Rh2AG483200

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0007469
Physical Location & Seq
Reverse (-)
70384905 .. 70392202
7298 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG483200.1

Sequence Viewer

Length: 1692 bp
ATGTGCCGCCTTACTTCTTGTAGAGGACAATTCATGCCCATTATGGACAATTCTAATGTTTTGCGAGCTGAAGAAATTAAGCTTCTTGCAAATGAATCATTTAAAGCCCATAAATATTCTCAGGCTATTGATCTGTACACCCAAGCGATTGAACTAAACAGCAAGAATGCCGTATACTATGCGAATCGTTCGTTTGCTCACCTTAAACTGGAGGAATATGGAAGTGCCATACAAGATGCTTCAATGGCTATTGAAGTTGATCCTAGATATTCAAAGGGATACTACAGGCGAGGTGCAGCTTCTCTTGCTATGGGGAAATTCAAAGAGGCACTAAAAGATTTCCAGCAGGTTAAAAAAATCTGTCCAAATGATCCTGATGCTACCAAGAAACTGAAGGAATGTGAGAAGGCAGTGATGAAGCTTAAATTTGCTGAGGCAATTTCTGTACCAGAGTCTCAAAGGCGTCCCATAGCTGATTCTATTAATTATCGTTCTATAGGAACGGGTCCCAGTTCATCATCAGTGCCCACTGAAGTTACCGTAGCAGTTCTGGCAATAGCAGCTGGGGTAGCGATGGTTATGATGGGAACAGCAGTGGCTGCAGTTGTGGCCACAATAGTGGTGGTAGTCTTGGTGGTTCTGGGGGCACGTTGGTGGCGTGGCTTCCATGGTGGGGTTTATACCACAAGTCGAGTAAATGACCTAGAAGTGGAGCCACAATATTCTGGTGCAAGGATAGAAGGAGATGTTGTTACTTTGGATTTTGTGAAGAAAATGATGGAGGATTTTAAGAATCAGAAGACTTTACACCAAAGATATGCATTCCAAATTGTGTTACAAACGAAAGAAATGTTGCAAACACTGCCTTCTCTTGTTGATATAAATGTTCCAAATGGCAACCATTTTACTGTTTGTGGGGACGTACATGGTCAGTTCTATGATCTCCTAAATATTTTTGAGCTTAATGGGCTTCCTTCAGAGGAGAATCCGTATCTATTCAATGGTGATTTTGTGGACAGAGGATCATTTTCGCTGGAGGTCATTCTAACACTGTTTGCATTTAAGTGCATGTCTCCATCAGCAATATATCTTGCAAGGGGTAACCATGAAAGCAAAAGCATGAACAGGATATATGGTTTTGAGGGCGAGGTCCAGTCCAAGTTAAGCGAAAAATTTGTGGAACTATTTGCTGAAGTGTTTTGTTGTTTACCGTTGGCTCATGTAATAAACGGCAAGGTGTTTGTAGTTCATGGGGGTCTTTTTAGTGTTGATGGGGTGAAACTGAGTGATATTAAAGCAATTAATCGGTTTTGTGAACCTCCTGAGGAAGGATTGATGTGTGAATTGCTATGGAGTGATCCACAACCTGCACCTGGAAGAGGCCCTAGCAAGCGAGGTGTAGGTCTTTCTTTTGGTGGTGATGTGACAAAAAGATTTTTGCAGGACAACAATTTAGATTTAGTTGTGCGCTCTCATGAAGTAAAGGATGAGGGATATGAGATTGAGCATGATGGTAAACTCATCACTGTATTTTCTGCCCCAAATTACTGTGATCAGATGGGTAACAAAGGCGCCTTTATCCGTTTCGAAGCACCAGATTTGAAGCCAAATATAGTCACTTTTTCAGCAGTGCCGCATCCTGATGTGAAGCCAATGGCATATGCAAATAACTTCCTCCGACTCTTTCAGTAG

Protein Analysis

563

Amino Acids

62.61

Weight (kDa)

6.35

Isoelectric Point (pI)

