Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004596

Physical Location & Seq

Genomic Coordinates Forward (+)

19505667 .. 19511108

Gene Structure 5442 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1174700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1617 bp

ATGAGCCGCTACGACAGCCGCTCCGGCGACTCCACTTCCTACCGTGACCGGAGAAGCGATTCCGGATTTGGTGGGGCGACCGGCTTCGGTGGCTCAGCACGGTCTTCGTCAAGCAGGAGGGATGATGATGGTGCTAAGTCACCAAGGAAATTTGATTTGGATGGATTGATACCTTTTGAGAAGAATTTCTATGTTGAGTCCTCTCAAGTGGAAAAAATGTCGGAGGTGGAGATGGAGGAATATAGAAAACGGAGGGAAATTACTGTTGAAGGCCGTGATGTTCCAAAGCCCATCAAGAGTTTCCGTGACACTGGGTTTCCAGAATACGTTTTGGAACAAGTTACAAGGGCTGGATTTGTGGAACCTACACCAATTCAAGCTCAAGGATGGCCAATGGCTTTGAAGGGTCGCGATCTCATTGGTATTGCTGAAACAGGATCAGGGAAGACACTAGCCTACCTGTTGCCTGCAATTCTCCATGTCAATGCCCAGCCAATTCTGTCTCCTGGAGATGGCCCGATAGTACTAGTTTTAGCTCCAACCCGTGAACTTGCTGTTCAAATCCAACAAGAGGCCACTAAATTTGGTTCATCATCAAAAATTAAAAGCACATGCATATATGGCGGGGTTCCAAAGGGTCCTCAAGTTCGTGATCTGCAGAAAGGAGTTGAGATTGTAATTGCTACACCCGGGAGGTTGATTGATATGATAGAGTCGCATCATACCAACTTGCGAAGGGTCACGTACCTTGTGCTGGATGAGGCCGATCGGATGCTAGACATGGGGTTTGAACCTCAGATAAGGAAAATCGTTTCTCAGATTCGCCCAGACCGTCAAACATTATACTGGAGTGCTACCTGGCCAAAGGAGGTTGAACAACTGGCTAGGCAGTTCCTTTACAATCCATACAAAGTGATAATAGGTTCTCCAAATTTGAAAGCTAACCATTCGATCCACCAAATTGTTGACATCATTACTGAAAATCAGAAATATAACAAATTGGTAAAGTTGCTAGAGGATATTATGGATGGCAGCAGAATACTGATATTCATGGATACTAAGAAAGGATGTGATCAGATCACTCGGCAGCTCCGCATGGATGGATGGCCTGCTCTTTCGATTCATGGTGACAAAAGTCAAGCAGAAAGAGATTGGGTCCTCTCCGAGTTTAAAGATGGCAAGAGCCCCATAATGACTGCAACTGATGTTGCTGCTCGTGGTCTAGGTATGGAAAGAATTTATTTTAAGCTTGTAAGGTTTTGGCGTGCATCGGATTTGCCTTTGGGATTTGGTAGGCAAAAACTGCTAACTTTGGGGTGCATAGATGTTAAGGATGTGAAATTTGTTATAAACTATGATTTTCCTGGATCCCTCGAAGATTATGTTCACCGCATTGGCCGAACTGGAAGAGCTGGGGCCAAAGGAACAGCATACACGTTCTTTACAGCTGCTAATGCTAGATTTGCAAAGGAACTTATTGCCTTGCTTGAGGAAGCTGGACAGAAGGTCAGTCCTGAGTTGGCAGCAATGGGTCGTGGTGCACCACCTCCCCCCTCAGGTCACGGGGTTTTTCGAGATCGTGGTAGGGGTTATGGAGGCGGTCGTCCTTGGAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03040 ko05202 ko05205 map03040 map05202 map05205

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

539

Amino Acids

60.04

Weight (kDa)

9.28

Isoelectric Point (pI)

