MD09G1156000.v1.1

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007469
Physical Location & Seq
Forward (+)
12430531 .. 12438867
8337 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1156000.v1.1.491

Sequence Viewer

Length: 1449 bp
ATGGAAACTGAGAATGGTAATGTTAAGCAAGCTGAAGATGCTAAACTACTTGCCAATGAAGCATATAAAGCTCATAAATTTGCTCAAGCTATTGATCTGTATACACAAGCGATTGAACTAAACAGTCAGAATGCTGTATACTGGGCCAATCGTTCAATTGCCCACCTTAAACTGGAGGAATATGGTAGCGCCATACAAGATGCTTCCAAGGCTATTGAAGTTGATCCCAAATATTCAAAGGGATACTACAGACGAGGTGCAGCTTATCTTGCTATGGGGAAATTTAAAGAAGCTTTAAAGGATTTTCAGCAGGTCAAAAGAATCTGTCCAAATGATCCTGATGCTACCAAGAAATTGAAGGAATGTGAGAAAGCAGTGATGAAGCTTAAATTTGCTGAAGCAATATCTGCACCGGAGGCTGAAAGGCGTCCTGTAGCTGATTCTATTAATTATCGTTCTATAGATGTGGAACCACAATATGCCGGTGCAAGGATAGAAGGGGATGTTGTTACTTTGGATTTTGTGAAGAAAATGATGGAGGATTTCAAGAATCAGAAGAATTTACACCAACGATATGCGTTCCAAATTGTCTTACAGACGAAAGAAATGCTGCAAGCCATGCCTTCTCTGGTTGATATAAATATTCCTAAGGGCAATCATTTTACTGTTTGTGGTGACGTACATGGGCAGTTCTATGATCTTCTAAATATTTTTGAGCTTAACGGGCTTCCTTCAGATGAGAATCCATATCTATTCAATGGAGATTTTGTGGACAGGGGATCTTTTTCTTTGGAGGTGATCCTAACATTGTTTGCTTTTAAGTGCATGTCTCCATCAGCAATATATCTCGCAAGAGGAAATCATGAGAGCAAGAGCATGAATAGGATTTATGGTTTTGAGGGCGAGGTCCAGTCCAAATTAAGCGACAAGTTTGTGGAGCTGTTTGCTGAAGTTTTTTGTTGTCTACCGCTGGCTCATGTAATAAATGGGAAGGTTTTTGTAGTCCATGGGGGTCTTTTCAGCGTTGATGGAGTGAAACTCAGCGACATTAGAGCAATTAATCGGTTTTGTGAACCTCCTGAGGAAGGGTTAATGTGTGAATTGCTGTGGAGTGATCCACAGCCTGCACCTGGAAGAGGACCAAGCAAGCGAGGTGTAGGTCTTTCTTTTGGTGGCGATGTGACAAAAAGATTTTTGCAGGAAAACAATTTAGATTTAGTTGTGCGATCTCATGAAGTAAAGGACGAGGGTTATGAGATTGAGCATGATGGGAAACTTATCACTGTCTTTTCTGCTCCAAATTACTGTGATCAGATGGGTAACAAGGGTGCCTTTATCCGTTTTGAAGCACCAGATTTGAAGCCTAACATTGTTACTTTCTCAGCAGTGCCACATCCTGACGTCAAGCCAATGGCATATGCGAACAACTTCCTCCGAATGTTTCAGTAA

Protein Analysis

483

Amino Acids

54.22

Weight (kDa)

5.86

Isoelectric Point (pI)

