MD09G1216600.v1.1

Bifunctional aspartate aminotransferase and glutamate aspartate-prephenate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007427
Physical Location & Seq
Forward (+)
21532991 .. 21538411
5421 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1216600.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGGGTTCCTATGGACAACTTGCAAGGAGGGCTGTGGAGACTAATATGCCGATCATGGTTCAGATACAGCAGCTGGTCCGAGGAGCCAAAAATGCCGTGTCACTGGCTCAGGGAGTGGTTTATTGGCAACCACCCAAGCAGGCATTGGACAAGGTGAGAGAACTTGTTTGGGAGCCTTCAATTAGTCGTTACGGTGCTGATGAAGGTATACCTGAACTCAGGGAGGCATTGGTACAAAAGTTGCATCGTGAAAATAAGTTGTACAAATCTTCAGTGATGGTTACTGCAGGCGCAAATCAGGCATTTGTGAACCTTGCTCTTACATTGTGTGATGCCGGAGACTCTGTGGTAATGTTTGCACCATACTACTTCAATGCTTACATGTCCTTTCAGATGACAGGAGTCACTAATATACTCGTGGGTCCTGGTCACCCAAAGACACTCTACCCAGATGCAGACTGGTTGGAGAAAACGTTATCCGAAACCAAACCAACCCCAAAACTTGTTACAGTTGTTAATCCTGGCAACCCAAGTGGAACCTATATTCCGGATTCTCTTCTTAAGAGGATATCTGATATATGCAGAGATGCTGGATCCTGGCTAGTTGTGGATAATACATATGAGTATTTTATGTATGATGATTTGAAACACACATGTGTGGAGGGAAATCACATAATCAACATTTTTTCCTTCTCGAAAGCTTATGGGATGATGGGATGGCGTGTTGGATATATAGCTTACCCATCAGAAGTAGAGGGCTTTGGTACCCAACTCCTCAAAGTTCAGGATAACATTCCCATCTGTGCTTCAATAATTTCGCAGCACCTTGCCCTCCACTCATTGGAAATGGGACCCGAATGGGTCACTGAACGAGTGAAAGGTCTAGTCAAGAACAGAGAAATTGTTCTAGAAGCTCTCTCTCCCCTCGGGGACAATGCTGTTAAAGGTGGAGAAGGTGCTATATACTTGTGGGCAAAGCTTCCGGACAAATATGTAGATGATGATAAATTTGTTCACTGGCTTGCTCACAGGCATGGGGTGGTGGTGATCCCCGGAAGTGCTTGTGGATGCCCGGGGAATGTGAGAATCTCCTTCGGTGGCTTGGTGGAGGATGACTGCAAAGCTGCTGCAGAAAGGCTGAGGAGAGGGTTCGAAGAGTTGATCAGAGATGGAATGGTCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000096 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006520 GO:0006521 GO:0006555 GO:0006558 GO:0006568 GO:0006569 GO:0006570 GO:0006576 GO:0006586 GO:0006725 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008483 GO:0009056 GO:0009058 GO:0009063 GO:0009066 GO:0009072 GO:0009074 GO:0009308 GO:0009310 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009641 GO:0009698 GO:0009850 GO:0009851 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010252 GO:0010326 GO:0010364 GO:0010366 GO:0010565 GO:0010600 GO:0010817 GO:0016054 GO:0016740 GO:0016769 GO:0017144 GO:0019222 GO:0019439 GO:0019748 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031335 