RLG00000020512

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007469
Physical Location & Seq
Reverse (-)
65811810 .. 65817792
5983 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020512

Sequence Viewer

Length: 1698 bp
ATGTGCCGCCTTGCTTCTTGTAGAGGACAATTCATGCCCATTATGGAAAATGACAAGTCTAATGTTTCGCGAGCTGAAGAAATTAAGCTTCTTGCAAATGAAGCATTTAAAGCCCATAAATATTCTCAGGCTATTGATCTGTACACCCAAGCGATTGAACTAAACAGCAAGAATGCCGTATACTATGCGAATCGTTCGTTTGCTCACCTTAAACTGGAGGAATATGGAAGTGCCATACAAGATGCTTCAATGGCTATTGAAGTTGATCCCAGATATTCAAAGGGATACTACAGGCGAGGTGCAGCTTCTCTTGCTATGGGGAAATTCAAAGAGGCACTAAAAGATTTCCAGCAGGTTAAAAAAATCTGTCCAAATGATCCTGATGCTACCAAGAAACTGAAGGAATGTGAGAAGGCAGTGATGAAGCTTAAATTTGCTGAGGCAATTTCTGTACCAGAGTCTCAAAGGCGTCCCATAGCTGATTCTATTAATTATCGTTCTATAGGAACGGGTCCCAGTTCATCATCAGTGCCCACCGAAGTTACCGTAGCAGTTCTGGCAATAGCAGCTGGGGTAGCGATGGTTATGATGGGAACAGCAGTGGCTGCAGTTGTGGCCACAATAGTGGTGGTAGTCATGGTGGTCCTGGGGGCACGTTGGTGGCGTGGCTTCCATGGTGGGGTTTATACCACAAGTCGAGTAAATGACCTAGAAGTGGAGCCACAATATTCTGGTGCAAGGATAGAAGGAGATGTTGTTACTTTGGATTTTGTGAAGAAAATGATGGAGGATTTTAAGAATCAGAAGACCTTACACCAAAGATATGCATTCCAAATTGTGTTGCAAACGAAAGAAATGTTGCAAACGCTGCCTTCTCTTGTTGATATAAATGTTCCAAATGGCAACCATTTTACTGTTTGTGGGGACGTACATGGTCAGTTCTATGATCTCCTAAATATTTTTGAGCTTAATGGGCTTCCTTCAGAGGAGAATCCCTATCTATTCAATGGTGATTTTGTGGACAGAGGATCGTTTTCGCTGGAGGTCATTCTAACACTGTTTGCATTTAAGTGCATGTCTCCATCAGCAATATATCTTGCAAGGGGGAACCATGAAAGCAAAAGCATGAACAGGATATATGGTTTTGAGGGCGAGGTCCAGTCCAAGTTAAGCGAAAAATTTGTGGAACTATTTGCTGAAGTGTTTTGTTGTTTACCATTGGCTCATGTAATAAACGGCAAGGTGTTTGTAGTTCATGGGGGTCTTTTTAGTGTTGATGGGGTGAAACTGAGTGATATTAGAGCAATTAATCGGTTTTGTGAACCTCCTGAGGAAGGATTGATGTGTGAATTGCTATGGAGTGATCCACAACCTGCACCTGGAAGAGGCCCTAGCAAGCGAGGTGTAGGTCTTTCTTTTGGTGGTGATGTGACAAAAAGATTTTTGCAGGACAACAATTTAGATTTAGTTGTGCGCTCTCATGAAGTAAAGGATGAGGGATATGAGATTGAGCATGATGGTAAACTCATCACTGTATTTTCTGCTCCAAATTACTGTGATCAGATGGGTAACAAAGGCGCCTTTATCCGTTTTGAAGCACCAGATTTGAAGCCAAATATAGTCACTTTTTCAGCAGTGCCACATCCTGATGTGAAGCCAATGGCATATGCAAATAACTTCCTCCGACTCTTTCAGTAG

Protein Analysis

566

Amino Acids

62.84

Weight (kDa)

6.35

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_BSK1_C PF25575 23 - 97 2.5e-08 Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_1 PF00515 94 - 126 2.9e-07 Tetratricopeptide repeat
