Rw2G031860

Bifunctional aspartate aminotransferase and glutamate aspartate-prephenate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0007427
Physical Location & Seq
Reverse (-)
52283309 .. 52289093
5785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G031860.1

Sequence Viewer

Length: 1185 bp
ATGGGTTCCCATCTAAAACTTGCAAAGAGGGCTGTGGACACTGATATGCCGGTCATGGTTCAGATACAACAATTGATCCGAGGAGCCAAAAATGCTGTGTCTCTGGCGCAGGGAGTAGTTCATTGGCAACCACCCAAACAGGCACTGGATAAGGTGAAAGAACTTGTATGGGAGCCTTCAATTAGTCGTTATGGTGCTGATGAAGGTATGCCTGAACTCAGGGAGGCATTGGTAAAAAAGTTGCAAGAGGAAAACAAGTTATGCAAATCTTCAGTTATGGTTACTGCTGGGGCAAATCAGGCATTTGTGAATCTTGTTCTTACATTGTGTGACGCTGGAGATTCTGTTGTCATGTTTGCACCATACTACTTCAATGCCTACATGTCCTTCCAGATGACTGGAGTTACTAATATACTTGTGGGTCCTGGTCAACCAGATACACTTTATCCAGATGCAGACTGGTTGGAGAAAACACTGTCAGAAACAAATCCAACCCCAAAACTTGTCACAGTTGTTAATCCTGGCAACCCATCCGGAACCTACATTCCAGAACCTCTTCTTAAGAGGATTTCAGATATTTGCAGAGAGGCTGGATCATGGCTTGTTGTGGACAATACATATGAGTATTTTATGTATGATGATTTAAAGCATTCTTGTGTGGAGGGTAATCACATTGTCAACATCTTTTCCTTCTCAAAAGCTTATGGGATGATGGGATGGCGAGTTGGCTATATAGCATACCCATCTGAAGTAGAGGGCTTTGGTACTCAACTCCTCAAAGTTCAAGATAACATTCCCATCTGTGCTTCAATACTTTCACAACATCTTGCCCTCTGCTCATTAGAAATGGGACCTGAATGGGTTGTTGAAAGAGTGAAAGGTCTTGTCAAGAACAGGGAAATTGTTTTAGAAGCGCTCTCCCCACTCGGAAAACAGTCTGTTAAAGGTGGAGAAGGTGCTATATACCTGTGGGCCAAGCTTCCAGACAAATATGTCAATGACTCCGAAATTGTTCACTGGCTTGCTCACAGGCATGGGGTGGTGGTGATTCCTGGAAGCGCATGTGGGTGCCCTGGAAATTTGAGAATCTCCTTTGGTGGCCTGTTAGAGGATGACTGCAAAGCTGCTGCAGAGAGGCTGAGAAGAGGGTTAGAAGAATTGGTCAAAGATGGAATGGTGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000096 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006520 GO:0006521 GO:0006555 GO:0006558 GO:0006568 GO:0006569 GO:0006570 GO:0006576 GO:0006586 GO:0006725 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008483 GO:0009056 GO:0009058 GO:0009063 GO:0009066 GO:0009072 GO:0009074 GO:0009308 GO:0009310 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009641 GO:0009698 GO:0009850 GO:0009851 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010252 GO:0010326 GO:0010364 GO:0010366 GO:0010565 GO:0010600 GO:0010817 GO:0016054 GO:0016740 GO:0016769 GO:0017144 GO:0019222 GO:0019439 GO:0019748 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031335 GO:0031336 GO:0032350 GO:0032351 GO:0032353 GO:0033238 GO:0033239 GO:0034641 GO:0042402 GO:0042430 GO:0042436 GO:0042445 GO:0042446 GO:0042592 GO:0042762 GO:0043436 GO:0044106 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0045763 GO:0046218 GO:0046395 GO:0046483 GO:0046700 GO:0046885 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051175 GO:0062012 GO:0062014 GO:0065007 GO:0065008 GO:0071704 GO:0080090 GO:0090354 GO:0090356 GO:0090357 GO:1900908 GO:1900909 GO:1900911 GO:1900912 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606 GO:1901996 GO:1901997 GO:1902221
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