GO:0031336 GO:0032350 GO:0032351 GO:0032353 GO:0033238 GO:0033239 GO:0034641 GO:0042402 GO:0042430 GO:0042436 GO:0042445 GO:0042446 GO:0042592 GO:0042762 GO:0043436 GO:0044106 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0045763 GO:0046218 GO:0046395 GO:0046483 GO:0046700 GO:0046885 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051175 GO:0062012 GO:0062014 GO:0065007 GO:0065008 GO:0071704 GO:0080090 GO:0090354 GO:0090356 GO:0090357 GO:1900908 GO:1900909 GO:1900911 GO:1900912 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606 GO:1901996 GO:1901997 GO:1902221
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

395

Amino Acids

43.7

Weight (kDa)

5.9

Isoelectric Point (pI)

28.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 33 - 380 4.1e-52 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 764
AccB1I GGYRCC 1 cut(s) 764
AccB7I CCANNNNNTGG 1 cut(s) 841
AccI GTMKAC 1 cut(s) 208
AccIII TCCGGA 2 cut(s) 547, 982
AclI AACGTT 1 cut(s) 473
AclWI GGATC 3 cut(s) 588, 601, 1042
AcsI RAATTY 1 cut(s) 1007
AcuI CTGAAG 1 cut(s) 255
AfaI GTAC 3 cut(s) 234, 263, 766
AfiI CCNNNNNNNGG 1 cut(s) 841
AflII CTTAAG 1 cut(s) 560
AflIII ACRYGT 2 cut(s) 381, 653
AgsI TTSAA 4 cut(s) 180, 373, 646, 810
AjnI CCWGG 3 cut(s) 424, 520, 596
AleI CACNNNNGTG 2 cut(s) 654, 656
AloI GAACNNNNNNTCC 2 cut(s) 529, 561
AluBI AGCT 6 cut(s) 73, 701, 737, 914, 979, 1124
AluI AGCT 6 cut(s) 73, 701, 737, 914, 979, 1124
Alw26I GTCTC 2 cut(s) 32, 333
AlwI GGATC 3 cut(s) 588, 601, 1042
AlwNI CAGNNNCTG 1 cut(s) 73
Ama87I CYCGRG 2 cut(s) 926, 1072
Aor13HI TCCGGA 2 cut(s) 547, 982
ApeKI GCWGC 4 cut(s) 70, 820, 1124, 1127
ApoI RAATTY 1 cut(s) 1007
Asp700I GAANNNNTTC 1 cut(s) 903
Asp718I GGTACC 1 cut(s) 764
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 3 cut(s) 76, 422, 851
AsuC2I CCSGG 3 cut(s) 1053, 1073, 1074
AsuHPI GGTGA 3 cut(s) 166, 422, 1057
AsuII TTCGAA 1 cut(s) 1152
AvaI CYCGRG 2 cut(s) 926, 1072
AvaII GGWCC 3 cut(s) 76, 422, 851
BaeI ACNNNNGTAYC 2 cut(s) 224, 257
BamHI GGATCC 1 cut(s) 593
BanI GGYRCC 1 cut(s) 764
BauI CACGAG 1 cut(s) 416
BbvCI CCTCAGC 1 cut(s) 1139
BbvI GCAGC 4 cut(s) 82, 832, 1111, 1114
BccI CCATC 6 cut(s) 271, 706, 711, 751, 806, 1163