TPR_2 PF07719 94 - 126 7.4e-06 Tetratricopeptide repeat
PPP5 PF08321 230 - 295 2.6e-19 PPP5 TPR repeat region
Metallophos PF00149 304 - 497 1.5e-38 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 343, 1381
AccB1I GGYRCC 1 cut(s) 1577
AccI GTMKAC 1 cut(s) 180
AccII CGCG 1 cut(s) 70
AciI CCGC 1 cut(s) 7
AclWI GGATC 4 cut(s) 260, 371, 1036, 1358
AcoI YGGCCR 1 cut(s) 615
AcsI RAATTY 3 cut(s) 323, 431, 1178
AcuI CTGAAG 4 cut(s) 96, 419, 966, 1218
AcyI GRCGYC 2 cut(s) 469, 1578
AfaI GTAC 3 cut(s) 143, 453, 930
AfiI CCNNNNNNNGG 6 cut(s) 214, 679, 715, 1334, 1379, 1385
AgsI TTSAA 8 cut(s) 158, 249, 260, 279, 328, 1006, 1595, 1609
AjnI CCWGG 2 cut(s) 645, 1378
AleI CACNNNNGTG 2 cut(s) 623, 658
AluBI AGCT 7 cut(s) 74, 88, 305, 427, 479, 569, 967
AluI AGCT 7 cut(s) 74, 88, 305, 427, 479, 569, 967
Alw26I GTCTC 2 cut(s) 465, 1083
AlwI GGATC 4 cut(s) 260, 371, 1036, 1358
AlwNI CAGNNNCTG 1 cut(s) 605
AoxI GGCC 2 cut(s) 615, 1387
ApeKI GCWGC 4 cut(s) 302, 566, 605, 868
ApoI RAATTY 3 cut(s) 323, 431, 1178
AseI ATTAAT 2 cut(s) 489, 1308
AspLEI GCGC 2 cut(s) 1476, 1580
AspS9I GGNCC 4 cut(s) 512, 643, 1156, 1388
AsuHPI GGTGA 4 cut(s) 197, 1022, 1294, 1436
AvaII GGWCC 3 cut(s) 512, 643, 1156
AxyI CCTNAGG 1 cut(s) 1329
BaeGI GKGCMC 2 cut(s) 534, 655
BalI TGGCCA 1 cut(s) 617
BanI GGYRCC 1 cut(s) 1577
BbsI GAAGAC 1 cut(s) 812
BbvCI CCTCAGC 1 cut(s) 438
BbvI GCAGC 4 cut(s) 314, 578, 592, 855
BccI CCATC 7 cut(s) 574, 583, 778, 1090, 1271, 1511, 1558
BceAI ACGGC 2 cut(s) 161, 1252
BciT130I CCWGG 2 cut(s) 647, 1380
BciVI GTATCC 1 cut(s) 278
BclI TGATCA 1 cut(s) 1558
BcoDI GTCTC 2 cut(s) 465, 1083
BfaI CTAG 2 cut(s) 710, 1392
BfmI CTRYAG 3 cut(s) 289, 501, 606
BfoI RGCGCY 1 cut(s) 1581
BfuAI ACCTGC 2 cut(s) 343, 1381
BfuI GTATCC 1 cut(s) 278
BisI GCNGC 5 cut(s) 7, 303, 567, 606, 869
BlsI GCNGC 5 cut(s) 8, 304, 568, 607, 870
Bme1390I CCNGG 2 cut(s) 647, 1380
Bme18I GGWCC 3 cut(s) 512, 643, 1156
BmgT120I GGNCC 4 cut(s) 512, 643, 1156, 1388
BmiI GGNNCC 5 cut(s) 513, 514, 720, 1109, 1579
BmrFI CCNGG 2 cut(s) 647, 1380
BmrI ACTGGG 1 cut(s) 510
BmsI GCATC 2 cut(s) 232, 373
BmuI ACTGGG 1 cut(s) 510