40.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	123 - 293	2e-50	DEAD/DEAH box helicase
ResIII	PF04851	142 - 286	7.1e-07	Type III restriction enzyme, res subunit
Helicase_C	PF00271	330 - 410	4e-15	Helicase conserved C-terminal domain
Helicase_C	PF00271	441 - 472	7.1e-09	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004596)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55150 AT1G55150
fragaria_vesca	FvH4_6g33220 FvH4_6g33220 FvH4_6g33220 FvH4_6g33220 FvH4_6g33220 FvH4_6g33220 FvH4_6g33220
malus_domestica	MD09G1194200.v1.1 MD17G1174700.v1.1
prunus_persica	Prupe.3G019300_v2.0.a1 Prupe.3G019300_v2.0.a1 Prupe.3G050700_v2.0.a1 Prupe.3G050700_v2.0.a1 Prupe.3G050700_v2.0.a1
pyrus_communis	pycom09g11070
rosa_chinensis	RchiOBHm_Chr2g0142931
rosa_laevigata	RLG00000019997
rosa_multiflora	Rmu_sc0028648.1_g000001
rosa_roxburghii	Rroxscaffold_2G00102220 Rroxscaffold_2G00103260
rosa_rugosa	Rorug02G0377000 Rorug02G0377100 Rorug02G0377200
rosa_samantha	Rh2AG430000 Rh2BG439000 Rh2CG362000 Rh2CG415500 Rh2DG449000
rosa_wichuraiana	Rw2G035130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1349
AccBSI	CCGCTC	1 cut(s)	21
AccII	CGCG	1 cut(s)	411
AccIII	TCCGGA	1 cut(s)	62
AciI	CCGC	6 cut(s)	7, 19, 624, 1093, 1390, 1599
AclWI	GGATC	4 cut(s)	445, 946, 1362, 1375
AcoI	YGGCCR	3 cut(s)	389, 860, 1396
AcsI	RAATTY	6 cut(s)	149, 184, 581, 931, 1236, 1340
AfaI	GTAC	2 cut(s)	525, 746
AfiI	CCNNNNNNNGG	4 cut(s)	512, 571, 754, 1556
AflIII	ACRYGT	1 cut(s)	1434
AgsI	TTSAA	7 cut(s)	269, 377, 403, 560, 791, 875, 937
AhdI	GACNNNNNGTC	1 cut(s)	1505
AhlI	ACTAGT	1 cut(s)	526
AjnI	CCWGG	3 cut(s)	505, 857, 1363
AjuI	GAANNNNNNNTTGG	2 cut(s)	140, 172
AluBI	AGCT	8 cut(s)	380, 536, 941, 1090, 1249, 1412, 1448, 1496
AluI	AGCT	8 cut(s)	380, 536, 941, 1090, 1249, 1412, 1448, 1496
Alw21I	GWGCWC	1 cut(s)	1543
Alw26I	GTCTC	1 cut(s)	507
Alw44I	GTGCAC	1 cut(s)	1539
AlwI	GGATC	4 cut(s)	445, 946, 1362, 1375
Ama87I	CYCGRG	1 cut(s)	689
Aor13HI	TCCGGA	1 cut(s)	62
AoxI	GGCC	9 cut(s)	271, 389, 514, 573, 762, 860, 1106, 1396, 1416
ApaLI	GTGCAC	1 cut(s)	1539
ApeKI	GCWGC	5 cut(s)	1032, 1087, 1211, 1448, 1523
ApoI	RAATTY	6 cut(s)	149, 184, 581, 931, 1236, 1340
ArsI	GACNNNNNNTTYG	2 cut(s)	770, 802
Asp700I	GAANNNNTTC	2 cut(s)	58, 185
AspS9I	GGNCC	4 cut(s)	515, 638, 1156, 1416