BceAI ACGGC 1 cut(s) 80
BciT130I CCWGG 3 cut(s) 426, 522, 598
BclI TGATCA 1 cut(s) 1161
BcnI CCSGG 3 cut(s) 1053, 1073, 1074
BcoDI GTCTC 2 cut(s) 32, 333
BfaI CTAG 3 cut(s) 602, 884, 908
BfmI CTRYAG 2 cut(s) 285, 1128
BfrI CTTAAG 1 cut(s) 560
BisI GCNGC 4 cut(s) 71, 821, 1125, 1128
BlsI GCNGC 4 cut(s) 72, 822, 1126, 1129
Bme1390I CCNGG 6 cut(s) 426, 522, 598, 1053, 1073, 1074
Bme18I GGWCC 3 cut(s) 76, 422, 851
BmeT110I CYCGRG 2 cut(s) 926, 1072
BmgT120I GGNCC 3 cut(s) 76, 422, 851
BmiI GGNNCC 9 cut(s) 7, 85, 174, 423, 538, 595, 766, 852, 853
BmrFI CCNGG 6 cut(s) 426, 522, 598, 1053, 1073, 1074
BmsI GCATC 5 cut(s) 253, 322, 442, 577, 1058
BoxI GACNNNNGTC 1 cut(s) 401
Bpu10I CCTNAGC 2 cut(s) 108, 1139
Bpu14I TTCGAA 1 cut(s) 1152
BpuMI CCSGG 3 cut(s) 1053, 1073, 1074
BsaJI CCNNGG 5 cut(s) 79, 925, 1051, 1072, 1073
BsaWI WCCGGW 2 cut(s) 547, 982
BsaXI ACNNNNNCTCC 4 cut(s) 330, 360, 458, 488
Bsc4I CCNNNNNNNGG 1 cut(s) 841
Bse1I ACTGG 3 cut(s) 108, 464, 1022
BseAI TCCGGA 2 cut(s) 547, 982
BseBI CCWGG 3 cut(s) 426, 522, 598
BseDI CCNNGG 5 cut(s) 79, 925, 1051, 1072, 1073
BseGI GGATG 4 cut(s) 714, 722, 1073, 1117
BseLI CCNNNNNNNGG 1 cut(s) 841
BseMII CTCAG 3 cut(s) 122, 232, 1130
BseNI ACTGG 3 cut(s) 108, 464, 1022
BseRI GAGGAG 3 cut(s) 96, 764, 1156
BseXI GCAGC 4 cut(s) 82, 832, 1111, 1114
BshNI GGYRCC 1 cut(s) 764
BsiHKCI CYCGRG 2 cut(s) 926, 1072
BsiSI CCGG 5 cut(s) 336, 548, 983, 1053, 1073
BslFI GGGAC 2 cut(s) 864, 944
BslI CCNNNNNNNGG 1 cut(s) 841
BsmAI GTCTC 2 cut(s) 32, 333
BsmFI GGGAC 2 cut(s) 864, 944
BsoBI CYCGRG 2 cut(s) 926, 1072
Bsp119I TTCGAA 1 cut(s) 1152
Bsp13I TCCGGA 2 cut(s) 547, 982
Bsp1407I TGTACA 1 cut(s) 261
Bsp143I GATC 4 cut(s) 51, 593, 1047, 1161
BspCNI CTCAG 3 cut(s) 121, 231, 1131
BspEI TCCGGA 2 cut(s) 547, 982
BspLI GGNNCC 9 cut(s) 7, 85, 174, 423, 538, 595, 766, 852, 853
BspMAI CTGCAG 2 cut(s) 289, 1132
BspPI GGATC 3 cut(s) 588, 601, 1042
BspT104I TTCGAA 1 cut(s) 1152
BspT107I GGYRCC 1 cut(s) 764
BspTI CTTAAG 1 cut(s) 560
BsrGI TGTACA 1 cut(s) 261
BsrI ACTGG 3 cut(s) 108, 464, 1022
BssECI CCNNGG 5 cut(s) 79, 925, 1051, 1072, 1073
BssMI GATC 4 cut(s) 51, 593, 1047, 1161
BssNAI GTATAC 1 cut(s) 209
BssSI CACGAG 1 cut(s) 416
Bst1107I GTATAC 1 cut(s) 209
