RchiOBHm_Chr2g0150851

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007469
Physical Location & Seq
Reverse (-)
68515446 .. 68522698
7253 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52010

Sequence Viewer

Length: 1458 bp
ATGCCCATTATGGAAAATGACAATTCTAATGTTTCGCGAGCTGAAGAAATTAAGCTTCTTGCAAATGAAGCATTTAAAGCCCATAAATATTCTCAGGCTATTGATCTGTACACCCAAGCGATTGAACTAAACAGCAAGAATGCCGTATACTATGCGAATCGTTCGTTTGCTCACCTTAAACTGGAGGAATATGGAAGTGCCATACAAGATGCTTCAATGGCTATTGAAGTTGATCCTAGATATTCAAAGGGATACTACAGGCGAGGTGCAGCTTCTCTTGCTATGGGGAAATTCAAAGAGGCACTAAAAGATTTCCAGCAGGTTAAAAAAATCTGTCCAAATGATCCTGATGCTACCAAGAAACTGAAGGAATGTGAGAAGGCAGTGATGAAGCTTAAATTTGCTGAGGCAATTTCTGTACCAGAGTCTCAAAGGCGTCCCATAGCTGATTCTATTAATTATCGTTCTATAGAAGTGGAGCCACAATATTCTGGTGCAAGGATAGAAGGAGATGTTGTTACTTTGGATTTTGTGAAGAAAATGATGGAGGATTTTAAGAATCAGAAGACTTTACACCAAAGATATGCATTCCAAATTGTGTTACAAACGAAAGAAATGTTGCAAACGCTGCCTTCTCTTGTTGATATAAATGTTCCAAATGGCAACCATTTTACTGTTTGTGGGGACGTACATGGTCAGTTCTATGATCTCCTAAACATTTTTGAGCTTAATGGGCTTCCTTCAGAGGAGAATCCGTATCTATTCAATGGTGATTTTGTGGACAGAGGATCATTTTCGCTGGAGGTCATTCTAACACTGTTTGCATTTAAGTGCATGTCTCCATCAGCAATATATCTTGCAAGAGGGAACCATGAAAGCAAAAGCATGAACAGGATATATGGTTTTGAGGGCGAGGTCCAGTCCAAGTTAAGCGAAAAATTTGTGGAACTATTTGCTGAAGTGTTTTGTTGTTTACCGTTGGCTCATGTAATAAACGGCAAGGTGTTTGTAGTTCATGGGGGTCTTTTTAGTGTTGATGGGGTGAAACTGAGTGATATTAAAGCGATTAATCGGTTTTGTGAACCTCCTGAGGAAGGATTGATGTGTGAATTGCTATGGAGTGATCCACAACCTGCACCTGGAAGAGGCCCTAGCAAGCGAGGTGTAGGTCTTTCTTTTGGTGGTGATGTGACAAAAAGATTTTTGCAGGACAACAATTTAGATTTAGTTGTGCGCTCTCATGAAGTAAAGGATGAGGGATATGAGATTGAGCATGATGGTAAACTCATCACTGTATTTTCTGCCCCAAATTACTGTGATCAGATGGGTAACAAAGGCGCCTTTATCCGTTTTGAAGCACCAGATTTGAAGCCAAATATAGTCACTTTTTCAGCAGTGCCGCATCCTGATGTGAAGCCAATGGCATATGCGAATAACTTCCTCCGACTCTTTCAGTAG

Protein Analysis

485

Amino Acids

54.62

Weight (kDa)

5.86

Isoelectric Point (pI)

