Rorug02G0351300

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005377
Physical Location & Seq
Reverse (-)
44144914 .. 44148799
3886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0351300.1

Sequence Viewer

Length: 624 bp
ATGTTCAGGAGAAGGAGAAGGTGTTGGTGGAGTATTGTAAGGCCGCCAATGGCCTCGAGAAGGTCGTTCTCAAAGAGCTCCGTGGCTCTTCCGCTGAGGTGTATTTATATGGGGCCCAAGTTTCATCGTGGAAAAATGAACACGGGGAAGAATTTCGTGAGCGGTAAGGCTACATTTAAGTCTCCTAAAGCAATCCGTGGAGGAATTCCGATCTGTTTTCCACAATTTGGAAATCTTGGTTCTCTTGAACAACATGGATTTGCAAGGAATAGGTTTTGGAGTATTGATCAAGATCCACCCCCTCATCCAACAAATAGTGGCAACAAGGCTTTTATTGATCTAATCCTGAAGCCCTCTGAAGAAGATTCAAAGATATGGCCTCACAAATATGAATTTCGGCTTAGGGTAACCCTGGGACCTGGAGGAGATTTGATGTTGAATTCCCGCATTCGAAATACAAACACTGATGGAAAGTCGTTTACATTCACATTCGCTTATCACACATATTTGTCTGTAATTGATATCAGTGAAGTGCGAGTAGAAGGATTAGAGACACTAGATTATCTGGATAACTTGAAGGATAGAAAGCGGTTTACTGAACAAGGGGATGCCATAACGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD_2 Helicase_C_2

Protein Analysis

207

Amino Acids

23.85

Weight (kDa)

10.13

Isoelectric Point (pI)

