Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0005377

Physical Location & Seq

Genomic Coordinates Forward (+)

20563729 .. 20569478

Gene Structure 5750 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1210800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3078 bp

ATGAAAAGGGAAGAGGAAACAGAAGAGGAGGCGGAGTCCCCAAAATTCCCAGCATTTCCGTACAAACCCTACGACATACAGATCGATTTCATGAAGGCCCTCTATCACTCTCTCAACAAAGGAGGCCTTTCCATGCTCGAAAGCCCCACCGGCAAGTCATCACTCTTCTGCGGATACTTCTGCTTTCATTTCTCTGATTTTTATGCTTCTCCGTTTCGAAAATTGAACCGTTCGAGGACTGGGAAAACTCTAAGTATCATTTGTAGTGCTCTGCAATGGGTGGTTGATAGGAGGGAACAACAGAAATCTGAAAATTGTGTTGAATCTGATCTTAGTAGTGCTAAAGATGGTCGAGTTGACTCGGATGATGAGCCTGACTGGATGAGAAATGTTGTTATTAACAAAGACAAGCAGAGTGAGGAGAAGAAGTCTAATAAGAAGGAGAAATTTGGGGTTGGGTTTCGAAGGGCCAATAAGAGAGGGAGCCGTGGAAATTGTAGAGATTTGTTCTCGCAGAGTGTTGAGGAGGAGCCTTCCGCAAAGAAAGAGGAAAAGGATTTGCTAATGAGCAATGATGCTGTGGAGTTAAGTGATGAGGAGTTTTTGGTGGAAGATTATGAAAGCGAAGAGGAAGGGGATCTGGGTGGTGGGAAGTCAAAGAGGAAGCTCTCTGGGGTTTCTTTTAGTTCGTCAAGCGAGGAAGATGAAGAAGATGGGTCTGGTGACGATGAATTTGAGGATGAGAAGGTGCAGGTTTATTTTTGTAGCCGGACACATTCACAGCTTTCACAGTTCATAAAGGAATTAAGGAAGACGATCTTTGCTAATATGATGAAGGTGGTGTCTTTGGGCTCAAGGAAGAATTGCTGCATCAATGAAGGTATGAAACTCATCACTGAATTAGTAGATTATAGTTTGTGTATTGTTACATTCTTCTGGGGAATGGTTTCAGAGGTATTGAAACTTGGAAACTCAACTCGCATCAATGAACGCTGTTTGGAACTTCAAAAGAACAAGAATAAGGATGTTTCCAAAATCAAGAACTTAGGTGCCGGAGTCAGGATTCGCAGGAACAATGCTTCTTGTGGATGCCCGATGCTTAGAAAACATAAACTACAAAAAGAATTCAGGAGCATGATGTCTCAACATGGTGCCTTGGATATTGAAGATCTTGTTGATCTTGGAAAAAGCATGAGGACTTGTCCATATTATGGCTCCCGAAGCATGGTTCCTGTTGCTGATCTGGTGGTTCTACCGTATCAGTCTCTTCTTTCTAAAGCATCTCGTGAGTCTCTTGGTCTCAAGTTAAAGAATAATATTGTTATTATAGACGAGGCTCACAATCTAGCTGACTCCTTGATCAGCATGTATGACTCAAGAATCACTTTTTCGCAGCTGGAGGATGTGCACGGGCACATTGAGAAGTACTTTGCAAGATTTTGCAATGTATTGGGACCTGGTAATCGTAGATATATTCAAACTCTCATGGTGCTCACTCGGGCTTTCCTCCAAGTGTTTCTAAGAGAGGATACGGGCTGCGGAAATTCTTGTCATGATACTGAAAAGGCTTCTGGAGCAAATGGAACGCCTACTTCCTCTATGGCCATTAATGACTTTTTATTTTCCCTCAATATAGACAACATCAACTTGGTCAAATTGCTTCAGTATATAAAGGAAAGCAACATCATGCGCAAGGTCTGTGGTTATGGCGATAAAGTAGGCATTCAAAAGAGTTTGGCACTTAATGGCGGTGAAGAGAGAAGCACTTTGTCCAGTTTCCAAGCATTAGCAAATTTTTTGCTATCACTGACAAATAAAGATGGAGATGGGAGGATTATAATCTCAAAGAGTAGTCCAACATGCCCTGGACAGGAAGGAGGATATATAAAATATGTTATGCTGACTGGGGATAATATTTTTTCAGAGATTGTGGATCAAGCGCATGCTGTTGTACTGGCTGGGGGAACTCTCCAACCTATAGAAGAAACAAGGGAGAGACTCTTCCCTTGGTTACCACTGAACCAGTTGCAATTCTTTTCATGCAGCCACATTGTTCCCCCCGAGAGCATTCTGCCAGTTGCAGTTTCCCGTGGTCCTTCTGGTCACACTTTTGACTTCAGCTACAGCTCCAGGAGGTCATCAATCATGGCAACTACTGTGTCAATATTTTTTCTCCGATTTTGCTTTAAAACTTTTAAAACGCAGTTCATTCCCAGGGGAAAAATCACTTCTGCTGTCGATTACAGTAGCACCTTTTTCTGCTTATTACTTCTTTTTTGGGATTCGTACTGGATACAGGAGCTAGGGCGTTTGGTTTGCAATTTGGTGACCGCAGTTCCTGAGGGCATCGTTGTCTTCTTCTCTTCATTTGACTATGAAGGACAAGTTTATGATGCCTGGGAAGCTTCAGGTATCCTTGAAAGGATTAAGAAGAAGAAGCGTCTATTTAGAGAACCTAGGAAGAGTACACATGTAGAATCTGTTCTCAAGGAATATAAGGAAACAATTGAAACATTGTCTTCTGGAGAGGGGAAAGAAAATCCTTCTCAGAGTGGTGCAATACTTCTTGCTGTCGTCGGTGGTAAGATATCAGAAGGCATCAACTTAAGCGATGGGATGGGCCGGTGTATAGTCATGGTTGGACTGCCCTATGCTAGCCCTTCCGATGTTGAGTTGATGGAGAGGGTGAAGTACATAGAAGGGTTGGGAAACTCAAATTCCACACAGCTGCCAAATCTTTCACTTAGTAATGAACTTCACGGTGGAGAAGTACGTGCAGGGTTTGACATCCTCAGAAGTTGCAGACGCCGAGGAAAAGATTATTACGAGAATCTTTGCATGAAAGCTGTAAATCAGTCCATCGGTAGAGCAATCCGACACATAAATGACTACGCAGCAATTTTACTAGTTGATACGCGGTACGCAACTGATTCCTCAAAACGGAATTCTTCCCATCCAACTAACAAGCTTCCAAAGTGGATAAAGGATCGCTTCGTTGCTTCAGCTGATTACGGTGAAGTTCATAGGATGTTACATCAGTTCTTTAAACATAACAAGAAGAAATGGCTTCAGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD_2 Helicase_C_2

Protein Analysis

1026

Amino Acids

115.69

Weight (kDa)

7.99

Isoelectric Point (pI)

53.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD_2	PF06733	254 - 296	2.5e-11	DEAD_2
DEAD_2	PF06733	317 - 466	1.2e-33	DEAD_2
Helicase_C_2	PF13307	773 - 982	5e-43	Helicase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005377)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09995 AT1G09995 AT1G09995 AT1G09995 AT1G09995 AT1G79890 AT1G79890 AT1G79890 AT1G79890
fragaria_vesca	FvH4_6g31470 FvH4_6g31470 FvH4_6g31470
malus_domestica	MD09G1210800.v1.1
prunus_persica	Prupe.3G069100_v2.0.a1 Prupe.3G069100_v2.0.a1
pyrus_communis	pycom09g12830 pycom09g12840
rosa_chinensis	RchiOBHm_Chr2g0138331
rosa_laevigata	RLG00000019734 RLG00000019735
rosa_multiflora	Rmu_sc0002475.1_g000005 Rmu_sc0006186.1_g000002
rosa_roxburghii	Rroxscaffold_2G00106330
rosa_rugosa	Rorug02G0351300
rosa_samantha	Rh2AG399400 Rh2BG408700 Rh2CG385900 Rh2DG419300
rosa_wichuraiana	Rw2G032510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1838
Acc16I	TGCGCA	1 cut(s)	1691
Acc36I	ACCTGC	1 cut(s)	742
AccB1I	GGYRCC	2 cut(s)	1049, 1151
AccB7I	CCANNNNNTGG	1 cut(s)	1211
AccII	CGCG	1 cut(s)	2917
