Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0004717

Physical Location & Seq

Genomic Coordinates Reverse (-)

17785028 .. 17796018

Gene Structure 10991 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1195900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1431 bp

ATGCTTAACGTGAGTGTAAAATGGCAAAAGGAGCTGTTGAAAGCTGTGGAAATTGATACTACTCAGCCACCTTATGTATTTAAATGCCAACTATACGACTTAACAGGGGTACCTCCTGAAAGACAGAAGATAATGGTTAAAGGTGGTTTATTGAAGGATGATGCAGACTGGTCGACTCTAGGAGTAAAAGATGGTCAAAAATTGATGATGATGGGGACAGCAGATGAGGTTGTCAAGGCTCCAGAGAAGGCTATTGTATTTGCAGAAGATCTCCCAGAAGAAGAACAAGCTCTTCACTTGGGTTATGGTGCTGGTCTATTTAACTTGGGAAACACCTGCTATATGAACTCCACCATACAATGTCTGCATTCTGTTCCAGAGTTGAAATCAGCTTTGATCAAGTATTCACATTCTGAAAGAAGCAATGATGTGGATCAGACCTCTCATATGTTGACTATTGCAACTCGTGGTTTATTTAGTGAACTGGATAAAAGTGTCAAGGCTGTGGCACCCATGCAATTTTGGATGGTATTGCGCAAAAAATATCCTCAATTTGGTCAGCAGCATAATGGTACCTTCATGCAACAGGATGCTGAAGAATGTTGGTCACAGCTTTTATACACCCTTTCTCAATCTCTAAGATCGTCCGGTTCTAGTGAAAGTCCAGACTCTGTTAAAGCACTTTTCGGAATTGAACTTGTTAGCAGGGTGCACTGCGTAGAAAGTGGGGAAGAGAGCTCAGAAGCAGAGTCAGTTTATTCTCTGAAGTGCCACATATCACATGAGGTGAACCATTTGCATGAAGGGTTAAAACATGGTTTAAAATCAGAACTGGAAAAAAATTCTCCTTCCTTGGGGCGTAGCGCCATCTATGTCAAGGAGTCTCTTATCAATGGCCTTCCAAGGTACCTGACCATTCAGTTTGTACGTTTTTTCTGGAAGAGGGAATCAAATCAGAAGGCCAAGATTTTGCGGAAAGTCGATTACCCTTTGCAATTGGATATTTTTGATCTGTGTTCAGATGATCTTCGCAAAACATTGGAAGCTCCTCGTCAGAGACTGAGGGATGAGGAAGGTAAAAAACTTGGTTTGAAACCCAGTGAAAAGAGCTCTGATTCAAAAGACAAGGATATCGAGATGTCTGATGTGGAGGGATCCTCAAACAGAAGGGGAGAATCATCTAATCCTACATCAGATGAAGGTGCTATGACTGGAATTTATGATTTGGTTGCTGTGCTGACACACAAGGGTCGTAGTGCAGACTCTGGCCATTATGTTGCCTGGGTCAAGCAAGAAAGTGGGAAATGGATCCAATATGACGATGACAATCCCTACCCACAGAAGGAGGAAGACATCACCAAACTCTCTGGAGGAGGTGATTGGCATATGGCCTATATCTGCTTGTACAAGGCCCGTACCATCCGCATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005911 GO:0006464 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0016579 GO:0019538 GO:0030054 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0055044 GO:0070646 GO:0070647 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UCH ubiquitin

Protein Analysis

477

Amino Acids

53.73

Weight (kDa)

5.92

Isoelectric Point (pI)