36.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_BSK1_C PF25575 27 - 95 3e-07 Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_1 PF00515 92 - 124 2.8e-07 Tetratricopeptide repeat
TPR_2 PF07719 92 - 124 7.3e-06 Tetratricopeptide repeat
PPP5 PF08321 228 - 293 2.6e-19 PPP5 TPR repeat region
Metallophos PF00149 302 - 495 2e-38 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 337, 1375
AccB1I GGYRCC 1 cut(s) 1571
AccI GTMKAC 1 cut(s) 174
AciI CCGC 2 cut(s) 7, 1634
AclWI GGATC 4 cut(s) 254, 365, 1030, 1352
AcoI YGGCCR 1 cut(s) 609
AcsI RAATTY 3 cut(s) 317, 425, 1172
AcuI CTGAAG 5 cut(s) 90, 413, 552, 960, 1212
AcyI GRCGYC 2 cut(s) 463, 1572
AfaI GTAC 3 cut(s) 137, 447, 924
AfiI CCNNNNNNNGG 6 cut(s) 208, 673, 709, 1328, 1373, 1379
AgsI TTSAA 7 cut(s) 152, 243, 254, 273, 322, 1000, 1603
AjnI CCWGG 1 cut(s) 1372
AleI CACNNNNGTG 2 cut(s) 617, 652
AluBI AGCT 7 cut(s) 68, 82, 299, 421, 473, 563, 961
AluI AGCT 7 cut(s) 68, 82, 299, 421, 473, 563, 961
Alw26I GTCTC 2 cut(s) 459, 1077
AlwI GGATC 4 cut(s) 254, 365, 1030, 1352
AlwNI CAGNNNCTG 1 cut(s) 599
AoxI GGCC 2 cut(s) 609, 1381
ApeKI GCWGC 3 cut(s) 296, 560, 599
ApoI RAATTY 3 cut(s) 317, 425, 1172
AseI ATTAAT 2 cut(s) 483, 1302
AspLEI GCGC 2 cut(s) 1470, 1574
AspS9I GGNCC 3 cut(s) 506, 1150, 1382
AsuHPI GGTGA 4 cut(s) 191, 1016, 1288, 1430
AsuII TTCGAA 1 cut(s) 1587
AvaII GGWCC 2 cut(s) 506, 1150
AxyI CCTNAGG 1 cut(s) 1323
BaeGI GKGCMC 2 cut(s) 528, 649
BalI TGGCCA 1 cut(s) 611
BanI GGYRCC 1 cut(s) 1571
BbsI GAAGAC 1 cut(s) 806
BbvCI CCTCAGC 1 cut(s) 432
BbvI GCAGC 3 cut(s) 308, 572, 586
BccI CCATC 7 cut(s) 568, 577, 772, 1084, 1265, 1505, 1552
BceAI ACGGC 2 cut(s) 155, 1246
BciT130I CCWGG 1 cut(s) 1374
BciVI GTATCC 1 cut(s) 272
BclI TGATCA 1 cut(s) 1552
BcoDI GTCTC 2 cut(s) 459, 1077
BfaI CTAG 3 cut(s) 264, 704, 1386
BfmI CTRYAG 3 cut(s) 283, 495, 600
BfoI RGCGCY 1 cut(s) 1575
BfuAI ACCTGC 2 cut(s) 337, 1375
BfuI GTATCC 1 cut(s) 272
BisI GCNGC 5 cut(s) 7, 297, 561, 600, 1634
BlsI GCNGC 5 cut(s) 8, 298, 562, 601, 1635
Bme1390I CCNGG 1 cut(s) 1374
Bme18I GGWCC 2 cut(s) 506, 1150
BmgT120I GGNCC 3 cut(s) 506, 1150, 1382
BmiI GGNNCC 4 cut(s) 507, 508, 714, 1573
BmrFI CCNGG 1 cut(s) 1374
BmrI ACTGGG 1 cut(s) 504
BmsI GCATC 3 cut(s) 226, 367, 1645