BpiI GAAGAC 1 cut(s) 812
BpmI CTGGAG 2 cut(s) 236, 1061
Bpu10I CCTNAGC 1 cut(s) 438
BsaHI GRCGYC 2 cut(s) 469, 1578
BsaJI CCNNGG 2 cut(s) 646, 673
BsaXI ACNNNNNCTCC 2 cut(s) 741, 771
Bsc4I CCNNNNNNNGG 6 cut(s) 214, 679, 715, 1334, 1379, 1385
Bse1I ACTGG 3 cut(s) 219, 516, 1159
Bse21I CCTNAGG 1 cut(s) 1329
BseBI CCWGG 2 cut(s) 647, 1380
BseDI CCNNGG 2 cut(s) 646, 673
BseGI GGATG 2 cut(s) 1498, 1642
BseLI CCNNNNNNNGG 6 cut(s) 214, 679, 715, 1334, 1379, 1385
BseMII CTCAG 4 cut(s) 140, 429, 1280, 1320
BseNI ACTGG 3 cut(s) 219, 516, 1159
BseRI GAGGAG 1 cut(s) 1001
BseSI GKGCMC 2 cut(s) 534, 655
BseXI GCAGC 4 cut(s) 314, 578, 592, 855
BseYI CCCAGC 1 cut(s) 569
BsgI GTGCAG 2 cut(s) 321, 1359
Bsh1236I CGCG 1 cut(s) 70
BshFI GGCC 2 cut(s) 617, 1389
BshNI GGYRCC 1 cut(s) 1577
BslFI GGGAC 3 cut(s) 456, 498, 938
BslI CCNNNNNNNGG 6 cut(s) 214, 679, 715, 1334, 1379, 1385
BsmAI GTCTC 2 cut(s) 465, 1083
BsmFI GGGAC 3 cut(s) 456, 498, 938
BsmI GAATGC 2 cut(s) 178, 827
BsnI GGCC 2 cut(s) 617, 1389
Bsp1286I GDGCHC 2 cut(s) 534, 655
Bsp1407I TGTACA 1 cut(s) 141
Bsp143I GATC 7 cut(s) 136, 265, 376, 946, 1028, 1363, 1558
Bsp19I CCATGG 1 cut(s) 673
Bsp68I TCGCGA 1 cut(s) 70
BspACI CCGC 1 cut(s) 7
BspANI GGCC 2 cut(s) 617, 1389
BspCNI CTCAG 4 cut(s) 139, 430, 1281, 1321
BspFNI CGCG 1 cut(s) 70
BspHI TCATGA 1 cut(s) 1480
BspLI GGNNCC 5 cut(s) 513, 514, 720, 1109, 1579
BspMAI CTGCAG 1 cut(s) 610
BspMI ACCTGC 2 cut(s) 343, 1381
BspPI GGATC 4 cut(s) 260, 371, 1036, 1358
BspT107I GGYRCC 1 cut(s) 1577
BsrGI TGTACA 1 cut(s) 141
BsrI ACTGG 3 cut(s) 219, 516, 1159
BssECI CCNNGG 2 cut(s) 646, 673
BssMI GATC 7 cut(s) 136, 265, 376, 946, 1028, 1363, 1558
BssNAI GTATAC 1 cut(s) 181
BssNI GRCGYC 2 cut(s) 469, 1578
BssT1I CCWWGG 1 cut(s) 673
Bst1107I GTATAC 1 cut(s) 181
Bst2UI CCWGG 2 cut(s) 647, 1380
Bst4CI ACNGT 5 cut(s) 547, 916, 1059, 1534, 1556
Bst6I CTCTTC 1 cut(s) 1378
BstACI GRCGYC 2 cut(s) 469, 1578
BstAPI GCANNNNNTGC 2 cut(s) 605, 868
BstAUI TGTACA 1 cut(s) 141
BstC8I GCNNGC 2 cut(s) 72, 1397
BstDEI CTNAG 4 cut(s) 126, 438, 1289, 1329
BstDSI CCRYGG 1 cut(s) 673
BstENI CCTNNNNNAGG 2 cut(s) 1332, 1383
BstF5I GGATG 2 cut(s) 1498, 1642
BstFNI CGCG 1 cut(s) 70
