Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0010646

Physical Location & Seq

Genomic Coordinates Reverse (-)

29321677 .. 29323725

Gene Structure 2049 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1233300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1104 bp

ATGGGTGCTTCCTCTATATTTTCTGCAGCATTGATATTTTCTCTTTTGTGTCAAATAGGAGGAAGGGTGGTGAGTCAAGGGACAATAAAACATGTAAACAGTGCTGGTTCTGGGTCTGGGTCTAGGTGTGACCTTTTCCATGGGGAATGGGTATATGATAAGTCCTACCCTCTCTACAATTCAGTAGACTGTCCCTTCATTTGGAAGGAATTCGATTGCCAAAAGAATGGTCGACCAGACAATGAGTATCTCAACTACAGATGGCAGCCCACTTCATGCAAATTACCAACGTTGGATGGGAAAAGCTTTTTGGAAAAATTTAGAGGGAAGCGCATTCTATTTGTAGGGGACTCACTGAGCATAAATCAATGGCAGTCCCTCACATGTTTACTTCATAAATCAGTGCCACAAGCCAATTACACCTCAAACAGGGTTGGAGGTGTCTCCACATTCAAGTTCCCGGCCTACGACGTTTCGATCGTACTGTCCCGGAATGCCTTCCTAGTAGATATTGTGCATGAGAACAGCAGACGTGTTCTAATGCTCGACTCGATTCAGAATGGAAGTTATTGGAGAACCTTTGATGTCTTGGTCTTCAATACCTGGCATTGGTGGCTTCATACTGGAAGAAAACAGCCGTGGGAGGAAGTTCGATATGGCGTAAACAACGTACACGAAGACATTGATCGCATGATGGCATATGAGAAAGCTTTGAAGACATGGGCTAGGTGGGTGGACTCCAATGTGGATCCTGGTAAAACTAAGGTCTTCTTTCAAGGTGTTTCTCCAGATCATATGAGTCCAAGAGAATGGGGCGATGATGCAAAATCAGGGAACTGTTTTGGACAAAGGGCACCAGTGATGGGGGCACAATATCCAGGAGGTTCACATCCAGCACAAGTTGTTCTAGAAAACGTGCTGCGGACAATGTCAAAGCCAGTTCATTTGCTCAACATAACAACTCTTTCACAACTAAGGAAAGACGGCCACCCATCGGTCTATGGTTTCGGTGGCCACCGGAACATGGATTGCACCCATTGGTGTCTTCCCGGCGTTCCGGATAGTTGGAATCAAATTCTGTTTGCAGCACTCTTCCATAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

368

Amino Acids

41.66

Weight (kDa)

8.77

Isoelectric Point (pI)