394

Amino Acids

43.44

Weight (kDa)

5.7

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 33 - 380 1e-51 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 992
AccB1I GGYRCC 1 cut(s) 1068
AccIII TCCGGA 1 cut(s) 533
AclWI GGATC 2 cut(s) 70, 601
AcsI RAATTY 1 cut(s) 1078
AcuI CTGAAG 2 cut(s) 255, 768
AfaI GTAC 1 cut(s) 766
AfeI AGCGCT 1 cut(s) 915
AfiI CCNNNNNNNGG 1 cut(s) 1108
AflII CTTAAG 1 cut(s) 560
AflIII ACRYGT 1 cut(s) 381
AgsI TTSAA 5 cut(s) 180, 373, 785, 810, 869
AjnI CCWGG 4 cut(s) 424, 520, 1051, 1072
AloI GAACNNNNNNTCC 2 cut(s) 529, 561
AluBI AGCT 3 cut(s) 701, 979, 1124
AluI AGCT 3 cut(s) 701, 979, 1124
Alw26I GTCTC 1 cut(s) 105
AlwI GGATC 2 cut(s) 70, 601
AlwNI CAGNNNCTG 1 cut(s) 145
Aor13HI TCCGGA 1 cut(s) 533
Aor51HI AGCGCT 1 cut(s) 915
AoxI GGCC 2 cut(s) 972, 1099
ApeKI GCWGC 2 cut(s) 1124, 1127
ApoI RAATTY 1 cut(s) 1078
Asp700I GAANNNNTTC 2 cut(s) 555, 1011
AspLEI GCGC 3 cut(s) 109, 916, 1061
AspS9I GGNCC 3 cut(s) 422, 851, 972
AsuHPI GGTGA 2 cut(s) 166, 1057
AvaII GGWCC 2 cut(s) 422, 851
BaeGI GKGCMC 1 cut(s) 1073
BanI GGYRCC 1 cut(s) 1068
BbvI GCAGC 2 cut(s) 1111, 1114
BccI CCATC 7 cut(s) 18, 538, 706, 711, 751, 806, 1163
BciT130I CCWGG 4 cut(s) 426, 522, 1053, 1074
BcoDI GTCTC 1 cut(s) 105
BfmI CTRYAG 1 cut(s) 1128
BfoI RGCGCY 1 cut(s) 917
BfrI CTTAAG 1 cut(s) 560
BisI GCNGC 2 cut(s) 1125, 1128
BlsI GCNGC 2 cut(s) 1126, 1129
Bme1390I CCNGG 4 cut(s) 426, 522, 1053, 1074
Bme18I GGWCC 2 cut(s) 422, 851
BmgT120I GGNCC 3 cut(s) 422, 851, 972
BmiI GGNNCC 7 cut(s) 7, 85, 174, 423, 538, 852, 1070
BmrFI CCNGG 4 cut(s) 426, 522, 1053, 1074
BmsI GCATC 1 cut(s) 442
BpmI CTGGAG 2 cut(s) 357, 420
BsaJI CCNNGG 2 cut(s) 79, 1072
BsaWI WCCGGW 1 cut(s) 533
BsaXI ACNNNNNCTCC 2 cut(s) 330, 360
Bsc4I CCNNNNNNNGG 1 cut(s) 1108
Bse118I RCCGGY 1 cut(s) 49
Bse1I ACTGG 4 cut(s) 150, 403, 464, 1022
BseAI TCCGGA 1 cut(s) 533
BseBI CCWGG 4 cut(s) 426, 522, 1053, 1074
BseDI CCNNGG 2 cut(s) 79, 1072
BseGI GGATG 4 cut(s) 530, 714, 722, 1117
BseLI CCNNNNNNNGG 1 cut(s) 1108
BseMII CTCAG 2 cut(s) 232, 1130
BseNI ACTGG 4 cut(s) 150, 403, 464, 1022
BseRI GAGGAG 2 cut(s) 96, 764
BseSI GKGCMC 1 cut(s) 1073
BseXI GCAGC 2 cut(s) 1111, 1114
BseYI CCCAGC 1 cut(s) 287
