Rorug02G0340300

Bifunctional aspartate aminotransferase and glutamate aspartate-prephenate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007427
Physical Location & Seq
Forward (+)
42343204 .. 42345375
2172 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0340300.1

Sequence Viewer

Length: 2172 bp
ATGAGCGCCGCCGCCGCTACGATCACAGACCTGCCGGAGGCGATTCTCGCCGCCATCATCGGCTTGGTCTCCGACACCCGCTCCCGCAACTCAGTCGCCCTCGTCTGCCGCAAATTCAGCAGCCTCGAGAGAGCCACGCGCACCTCCCTCACGCTCCGCGGCAACGCGCGGGACCTCCACCTGATCCCGACCTGCTTCACCTCCGTCTCCGACCTCGACCTCTCCCTCCTCTCGCCGTGGGGCCACGCGCTGCTCTCCCCCTCCGCCTCCTCCACCGACCCCGCCCTCCTCGCCCAGCGCCTCCGCGTCGCCTTCCCCTCCGTCACCTCCCTCACCGTCTACTCCCGATCCTCCTCCACCGTCCAGATTGTCGCCAATCTCTGGCCGGGACTCCGCCGCGTCAAGCTCGTCCGATGGCATCTACGGCCGCAATCGCTTCTCGGAGCCGACTTCGATCCTCTCTTCCAGCAATGCCAGACTCTCTCCGATTTGGACTTGTCGGAATTCTACTACTGGACCGATGACCTCCCTCCGGTTCTGGAAGCCCATCCCAACGTGGCCCGCTCTCTCACCAAGCTCAATCTCCTGACGGCGTCGTTTACCGAGGGTTTCAAAGCCAACGAGATCCGATCCATCACCGAGGCCTGCCCCAAGCTTCAGCAGCTCTTCGTCGCTTGTATGTTCGATCCCAGATACATAGGGTTCGTCGGGGAGGAGGCTCTCTTGGCCGTATCGACCAATTGTCCGGAGCTCAGAGTCGTCCATCTCATCGATACGTCGTCGTTGGCCAACGCTCGTGGGGATCCAAACGACGACGGTTTCACATCGGAGGACGCTCAGATTGGGCGAGCGGCTTTGATCGACTTCTTCTCCGGGCTTCCTTTGCTGGAAGAGCTGGTGCTCGATGTGTGCAAGAACGTGAGGGACAGTGGACTGGCCTTGGAGGTTCTTGCTTCCAAGTGTCCCAGACTGAGAGCTCTTAAGGTTGGGCAGTTCCATGGGCTGTGCTCGGCGGTTGGGTCCCAGCTCGACGGCATTGCTCTATGTCAAGGGCTGGAGTCACTGTCGATTAAGAACTCTGCAGATTTGACAGATATGGGATTGATCGAAATCGCGAGAGGGTGTTGTAAGCTTGCCAAGTTTGAGGTGTATGGATGCAAGAAAATTACAGTGAAGGGGTTGAGGACGCTGGCTTGTTTGTTGAGGAAGACTTTAGTTGATGTGAGCATATCTTGCTGTAAGAACCTGGATGCTGCAGGCTCAGTGTGTGCTCTGGAGCCAATTCGCGATCGGATTCAACGTCTGCACATAGATTGTGTCTGGCCACGGGACTTTGATCTCAATCAAGTCAATGATTATGATGTTGGTGATGATGAAAGTGTTGTGATGAGTCGGAATGGTAATGATGACATGGACCTGAAATGGAGTGATGATTACGAGCACAGGATCAGCAAGAAGTGCAAGTATGGATCAGATGGGGACTGTTCGTATATGCCAACCAATGGCTACGCGAATGGAAATGGCCATGGTAATGGAAATGGGTTTTGGAGTGATGAGACTTGGGAAAGGCTGCACTACCTATCACTTTGGATTGAGGTGGGGGAGTTGCTGACATCATTGCCAGAGGTGGGTCTGGACGAGTGCCCCAATCTGGAGGAGATTCAGATCCGGGTCGAGGGCGATTGCAGGGGAAGGCACAAGCCCACACAGCGAGAATTCGGGTTGAGCTGCCTATCCCGTTTTCCTCGGCTGTCAAAGATGAAGCTGGATTGTGGTGATACTGTGGGATATGCACTGACTGCTCCACCAGGACAGATGGACTTGTCACTTTGGGAGAGGTTCTTCCTCTCTGGGATTGGAGACTTGAGTCTCAGCGAAGTCGATTATTGGCCACCTCAAGACCGGGATGTGAACCAGAGGAGTCTCTGGCTTCCAGCTGCCGGATTGCTGGCAGAATGCATCACTCTCCGGAAGCTTTTCATCCATGGTACTGCTCACGAACATTTCATGATGTTCCTTGTGAGAAACCCAAACCTCAACCTCAGCTTAAGGGATGTGCAACTGAGAGGGGATTACTATCCAGCGCCTGAAAATGAAATGAGTACAGAGATGAGGGTGGACTCGTGCTGTCGCTTTGAAGATGCGCTGAACAGGCGCCTAATCCTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000096 