Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0004596

Physical Location & Seq

Genomic Coordinates Forward (+)

17451281 .. 17457326

Gene Structure 6046 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1194200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1518 bp

ATGAGCCGCTACGACAGCCGCTCCGGCGACCCCACTTCCTACCGTGACCGGAGAAGTGATTCCGGTTTTGGTGGGGCAACCGGCTATGGTGGCTCAGCACGGTCTTCGTCAAGCAGGAGGGATAATGATGGTGCTGAGTCACCAAGGAAGTTGGATTTGGATGGATTGATACCTTTTGAGAAGAATTTCTATGTTGAGTCGGCTGAAGTGGAAAAAATGTCAGAGGTGGAGGTTGAGGAGTATAGGAAACGGAGGGAAATTACTGTTGAAGGCCGGGATGTTCCAAAACCCATCAAGAGTTTCCGTGACACTGGGTTTCCAGAATACGTTTTGGAAGAAGTTACAAAGGCTGGATTTGTGGAACCTACACCAATCCAAGCTCAAGGATGGCCAATGGCTTTGAAAGGTCGTGATCTCATTGGTATTGCTGAAACAGGATCAGGGAAGACACTAGCCTACCTGTTGCCTGCAATCGTCCATGTCAATGCCCAGCCAATTCTATCTCCTGGAGATGGCCCGATAGTTCTAGTTTTAGCTCCAACCCGTGAACTTGCTGTTCAAATCCAACAAGAGGCTACTAAATTTGGTTCATCGTCAAAAATTAAAAGCACATGCATATATGGTGGGGTTCCAAAGGGACCTCAAGTTCGTGATCTGCAGAAAGGAGTTGAGATTGTAATTGCTACACCAGGAAGATTGATTGATATGATGGAATCACATCATACCAACTTGCGAAGGGTCACTTACCTTGTATTAGATGAAGCTGATCGGATGCTAGACATGGGGTTTGACCCTCAAATAAGGAAGATTGTTTCACAGATTCGCCCAGACCGTCAAACATTATACTGGAGTGCTACCTGGCCAAAGGAGGTTGAACAACTGGCGAGGCAGTTCCTTTACAACCCATACAAAGTGATAATAGGCTCTCCAGATTTGAAAGCTAACCATTCGATCCGCCAAATCGTTGACATCATTACTGAAAATCAGAAATATAACAAATTGGTAAAGTTGCTCGAGGATATCATGGATGGCAGCAGAATATTGATATTCATGGATACTAAGAAAGGATGTGATCAGATCACTCGGCAGCTCCGCATGGATGGTTGGCCTGCTCTTTCGATTCATGGTGACAAGAGTCAAGCAGAAAGGGATTGGGTCCTCTCCGAGTTTAAAGCGGGCAAGAGCCCCATAATGACTGCAACAGATGTTGCTGCTCGTGGTCTAGATGTGAAGGATGTGAAATTTGTTATTAACTATGATTTTCCTGGATCCCTTGAGGATTATGTTCACCGCATTGGCCGAACCGGAAGAGCTGGGGCAAAAGGAACAGCTTACACATTCTTTACAGCTGCTAATGCTAGGTTCGCAAAGGAACTTATTGCCTTGCTTGAGGAAGCTGGACAGAAAGTCAGTCCTGAGTTGGCAGCAATGGGTCGTGGTGCACCACCTCCCCCTTCAGGTCATGGGGGCTTTCGAGATCGTGGTAGGGGCTATGGAGGCGGTCGTCCTTGGAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03040 ko05202 ko05205 map03040 map05202 map05205

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

506

Amino Acids

56.01

Weight (kDa)

8.85

Isoelectric Point (pI)