48.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ubiquitin	PF00240	13 - 73	2.1e-08	Ubiquitin family
UCH_1	PF13423	104 - 441	3.7e-11	Ubiquitin carboxyl-terminal hydrolase
UCH	PF00443	105 - 469	1.1e-57	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004717)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51710 AT1G51710 AT3G21280 AT3G21280
fragaria_vesca	FvH4_6g32980 FvH4_6g32980 FvH4_6g32980 FvH4_6g32980 FvH4_6g32980
malus_domestica	MD09G1195900.v1.1 MD17G1176500.v1.1
prunus_persica	Prupe.3G052400_v2.0.a1 Prupe.3G052400_v2.0.a1
pyrus_communis	pycom09g11300 pycom12494g00050 pycom17g18440
rosa_chinensis	RchiOBHm_Chr2g0142531
rosa_laevigata	RLG00000019963
rosa_multiflora	Rmu_sc0007525.1_g000013 Rmu_sc0013104.1_g000001 Rmu_sc0040796.1_g000001
rosa_roxburghii	Rroxscaffold_2G00102550
rosa_rugosa	Rorug02G0373600 Rorug02G0373700 Rorug02G0373800 Rorug02G0373800
rosa_samantha	Rh2AG425000 Rh2BG434900 Rh2CG411600 Rh2DG444900
rosa_wichuraiana	Rw2G034860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	344
Acc16I	TGCGCA	1 cut(s)	536
Acc36I	ACCTGC	1 cut(s)	344
Acc65I	GGTACC	3 cut(s)	109, 572, 906
AccB1I	GGYRCC	4 cut(s)	109, 508, 572, 906
AccI	GTMKAC	1 cut(s)	173
AciI	CCGC	2 cut(s)	973, 1423
AclWI	GGATC	5 cut(s)	441, 1149, 1162, 1303, 1316
AcoI	YGGCCR	1 cut(s)	1267
AcsI	RAATTY	2 cut(s)	841, 1215
AcuI	CTGAAG	2 cut(s)	615, 785
AfaI	GTAC	6 cut(s)	111, 574, 908, 927, 1406, 1417
AfiI	CCNNNNNNNGG	3 cut(s)	554, 854, 1342
AgsI	TTSAA	6 cut(s)	40, 154, 385, 695, 1093, 1119
AjnI	CCWGG	1 cut(s)	1280
AluBI	AGCT	8 cut(s)	34, 44, 290, 392, 613, 738, 1046, 1110
AluI	AGCT	8 cut(s)	34, 44, 290, 392, 613, 738, 1046, 1110
Alw21I	GWGCWC	3 cut(s)	714, 740, 1112
Alw26I	GTCTC	2 cut(s)	888, 1051
Alw44I	GTGCAC	1 cut(s)	710
AlwI	GGATC	5 cut(s)	441, 1149, 1162, 1303, 1316
AlwNI	CAGNNNCTG	3 cut(s)	671, 1060, 1265
AoxI	GGCC	5 cut(s)	895, 960, 1267, 1389, 1410
ApaLI	GTGCAC	1 cut(s)	710
ApeKI	GCWGC	1 cut(s)	562
ApoI	RAATTY	2 cut(s)	841, 1215
Asp718I	GGTACC	3 cut(s)	109, 572, 906
AspLEI	GCGC	2 cut(s)	537, 866
AspS9I	GGNCC	1 cut(s)	1411
AsuHPI	GGTGA	3 cut(s)	799, 1348, 1388
BaeGI	GKGCMC	1 cut(s)	714
BaeI	ACNNNNGTAYC	2 cut(s)	93, 126
BalI	TGGCCA	1 cut(s)	1269
BamHI	GGATCC	2 cut(s)	1154, 1308