BstH2I RGCGCY 1 cut(s) 1581
BstHHI GCGC 2 cut(s) 1476, 1580
BstKTI GATC 7 cut(s) 139, 268, 379, 949, 1031, 1366, 1561
BstMAI GTCTC 2 cut(s) 465, 1083
BstMBI GATC 7 cut(s) 136, 265, 376, 946, 1028, 1363, 1558
BstNI CCWGG 2 cut(s) 647, 1380
BstNSI RCATGY 1 cut(s) 1078
BstSCI CCNGG 2 cut(s) 645, 1378
BstSFI CTRYAG 3 cut(s) 289, 501, 606
BstSLI GKGCMC 2 cut(s) 534, 655
BstUI CGCG 1 cut(s) 70
BstV1I GCAGC 4 cut(s) 314, 578, 592, 855
BstV2I GAAGAC 1 cut(s) 812
BstXI CCANNNNNNTGG 1 cut(s) 625
BstZ17I GTATAC 1 cut(s) 181
Bsu36I CCTNAGG 1 cut(s) 1329
BsuI GTATCC 1 cut(s) 278
BsuRI GGCC 2 cut(s) 617, 1389
BtgI CCRYGG 1 cut(s) 673
BtgZI GCGATG 1 cut(s) 593
BtsCI GGATG 2 cut(s) 1498, 1642
BtsI GCAGTG 3 cut(s) 423, 606, 1641
BtsIMutI CAGTG 6 cut(s) 423, 534, 606, 1055, 1530, 1641
BtuMI TCGCGA 1 cut(s) 70
BveI ACCTGC 2 cut(s) 343, 1381
Cac8I GCNNGC 2 cut(s) 72, 1397
CaiI CAGNNNCTG 1 cut(s) 605
CciI TCATGA 1 cut(s) 1480
CfoI GCGC 2 cut(s) 1476, 1580
Cfr13I GGNCC 4 cut(s) 512, 643, 1156, 1388
CseI GACGC 1 cut(s) 458
Csp6I GTAC 3 cut(s) 142, 452, 929
CspCI CAANNNNNGTGG 2 cut(s) 609, 644
CviQI GTAC 3 cut(s) 142, 452, 929
DdeI CTNAG 4 cut(s) 126, 438, 1289, 1329
DinI GGCGCC 1 cut(s) 1579
DpnI GATC 7 cut(s) 138, 267, 378, 948, 1030, 1365, 1560
DpnII GATC 7 cut(s) 136, 265, 376, 946, 1028, 1363, 1558
DraI TTTAAA 1 cut(s) 109
EaeI YGGCCR 1 cut(s) 615
Eam1104I CTCTTC 1 cut(s) 1378
EarI CTCTTC 1 cut(s) 1378
Eco130I CCWWGG 1 cut(s) 673
Eco47I GGWCC 3 cut(s) 512, 643, 1156
Eco57I CTGAAG 4 cut(s) 96, 419, 966, 1218
Eco81I CCTNAGG 1 cut(s) 1329
EcoNI CCTNNNNNAGG 2 cut(s) 1332, 1383
EcoO109I RGGNCCY 2 cut(s) 512, 1388
EcoRII CCWGG 2 cut(s) 645, 1378
EcoT14I CCWWGG 1 cut(s) 673
EcoT22I ATGCAT 1 cut(s) 829
EgeI GGCGCC 1 cut(s) 1579
EheI GGCGCC 1 cut(s) 1579
ErhI CCWWGG 1 cut(s) 673
FaqI GGGAC 3 cut(s) 456, 498, 938
FauNDI CATATG 1 cut(s) 1666
FbaI TGATCA 1 cut(s) 1558
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 5 cut(s) 7, 303, 567, 606, 869
FokI GGATG 2 cut(s) 1505, 1629
Fsp4HI GCNGC 5 cut(s) 7, 303, 567, 606, 869
FspBI CTAG 2 cut(s) 710, 1392
GlaI GCGC 2 cut(s) 1475, 1579
GluI GCNGC 5 cut(s) 7, 303, 567, 606, 869