AsuC2I	CCSGG	2 cut(s)	690, 691
AsuHPI	GGTGA	3 cut(s)	132, 1139, 1379
AvaI	CYCGRG	1 cut(s)	689
AvaII	GGWCC	2 cut(s)	638, 1156
AxyI	CCTNAGG	1 cut(s)	1555
BaeGI	GKGCMC	1 cut(s)	1543
BalI	TGGCCA	2 cut(s)	391, 862
BamHI	GGATCC	1 cut(s)	1367
BanII	GRGCYC	1 cut(s)	1187
BauI	CACGAG	1 cut(s)	1215
BbsI	GAAGAC	2 cut(s)	96, 452
Bbv12I	GWGCWC	1 cut(s)	1543
BbvI	GCAGC	5 cut(s)	1044, 1099, 1198, 1435, 1535
BceAI	ACGGC	1 cut(s)	258
BcgI	CGANNNNNNTGC	2 cut(s)	87, 121
BciT130I	CCWGG	3 cut(s)	507, 859, 1365
BciVI	GTATCC	1 cut(s)	1048
BclI	TGATCA	1 cut(s)	1072
BcnI	CCSGG	2 cut(s)	690, 691
BcoDI	GTCTC	1 cut(s)	507
BcuI	ACTAGT	1 cut(s)	526
BfaI	CTAG	7 cut(s)	452, 527, 776, 885, 1013, 1223, 1458
BfmI	CTRYAG	1 cut(s)	656
BfuI	GTATCC	1 cut(s)	1048
BglI	GCCNNNNNGGC	1 cut(s)	24
BisI	GCNGC	7 cut(s)	7, 19, 1033, 1088, 1212, 1449, 1524
BlpI	GCTNAGC	1 cut(s)	94
BlsI	GCNGC	7 cut(s)	8, 20, 1034, 1089, 1213, 1450, 1525
BmcAI	AGTACT	1 cut(s)	525
Bme1390I	CCNGG	5 cut(s)	507, 690, 691, 859, 1365
Bme18I	GGWCC	2 cut(s)	638, 1156
BmeRI	GACNNNNNGTC	1 cut(s)	1505
BmeT110I	CYCGRG	1 cut(s)	689
BmgT120I	GGNCC	4 cut(s)	515, 638, 1156, 1416
BmiI	GGNNCC	6 cut(s)	363, 630, 639, 1157, 1369, 1417
BmrFI	CCNGG	5 cut(s)	507, 690, 691, 859, 1365
BmrI	ACTGGG	1 cut(s)	321
BmsI	GCATC	3 cut(s)	727, 762, 1277
BmuI	ACTGGG	1 cut(s)	321
BoxI	GACNNNNGTC	1 cut(s)	1134
BpiI	GAAGAC	2 cut(s)	96, 452
BpmI	CTGGAG	2 cut(s)	528, 868
Bpu1102I	GCTNAGC	1 cut(s)	94
BpuEI	CTTGAG	4 cut(s)	189, 366, 627, 1508
BpuMI	CCSGG	2 cut(s)	690, 691
BsaAI	YACGTR	1 cut(s)	744
BsaJI	CCNNGG	3 cut(s)	143, 689, 1607
BsaWI	WCCGGW	2 cut(s)	48, 62
BsaXI	ACNNNNNCTCC	2 cut(s)	5, 35
Bsc4I	CCNNNNNNNGG	4 cut(s)	512, 571, 754, 1556
Bse118I	RCCGGY	1 cut(s)	80
Bse1I	ACTGG	4 cut(s)	316, 851, 885, 1408
Bse21I	CCTNAGG	1 cut(s)	1555
Bse3DI	GCAATG	1 cut(s)	1533
BseAI	TCCGGA	1 cut(s)	62
BseBI	CCWGG	3 cut(s)	507, 859, 1365
BseDI	CCNNGG	3 cut(s)	143, 689, 1607
BseLI	CCNNNNNNNGG	4 cut(s)	512, 571, 754, 1556
BseMI	GCAATG	1 cut(s)	1533
BseMII	CTCAG	5 cut(s)	108, 809, 830, 1506, 1569
BseNI	ACTGG	4 cut(s)	316, 851, 885, 1408
BseSI	GKGCMC	1 cut(s)	1543
BseXI	GCAGC	5 cut(s)	1044, 1099, 1198, 1435, 1535
BseYI	CCCAGC	2 cut(s)	489, 1412
Bsh1236I	CGCG	1 cut(s)	411
Bsh1285I	CGRYCG	3 cut(s)	81, 769, 1603
BshFI	GGCC	9 cut(s)	273, 391, 516, 575, 764, 862, 1108, 1398, 1418
