MD09G1176000.v1.1

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0010585
Physical Location & Seq
Reverse (-)
15024607 .. 15024960
354 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1176000.v1.1.491

Sequence Viewer

Length: 354 bp
ATGAAGAGAGAACTTAGCTATTTGCTAATGGGGGTGAGGAGCATCAAGGTTTTGAGCCAATTGATCGGAGCTCCAAGAGTACAAAGATTAAAAAGACTAAAAAGCCATAATTCAGTGACACCAAAAGGTTATGTTCCTGTTTGTGTTGGAGTATATGGTGATACGAAGCGTTTCATGGTTCATACGACGTTGCTTCATCATGTGGAATTCTCTGAGCTGCTGCATAGATCAGCTGAAGAATATGGTTTTCCTAATGATGGAGTTTTGAGAATTCCATACGAGGCCAAGGATTTTGAAGAGTACTGGATGATCAAACGGTCCAAACCCAAAATTTACAAGGTTGAACCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

118

Amino Acids

13.66

Weight (kDa)

9.71

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 32 - 101 1.9e-18 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 3 cut(s) 206, 270, 330
AcuI CTGAAG 1 cut(s) 255
AfaI GTAC 2 cut(s) 81, 302
AfiI CCNNNNNNNGG 1 cut(s) 257
AgsI TTSAA 2 cut(s) 296, 344
AluBI AGCT 4 cut(s) 18, 71, 217, 233
AluI AGCT 4 cut(s) 18, 71, 217, 233
Alw21I GWGCWC 1 cut(s) 73
AoxI GGCC 1 cut(s) 282
ApeKI GCWGC 2 cut(s) 217, 220
ApoI RAATTY 3 cut(s) 206, 270, 330
Asp700I GAANNNNTTC 1 cut(s) 170
AspS9I GGNCC 1 cut(s) 318
AsuHPI GGTGA 2 cut(s) 46, 170
AvaII GGWCC 1 cut(s) 318
BanII GRGCYC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 2 cut(s) 204, 207
BccI CCATC 1 cut(s) 251
BclI TGATCA 1 cut(s) 309
BisI GCNGC 2 cut(s) 218, 221
BlsI GCNGC 2 cut(s) 219, 222
BmcAI AGTACT 1 cut(s) 302
Bme18I GGWCC 1 cut(s) 318
BmgT120I GGNCC 1 cut(s) 318
BmsI GCATC 1 cut(s) 51
BsaJI CCNNGG 1 cut(s) 285
Bsc4I CCNNNNNNNGG 1 cut(s) 257
Bse1I ACTGG 2 cut(s) 308, 347
BseDI CCNNGG 1 cut(s) 285
BseGI GGATG 1 cut(s) 312
BseLI CCNNNNNNNGG 1 cut(s) 257
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 2 cut(s) 308, 347
BseRI GAGGAG 1 cut(s) 52
BseXI GCAGC 2 cut(s) 204, 207
BshFI GGCC 1 cut(s) 284
BsiHKAI GWGCWC 1 cut(s) 73
BslI CCNNNNNNNGG 1 cut(s) 257
BsnI GGCC 1 cut(s) 284
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 3 cut(s) 63, 227, 309
BspANI GGCC 1 cut(s) 284
BspCNI CTCAG 1 cut(s) 205
BsrI ACTGG 2 cut(s) 308, 347
BssECI CCNNGG 1 cut(s) 285
BssMI GATC 3 cut(s) 63, 227, 309
BssT1I CCWWGG 1 cut(s) 285
Bst4CI ACNGT 1 cut(s) 318
Bst6I CTCTTC 1 cut(s) 291
BstDEI CTNAG 2 cut(s) 14, 213
BstF5I GGATG 1 cut(s) 312
BstKTI GATC 3 cut(s) 66, 230, 312
BstMBI GATC 3 cut(s) 63, 227, 309
BstV1I GCAGC 2 cut(s) 204, 207
BsuRI GGCC 1 cut(s) 284
BtsCI GGATG 1 cut(s) 312
BtsIMutI CAGTG 1 cut(s) 120
Cfr13I GGNCC 1 cut(s) 318
Csp6I GTAC 2 cut(s) 80, 301
CviAII CATG 2 cut(s) 175, 200
CviJI RGCY 7 cut(s) 18, 57, 71, 105, 217, 233, 284
CviKI_1 RGCY 7 cut(s) 18, 57, 71, 105, 217, 233, 284
CviQI GTAC 2 cut(s) 80, 301
DdeI CTNAG 2 cut(s) 14, 213
DpnI GATC 3 cut(s) 65, 229, 311
DpnII GATC 3 cut(s) 63, 227, 309