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006520 GO:0006521 GO:0006555 GO:0006558 GO:0006568 GO:0006569 GO:0006570 GO:0006576 GO:0006586 GO:0006725 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008483 GO:0009056 GO:0009058 GO:0009063 GO:0009066 GO:0009072 GO:0009074 GO:0009308 GO:0009310 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009641 GO:0009698 GO:0009850 GO:0009851 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010252 GO:0010326 GO:0010364 GO:0010366 GO:0010565 GO:0010600 GO:0010817 GO:0016054 GO:0016740 GO:0016769 GO:0017144 GO:0019222 GO:0019439 GO:0019748 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031335 GO:0031336 GO:0032350 GO:0032351 GO:0032353 GO:0033238 GO:0033239 GO:0034641 GO:0042402 GO:0042430 GO:0042436 GO:0042445 GO:0042446 GO:0042592 GO:0042762 GO:0043436 GO:0044106 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0045763 GO:0046218 GO:0046395 GO:0046483 GO:0046700 GO:0046885 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051175 GO:0062012 GO:0062014 GO:0065007 GO:0065008 GO:0071704 GO:0080090 GO:0090354 GO:0090356 GO:0090357 GO:1900908 GO:1900909 GO:1900911 GO:1900912 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606 GO:1901996 GO:1901997 GO:1902221
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

723

Amino Acids

80.29

Weight (kDa)

5.27

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box_5 PF18511 10 - 48 7.7e-07 F-box
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 39, 200
AccB1I GGYRCC 1 cut(s) 2154
AccB7I CCANNNNNTGG 2 cut(s) 381, 1502
AccBSI CCGCTC 3 cut(s) 81, 564, 851
AccI GTMKAC 1 cut(s) 339
AccIII TCCGGA 2 cut(s) 745, 1968
AcoI YGGCCR 7 cut(s) 383, 425, 726, 786, 1322, 1522, 1889
AcsI RAATTY 3 cut(s) 113, 503, 1715
AcuI CTGAAG 1 cut(s) 641
AcyI GRCGYC 2 cut(s) 593, 2155
AfaI GTAC 2 cut(s) 1990, 2104
AfiI CCNNNNNNNGG 8 cut(s) 37, 381, 532, 1502, 1628, 1651, 1675, 1940
AflII CTTAAG 2 cut(s) 980, 2047
AgsI TTSAA 3 cut(s) 613, 1298, 2138
AhdI GACNNNNNGTC 1 cut(s) 741
AjnI CCWGG 2 cut(s) 1245, 1807
AjuI GAANNNNNNNTTGG 2 cut(s) 1528, 1560
Alw21I GWGCWC 6 cut(s) 753, 903, 979, 1010, 1273, 1443