BsiEI	CGRYCG	3 cut(s)	81, 769, 1603
BsiHKAI	GWGCWC	1 cut(s)	1543
BsiHKCI	CYCGRG	1 cut(s)	689
BsiSI	CCGG	5 cut(s)	24, 49, 63, 81, 690
BslI	CCNNNNNNNGG	4 cut(s)	512, 571, 754, 1556
BsmAI	GTCTC	1 cut(s)	507
BsnI	GGCC	9 cut(s)	273, 391, 516, 575, 764, 862, 1108, 1398, 1418
BsoBI	CYCGRG	1 cut(s)	689
Bsp1286I	GDGCHC	2 cut(s)	1187, 1543
Bsp13I	TCCGGA	1 cut(s)	62
Bsp143I	GATC	9 cut(s)	412, 437, 652, 766, 951, 1072, 1077, 1367, 1576
Bsp1720I	GCTNAGC	1 cut(s)	94
Bsp68I	TCGCGA	1 cut(s)	411
BspACI	CCGC	6 cut(s)	7, 19, 624, 1093, 1390, 1599
BspANI	GGCC	9 cut(s)	273, 391, 516, 575, 764, 862, 1108, 1398, 1418
BspCNI	CTCAG	5 cut(s)	107, 808, 829, 1507, 1568
BspEI	TCCGGA	1 cut(s)	62
BspFNI	CGCG	1 cut(s)	411
BspLI	GGNNCC	6 cut(s)	363, 630, 639, 1157, 1369, 1417
BspMAI	CTGCAG	1 cut(s)	660
BspPI	GGATC	4 cut(s)	445, 946, 1362, 1375
BspQI	GCTCTTC	1 cut(s)	1402
BsrBI	CCGCTC	1 cut(s)	21
BsrDI	GCAATG	1 cut(s)	1533
BsrFI	RCCGGY	1 cut(s)	80
BsrI	ACTGG	4 cut(s)	316, 851, 885, 1408
BssAI	RCCGGY	1 cut(s)	80
BssECI	CCNNGG	3 cut(s)	143, 689, 1607
BssMI	GATC	9 cut(s)	412, 437, 652, 766, 951, 1072, 1077, 1367, 1576
BssSI	CACGAG	1 cut(s)	1215
BssT1I	CCWWGG	2 cut(s)	143, 1607
Bst2BI	CACGAG	1 cut(s)	1215
Bst2UI	CCWGG	3 cut(s)	507, 859, 1365
Bst4CI	ACNGT	4 cut(s)	44, 102, 265, 833
Bst6I	CTCTTC	1 cut(s)	1402
BstAPI	GCANNNNNTGC	1 cut(s)	1303
BstBAI	YACGTR	1 cut(s)	744
BstC8I	GCNNGC	3 cut(s)	468, 1110, 1266
BstDEI	CTNAG	7 cut(s)	94, 135, 795, 816, 1059, 1515, 1555
BstFNI	CGCG	1 cut(s)	411
BstKTI	GATC	9 cut(s)	415, 440, 655, 769, 954, 1075, 1080, 1370, 1579
BstMAI	GTCTC	1 cut(s)	507
BstMBI	GATC	9 cut(s)	412, 437, 652, 766, 951, 1072, 1077, 1367, 1576
BstMCI	CGRYCG	3 cut(s)	81, 769, 1603
BstMWI	GCNNNNNNNGC	7 cut(s)	15, 24, 90, 621, 1303, 1454, 1463
BstNI	CCWGG	3 cut(s)	507, 859, 1365
BstNSI	RCATGY	1 cut(s)	615
BstPAI	GACNNNNGTC	1 cut(s)	1134
BstSCI	CCNGG	5 cut(s)	505, 688, 689, 857, 1363
BstSFI	CTRYAG	1 cut(s)	656
BstSLI	GKGCMC	1 cut(s)	1543
BstUI	CGCG	1 cut(s)	411
BstV1I	GCAGC	5 cut(s)	1044, 1099, 1198, 1435, 1535
BstV2I	GAAGAC	2 cut(s)	96, 452
BstX2I	RGATCY	1 cut(s)	1367
BstYI	RGATCY	1 cut(s)	1367
Bsu36I	CCTNAGG	1 cut(s)	1555
BsuI	GTATCC	1 cut(s)	1048
BsuRI	GGCC	9 cut(s)	273, 391, 516, 575, 764, 862, 1108, 1398, 1418
BtsIMutI	CAGTG	1 cut(s)	309