51.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldose_epim PF01263 57 - 207 1.7e-32 Aldose 1-epimerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 227
AccBSI CCGCTC 1 cut(s) 162
AciI CCGC 5 cut(s) 44, 92, 162, 445, 589
AclI AACGTT 1 cut(s) 617
AclWI GGATC 1 cut(s) 287
AcsI RAATTY 4 cut(s) 151, 204, 392, 439
AcuI CTGAAG 2 cut(s) 368, 378
AfiI CCNNNNNNNGG 2 cut(s) 60, 227
AgsI TTSAA 4 cut(s) 248, 369, 439, 577
AjnI CCWGG 2 cut(s) 411, 418
AluBI AGCT 1 cut(s) 78
AluI AGCT 1 cut(s) 78
Alw21I GWGCWC 1 cut(s) 80
Alw26I GTCTC 2 cut(s) 186, 545
AlwI GGATC 1 cut(s) 287
Ama87I CYCGRG 1 cut(s) 55
AoxI GGCC 4 cut(s) 41, 51, 113, 377
ApaI GGGCCC 1 cut(s) 117
ApoI RAATTY 4 cut(s) 151, 204, 392, 439
Asp700I GAANNNNTTC 1 cut(s) 152
AspS9I GGNCC 3 cut(s) 113, 114, 416
AsuII TTCGAA 1 cut(s) 451
AvaI CYCGRG 1 cut(s) 55
AvaII GGWCC 1 cut(s) 416
BaeGI GKGCMC 1 cut(s) 117
BanII GRGCYC 2 cut(s) 80, 117
Bbv12I GWGCWC 1 cut(s) 80
BbvCI CCTCAGC 1 cut(s) 95
BccI CCATC 1 cut(s) 461
BciT130I CCWGG 2 cut(s) 413, 420
BclI TGATCA 1 cut(s) 286
BcoDI GTCTC 2 cut(s) 186, 545
BfaI CTAG 1 cut(s) 557
BisI GCNGC 1 cut(s) 44
BlsI GCNGC 1 cut(s) 45
Bme1390I CCNGG 2 cut(s) 413, 420
Bme18I GGWCC 1 cut(s) 416
BmeT110I CYCGRG 1 cut(s) 55
BmgT120I GGNCC 3 cut(s) 113, 114, 416
BmiI GGNNCC 3 cut(s) 114, 115, 417
BmrFI CCNGG 2 cut(s) 413, 420
BmsI GCATC 1 cut(s) 598
BpmI CTGGAG 1 cut(s) 441
Bpu10I CCTNAGC 2 cut(s) 95, 401
Bpu14I TTCGAA 1 cut(s) 451
BsaBI GATNNNNATC 1 cut(s) 291
BsaJI CCNNGG 4 cut(s) 81, 196, 411, 412
BsaXI ACNNNNNCTCC 2 cut(s) 7, 37
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 227
Bse8I GATNNNNATC 1 cut(s) 291
BseBI CCWGG 2 cut(s) 413, 420
BseDI CCNNGG 4 cut(s) 81, 196, 411, 412
BseGI GGATG 2 cut(s) 304, 613
BseJI GATNNNNATC 1 cut(s) 291
BseLI CCNNNNNNNGG 2 cut(s) 60, 227
BseMII CTCAG 1 cut(s) 86
BseRI GAGGAG 1 cut(s) 438
BseSI GKGCMC 1 cut(s) 117
BshFI GGCC 4 cut(s) 43, 53, 115, 379
BsiHKAI GWGCWC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 55
BslFI GGGAC 1 cut(s) 429
BslI CCNNNNNNNGG 2 cut(s) 60, 227
BsmAI GTCTC 2 cut(s) 186, 545
BsmFI GGGAC 1 cut(s) 429
BsmI GAATGC 1 cut(s) 447
BsnI GGCC 4 cut(s) 43, 53, 115, 379
BsoBI CYCGRG 1 cut(s) 55
Bsp119I TTCGAA 1 cut(s) 451
Bsp120I GGGCCC 1 cut(s) 113
Bsp1286I GDGCHC 2 cut(s) 80, 117
Bsp143I GATC 4 cut(s) 210, 286, 292, 337
BspACI CCGC 5 cut(s) 44, 92, 162, 445, 589
BspANI GGCC 4 cut(s) 43, 53, 115, 379
BspCNI CTCAG 1 cut(s) 87
BspLI GGNNCC 3 cut(s) 114, 115, 417
BspPI GGATC 1 cut(s) 287
BspQI GCTCTTC 1 cut(s) 93