39.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	123 - 293	3.2e-50	DEAD/DEAH box helicase
ResIII	PF04851	142 - 286	1.2e-06	Type III restriction enzyme, res subunit
Helicase_C	PF00271	330 - 439	1.4e-31	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004596)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55150 AT1G55150
fragaria_vesca	FvH4_6g33220 FvH4_6g33220 FvH4_6g33220 FvH4_6g33220 FvH4_6g33220 FvH4_6g33220 FvH4_6g33220
malus_domestica	MD09G1194200.v1.1 MD17G1174700.v1.1
prunus_persica	Prupe.3G019300_v2.0.a1 Prupe.3G019300_v2.0.a1 Prupe.3G050700_v2.0.a1 Prupe.3G050700_v2.0.a1 Prupe.3G050700_v2.0.a1
pyrus_communis	pycom09g11070
rosa_chinensis	RchiOBHm_Chr2g0142931
rosa_laevigata	RLG00000019997
rosa_multiflora	Rmu_sc0028648.1_g000001
rosa_roxburghii	Rroxscaffold_2G00102220 Rroxscaffold_2G00103260
rosa_rugosa	Rorug02G0377000 Rorug02G0377100 Rorug02G0377200
rosa_samantha	Rh2AG430000 Rh2BG439000 Rh2CG362000 Rh2CG415500 Rh2DG449000
rosa_wichuraiana	Rw2G035130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	21
AciI	CCGC	7 cut(s)	7, 19, 955, 1093, 1175, 1291, 1500
AclWI	GGATC	4 cut(s)	445, 946, 1263, 1276
AcoI	YGGCCR	3 cut(s)	389, 860, 1297
AcsI	RAATTY	3 cut(s)	184, 581, 1241
AcuI	CTGAAG	2 cut(s)	225, 1440
AfiI	CCNNNNNNNGG	3 cut(s)	512, 571, 1457
AgsI	TTSAA	5 cut(s)	269, 403, 560, 875, 937
AhdI	GACNNNNNGTC	1 cut(s)	1406
AjnI	CCWGG	4 cut(s)	505, 688, 857, 1264
AjuI	GAANNNNNNNTTGG	2 cut(s)	140, 172
AloI	GAACNNNNNNTCC	2 cut(s)	630, 662
AluBI	AGCT	9 cut(s)	380, 536, 764, 941, 1090, 1313, 1331, 1349, 1397
AluI	AGCT	9 cut(s)	380, 536, 764, 941, 1090, 1313, 1331, 1349, 1397
Alw21I	GWGCWC	1 cut(s)	1444
Alw44I	GTGCAC	1 cut(s)	1440
AlwI	GGATC	4 cut(s)	445, 946, 1263, 1276
Ama87I	CYCGRG	1 cut(s)	1013
AoxI	GGCC	6 cut(s)	271, 389, 514, 860, 1106, 1297
ApaLI	GTGCAC	1 cut(s)	1440
ApeKI	GCWGC	5 cut(s)	1032, 1087, 1211, 1349, 1424
ApoI	RAATTY	3 cut(s)	184, 581, 1241
ArsI	GACNNNNNNTTYG	4 cut(s)	630, 662, 770, 802
Asp700I	GAANNNNTTC	2 cut(s)	58, 185
AspS9I	GGNCC	3 cut(s)	515, 638, 1156
AsuC2I	CCSGG	1 cut(s)	275
AsuHPI	GGTGA	3 cut(s)	132, 1139, 1280
AvaI	CYCGRG	1 cut(s)	1013
AvaII	GGWCC	2 cut(s)	638, 1156