35.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_BSK1_C PF25575 7 - 83 2.4e-08 Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_1 PF00515 80 - 112 3.6e-09 Tetratricopeptide repeat
TPR_2 PF07719 80 - 112 3.8e-07 Tetratricopeptide repeat
PPP5 PF08321 119 - 212 1.3e-28 PPP5 TPR repeat region
Metallophos PF00149 221 - 414 2.6e-38 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1404
Acc36I ACCTGC 1 cut(s) 301
AccB1I GGYRCC 1 cut(s) 1328
AccI GTMKAC 3 cut(s) 101, 138, 964
AciI CCGC 1 cut(s) 968
AclWI GGATC 5 cut(s) 218, 329, 787, 793, 1109
AcsI RAATTY 4 cut(s) 77, 281, 389, 559
AcuI CTGAAG 4 cut(s) 54, 417, 717, 969
AcyI GRCGYC 2 cut(s) 427, 1401
AfaI GTAC 1 cut(s) 681
AfiI CCNNNNNNNGG 5 cut(s) 172, 489, 1085, 1130, 1136
AgsI TTSAA 9 cut(s) 116, 156, 218, 237, 358, 547, 757, 1346, 1360
AjnI CCWGG 1 cut(s) 1129
AluBI AGCT 9 cut(s) 32, 71, 89, 263, 293, 385, 437, 718, 940
AluI AGCT 9 cut(s) 32, 71, 89, 263, 293, 385, 437, 718, 940
Alw26I GTCTC 1 cut(s) 834
AlwI GGATC 5 cut(s) 218, 329, 787, 793, 1109
AoxI GGCC 1 cut(s) 144
ApeKI GCWGC 2 cut(s) 260, 610
ApoI RAATTY 4 cut(s) 77, 281, 389, 559
AseI ATTAAT 2 cut(s) 447, 1059
Asp700I GAANNNNTTC 1 cut(s) 1427
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 3 cut(s) 144, 907, 1139
AsuHPI GGTGA 2 cut(s) 686, 808
AvaII GGWCC 2 cut(s) 907, 1139
AxyI CCTNAGG 2 cut(s) 648, 1080
BanI GGYRCC 1 cut(s) 1328
BbvI GCAGC 2 cut(s) 272, 597
BccI CCATC 5 cut(s) 529, 841, 1022, 1262, 1309
BcgI CGANNNNNNTGC 2 cut(s) 589, 623
BciT130I CCWGG 1 cut(s) 1131
BciVI GTATCC 1 cut(s) 236
BclI TGATCA 1 cut(s) 1309
BcoDI GTCTC 1 cut(s) 834
BfmI CTRYAG 3 cut(s) 247, 432, 459
BfoI RGCGCY 1 cut(s) 192
BfuAI ACCTGC 1 cut(s) 301
BfuI GTATCC 1 cut(s) 236
BisI GCNGC 2 cut(s) 261, 611
BlsI GCNGC 2 cut(s) 262, 612
Bme1390I CCNGG 1 cut(s) 1131
Bme18I GGWCC 2 cut(s) 907, 1139
BmgT120I GGNCC 3 cut(s) 144, 907, 1139
BmiI GGNNCC 2 cut(s) 471, 1330
BmrFI CCNGG 1 cut(s) 1131
BmrI ACTGGG 1 cut(s) 151
BmsI GCATC 3 cut(s) 28, 190, 331
BmuI ACTGGG 1 cut(s) 151
BplI GAGNNNNNCTC 2 cut(s) 1023, 1055
BpmI CTGGAG 1 cut(s) 194
BpuEI CTTGAG 1 cut(s) 69
BsaBI GATNNNNATC 1 cut(s) 741
BsaHI GRCGYC 2 cut(s) 427, 1401
BsaJI CCNNGG 2 cut(s) 207, 1006
BsaWI WCCGGW 1 cut(s) 412
BsaXI ACNNNNNCTCC 2 cut(s) 753, 783
Bsc4I CCNNNNNNNGG 5 cut(s) 172, 489, 1085, 1130, 1136
Bse118I RCCGGY 1 cut(s) 482
Bse1I ACTGG 3 cut(s) 146, 177, 910
Bse21I CCTNAGG 2 cut(s) 648, 1080