AciI	CCGC	7 cut(s)	32, 171, 537, 1539, 1749, 2331, 2917
AclWI	GGATC	3 cut(s)	645, 1941, 2994
AcoI	YGGCCR	1 cut(s)	1602
AcsI	RAATTY	8 cut(s)	44, 446, 731, 1124, 1543, 1792, 2716, 2944
AcuI	CTGAAG	5 cut(s)	1646, 2101, 2391, 2985, 3053
AcyI	GRCGYC	1 cut(s)	2806
AfaI	GTAC	8 cut(s)	62, 1427, 1953, 2288, 2467, 2693, 2772, 2921
AfiI	CCNNNNNNNGG	5 cut(s)	1059, 1211, 1225, 1870, 2940
AflII	CTTAAG	1 cut(s)	2605
AflIII	ACRYGT	1 cut(s)	2470
AgsI	TTSAA	9 cut(s)	226, 323, 961, 1007, 1166, 1478, 1727, 2420, 2510
AhlI	ACTAGT	1 cut(s)	2905
AjnI	CCWGG	5 cut(s)	1456, 1864, 2129, 2213, 2396
Alw21I	GWGCWC	3 cut(s)	271, 1410, 1494
Alw26I	GTCTC	5 cut(s)	1146, 1269, 1296, 1304, 1990
Alw44I	GTGCAC	1 cut(s)	1406
AlwI	GGATC	3 cut(s)	645, 1941, 2994
AlwNI	CAGNNNCTG	1 cut(s)	2339
Ama87I	CYCGRG	2 cut(s)	1497, 2060
AoxI	GGCC	5 cut(s)	96, 124, 468, 1602, 2620
ApaLI	GTGCAC	1 cut(s)	1406
ApeKI	GCWGC	6 cut(s)	867, 1393, 1536, 2043, 2728, 2894
ApoI	RAATTY	8 cut(s)	44, 446, 731, 1124, 1543, 1792, 2716, 2944
ArsI	GACNNNNNNTTYG	2 cut(s)	716, 748
AseI	ATTAAT	1 cut(s)	1608
Asp700I	GAANNNNTTC	2 cut(s)	946, 2481
AspA2I	CCTAGG	1 cut(s)	2456
AspLEI	GCGC	2 cut(s)	1692, 1942
AspS9I	GGNCC	5 cut(s)	97, 468, 1454, 2093, 2620
AsuHPI	GGTGA	5 cut(s)	734, 1763, 2338, 2698, 3026
AsuII	TTCGAA	2 cut(s)	217, 463
AsuNHI	GCTAGC	1 cut(s)	2654
AvaI	CYCGRG	2 cut(s)	1497, 2060
AvaII	GGWCC	2 cut(s)	1454, 2093
AvrII	CCTAGG	1 cut(s)	2456
AxyI	CCTNAGG	1 cut(s)	2340
BaeGI	GKGCMC	2 cut(s)	1410, 1416
BalI	TGGCCA	1 cut(s)	1604
BanI	GGYRCC	2 cut(s)	1049, 1151
BanII	GRGCYC	1 cut(s)	854
BauI	CACGAG	1 cut(s)	1284
BbsI	GAAGAC	3 cut(s)	818, 2347, 2511
Bbv12I	GWGCWC	3 cut(s)	271, 1410, 1494
BbvI	GCAGC	6 cut(s)	854, 1405, 1523, 2055, 2715, 2906
BccI	CCATC	9 cut(s)	341, 707, 1814, 1820, 2606, 2611, 2671, 2867, 2961
BceAI	ACGGC	1 cut(s)	471
BciT130I	CCWGG	5 cut(s)	1458, 1866, 2131, 2215, 2398
BciVI	GTATCC	4 cut(s)	167, 1522, 2286, 2423
BclI	TGATCA	1 cut(s)	1359
BcoDI	GTCTC	5 cut(s)	1146, 1269, 1296, 1304, 1990
BcuI	ACTAGT	1 cut(s)	2905
BfaI	CTAG	5 cut(s)	1346, 2303, 2457, 2655, 2906
BfmI	CTRYAG	2 cut(s)	1977, 2122
BfrI	CTTAAG	1 cut(s)	2605
BfuAI	ACCTGC	1 cut(s)	742
BfuI	GTATCC	4 cut(s)	167, 1522, 2286, 2423
BglI	GCCNNNNNGGC	1 cut(s)	150
BglII	AGATCT	1 cut(s)	1168
BisI	GCNGC	6 cut(s)	868, 1394, 1537, 2044, 2729, 2895
BlnI	CCTAGG	1 cut(s)	2456
BlsI	GCNGC	6 cut(s)	869, 1395, 1538, 2045, 2730, 2896