36.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	42 - 94	4.1e-23	PMR5 N terminal Domain
PC-Esterase	PF13839	95 - 362	2.4e-90	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010646)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30900 AT2G30900
fragaria_vesca	FvH4_6g27910
malus_domestica	MD09G1233300.v1.1 MD17G1229600.v1.1 MD17G1229700.v1.1
prunus_persica	Prupe.3G107900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0131461 RchiOBHm_Chr2g0131471
rosa_laevigata	RLG00000019184
rosa_multiflora	Rmu_sc0002869.1_g000010
rosa_roxburghii	Rroxscaffold_2G00112850
rosa_rugosa	Rorug02G0296800 Rorug02G0296900 Rorug02G0297000
rosa_samantha	Rh2AG350500 Rh2BG357000 Rh2CG336100 Rh2DG374900
rosa_wichuraiana	Rw2G028440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	853
AccI	GTMKAC	2 cut(s)	186, 232
AccIII	TCCGGA	1 cut(s)	1057
AciI	CCGC	1 cut(s)	922
AclI	AACGTT	1 cut(s)	290
AclWI	GGATC	2 cut(s)	743, 756
AcoI	YGGCCR	2 cut(s)	985, 1012
AcsI	RAATTY	3 cut(s)	209, 317, 1074
AfaI	GTAC	2 cut(s)	483, 672
AfiI	CCNNNNNNNGG	6 cut(s)	201, 429, 609, 863, 994, 1024
AflIII	ACRYGT	3 cut(s)	91, 383, 532
AgsI	TTSAA	4 cut(s)	454, 598, 715, 776
AjiI	CACGTC	1 cut(s)	533
AjnI	CCWGG	3 cut(s)	602, 751, 877
AjuI	GAANNNNNNNTTGG	2 cut(s)	293, 325
AluBI	AGCT	3 cut(s)	306, 710, 1101
AluI	AGCT	3 cut(s)	306, 710, 1101
Alw26I	GTCTC	1 cut(s)	448
AlwI	GGATC	2 cut(s)	743, 756
Aor13HI	TCCGGA	1 cut(s)	1057
AoxI	GGCC	3 cut(s)	462, 985, 1012
ApeKI	GCWGC	4 cut(s)	26, 265, 919, 1085
ApoI	RAATTY	3 cut(s)	209, 317, 1074
ArsI	GACNNNNNNTTYG	2 cut(s)	214, 246
Asp700I	GAANNNNTTC	2 cut(s)	209, 497
AspLEI	GCGC	1 cut(s)	333
AsuC2I	CCSGG	3 cut(s)	461, 490, 1050
AsuHPI	GGTGA	1 cut(s)	82
BaeGI	GKGCMC	2 cut(s)	856, 871
BalI	TGGCCA	1 cut(s)	1014
BamHI	GGATCC	1 cut(s)	748
BanI	GGYRCC	1 cut(s)	853
BbsI	GAAGAC	5 cut(s)	586, 684, 722, 760, 1037
BbvI	GCAGC	4 cut(s)	38, 277, 906, 1097
BccI	CCATC	5 cut(s)	255, 290, 688, 856, 1000
BceAI	ACGGC	2 cut(s)	622, 1000
BciT130I	CCWGG	3 cut(s)	604, 753, 879
BcnI	CCSGG	3 cut(s)	461, 490, 1050
BcoDI	GTCTC	1 cut(s)	448
BfaI	CTAG	4 cut(s)	123, 503, 726, 908
BfmI	CTRYAG	2 cut(s)	24, 256
BisI	GCNGC	4 cut(s)	27, 266, 920, 1086
BlsI	GCNGC	4 cut(s)	28, 267, 921, 1087
Bme1390I	CCNGG	6 cut(s)	461, 490, 604, 753, 879, 1050
BmgBI	CACGTC	1 cut(s)	533
BmiI	GGNNCC	2 cut(s)	750, 855
BmrFI	CCNGG	6 cut(s)	461, 490, 604, 753, 879, 1050
BmsI	GCATC	1 cut(s)	811
BpiI	GAAGAC	5 cut(s)	586, 684, 722, 760, 1037
BpmI	CTGGAG	1 cut(s)	771
BpuMI	CCSGG	3 cut(s)	461, 490, 1050
BsaJI	CCNNGG	2 cut(s)	139, 638
BsaWI	WCCGGW	2 cut(s)	1017, 1057
BsaXI	ACNNNNNCTCC	2 cut(s)	51, 81
Bsc4I	CCNNNNNNNGG	6 cut(s)	201, 429, 609, 863, 994, 1024
Bse1I	ACTGG	3 cut(s)	628, 857, 938
BseAI	TCCGGA	1 cut(s)	1057
BseBI	CCWGG	3 cut(s)	604, 753, 879
BseDI	CCNNGG	2 cut(s)	139, 638
BseGI	GGATG	2 cut(s)	301, 889
BseLI	CCNNNNNNNGG	6 cut(s)	201, 429, 609, 863, 994, 1024
BseMII	CTCAG	1 cut(s)	347
BseNI	ACTGG	3 cut(s)	628, 857, 938
BseSI	GKGCMC	2 cut(s)	856, 871
BseXI	GCAGC	4 cut(s)	38, 277, 906, 1097
Bsh1285I	CGRYCG	1 cut(s)	480