BanI	GGYRCC	4 cut(s)	109, 508, 572, 906
BanII	GRGCYC	2 cut(s)	740, 1112
BauI	CACGAG	1 cut(s)	465
BbsI	GAAGAC	1 cut(s)	1356
Bbv12I	GWGCWC	3 cut(s)	714, 740, 1112
BbvI	GCAGC	1 cut(s)	574
BccI	CCATC	5 cut(s)	185, 205, 520, 875, 1427
BcgI	CGANNNNNNTGC	2 cut(s)	153, 187
BciT130I	CCWGG	1 cut(s)	1282
BclI	TGATCA	1 cut(s)	396
BcoDI	GTCTC	2 cut(s)	888, 1051
BfaI	CTAG	2 cut(s)	179, 654
BfoI	RGCGCY	1 cut(s)	867
BfuAI	ACCTGC	1 cut(s)	344
BglII	AGATCT	1 cut(s)	268
BisI	GCNGC	1 cut(s)	563
BlsI	GCNGC	1 cut(s)	564
Bme1390I	CCNGG	1 cut(s)	1282
BmgT120I	GGNCC	1 cut(s)	1411
BmiI	GGNNCC	7 cut(s)	111, 240, 510, 574, 908, 1156, 1310
BmrFI	CCNGG	1 cut(s)	1282
BmrI	ACTGGG	1 cut(s)	1092
BmsI	GCATC	2 cut(s)	151, 580
BmuI	ACTGGG	1 cut(s)	1092
BpiI	GAAGAC	1 cut(s)	1356
BplI	GAGNNNNNCTC	2 cut(s)	1142, 1174
BpmI	CTGGAG	2 cut(s)	225, 1389
BsaBI	GATNNNNATC	2 cut(s)	432, 1326
BsaJI	CCNNGG	3 cut(s)	852, 902, 1281
BsaWI	WCCGGW	1 cut(s)	647
Bsc4I	CCNNNNNNNGG	3 cut(s)	554, 854, 1342
Bse1I	ACTGG	5 cut(s)	173, 489, 837, 1098, 1216
Bse3DI	GCAATG	1 cut(s)	430
Bse8I	GATNNNNATC	2 cut(s)	432, 1326
BseBI	CCWGG	1 cut(s)	1282
BseDI	CCNNGG	3 cut(s)	852, 902, 1281
BseGI	GGATG	5 cut(s)	163, 531, 595, 1072, 1419
BseJI	GATNNNNATC	2 cut(s)	432, 1326
BseLI	CCNNNNNNNGG	3 cut(s)	554, 854, 1342
BseMI	GCAATG	1 cut(s)	430
BseMII	CTCAG	3 cut(s)	77, 753, 1052
BseNI	ACTGG	5 cut(s)	173, 489, 837, 1098, 1216
BseRI	GAGGAG	2 cut(s)	1038, 1386
BseSI	GKGCMC	1 cut(s)	714
BseXI	GCAGC	1 cut(s)	574
BsgI	GTGCAG	1 cut(s)	1278
BshFI	GGCC	5 cut(s)	897, 962, 1269, 1391, 1412
BshNI	GGYRCC	4 cut(s)	109, 508, 572, 906
BsiHKAI	GWGCWC	3 cut(s)	714, 740, 1112
BsiSI	CCGG	1 cut(s)	648
BslFI	GGGAC	1 cut(s)	229
BslI	CCNNNNNNNGG	3 cut(s)	554, 854, 1342
BsmAI	GTCTC	2 cut(s)	888, 1051
BsmFI	GGGAC	1 cut(s)	229
BsmI	GAATGC	1 cut(s)	367
BsnI	GGCC	5 cut(s)	897, 962, 1269, 1391, 1412
Bsp1286I	GDGCHC	3 cut(s)	714, 740, 1112
Bsp1407I	TGTACA	1 cut(s)	1404
Bsp143I	GATC	8 cut(s)	268, 396, 433, 641, 1009, 1024, 1154, 1308
BspACI	CCGC	2 cut(s)	973, 1423
BspANI	GGCC	5 cut(s)	897, 962, 1269, 1391, 1412
BspCNI	CTCAG	3 cut(s)	76, 752, 1053
BspLI	GGNNCC	7 cut(s)	111, 240, 510, 574, 908, 1156, 1310
BspMI	ACCTGC	1 cut(s)	344
BspPI	GGATC	5 cut(s)	441, 1149, 1162, 1303, 1316
BspQI	GCTCTTC	1 cut(s)	297