BaeGI	GKGCMC	1 cut(s)	1444
BalI	TGGCCA	2 cut(s)	391, 862
BamHI	GGATCC	1 cut(s)	1268
BanII	GRGCYC	1 cut(s)	1187
BauI	CACGAG	1 cut(s)	1215
BbsI	GAAGAC	2 cut(s)	96, 452
Bbv12I	GWGCWC	1 cut(s)	1444
BbvI	GCAGC	5 cut(s)	1044, 1099, 1198, 1336, 1436
BccI	CCATC	8 cut(s)	122, 155, 299, 381, 506, 703, 1022, 1094
BcgI	CGANNNNNNTGC	2 cut(s)	87, 121
BciT130I	CCWGG	4 cut(s)	507, 690, 859, 1266
BciVI	GTATCC	1 cut(s)	1048
BclI	TGATCA	1 cut(s)	1072
BcnI	CCSGG	1 cut(s)	275
BfaI	CTAG	5 cut(s)	452, 527, 776, 1223, 1359
BfmI	CTRYAG	1 cut(s)	656
BfuI	GTATCC	1 cut(s)	1048
BglI	GCCNNNNNGGC	1 cut(s)	24
BisI	GCNGC	7 cut(s)	7, 19, 1033, 1088, 1212, 1350, 1425
BlpI	GCTNAGC	1 cut(s)	94
BlsI	GCNGC	7 cut(s)	8, 20, 1034, 1089, 1213, 1351, 1426
Bme1390I	CCNGG	5 cut(s)	275, 507, 690, 859, 1266
Bme18I	GGWCC	2 cut(s)	638, 1156
BmeRI	GACNNNNNGTC	1 cut(s)	1406
BmeT110I	CYCGRG	1 cut(s)	1013
BmgT120I	GGNCC	3 cut(s)	515, 638, 1156
BmiI	GGNNCC	5 cut(s)	363, 630, 639, 1157, 1270
BmrFI	CCNGG	5 cut(s)	275, 507, 690, 859, 1266
BmrI	ACTGGG	1 cut(s)	321
BmsI	GCATC	1 cut(s)	762
BmuI	ACTGGG	1 cut(s)	321
BoxI	GACNNNNGTC	1 cut(s)	1134
BpiI	GAAGAC	2 cut(s)	96, 452
BpmI	CTGGAG	3 cut(s)	528, 868, 912
Bpu1102I	GCTNAGC	1 cut(s)	94
BpuEI	CTTGAG	4 cut(s)	366, 627, 1295, 1409
BpuMI	CCSGG	1 cut(s)	275
BsaJI	CCNNGG	2 cut(s)	143, 1508
BsaWI	WCCGGW	3 cut(s)	48, 62, 1304
BsaXI	ACNNNNNCTCC	2 cut(s)	5, 35
Bsc4I	CCNNNNNNNGG	3 cut(s)	512, 571, 1457
Bse118I	RCCGGY	1 cut(s)	80
Bse1I	ACTGG	3 cut(s)	316, 851, 885
Bse3DI	GCAATG	1 cut(s)	1434
BseBI	CCWGG	4 cut(s)	507, 690, 859, 1266
BseDI	CCNNGG	2 cut(s)	143, 1508
BseGI	GGATG	8 cut(s)	166, 283, 392, 777, 1033, 1073, 1105, 1240
BseLI	CCNNNNNNNGG	3 cut(s)	512, 571, 1457
BseMI	GCAATG	1 cut(s)	1434
BseMII	CTCAG	3 cut(s)	108, 126, 1407
BseNI	ACTGG	3 cut(s)	316, 851, 885
BseRI	GAGGAG	1 cut(s)	251
BseSI	GKGCMC	1 cut(s)	1444
BseXI	GCAGC	5 cut(s)	1044, 1099, 1198, 1336, 1436
BseYI	CCCAGC	2 cut(s)	489, 1313
Bsh1285I	CGRYCG	1 cut(s)	1504
BshFI	GGCC	6 cut(s)	273, 391, 516, 862, 1108, 1299
BsiEI	CGRYCG	1 cut(s)	1504
BsiHKAI	GWGCWC	1 cut(s)	1444
BsiHKCI	CYCGRG	1 cut(s)	1013
BsiSI	CCGG	6 cut(s)	24, 49, 63, 81, 274, 1305