Bst2BI CACGAG 1 cut(s) 416
Bst2UI CCWGG 3 cut(s) 426, 522, 598
Bst4CI ACNGT 2 cut(s) 194, 511
Bst6I CTCTTC 2 cut(s) 561, 1149
BstAFI CTTAAG 1 cut(s) 560
BstAUI TGTACA 1 cut(s) 261
BstBI TTCGAA 1 cut(s) 1152
BstC8I GCNNGC 3 cut(s) 141, 289, 1023
BstDEI CTNAG 3 cut(s) 108, 218, 1139
BstEII GGTNACC 1 cut(s) 428
BstF5I GGATG 4 cut(s) 714, 722, 1073, 1117
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 4 cut(s) 54, 596, 1050, 1164
BstMAI GTCTC 2 cut(s) 32, 333
BstMBI GATC 4 cut(s) 51, 593, 1047, 1161
BstMWI GCNNNNNNNGC 3 cut(s) 29, 92, 299
BstNI CCWGG 3 cut(s) 426, 522, 598
BstNSI RCATGY 2 cut(s) 385, 657
BstPAI GACNNNNGTC 1 cut(s) 401
BstPI GGTNACC 1 cut(s) 428
BstSCI CCNGG 6 cut(s) 424, 520, 596, 1051, 1071, 1072
BstSFI CTRYAG 2 cut(s) 285, 1128
BstV1I GCAGC 4 cut(s) 82, 832, 1111, 1114
BstX2I RGATCY 1 cut(s) 593
BstYI RGATCY 1 cut(s) 593
BstZ17I GTATAC 1 cut(s) 209
BtsCI GGATG 4 cut(s) 714, 722, 1073, 1117
BtsIMutI CAGTG 4 cut(s) 101, 279, 864, 1015
Cac8I GCNNGC 3 cut(s) 141, 289, 1023
CaiI CAGNNNCTG 1 cut(s) 73
CfoI GCGC 1 cut(s) 293
Cfr13I GGNCC 3 cut(s) 76, 422, 851
Cfr9I CCCGGG 1 cut(s) 1072
Csp6I GTAC 3 cut(s) 233, 262, 765
CspCI CAANNNNNGTGG 2 cut(s) 514, 549
CviAII CATG 4 cut(s) 55, 382, 654, 1034
CviQI GTAC 3 cut(s) 233, 262, 765
DdeI CTNAG 3 cut(s) 108, 218, 1139
DpnI GATC 4 cut(s) 53, 595, 1049, 1163
DpnII GATC 4 cut(s) 51, 593, 1047, 1161
Eam1104I CTCTTC 2 cut(s) 561, 1149
EarI CTCTTC 2 cut(s) 561, 1149
Eco32I GATATC 1 cut(s) 570
Eco47I GGWCC 3 cut(s) 76, 422, 851
Eco57I CTGAAG 1 cut(s) 255
Eco88I CYCGRG 2 cut(s) 926, 1072
Eco91I GGTNACC 1 cut(s) 428
EcoO109I RGGNCCY 2 cut(s) 422, 851
EcoO65I GGTNACC 1 cut(s) 428
EcoRII CCWGG 3 cut(s) 424, 520, 596
EcoRV GATATC 1 cut(s) 570
FaeI CATG 4 cut(s) 58, 385, 657, 1037
FaqI GGGAC 2 cut(s) 864, 944
FatI CATG 4 cut(s) 54, 381, 653, 1033
FauNDI CATATG 1 cut(s) 619
FbaI TGATCA 1 cut(s) 1161
FblI GTMKAC 1 cut(s) 208
Fnu4HI GCNGC 4 cut(s) 71, 821, 1125, 1128
FokI GGATG 4 cut(s) 721, 729, 1080, 1124
Fsp4HI GCNGC 4 cut(s) 71, 821, 1125, 1128
FspBI CTAG 3 cut(s) 602, 884, 908
GlaI GCGC 1 cut(s) 292
GluI GCNGC 4 cut(s) 71, 821, 1125, 1128
HapII CCGG 5 cut(s) 336, 548, 983, 1053, 1073
HhaI GCGC 1 cut(s) 293