BmcAI	AGTACT	1 cut(s)	1427
Bme1390I	CCNGG	5 cut(s)	1458, 1866, 2131, 2215, 2398
Bme18I	GGWCC	2 cut(s)	1454, 2093
BmeT110I	CYCGRG	2 cut(s)	1497, 2060
BmgT120I	GGNCC	5 cut(s)	97, 468, 1454, 2093, 2620
BmiI	GGNNCC	7 cut(s)	485, 531, 1051, 1153, 1216, 1230, 1455
BmrFI	CCNGG	5 cut(s)	1458, 1866, 2131, 2215, 2398
BmrI	ACTGGG	2 cut(s)	249, 1914
BmsI	GCATC	9 cut(s)	565, 879, 990, 1079, 1086, 1289, 2355, 2383, 2607
BmtI	GCTAGC	1 cut(s)	2658
BmuI	ACTGGG	2 cut(s)	249, 1914
BpiI	GAAGAC	3 cut(s)	818, 2347, 2511
BpmI	CTGGAG	4 cut(s)	1418, 1593, 2113, 2544
Bpu14I	TTCGAA	2 cut(s)	217, 463
BpuEI	CTTGAG	4 cut(s)	838, 1286, 1360, 2471
Bsa29I	ATCGAT	1 cut(s)	84
BsaAI	YACGTR	1 cut(s)	2774
BsaBI	GATNNNNATC	1 cut(s)	1838
BsaHI	GRCGYC	1 cut(s)	2806
BsaI	GGTCTC	1 cut(s)	1304
BsaXI	ACNNNNNCTCC	4 cut(s)	20, 50, 413, 443
Bsc4I	CCNNNNNNNGG	5 cut(s)	1059, 1211, 1225, 1870, 2940
Bse118I	RCCGGY	2 cut(s)	149, 2622
Bse1I	ACTGG	8 cut(s)	244, 383, 1773, 1909, 1959, 2023, 2075, 2294
Bse21I	CCTNAGG	1 cut(s)	2340
Bse3DI	GCAATG	3 cut(s)	281, 577, 1450
Bse8I	GATNNNNATC	1 cut(s)	1838
BseBI	CCWGG	5 cut(s)	1458, 1866, 2131, 2215, 2398
BseCI	ATCGAT	1 cut(s)	84
BseJI	GATNNNNATC	1 cut(s)	1838
BseLI	CCNNNNNNNGG	5 cut(s)	1059, 1211, 1225, 1870, 2940
BseMI	GCAATG	3 cut(s)	281, 577, 1450
BseMII	CTCAG	3 cut(s)	2331, 2561, 2806
BseNI	ACTGG	8 cut(s)	244, 383, 1773, 1909, 1959, 2023, 2075, 2294
BseRI	GAGGAG	5 cut(s)	41, 434, 539, 542, 611
BseSI	GKGCMC	2 cut(s)	1410, 1416
BseXI	GCAGC	6 cut(s)	854, 1405, 1523, 2055, 2715, 2906
BseYI	CCCAGC	2 cut(s)	49, 1958
BsgI	GTGCAG	2 cut(s)	770, 2796
Bsh1236I	CGCG	1 cut(s)	2917
BshFI	GGCC	5 cut(s)	98, 126, 470, 1604, 2622
BshNI	GGYRCC	2 cut(s)	1049, 1151
BshVI	ATCGAT	1 cut(s)	84
BsiHKAI	GWGCWC	3 cut(s)	271, 1410, 1494
BsiHKCI	CYCGRG	2 cut(s)	1497, 2060
BsiSI	CCGG	4 cut(s)	150, 769, 1053, 2623
BslFI	GGGAC	2 cut(s)	22, 1467
BslI	CCNNNNNNNGG	5 cut(s)	1059, 1211, 1225, 1870, 2940
BsmAI	GTCTC	5 cut(s)	1146, 1269, 1296, 1304, 1990
BsmFI	GGGAC	2 cut(s)	22, 1467
BsmI	GAATGC	2 cut(s)	1722, 2067
BsnI	GGCC	5 cut(s)	98, 126, 470, 1604, 2622
Bso31I	GGTCTC	1 cut(s)	1304
BsoBI	CYCGRG	2 cut(s)	1497, 2060
Bsp119I	TTCGAA	2 cut(s)	217, 463
Bsp1286I	GDGCHC	5 cut(s)	271, 854, 1410, 1416, 1494
BspACI	CCGC	7 cut(s)	32, 171, 537, 1539, 1749, 2331, 2917
BspANI	GGCC	5 cut(s)	98, 126, 470, 1604, 2622
