MD17G1198700.v1.1

Severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007427
Physical Location & Seq
Forward (+)
23928546 .. 23934365
5820 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1198700.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGGGTTCCTATGGACAACTTGCAAGGAGGGCCGTGGAGACTGATATGCCGATCATGGTTCAGATACAGCAGTTGGTCCGAGGAGCCAAAAATGCTGTGTCATTGGCTCAGGGAGTAGTTCACTGGCAACCACCCAAAGAGGCATTGGATAAGGTGAAAGAACTTGTTTGGGAGCCTTCAATTAGTCGTTACGGTGCTGATGAAGGTTTACCTGAACTCAGGGAGGCATTAGTACAAAAGTTGCATCGTGAAAATAAGTTGTACAAATCTTCAGTGATGGTTACTGCAGGCGCGAATCAGGCATTTGTGAACCTTGCTCTTACATTGTGTGATGCCGGGGACTCTGTGGTTATGTTTGCACCATACTACTTCAATGCCTACATGTCCTTCCAAATGACGGGAGTCACTAATATACTCGTGGGTCCTGGTCACCCAAAGACACTTCACCCAGATGCAGACTGGTTGGAGAAAACATTATCAGAAACCAAACCAACCCCAAAACTTGTTACAGTTGTTAATCCTGGTAACCCAAGTGGAACCTACATTCCGGATTCTCTTCTTAAGAGGATTTCCAATTTATGCAGAGATGCTGGATCATGGCTAGTTGTGGATAATACGTATGAGTATTTTATGTATGATGATTTGAAACACACATGTGTGGAGGGAAATCACATAGTCAACATTTTTTCCTTCTCGAAAGCTTATGGAATGATGGGATGGCGTGTTGGATATATAGCTTACCCATCAGAAGTAGAGGGCTTTGGTACCCAACTCCTCAAAGTTCAGGACAACATTCCTATCTGTGCTTCAATAATTTCACAGCACCTTGCCCTCTACTCATTGGAAATGGGACCCGAATGGGTCATTGAACGAGTGAAAGGTCTAGTGAAGAACAGAGACATCGTTATAGAAGCGCTCTCTCCCCTTGGGGACAGTGCTGTTAAAGGCGGAGAGGGAGCTATATACTTGTGGGCAAAGCTTCCGGACAAATATGCAGATGATGATAAATTTGTTCACTGGCTTGCTCACAGGCATGGTGTGGTGGTGATCCCCGGAAGTGCTTGTGGGTGCCCGGGGAATGTGAGAATCTCCTTTGGTGGCTTGGTAGAGGATGACTGCAAAGCTGCTGCAGAGAGGCTGAGGAGAGGGTTAGAAGAGTTAATCAGAGATGGATTGGTTGAGTAA

Protein Analysis

395

Amino Acids

43.53

Weight (kDa)

5.85

Isoelectric Point (pI)