GsaI CCCAGC 1 cut(s) 573
GsuI CTGGAG 2 cut(s) 236, 1061
HaeII RGCGCY 1 cut(s) 1581
HaeIII GGCC 2 cut(s) 617, 1389
HgaI GACGC 1 cut(s) 458
HhaI GCGC 2 cut(s) 1476, 1580
Hin1I GRCGYC 2 cut(s) 469, 1578
Hin6I GCGC 2 cut(s) 1474, 1578
HinP1I GCGC 2 cut(s) 1474, 1578
HindIII AAGCTT 2 cut(s) 86, 425
HinfI GANTC 6 cut(s) 190, 458, 482, 799, 991, 1686
HphI GGTGA 4 cut(s) 197, 1022, 1294, 1436
Hpy166II GTNNAC 6 cut(s) 144, 181, 1021, 1214, 1322, 1523
Hpy188I TCNGA 4 cut(s) 804, 985, 1563, 1685
Hpy188III TCNNGA 5 cut(s) 69, 380, 1328, 1481, 1646
Hpy8I GTNNAC 6 cut(s) 144, 181, 1021, 1214, 1322, 1523
HpyAV CCTTC 6 cut(s) 394, 406, 740, 882, 990, 1328
HpyCH4III ACNGT 5 cut(s) 547, 916, 1059, 1534, 1556
HpyCH4IV ACGT 2 cut(s) 655, 927
HpyF3I CTNAG 4 cut(s) 126, 438, 1289, 1329
HpySE526I ACGT 2 cut(s) 655, 927
Hsp92I GRCGYC 2 cut(s) 469, 1578
HspAI GCGC 2 cut(s) 1474, 1578
KasI GGCGCC 1 cut(s) 1577
KflI GGGWCCC 1 cut(s) 512
Ksp22I TGATCA 1 cut(s) 1558
Kzo9I GATC 7 cut(s) 136, 265, 376, 946, 1028, 1363, 1558
LmnI GCTCC 2 cut(s) 718, 1549
Lsp1109I GCAGC 4 cut(s) 314, 578, 592, 855
LweI GCATC 2 cut(s) 232, 373
MaeI CTAG 2 cut(s) 710, 1392
MaeII ACGT 2 cut(s) 655, 927
MaeIII GTNAC 5 cut(s) 541, 757, 1429, 1568, 1621
MalI GATC 7 cut(s) 138, 267, 378, 948, 1030, 1365, 1560
MboI GATC 7 cut(s) 136, 265, 376, 946, 1028, 1363, 1558
MboII GAAGA 4 cut(s) 89, 787, 817, 1395
MhlI GDGCHC 2 cut(s) 534, 655
MlsI TGGCCA 1 cut(s) 617
MluNI TGGCCA 1 cut(s) 617
Mly113I GGCGCC 1 cut(s) 1578
MlyI GAGTC 2 cut(s) 467, 1680
Mox20I TGGCCA 1 cut(s) 617
Mph1103I ATGCAT 1 cut(s) 829
MscI TGGCCA 1 cut(s) 617
MslI CAYNNNNRTG 4 cut(s) 623, 658, 1069, 1647
Msp20I TGGCCA 1 cut(s) 617
MspA1I CMGCKG 1 cut(s) 569
MspR9I CCNGG 2 cut(s) 647, 1380
Mva1269I GAATGC 2 cut(s) 178, 827
MvaI CCWGG 2 cut(s) 647, 1380
MvnI CGCG 1 cut(s) 70
NarI GGCGCC 1 cut(s) 1578
NcoI CCATGG 1 cut(s) 673
NdeI CATATG 1 cut(s) 1666
NdeII GATC 7 cut(s) 136, 265, 376, 946, 1028, 1363, 1558
NlaIV GGNNCC 5 cut(s) 513, 514, 720, 1109, 1579
NmuCI GTSAC 2 cut(s) 1429, 1621
NruI TCGCGA 1 cut(s) 70
NsiI ATGCAT 1 cut(s) 829
NspI RCATGY 1 cut(s) 1078
OliI CACNNNNGTG 2 cut(s) 623, 658
PagI TCATGA 1 cut(s) 1480
