RLG00000019735

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005377
Physical Location & Seq
Reverse (-)
56120813 .. 56122824
2012 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019735

Sequence Viewer

Length: 906 bp
ATGGAAGGAGAAAACCAAAATGGGGAGGCGCCCCCAAAATTCCCAGCATTTCCTTACAAACCCTACTCCATCCAGATCGACTTCATGAACGCCCTTTACCACTCTCTCAACCAAGGCGGCCTCTCCATGCTCGAAAGCCCCACCGGGACTGGTAAAACTCTAAGTATCATTTGTAGTGCTTTGCAATGGGTGGTCGATAAGAGGGAGCAAGAGAAGTTGAAAAAAGAGGTTGAAACTGACAATAGTTGTGCTAAAGGCGGTGAATTTGGCTCGGATGATGAACCTGATTGGATGAGGAATGCTGTTATTAACAAAGACAATCAGGGTGAGGAGAAGAAGTCTGGGAAGAAGGATAAATTTGGGAGTGGATTTCGCAGGGCCGATAAGAGAAAGAACCATGCAAATTGTACTGATTTGTTCTCTCGGCGTGTTGTGGAAGAGTCTTGCACAAACAAGGAGGGAAAGAGTTTGCAAGTGAGCAGTGATGCTGTGGATTTGAATGATGAGGAGGAGTTTTTGCTGGAAGAACATGAGAGTGATGAGGAAGGTGTTCCGTGTGCTGGGAAGTCGAAGAGGAAGGTTTCTGGGGTTTCTCTTAGTTTGTCAAGTGACGAGGAAGATGGAGCAGAGGGGTCTAGTGATGATGAAGATGAGGGGGAAGAGAAGTTGAAGGTTTATTTTTGTAGTCGGACACATTCACAGCTTTCGCAGTTCATAAAGGAGTTGAGAAAGACAGTCTTTGCTAATGAGTTGAAGGTGGTTTGTTTGGGCTCAAGGAAGAATTTCTGCATTAACCAAGAGGTATTGAAACTTGGAAACTCAACTCGCATCAATGAACGCTGTTTGGAACTTCAGAAAAACAAGAATAAGGAAGTTTCTAAAATCAAGGGGATTTCTCTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD_2 Helicase_C_2

Protein Analysis

302

Amino Acids

33.68

Weight (kDa)

5.22

Isoelectric Point (pI)