Bse8I GATNNNNATC 1 cut(s) 741
BseBI CCWGG 1 cut(s) 1131
BseDI CCNNGG 2 cut(s) 207, 1006
BseGI GGATG 2 cut(s) 508, 1393
BseJI GATNNNNATC 1 cut(s) 741
BseLI CCNNNNNNNGG 5 cut(s) 172, 489, 1085, 1130, 1136
BseMII CTCAG 3 cut(s) 1054, 1071, 1395
BseNI ACTGG 3 cut(s) 146, 177, 910
BseXI GCAGC 2 cut(s) 272, 597
BsgI GTGCAG 3 cut(s) 279, 393, 1110
BshFI GGCC 1 cut(s) 146
BshNI GGYRCC 1 cut(s) 1328
BsiSI CCGG 2 cut(s) 413, 483
BslI CCNNNNNNNGG 5 cut(s) 172, 489, 1085, 1130, 1136
BsmAI GTCTC 1 cut(s) 834
BsmI GAATGC 1 cut(s) 136
BsnI GGCC 1 cut(s) 146
Bsp143I GATC 9 cut(s) 94, 223, 334, 697, 779, 798, 1114, 1226, 1309
Bsp19I CCATGG 1 cut(s) 1006
BspACI CCGC 1 cut(s) 968
BspANI GGCC 1 cut(s) 146
BspCNI CTCAG 3 cut(s) 1053, 1072, 1394
BspHI TCATGA 2 cut(s) 862, 1231
BspLI GGNNCC 2 cut(s) 471, 1330
BspMI ACCTGC 1 cut(s) 301
BspPI GGATC 5 cut(s) 218, 329, 787, 793, 1109
BspT107I GGYRCC 1 cut(s) 1328
BsrFI RCCGGY 1 cut(s) 482
BsrI ACTGG 3 cut(s) 146, 177, 910
BssAI RCCGGY 1 cut(s) 482
BssECI CCNNGG 2 cut(s) 207, 1006
BssMI GATC 9 cut(s) 94, 223, 334, 697, 779, 798, 1114, 1226, 1309
BssNAI GTATAC 2 cut(s) 102, 139
BssNI GRCGYC 2 cut(s) 427, 1401
BssT1I CCWWGG 2 cut(s) 207, 1006
Bst1107I GTATAC 2 cut(s) 102, 139
Bst2UI CCWGG 1 cut(s) 1131
Bst4CI ACNGT 4 cut(s) 125, 667, 1285, 1307
Bst6I CTCTTC 1 cut(s) 1129
BstACI GRCGYC 2 cut(s) 427, 1401
BstAPI GCANNNNNTGC 2 cut(s) 407, 619
BstC8I GCNNGC 5 cut(s) 30, 615, 972, 1125, 1148
BstDEI CTNAG 5 cut(s) 9, 648, 1040, 1080, 1381
BstDSI CCRYGG 1 cut(s) 1006
BstENI CCTNNNNNAGG 2 cut(s) 1083, 1134
BstF5I GGATG 2 cut(s) 508, 1393
BstH2I RGCGCY 1 cut(s) 192
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 9 cut(s) 97, 226, 337, 700, 782, 801, 1117, 1229, 1312
BstMAI GTCTC 1 cut(s) 834
BstMBI GATC 9 cut(s) 94, 223, 334, 697, 779, 798, 1114, 1226, 1309
BstMWI GCNNNNNNNGC 9 cut(s) 38, 59, 68, 209, 269, 407, 416, 619, 724
BstNI CCWGG 1 cut(s) 1131
BstNSI RCATGY 1 cut(s) 829
BstSCI CCNGG 1 cut(s) 1129
BstSFI CTRYAG 3 cut(s) 247, 432, 459
BstV1I GCAGC 2 cut(s) 272, 597
BstX2I RGATCY 1 cut(s) 779
BstYI RGATCY 1 cut(s) 779
BstZ17I GTATAC 2 cut(s) 102, 139
Bsu36I CCTNAGG 2 cut(s) 648, 1080
BsuI GTATCC 1 cut(s) 236
BsuRI GGCC 1 cut(s) 146
BtgI CCRYGG 1 cut(s) 1006
BtgZI GCGATG 1 cut(s) 1191
BtsCI GGATG 2 cut(s) 508, 1393
BtsI GCAGTG 2 cut(s) 381, 1392
BtsIMutI CAGTG 3 cut(s) 381, 1281, 1392
BveI ACCTGC 1 cut(s) 301
Cac8I GCNNGC 5 cut(s) 30, 615, 972, 1125, 1148