PcsI WCGNNNNNNNCGW 1 cut(s) 661
PctI GAATGC 2 cut(s) 178, 827
PfeI GAWTC 4 cut(s) 190, 482, 799, 991
PkrI GCNGC 5 cut(s) 8, 304, 568, 607, 870
PleI GAGTC 2 cut(s) 466, 1680
PluTI GGCGCC 1 cut(s) 1581
PpsI GAGTC 2 cut(s) 466, 1680
PpuMI RGGWCCY 1 cut(s) 512
PshBI ATTAAT 2 cut(s) 489, 1308
Psp5II RGGWCCY 1 cut(s) 512
Psp6I CCWGG 2 cut(s) 645, 1378
PspFI CCCAGC 1 cut(s) 569
PspGI CCWGG 2 cut(s) 645, 1378
PspN4I GGNNCC 5 cut(s) 513, 514, 720, 1109, 1579
PspPI GGNCC 4 cut(s) 512, 643, 1156, 1388
PspPPI RGGWCCY 1 cut(s) 512
PstI CTGCAG 1 cut(s) 610
PstNI CAGNNNCTG 1 cut(s) 605
PvuII CAGCTG 1 cut(s) 569
RruI TCGCGA 1 cut(s) 70
RsaI GTAC 3 cut(s) 143, 453, 930
RsaNI GTAC 3 cut(s) 142, 452, 929
RseI CAYNNNNRTG 4 cut(s) 623, 658, 1069, 1647
SatI GCNGC 5 cut(s) 7, 303, 567, 606, 869
Sau3AI GATC 7 cut(s) 136, 265, 376, 946, 1028, 1363, 1558
Sau96I GGNCC 4 cut(s) 512, 643, 1156, 1388
SchI GAGTC 2 cut(s) 467, 1680
ScrFI CCNGG 2 cut(s) 647, 1380
SduI GDGCHC 2 cut(s) 534, 655
SfaNI GCATC 2 cut(s) 232, 373
SfcI CTRYAG 3 cut(s) 289, 501, 606
SfoI GGCGCC 1 cut(s) 1579
SinI GGWCC 3 cut(s) 512, 643, 1156
SmiMI CAYNNNNRTG 4 cut(s) 623, 658, 1069, 1647
SsiI CCGC 1 cut(s) 7
SspDI GGCGCC 1 cut(s) 1577
SspI AATATT 3 cut(s) 122, 728, 958
SspMI CTAG 2 cut(s) 710, 1392
StyD4I CCNGG 2 cut(s) 645, 1378
StyI CCWWGG 1 cut(s) 673
TaaI ACNGT 5 cut(s) 547, 916, 1059, 1534, 1556
TaiI ACGT 2 cut(s) 658, 930
TaqI TCGA 1 cut(s) 697
TatI WGTACW 1 cut(s) 141
TauI GCSGC 1 cut(s) 9
TfiI GAWTC 4 cut(s) 190, 482, 799, 991
TscAI CASTG 6 cut(s) 423, 534, 606, 1062, 1537, 1641
TseFI GTSAC 2 cut(s) 1429, 1621
TseI GCWGC 4 cut(s) 302, 566, 605, 868
Tsp45I GTSAC 2 cut(s) 1429, 1621
TspDTI ATGAA 8 cut(s) 22, 114, 437, 510, 1128, 1142, 1244, 1497
TspGWI ACGGA 1 cut(s) 1577
TspRI CASTG 6 cut(s) 423, 534, 606, 1062, 1537, 1641
VpaK11BI GGWCC 3 cut(s) 512, 643, 1156
VspI ATTAAT 2 cut(s) 489, 1308
XagI CCTNNNNNAGG 2 cut(s) 1332, 1383
XapI RAATTY 3 cut(s) 323, 431, 1178
XceI RCATGY 1 cut(s) 1078
XcmI CCANNNNNNNNNTGG 1 cut(s) 625
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 2 cut(s) 710, 1392
Zsp2I ATGCAT 1 cut(s) 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.