BslFI	GGGAC	1 cut(s)	651
BslI	CCNNNNNNNGG	3 cut(s)	512, 571, 1457
BsmFI	GGGAC	1 cut(s)	651
BsnI	GGCC	6 cut(s)	273, 391, 516, 862, 1108, 1299
BsoBI	CYCGRG	1 cut(s)	1013
Bsp1286I	GDGCHC	2 cut(s)	1187, 1444
Bsp143I	GATC	9 cut(s)	412, 437, 652, 766, 951, 1072, 1077, 1268, 1477
Bsp1720I	GCTNAGC	1 cut(s)	94
BspACI	CCGC	7 cut(s)	7, 19, 955, 1093, 1175, 1291, 1500
BspANI	GGCC	6 cut(s)	273, 391, 516, 862, 1108, 1299
BspCNI	CTCAG	3 cut(s)	107, 127, 1408
BspLI	GGNNCC	5 cut(s)	363, 630, 639, 1157, 1270
BspMAI	CTGCAG	1 cut(s)	660
BspPI	GGATC	4 cut(s)	445, 946, 1263, 1276
BspQI	GCTCTTC	1 cut(s)	1303
BsrBI	CCGCTC	1 cut(s)	21
BsrDI	GCAATG	1 cut(s)	1434
BsrFI	RCCGGY	1 cut(s)	80
BsrI	ACTGG	3 cut(s)	316, 851, 885
BssAI	RCCGGY	1 cut(s)	80
BssECI	CCNNGG	2 cut(s)	143, 1508
BssMI	GATC	9 cut(s)	412, 437, 652, 766, 951, 1072, 1077, 1268, 1477
BssSI	CACGAG	1 cut(s)	1215
BssT1I	CCWWGG	2 cut(s)	143, 1508
Bst2BI	CACGAG	1 cut(s)	1215
Bst2UI	CCWGG	4 cut(s)	507, 690, 859, 1266
Bst4CI	ACNGT	4 cut(s)	44, 102, 265, 833
Bst6I	CTCTTC	1 cut(s)	1303
BstC8I	GCNNGC	3 cut(s)	468, 1110, 1177
BstDEI	CTNAG	4 cut(s)	94, 135, 1059, 1416
BstF5I	GGATG	8 cut(s)	166, 283, 392, 777, 1033, 1073, 1105, 1240
BstKTI	GATC	9 cut(s)	415, 440, 655, 769, 954, 1075, 1080, 1271, 1480
BstMBI	GATC	9 cut(s)	412, 437, 652, 766, 951, 1072, 1077, 1268, 1477
BstMCI	CGRYCG	1 cut(s)	1504
BstMWI	GCNNNNNNNGC	6 cut(s)	15, 24, 90, 1355, 1364, 1497
BstNI	CCWGG	4 cut(s)	507, 690, 859, 1266
BstNSI	RCATGY	1 cut(s)	615
BstPAI	GACNNNNGTC	1 cut(s)	1134
BstSCI	CCNGG	5 cut(s)	273, 505, 688, 857, 1264
BstSFI	CTRYAG	1 cut(s)	656
BstSLI	GKGCMC	1 cut(s)	1444
BstV1I	GCAGC	5 cut(s)	1044, 1099, 1198, 1336, 1436
BstV2I	GAAGAC	2 cut(s)	96, 452
BstX2I	RGATCY	1 cut(s)	1268
BstYI	RGATCY	1 cut(s)	1268
BsuI	GTATCC	1 cut(s)	1048
BsuRI	GGCC	6 cut(s)	273, 391, 516, 862, 1108, 1299
BtsCI	GGATG	8 cut(s)	166, 283, 392, 777, 1033, 1073, 1105, 1240
BtsIMutI	CAGTG	1 cut(s)	309
Cac8I	GCNNGC	3 cut(s)	468, 1110, 1177
Cfr10I	RCCGGY	1 cut(s)	80
Cfr13I	GGNCC	3 cut(s)	515, 638, 1156
CviAII	CATG	8 cut(s)	479, 612, 781, 1024, 1051, 1096, 1124, 1463
DdeI	CTNAG	4 cut(s)	94, 135, 1059, 1416