BspT104I TTCGAA 1 cut(s) 451
BsrBI CCGCTC 1 cut(s) 162
BssECI CCNNGG 4 cut(s) 81, 196, 411, 412
BssMI GATC 4 cut(s) 210, 286, 292, 337
Bst2UI CCWGG 2 cut(s) 413, 420
Bst6I CTCTTC 1 cut(s) 93
BstBI TTCGAA 1 cut(s) 451
BstDEI CTNAG 2 cut(s) 95, 401
BstDSI CCRYGG 2 cut(s) 81, 196
BstEII GGTNACC 1 cut(s) 406
BstENI CCTNNNNNAGG 1 cut(s) 58
BstF5I GGATG 2 cut(s) 304, 613
BstKTI GATC 4 cut(s) 213, 289, 295, 340
BstMAI GTCTC 2 cut(s) 186, 545
BstMBI GATC 4 cut(s) 210, 286, 292, 337
BstNI CCWGG 2 cut(s) 413, 420
BstPI GGTNACC 1 cut(s) 406
BstSCI CCNGG 2 cut(s) 411, 418
BstSLI GKGCMC 1 cut(s) 117
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
BsuRI GGCC 4 cut(s) 43, 53, 115, 379
BtgI CCRYGG 2 cut(s) 81, 196
BtsCI GGATG 2 cut(s) 304, 613
BtsIMutI CAGTG 2 cut(s) 462, 532
Cfr13I GGNCC 3 cut(s) 113, 114, 416
CviAII CATG 1 cut(s) 254
DdeI CTNAG 2 cut(s) 95, 401
DpnI GATC 4 cut(s) 212, 288, 294, 339
DpnII GATC 4 cut(s) 210, 286, 292, 337
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
Ecl136II GAGCTC 1 cut(s) 78
Eco24I GRGCYC 2 cut(s) 80, 117
Eco32I GATATC 1 cut(s) 523
Eco47I GGWCC 1 cut(s) 416
Eco53kI GAGCTC 1 cut(s) 78
Eco57I CTGAAG 2 cut(s) 368, 378
Eco88I CYCGRG 1 cut(s) 55
Eco91I GGTNACC 1 cut(s) 406
EcoICRI GAGCTC 1 cut(s) 78
EcoNI CCTNNNNNAGG 1 cut(s) 58
EcoO109I RGGNCCY 2 cut(s) 113, 416
EcoO65I GGTNACC 1 cut(s) 406
EcoRI GAATTC 2 cut(s) 204, 439
EcoRII CCWGG 2 cut(s) 411, 418
EcoRV GATATC 1 cut(s) 523
EcoT38I GRGCYC 2 cut(s) 80, 117
FaeI CATG 1 cut(s) 257
FaiI YATR 7 cut(s) 108, 110, 255, 376, 390, 505, 614
FaqI GGGAC 1 cut(s) 429
FatI CATG 1 cut(s) 253
FauI CCCGC 1 cut(s) 452
FbaI TGATCA 1 cut(s) 286
Fnu4HI GCNGC 1 cut(s) 44
FokI GGATG 2 cut(s) 291, 620
FriOI GRGCYC 2 cut(s) 80, 117
Fsp4HI GCNGC 1 cut(s) 44
FspBI CTAG 1 cut(s) 557
GluI GCNGC 1 cut(s) 44
GsuI CTGGAG 1 cut(s) 441
HaeIII GGCC 4 cut(s) 43, 53, 115, 379
Hin1II CATG 1 cut(s) 257
HinfI GANTC 1 cut(s) 365
Hpy166II GTNNAC 2 cut(s) 480, 594
Hpy188I TCNGA 2 cut(s) 210, 358
Hpy188III TCNNGA 7 cut(s) 7, 57, 157, 245, 290, 346, 566
Hpy8I GTNNAC 2 cut(s) 480, 594
HpyAV CCTTC 5 cut(s) 6, 12, 54, 536, 571
HpyCH4IV ACGT 1 cut(s) 617
HpyCH4V TGCA 1 cut(s) 263
HpyF3I CTNAG 2 cut(s) 95, 401
HpySE526I ACGT 1 cut(s) 617
Hsp92II CATG 1 cut(s) 257
Ksp22I TGATCA 1 cut(s) 286
Kzo9I GATC 4 cut(s) 210, 286, 292, 337
LguI GCTCTTC 1 cut(s) 93
LmnI GCTCC 1 cut(s) 83
LpnPI CCDG 6 cut(s) 359, 398, 405, 425, 432, 551
LweI GCATC 1 cut(s) 598
MaeI CTAG 1 cut(s) 557
MaeII ACGT 1 cut(s) 617
MaeIII GTNAC 1 cut(s) 406