38.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_BSK1_C PF25575 13 - 86 2e-08 Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_1 PF00515 83 - 115 2.4e-07 Tetratricopeptide repeat
TPR_2 PF07719 83 - 115 6.1e-06 Tetratricopeptide repeat
PPP5 PF08321 122 - 215 5.8e-29 PPP5 TPR repeat region
Metallophos PF00149 224 - 417 1.5e-38 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 310, 1141
AccB1I GGYRCC 1 cut(s) 1337
AccI GTMKAC 1 cut(s) 147
AccII CGCG 1 cut(s) 37
AciI CCGC 1 cut(s) 1400
AclWI GGATC 4 cut(s) 227, 338, 796, 1118
AcsI RAATTY 3 cut(s) 290, 398, 938
AcuI CTGAAG 4 cut(s) 63, 386, 726, 978
AcyI GRCGYC 2 cut(s) 436, 1338
AfaI GTAC 3 cut(s) 110, 420, 690
AfiI CCNNNNNNNGG 4 cut(s) 181, 1094, 1139, 1145
AgsI TTSAA 8 cut(s) 125, 216, 227, 246, 295, 766, 1355, 1369
AjnI CCWGG 1 cut(s) 1138
AluBI AGCT 6 cut(s) 41, 55, 272, 394, 446, 727
AluI AGCT 6 cut(s) 41, 55, 272, 394, 446, 727
Alw26I GTCTC 2 cut(s) 432, 843
AlwI GGATC 4 cut(s) 227, 338, 796, 1118
AoxI GGCC 1 cut(s) 1147
ApeKI GCWGC 2 cut(s) 269, 628
ApoI RAATTY 3 cut(s) 290, 398, 938
AseI ATTAAT 2 cut(s) 456, 1068
Asp700I GAANNNNTTC 1 cut(s) 1436
AspLEI GCGC 2 cut(s) 1236, 1340
AspS9I GGNCC 2 cut(s) 916, 1148
AsuHPI GGTGA 4 cut(s) 164, 782, 1054, 1196
AvaII GGWCC 1 cut(s) 916
AxyI CCTNAGG 1 cut(s) 1089
BanI GGYRCC 1 cut(s) 1337
BbsI GAAGAC 1 cut(s) 572
BbvCI CCTCAGC 1 cut(s) 405
BbvI GCAGC 2 cut(s) 281, 615
BccI CCATC 5 cut(s) 538, 850, 1031, 1271, 1318
BceAI ACGGC 2 cut(s) 128, 1012
BciT130I CCWGG 1 cut(s) 1140
BciVI GTATCC 1 cut(s) 245
BclI TGATCA 1 cut(s) 1318
BcoDI GTCTC 2 cut(s) 432, 843
BfaI CTAG 2 cut(s) 237, 1152
BfmI CTRYAG 2 cut(s) 256, 468
BfoI RGCGCY 1 cut(s) 1341
BfuAI ACCTGC 2 cut(s) 310, 1141
BfuI GTATCC 1 cut(s) 245
BisI GCNGC 3 cut(s) 270, 629, 1400
BlsI GCNGC 3 cut(s) 271, 630, 1401
Bme1390I CCNGG 1 cut(s) 1140
Bme18I GGWCC 1 cut(s) 916
BmgT120I GGNCC 2 cut(s) 916, 1148
BmiI GGNNCC 3 cut(s) 480, 869, 1339
BmrFI CCNGG 1 cut(s) 1140
BmsI GCATC 3 cut(s) 199, 340, 1411
BpiI GAAGAC 1 cut(s) 572
BpmI CTGGAG 2 cut(s) 203, 821
Bpu10I CCTNAGC 1 cut(s) 405
BsaHI GRCGYC 2 cut(s) 436, 1338
BsaXI ACNNNNNCTCC 4 cut(s) 501, 531, 740, 770
Bsc4I CCNNNNNNNGG 4 cut(s) 181, 1094, 1139, 1145
Bse1I ACTGG 2 cut(s) 186, 919
Bse21I CCTNAGG 1 cut(s) 1089
BseBI CCWGG 1 cut(s) 1140
BseGI GGATG 2 cut(s) 1258, 1402
BseLI CCNNNNNNNGG 4 cut(s) 181, 1094, 1139, 1145
BseMII CTCAG 4 cut(s) 107, 396, 1040, 1080