BshFI GGCC 2 cut(s) 974, 1101
BshNI GGYRCC 1 cut(s) 1068
BsiSI CCGG 2 cut(s) 50, 534
BslFI GGGAC 1 cut(s) 864
BslI CCNNNNNNNGG 1 cut(s) 1108
BsmAI GTCTC 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 864
BsmI GAATGC 1 cut(s) 649
BsnI GGCC 2 cut(s) 974, 1101
Bsp1286I GDGCHC 1 cut(s) 1073
Bsp13I TCCGGA 1 cut(s) 533
Bsp143I GATC 2 cut(s) 75, 593
BspANI GGCC 2 cut(s) 974, 1101
BspCNI CTCAG 2 cut(s) 231, 1131
BspEI TCCGGA 1 cut(s) 533
BspLI GGNNCC 7 cut(s) 7, 85, 174, 423, 538, 852, 1070
BspMAI CTGCAG 1 cut(s) 1132
BspPI GGATC 2 cut(s) 70, 601
BspT107I GGYRCC 1 cut(s) 1068
BspTI CTTAAG 1 cut(s) 560
BsrFI RCCGGY 1 cut(s) 49
BsrI ACTGG 4 cut(s) 150, 403, 464, 1022
BssAI RCCGGY 1 cut(s) 49
BssECI CCNNGG 2 cut(s) 79, 1072
BssMI GATC 2 cut(s) 75, 593
Bst2UI CCWGG 4 cut(s) 426, 522, 1053, 1074
Bst4CI ACNGT 3 cut(s) 477, 511, 936
Bst6I CTCTTC 2 cut(s) 561, 1138
BstAFI CTTAAG 1 cut(s) 560
BstC8I GCNNGC 1 cut(s) 1023
BstDEI CTNAG 2 cut(s) 218, 1139
BstENI CCTNNNNNAGG 1 cut(s) 1106
BstF5I GGATG 4 cut(s) 530, 714, 722, 1117
BstH2I RGCGCY 1 cut(s) 917
BstHHI GCGC 3 cut(s) 109, 916, 1061
BstKTI GATC 2 cut(s) 78, 596
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 2 cut(s) 75, 593
BstMWI GCNNNNNNNGC 3 cut(s) 29, 92, 299
BstNI CCWGG 4 cut(s) 426, 522, 1053, 1074
BstNSI RCATGY 2 cut(s) 385, 1065
BstSCI CCNGG 4 cut(s) 424, 520, 1051, 1072
BstSFI CTRYAG 1 cut(s) 1128
BstSLI GKGCMC 1 cut(s) 1073
BstV1I GCAGC 2 cut(s) 1111, 1114
BstXI CCANNNNNNTGG 1 cut(s) 398
BsuRI GGCC 2 cut(s) 974, 1101
BtsCI GGATG 4 cut(s) 530, 714, 722, 1117
BtsIMutI CAGTG 4 cut(s) 39, 143, 473, 1015
Cac8I GCNNGC 1 cut(s) 1023
CaiI CAGNNNCTG 1 cut(s) 145
CfoI GCGC 3 cut(s) 109, 916, 1061
Cfr10I RCCGGY 1 cut(s) 49
Cfr13I GGNCC 3 cut(s) 422, 851, 972
CseI GACGC 1 cut(s) 341
Csp6I GTAC 1 cut(s) 765
CviAII CATG 6 cut(s) 55, 352, 382, 597, 1034, 1062
CviQI GTAC 1 cut(s) 765
DdeI CTNAG 2 cut(s) 218, 1139
DpnI GATC 2 cut(s) 77, 595
DpnII GATC 2 cut(s) 75, 593
DraI TTTAAA 1 cut(s) 645
DrdI GACNNNNNNGTC 1 cut(s) 992
DseDI GACNNNNNNGTC 1 cut(s) 992
Eam1104I CTCTTC 2 cut(s) 561, 1138
EarI CTCTTC 2 cut(s) 561, 1138
Eco47I GGWCC 2 cut(s) 422, 851
Eco47III AGCGCT 1 cut(s) 915
Eco57I CTGAAG 2 cut(s) 255, 768
EcoNI CCTNNNNNAGG 1 cut(s) 1106