Alw26I GTCTC 6 cut(s) 73, 211, 1550, 1854, 1874, 1928
AlwNI CAGNNNCTG 1 cut(s) 2087
Ama87I CYCGRG 1 cut(s) 125
Aor13HI TCCGGA 2 cut(s) 745, 1968
ApeKI GCWGC 7 cut(s) 120, 250, 661, 1253, 1570, 1728, 1937
ApoI RAATTY 3 cut(s) 113, 503, 1715
Asp700I GAANNNNTTC 1 cut(s) 1976
AspLEI GCGC 8 cut(s) 8, 141, 169, 250, 300, 2086, 2146, 2157
AspS9I GGNCC 6 cut(s) 172, 241, 516, 559, 1020, 1414
AsuC2I CCSGG 4 cut(s) 387, 874, 1670, 1904
AsuHPI GGTGA 7 cut(s) 190, 316, 325, 562, 628, 1379, 1787
AvaI CYCGRG 1 cut(s) 125
AvaII GGWCC 4 cut(s) 172, 516, 1020, 1414
BaeGI GKGCMC 1 cut(s) 1646
BalI TGGCCA 4 cut(s) 788, 1324, 1524, 1891
BamHI GGATCC 1 cut(s) 802
BanI GGYRCC 1 cut(s) 2154
BanII GRGCYC 2 cut(s) 753, 979
BauI CACGAG 2 cut(s) 795, 2122
BbsI GAAGAC 1 cut(s) 1214
Bbv12I GWGCWC 6 cut(s) 753, 903, 979, 1010, 1273, 1443
BbvCI CCTCAGC 1 cut(s) 2042
BbvI GCAGC 7 cut(s) 132, 237, 673, 1240, 1557, 1715, 1924
BccI CCATC 7 cut(s) 62, 408, 555, 641, 771, 1469, 1810
BceAI ACGGC 5 cut(s) 220, 440, 606, 713, 1048
BcgI CGANNNNNNTGC 4 cut(s) 76, 110, 1019, 1053
BciT130I CCWGG 2 cut(s) 1247, 1809
BcnI CCSGG 4 cut(s) 387, 874, 1670, 1904
BcoDI GTCTC 6 cut(s) 73, 211, 1550, 1854, 1874, 1928
BfmI CTRYAG 2 cut(s) 1080, 1254
BfoI RGCGCY 4 cut(s) 9, 301, 2087, 2158
BfrI CTTAAG 2 cut(s) 980, 2047
BfuAI ACCTGC 2 cut(s) 39, 200
Bme1390I CCNGG 6 cut(s) 387, 874, 1247, 1670, 1809, 1904
Bme18I GGWCC 4 cut(s) 172, 516, 1020, 1414
BmeRI GACNNNNNGTC 1 cut(s) 741
BmeT110I CYCGRG 1 cut(s) 125
BmgT120I GGNCC 6 cut(s) 172, 241, 516, 559, 1020, 1414
BmiI GGNNCC 8 cut(s) 173, 242, 445, 804, 1021, 1022, 1278, 2156
BmrFI CCNGG 6 cut(s) 387, 874, 1247, 1670, 1809, 1904
BmsI GCATC 5 cut(s) 427, 1145, 1240, 1968, 2131
BoxI GACNNNNGTC 1 cut(s) 1866
BpiI GAAGAC 1 cut(s) 1214
BpmI CTGGAG 3 cut(s) 1076, 1295, 1673
Bpu10I CCTNAGC 1 cut(s) 2042
BpuEI CTTGAG 2 cut(s) 1881, 1885
BpuMI CCSGG 4 cut(s) 387, 874, 1670, 1904
Bsa29I ATCGAT 1 cut(s) 771
BsaBI GATNNNNATC 4 cut(s) 1109, 1341, 1664, 2076
BsaHI GRCGYC 2 cut(s) 593, 2155
BsaI GGTCTC 1 cut(s) 73
BsaWI WCCGGW 3 cut(s) 532, 745, 1968
BsaXI ACNNNNNCTCC 6 cut(s) 65, 95, 854, 884, 1049, 1079
Bsc4I CCNNNNNNNGG 8 cut(s) 37, 381, 532, 1502, 1628, 1651, 1675, 1940
Bse1I ACTGG 2 cut(s) 518, 939
Bse3DI GCAATG 3 cut(s) 476, 1035, 1616
Bse8I GATNNNNATC 4 cut(s) 1109, 1341, 1664, 2076