Hin1II CATG 4 cut(s) 58, 385, 657, 1037
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HindIII AAGCTT 2 cut(s) 699, 977
HinfI GANTC 4 cut(s) 341, 402, 551, 1086
HpaII CCGG 5 cut(s) 336, 548, 983, 1053, 1073
HphI GGTGA 3 cut(s) 166, 422, 1057
Hpy166II GTNNAC 3 cut(s) 209, 310, 1015
Hpy188I TCNGA 7 cut(s) 63, 80, 393, 481, 574, 748, 1166
Hpy188III TCNNGA 7 cut(s) 248, 548, 694, 785, 889, 908, 983
Hpy8I GTNNAC 3 cut(s) 209, 310, 1015
HpyAV CCTTC 5 cut(s) 186, 197, 700, 947, 1102
HpyCH4III ACNGT 2 cut(s) 194, 511
HpyCH4IV ACGT 1 cut(s) 473
HpyCH4V TGCA 8 cut(s) 23, 244, 287, 359, 455, 582, 1119, 1130
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 92, 299
HpyF3I CTNAG 3 cut(s) 108, 218, 1139
HpySE526I ACGT 1 cut(s) 473
Hsp92II CATG 4 cut(s) 58, 385, 657, 1037
HspAI GCGC 1 cut(s) 291
KflI GGGWCCC 1 cut(s) 851
Kpn2I TCCGGA 2 cut(s) 547, 982
KpnI GGTACC 1 cut(s) 768
Ksp22I TGATCA 1 cut(s) 1161
Kzo9I GATC 4 cut(s) 51, 593, 1047, 1161
LmnI GCTCC 2 cut(s) 83, 172
Lsp1109I GCAGC 4 cut(s) 82, 832, 1111, 1114
LweI GCATC 5 cut(s) 253, 322, 442, 577, 1058
MaeI CTAG 3 cut(s) 602, 884, 908
MaeII ACGT 1 cut(s) 473
MaeIII GTNAC 7 cut(s) 99, 188, 280, 403, 428, 505, 862
MalI GATC 4 cut(s) 53, 595, 1049, 1163
MboI GATC 4 cut(s) 51, 593, 1047, 1161
MboII GAAGA 3 cut(s) 261, 548, 1166
MflI RGATCY 1 cut(s) 593
MluCI AATT 4 cut(s) 180, 813, 900, 1007
MlyI GAGTC 2 cut(s) 335, 411
MmeI TCCRAC 2 cut(s) 444, 706
MroI TCCGGA 2 cut(s) 547, 982
MroXI GAANNNNTTC 1 cut(s) 903
MseI TTAA 3 cut(s) 516, 561, 942
MslI CAYNNNNRTG 3 cut(s) 654, 656, 1032
MspA1I CMGCKG 1 cut(s) 73
MspCI CTTAAG 1 cut(s) 560
MspI CCGG 5 cut(s) 336, 548, 983, 1053, 1073
MspR9I CCNGG 6 cut(s) 426, 522, 598, 1053, 1073, 1074
MvaI CCWGG 3 cut(s) 426, 522, 598
MwoI GCNNNNNNNGC 3 cut(s) 29, 92, 299
NciI CCSGG 3 cut(s) 1053, 1073, 1074
NdeI CATATG 1 cut(s) 619
NdeII GATC 4 cut(s) 51, 593, 1047, 1161
NlaIII CATG 4 cut(s) 58, 385, 657, 1037
NlaIV GGNNCC 9 cut(s) 7, 85, 174, 423, 538, 595, 766, 852, 853
NmuCI GTSAC 4 cut(s) 99, 403, 428, 862
NspI RCATGY 2 cut(s) 385, 657
NspV TTCGAA 1 cut(s) 1152
OliI CACNNNNGTG 2 cut(s) 654, 656
PciI ACATGT 2 cut(s) 381, 653
PdmI GAANNNNTTC 1 cut(s) 903
PfeI GAWTC 2 cut(s) 551, 1086
PflMI CCANNNNNTGG 1 cut(s) 841