BmuI ACTGGG 1 cut(s) 504
BpiI GAAGAC 1 cut(s) 806
BpmI CTGGAG 2 cut(s) 230, 1055
Bpu10I CCTNAGC 1 cut(s) 432
Bpu14I TTCGAA 1 cut(s) 1587
BsaHI GRCGYC 2 cut(s) 463, 1572
BsaJI CCNNGG 1 cut(s) 667
BsaXI ACNNNNNCTCC 4 cut(s) 735, 765, 974, 1004
Bsc4I CCNNNNNNNGG 6 cut(s) 208, 673, 709, 1328, 1373, 1379
Bse1I ACTGG 3 cut(s) 213, 510, 1153
Bse21I CCTNAGG 1 cut(s) 1323
BseBI CCWGG 1 cut(s) 1374
BseDI CCNNGG 1 cut(s) 667
BseGI GGATG 2 cut(s) 1492, 1636
BseLI CCNNNNNNNGG 6 cut(s) 208, 673, 709, 1328, 1373, 1379
BseMII CTCAG 4 cut(s) 134, 423, 1274, 1314
BseNI ACTGG 3 cut(s) 213, 510, 1153
BseRI GAGGAG 1 cut(s) 995
BseSI GKGCMC 2 cut(s) 528, 649
BseXI GCAGC 3 cut(s) 308, 572, 586
BseYI CCCAGC 1 cut(s) 563
BsgI GTGCAG 2 cut(s) 315, 1353
BshFI GGCC 2 cut(s) 611, 1383
BshNI GGYRCC 1 cut(s) 1571
BslFI GGGAC 3 cut(s) 450, 492, 932
BslI CCNNNNNNNGG 6 cut(s) 208, 673, 709, 1328, 1373, 1379
BsmAI GTCTC 2 cut(s) 459, 1077
BsmFI GGGAC 3 cut(s) 450, 492, 932
BsmI GAATGC 2 cut(s) 172, 821
BsnI GGCC 2 cut(s) 611, 1383
Bsp119I TTCGAA 1 cut(s) 1587
Bsp1286I GDGCHC 2 cut(s) 528, 649
Bsp1407I TGTACA 1 cut(s) 135
Bsp143I GATC 7 cut(s) 130, 259, 370, 940, 1022, 1357, 1552
Bsp19I CCATGG 1 cut(s) 667
BspACI CCGC 2 cut(s) 7, 1634
BspANI GGCC 2 cut(s) 611, 1383
BspCNI CTCAG 4 cut(s) 133, 424, 1275, 1315
BspHI TCATGA 1 cut(s) 1474
BspLI GGNNCC 4 cut(s) 507, 508, 714, 1573
BspMAI CTGCAG 1 cut(s) 604
BspMI ACCTGC 2 cut(s) 337, 1375
BspPI GGATC 4 cut(s) 254, 365, 1030, 1352
BspT104I TTCGAA 1 cut(s) 1587
BspT107I GGYRCC 1 cut(s) 1571
BsrGI TGTACA 1 cut(s) 135
BsrI ACTGG 3 cut(s) 213, 510, 1153
BssECI CCNNGG 1 cut(s) 667
BssMI GATC 7 cut(s) 130, 259, 370, 940, 1022, 1357, 1552
BssNAI GTATAC 1 cut(s) 175
BssNI GRCGYC 2 cut(s) 463, 1572
BssT1I CCWWGG 1 cut(s) 667
Bst1107I GTATAC 1 cut(s) 175
Bst2UI CCWGG 1 cut(s) 1374
Bst4CI ACNGT 6 cut(s) 541, 910, 1053, 1212, 1528, 1550
Bst6I CTCTTC 1 cut(s) 1372
BstACI GRCGYC 2 cut(s) 463, 1572
BstAPI GCANNNNNTGC 2 cut(s) 599, 862
BstAUI TGTACA 1 cut(s) 135
BstBI TTCGAA 1 cut(s) 1587
BstC8I GCNNGC 2 cut(s) 66, 1391
BstDEI CTNAG 4 cut(s) 120, 432, 1283, 1323
BstDSI CCRYGG 1 cut(s) 667
BstEII GGTNACC 1 cut(s) 1100
BstENI CCTNNNNNAGG 2 cut(s) 1326, 1377
BstF5I GGATG 2 cut(s) 1492, 1636
BstH2I RGCGCY 1 cut(s) 1575