BseAI TCCGGA 2 cut(s) 745, 1968
BseBI CCWGG 2 cut(s) 1247, 1809
BseCI ATCGAT 1 cut(s) 771
BseGI GGATG 6 cut(s) 547, 1160, 1255, 1912, 1980, 2059
BseJI GATNNNNATC 4 cut(s) 1109, 1341, 1664, 2076
BseLI CCNNNNNNNGG 8 cut(s) 37, 381, 532, 1502, 1628, 1651, 1675, 1940
BseMI GCAATG 3 cut(s) 476, 1035, 1616
BseMII CTCAG 8 cut(s) 105, 766, 851, 962, 1275, 1885, 2054, 2056
BseNI ACTGG 2 cut(s) 518, 939
BseRI GAGGAG 7 cut(s) 218, 259, 278, 343, 728, 1670, 1933
BseSI GKGCMC 1 cut(s) 1646
BseX3I CGGCCG 1 cut(s) 425
BseXI GCAGC 7 cut(s) 132, 237, 673, 1240, 1557, 1715, 1924
BseYI CCCAGC 2 cut(s) 294, 1023
BsgI GTGCAG 2 cut(s) 1289, 1556
Bsh1285I CGRYCG 2 cut(s) 428, 1291
BshNI GGYRCC 1 cut(s) 2154
BshVI ATCGAT 1 cut(s) 771
BsiEI CGRYCG 2 cut(s) 428, 1291
BsiHKAI GWGCWC 6 cut(s) 753, 903, 979, 1010, 1273, 1443
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 9 cut(s) 35, 386, 533, 746, 873, 1669, 1903, 1941, 1969
BslFI GGGAC 7 cut(s) 185, 402, 938, 948, 1006, 1343, 1493
BslI CCNNNNNNNGG 8 cut(s) 37, 381, 532, 1502, 1628, 1651, 1675, 1940
BsmAI GTCTC 6 cut(s) 73, 211, 1550, 1854, 1874, 1928
BsmBI CGTCTC 1 cut(s) 211
BsmFI GGGAC 7 cut(s) 185, 402, 938, 948, 1006, 1343, 1493
BsmI GAATGC 1 cut(s) 1961
Bso31I GGTCTC 1 cut(s) 73
BsoBI CYCGRG 1 cut(s) 125
Bsp1286I GDGCHC 7 cut(s) 753, 903, 979, 1010, 1273, 1443, 1646
Bsp13I TCCGGA 2 cut(s) 745, 1968
Bsp19I CCATGG 3 cut(s) 997, 1525, 1984
Bsp68I TCGCGA 2 cut(s) 1115, 1287
BspCNI CTCAG 8 cut(s) 104, 765, 850, 963, 1274, 1884, 2055, 2055
BspDI ATCGAT 1 cut(s) 771
BspEI TCCGGA 2 cut(s) 745, 1968
BspHI TCATGA 1 cut(s) 2007
BspLI GGNNCC 8 cut(s) 173, 242, 445, 804, 1021, 1022, 1278, 2156
BspMAI CTGCAG 2 cut(s) 1084, 1258
BspMI ACCTGC 2 cut(s) 39, 200
BspQI GCTCTTC 2 cut(s) 671, 885
BspT107I GGYRCC 1 cut(s) 2154
BspTI CTTAAG 2 cut(s) 980, 2047
BspTNI GGTCTC 1 cut(s) 73
BsrBI CCGCTC 3 cut(s) 81, 564, 851
BsrDI GCAATG 3 cut(s) 476, 1035, 1616
BsrI ACTGG 2 cut(s) 518, 939
BssNI GRCGYC 2 cut(s) 593, 2155
BssSI CACGAG 2 cut(s) 795, 2122
BssT1I CCWWGG 4 cut(s) 939, 997, 1525, 1984
Bst2BI CACGAG 2 cut(s) 795, 2122
Bst2UI CCWGG 2 cut(s) 1247, 1809
Bst4CI ACNGT 8 cut(s) 337, 361, 818, 929, 1065, 1171, 1484, 1783
Bst6I CTCTTC 3 cut(s) 467, 671, 885
BstACI GRCGYC 2 cut(s) 593, 2155
BstAFI CTTAAG 2 cut(s) 980, 2047
BstAPI GCANNNNNTGC 3 cut(s) 1233, 1458, 1799