CciI TCATGA 2 cut(s) 862, 1231
CfoI GCGC 1 cut(s) 191
Cfr10I RCCGGY 1 cut(s) 482
Cfr13I GGNCC 3 cut(s) 144, 907, 1139
CseI GACGC 1 cut(s) 416
Csp6I GTAC 1 cut(s) 680
CviAII CATG 9 cut(s) 619, 683, 826, 863, 877, 977, 1007, 1232, 1265
CviQI GTAC 1 cut(s) 680
DdeI CTNAG 5 cut(s) 9, 648, 1040, 1080, 1381
DpnI GATC 9 cut(s) 96, 225, 336, 699, 781, 800, 1116, 1228, 1311
DpnII GATC 9 cut(s) 94, 223, 334, 697, 779, 798, 1114, 1226, 1309
DraI TTTAAA 2 cut(s) 286, 297
Eam1104I CTCTTC 1 cut(s) 1129
EarI CTCTTC 1 cut(s) 1129
Eco130I CCWWGG 2 cut(s) 207, 1006
Eco47I GGWCC 2 cut(s) 907, 1139
Eco57I CTGAAG 4 cut(s) 54, 417, 717, 969
Eco81I CCTNAGG 2 cut(s) 648, 1080
EcoNI CCTNNNNNAGG 2 cut(s) 1083, 1134
EcoRII CCWGG 1 cut(s) 1129
EcoT14I CCWWGG 2 cut(s) 207, 1006
ErhI CCWWGG 2 cut(s) 207, 1006
FaeI CATG 9 cut(s) 622, 686, 829, 866, 880, 980, 1010, 1235, 1268
FalI AAGNNNNNCTT 2 cut(s) 1316, 1348
FatI CATG 9 cut(s) 618, 682, 825, 862, 876, 976, 1006, 1231, 1264
FauNDI CATATG 1 cut(s) 1417
FbaI TGATCA 1 cut(s) 1309
FblI GTMKAC 3 cut(s) 101, 138, 964
Fnu4HI GCNGC 2 cut(s) 261, 611
FokI GGATG 2 cut(s) 515, 1380
Fsp4HI GCNGC 2 cut(s) 261, 611
GlaI GCGC 1 cut(s) 190
GluI GCNGC 2 cut(s) 261, 611
GsuI CTGGAG 1 cut(s) 194
HaeII RGCGCY 1 cut(s) 192
HaeIII GGCC 1 cut(s) 146
HapII CCGG 2 cut(s) 413, 483
HgaI GACGC 1 cut(s) 416
HhaI GCGC 1 cut(s) 191
Hin1I GRCGYC 2 cut(s) 427, 1401
Hin1II CATG 9 cut(s) 622, 686, 829, 866, 880, 980, 1010, 1235, 1268
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HindIII AAGCTT 2 cut(s) 291, 383
HinfI GANTC 4 cut(s) 321, 440, 550, 742
HpaII CCGG 2 cut(s) 413, 483
HphI GGTGA 2 cut(s) 686, 808
Hpy166II GTNNAC 5 cut(s) 102, 139, 772, 965, 1073
Hpy188I TCNGA 5 cut(s) 129, 555, 736, 1314, 1436
Hpy188III TCNNGA 6 cut(s) 338, 547, 863, 1079, 1232, 1397
Hpy8I GTNNAC 5 cut(s) 102, 139, 772, 965, 1073
HpyAV CCTTC 6 cut(s) 352, 491, 633, 741, 985, 1079
HpyCH4III ACNGT 4 cut(s) 125, 667, 1285, 1307
HpyCH4IV ACGT 2 cut(s) 678, 1401
HpyCH4V TGCA 7 cut(s) 260, 410, 488, 613, 825, 1127, 1198
HpyF10VI GCNNNNNNNGC 9 cut(s) 38, 59, 68, 209, 269, 407, 416, 619, 724
HpyF3I CTNAG 5 cut(s) 9, 648, 1040, 1080, 1381
HpySE526I ACGT 2 cut(s) 678, 1401
Hsp92I GRCGYC 2 cut(s) 427, 1401
Hsp92II CATG 9 cut(s) 622, 686, 829, 866, 880, 980, 1010, 1235, 1268
HspAI GCGC 1 cut(s) 189
Ksp22I TGATCA 1 cut(s) 1309
Kzo9I GATC 9 cut(s) 94, 223, 334, 697, 779, 798, 1114, 1226, 1309
LmnI GCTCC 2 cut(s) 937, 1300