31.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 33 - 380 3.6e-53 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 764
AccB1I GGYRCC 2 cut(s) 764, 1068
AccII CGCG 1 cut(s) 293
AccIII TCCGGA 2 cut(s) 547, 982
AciI CCGC 1 cut(s) 948
AclWI GGATC 2 cut(s) 601, 1042
AcsI RAATTY 1 cut(s) 1007
AcuI CTGAAG 1 cut(s) 255
AfaI GTAC 3 cut(s) 234, 263, 766
AfeI AGCGCT 1 cut(s) 915
AfiI CCNNNNNNNGG 1 cut(s) 397
AflII CTTAAG 1 cut(s) 560
AflIII ACRYGT 2 cut(s) 381, 653
AgsI TTSAA 5 cut(s) 180, 373, 646, 810, 869
AjnI CCWGG 2 cut(s) 424, 520
AleI CACNNNNGTG 2 cut(s) 654, 656
AloI GAACNNNNNNTCC 2 cut(s) 529, 561
AluBI AGCT 5 cut(s) 701, 737, 959, 979, 1124
AluI AGCT 5 cut(s) 701, 737, 959, 979, 1124
Alw26I GTCTC 2 cut(s) 32, 891
AlwI GGATC 2 cut(s) 601, 1042
Ama87I CYCGRG 1 cut(s) 1072
Aor13HI TCCGGA 2 cut(s) 547, 982
Aor51HI AGCGCT 1 cut(s) 915
AoxI GGCC 1 cut(s) 30
ApeKI GCWGC 2 cut(s) 1124, 1127
ApoI RAATTY 1 cut(s) 1007
Asp718I GGTACC 1 cut(s) 764
AspLEI GCGC 2 cut(s) 293, 916
AspS9I GGNCC 4 cut(s) 30, 76, 422, 851
AsuC2I CCSGG 4 cut(s) 337, 1053, 1073, 1074
AsuHPI GGTGA 4 cut(s) 166, 422, 437, 1057
AvaI CYCGRG 1 cut(s) 1072
AvaII GGWCC 3 cut(s) 76, 422, 851
BaeGI GKGCMC 1 cut(s) 1073
BaeI ACNNNNGTAYC 2 cut(s) 56, 89
BanI GGYRCC 2 cut(s) 764, 1068
BauI CACGAG 1 cut(s) 416
BbvCI CCTCAGC 1 cut(s) 1139
BbvI GCAGC 2 cut(s) 1111, 1114
BccI CCATC 5 cut(s) 271, 706, 711, 751, 1163
BceAI ACGGC 1 cut(s) 17
BciT130I CCWGG 2 cut(s) 426, 522
BcnI CCSGG 4 cut(s) 337, 1053, 1073, 1074
BcoDI GTCTC 2 cut(s) 32, 891
BfaI CTAG 2 cut(s) 602, 884
BfmI CTRYAG 2 cut(s) 285, 1128
BfoI RGCGCY 1 cut(s) 917
BfrI CTTAAG 1 cut(s) 560
BisI GCNGC 2 cut(s) 1125, 1128
BlsI GCNGC 2 cut(s) 1126, 1129
Bme1390I CCNGG 6 cut(s) 337, 426, 522, 1053, 1073, 1074
Bme18I GGWCC 3 cut(s) 76, 422, 851
BmeT110I CYCGRG 1 cut(s) 1072
BmgT120I GGNCC 4 cut(s) 30, 76, 422, 851
BmiI GGNNCC 9 cut(s) 7, 85, 174, 423, 538, 766, 852, 853, 1070
BmrFI CCNGG 6 cut(s) 337, 426, 522, 1053, 1073, 1074
BmsI GCATC 4 cut(s) 253, 322, 442, 577
BoxI GACNNNNGTC 1 cut(s) 401
Bpu10I CCTNAGC 2 cut(s) 108, 1139
BpuMI CCSGG 4 cut(s) 337, 1053, 1073, 1074
BsaAI YACGTR 1 cut(s) 618
BsaJI CCNNGG 7 cut(s) 33, 79, 336, 925, 1051, 1072, 1073
BsaWI WCCGGW 2 cut(s) 547, 982
Bsc4I CCNNNNNNNGG 1 cut(s) 397
Bse1I ACTGG 3 cut(s) 128, 464, 1022
BseAI TCCGGA 2 cut(s) 547, 982
BseBI CCWGG 2 cut(s) 426, 522
BseDI CCNNGG 7 cut(s) 33, 79, 336, 925, 1051, 1072, 1073
BseGI GGATG 2 cut(s) 722, 1117
BseLI CCNNNNNNNGG 1 cut(s) 397
BseMII CTCAG 3 cut(s) 122, 232, 1130