41.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD_2 PF06733 227 - 291 2.3e-18 DEAD_2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 28
AciI CCGC 2 cut(s) 117, 258
AcsI RAATTY 4 cut(s) 38, 263, 356, 781
AcuI CTGAAG 1 cut(s) 836
AcyI GRCGYC 1 cut(s) 29
AfaI GTAC 1 cut(s) 409
AfiI CCNNNNNNNGG 2 cut(s) 22, 560
AgsI TTSAA 6 cut(s) 220, 233, 499, 670, 754, 808
AluBI AGCT 1 cut(s) 703
AluI AGCT 1 cut(s) 703
AoxI GGCC 2 cut(s) 118, 378
ApoI RAATTY 4 cut(s) 38, 263, 356, 781
Asp700I GAANNNNTTC 2 cut(s) 549, 782
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 378
AsuC2I CCSGG 1 cut(s) 145
AsuHPI GGTGA 2 cut(s) 272, 338
BanI GGYRCC 1 cut(s) 28
BanII GRGCYC 1 cut(s) 773
BccI CCATC 2 cut(s) 77, 614
BcnI CCSGG 1 cut(s) 145
BfaI CTAG 1 cut(s) 636
BfoI RGCGCY 1 cut(s) 32
BisI GCNGC 1 cut(s) 118
BlsI GCNGC 1 cut(s) 119
Bme1390I CCNGG 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 378
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 1 cut(s) 145
BmsI GCATC 2 cut(s) 475, 837
BpuEI CTTGAG 1 cut(s) 757
BpuMI CCSGG 1 cut(s) 145
BsaHI GRCGYC 1 cut(s) 29
BsaJI CCNNGG 1 cut(s) 112
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 560
Bse1I ACTGG 1 cut(s) 154
Bse3DI GCAATG 1 cut(s) 191
BseDI CCNNGG 1 cut(s) 112
BseGI GGATG 3 cut(s) 69, 280, 297
BseLI CCNNNNNNNGG 2 cut(s) 22, 560
BseMI GCAATG 1 cut(s) 191
BseNI ACTGG 1 cut(s) 154
BseRI GAGGAG 3 cut(s) 344, 521, 524
BseYI CCCAGC 2 cut(s) 43, 560
BshFI GGCC 2 cut(s) 120, 380
BshNI GGYRCC 1 cut(s) 28
BsiSI CCGG 1 cut(s) 144
BslFI GGGAC 1 cut(s) 160
BslI CCNNNNNNNGG 2 cut(s) 22, 560
BsmFI GGGAC 1 cut(s) 160
BsmI GAATGC 1 cut(s) 304
BsnI GGCC 2 cut(s) 120, 380
Bsp1286I GDGCHC 1 cut(s) 773
Bsp143I GATC 1 cut(s) 75
BspACI CCGC 2 cut(s) 117, 258
BspANI GGCC 2 cut(s) 120, 380
BspHI TCATGA 1 cut(s) 84
BspLI GGNNCC 1 cut(s) 30
BspT107I GGYRCC 1 cut(s) 28
BsrDI GCAATG 1 cut(s) 191
BsrI ACTGG 1 cut(s) 154
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 1 cut(s) 75
BssNI GRCGYC 1 cut(s) 29
BssT1I CCWWGG 1 cut(s) 112
Bst4CI ACNGT 1 cut(s) 736
Bst6I CTCTTC 3 cut(s) 432, 566, 654
BstACI GRCGYC 1 cut(s) 29
BstDEI CTNAG 2 cut(s) 161, 596
BstF5I GGATG 3 cut(s) 69, 280, 297
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 1 cut(s) 78
BstMBI GATC 1 cut(s) 75
BstSCI CCNGG 1 cut(s) 143
BsuRI GGCC 2 cut(s) 120, 380
BtsCI GGATG 3 cut(s) 69, 280, 297
BtsI GCAGTG 1 cut(s) 487
BtsIMutI CAGTG 1 cut(s) 487
CciI TCATGA 1 cut(s) 84
CfoI GCGC 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 378
Csp6I GTAC 1 cut(s) 408
CviAII CATG 4 cut(s) 85, 127, 398, 530
CviJI RGCY 6 cut(s) 120, 138, 270, 380, 703, 771
CviKI_1 RGCY 6 cut(s) 120, 138, 270, 380, 703, 771
CviQI GTAC 1 cut(s) 408
DdeI CTNAG 2 cut(s) 161, 596
DinI GGCGCC 1 cut(s) 30
DpnI GATC 1 cut(s) 77
DpnII GATC 1 cut(s) 75
Eam1104I CTCTTC 3 cut(s) 432, 566, 654
EarI CTCTTC 3 cut(s) 432, 566, 654
Eco130I CCWWGG 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 773
Eco57I CTGAAG 1 cut(s) 836
EcoT14I CCWWGG 1 cut(s) 112
EcoT38I GRGCYC 1 cut(s) 773
EgeI GGCGCC 1 cut(s) 30
EheI GGCGCC 1 cut(s) 30
ErhI CCWWGG 1 cut(s) 112
FaeI CATG 4 cut(s) 88, 130, 401, 533
FaiI YATR 5 cut(s) 86, 128, 399, 531, 716
FalI AAGNNNNNCTT 2 cut(s) 722, 754
FaqI GGGAC 1 cut(s) 160