BspCNI	CTCAG	3 cut(s)	2332, 2560, 2805
BspDI	ATCGAT	1 cut(s)	84
BspFNI	CGCG	1 cut(s)	2917
BspHI	TCATGA	2 cut(s)	90, 1552
BspLI	GGNNCC	7 cut(s)	485, 531, 1051, 1153, 1216, 1230, 1455
BspMI	ACCTGC	1 cut(s)	742
BspOI	GCTAGC	1 cut(s)	2658
BspPI	GGATC	3 cut(s)	645, 1941, 2994
BspT104I	TTCGAA	2 cut(s)	217, 463
BspT107I	GGYRCC	2 cut(s)	1049, 1151
BspTI	CTTAAG	1 cut(s)	2605
BspTNI	GGTCTC	1 cut(s)	1304
BsrDI	GCAATG	3 cut(s)	281, 577, 1450
BsrFI	RCCGGY	2 cut(s)	149, 2622
BsrI	ACTGG	8 cut(s)	244, 383, 1773, 1909, 1959, 2023, 2075, 2294
BssAI	RCCGGY	2 cut(s)	149, 2622
BssNI	GRCGYC	1 cut(s)	2806
BssSI	CACGAG	1 cut(s)	1284
BssT1I	CCWWGG	3 cut(s)	1155, 2006, 2456
Bst2BI	CACGAG	1 cut(s)	1284
Bst2UI	CCWGG	5 cut(s)	1458, 1866, 2131, 2215, 2398
Bst4CI	ACNGT	7 cut(s)	230, 792, 1257, 2158, 2246, 2762, 3014
Bst6I	CTCTTC	9 cut(s)	6, 18, 170, 621, 1272, 1749, 2006, 2368, 2456
BstACI	GRCGYC	1 cut(s)	2806
BstAFI	CTTAAG	1 cut(s)	2605
BstBAI	YACGTR	1 cut(s)	2774
BstBI	TTCGAA	2 cut(s)	217, 463
BstC8I	GCNNGC	2 cut(s)	1944, 2656
BstDEI	CTNAG	9 cut(s)	251, 332, 1045, 1100, 1520, 2340, 2547, 2744, 2792
BstDSI	CCRYGG	2 cut(s)	487, 2089
BstEII	GGTNACC	2 cut(s)	2010, 2326
BstFNI	CGCG	1 cut(s)	2917
BstHHI	GCGC	2 cut(s)	1692, 1942
BstMAI	GTCTC	5 cut(s)	1146, 1269, 1296, 1304, 1990
BstMWI	GCNNNNNNNGC	4 cut(s)	150, 1221, 1574, 2402
BstNI	CCWGG	5 cut(s)	1458, 1866, 2131, 2215, 2398
BstNSI	RCATGY	4 cut(s)	1369, 1863, 1946, 2474
BstPI	GGTNACC	2 cut(s)	2010, 2326
BstSCI	CCNGG	5 cut(s)	1456, 1864, 2129, 2213, 2396
BstSFI	CTRYAG	2 cut(s)	1977, 2122
BstSLI	GKGCMC	2 cut(s)	1410, 1416
BstUI	CGCG	1 cut(s)	2917
BstV1I	GCAGC	6 cut(s)	854, 1405, 1523, 2055, 2715, 2906
BstV2I	GAAGAC	3 cut(s)	818, 2347, 2511
BstX2I	RGATCY	2 cut(s)	637, 1168
BstYI	RGATCY	2 cut(s)	637, 1168
Bsu15I	ATCGAT	1 cut(s)	84
Bsu36I	CCTNAGG	1 cut(s)	2340
BsuI	GTATCC	4 cut(s)	167, 1522, 2286, 2423
BsuRI	GGCC	5 cut(s)	98, 126, 470, 1604, 2622
BsuTUI	ATCGAT	1 cut(s)	84
BtgI	CCRYGG	2 cut(s)	487, 2089
BtgZI	GCGATG	1 cut(s)	2625
BtsIMutI	CAGTG	3 cut(s)	894, 1805, 2015
BveI	ACCTGC	1 cut(s)	742
Cac8I	GCNNGC	2 cut(s)	1944, 2656
CaiI	CAGNNNCTG	1 cut(s)	2339
CciI	TCATGA	2 cut(s)	90, 1552
CfoI	GCGC	2 cut(s)	1692, 1942
Cfr10I	RCCGGY	2 cut(s)	149, 2622
Cfr13I	GGNCC	5 cut(s)	97, 468, 1454, 2093, 2620
ClaI	ATCGAT	1 cut(s)	84
CseI	GACGC	2 cut(s)	2429, 2814