BseNI ACTGG 2 cut(s) 186, 919
BseRI GAGGAG 1 cut(s) 761
BseXI GCAGC 2 cut(s) 281, 615
BsgI GTGCAG 2 cut(s) 288, 1119
Bsh1236I CGCG 1 cut(s) 37
BshFI GGCC 1 cut(s) 1149
BshNI GGYRCC 1 cut(s) 1337
BslFI GGGAC 2 cut(s) 423, 698
BslI CCNNNNNNNGG 4 cut(s) 181, 1094, 1139, 1145
BsmAI GTCTC 2 cut(s) 432, 843
BsmFI GGGAC 2 cut(s) 423, 698
BsmI GAATGC 2 cut(s) 145, 587
BsnI GGCC 1 cut(s) 1149
Bsp1407I TGTACA 1 cut(s) 108
Bsp143I GATC 7 cut(s) 103, 232, 343, 706, 788, 1123, 1318
Bsp68I TCGCGA 1 cut(s) 37
BspACI CCGC 1 cut(s) 1400
BspANI GGCC 1 cut(s) 1149
BspCNI CTCAG 4 cut(s) 106, 397, 1041, 1081
BspFNI CGCG 1 cut(s) 37
BspHI TCATGA 1 cut(s) 1240
BspLI GGNNCC 3 cut(s) 480, 869, 1339
BspMI ACCTGC 2 cut(s) 310, 1141
BspPI GGATC 4 cut(s) 227, 338, 796, 1118
BspT107I GGYRCC 1 cut(s) 1337
BsrGI TGTACA 1 cut(s) 108
BsrI ACTGG 2 cut(s) 186, 919
BssMI GATC 7 cut(s) 103, 232, 343, 706, 788, 1123, 1318
BssNAI GTATAC 1 cut(s) 148
BssNI GRCGYC 2 cut(s) 436, 1338
Bst1107I GTATAC 1 cut(s) 148
Bst2UI CCWGG 1 cut(s) 1140
Bst4CI ACNGT 5 cut(s) 676, 819, 978, 1294, 1316
Bst6I CTCTTC 1 cut(s) 1138
BstACI GRCGYC 2 cut(s) 436, 1338
BstAPI GCANNNNNTGC 1 cut(s) 628
BstAUI TGTACA 1 cut(s) 108
BstC8I GCNNGC 2 cut(s) 39, 1157
BstDEI CTNAG 4 cut(s) 93, 405, 1049, 1089
BstENI CCTNNNNNAGG 2 cut(s) 1092, 1143
BstF5I GGATG 2 cut(s) 1258, 1402
BstFNI CGCG 1 cut(s) 37
BstH2I RGCGCY 1 cut(s) 1341
BstHHI GCGC 2 cut(s) 1236, 1340
BstKTI GATC 7 cut(s) 106, 235, 346, 709, 791, 1126, 1321
BstMAI GTCTC 2 cut(s) 432, 843
BstMBI GATC 7 cut(s) 103, 232, 343, 706, 788, 1123, 1318
BstMWI GCNNNNNNNGC 6 cut(s) 68, 77, 218, 278, 628, 733
BstNI CCWGG 1 cut(s) 1140
BstNSI RCATGY 1 cut(s) 838
BstSCI CCNGG 1 cut(s) 1138
BstSFI CTRYAG 2 cut(s) 256, 468
BstUI CGCG 1 cut(s) 37
BstV1I GCAGC 2 cut(s) 281, 615
BstV2I GAAGAC 1 cut(s) 572
BstZ17I GTATAC 1 cut(s) 148
Bsu36I CCTNAGG 1 cut(s) 1089
BsuI GTATCC 1 cut(s) 245
BsuRI GGCC 1 cut(s) 1149
BtsCI GGATG 2 cut(s) 1258, 1402
BtsI GCAGTG 2 cut(s) 390, 1401
BtsIMutI CAGTG 4 cut(s) 390, 815, 1290, 1401
BtuMI TCGCGA 1 cut(s) 37
BveI ACCTGC 2 cut(s) 310, 1141
Cac8I GCNNGC 2 cut(s) 39, 1157
CciI TCATGA 1 cut(s) 1240
CfoI GCGC 2 cut(s) 1236, 1340
Cfr13I GGNCC 2 cut(s) 916, 1148
CseI GACGC 1 cut(s) 425
Csp6I GTAC 3 cut(s) 109, 419, 689
CviAII CATG 8 cut(s) 692, 835, 872, 886, 986, 1016, 1241, 1274
CviQI GTAC 3 cut(s) 109, 419, 689
DdeI CTNAG 4 cut(s) 93, 405, 1049, 1089
