pycom09g10770

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0010585
Physical Location & Seq
Forward (+)
8863709 .. 8864041
333 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g10770.1

Sequence Viewer

Length: 333 bp
ATGAAGAGAGTACTTTGCCATTTGCTAAAGGGGGTGAGAAGCAGCAGGTACAGCCAGTTAATCGGAGCTCGAGCAGCAAAAATACTGATAAGCCTTTCTCCTTTAACACCAAAAGGGTATGTGCCTGTTTGTGTTGGTGTGGATGGTGATACGAAGCGTTTCATGGTTCATACCACGTTGCTTGGCCATGCAGAATTCTTGGAGCTGCTGCATAAATCAGCTGAAGAGTATGGTTTTTGCAATGACAGTGTTTTGAGAATTCCATATGAAGCGCAGGATTTTGAGGAGTATTGGATCGATCAAGAGGTCAAAAGCAAGGATTTACAAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

111

Amino Acids

12.46

Weight (kDa)

6.89

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 35 - 96 8.9e-16 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 36
AclWI GGATC 1 cut(s) 302
AcoI YGGCCR 1 cut(s) 184
AcsI RAATTY 2 cut(s) 194, 258
AcuI CTGAAG 1 cut(s) 243
AfaI GTAC 2 cut(s) 12, 50
AluBI AGCT 3 cut(s) 68, 205, 221
AluI AGCT 3 cut(s) 68, 205, 221
Alw21I GWGCWC 1 cut(s) 70
AlwI GGATC 1 cut(s) 302
Ama87I CYCGRG 1 cut(s) 69
AoxI GGCC 1 cut(s) 184
ApeKI GCWGC 4 cut(s) 42, 74, 205, 208
ApoI RAATTY 2 cut(s) 194, 258
Asp700I GAANNNNTTC 1 cut(s) 158
AspLEI GCGC 1 cut(s) 274
AsuHPI GGTGA 2 cut(s) 46, 158
AvaI CYCGRG 1 cut(s) 69
BalI TGGCCA 1 cut(s) 186
BanII GRGCYC 1 cut(s) 70
Bbv12I GWGCWC 1 cut(s) 70
BbvI GCAGC 4 cut(s) 54, 86, 192, 195
BccI CCATC 1 cut(s) 137
BfuAI ACCTGC 1 cut(s) 36
BisI GCNGC 4 cut(s) 43, 75, 206, 209
BlsI GCNGC 4 cut(s) 44, 76, 207, 210
BmcAI AGTACT 1 cut(s) 12
BmeT110I CYCGRG 1 cut(s) 69
Bsa29I ATCGAT 1 cut(s) 297
Bse1I ACTGG 1 cut(s) 55
Bse3DI GCAATG 1 cut(s) 247
BseCI ATCGAT 1 cut(s) 297
BseGI GGATG 1 cut(s) 148
BseMI GCAATG 1 cut(s) 247
BseNI ACTGG 1 cut(s) 55
BseRI GAGGAG 1 cut(s) 299
BseXI GCAGC 4 cut(s) 54, 86, 192, 195
BshFI GGCC 1 cut(s) 186
BshVI ATCGAT 1 cut(s) 297
BsiHKAI GWGCWC 1 cut(s) 70
BsiHKCI CYCGRG 1 cut(s) 69
BsnI GGCC 1 cut(s) 186
BsoBI CYCGRG 1 cut(s) 69
Bsp1286I GDGCHC 1 cut(s) 70
Bsp143I GATC 2 cut(s) 294, 298
BspANI GGCC 1 cut(s) 186
BspDI ATCGAT 1 cut(s) 297
BspMI ACCTGC 1 cut(s) 36
BspPI GGATC 1 cut(s) 302
BsrDI GCAATG 1 cut(s) 247
BsrI ACTGG 1 cut(s) 55
BssMI GATC 2 cut(s) 294, 298
Bst4CI ACNGT 1 cut(s) 248
Bst6I CTCTTC 1 cut(s) 219
BstF5I GGATG 1 cut(s) 148
BstHHI GCGC 1 cut(s) 274
BstKTI GATC 2 cut(s) 297, 301
BstMBI GATC 2 cut(s) 294, 298
BstMWI GCNNNNNNNGC 2 cut(s) 51, 74
BstV1I GCAGC 4 cut(s) 54, 86, 192, 195
Bsu15I ATCGAT 1 cut(s) 297
BsuRI GGCC 1 cut(s) 186
BsuTUI ATCGAT 1 cut(s) 297
BtsCI GGATG 1 cut(s) 148
BtsIMutI CAGTG 1 cut(s) 253
BveI ACCTGC 1 cut(s) 36
CfoI GCGC 1 cut(s) 274
ClaI ATCGAT 1 cut(s) 297
Csp6I GTAC 2 cut(s) 11, 49
CspCI CAANNNNNGTGG 2 cut(s) 163, 198
CviAII CATG 2 cut(s) 163, 188
CviJI RGCY 6 cut(s) 54, 68, 93, 186, 205, 221
CviKI_1 RGCY 6 cut(s) 54, 68, 93, 186, 205, 221
CviQI GTAC 2 cut(s) 11, 49
DpnI GATC 2 cut(s) 296, 300