EcoO109I RGGNCCY 2 cut(s) 422, 851
EcoRII CCWGG 4 cut(s) 424, 520, 1051, 1072
FaeI CATG 6 cut(s) 58, 355, 385, 600, 1037, 1065
FaqI GGGAC 1 cut(s) 864
FatI CATG 6 cut(s) 54, 351, 381, 596, 1033, 1061
FauNDI CATATG 1 cut(s) 619
Fnu4HI GCNGC 2 cut(s) 1125, 1128
FokI GGATG 4 cut(s) 517, 721, 729, 1124
Fsp4HI GCNGC 2 cut(s) 1125, 1128
GlaI GCGC 3 cut(s) 108, 915, 1060
GluI GCNGC 2 cut(s) 1125, 1128
GsaI CCCAGC 1 cut(s) 291
GsuI CTGGAG 2 cut(s) 357, 420
HaeII RGCGCY 1 cut(s) 917
HaeIII GGCC 2 cut(s) 974, 1101
HapII CCGG 2 cut(s) 50, 534
HgaI GACGC 1 cut(s) 341
HhaI GCGC 3 cut(s) 109, 916, 1061
Hin1II CATG 6 cut(s) 58, 355, 385, 600, 1037, 1065
Hin6I GCGC 3 cut(s) 107, 914, 1059
HinP1I GCGC 3 cut(s) 107, 914, 1059
HincII GTYRAC 2 cut(s) 431, 679
HindII GTYRAC 2 cut(s) 431, 679
HindIII AAGCTT 2 cut(s) 699, 977
HinfI GANTC 5 cut(s) 310, 341, 1001, 1048, 1086
HpaII CCGG 2 cut(s) 50, 534
HphI GGTGA 2 cut(s) 166, 1057
Hpy166II GTNNAC 5 cut(s) 37, 431, 610, 679, 1015
Hpy188I TCNGA 7 cut(s) 63, 80, 481, 574, 748, 929, 1006
Hpy188III TCNNGA 7 cut(s) 391, 449, 534, 548, 785, 889, 983
Hpy8I GTNNAC 5 cut(s) 37, 431, 610, 679, 1015
HpyAV CCTTC 5 cut(s) 186, 197, 397, 700, 947
HpyCH4III ACNGT 3 cut(s) 477, 511, 936
HpyCH4V TGCA 8 cut(s) 23, 244, 264, 359, 455, 582, 1119, 1130
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 92, 299
HpyF3I CTNAG 2 cut(s) 218, 1139
Hsp92II CATG 6 cut(s) 58, 355, 385, 600, 1037, 1065
HspAI GCGC 3 cut(s) 107, 914, 1059
Kpn2I TCCGGA 1 cut(s) 533
Kzo9I GATC 2 cut(s) 75, 593
LmnI GCTCC 2 cut(s) 83, 172
Lsp1109I GCAGC 2 cut(s) 1111, 1114
LweI GCATC 1 cut(s) 442
MaeIII GTNAC 4 cut(s) 280, 329, 403, 505
MalI GATC 2 cut(s) 77, 595
MboI GATC 2 cut(s) 75, 593
MboII GAAGA 4 cut(s) 261, 548, 1155, 1166
MfeI CAATTG 1 cut(s) 71
MhlI GDGCHC 1 cut(s) 1073
MluCI AATT 7 cut(s) 71, 180, 900, 1008, 1078, 1157, 1180
MlyI GAGTC 1 cut(s) 995
MmeI TCCRAC 2 cut(s) 444, 515
MroI TCCGGA 1 cut(s) 533
MroXI GAANNNNTTC 2 cut(s) 555, 1011
MseI TTAA 5 cut(s) 516, 561, 644, 942, 1183
MslI CAYNNNNRTG 4 cut(s) 44, 654, 1032, 1066
MspCI CTTAAG 1 cut(s) 560
MspI CCGG 2 cut(s) 50, 534
MspR9I CCNGG 4 cut(s) 426, 522, 1053, 1074
MunI CAATTG 1 cut(s) 71
Mva1269I GAATGC 1 cut(s) 649