DinI GGCGCC 1 cut(s) 1339
DpnI GATC 7 cut(s) 105, 234, 345, 708, 790, 1125, 1320
DpnII GATC 7 cut(s) 103, 232, 343, 706, 788, 1123, 1318
DraI TTTAAA 1 cut(s) 76
Eam1104I CTCTTC 1 cut(s) 1138
EarI CTCTTC 1 cut(s) 1138
Eco47I GGWCC 1 cut(s) 916
Eco57I CTGAAG 4 cut(s) 63, 386, 726, 978
Eco81I CCTNAGG 1 cut(s) 1089
EcoNI CCTNNNNNAGG 2 cut(s) 1092, 1143
EcoO109I RGGNCCY 1 cut(s) 1148
EcoRII CCWGG 1 cut(s) 1138
EcoT22I ATGCAT 1 cut(s) 589
EgeI GGCGCC 1 cut(s) 1339
EheI GGCGCC 1 cut(s) 1339
FaeI CATG 8 cut(s) 695, 838, 875, 889, 989, 1019, 1244, 1277
FaqI GGGAC 2 cut(s) 423, 698
FatI CATG 8 cut(s) 691, 834, 871, 885, 985, 1015, 1240, 1273
FauNDI CATATG 1 cut(s) 1426
FbaI TGATCA 1 cut(s) 1318
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 3 cut(s) 270, 629, 1400
FokI GGATG 2 cut(s) 1265, 1389
Fsp4HI GCNGC 3 cut(s) 270, 629, 1400
FspBI CTAG 2 cut(s) 237, 1152
GlaI GCGC 2 cut(s) 1235, 1339
GluI GCNGC 3 cut(s) 270, 629, 1400
GsuI CTGGAG 2 cut(s) 203, 821
HaeII RGCGCY 1 cut(s) 1341
HaeIII GGCC 1 cut(s) 1149
HgaI GACGC 1 cut(s) 425
HhaI GCGC 2 cut(s) 1236, 1340
Hin1I GRCGYC 2 cut(s) 436, 1338
Hin1II CATG 8 cut(s) 695, 838, 875, 889, 989, 1019, 1244, 1277
Hin6I GCGC 2 cut(s) 1234, 1338
HinP1I GCGC 2 cut(s) 1234, 1338
HindIII AAGCTT 2 cut(s) 53, 392
HinfI GANTC 6 cut(s) 157, 425, 449, 559, 751, 1446
HphI GGTGA 4 cut(s) 164, 782, 1054, 1196
Hpy166II GTNNAC 6 cut(s) 111, 148, 781, 974, 1082, 1283
Hpy188I TCNGA 4 cut(s) 564, 745, 1323, 1445
Hpy188III TCNNGA 5 cut(s) 36, 347, 1088, 1241, 1406
Hpy8I GTNNAC 6 cut(s) 111, 148, 781, 974, 1082, 1283
HpyAV CCTTC 6 cut(s) 361, 373, 500, 642, 750, 1088
HpyCH4III ACNGT 5 cut(s) 676, 819, 978, 1294, 1316
HpyCH4IV ACGT 1 cut(s) 687
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 77, 218, 278, 628, 733
HpyF3I CTNAG 4 cut(s) 93, 405, 1049, 1089
HpySE526I ACGT 1 cut(s) 687
Hsp92I GRCGYC 2 cut(s) 436, 1338
Hsp92II CATG 8 cut(s) 695, 838, 875, 889, 989, 1019, 1244, 1277
HspAI GCGC 2 cut(s) 1234, 1338
KasI GGCGCC 1 cut(s) 1337
Ksp22I TGATCA 1 cut(s) 1318
Kzo9I GATC 7 cut(s) 103, 232, 343, 706, 788, 1123, 1318
LmnI GCTCC 1 cut(s) 478
Lsp1109I GCAGC 2 cut(s) 281, 615
LweI GCATC 3 cut(s) 199, 340, 1411
MaeI CTAG 2 cut(s) 237, 1152
MaeII ACGT 1 cut(s) 687
MaeIII GTNAC 5 cut(s) 517, 600, 1189, 1328, 1381
MalI GATC 7 cut(s) 105, 234, 345, 708, 790, 1125, 1320
MboI GATC 7 cut(s) 103, 232, 343, 706, 788, 1123, 1318