Lsp1109I GCAGC 2 cut(s) 272, 597
LweI GCATC 3 cut(s) 28, 190, 331
MaeII ACGT 2 cut(s) 678, 1401
MaeIII GTNAC 5 cut(s) 508, 674, 1180, 1319, 1372
MalI GATC 9 cut(s) 96, 225, 336, 699, 781, 800, 1116, 1228, 1311
MboI GATC 9 cut(s) 94, 223, 334, 697, 779, 798, 1114, 1226, 1309
MboII GAAGA 5 cut(s) 47, 538, 568, 692, 1146
MfeI CAATTG 1 cut(s) 156
MflI RGATCY 1 cut(s) 779
MroXI GAANNNNTTC 1 cut(s) 1427
MspA1I CMGCKG 1 cut(s) 970
MspI CCGG 2 cut(s) 413, 483
MspR9I CCNGG 1 cut(s) 1131
MunI CAATTG 1 cut(s) 156
Mva1269I GAATGC 1 cut(s) 136
MvaI CCWGG 1 cut(s) 1131
MwoI GCNNNNNNNGC 9 cut(s) 38, 59, 68, 209, 269, 407, 416, 619, 724
NcoI CCATGG 1 cut(s) 1006
NdeI CATATG 1 cut(s) 1417
NdeII GATC 9 cut(s) 94, 223, 334, 697, 779, 798, 1114, 1226, 1309
NlaIII CATG 9 cut(s) 622, 686, 829, 866, 880, 980, 1010, 1235, 1268
NlaIV GGNNCC 2 cut(s) 471, 1330
NmuCI GTSAC 2 cut(s) 674, 1180
NspI RCATGY 1 cut(s) 829
PagI TCATGA 2 cut(s) 862, 1231
PctI GAATGC 1 cut(s) 136
PdmI GAANNNNTTC 1 cut(s) 1427
PfeI GAWTC 4 cut(s) 321, 440, 550, 742
PkrI GCNGC 2 cut(s) 262, 612
PshBI ATTAAT 2 cut(s) 447, 1059
Psp6I CCWGG 1 cut(s) 1129
PspGI CCWGG 1 cut(s) 1129
PspN4I GGNNCC 2 cut(s) 471, 1330
PspPI GGNCC 3 cut(s) 144, 907, 1139
PsuI RGATCY 1 cut(s) 779
RsaI GTAC 1 cut(s) 681
RsaNI GTAC 1 cut(s) 680
SatI GCNGC 2 cut(s) 261, 611
Sau3AI GATC 9 cut(s) 94, 223, 334, 697, 779, 798, 1114, 1226, 1309
Sau96I GGNCC 3 cut(s) 144, 907, 1139
ScrFI CCNGG 1 cut(s) 1131
SfaNI GCATC 3 cut(s) 28, 190, 331
SfcI CTRYAG 3 cut(s) 247, 432, 459
SinI GGWCC 2 cut(s) 907, 1139
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
SsiI CCGC 1 cut(s) 968
SspI AATATT 3 cut(s) 233, 643, 709
StyD4I CCNGG 1 cut(s) 1129
StyI CCWWGG 2 cut(s) 207, 1006
TaaI ACNGT 4 cut(s) 125, 667, 1285, 1307
TaiI ACGT 2 cut(s) 681, 1404
TfiI GAWTC 4 cut(s) 321, 440, 550, 742
TscAI CASTG 3 cut(s) 381, 1288, 1392
TseFI GTSAC 2 cut(s) 674, 1180
TseI GCWGC 2 cut(s) 260, 610
Tsp45I GTSAC 2 cut(s) 674, 1180
TspDTI ATGAA 4 cut(s) 72, 395, 893, 1248
TspGWI ACGGA 1 cut(s) 1328
TspRI CASTG 3 cut(s) 381, 1288, 1392
VpaK11BI GGWCC 2 cut(s) 907, 1139
VspI ATTAAT 2 cut(s) 447, 1059
XagI CCTNNNNNAGG 2 cut(s) 1083, 1134
XapI RAATTY 4 cut(s) 77, 281, 389, 559
XceI RCATGY 1 cut(s) 829
XcmI CCANNNNNNNNNTGG 1 cut(s) 625
XmiI GTMKAC 3 cut(s) 101, 138, 964
XmnI GAANNNNTTC 1 cut(s) 1427
ZraI GACGTC 1 cut(s) 1402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.