DpnII GATC 2 cut(s) 294, 298
EaeI YGGCCR 1 cut(s) 184
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Ecl136II GAGCTC 1 cut(s) 68
Eco24I GRGCYC 1 cut(s) 70
Eco53kI GAGCTC 1 cut(s) 68
Eco57I CTGAAG 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 69
EcoICRI GAGCTC 1 cut(s) 68
EcoRI GAATTC 2 cut(s) 194, 258
EcoT38I GRGCYC 1 cut(s) 70
FaeI CATG 2 cut(s) 166, 191
FaiI YATR 8 cut(s) 120, 164, 171, 189, 213, 231, 265, 267
FatI CATG 2 cut(s) 162, 187
FauNDI CATATG 1 cut(s) 265
Fnu4HI GCNGC 4 cut(s) 43, 75, 206, 209
FokI GGATG 1 cut(s) 155
FriOI GRGCYC 1 cut(s) 70
Fsp4HI GCNGC 4 cut(s) 43, 75, 206, 209
GlaI GCGC 1 cut(s) 273
GluI GCNGC 4 cut(s) 43, 75, 206, 209
HaeIII GGCC 1 cut(s) 186
HhaI GCGC 1 cut(s) 274
Hin1II CATG 2 cut(s) 166, 191
Hin6I GCGC 1 cut(s) 272
HinP1I GCGC 1 cut(s) 272
HphI GGTGA 2 cut(s) 46, 158
Hpy188I TCNGA 1 cut(s) 65
Hpy188III TCNNGA 1 cut(s) 302
HpyCH4III ACNGT 1 cut(s) 248
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 3 cut(s) 191, 211, 240
HpyF10VI GCNNNNNNNGC 2 cut(s) 51, 74
HpySE526I ACGT 1 cut(s) 176
Hsp92II CATG 2 cut(s) 166, 191
HspAI GCGC 1 cut(s) 272
Kzo9I GATC 2 cut(s) 294, 298
LmnI GCTCC 2 cut(s) 65, 202
LpnPI CCDG 4 cut(s) 31, 68, 138, 260
Lsp1109I GCAGC 4 cut(s) 54, 86, 192, 195
MaeII ACGT 1 cut(s) 176
MalI GATC 2 cut(s) 296, 300
MboI GATC 2 cut(s) 294, 298
MboII GAAGA 2 cut(s) 16, 236
MhlI GDGCHC 1 cut(s) 70
MlsI TGGCCA 1 cut(s) 186
MluCI AATT 2 cut(s) 194, 258
MluNI TGGCCA 1 cut(s) 186
MnlI CCTC 2 cut(s) 277, 298
Mox20I TGGCCA 1 cut(s) 186
MroXI GAANNNNTTC 1 cut(s) 158
MscI TGGCCA 1 cut(s) 186
MseI TTAA 2 cut(s) 59, 104
Msp20I TGGCCA 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 221
MwoI GCNNNNNNNGC 2 cut(s) 51, 74
NdeI CATATG 1 cut(s) 265
NdeII GATC 2 cut(s) 294, 298
NlaIII CATG 2 cut(s) 166, 191
PaeR7I CTCGAG 1 cut(s) 69
PdmI GAANNNNTTC 1 cut(s) 158
PkrI GCNGC 4 cut(s) 44, 76, 207, 210
Psp124BI GAGCTC 1 cut(s) 70
PspXI VCTCGAGB 1 cut(s) 69
PvuII CAGCTG 1 cut(s) 221
RsaI GTAC 2 cut(s) 12, 50
RsaNI GTAC 2 cut(s) 11, 49
SacI GAGCTC 1 cut(s) 70
SaqAI TTAA 2 cut(s) 59, 104
SatI GCNGC 4 cut(s) 43, 75, 206, 209
Sau3AI GATC 2 cut(s) 294, 298
ScaI AGTACT 1 cut(s) 12
SduI GDGCHC 1 cut(s) 70
SetI ASST 7 cut(s) 50, 70, 179, 207, 223, 309, 331
Sfr274I CTCGAG 1 cut(s) 69
SlaI CTCGAG 1 cut(s) 69
SmlI CTYRAG 1 cut(s) 69
SmoI CTYRAG 1 cut(s) 69
Sse9I AATT 2 cut(s) 194, 258
SstI GAGCTC 1 cut(s) 70
TaaI ACNGT 1 cut(s) 248
TaiI ACGT 1 cut(s) 179
TaqI TCGA 2 cut(s) 70, 297
TasI AATT 2 cut(s) 194, 258
TatI WGTACW 1 cut(s) 10
Tru1I TTAA 2 cut(s) 59, 104
Tru9I TTAA 2 cut(s) 59, 104
TscAI CASTG 1 cut(s) 253
TseI GCWGC 4 cut(s) 42, 74, 205, 208
TspDTI ATGAA 4 cut(s) 17, 151, 158, 282
TspRI CASTG 1 cut(s) 253
XapI RAATTY 2 cut(s) 194, 258
XhoI CTCGAG 1 cut(s) 69
XmnI GAANNNNTTC 1 cut(s) 158
ZrmI AGTACT 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.