MboII GAAGA 4 cut(s) 56, 547, 577, 1155
Mly113I GGCGCC 1 cut(s) 1338
MlyI GAGTC 2 cut(s) 434, 1440
Mph1103I ATGCAT 1 cut(s) 589
MroXI GAANNNNTTC 1 cut(s) 1436
MslI CAYNNNNRTG 2 cut(s) 829, 1407
MspR9I CCNGG 1 cut(s) 1140
Mva1269I GAATGC 2 cut(s) 145, 587
MvaI CCWGG 1 cut(s) 1140
MvnI CGCG 1 cut(s) 37
MwoI GCNNNNNNNGC 6 cut(s) 68, 77, 218, 278, 628, 733
NarI GGCGCC 1 cut(s) 1338
NdeI CATATG 1 cut(s) 1426
NdeII GATC 7 cut(s) 103, 232, 343, 706, 788, 1123, 1318
NlaIII CATG 8 cut(s) 695, 838, 875, 889, 989, 1019, 1244, 1277
NlaIV GGNNCC 3 cut(s) 480, 869, 1339
NmuCI GTSAC 2 cut(s) 1189, 1381
NruI TCGCGA 1 cut(s) 37
NsiI ATGCAT 1 cut(s) 589
NspI RCATGY 1 cut(s) 838
PagI TCATGA 1 cut(s) 1240
PctI GAATGC 2 cut(s) 145, 587
PdmI GAANNNNTTC 1 cut(s) 1436
PfeI GAWTC 4 cut(s) 157, 449, 559, 751
PkrI GCNGC 3 cut(s) 271, 630, 1401
PleI GAGTC 2 cut(s) 433, 1440
PluTI GGCGCC 1 cut(s) 1341
PpsI GAGTC 2 cut(s) 433, 1440
PshBI ATTAAT 2 cut(s) 456, 1068
Psp6I CCWGG 1 cut(s) 1138
PspGI CCWGG 1 cut(s) 1138
PspN4I GGNNCC 3 cut(s) 480, 869, 1339
PspPI GGNCC 2 cut(s) 916, 1148
RruI TCGCGA 1 cut(s) 37
RsaI GTAC 3 cut(s) 110, 420, 690
RsaNI GTAC 3 cut(s) 109, 419, 689
RseI CAYNNNNRTG 2 cut(s) 829, 1407
SatI GCNGC 3 cut(s) 270, 629, 1400
Sau3AI GATC 7 cut(s) 103, 232, 343, 706, 788, 1123, 1318
Sau96I GGNCC 2 cut(s) 916, 1148
SchI GAGTC 2 cut(s) 434, 1440
ScrFI CCNGG 1 cut(s) 1140
SfaNI GCATC 3 cut(s) 199, 340, 1411
SfcI CTRYAG 2 cut(s) 256, 468
SfoI GGCGCC 1 cut(s) 1339
SinI GGWCC 1 cut(s) 916
SmiMI CAYNNNNRTG 2 cut(s) 829, 1407
SsiI CCGC 1 cut(s) 1400
SspDI GGCGCC 1 cut(s) 1337
SspI AATATT 2 cut(s) 89, 488
SspMI CTAG 2 cut(s) 237, 1152
StyD4I CCNGG 1 cut(s) 1138
TaaI ACNGT 5 cut(s) 676, 819, 978, 1294, 1316
TaiI ACGT 1 cut(s) 690
TatI WGTACW 1 cut(s) 108
TauI GCSGC 1 cut(s) 1402
TfiI GAWTC 4 cut(s) 157, 449, 559, 751
TscAI CASTG 4 cut(s) 390, 822, 1297, 1401
TseFI GTSAC 2 cut(s) 1189, 1381
TseI GCWGC 2 cut(s) 269, 628
Tsp45I GTSAC 2 cut(s) 1189, 1381
TspDTI ATGAA 6 cut(s) 81, 404, 888, 902, 1004, 1257
TspGWI ACGGA 2 cut(s) 744, 1337
TspRI CASTG 4 cut(s) 390, 822, 1297, 1401
VpaK11BI GGWCC 1 cut(s) 916
VspI ATTAAT 2 cut(s) 456, 1068
XagI CCTNNNNNAGG 2 cut(s) 1092, 1143
XapI RAATTY 3 cut(s) 290, 398, 938
XceI RCATGY 1 cut(s) 838
XmiI GTMKAC 1 cut(s) 147
XmnI GAANNNNTTC 1 cut(s) 1436
XspI CTAG 2 cut(s) 237, 1152
Zsp2I ATGCAT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.