MalI GATC 4 cut(s) 212, 288, 294, 339
MbiI CCGCTC 1 cut(s) 162
MboI GATC 4 cut(s) 210, 286, 292, 337
MboII GAAGA 4 cut(s) 80, 160, 371, 374
MflI RGATCY 1 cut(s) 292
MhlI GDGCHC 2 cut(s) 80, 117
MluCI AATT 6 cut(s) 151, 204, 224, 392, 439, 516
MmeI TCCRAC 1 cut(s) 332
MnlI CCTC 7 cut(s) 64, 90, 194, 312, 364, 390, 416
MroXI GAANNNNTTC 1 cut(s) 152
MseI TTAA 1 cut(s) 177
MslI CAYNNNNRTG 1 cut(s) 387
MspA1I CMGCKG 1 cut(s) 94
MspR9I CCNGG 2 cut(s) 413, 420
Mva1269I GAATGC 1 cut(s) 447
MvaI CCWGG 2 cut(s) 413, 420
NdeII GATC 4 cut(s) 210, 286, 292, 337
NlaIII CATG 1 cut(s) 257
NlaIV GGNNCC 3 cut(s) 114, 115, 417
NspV TTCGAA 1 cut(s) 451
PaeR7I CTCGAG 1 cut(s) 55
PasI CCCWGGG 1 cut(s) 412
PciSI GCTCTTC 1 cut(s) 93
PcsI WCGNNNNNNNCGW 1 cut(s) 62
PctI GAATGC 1 cut(s) 447
PdmI GAANNNNTTC 1 cut(s) 152
PfeI GAWTC 1 cut(s) 365
PflMI CCANNNNNTGG 1 cut(s) 227
PkrI GCNGC 1 cut(s) 45
PpuMI RGGWCCY 1 cut(s) 416
Psp124BI GAGCTC 1 cut(s) 80
Psp1406I AACGTT 1 cut(s) 617
Psp5II RGGWCCY 1 cut(s) 416
Psp6I CCWGG 2 cut(s) 411, 418
PspEI GGTNACC 1 cut(s) 406
PspGI CCWGG 2 cut(s) 411, 418
PspN4I GGNNCC 3 cut(s) 114, 115, 417
PspOMI GGGCCC 1 cut(s) 113
PspPI GGNCC 3 cut(s) 113, 114, 416
PspPPI RGGWCCY 1 cut(s) 416
PsuI RGATCY 1 cut(s) 292
RseI CAYNNNNRTG 1 cut(s) 387
SacI GAGCTC 1 cut(s) 80
SapI GCTCTTC 1 cut(s) 93
SaqAI TTAA 1 cut(s) 177
SatI GCNGC 1 cut(s) 44
Sau3AI GATC 4 cut(s) 210, 286, 292, 337
Sau96I GGNCC 3 cut(s) 113, 114, 416
ScrFI CCNGG 2 cut(s) 413, 420
SduI GDGCHC 2 cut(s) 80, 117
SetI ASST 7 cut(s) 23, 65, 80, 101, 275, 421, 620
SfaNI GCATC 1 cut(s) 598
Sfr274I CTCGAG 1 cut(s) 55
SfuI TTCGAA 1 cut(s) 451
SinI GGWCC 1 cut(s) 416
SlaI CTCGAG 1 cut(s) 55
SmiMI CAYNNNNRTG 1 cut(s) 387
SmlI CTYRAG 1 cut(s) 55
SmoI CTYRAG 1 cut(s) 55
Sse9I AATT 6 cut(s) 151, 204, 224, 392, 439, 516
SsiI CCGC 5 cut(s) 44, 92, 162, 445, 589
SspMI CTAG 1 cut(s) 557
SstI GAGCTC 1 cut(s) 80
StyD4I CCNGG 2 cut(s) 411, 418
TaiI ACGT 1 cut(s) 620
TaqI TCGA 2 cut(s) 56, 451
TasI AATT 6 cut(s) 151, 204, 224, 392, 439, 516
TauI GCSGC 1 cut(s) 46
TfiI GAWTC 1 cut(s) 365
Tru1I TTAA 1 cut(s) 177
Tru9I TTAA 1 cut(s) 177
TscAI CASTG 2 cut(s) 469, 532
TspDTI ATGAA 3 cut(s) 113, 152, 405
TspGWI ACGGA 2 cut(s) 70, 185
TspRI CASTG 2 cut(s) 469, 532
Van91I CCANNNNNTGG 1 cut(s) 227
VpaK11BI GGWCC 1 cut(s) 416
XagI CCTNNNNNAGG 1 cut(s) 58
XapI RAATTY 4 cut(s) 151, 204, 392, 439
XhoI CTCGAG 1 cut(s) 55
XmnI GAANNNNTTC 1 cut(s) 152
XspI CTAG 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.