BstHHI GCGC 2 cut(s) 1470, 1574
BstKTI GATC 7 cut(s) 133, 262, 373, 943, 1025, 1360, 1555
BstMAI GTCTC 2 cut(s) 459, 1077
BstMBI GATC 7 cut(s) 130, 259, 370, 940, 1022, 1357, 1552
BstMWI GCNNNNNNNGC 9 cut(s) 245, 305, 551, 560, 569, 599, 608, 862, 967
BstNI CCWGG 1 cut(s) 1374
BstNSI RCATGY 1 cut(s) 1072
BstPI GGTNACC 1 cut(s) 1100
BstSCI CCNGG 1 cut(s) 1372
BstSFI CTRYAG 3 cut(s) 283, 495, 600
BstSLI GKGCMC 2 cut(s) 528, 649
BstV1I GCAGC 3 cut(s) 308, 572, 586
BstV2I GAAGAC 1 cut(s) 806
BstXI CCANNNNNNTGG 1 cut(s) 619
BstZ17I GTATAC 1 cut(s) 175
Bsu36I CCTNAGG 1 cut(s) 1323
BsuI GTATCC 1 cut(s) 272
BsuRI GGCC 2 cut(s) 611, 1383
BtgI CCRYGG 1 cut(s) 667
BtgZI GCGATG 1 cut(s) 587
BtsCI GGATG 2 cut(s) 1492, 1636
BtsI GCAGTG 4 cut(s) 417, 600, 860, 1635
BtsIMutI CAGTG 8 cut(s) 417, 528, 528, 600, 860, 1049, 1524, 1635
BveI ACCTGC 2 cut(s) 337, 1375
Cac8I GCNNGC 2 cut(s) 66, 1391
CaiI CAGNNNCTG 1 cut(s) 599
CciI TCATGA 1 cut(s) 1474
CfoI GCGC 2 cut(s) 1470, 1574
Cfr13I GGNCC 3 cut(s) 506, 1150, 1382
CseI GACGC 1 cut(s) 452
Csp6I GTAC 3 cut(s) 136, 446, 923
CspCI CAANNNNNGTGG 2 cut(s) 603, 638
CviQI GTAC 3 cut(s) 136, 446, 923
DdeI CTNAG 4 cut(s) 120, 432, 1283, 1323
DinI GGCGCC 1 cut(s) 1573
DpnI GATC 7 cut(s) 132, 261, 372, 942, 1024, 1359, 1554
DpnII GATC 7 cut(s) 130, 259, 370, 940, 1022, 1357, 1552
DraI TTTAAA 1 cut(s) 103
EaeI YGGCCR 1 cut(s) 609
Eam1104I CTCTTC 1 cut(s) 1372
EarI CTCTTC 1 cut(s) 1372
Eco130I CCWWGG 1 cut(s) 667
Eco47I GGWCC 2 cut(s) 506, 1150
Eco57I CTGAAG 5 cut(s) 90, 413, 552, 960, 1212
Eco81I CCTNAGG 1 cut(s) 1323
Eco91I GGTNACC 1 cut(s) 1100
EcoNI CCTNNNNNAGG 2 cut(s) 1326, 1377
EcoO109I RGGNCCY 2 cut(s) 506, 1382
EcoO65I GGTNACC 1 cut(s) 1100
EcoRII CCWGG 1 cut(s) 1372
EcoT14I CCWWGG 1 cut(s) 667
EcoT22I ATGCAT 1 cut(s) 823
EgeI GGCGCC 1 cut(s) 1573
EheI GGCGCC 1 cut(s) 1573
ErhI CCWWGG 1 cut(s) 667
FaqI GGGAC 3 cut(s) 450, 492, 932
FauNDI CATATG 1 cut(s) 1660
FbaI TGATCA 1 cut(s) 1552
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 5 cut(s) 7, 297, 561, 600, 1634
FokI GGATG 2 cut(s) 1499, 1623
Fsp4HI GCNGC 5 cut(s) 7, 297, 561, 600, 1634
FspBI CTAG 3 cut(s) 264, 704, 1386
GlaI GCGC 2 cut(s) 1469, 1573
GluI GCNGC 5 cut(s) 7, 297, 561, 600, 1634
GsaI CCCAGC 1 cut(s) 567