BspT107I	GGYRCC	4 cut(s)	109, 508, 572, 906
BsrDI	GCAATG	1 cut(s)	430
BsrGI	TGTACA	1 cut(s)	1404
BsrI	ACTGG	5 cut(s)	173, 489, 837, 1098, 1216
BssECI	CCNNGG	3 cut(s)	852, 902, 1281
BssMI	GATC	8 cut(s)	268, 396, 433, 641, 1009, 1024, 1154, 1308
BssSI	CACGAG	1 cut(s)	465
BssT1I	CCWWGG	2 cut(s)	852, 902
Bst2BI	CACGAG	1 cut(s)	465
Bst2UI	CCWGG	1 cut(s)	1282
Bst6I	CTCTTC	3 cut(s)	297, 726, 935
BstAUI	TGTACA	1 cut(s)	1404
BstDEI	CTNAG	4 cut(s)	63, 638, 739, 1061
BstF5I	GGATG	5 cut(s)	163, 531, 595, 1072, 1419
BstH2I	RGCGCY	1 cut(s)	867
BstHHI	GCGC	2 cut(s)	537, 866
BstKTI	GATC	8 cut(s)	271, 399, 436, 644, 1012, 1027, 1157, 1311
BstMAI	GTCTC	2 cut(s)	888, 1051
BstMBI	GATC	8 cut(s)	268, 396, 433, 641, 1009, 1024, 1154, 1308
BstMWI	GCNNNNNNNGC	1 cut(s)	31
BstNI	CCWGG	1 cut(s)	1282
BstNSI	RCATGY	1 cut(s)	1429
BstSCI	CCNGG	1 cut(s)	1280
BstSLI	GKGCMC	1 cut(s)	714
BstV1I	GCAGC	1 cut(s)	574
BstV2I	GAAGAC	1 cut(s)	1356
BstX2I	RGATCY	3 cut(s)	268, 1154, 1308
BstYI	RGATCY	3 cut(s)	268, 1154, 1308
BsuRI	GGCC	5 cut(s)	897, 962, 1269, 1391, 1412
BtsCI	GGATG	5 cut(s)	163, 531, 595, 1072, 1419
BtsI	GCAGTG	1 cut(s)	712
BtsIMutI	CAGTG	2 cut(s)	712, 1105
BveI	ACCTGC	1 cut(s)	344
CaiI	CAGNNNCTG	3 cut(s)	671, 1060, 1265
CfoI	GCGC	2 cut(s)	537, 866
Cfr13I	GGNCC	1 cut(s)	1411
Csp6I	GTAC	6 cut(s)	110, 573, 907, 926, 1405, 1416
CviAII	CATG	6 cut(s)	514, 580, 782, 800, 815, 1426
CviQI	GTAC	6 cut(s)	110, 573, 907, 926, 1405, 1416
DdeI	CTNAG	4 cut(s)	63, 638, 739, 1061
DpnI	GATC	8 cut(s)	270, 398, 435, 643, 1011, 1026, 1156, 1310
DpnII	GATC	8 cut(s)	268, 396, 433, 641, 1009, 1024, 1154, 1308
DraI	TTTAAA	2 cut(s)	82, 822
EaeI	YGGCCR	1 cut(s)	1267
Eam1104I	CTCTTC	3 cut(s)	297, 726, 935
EarI	CTCTTC	3 cut(s)	297, 726, 935
Ecl136II	GAGCTC	2 cut(s)	738, 1110
Eco130I	CCWWGG	2 cut(s)	852, 902
Eco24I	GRGCYC	2 cut(s)	740, 1112
Eco32I	GATATC	1 cut(s)	1132
Eco53kI	GAGCTC	2 cut(s)	738, 1110
Eco57I	CTGAAG	2 cut(s)	615, 785
EcoICRI	GAGCTC	2 cut(s)	738, 1110
EcoRII	CCWGG	1 cut(s)	1280
EcoRV	GATATC	1 cut(s)	1132
EcoT14I	CCWWGG	2 cut(s)	852, 902
EcoT38I	GRGCYC	2 cut(s)	740, 1112
ErhI	CCWWGG	2 cut(s)	852, 902
FaeI	CATG	6 cut(s)	517, 583, 785, 803, 818, 1429
FaqI	GGGAC	1 cut(s)	229
FatI	CATG	6 cut(s)	513, 579, 781, 799, 814, 1425