BtuMI	TCGCGA	1 cut(s)	411
Cac8I	GCNNGC	3 cut(s)	468, 1110, 1266
Cfr10I	RCCGGY	1 cut(s)	80
Cfr13I	GGNCC	4 cut(s)	515, 638, 1156, 1416
Cfr9I	CCCGGG	1 cut(s)	689
Csp6I	GTAC	2 cut(s)	524, 745
CspCI	CAANNNNNGTGG	2 cut(s)	565, 600
CviAII	CATG	6 cut(s)	479, 612, 781, 1051, 1096, 1124
CviQI	GTAC	2 cut(s)	524, 745
DdeI	CTNAG	7 cut(s)	94, 135, 795, 816, 1059, 1515, 1555
DpnI	GATC	9 cut(s)	414, 439, 654, 768, 953, 1074, 1079, 1369, 1578
DpnII	GATC	9 cut(s)	412, 437, 652, 766, 951, 1072, 1077, 1367, 1576
DraI	TTTAAA	1 cut(s)	1171
DriI	GACNNNNNGTC	1 cut(s)	1505
EaeI	YGGCCR	3 cut(s)	389, 860, 1396
Eam1104I	CTCTTC	1 cut(s)	1402
Eam1105I	GACNNNNNGTC	1 cut(s)	1505
EarI	CTCTTC	1 cut(s)	1402
Eco130I	CCWWGG	2 cut(s)	143, 1607
Eco24I	GRGCYC	1 cut(s)	1187
Eco47I	GGWCC	2 cut(s)	638, 1156
Eco81I	CCTNAGG	1 cut(s)	1555
Eco88I	CYCGRG	1 cut(s)	689
EcoO109I	RGGNCCY	2 cut(s)	638, 1156
EcoRII	CCWGG	3 cut(s)	505, 857, 1363
EcoT14I	CCWWGG	2 cut(s)	143, 1607
EcoT22I	ATGCAT	1 cut(s)	617
EcoT38I	GRGCYC	1 cut(s)	1187
ErhI	CCWWGG	2 cut(s)	143, 1607
FaeI	CATG	6 cut(s)	482, 615, 784, 1054, 1099, 1127
FatI	CATG	6 cut(s)	478, 611, 780, 1050, 1095, 1123
FauI	CCCGC	1 cut(s)	617
FbaI	TGATCA	1 cut(s)	1072
Fnu4HI	GCNGC	7 cut(s)	7, 19, 1033, 1088, 1212, 1449, 1524
FriOI	GRGCYC	1 cut(s)	1187
Fsp4HI	GCNGC	7 cut(s)	7, 19, 1033, 1088, 1212, 1449, 1524
FspBI	CTAG	7 cut(s)	452, 527, 776, 885, 1013, 1223, 1458
GluI	GCNGC	7 cut(s)	7, 19, 1033, 1088, 1212, 1449, 1524
GsaI	CCCAGC	2 cut(s)	493, 1416
GsuI	CTGGAG	2 cut(s)	528, 868
HaeIII	GGCC	9 cut(s)	273, 391, 516, 575, 764, 862, 1108, 1398, 1418
HapII	CCGG	5 cut(s)	24, 49, 63, 81, 690
Hin1II	CATG	6 cut(s)	482, 615, 784, 1054, 1099, 1127
HincII	GTYRAC	1 cut(s)	967
HindII	GTYRAC	1 cut(s)	967
HindIII	AAGCTT	1 cut(s)	1247
HinfI	GANTC	6 cut(s)	29, 59, 197, 713, 820, 1120
HpaII	CCGG	5 cut(s)	24, 49, 63, 81, 690
HphI	GGTGA	3 cut(s)	132, 1139, 1379
Hpy166II	GTNNAC	4 cut(s)	548, 967, 1387, 1541
Hpy188I	TCNGA	8 cut(s)	223, 771, 798, 819, 987, 1077, 1165, 1273
Hpy188III	TCNNGA	7 cut(s)	63, 295, 320, 410, 650, 1514, 1574
Hpy8I	GTNNAC	4 cut(s)	548, 967, 1387, 1541
HpyAV	CCTTC	4 cut(s)	263, 397, 729, 1498
HpyCH4III	ACNGT	4 cut(s)	44, 102, 265, 833
HpyCH4IV	ACGT	3 cut(s)	327, 743, 1436
HpyCH4V	TGCA	8 cut(s)	470, 615, 658, 1199, 1268, 1320, 1466, 1541