BstC8I GCNNGC 7 cut(s) 562, 646, 849, 1134, 1191, 1258, 1950
BstDEI CTNAG 8 cut(s) 91, 752, 837, 971, 1261, 1871, 2042, 2063
BstDSI CCRYGG 6 cut(s) 157, 236, 997, 1325, 1525, 1984
BstENI CCTNNNNNAGG 1 cut(s) 35
BstF5I GGATG 6 cut(s) 547, 1160, 1255, 1912, 1980, 2059
BstH2I RGCGCY 4 cut(s) 9, 301, 2087, 2158
BstHHI GCGC 8 cut(s) 8, 141, 169, 250, 300, 2086, 2146, 2157
BstMAI GTCTC 6 cut(s) 73, 211, 1550, 1854, 1874, 1928
BstMCI CGRYCG 2 cut(s) 428, 1291
BstNI CCWGG 2 cut(s) 1247, 1809
BstPAI GACNNNNGTC 1 cut(s) 1866
BstSCI CCNGG 6 cut(s) 385, 872, 1245, 1668, 1807, 1902
BstSFI CTRYAG 2 cut(s) 1080, 1254
BstSLI GKGCMC 1 cut(s) 1646
BstV1I GCAGC 7 cut(s) 132, 237, 673, 1240, 1557, 1715, 1924
BstV2I GAAGAC 1 cut(s) 1214
BstX2I RGATCY 3 cut(s) 624, 802, 1665
BstXI CCANNNNNNTGG 1 cut(s) 1532
BstYI RGATCY 3 cut(s) 624, 802, 1665
BstZI CGGCCG 1 cut(s) 425
Bsu15I ATCGAT 1 cut(s) 771
BsuTUI ATCGAT 1 cut(s) 771
BtgI CCRYGG 6 cut(s) 157, 236, 997, 1325, 1525, 1984
BtsCI GGATG 6 cut(s) 547, 1160, 1255, 1912, 1980, 2059
BtsIMutI CAGTG 5 cut(s) 934, 1061, 1176, 1269, 1793
BtuMI TCGCGA 2 cut(s) 1115, 1287
BveI ACCTGC 2 cut(s) 39, 200
Cac8I GCNNGC 7 cut(s) 562, 646, 849, 1134, 1191, 1258, 1950
CaiI CAGNNNCTG 1 cut(s) 2087
CciI TCATGA 1 cut(s) 2007
CfoI GCGC 8 cut(s) 8, 141, 169, 250, 300, 2086, 2146, 2157
Cfr13I GGNCC 6 cut(s) 172, 241, 516, 559, 1020, 1414
Cfr42I CCGCGG 1 cut(s) 160
ClaI ATCGAT 1 cut(s) 771
CseI GACGC 5 cut(s) 295, 388, 582, 842, 1195
Csp6I GTAC 2 cut(s) 1989, 2103
CspCI CAANNNNNGTGG 2 cut(s) 778, 813
CviAII CATG 5 cut(s) 998, 1411, 1526, 1985, 2008
CviQI GTAC 2 cut(s) 1989, 2103
DdeI CTNAG 8 cut(s) 91, 752, 837, 971, 1261, 1871, 2042, 2063
DinI GGCGCC 1 cut(s) 2156
DriI GACNNNNNGTC 1 cut(s) 741
EaeI YGGCCR 7 cut(s) 383, 425, 726, 786, 1322, 1522, 1889
EagI CGGCCG 1 cut(s) 425
Eam1104I CTCTTC 3 cut(s) 467, 671, 885
Eam1105I GACNNNNNGTC 1 cut(s) 741
EarI CTCTTC 3 cut(s) 467, 671, 885
EciI GGCGGA 2 cut(s) 253, 383
Ecl136II GAGCTC 2 cut(s) 751, 977
EclXI CGGCCG 1 cut(s) 425
Eco130I CCWWGG 4 cut(s) 939, 997, 1525, 1984
Eco147I AGGCCT 1 cut(s) 644
Eco24I GRGCYC 2 cut(s) 753, 979
Eco31I GGTCTC 1 cut(s) 73
Eco47I GGWCC 4 cut(s) 172, 516, 1020, 1414
Eco52I CGGCCG 1 cut(s) 425
Eco53kI GAGCTC 2 cut(s) 751, 977
Eco57I CTGAAG 1 cut(s) 641
Eco88I CYCGRG 1 cut(s) 125
EcoICRI GAGCTC 2 cut(s) 751, 977