MvaI CCWGG 4 cut(s) 426, 522, 1053, 1074
MwoI GCNNNNNNNGC 3 cut(s) 29, 92, 299
NdeI CATATG 1 cut(s) 619
NdeII GATC 2 cut(s) 75, 593
NlaIII CATG 6 cut(s) 58, 355, 385, 600, 1037, 1065
NlaIV GGNNCC 7 cut(s) 7, 85, 174, 423, 538, 852, 1070
NmuCI GTSAC 2 cut(s) 329, 505
NspI RCATGY 2 cut(s) 385, 1065
PciI ACATGT 1 cut(s) 381
PctI GAATGC 1 cut(s) 649
PdmI GAANNNNTTC 2 cut(s) 555, 1011
PfeI GAWTC 4 cut(s) 310, 341, 1048, 1086
PfoI TCCNGGA 1 cut(s) 1051
PkrI GCNGC 2 cut(s) 1126, 1129
PleI GAGTC 1 cut(s) 995
PpsI GAGTC 1 cut(s) 995
PpuMI RGGWCCY 2 cut(s) 422, 851
PscI ACATGT 1 cut(s) 381
Psp5II RGGWCCY 2 cut(s) 422, 851
Psp6I CCWGG 4 cut(s) 424, 520, 1051, 1072
PspFI CCCAGC 1 cut(s) 287
PspGI CCWGG 4 cut(s) 424, 520, 1051, 1072
PspN4I GGNNCC 7 cut(s) 7, 85, 174, 423, 538, 852, 1070
PspPI GGNCC 3 cut(s) 422, 851, 972
PspPPI RGGWCCY 2 cut(s) 422, 851
PstI CTGCAG 1 cut(s) 1132
PstNI CAGNNNCTG 1 cut(s) 145
RsaI GTAC 1 cut(s) 766
RsaNI GTAC 1 cut(s) 765
RseI CAYNNNNRTG 4 cut(s) 44, 654, 1032, 1066
SaqAI TTAA 5 cut(s) 516, 561, 644, 942, 1183
SatI GCNGC 2 cut(s) 1125, 1128
Sau3AI GATC 2 cut(s) 75, 593
Sau96I GGNCC 3 cut(s) 422, 851, 972
SchI GAGTC 1 cut(s) 995
ScrFI CCNGG 4 cut(s) 426, 522, 1053, 1074
SduI GDGCHC 1 cut(s) 1073
SfaNI GCATC 1 cut(s) 442
SfcI CTRYAG 1 cut(s) 1128
SinI GGWCC 2 cut(s) 422, 851
SmiMI CAYNNNNRTG 4 cut(s) 44, 654, 1032, 1066
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
Sse9I AATT 7 cut(s) 71, 180, 900, 1008, 1078, 1157, 1180
StyD4I CCNGG 4 cut(s) 424, 520, 1051, 1072
TaaI ACNGT 3 cut(s) 477, 511, 936
TasI AATT 7 cut(s) 71, 180, 900, 1008, 1078, 1157, 1180
TfiI GAWTC 4 cut(s) 310, 341, 1048, 1086
Tru1I TTAA 5 cut(s) 516, 561, 644, 942, 1183
Tru9I TTAA 5 cut(s) 516, 561, 644, 942, 1183
TscAI CASTG 4 cut(s) 46, 150, 480, 1022
TseFI GTSAC 2 cut(s) 329, 505
TseI GCWGC 2 cut(s) 1124, 1127
Tsp45I GTSAC 2 cut(s) 329, 505
TspDTI ATGAA 2 cut(s) 110, 216
TspRI CASTG 4 cut(s) 46, 150, 480, 1022
Vha464I CTTAAG 1 cut(s) 560
VpaK11BI GGWCC 2 cut(s) 422, 851
XagI CCTNNNNNAGG 1 cut(s) 1106
XapI RAATTY 1 cut(s) 1078
XceI RCATGY 2 cut(s) 385, 1065
XcmI CCANNNNNNNNNTGG 2 cut(s) 142, 456
XmnI GAANNNNTTC 2 cut(s) 555, 1011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.