FatI CATG 4 cut(s) 84, 126, 397, 529
Fnu4HI GCNGC 1 cut(s) 118
FokI GGATG 3 cut(s) 56, 287, 304
FriOI GRGCYC 1 cut(s) 773
Fsp4HI GCNGC 1 cut(s) 118
FspBI CTAG 1 cut(s) 636
GlaI GCGC 1 cut(s) 30
GluI GCNGC 1 cut(s) 118
GsaI CCCAGC 2 cut(s) 47, 564
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 2 cut(s) 120, 380
HapII CCGG 1 cut(s) 144
HhaI GCGC 1 cut(s) 31
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 4 cut(s) 88, 130, 401, 533
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HinfI GANTC 1 cut(s) 440
HpaII CCGG 1 cut(s) 144
HphI GGTGA 2 cut(s) 272, 338
Hpy188I TCNGA 3 cut(s) 274, 690, 855
Hpy188III TCNNGA 3 cut(s) 73, 85, 899
HpyAV CCTTC 5 cut(s) 343, 539, 571, 664, 748
HpyCH4III ACNGT 1 cut(s) 736
HpyCH4V TGCA 5 cut(s) 184, 401, 447, 472, 789
HpyF3I CTNAG 2 cut(s) 161, 596
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 4 cut(s) 88, 130, 401, 533
HspAI GCGC 1 cut(s) 29
KasI GGCGCC 1 cut(s) 28
Kzo9I GATC 1 cut(s) 75
LmnI GCTCC 2 cut(s) 205, 623
LweI GCATC 2 cut(s) 475, 837
MaeI CTAG 1 cut(s) 636
MaeIII GTNAC 1 cut(s) 608
MalI GATC 1 cut(s) 77
MboI GATC 1 cut(s) 75
MboII GAAGA 9 cut(s) 346, 358, 449, 536, 583, 629, 659, 671, 790
MhlI GDGCHC 1 cut(s) 773
MluCI AATT 5 cut(s) 38, 263, 356, 403, 781
Mly113I GGCGCC 1 cut(s) 29
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 1 cut(s) 668
MroXI GAANNNNTTC 2 cut(s) 549, 782
MseI TTAA 2 cut(s) 309, 792
MslI CAYNNNNRTG 1 cut(s) 534
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 1 cut(s) 145
Mva1269I GAATGC 1 cut(s) 304
NarI GGCGCC 1 cut(s) 29
NciI CCSGG 1 cut(s) 145
NdeII GATC 1 cut(s) 75
NlaIII CATG 4 cut(s) 88, 130, 401, 533
NlaIV GGNNCC 1 cut(s) 30
NmeAIII GCCGAG 1 cut(s) 403
NmuCI GTSAC 1 cut(s) 608
PagI TCATGA 1 cut(s) 84
PctI GAATGC 1 cut(s) 304
PdmI GAANNNNTTC 2 cut(s) 549, 782
PkrI GCNGC 1 cut(s) 119
PleI GAGTC 1 cut(s) 448
PluTI GGCGCC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 448
PspFI CCCAGC 2 cut(s) 43, 560
PspN4I GGNNCC 1 cut(s) 30
PspPI GGNCC 1 cut(s) 378
PsrI GAACNNNNNNTAC 2 cut(s) 80, 112
RsaI GTAC 1 cut(s) 409
RsaNI GTAC 1 cut(s) 408
RseI CAYNNNNRTG 1 cut(s) 534
SaqAI TTAA 2 cut(s) 309, 792
SatI GCNGC 1 cut(s) 118
Sau3AI GATC 1 cut(s) 75
Sau96I GGNCC 1 cut(s) 378
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 1 cut(s) 145
SduI GDGCHC 1 cut(s) 773
SetI ASST 8 cut(s) 231, 286, 550, 582, 675, 705, 759, 804
SfaNI GCATC 2 cut(s) 475, 837
SfoI GGCGCC 1 cut(s) 30
SmiMI CAYNNNNRTG 1 cut(s) 534
SmlI CTYRAG 1 cut(s) 772
SmoI CTYRAG 1 cut(s) 772
Sse9I AATT 5 cut(s) 38, 263, 356, 403, 781
SsiI CCGC 2 cut(s) 117, 258
SspDI GGCGCC 1 cut(s) 28
SspMI CTAG 1 cut(s) 636
StyD4I CCNGG 1 cut(s) 143
StyI CCWWGG 1 cut(s) 112
TaaI ACNGT 1 cut(s) 736
TaqI TCGA 4 cut(s) 78, 132, 195, 569
TasI AATT 5 cut(s) 38, 263, 356, 403, 781
TatI WGTACW 1 cut(s) 407
TauI GCSGC 1 cut(s) 120
Tru1I TTAA 2 cut(s) 309, 792
Tru9I TTAA 2 cut(s) 309, 792
TscAI CASTG 1 cut(s) 487
TseFI GTSAC 1 cut(s) 608
Tsp45I GTSAC 1 cut(s) 608
TspDTI ATGAA 6 cut(s) 73, 101, 294, 660, 703, 849
TspGWI ACGGA 1 cut(s) 543
TspRI CASTG 1 cut(s) 487
XapI RAATTY 4 cut(s) 38, 263, 356, 781
XmnI GAANNNNTTC 2 cut(s) 549, 782
XspI CTAG 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.