EcoNI CCTNNNNNAGG 1 cut(s) 35
EcoO109I RGGNCCY 2 cut(s) 172, 1020
EcoRI GAATTC 2 cut(s) 503, 1715
EcoRII CCWGG 2 cut(s) 1245, 1807
EcoT14I CCWWGG 4 cut(s) 939, 997, 1525, 1984
EcoT22I ATGCAT 1 cut(s) 1961
EcoT38I GRGCYC 2 cut(s) 753, 979
EgeI GGCGCC 1 cut(s) 2156
EheI GGCGCC 1 cut(s) 2156
ErhI CCWWGG 4 cut(s) 939, 997, 1525, 1984
Esp3I CGTCTC 1 cut(s) 211
FaeI CATG 5 cut(s) 1001, 1414, 1529, 1988, 2011
FaqI GGGAC 7 cut(s) 185, 402, 938, 948, 1006, 1343, 1493
FatI CATG 5 cut(s) 997, 1410, 1525, 1984, 2007
FauI CCCGC 5 cut(s) 86, 92, 162, 289, 569
FblI GTMKAC 1 cut(s) 339
FokI GGATG 6 cut(s) 534, 1167, 1262, 1919, 1967, 2066
FriOI GRGCYC 2 cut(s) 753, 979
GlaI GCGC 8 cut(s) 7, 140, 168, 249, 299, 2085, 2145, 2156
GsaI CCCAGC 2 cut(s) 298, 1027
GsuI CTGGAG 3 cut(s) 1076, 1295, 1673
HaeII RGCGCY 4 cut(s) 9, 301, 2087, 2158
HapII CCGG 9 cut(s) 35, 386, 533, 746, 873, 1669, 1903, 1941, 1969
HgaI GACGC 5 cut(s) 295, 388, 582, 842, 1195
HhaI GCGC 8 cut(s) 8, 141, 169, 250, 300, 2086, 2146, 2157
Hin1I GRCGYC 2 cut(s) 593, 2155
Hin1II CATG 5 cut(s) 1001, 1414, 1529, 1988, 2011
Hin6I GCGC 8 cut(s) 6, 139, 167, 248, 298, 2084, 2144, 2155
HinP1I GCGC 8 cut(s) 6, 139, 167, 248, 298, 2084, 2144, 2155
HindIII AAGCTT 3 cut(s) 653, 1130, 1973
HpaII CCGG 9 cut(s) 35, 386, 533, 746, 873, 1669, 1903, 1941, 1969
HphI GGTGA 7 cut(s) 190, 316, 325, 562, 628, 1379, 1787
Hpy166II GTNNAC 5 cut(s) 340, 600, 932, 1912, 2119
Hpy8I GTNNAC 5 cut(s) 340, 600, 932, 1912, 2119
Hpy99I CGWCG 9 cut(s) 311, 598, 674, 710, 781, 784, 815, 818, 1034
HpyAV CCTTC 3 cut(s) 322, 1168, 1686
HpyCH4III ACNGT 8 cut(s) 337, 361, 818, 929, 1065, 1171, 1484, 1783
HpyCH4IV ACGT 4 cut(s) 555, 776, 918, 1300
HpyF3I CTNAG 8 cut(s) 91, 752, 837, 971, 1261, 1871, 2042, 2063
HpySE526I ACGT 4 cut(s) 555, 776, 918, 1300
Hsp92I GRCGYC 2 cut(s) 593, 2155
Hsp92II CATG 5 cut(s) 1001, 1414, 1529, 1988, 2011
HspAI GCGC 8 cut(s) 6, 139, 167, 248, 298, 2084, 2144, 2155
KasI GGCGCC 1 cut(s) 2154
KflI GGGWCCC 1 cut(s) 1020
Kpn2I TCCGGA 2 cut(s) 745, 1968
KspI CCGCGG 1 cut(s) 160
LguI GCTCTTC 2 cut(s) 671, 885
LmnI GCTCC 6 cut(s) 86, 159, 443, 748, 1276, 1807
Lsp1109I GCAGC 7 cut(s) 132, 237, 673, 1240, 1557, 1715, 1924
LweI GCATC 5 cut(s) 427, 1145, 1240, 1968, 2131
MaeII ACGT 4 cut(s) 555, 776, 918, 1300
MaeIII GTNAC 3 cut(s) 322, 1059, 1824