DpnI	GATC	9 cut(s)	414, 439, 654, 768, 953, 1074, 1079, 1270, 1479
DpnII	GATC	9 cut(s)	412, 437, 652, 766, 951, 1072, 1077, 1268, 1477
DraI	TTTAAA	1 cut(s)	1171
DriI	GACNNNNNGTC	1 cut(s)	1406
EaeI	YGGCCR	3 cut(s)	389, 860, 1297
Eam1104I	CTCTTC	1 cut(s)	1303
Eam1105I	GACNNNNNGTC	1 cut(s)	1406
EarI	CTCTTC	1 cut(s)	1303
EciI	GGCGGA	1 cut(s)	944
Eco130I	CCWWGG	2 cut(s)	143, 1508
Eco24I	GRGCYC	1 cut(s)	1187
Eco32I	GATATC	1 cut(s)	1021
Eco47I	GGWCC	2 cut(s)	638, 1156
Eco57I	CTGAAG	2 cut(s)	225, 1440
Eco88I	CYCGRG	1 cut(s)	1013
EcoO109I	RGGNCCY	2 cut(s)	638, 1156
EcoRII	CCWGG	4 cut(s)	505, 688, 857, 1264
EcoRV	GATATC	1 cut(s)	1021
EcoT14I	CCWWGG	2 cut(s)	143, 1508
EcoT22I	ATGCAT	1 cut(s)	617
EcoT38I	GRGCYC	1 cut(s)	1187
ErhI	CCWWGG	2 cut(s)	143, 1508
FaeI	CATG	8 cut(s)	482, 615, 784, 1027, 1054, 1099, 1127, 1466
FalI	AAGNNNNNCTT	2 cut(s)	727, 759
FaqI	GGGAC	1 cut(s)	651
FatI	CATG	8 cut(s)	478, 611, 780, 1023, 1050, 1095, 1123, 1462
FauI	CCCGC	1 cut(s)	1168
FbaI	TGATCA	1 cut(s)	1072
Fnu4HI	GCNGC	7 cut(s)	7, 19, 1033, 1088, 1212, 1350, 1425
FokI	GGATG	8 cut(s)	173, 290, 399, 784, 1040, 1080, 1112, 1247
FriOI	GRGCYC	1 cut(s)	1187
Fsp4HI	GCNGC	7 cut(s)	7, 19, 1033, 1088, 1212, 1350, 1425
FspBI	CTAG	5 cut(s)	452, 527, 776, 1223, 1359
GluI	GCNGC	7 cut(s)	7, 19, 1033, 1088, 1212, 1350, 1425
GsaI	CCCAGC	2 cut(s)	493, 1317
GsuI	CTGGAG	3 cut(s)	528, 868, 912
HaeIII	GGCC	6 cut(s)	273, 391, 516, 862, 1108, 1299
HapII	CCGG	6 cut(s)	24, 49, 63, 81, 274, 1305
Hin1II	CATG	8 cut(s)	482, 615, 784, 1027, 1054, 1099, 1127, 1466
HincII	GTYRAC	1 cut(s)	967
HindII	GTYRAC	1 cut(s)	967
HinfI	GANTC	7 cut(s)	59, 137, 197, 713, 820, 1120, 1135
HpaII	CCGG	6 cut(s)	24, 49, 63, 81, 274, 1305
HphI	GGTGA	3 cut(s)	132, 1139, 1280
Hpy166II	GTNNAC	4 cut(s)	548, 967, 1288, 1442
Hpy188I	TCNGA	5 cut(s)	223, 771, 987, 1077, 1165
Hpy188III	TCNNGA	8 cut(s)	295, 320, 410, 650, 929, 1223, 1415, 1475
Hpy8I	GTNNAC	4 cut(s)	548, 967, 1288, 1442
HpyAV	CCTTC	4 cut(s)	263, 729, 1225, 1464
HpyCH4III	ACNGT	4 cut(s)	44, 102, 265, 833
HpyCH4IV	ACGT	1 cut(s)	327
HpyCH4V	TGCA	5 cut(s)	470, 615, 658, 1199, 1442
HpyF10VI	GCNNNNNNNGC	6 cut(s)	15, 24, 90, 1355, 1364, 1497
