452, 1669
HpySE526I	ACGT	1 cut(s)	2250
HspAI	GCGC	8 cut(s)	462, 649, 717, 1041, 1323, 1857, 1941, 2120
KflI	GGGWCCC	1 cut(s)	1538
KspAI	GTTAAC	1 cut(s)	1870
KspI	CCGCGG	2 cut(s)	1463, 1785
LmnI	GCTCC	4 cut(s)	188, 581, 674, 2003
LweI	GCATC	6 cut(s)	127, 307, 811, 1165, 1202, 1569
MaeI	CTAG	6 cut(s)	53, 266, 1044, 1068, 2022, 2079
MaeII	ACGT	1 cut(s)	2250
MaeIII	GTNAC	6 cut(s)	154, 703, 1075, 1786, 1850, 2074
MbiI	CCGCTC	1 cut(s)	625
MboII	GAAGA	9 cut(s)	281, 284, 287, 345, 424, 443, 901, 1262, 2204
MfeI	CAATTG	3 cut(s)	927, 1944, 2301
MflI	RGATCY	5 cut(s)	547, 895, 1257, 1888, 2063
MhlI	GDGCHC	1 cut(s)	2000
MlsI	TGGCCA	2 cut(s)	469, 851
MluNI	TGGCCA	2 cut(s)	469, 851
MlyI	GAGTC	2 cut(s)	1756, 2305
Mox20I	TGGCCA	2 cut(s)	469, 851
Mph1103I	ATGCAT	1 cut(s)	1195
MscI	TGGCCA	2 cut(s)	469, 851
MseI	TTAA	9 cut(s)	948, 1101, 1443, 1566, 1725, 1761, 1869, 1917, 2205
MslI	CAYNNNNRTG	4 cut(s)	246, 517, 962, 1685
Msp20I	TGGCCA	2 cut(s)	469, 851
MspA1I	CMGCKG	4 cut(s)	689, 977, 1462, 1784
MspCI	CTTAAG	1 cut(s)	1565
MspI	CCGG	4 cut(s)	708, 992, 1359, 1707
MspR9I	CCNGG	7 cut(s)	329, 766, 1360, 1557, 1587, 1893, 2106
MunI	CAATTG	3 cut(s)	927, 1944, 2301
MvaI	CCWGG	6 cut(s)	329, 766, 1557, 1587, 1893, 2106
MvnI	CGCG	6 cut(s)	649, 651, 719, 1462, 1784, 2122
NciI	CCSGG	1 cut(s)	1360
NcoI	CCATGG	2 cut(s)	664, 1022
NlaIV	GGNNCC	7 cut(s)	184, 670, 1539, 1540, 1809, 1890, 2065
NmuCI	GTSAC	3 cut(s)	154, 1075, 2074
NsbI	TGCGCA	1 cut(s)	1942
NsiI	ATGCAT	1 cut(s)	1195
NspI	RCATGY	2 cut(s)	1388, 1868
PagI	TCATGA	1 cut(s)	1773
PciI	ACATGT	2 cut(s)	1384, 1864
PcsI	WCGNNNNNNNCGW	1 cut(s)	861
PfeI	GAWTC	7 cut(s)	215, 434, 473, 508, 528, 641, 1336
PfoI	TCCNGGA	2 cut(s)	1891, 2104
PinAI	ACCGGT	1 cut(s)	707
Ple19I	CGATCG	1 cut(s)	356
PleI	GAGTC	2 cut(s)	1755, 2304
PpsI	GAGTC	2 cut(s)	1755, 2304
Ppu21I	YACGTR	1 cut(s)	2251
PpuMI	RGGWCCY	1 cut(s)	1538
PscI	ACATGT	2 cut(s)	1384, 1864
PshBI	ATTAAT	1 cut(s)	1443
Psp5II	RGGWCCY	1 cut(s)	1538
Psp6I	CCWGG	6 cut(s)	327, 764, 1555, 1585, 1891, 2104
PspGI	CCWGG	6 cut(s)	327, 764, 1555, 1585, 1891, 2104
PspN4I	GGNNCC	7 cut(s)	184, 670, 1539, 1540, 1809, 1890, 2065
PspPI	GGNCC	5 cut(s)	503, 620, 989, 1538, 1625
PspPPI	RGGWCCY	1 cut(s)	1538
PstI	CTGCAG	4 cut(s)	613, 1174, 1756, 2220
PstNI	CAGNNNCTG	2 cut(s)	617, 2153