FauNDI	CATATG	2 cut(s)	447, 1386
FbaI	TGATCA	1 cut(s)	396
FblI	GTMKAC	1 cut(s)	173
Fnu4HI	GCNGC	1 cut(s)	563
FokI	GGATG	5 cut(s)	170, 538, 602, 1079, 1406
FriOI	GRGCYC	2 cut(s)	740, 1112
Fsp4HI	GCNGC	1 cut(s)	563
FspBI	CTAG	2 cut(s)	179, 654
FspI	TGCGCA	1 cut(s)	536
GlaI	GCGC	2 cut(s)	536, 865
GluI	GCNGC	1 cut(s)	563
GsuI	CTGGAG	2 cut(s)	225, 1389
HaeII	RGCGCY	1 cut(s)	867
HaeIII	GGCC	5 cut(s)	897, 962, 1269, 1391, 1412
HapII	CCGG	1 cut(s)	648
HhaI	GCGC	2 cut(s)	537, 866
Hin1II	CATG	6 cut(s)	517, 583, 785, 803, 818, 1429
Hin6I	GCGC	2 cut(s)	535, 864
HinP1I	GCGC	2 cut(s)	535, 864
HincII	GTYRAC	2 cut(s)	174, 453
HindII	GTYRAC	2 cut(s)	174, 453
HinfI	GANTC	8 cut(s)	175, 668, 749, 881, 947, 1115, 1175, 1262
HpaII	CCGG	1 cut(s)	648
HphI	GGTGA	3 cut(s)	799, 1348, 1388
Hpy166II	GTNNAC	5 cut(s)	174, 453, 482, 712, 790
Hpy188III	TCNNGA	7 cut(s)	116, 242, 377, 665, 937, 1135, 1368
Hpy8I	GTNNAC	5 cut(s)	174, 453, 482, 712, 790
HpyCH4IV	ACGT	2 cut(s)	9, 928
HpyF10VI	GCNNNNNNNGC	1 cut(s)	31
HpyF3I	CTNAG	4 cut(s)	63, 638, 739, 1061
HpySE526I	ACGT	2 cut(s)	9, 928
Hsp92II	CATG	6 cut(s)	517, 583, 785, 803, 818, 1429
HspAI	GCGC	2 cut(s)	535, 864
KpnI	GGTACC	3 cut(s)	113, 576, 910
Ksp22I	TGATCA	1 cut(s)	396
Kzo9I	GATC	8 cut(s)	268, 396, 433, 641, 1009, 1024, 1154, 1308
LguI	GCTCTTC	1 cut(s)	297
LmnI	GCTCC	3 cut(s)	31, 244, 1051
Lsp1109I	GCAGC	1 cut(s)	574
LweI	GCATC	2 cut(s)	151, 580
MaeI	CTAG	2 cut(s)	179, 654
MaeII	ACGT	2 cut(s)	9, 928
MaeIII	GTNAC	1 cut(s)	606
MalI	GATC	8 cut(s)	270, 398, 435, 643, 1011, 1026, 1156, 1310
MboI	GATC	8 cut(s)	268, 396, 433, 641, 1009, 1024, 1154, 1308
MfeI	CAATTG	1 cut(s)	995
MflI	RGATCY	3 cut(s)	268, 1154, 1308
MhlI	GDGCHC	3 cut(s)	714, 740, 1112
MlsI	TGGCCA	1 cut(s)	1269
MluCI	AATT	8 cut(s)	51, 200, 518, 551, 690, 841, 995, 1215
MluNI	TGGCCA	1 cut(s)	1269
MlyI	GAGTC	5 cut(s)	169, 662, 758, 890, 1256
Mox20I	TGGCCA	1 cut(s)	1269
MscI	TGGCCA	1 cut(s)	1269
MseI	TTAA	8 cut(s)	6, 81, 101, 138, 321, 675, 809, 821
MslI	CAYNNNNRTG	2 cut(s)	798, 1424
Msp20I	TGGCCA	1 cut(s)	1269
MspI	CCGG	1 cut(s)	648
MspR9I	CCNGG	1 cut(s)	1282
MunI	CAATTG	1 cut(s)	995
Mva1269I	GAATGC	1 cut(s)	367
MvaI	CCWGG	1 cut(s)	1282
MwoI	GCNNNNNNNGC	1 cut(s)	31
NdeI	CATATG	2 cut(s)	447, 1386