MbiI CCGCTC 3 cut(s) 81, 564, 851
MboII GAAGA 7 cut(s) 454, 658, 859, 902, 1219, 1834, 2150
MfeI CAATTG 1 cut(s) 739
MflI RGATCY 3 cut(s) 624, 802, 1665
MhlI GDGCHC 7 cut(s) 753, 903, 979, 1010, 1273, 1443, 1646
MlsI TGGCCA 4 cut(s) 788, 1324, 1524, 1891
MluCI AATT 6 cut(s) 113, 503, 739, 1164, 1281, 1715
MluNI TGGCCA 4 cut(s) 788, 1324, 1524, 1891
Mly113I GGCGCC 1 cut(s) 2155
MlyI GAGTC 8 cut(s) 384, 472, 765, 1067, 1399, 1876, 1930, 2114
MmeI TCCRAC 4 cut(s) 96, 234, 480, 1373
Mox20I TGGCCA 4 cut(s) 788, 1324, 1524, 1891
Mph1103I ATGCAT 1 cut(s) 1961
MroI TCCGGA 2 cut(s) 745, 1968
MroXI GAANNNNTTC 1 cut(s) 1976
MscI TGGCCA 4 cut(s) 788, 1324, 1524, 1891
MseI TTAA 3 cut(s) 981, 1071, 2048
MslI CAYNNNNRTG 1 cut(s) 1530
Msp20I TGGCCA 4 cut(s) 788, 1324, 1524, 1891
MspA1I CMGCKG 2 cut(s) 159, 1937
MspCI CTTAAG 2 cut(s) 980, 2047
MspI CCGG 9 cut(s) 35, 386, 533, 746, 873, 1669, 1903, 1941, 1969
MspR9I CCNGG 6 cut(s) 387, 874, 1247, 1670, 1809, 1904
MunI CAATTG 1 cut(s) 739
Mva1269I GAATGC 1 cut(s) 1961
MvaI CCWGG 2 cut(s) 1247, 1809
NarI GGCGCC 1 cut(s) 2155
NciI CCSGG 4 cut(s) 387, 874, 1670, 1904
NcoI CCATGG 3 cut(s) 997, 1525, 1984
NlaIII CATG 5 cut(s) 1001, 1414, 1529, 1988, 2011
NlaIV GGNNCC 8 cut(s) 173, 242, 445, 804, 1021, 1022, 1278, 2156
NmeAIII GCCGAG 2 cut(s) 989, 1726
NmuCI GTSAC 3 cut(s) 322, 1059, 1824
NruI TCGCGA 2 cut(s) 1115, 1287
NsiI ATGCAT 1 cut(s) 1961
PaeR7I CTCGAG 1 cut(s) 125
PagI TCATGA 1 cut(s) 2007
PceI AGGCCT 1 cut(s) 644
PciSI GCTCTTC 2 cut(s) 671, 885
PcsI WCGNNNNNNNCGW 1 cut(s) 1297
PctI GAATGC 1 cut(s) 1961
PdmI GAANNNNTTC 1 cut(s) 1976
PfeI GAWTC 3 cut(s) 43, 1294, 1660
PflFI GACNNNGTC 1 cut(s) 592
PflMI CCANNNNNTGG 2 cut(s) 381, 1502
Ple19I CGATCG 1 cut(s) 1291
PleI GAGTC 8 cut(s) 384, 472, 764, 1066, 1398, 1875, 1929, 2114
PluTI GGCGCC 1 cut(s) 2158
PpsI GAGTC 8 cut(s) 384, 472, 764, 1066, 1398, 1875, 1929, 2114
PpuMI RGGWCCY 2 cut(s) 172, 1020
PshAI GACNNNNGTC 1 cut(s) 1866
Psp124BI GAGCTC 2 cut(s) 753, 979
Psp5II RGGWCCY 2 cut(s) 172, 1020
Psp6I CCWGG 2 cut(s) 1245, 1807
PspFI CCCAGC 2 cut(s) 294, 1023
PspGI CCWGG 2 cut(s) 1245, 1807
PspN4I GGNNCC 8 cut(s) 173, 242, 445, 804, 1021, 1022, 1278, 2156
PspPI GGNCC 6 cut(s) 172, 241, 516, 559, 1020, 1414
PspPPI RGGWCCY 2 cut(s) 172, 1020
PstI CTGCAG 2 cut(s) 1084, 1258
PstNI CAGNNNCTG 1 cut(s) 2087
PsuI RGATCY 3 cut(s) 624, 802, 1665