HpyF3I	CTNAG	4 cut(s)	94, 135, 1059, 1416
HpySE526I	ACGT	1 cut(s)	327
Hsp92II	CATG	8 cut(s)	482, 615, 784, 1027, 1054, 1099, 1127, 1466
Ksp22I	TGATCA	1 cut(s)	1072
Kzo9I	GATC	9 cut(s)	412, 437, 652, 766, 951, 1072, 1077, 1268, 1477
LguI	GCTCTTC	1 cut(s)	1303
LmnI	GCTCC	3 cut(s)	26, 541, 1095
Lsp1109I	GCAGC	5 cut(s)	1044, 1099, 1198, 1336, 1436
LweI	GCATC	1 cut(s)	762
MaeI	CTAG	5 cut(s)	452, 527, 776, 1223, 1359
MaeII	ACGT	1 cut(s)	327
MaeIII	GTNAC	6 cut(s)	44, 138, 305, 340, 739, 1127
MalI	GATC	9 cut(s)	414, 439, 654, 768, 953, 1074, 1079, 1270, 1479
MbiI	CCGCTC	1 cut(s)	21
MboI	GATC	9 cut(s)	412, 437, 652, 766, 951, 1072, 1077, 1268, 1477
MboII	GAAGA	7 cut(s)	96, 193, 347, 457, 705, 817, 1320
MflI	RGATCY	1 cut(s)	1268
MhlI	GDGCHC	2 cut(s)	1187, 1444
MlsI	TGGCCA	2 cut(s)	391, 862
MluCI	AATT	8 cut(s)	184, 258, 495, 581, 600, 678, 998, 1241
MluNI	TGGCCA	2 cut(s)	391, 862
MlyI	GAGTC	3 cut(s)	146, 206, 1144
MmeI	TCCRAC	3 cut(s)	132, 563, 589
Mox20I	TGGCCA	2 cut(s)	391, 862
Mph1103I	ATGCAT	1 cut(s)	617
MroXI	GAANNNNTTC	2 cut(s)	58, 185
MscI	TGGCCA	2 cut(s)	391, 862
MseI	TTAA	3 cut(s)	603, 1170, 1251
MslI	CAYNNNNRTG	1 cut(s)	483
Msp20I	TGGCCA	2 cut(s)	391, 862
MspA1I	CMGCKG	1 cut(s)	1349
MspI	CCGG	6 cut(s)	24, 49, 63, 81, 274, 1305
MspR9I	CCNGG	5 cut(s)	275, 507, 690, 859, 1266
MvaI	CCWGG	4 cut(s)	507, 690, 859, 1266
MwoI	GCNNNNNNNGC	6 cut(s)	15, 24, 90, 1355, 1364, 1497
NciI	CCSGG	1 cut(s)	275
NdeII	GATC	9 cut(s)	412, 437, 652, 766, 951, 1072, 1077, 1268, 1477
NlaIII	CATG	8 cut(s)	482, 615, 784, 1027, 1054, 1099, 1127, 1466
NlaIV	GGNNCC	5 cut(s)	363, 630, 639, 1157, 1270
NmeAIII	GCCGAG	1 cut(s)	1063
NmuCI	GTSAC	5 cut(s)	44, 138, 305, 739, 1127
NsiI	ATGCAT	1 cut(s)	617
NspI	RCATGY	1 cut(s)	615
PaeR7I	CTCGAG	1 cut(s)	1013
PciSI	GCTCTTC	1 cut(s)	1303
PcsI	WCGNNNNNNNCGW	1 cut(s)	829
PdmI	GAANNNNTTC	2 cut(s)	58, 185
PfeI	GAWTC	4 cut(s)	59, 713, 820, 1120
PfoI	TCCNGGA	2 cut(s)	505, 1264
PkrI	GCNGC	7 cut(s)	8, 20, 1034, 1089, 1213, 1351, 1426
PleI	GAGTC	3 cut(s)	145, 205, 1143
PpsI	GAGTC	3 cut(s)	145, 205, 1143
PpuMI	RGGWCCY	2 cut(s)	638, 1156
PshAI	GACNNNNGTC	1 cut(s)	1134
Psp5II	RGGWCCY	2 cut(s)	638, 1156