PkrI GCNGC 4 cut(s) 72, 822, 1126, 1129
PleI GAGTC 2 cut(s) 335, 410
PpsI GAGTC 2 cut(s) 335, 410
PpuMI RGGWCCY 2 cut(s) 422, 851
PscI ACATGT 2 cut(s) 381, 653
PshAI GACNNNNGTC 1 cut(s) 401
Psp1406I AACGTT 1 cut(s) 473
Psp5II RGGWCCY 2 cut(s) 422, 851
Psp6I CCWGG 3 cut(s) 424, 520, 596
PspEI GGTNACC 1 cut(s) 428
PspGI CCWGG 3 cut(s) 424, 520, 596
PspN4I GGNNCC 9 cut(s) 7, 85, 174, 423, 538, 595, 766, 852, 853
PspPI GGNCC 3 cut(s) 76, 422, 851
PspPPI RGGWCCY 2 cut(s) 422, 851
PstI CTGCAG 2 cut(s) 289, 1132
PstNI CAGNNNCTG 1 cut(s) 73
PsuI RGATCY 1 cut(s) 593
PvuII CAGCTG 1 cut(s) 73
RsaI GTAC 3 cut(s) 234, 263, 766
RsaNI GTAC 3 cut(s) 233, 262, 765
RseI CAYNNNNRTG 3 cut(s) 654, 656, 1032
SaqAI TTAA 3 cut(s) 516, 561, 942
SatI GCNGC 4 cut(s) 71, 821, 1125, 1128
Sau3AI GATC 4 cut(s) 51, 593, 1047, 1161
Sau96I GGNCC 3 cut(s) 76, 422, 851
SchI GAGTC 2 cut(s) 335, 411
ScrFI CCNGG 6 cut(s) 426, 522, 598, 1053, 1073, 1074
SfaNI GCATC 5 cut(s) 253, 322, 442, 577, 1058
SfcI CTRYAG 2 cut(s) 285, 1128
SfuI TTCGAA 1 cut(s) 1152
SinI GGWCC 3 cut(s) 76, 422, 851
SmaI CCCGGG 1 cut(s) 1074
SmiMI CAYNNNNRTG 3 cut(s) 654, 656, 1032
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
Sse9I AATT 4 cut(s) 180, 813, 900, 1007
SspMI CTAG 3 cut(s) 602, 884, 908
StyD4I CCNGG 6 cut(s) 424, 520, 596, 1051, 1071, 1072
TaaI ACNGT 2 cut(s) 194, 511
TaiI ACGT 1 cut(s) 476
TaqI TCGA 3 cut(s) 695, 1152, 1179
TasI AATT 4 cut(s) 180, 813, 900, 1007
TatI WGTACW 1 cut(s) 261
TfiI GAWTC 2 cut(s) 551, 1086
Tru1I TTAA 3 cut(s) 516, 561, 942
Tru9I TTAA 3 cut(s) 516, 561, 942
TscAI CASTG 4 cut(s) 108, 279, 871, 1022
TseFI GTSAC 4 cut(s) 99, 403, 428, 862
TseI GCWGC 4 cut(s) 70, 820, 1124, 1127
Tsp45I GTSAC 4 cut(s) 99, 403, 428, 862
TspDTI ATGAA 1 cut(s) 216
TspMI CCCGGG 1 cut(s) 1072
TspRI CASTG 4 cut(s) 108, 279, 871, 1022
Van91I CCANNNNNTGG 1 cut(s) 841
Vha464I CTTAAG 1 cut(s) 560
VpaK11BI GGWCC 3 cut(s) 76, 422, 851
XapI RAATTY 1 cut(s) 1007
XbaI TCTAGA 1 cut(s) 907
XceI RCATGY 2 cut(s) 385, 657
XcmI CCANNNNNNNNNTGG 2 cut(s) 142, 456
XmaI CCCGGG 1 cut(s) 1072
XmiI GTMKAC 1 cut(s) 208
XmnI GAANNNNTTC 1 cut(s) 903
XspI CTAG 3 cut(s) 602, 884, 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.