CsiI	ACCWGGT	1 cut(s)	1456
Csp6I	GTAC	8 cut(s)	61, 1426, 1952, 2287, 2466, 2692, 2771, 2920
CspCI	CAANNNNNGTGG	2 cut(s)	1681, 1716
CviQI	GTAC	8 cut(s)	61, 1426, 1952, 2287, 2466, 2692, 2771, 2920
DdeI	CTNAG	9 cut(s)	251, 332, 1045, 1100, 1520, 2340, 2547, 2744, 2792
DraI	TTTAAA	3 cut(s)	2188, 2197, 3046
EaeI	YGGCCR	1 cut(s)	1602
Eam1104I	CTCTTC	9 cut(s)	6, 18, 170, 621, 1272, 1749, 2006, 2368, 2456
EarI	CTCTTC	9 cut(s)	6, 18, 170, 621, 1272, 1749, 2006, 2368, 2456
EciI	GGCGGA	1 cut(s)	47
Eco130I	CCWWGG	3 cut(s)	1155, 2006, 2456
Eco147I	AGGCCT	1 cut(s)	126
Eco24I	GRGCYC	1 cut(s)	854
Eco31I	GGTCTC	1 cut(s)	1304
Eco32I	GATATC	1 cut(s)	2589
Eco47I	GGWCC	2 cut(s)	1454, 2093
Eco57I	CTGAAG	5 cut(s)	1646, 2101, 2391, 2985, 3053
Eco81I	CCTNAGG	1 cut(s)	2340
Eco88I	CYCGRG	2 cut(s)	1497, 2060
Eco91I	GGTNACC	2 cut(s)	2010, 2326
EcoO109I	RGGNCCY	2 cut(s)	97, 1454
EcoO65I	GGTNACC	2 cut(s)	2010, 2326
EcoRI	GAATTC	2 cut(s)	1124, 2944
EcoRII	CCWGG	5 cut(s)	1456, 1864, 2129, 2213, 2396
EcoRV	GATATC	1 cut(s)	2589
EcoT14I	CCWWGG	3 cut(s)	1155, 2006, 2456
EcoT38I	GRGCYC	1 cut(s)	854
ErhI	CCWWGG	3 cut(s)	1155, 2006, 2456
FalI	AAGNNNNNCTT	8 cut(s)	111, 143, 803, 835, 1369, 1401, 2975, 3007
FaqI	GGGAC	2 cut(s)	22, 1467
FbaI	TGATCA	1 cut(s)	1359
Fnu4HI	GCNGC	6 cut(s)	868, 1394, 1537, 2044, 2729, 2895
FriOI	GRGCYC	1 cut(s)	854
Fsp4HI	GCNGC	6 cut(s)	868, 1394, 1537, 2044, 2729, 2895
FspBI	CTAG	5 cut(s)	1346, 2303, 2457, 2655, 2906
FspI	TGCGCA	1 cut(s)	1691
GlaI	GCGC	2 cut(s)	1691, 1941
GluI	GCNGC	6 cut(s)	868, 1394, 1537, 2044, 2729, 2895
GsaI	CCCAGC	2 cut(s)	53, 1962
GsuI	CTGGAG	4 cut(s)	1418, 1593, 2113, 2544
HaeIII	GGCC	5 cut(s)	98, 126, 470, 1604, 2622
HapII	CCGG	4 cut(s)	150, 769, 1053, 2623
HgaI	GACGC	2 cut(s)	2429, 2814
HhaI	GCGC	2 cut(s)	1692, 1942
Hin1I	GRCGYC	1 cut(s)	2806
Hin6I	GCGC	2 cut(s)	1690, 1940
HinP1I	GCGC	2 cut(s)	1690, 1940
HincII	GTYRAC	1 cut(s)	358
HindII	GTYRAC	1 cut(s)	358
HindIII	AAGCTT	2 cut(s)	2403, 2966
HpaII	CCGG	4 cut(s)	150, 769, 1053, 2623
HphI	GGTGA	5 cut(s)	734, 1763, 2338, 2698, 3026
Hpy166II	GTNNAC	3 cut(s)	358, 1408, 2468
Hpy8I	GTNNAC	3 cut(s)	358, 1408, 2468
Hpy99I	CGWCG	1 cut(s)	2579
HpyCH4III	ACNGT	7 cut(s)	230, 792, 1257, 2158, 2246, 2762, 3014
HpyCH4IV	ACGT	1 cut(s)	2773
HpyF10VI	GCNNNNNNNGC	4 cut(s)	150, 1221, 1574, 2402
HpyF3I	CTNAG	9 cut(s)	251, 332, 1045, 1100, 1520, 2340, 2547, 2744, 2792