HpyF10VI	GCNNNNNNNGC	7 cut(s)	15, 24, 90, 621, 1303, 1454, 1463
HpyF3I	CTNAG	7 cut(s)	94, 135, 795, 816, 1059, 1515, 1555
HpySE526I	ACGT	3 cut(s)	327, 743, 1436
Hsp92II	CATG	6 cut(s)	482, 615, 784, 1054, 1099, 1127
Kpn2I	TCCGGA	1 cut(s)	62
Ksp22I	TGATCA	1 cut(s)	1072
Kzo9I	GATC	9 cut(s)	412, 437, 652, 766, 951, 1072, 1077, 1367, 1576
LguI	GCTCTTC	1 cut(s)	1402
LmnI	GCTCC	3 cut(s)	26, 541, 1095
Lsp1109I	GCAGC	5 cut(s)	1044, 1099, 1198, 1435, 1535
LweI	GCATC	3 cut(s)	727, 762, 1277
MaeI	CTAG	7 cut(s)	452, 527, 776, 885, 1013, 1223, 1458
MaeII	ACGT	3 cut(s)	327, 743, 1436
MaeIII	GTNAC	7 cut(s)	44, 138, 305, 340, 739, 1127, 1559
MalI	GATC	9 cut(s)	414, 439, 654, 768, 953, 1074, 1079, 1369, 1578
MbiI	CCGCTC	1 cut(s)	21
MboI	GATC	9 cut(s)	412, 437, 652, 766, 951, 1072, 1077, 1367, 1576
MboII	GAAGA	5 cut(s)	96, 193, 457, 1388, 1419
MflI	RGATCY	1 cut(s)	1367
MhlI	GDGCHC	2 cut(s)	1187, 1543
MlsI	TGGCCA	2 cut(s)	391, 862
MluNI	TGGCCA	2 cut(s)	391, 862
MlyI	GAGTC	3 cut(s)	23, 206, 722
MmeI	TCCRAC	3 cut(s)	201, 563, 589
Mox20I	TGGCCA	2 cut(s)	391, 862
Mph1103I	ATGCAT	1 cut(s)	617
MroI	TCCGGA	1 cut(s)	62
MroXI	GAANNNNTTC	2 cut(s)	58, 185
MscI	TGGCCA	2 cut(s)	391, 862
MseI	TTAA	4 cut(s)	603, 1170, 1245, 1329
MslI	CAYNNNNRTG	1 cut(s)	483
Msp20I	TGGCCA	2 cut(s)	391, 862
MspA1I	CMGCKG	1 cut(s)	1448
MspI	CCGG	5 cut(s)	24, 49, 63, 81, 690
MspR9I	CCNGG	5 cut(s)	507, 690, 691, 859, 1365
MvaI	CCWGG	3 cut(s)	507, 859, 1365
MvnI	CGCG	1 cut(s)	411
MwoI	GCNNNNNNNGC	7 cut(s)	15, 24, 90, 621, 1303, 1454, 1463
NciI	CCSGG	2 cut(s)	690, 691
NdeII	GATC	9 cut(s)	412, 437, 652, 766, 951, 1072, 1077, 1367, 1576
NlaIII	CATG	6 cut(s)	482, 615, 784, 1054, 1099, 1127
NlaIV	GGNNCC	6 cut(s)	363, 630, 639, 1157, 1369, 1417
NmeAIII	GCCGAG	1 cut(s)	1063
NmuCI	GTSAC	6 cut(s)	44, 138, 305, 739, 1127, 1559
NruI	TCGCGA	1 cut(s)	411
NsiI	ATGCAT	1 cut(s)	617
NspI	RCATGY	1 cut(s)	615
PciSI	GCTCTTC	1 cut(s)	1402
PcsI	WCGNNNNNNNCGW	1 cut(s)	829
PdmI	GAANNNNTTC	2 cut(s)	58, 185
PfeI	GAWTC	3 cut(s)	59, 820, 1120
PfoI	TCCNGGA	2 cut(s)	505, 1363
PkrI	GCNGC	7 cut(s)	8, 20, 1034, 1089, 1213, 1450, 1525
Ple19I	CGATCG	1 cut(s)	769
PleI	GAGTC	3 cut(s)	23, 205, 721
PpsI	GAGTC	3 cut(s)	23, 205, 721
Ppu21I	YACGTR	1 cut(s)	744
PpuMI	RGGWCCY	2 cut(s)	638, 1156
PshAI	GACNNNNGTC	1 cut(s)	1134