Eam1104I CTCTTC 1 cut(s) 291
EarI CTCTTC 1 cut(s) 291
Ecl136II GAGCTC 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 73
Eco47I GGWCC 1 cut(s) 318
Eco53kI GAGCTC 1 cut(s) 71
Eco57I CTGAAG 1 cut(s) 255
EcoICRI GAGCTC 1 cut(s) 71
EcoRI GAATTC 2 cut(s) 206, 270
EcoT14I CCWWGG 1 cut(s) 285
EcoT38I GRGCYC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 285
FaeI CATG 2 cut(s) 178, 203
FatI CATG 2 cut(s) 174, 199
FbaI TGATCA 1 cut(s) 309
Fnu4HI GCNGC 2 cut(s) 218, 221
FokI GGATG 1 cut(s) 319
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 2 cut(s) 218, 221
GluI GCNGC 2 cut(s) 218, 221
HaeIII GGCC 1 cut(s) 284
Hin1II CATG 2 cut(s) 178, 203
HphI GGTGA 2 cut(s) 46, 170
Hpy188I TCNGA 2 cut(s) 68, 214
Hpy99I CGWCG 1 cut(s) 190
HpyCH4III ACNGT 1 cut(s) 318
HpyCH4IV ACGT 1 cut(s) 188
HpyCH4V TGCA 1 cut(s) 223
HpyF3I CTNAG 2 cut(s) 14, 213
HpySE526I ACGT 1 cut(s) 188
Hsp92II CATG 2 cut(s) 178, 203
Ksp22I TGATCA 1 cut(s) 309
Kzo9I GATC 3 cut(s) 63, 227, 309
LmnI GCTCC 3 cut(s) 39, 68, 76
LpnPI CCDG 2 cut(s) 150, 289
Lsp1109I GCAGC 2 cut(s) 204, 207
LweI GCATC 1 cut(s) 51
MaeII ACGT 1 cut(s) 188
MaeIII GTNAC 1 cut(s) 115
MalI GATC 3 cut(s) 65, 229, 311
MboI GATC 3 cut(s) 63, 227, 309
MboII GAAGA 3 cut(s) 16, 248, 308
MfeI CAATTG 1 cut(s) 59
MhlI GDGCHC 1 cut(s) 73
MluCI AATT 5 cut(s) 59, 109, 206, 270, 330
MmeI TCCRAC 1 cut(s) 127
MnlI CCTC 2 cut(s) 30, 274
MroXI GAANNNNTTC 1 cut(s) 170
MseI TTAA 2 cut(s) 89, 352
MspA1I CMGCKG 1 cut(s) 233
MunI CAATTG 1 cut(s) 59
NdeII GATC 3 cut(s) 63, 227, 309
NlaIII CATG 2 cut(s) 178, 203
NmuCI GTSAC 1 cut(s) 115
PdmI GAANNNNTTC 1 cut(s) 170
PkrI GCNGC 2 cut(s) 219, 222
Psp124BI GAGCTC 1 cut(s) 73
PspPI GGNCC 1 cut(s) 318
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 2 cut(s) 81, 302
RsaNI GTAC 2 cut(s) 80, 301
SacI GAGCTC 1 cut(s) 73
SaqAI TTAA 2 cut(s) 89, 352
SatI GCNGC 2 cut(s) 218, 221
Sau3AI GATC 3 cut(s) 63, 227, 309
Sau96I GGNCC 1 cut(s) 318
ScaI AGTACT 1 cut(s) 302
SduI GDGCHC 1 cut(s) 73
SetI ASST 8 cut(s) 20, 51, 73, 130, 191, 219, 235, 342
SfaNI GCATC 1 cut(s) 51
SgeI CNNG 9 cut(s) 58, 87, 149, 187, 212, 292, 298, 316, 349
SinI GGWCC 1 cut(s) 318
Sse9I AATT 5 cut(s) 59, 109, 206, 270, 330
SstI GAGCTC 1 cut(s) 73
StyI CCWWGG 1 cut(s) 285
TaaI ACNGT 1 cut(s) 318
TaiI ACGT 1 cut(s) 191
TasI AATT 5 cut(s) 59, 109, 206, 270, 330
TatI WGTACW 2 cut(s) 79, 300
Tru1I TTAA 2 cut(s) 89, 352
Tru9I TTAA 2 cut(s) 89, 352
TscAI CASTG 1 cut(s) 120
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 2 cut(s) 217, 220
Tsp45I GTSAC 1 cut(s) 115
TspDTI ATGAA 4 cut(s) 17, 163, 170, 185
TspRI CASTG 1 cut(s) 120
VpaK11BI GGWCC 1 cut(s) 318
XapI RAATTY 3 cut(s) 206, 270, 330
XmnI GAANNNNTTC 1 cut(s) 170
ZrmI AGTACT 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.