BseNI ACTGG 3 cut(s) 128, 464, 1022
BseRI GAGGAG 3 cut(s) 96, 764, 1156
BseSI GKGCMC 1 cut(s) 1073
BseXI GCAGC 2 cut(s) 1111, 1114
Bsh1236I CGCG 1 cut(s) 293
BshFI GGCC 1 cut(s) 32
BshNI GGYRCC 2 cut(s) 764, 1068
BsiHKCI CYCGRG 1 cut(s) 1072
BsiSI CCGG 5 cut(s) 336, 548, 983, 1053, 1073
BslFI GGGAC 3 cut(s) 353, 864, 944
BslI CCNNNNNNNGG 1 cut(s) 397
BsmAI GTCTC 2 cut(s) 32, 891
BsmFI GGGAC 3 cut(s) 353, 864, 944
BsnI GGCC 1 cut(s) 32
BsoBI CYCGRG 1 cut(s) 1072
Bsp1286I GDGCHC 1 cut(s) 1073
Bsp13I TCCGGA 2 cut(s) 547, 982
Bsp1407I TGTACA 1 cut(s) 261
Bsp143I GATC 3 cut(s) 51, 593, 1047
BspACI CCGC 1 cut(s) 948
BspANI GGCC 1 cut(s) 32
BspCNI CTCAG 3 cut(s) 121, 231, 1131
BspEI TCCGGA 2 cut(s) 547, 982
BspFNI CGCG 1 cut(s) 293
BspLI GGNNCC 9 cut(s) 7, 85, 174, 423, 538, 766, 852, 853, 1070
BspMAI CTGCAG 2 cut(s) 289, 1132
BspPI GGATC 2 cut(s) 601, 1042
BspT107I GGYRCC 2 cut(s) 764, 1068
BspTI CTTAAG 1 cut(s) 560
BsrGI TGTACA 1 cut(s) 261
BsrI ACTGG 3 cut(s) 128, 464, 1022
BssECI CCNNGG 7 cut(s) 33, 79, 336, 925, 1051, 1072, 1073
BssMI GATC 3 cut(s) 51, 593, 1047
BssSI CACGAG 1 cut(s) 416
BssT1I CCWWGG 1 cut(s) 925
Bst2BI CACGAG 1 cut(s) 416
Bst2UI CCWGG 2 cut(s) 426, 522
Bst4CI ACNGT 3 cut(s) 194, 511, 935
Bst6I CTCTTC 2 cut(s) 561, 1149
BstAFI CTTAAG 1 cut(s) 560
BstAUI TGTACA 1 cut(s) 261
BstBAI YACGTR 1 cut(s) 618
BstC8I GCNNGC 2 cut(s) 289, 1023
BstDEI CTNAG 3 cut(s) 108, 218, 1139
BstDSI CCRYGG 1 cut(s) 33
BstEII GGTNACC 2 cut(s) 428, 524
BstF5I GGATG 2 cut(s) 722, 1117
BstFNI CGCG 1 cut(s) 293
BstH2I RGCGCY 1 cut(s) 917
BstHHI GCGC 2 cut(s) 293, 916
BstKTI GATC 3 cut(s) 54, 596, 1050
BstMAI GTCTC 2 cut(s) 32, 891
BstMBI GATC 3 cut(s) 51, 593, 1047
BstMWI GCNNNNNNNGC 3 cut(s) 29, 92, 299
BstNI CCWGG 2 cut(s) 426, 522
BstNSI RCATGY 2 cut(s) 385, 657
BstPAI GACNNNNGTC 1 cut(s) 401
BstPI GGTNACC 2 cut(s) 428, 524
BstSCI CCNGG 6 cut(s) 335, 424, 520, 1051, 1071, 1072
BstSFI CTRYAG 2 cut(s) 285, 1128
BstSLI GKGCMC 1 cut(s) 1073
BstSNI TACGTA 1 cut(s) 618
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 2 cut(s) 1111, 1114
BsuRI GGCC 1 cut(s) 32
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 2 cut(s) 722, 1117
BtsIMutI CAGTG 4 cut(s) 121, 279, 940, 1015
Cac8I GCNNGC 2 cut(s) 289, 1023
CfoI GCGC 2 cut(s) 293, 916
Cfr13I GGNCC 4 cut(s) 30, 76, 422, 851
Cfr9I CCCGGG 1 cut(s) 1072
Csp6I GTAC 3 cut(s) 233, 262, 765
CviAII CATG 5 cut(s) 55, 382, 597, 654, 1034
CviQI GTAC 3 cut(s) 233, 262, 765
DdeI CTNAG 3 cut(s) 108, 218, 1139
DpnI GATC 3 cut(s) 53, 595, 1049
DpnII GATC 3 cut(s) 51, 593, 1047