NdeII	GATC	8 cut(s)	268, 396, 433, 641, 1009, 1024, 1154, 1308
NlaIII	CATG	6 cut(s)	517, 583, 785, 803, 818, 1429
NlaIV	GGNNCC	7 cut(s)	111, 240, 510, 574, 908, 1156, 1310
NmuCI	GTSAC	1 cut(s)	606
NsbI	TGCGCA	1 cut(s)	536
NspI	RCATGY	1 cut(s)	1429
PaqCI	CACCTGC	1 cut(s)	344
PciSI	GCTCTTC	1 cut(s)	297
PctI	GAATGC	1 cut(s)	367
PfeI	GAWTC	3 cut(s)	947, 1115, 1175
PkrI	GCNGC	1 cut(s)	564
PleI	GAGTC	5 cut(s)	169, 662, 757, 889, 1256
PpsI	GAGTC	5 cut(s)	169, 662, 757, 889, 1256
Psp124BI	GAGCTC	2 cut(s)	740, 1112
Psp6I	CCWGG	1 cut(s)	1280
PspGI	CCWGG	1 cut(s)	1280
PspN4I	GGNNCC	7 cut(s)	111, 240, 510, 574, 908, 1156, 1310
PspPI	GGNCC	1 cut(s)	1411
PstNI	CAGNNNCTG	3 cut(s)	671, 1060, 1265
PsuI	RGATCY	3 cut(s)	268, 1154, 1308
RsaI	GTAC	6 cut(s)	111, 574, 908, 927, 1406, 1417
RsaNI	GTAC	6 cut(s)	110, 573, 907, 926, 1405, 1416
RseI	CAYNNNNRTG	2 cut(s)	798, 1424
SacI	GAGCTC	2 cut(s)	740, 1112
SalI	GTCGAC	1 cut(s)	172
SapI	GCTCTTC	1 cut(s)	297
SaqAI	TTAA	8 cut(s)	6, 81, 101, 138, 321, 675, 809, 821
SatI	GCNGC	1 cut(s)	563
Sau3AI	GATC	8 cut(s)	268, 396, 433, 641, 1009, 1024, 1154, 1308
Sau96I	GGNCC	1 cut(s)	1411
SchI	GAGTC	5 cut(s)	169, 662, 758, 890, 1256
ScrFI	CCNGG	1 cut(s)	1282
SduI	GDGCHC	3 cut(s)	714, 740, 1112
SfaNI	GCATC	2 cut(s)	151, 580
SmiI	ATTTAAAT	1 cut(s)	82
SmiMI	CAYNNNNRTG	2 cut(s)	798, 1424
Sse9I	AATT	8 cut(s)	51, 200, 518, 551, 690, 841, 995, 1215
SsiI	CCGC	2 cut(s)	973, 1423
SspMI	CTAG	2 cut(s)	179, 654
SstI	GAGCTC	2 cut(s)	740, 1112
StyD4I	CCNGG	1 cut(s)	1280
StyI	CCWWGG	2 cut(s)	852, 902
SwaI	ATTTAAAT	1 cut(s)	82
TaiI	ACGT	2 cut(s)	12, 931
TaqI	TCGA	3 cut(s)	173, 981, 1134
TasI	AATT	8 cut(s)	51, 200, 518, 551, 690, 841, 995, 1215
TatI	WGTACW	1 cut(s)	1404
TfiI	GAWTC	3 cut(s)	947, 1115, 1175
Tru1I	TTAA	8 cut(s)	6, 81, 101, 138, 321, 675, 809, 821
Tru9I	TTAA	8 cut(s)	6, 81, 101, 138, 321, 675, 809, 821
TscAI	CASTG	2 cut(s)	719, 1105
TseFI	GTSAC	1 cut(s)	606
TseI	GCWGC	1 cut(s)	562
Tsp45I	GTSAC	1 cut(s)	606
TspDTI	ATGAA	4 cut(s)	359, 568, 816, 1212
TspRI	CASTG	2 cut(s)	719, 1105
VneI	GTGCAC	1 cut(s)	710
XapI	RAATTY	2 cut(s)	841, 1215
XceI	RCATGY	1 cut(s)	1429
XmiI	GTMKAC	1 cut(s)	173
XspI	CTAG	2 cut(s)	179, 654

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.