HpySE526I	ACGT	1 cut(s)	2773
Hsp92I	GRCGYC	1 cut(s)	2806
HspAI	GCGC	2 cut(s)	1690, 1940
Ksp22I	TGATCA	1 cut(s)	1359
LmnI	GCTCC	7 cut(s)	483, 529, 1131, 1220, 1574, 2132, 2299
Lsp1109I	GCAGC	6 cut(s)	854, 1405, 1523, 2055, 2715, 2906
LweI	GCATC	9 cut(s)	565, 879, 990, 1079, 1086, 1289, 2355, 2383, 2607
MabI	ACCWGGT	1 cut(s)	1456
MaeI	CTAG	5 cut(s)	1346, 2303, 2457, 2655, 2906
MaeII	ACGT	1 cut(s)	2773
MaeIII	GTNAC	6 cut(s)	722, 925, 2010, 2102, 2326, 3030
MfeI	CAATTG	1 cut(s)	2505
MflI	RGATCY	2 cut(s)	637, 1168
MhlI	GDGCHC	5 cut(s)	271, 854, 1410, 1416, 1494
MlsI	TGGCCA	1 cut(s)	1604
MluNI	TGGCCA	1 cut(s)	1604
MlyI	GAGTC	7 cut(s)	44, 353, 1065, 1298, 1346, 1367, 1992
MmeI	TCCRAC	5 cut(s)	1880, 1996, 2620, 2899, 2981
Mox20I	TGGCCA	1 cut(s)	1604
MroXI	GAANNNNTTC	2 cut(s)	946, 2481
MscI	TGGCCA	1 cut(s)	1604
MslI	CAYNNNNRTG	1 cut(s)	2883
Msp20I	TGGCCA	1 cut(s)	1604
MspA1I	CMGCKG	3 cut(s)	1396, 2728, 3005
MspCI	CTTAAG	1 cut(s)	2605
MspI	CCGG	4 cut(s)	150, 769, 1053, 2623
MspR9I	CCNGG	5 cut(s)	1458, 1866, 2131, 2215, 2398
MunI	CAATTG	1 cut(s)	2505
Mva1269I	GAATGC	2 cut(s)	1722, 2067
MvaI	CCWGG	5 cut(s)	1458, 1866, 2131, 2215, 2398
MvnI	CGCG	1 cut(s)	2917
MwoI	GCNNNNNNNGC	4 cut(s)	150, 1221, 1574, 2402
NheI	GCTAGC	1 cut(s)	2654
NlaIV	GGNNCC	7 cut(s)	485, 531, 1051, 1153, 1216, 1230, 1455
NmeAIII	GCCGAG	1 cut(s)	2834
NmuCI	GTSAC	3 cut(s)	722, 2102, 2326
NsbI	TGCGCA	1 cut(s)	1691
NspI	RCATGY	4 cut(s)	1369, 1863, 1946, 2474
NspV	TTCGAA	2 cut(s)	217, 463
PaeI	GCATGC	1 cut(s)	1946
PagI	TCATGA	2 cut(s)	90, 1552
PasI	CCCWGGG	1 cut(s)	2214
PceI	AGGCCT	1 cut(s)	126
PciI	ACATGT	1 cut(s)	2470
PctI	GAATGC	2 cut(s)	1722, 2067
PdmI	GAANNNNTTC	2 cut(s)	946, 2481
PfeI	GAWTC	7 cut(s)	323, 1063, 1380, 2282, 2477, 2830, 2930
PflMI	CCANNNNNTGG	1 cut(s)	1211
PfoI	TCCNGGA	1 cut(s)	2129
PkrI	GCNGC	6 cut(s)	869, 1395, 1538, 2045, 2730, 2896
PleI	GAGTC	7 cut(s)	43, 353, 1064, 1297, 1346, 1367, 1992
PpsI	GAGTC	7 cut(s)	43, 353, 1064, 1297, 1346, 1367, 1992
Ppu21I	YACGTR	1 cut(s)	2774
PpuMI	RGGWCCY	1 cut(s)	1454
PscI	ACATGT	1 cut(s)	2470
PshBI	ATTAAT	1 cut(s)	1608
PsiI	TTATAA	1 cut(s)	1838
Psp5II	RGGWCCY	1 cut(s)	1454
Psp6I	CCWGG	5 cut(s)	1456, 1864, 2129, 2213, 2396
PspEI	GGTNACC	2 cut(s)	2010, 2326
PspFI	CCCAGC	2 cut(s)	49, 1958
PspGI	CCWGG	5 cut(s)	1456, 1864, 2129, 2213, 2396