Eam1104I CTCTTC 2 cut(s) 561, 1149
EarI CTCTTC 2 cut(s) 561, 1149
EciI GGCGGA 1 cut(s) 963
Eco105I TACGTA 1 cut(s) 618
Eco130I CCWWGG 1 cut(s) 925
Eco47I GGWCC 3 cut(s) 76, 422, 851
Eco47III AGCGCT 1 cut(s) 915
Eco57I CTGAAG 1 cut(s) 255
Eco88I CYCGRG 1 cut(s) 1072
Eco91I GGTNACC 2 cut(s) 428, 524
EcoO109I RGGNCCY 2 cut(s) 422, 851
EcoO65I GGTNACC 2 cut(s) 428, 524
EcoRII CCWGG 2 cut(s) 424, 520
EcoT14I CCWWGG 1 cut(s) 925
ErhI CCWWGG 1 cut(s) 925
FaeI CATG 5 cut(s) 58, 385, 600, 657, 1037
FaqI GGGAC 3 cut(s) 353, 864, 944
FatI CATG 5 cut(s) 54, 381, 596, 653, 1033
Fnu4HI GCNGC 2 cut(s) 1125, 1128
FokI GGATG 2 cut(s) 729, 1124
Fsp4HI GCNGC 2 cut(s) 1125, 1128
FspBI CTAG 2 cut(s) 602, 884
GlaI GCGC 2 cut(s) 292, 915
GluI GCNGC 2 cut(s) 1125, 1128
HaeII RGCGCY 1 cut(s) 917
HaeIII GGCC 1 cut(s) 32
HapII CCGG 5 cut(s) 336, 548, 983, 1053, 1073
HhaI GCGC 2 cut(s) 293, 916
Hin1II CATG 5 cut(s) 58, 385, 600, 657, 1037
Hin6I GCGC 2 cut(s) 291, 914
HinP1I GCGC 2 cut(s) 291, 914
HincII GTYRAC 1 cut(s) 679
HindII GTYRAC 1 cut(s) 679
HindIII AAGCTT 2 cut(s) 699, 977
HinfI GANTC 5 cut(s) 295, 341, 402, 551, 1086
HpaII CCGG 5 cut(s) 336, 548, 983, 1053, 1073
HphI GGTGA 4 cut(s) 166, 422, 437, 1057
Hpy166II GTNNAC 5 cut(s) 121, 209, 310, 679, 1015
Hpy188I TCNGA 5 cut(s) 63, 80, 481, 748, 1166
Hpy188III TCNNGA 5 cut(s) 248, 548, 694, 785, 983
Hpy8I GTNNAC 5 cut(s) 121, 209, 310, 679, 1015
HpyAV CCTTC 4 cut(s) 186, 197, 397, 700
HpyCH4III ACNGT 3 cut(s) 194, 511, 935
HpyCH4IV ACGT 1 cut(s) 617
HpyCH4V TGCA 9 cut(s) 23, 244, 287, 359, 455, 582, 995, 1119, 1130
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 92, 299
HpyF3I CTNAG 3 cut(s) 108, 218, 1139
HpySE526I ACGT 1 cut(s) 617
Hsp92II CATG 5 cut(s) 58, 385, 600, 657, 1037
HspAI GCGC 2 cut(s) 291, 914
KflI GGGWCCC 1 cut(s) 851
Kpn2I TCCGGA 2 cut(s) 547, 982
KpnI GGTACC 1 cut(s) 768
Kzo9I GATC 3 cut(s) 51, 593, 1047
LmnI GCTCC 3 cut(s) 83, 172, 956
Lsp1109I GCAGC 2 cut(s) 1111, 1114
LweI GCATC 4 cut(s) 253, 322, 442, 577
MaeI CTAG 2 cut(s) 602, 884
MaeII ACGT 1 cut(s) 617
MaeIII GTNAC 6 cut(s) 188, 280, 403, 428, 505, 524
MalI GATC 3 cut(s) 53, 595, 1049
MboI GATC 3 cut(s) 51, 593, 1047
MboII GAAGA 4 cut(s) 261, 548, 901, 1166
MhlI GDGCHC 1 cut(s) 1073
MluCI AATT 4 cut(s) 180, 574, 813, 1007
MlyI GAGTC 2 cut(s) 335, 411
MmeI TCCRAC 2 cut(s) 444, 706
MroI TCCGGA 2 cut(s) 547, 982
MseI TTAA 4 cut(s) 516, 561, 942, 1160
MslI CAYNNNNRTG 4 cut(s) 450, 654, 656, 1032
MspCI CTTAAG 1 cut(s) 560
MspI CCGG 5 cut(s) 336, 548, 983, 1053, 1073