Psp6I	CCWGG	4 cut(s)	505, 688, 857, 1264
PspFI	CCCAGC	2 cut(s)	489, 1313
PspGI	CCWGG	4 cut(s)	505, 688, 857, 1264
PspN4I	GGNNCC	5 cut(s)	363, 630, 639, 1157, 1270
PspPI	GGNCC	3 cut(s)	515, 638, 1156
PspPPI	RGGWCCY	2 cut(s)	638, 1156
PspXI	VCTCGAGB	1 cut(s)	1013
PstI	CTGCAG	1 cut(s)	660
PsuI	RGATCY	1 cut(s)	1268
PvuII	CAGCTG	1 cut(s)	1349
RseI	CAYNNNNRTG	1 cut(s)	483
SapI	GCTCTTC	1 cut(s)	1303
SaqAI	TTAA	3 cut(s)	603, 1170, 1251
SatI	GCNGC	7 cut(s)	7, 19, 1033, 1088, 1212, 1350, 1425
Sau3AI	GATC	9 cut(s)	412, 437, 652, 766, 951, 1072, 1077, 1268, 1477
Sau96I	GGNCC	3 cut(s)	515, 638, 1156
SchI	GAGTC	3 cut(s)	146, 206, 1144
ScrFI	CCNGG	5 cut(s)	275, 507, 690, 859, 1266
SduI	GDGCHC	2 cut(s)	1187, 1444
SfaNI	GCATC	1 cut(s)	762
SfcI	CTRYAG	1 cut(s)	656
Sfr274I	CTCGAG	1 cut(s)	1013
SinI	GGWCC	2 cut(s)	638, 1156
SlaI	CTCGAG	1 cut(s)	1013
SmiMI	CAYNNNNRTG	1 cut(s)	483
SmlI	CTYRAG	5 cut(s)	381, 642, 1013, 1274, 1388
SmoI	CTYRAG	5 cut(s)	381, 642, 1013, 1274, 1388
Sse9I	AATT	8 cut(s)	184, 258, 495, 581, 600, 678, 998, 1241
SsiI	CCGC	7 cut(s)	7, 19, 955, 1093, 1175, 1291, 1500
SspI	AATATT	1 cut(s)	1041
SspMI	CTAG	5 cut(s)	452, 527, 776, 1223, 1359
StyD4I	CCNGG	5 cut(s)	273, 505, 688, 857, 1264
StyI	CCWWGG	2 cut(s)	143, 1508
TaaI	ACNGT	4 cut(s)	44, 102, 265, 833
TaiI	ACGT	1 cut(s)	330
TaqI	TCGA	4 cut(s)	950, 1014, 1118, 1474
TasI	AATT	8 cut(s)	184, 258, 495, 581, 600, 678, 998, 1241
TauI	GCSGC	2 cut(s)	9, 21
TfiI	GAWTC	4 cut(s)	59, 713, 820, 1120
Tru1I	TTAA	3 cut(s)	603, 1170, 1251
Tru9I	TTAA	3 cut(s)	603, 1170, 1251
TscAI	CASTG	1 cut(s)	316
TseFI	GTSAC	5 cut(s)	44, 138, 305, 739, 1127
TseI	GCWGC	5 cut(s)	1032, 1087, 1211, 1349, 1424
Tsp45I	GTSAC	5 cut(s)	44, 138, 305, 739, 1127
TspDTI	ATGAA	4 cut(s)	579, 774, 1039, 1112
TspGWI	ACGGA	2 cut(s)	265, 293
TspRI	CASTG	1 cut(s)	316
VneI	GTGCAC	1 cut(s)	1440
VpaK11BI	GGWCC	2 cut(s)	638, 1156
XapI	RAATTY	3 cut(s)	184, 581, 1241
XbaI	TCTAGA	1 cut(s)	1222
XceI	RCATGY	1 cut(s)	615
XhoI	CTCGAG	1 cut(s)	1013
XmnI	GAANNNNTTC	2 cut(s)	58, 185
XspI	CTAG	5 cut(s)	452, 527, 776, 1223, 1359
Zsp2I	ATGCAT	1 cut(s)	617

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.