GsuI CTGGAG 2 cut(s) 230, 1055
HaeII RGCGCY 1 cut(s) 1575
HaeIII GGCC 2 cut(s) 611, 1383
HgaI GACGC 1 cut(s) 452
HhaI GCGC 2 cut(s) 1470, 1574
Hin1I GRCGYC 2 cut(s) 463, 1572
Hin6I GCGC 2 cut(s) 1468, 1572
HinP1I GCGC 2 cut(s) 1468, 1572
HindIII AAGCTT 2 cut(s) 80, 419
HinfI GANTC 7 cut(s) 95, 184, 452, 476, 793, 985, 1680
HphI GGTGA 4 cut(s) 191, 1016, 1288, 1430
Hpy166II GTNNAC 6 cut(s) 138, 175, 1015, 1208, 1316, 1517
Hpy188I TCNGA 4 cut(s) 798, 979, 1557, 1679
Hpy188III TCNNGA 4 cut(s) 374, 1322, 1475, 1640
Hpy8I GTNNAC 6 cut(s) 138, 175, 1015, 1208, 1316, 1517
HpyAV CCTTC 6 cut(s) 388, 400, 734, 876, 984, 1322
HpyCH4III ACNGT 6 cut(s) 541, 910, 1053, 1212, 1528, 1550
HpyCH4IV ACGT 2 cut(s) 649, 921
HpyF10VI GCNNNNNNNGC 9 cut(s) 245, 305, 551, 560, 569, 599, 608, 862, 967
HpyF3I CTNAG 4 cut(s) 120, 432, 1283, 1323
HpySE526I ACGT 2 cut(s) 649, 921
Hsp92I GRCGYC 2 cut(s) 463, 1572
HspAI GCGC 2 cut(s) 1468, 1572
KasI GGCGCC 1 cut(s) 1571
KflI GGGWCCC 1 cut(s) 506
Ksp22I TGATCA 1 cut(s) 1552
Kzo9I GATC 7 cut(s) 130, 259, 370, 940, 1022, 1357, 1552
LmnI GCTCC 1 cut(s) 712
Lsp1109I GCAGC 3 cut(s) 308, 572, 586
LweI GCATC 3 cut(s) 226, 367, 1645
MaeI CTAG 3 cut(s) 264, 704, 1386
MaeII ACGT 2 cut(s) 649, 921
MaeIII GTNAC 7 cut(s) 535, 751, 834, 1100, 1423, 1562, 1615
MalI GATC 7 cut(s) 132, 261, 372, 942, 1024, 1359, 1554
MboI GATC 7 cut(s) 130, 259, 370, 940, 1022, 1357, 1552
MboII GAAGA 4 cut(s) 83, 781, 811, 1389
MhlI GDGCHC 2 cut(s) 528, 649
MlsI TGGCCA 1 cut(s) 611
MluNI TGGCCA 1 cut(s) 611
Mly113I GGCGCC 1 cut(s) 1572
MlyI GAGTC 2 cut(s) 461, 1674
Mox20I TGGCCA 1 cut(s) 611
Mph1103I ATGCAT 1 cut(s) 823
MscI TGGCCA 1 cut(s) 611
MslI CAYNNNNRTG 4 cut(s) 617, 652, 1063, 1641
Msp20I TGGCCA 1 cut(s) 611
MspA1I CMGCKG 1 cut(s) 563
MspR9I CCNGG 1 cut(s) 1374
Mva1269I GAATGC 2 cut(s) 172, 821
MvaI CCWGG 1 cut(s) 1374
MwoI GCNNNNNNNGC 9 cut(s) 245, 305, 551, 560, 569, 599, 608, 862, 967
NarI GGCGCC 1 cut(s) 1572
NcoI CCATGG 1 cut(s) 667
NdeI CATATG 1 cut(s) 1660
NdeII GATC 7 cut(s) 130, 259, 370, 940, 1022, 1357, 1552
NlaIV GGNNCC 4 cut(s) 507, 508, 714, 1573
NmuCI GTSAC 2 cut(s) 1423, 1615
NsiI ATGCAT 1 cut(s) 823
NspI RCATGY 1 cut(s) 1072
NspV TTCGAA 1 cut(s) 1587
OliI CACNNNNGTG 2 cut(s) 617, 652
PagI TCATGA 1 cut(s) 1474