PsiI	TTATAA	1 cut(s)	1349
Psp5II	RGGWCCY	2 cut(s)	638, 1156
Psp6I	CCWGG	3 cut(s)	505, 857, 1363
PspFI	CCCAGC	2 cut(s)	489, 1412
PspGI	CCWGG	3 cut(s)	505, 857, 1363
PspN4I	GGNNCC	6 cut(s)	363, 630, 639, 1157, 1369, 1417
PspPI	GGNCC	4 cut(s)	515, 638, 1156, 1416
PspPPI	RGGWCCY	2 cut(s)	638, 1156
PstI	CTGCAG	1 cut(s)	660
PsuI	RGATCY	1 cut(s)	1367
PvuI	CGATCG	1 cut(s)	769
PvuII	CAGCTG	1 cut(s)	1448
RruI	TCGCGA	1 cut(s)	411
RsaI	GTAC	2 cut(s)	525, 746
RsaNI	GTAC	2 cut(s)	524, 745
RseI	CAYNNNNRTG	1 cut(s)	483
SapI	GCTCTTC	1 cut(s)	1402
SaqAI	TTAA	4 cut(s)	603, 1170, 1245, 1329
SatI	GCNGC	7 cut(s)	7, 19, 1033, 1088, 1212, 1449, 1524
Sau3AI	GATC	9 cut(s)	412, 437, 652, 766, 951, 1072, 1077, 1367, 1576
Sau96I	GGNCC	4 cut(s)	515, 638, 1156, 1416
ScaI	AGTACT	1 cut(s)	525
SchI	GAGTC	3 cut(s)	23, 206, 722
ScrFI	CCNGG	5 cut(s)	507, 690, 691, 859, 1365
SduI	GDGCHC	2 cut(s)	1187, 1543
SfaNI	GCATC	3 cut(s)	727, 762, 1277
SfcI	CTRYAG	1 cut(s)	656
SinI	GGWCC	2 cut(s)	638, 1156
SmaI	CCCGGG	1 cut(s)	691
SmiMI	CAYNNNNRTG	1 cut(s)	483
SmlI	CTYRAG	4 cut(s)	204, 381, 642, 1487
SmoI	CTYRAG	4 cut(s)	204, 381, 642, 1487
SpeI	ACTAGT	1 cut(s)	526
SsiI	CCGC	6 cut(s)	7, 19, 624, 1093, 1390, 1599
SspMI	CTAG	7 cut(s)	452, 527, 776, 885, 1013, 1223, 1458
StyD4I	CCNGG	5 cut(s)	505, 688, 689, 857, 1363
StyI	CCWWGG	2 cut(s)	143, 1607
TaaI	ACNGT	4 cut(s)	44, 102, 265, 833
TaiI	ACGT	3 cut(s)	330, 746, 1439
TaqI	TCGA	4 cut(s)	950, 1118, 1374, 1573
TatI	WGTACW	1 cut(s)	523
TauI	GCSGC	2 cut(s)	9, 21
TfiI	GAWTC	3 cut(s)	59, 820, 1120
Tru1I	TTAA	4 cut(s)	603, 1170, 1245, 1329
Tru9I	TTAA	4 cut(s)	603, 1170, 1245, 1329
TscAI	CASTG	1 cut(s)	316
TseFI	GTSAC	6 cut(s)	44, 138, 305, 739, 1127, 1559
TseI	GCWGC	5 cut(s)	1032, 1087, 1211, 1448, 1523
Tsp45I	GTSAC	6 cut(s)	44, 138, 305, 739, 1127, 1559
TspDTI	ATGAA	3 cut(s)	579, 1039, 1112
TspGWI	ACGGA	2 cut(s)	265, 293
TspMI	CCCGGG	1 cut(s)	689
TspRI	CASTG	1 cut(s)	316
VneI	GTGCAC	1 cut(s)	1539
VpaK11BI	GGWCC	2 cut(s)	638, 1156
XapI	RAATTY	6 cut(s)	149, 184, 581, 931, 1236, 1340
XceI	RCATGY	1 cut(s)	615
XmaI	CCCGGG	1 cut(s)	689
XmnI	GAANNNNTTC	2 cut(s)	58, 185
XspI	CTAG	7 cut(s)	452, 527, 776, 885, 1013, 1223, 1458
ZrmI	AGTACT	1 cut(s)	525
Zsp2I	ATGCAT	1 cut(s)	617

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.