PspN4I	GGNNCC	7 cut(s)	485, 531, 1051, 1153, 1216, 1230, 1455
PspPI	GGNCC	5 cut(s)	97, 468, 1454, 2093, 2620
PspPPI	RGGWCCY	1 cut(s)	1454
PsrI	GAACNNNNNNTAC	2 cut(s)	2466, 2498
PstNI	CAGNNNCTG	1 cut(s)	2339
PsuI	RGATCY	2 cut(s)	637, 1168
PvuII	CAGCTG	3 cut(s)	1396, 2728, 3005
RsaI	GTAC	8 cut(s)	62, 1427, 1953, 2288, 2467, 2693, 2772, 2921
RsaNI	GTAC	8 cut(s)	61, 1426, 1952, 2287, 2466, 2692, 2771, 2920
RseI	CAYNNNNRTG	1 cut(s)	2883
SatI	GCNGC	6 cut(s)	868, 1394, 1537, 2044, 2729, 2895
Sau96I	GGNCC	5 cut(s)	97, 468, 1454, 2093, 2620
ScaI	AGTACT	1 cut(s)	1427
SchI	GAGTC	7 cut(s)	44, 353, 1065, 1298, 1346, 1367, 1992
ScrFI	CCNGG	5 cut(s)	1458, 1866, 2131, 2215, 2398
SduI	GDGCHC	5 cut(s)	271, 854, 1410, 1416, 1494
SexAI	ACCWGGT	1 cut(s)	1456
SfaNI	GCATC	9 cut(s)	565, 879, 990, 1079, 1086, 1289, 2355, 2383, 2607
SfcI	CTRYAG	2 cut(s)	1977, 2122
SfuI	TTCGAA	2 cut(s)	217, 463
SinI	GGWCC	2 cut(s)	1454, 2093
SmiMI	CAYNNNNRTG	1 cut(s)	2883
SmlI	CTYRAG	5 cut(s)	853, 1301, 1375, 2486, 2605
SmoI	CTYRAG	5 cut(s)	853, 1301, 1375, 2486, 2605
SpeI	ACTAGT	1 cut(s)	2905
SphI	GCATGC	1 cut(s)	1946
SseBI	AGGCCT	1 cut(s)	126
SsiI	CCGC	7 cut(s)	32, 171, 537, 1539, 1749, 2331, 2917
SspI	AATATT	3 cut(s)	1318, 1915, 2166
SspMI	CTAG	5 cut(s)	1346, 2303, 2457, 2655, 2906
StuI	AGGCCT	1 cut(s)	126
StyD4I	CCNGG	5 cut(s)	1456, 1864, 2129, 2213, 2396
StyI	CCWWGG	3 cut(s)	1155, 2006, 2456
TaaI	ACNGT	7 cut(s)	230, 792, 1257, 2158, 2246, 2762, 3014
TaiI	ACGT	1 cut(s)	2776
TaqI	TCGA	7 cut(s)	84, 138, 217, 233, 352, 463, 2238
TatI	WGTACW	4 cut(s)	1425, 1951, 2465, 2691
TfiI	GAWTC	7 cut(s)	323, 1063, 1380, 2282, 2477, 2830, 2930
TscAI	CASTG	3 cut(s)	901, 1812, 2022
TseFI	GTSAC	3 cut(s)	722, 2102, 2326
TseI	GCWGC	6 cut(s)	867, 1393, 1536, 2043, 2728, 2894
Tsp45I	GTSAC	3 cut(s)	722, 2102, 2326
TspGWI	ACGGA	3 cut(s)	48, 201, 2956
TspRI	CASTG	3 cut(s)	901, 1812, 2022
Van91I	CCANNNNNTGG	1 cut(s)	1211
Vha464I	CTTAAG	1 cut(s)	2605
VneI	GTGCAC	1 cut(s)	1406
VpaK11BI	GGWCC	2 cut(s)	1454, 2093
VspI	ATTAAT	1 cut(s)	1608
XapI	RAATTY	8 cut(s)	44, 446, 731, 1124, 1543, 1792, 2716, 2944
XceI	RCATGY	4 cut(s)	1369, 1863, 1946, 2474
XmaJI	CCTAGG	1 cut(s)	2456
XmnI	GAANNNNTTC	2 cut(s)	946, 2481
XspI	CTAG	5 cut(s)	1346, 2303, 2457, 2655, 2906
ZrmI	AGTACT	1 cut(s)	1427

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.