PsuI	RGATCY	5 cut(s)	547, 895, 1257, 1888, 2063
PvuI	CGATCG	1 cut(s)	356
PvuII	CAGCTG	1 cut(s)	689
RsaI	GTAC	5 cut(s)	57, 101, 1003, 1048, 2211
RsaNI	GTAC	5 cut(s)	56, 100, 1002, 1047, 2210
RseI	CAYNNNNRTG	4 cut(s)	246, 517, 962, 1685
SacII	CCGCGG	2 cut(s)	1463, 1785
SaqAI	TTAA	9 cut(s)	948, 1101, 1443, 1566, 1725, 1761, 1869, 1917, 2205
Sau96I	GGNCC	5 cut(s)	503, 620, 989, 1538, 1625
SbfI	CCTGCAGG	1 cut(s)	2220
ScaI	AGTACT	2 cut(s)	57, 1048
SchI	GAGTC	2 cut(s)	1756, 2305
ScrFI	CCNGG	7 cut(s)	329, 766, 1360, 1557, 1587, 1893, 2106
SdaI	CCTGCAGG	1 cut(s)	2220
SduI	GDGCHC	1 cut(s)	2000
SfaNI	GCATC	6 cut(s)	127, 307, 811, 1165, 1202, 1569
SfcI	CTRYAG	4 cut(s)	609, 1170, 1752, 2216
Sfr303I	CCGCGG	2 cut(s)	1463, 1785
SgrBI	CCGCGG	2 cut(s)	1463, 1785
SinI	GGWCC	3 cut(s)	503, 989, 1538
SmiMI	CAYNNNNRTG	4 cut(s)	246, 517, 962, 1685
SmlI	CTYRAG	4 cut(s)	374, 1200, 1565, 2069
SmoI	CTYRAG	4 cut(s)	374, 1200, 1565, 2069
Sse8387I	CCTGCAGG	1 cut(s)	2220
SsiI	CCGC	9 cut(s)	8, 623, 647, 977, 1132, 1460, 1462, 1782, 1784
SspMI	CTAG	6 cut(s)	53, 266, 1044, 1068, 2022, 2079
StyD4I	CCNGG	7 cut(s)	327, 764, 1358, 1555, 1585, 1891, 2104
StyI	CCWWGG	4 cut(s)	302, 664, 1022, 1081
TaaI	ACNGT	7 cut(s)	920, 1006, 1036, 1123, 1880, 2047, 2086
TaiI	ACGT	1 cut(s)	2253
TaqI	TCGA	7 cut(s)	352, 437, 639, 855, 1118, 1273, 1532
TaqII	GACCGA	1 cut(s)	520
TatI	WGTACW	3 cut(s)	55, 99, 1046
TauI	GCSGC	2 cut(s)	10, 649
TfiI	GAWTC	7 cut(s)	215, 434, 473, 508, 528, 641, 1336
Tru1I	TTAA	9 cut(s)	948, 1101, 1443, 1566, 1725, 1761, 1869, 1917, 2205
Tru9I	TTAA	9 cut(s)	948, 1101, 1443, 1566, 1725, 1761, 1869, 1917, 2205
TscAI	CASTG	9 cut(s)	566, 891, 925, 1332, 1585, 1677, 1885, 2050, 2091
TseFI	GTSAC	3 cut(s)	154, 1075, 2074
Tsp45I	GTSAC	3 cut(s)	154, 1075, 2074
TspDTI	ATGAA	5 cut(s)	216, 1380, 1504, 1518, 1971
TspGWI	ACGGA	1 cut(s)	1502
TspRI	CASTG	9 cut(s)	566, 891, 925, 1332, 1585, 1677, 1885, 2050, 2091
Vha464I	CTTAAG	1 cut(s)	1565
VpaK11BI	GGWCC	3 cut(s)	503, 989, 1538
VspI	ATTAAT	1 cut(s)	1443
XapI	RAATTY	5 cut(s)	554, 697, 982, 1148, 1656
XceI	RCATGY	2 cut(s)	1388, 1868
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2135
XspI	CTAG	6 cut(s)	53, 266, 1044, 1068, 2022, 2079
ZrmI	AGTACT	2 cut(s)	57, 1048
Zsp2I	ATGCAT	1 cut(s)	1195

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.