PsyI GACNNNGTC 1 cut(s) 592
PvuI CGATCG 1 cut(s) 1291
PvuII CAGCTG 1 cut(s) 1937
RruI TCGCGA 2 cut(s) 1115, 1287
RsaI GTAC 2 cut(s) 1990, 2104
RsaNI GTAC 2 cut(s) 1989, 2103
RseI CAYNNNNRTG 1 cut(s) 1530
SacI GAGCTC 2 cut(s) 753, 979
SacII CCGCGG 1 cut(s) 160
SapI GCTCTTC 2 cut(s) 671, 885
SaqAI TTAA 3 cut(s) 981, 1071, 2048
Sau96I GGNCC 6 cut(s) 172, 241, 516, 559, 1020, 1414
SchI GAGTC 8 cut(s) 384, 472, 765, 1067, 1399, 1876, 1930, 2114
ScrFI CCNGG 6 cut(s) 387, 874, 1247, 1670, 1809, 1904
SduI GDGCHC 7 cut(s) 753, 903, 979, 1010, 1273, 1443, 1646
SfaNI GCATC 5 cut(s) 427, 1145, 1240, 1968, 2131
SfcI CTRYAG 2 cut(s) 1080, 1254
SfoI GGCGCC 1 cut(s) 2156
Sfr274I CTCGAG 1 cut(s) 125
Sfr303I CCGCGG 1 cut(s) 160
SgrBI CCGCGG 1 cut(s) 160
SinI GGWCC 4 cut(s) 172, 516, 1020, 1414
SlaI CTCGAG 1 cut(s) 125
SmiMI CAYNNNNRTG 1 cut(s) 1530
SmlI CTYRAG 5 cut(s) 125, 980, 1864, 1896, 2047
SmoI CTYRAG 5 cut(s) 125, 980, 1864, 1896, 2047
Sse9I AATT 6 cut(s) 113, 503, 739, 1164, 1281, 1715
SseBI AGGCCT 1 cut(s) 644
SspDI GGCGCC 1 cut(s) 2154
SstI GAGCTC 2 cut(s) 753, 979
StuI AGGCCT 1 cut(s) 644
StyD4I CCNGG 6 cut(s) 385, 872, 1245, 1668, 1807, 1902
StyI CCWWGG 4 cut(s) 939, 997, 1525, 1984
TaaI ACNGT 8 cut(s) 337, 361, 818, 929, 1065, 1171, 1484, 1783
TaiI ACGT 4 cut(s) 558, 779, 921, 1303
TaqII GACCGA 1 cut(s) 533
TasI AATT 6 cut(s) 113, 503, 739, 1164, 1281, 1715
TatI WGTACW 1 cut(s) 2102
TauI GCSGC 9 cut(s) 11, 14, 17, 53, 111, 162, 399, 430, 854
TfiI GAWTC 3 cut(s) 43, 1294, 1660
Tru1I TTAA 3 cut(s) 981, 1071, 2048
Tru9I TTAA 3 cut(s) 981, 1071, 2048
TscAI CASTG 5 cut(s) 934, 1068, 1176, 1269, 1800
TseFI GTSAC 3 cut(s) 322, 1059, 1824
TseI GCWGC 7 cut(s) 120, 250, 661, 1253, 1570, 1728, 1937
Tsp45I GTSAC 3 cut(s) 322, 1059, 1824
TspDTI ATGAA 5 cut(s) 1389, 1775, 1969, 1996, 2109
TspGWI ACGGA 2 cut(s) 193, 310
TspRI CASTG 5 cut(s) 934, 1068, 1176, 1269, 1800
Tth111I GACNNNGTC 1 cut(s) 592
Van91I CCANNNNNTGG 2 cut(s) 381, 1502
Vha464I CTTAAG 2 cut(s) 980, 2047
VpaK11BI GGWCC 4 cut(s) 172, 516, 1020, 1414
XagI CCTNNNNNAGG 1 cut(s) 35
XapI RAATTY 3 cut(s) 113, 503, 1715
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XhoI CTCGAG 1 cut(s) 125
XmiI GTMKAC 1 cut(s) 339
XmnI GAANNNNTTC 1 cut(s) 1976
Zsp2I ATGCAT 1 cut(s) 1961
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.