MspR9I CCNGG 6 cut(s) 337, 426, 522, 1053, 1073, 1074
MvaI CCWGG 2 cut(s) 426, 522
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 3 cut(s) 29, 92, 299
NciI CCSGG 4 cut(s) 337, 1053, 1073, 1074
NdeII GATC 3 cut(s) 51, 593, 1047
NlaIII CATG 5 cut(s) 58, 385, 600, 657, 1037
NlaIV GGNNCC 9 cut(s) 7, 85, 174, 423, 538, 766, 852, 853, 1070
NmuCI GTSAC 2 cut(s) 403, 428
NspI RCATGY 2 cut(s) 385, 657
OliI CACNNNNGTG 2 cut(s) 654, 656
PciI ACATGT 2 cut(s) 381, 653
PfeI GAWTC 3 cut(s) 295, 551, 1086
PkrI GCNGC 2 cut(s) 1126, 1129
PleI GAGTC 2 cut(s) 335, 410
PpsI GAGTC 2 cut(s) 335, 410
Ppu21I YACGTR 1 cut(s) 618
PpuMI RGGWCCY 2 cut(s) 422, 851
PscI ACATGT 2 cut(s) 381, 653
PshAI GACNNNNGTC 1 cut(s) 401
Psp5II RGGWCCY 2 cut(s) 422, 851
Psp6I CCWGG 2 cut(s) 424, 520
PspEI GGTNACC 2 cut(s) 428, 524
PspGI CCWGG 2 cut(s) 424, 520
PspN4I GGNNCC 9 cut(s) 7, 85, 174, 423, 538, 766, 852, 853, 1070
PspPI GGNCC 4 cut(s) 30, 76, 422, 851
PspPPI RGGWCCY 2 cut(s) 422, 851
PstI CTGCAG 2 cut(s) 289, 1132
RsaI GTAC 3 cut(s) 234, 263, 766
RsaNI GTAC 3 cut(s) 233, 262, 765
RseI CAYNNNNRTG 4 cut(s) 450, 654, 656, 1032
SaqAI TTAA 4 cut(s) 516, 561, 942, 1160
SatI GCNGC 2 cut(s) 1125, 1128
Sau3AI GATC 3 cut(s) 51, 593, 1047
Sau96I GGNCC 4 cut(s) 30, 76, 422, 851
SchI GAGTC 2 cut(s) 335, 411
ScrFI CCNGG 6 cut(s) 337, 426, 522, 1053, 1073, 1074
SduI GDGCHC 1 cut(s) 1073
SfaNI GCATC 4 cut(s) 253, 322, 442, 577
SfcI CTRYAG 2 cut(s) 285, 1128
SinI GGWCC 3 cut(s) 76, 422, 851
SmaI CCCGGG 1 cut(s) 1074
SmiMI CAYNNNNRTG 4 cut(s) 450, 654, 656, 1032
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
SnaBI TACGTA 1 cut(s) 618
Sse9I AATT 4 cut(s) 180, 574, 813, 1007
SsiI CCGC 1 cut(s) 948
SspMI CTAG 2 cut(s) 602, 884
StyD4I CCNGG 6 cut(s) 335, 424, 520, 1051, 1071, 1072
StyI CCWWGG 1 cut(s) 925
TaaI ACNGT 3 cut(s) 194, 511, 935
TaiI ACGT 1 cut(s) 620
TaqI TCGA 1 cut(s) 695
TasI AATT 4 cut(s) 180, 574, 813, 1007
TatI WGTACW 2 cut(s) 232, 261
TfiI GAWTC 3 cut(s) 295, 551, 1086
Tru1I TTAA 4 cut(s) 516, 561, 942, 1160
Tru9I TTAA 4 cut(s) 516, 561, 942, 1160
TscAI CASTG 4 cut(s) 128, 279, 940, 1022
TseFI GTSAC 2 cut(s) 403, 428
TseI GCWGC 2 cut(s) 1124, 1127
Tsp45I GTSAC 2 cut(s) 403, 428
TspDTI ATGAA 1 cut(s) 216
TspMI CCCGGG 1 cut(s) 1072
TspRI CASTG 4 cut(s) 128, 279, 940, 1022
Vha464I CTTAAG 1 cut(s) 560
VpaK11BI GGWCC 3 cut(s) 76, 422, 851
XapI RAATTY 1 cut(s) 1007
XceI RCATGY 2 cut(s) 385, 657
XcmI CCANNNNNNNNNTGG 2 cut(s) 142, 456
XmaI CCCGGG 1 cut(s) 1072
XspI CTAG 2 cut(s) 602, 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.