PcsI WCGNNNNNNNCGW 1 cut(s) 655
PctI GAATGC 2 cut(s) 172, 821
PfeI GAWTC 5 cut(s) 95, 184, 476, 793, 985
PkrI GCNGC 5 cut(s) 8, 298, 562, 601, 1635
PleI GAGTC 2 cut(s) 460, 1674
PluTI GGCGCC 1 cut(s) 1575
PpsI GAGTC 2 cut(s) 460, 1674
PpuMI RGGWCCY 1 cut(s) 506
PshBI ATTAAT 2 cut(s) 483, 1302
Psp5II RGGWCCY 1 cut(s) 506
Psp6I CCWGG 1 cut(s) 1372
PspEI GGTNACC 1 cut(s) 1100
PspFI CCCAGC 1 cut(s) 563
PspGI CCWGG 1 cut(s) 1372
PspN4I GGNNCC 4 cut(s) 507, 508, 714, 1573
PspPI GGNCC 3 cut(s) 506, 1150, 1382
PspPPI RGGWCCY 1 cut(s) 506
PstI CTGCAG 1 cut(s) 604
PstNI CAGNNNCTG 1 cut(s) 599
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 3 cut(s) 137, 447, 924
RsaNI GTAC 3 cut(s) 136, 446, 923
RseI CAYNNNNRTG 4 cut(s) 617, 652, 1063, 1641
SatI GCNGC 5 cut(s) 7, 297, 561, 600, 1634
Sau3AI GATC 7 cut(s) 130, 259, 370, 940, 1022, 1357, 1552
Sau96I GGNCC 3 cut(s) 506, 1150, 1382
SchI GAGTC 2 cut(s) 461, 1674
ScrFI CCNGG 1 cut(s) 1374
SduI GDGCHC 2 cut(s) 528, 649
SfaNI GCATC 3 cut(s) 226, 367, 1645
SfcI CTRYAG 3 cut(s) 283, 495, 600
SfoI GGCGCC 1 cut(s) 1573
SfuI TTCGAA 1 cut(s) 1587
SinI GGWCC 2 cut(s) 506, 1150
SmiMI CAYNNNNRTG 4 cut(s) 617, 652, 1063, 1641
SsiI CCGC 2 cut(s) 7, 1634
SspDI GGCGCC 1 cut(s) 1571
SspI AATATT 3 cut(s) 116, 722, 952
SspMI CTAG 3 cut(s) 264, 704, 1386
StyD4I CCNGG 1 cut(s) 1372
StyI CCWWGG 1 cut(s) 667
TaaI ACNGT 6 cut(s) 541, 910, 1053, 1212, 1528, 1550
TaiI ACGT 2 cut(s) 652, 924
TaqI TCGA 2 cut(s) 691, 1587
TatI WGTACW 1 cut(s) 135
TauI GCSGC 2 cut(s) 9, 1636
TfiI GAWTC 5 cut(s) 95, 184, 476, 793, 985
TscAI CASTG 8 cut(s) 417, 528, 535, 600, 867, 1056, 1531, 1635
TseFI GTSAC 2 cut(s) 1423, 1615
TseI GCWGC 3 cut(s) 296, 560, 599
Tsp45I GTSAC 2 cut(s) 1423, 1615
TspDTI ATGAA 8 cut(s) 22, 108, 431, 504, 1122, 1136, 1238, 1491
TspGWI ACGGA 2 cut(s) 978, 1571
TspRI CASTG 8 cut(s) 417, 528, 535, 600, 867, 1056, 1531, 1635
VpaK11BI GGWCC 2 cut(s) 506, 1150
VspI ATTAAT 2 cut(s) 483, 1302
XagI CCTNNNNNAGG 2 cut(s) 1326, 1377
XapI RAATTY 3 cut(s) 317, 425, 1172
XceI RCATGY 1 cut(s) 1072
XcmI CCANNNNNNNNNTGG 1 cut(s) 619
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 3 cut(s) 264, 704, 1386
Zsp2I ATGCAT 1 cut(s) 823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.