BshFI	GGCC	3 cut(s)	464, 987, 1014
BshNI	GGYRCC	1 cut(s)	853
BsiEI	CGRYCG	1 cut(s)	480
BsiSI	CCGG	5 cut(s)	461, 490, 1018, 1050, 1058
BslFI	GGGAC	5 cut(s)	94, 177, 361, 362, 472
BslI	CCNNNNNNNGG	6 cut(s)	201, 429, 609, 863, 994, 1024
BsmAI	GTCTC	1 cut(s)	448
BsmFI	GGGAC	5 cut(s)	94, 177, 361, 362, 472
BsmI	GAATGC	2 cut(s)	333, 499
BsnI	GGCC	3 cut(s)	464, 987, 1014
Bsp1286I	GDGCHC	2 cut(s)	856, 871
Bsp13I	TCCGGA	1 cut(s)	1057
Bsp143I	GATC	4 cut(s)	477, 685, 748, 790
Bsp19I	CCATGG	1 cut(s)	139
BspACI	CCGC	1 cut(s)	922
BspANI	GGCC	3 cut(s)	464, 987, 1014
BspCNI	CTCAG	1 cut(s)	348
BspEI	TCCGGA	1 cut(s)	1057
BspLI	GGNNCC	2 cut(s)	750, 855
BspMAI	CTGCAG	1 cut(s)	28
BspPI	GGATC	2 cut(s)	743, 756
BspT107I	GGYRCC	1 cut(s)	853
BsrI	ACTGG	3 cut(s)	628, 857, 938
BssECI	CCNNGG	2 cut(s)	139, 638
BssMI	GATC	4 cut(s)	477, 685, 748, 790
BssT1I	CCWWGG	1 cut(s)	139
Bst2UI	CCWGG	3 cut(s)	604, 753, 879
Bst4CI	ACNGT	4 cut(s)	101, 191, 486, 839
Bst6I	CTCTTC	1 cut(s)	1097
BstDEI	CTNAG	3 cut(s)	356, 762, 974
BstDSI	CCRYGG	2 cut(s)	139, 638
BstENI	CCTNNNNNAGG	1 cut(s)	427
BstF5I	GGATG	2 cut(s)	301, 889
BstHHI	GCGC	1 cut(s)	333
BstKTI	GATC	4 cut(s)	480, 688, 751, 793
BstMAI	GTCTC	1 cut(s)	448
BstMBI	GATC	4 cut(s)	477, 685, 748, 790
BstMCI	CGRYCG	1 cut(s)	480
BstMWI	GCNNNNNNNGC	1 cut(s)	613
BstNI	CCWGG	3 cut(s)	604, 753, 879
BstNSI	RCATGY	2 cut(s)	95, 387
BstSCI	CCNGG	6 cut(s)	459, 488, 602, 751, 877, 1048
BstSFI	CTRYAG	2 cut(s)	24, 256
BstSLI	GKGCMC	2 cut(s)	856, 871
BstV1I	GCAGC	4 cut(s)	38, 277, 906, 1097
BstV2I	GAAGAC	5 cut(s)	586, 684, 722, 760, 1037
BstX2I	RGATCY	1 cut(s)	748
BstXI	CCANNNNNNTGG	2 cut(s)	227, 810
BstYI	RGATCY	1 cut(s)	748
BsuRI	GGCC	3 cut(s)	464, 987, 1014
BtgI	CCRYGG	2 cut(s)	139, 638
BtgZI	GCGATG	1 cut(s)	831
BtrI	CACGTC	1 cut(s)	533
BtsCI	GGATG	2 cut(s)	301, 889
BtsIMutI	CAGTG	4 cut(s)	106, 353, 408, 864
CfoI	GCGC	1 cut(s)	333
Csp6I	GTAC	2 cut(s)	482, 671
CviAII	CATG	8 cut(s)	92, 140, 276, 384, 518, 691, 720, 1024
CviQI	GTAC	2 cut(s)	482, 671
DdeI	CTNAG	3 cut(s)	356, 762, 974
DpnI	GATC	4 cut(s)	479, 687, 750, 792
DpnII	GATC	4 cut(s)	477, 685, 748, 790
EaeI	YGGCCR	2 cut(s)	985, 1012
Eam1104I	CTCTTC	1 cut(s)	1097
EarI	CTCTTC	1 cut(s)	1097
Eco130I	CCWWGG	1 cut(s)	139
EcoNI	CCTNNNNNAGG	1 cut(s)	427
EcoRI	GAATTC	1 cut(s)	209
EcoRII	CCWGG	3 cut(s)	602, 751, 877
EcoT14I	CCWWGG	1 cut(s)	139
ErhI	CCWWGG	1 cut(s)	139
FaeI	CATG	8 cut(s)	95, 143, 279, 387, 521, 694, 723, 1027
FalI	AAGNNNNNCTT	2 cut(s)	755, 787
FaqI	GGGAC	5 cut(s)	94, 177, 361, 362, 472
FatI	CATG	8 cut(s)	91, 139, 275, 383, 517, 690, 719, 1023
FauNDI	CATATG	2 cut(s)	700, 795
FblI	GTMKAC	2 cut(s)	186, 232
Fnu4HI	GCNGC	4 cut(s)	27, 266, 920, 1086
FokI	GGATG	2 cut(s)	308, 876
Fsp4HI	GCNGC	4 cut(s)	27, 266, 920, 1086
FspBI	CTAG	4 cut(s)	123, 503, 726, 908
GlaI	GCGC	1 cut(s)	332
GluI	GCNGC	4 cut(s)	27, 266, 920, 1086
GsuI	CTGGAG	1 cut(s)	771
HaeIII	GGCC	3 cut(s)	464, 987, 1014
HapII	CCGG	5 cut(s)	461, 490, 1018, 1050, 1058
HhaI	GCGC	1 cut(s)	333
Hin1II	CATG	8 cut(s)	95, 143, 279, 387, 521, 694, 723, 1027
Hin6I	GCGC	1 cut(s)	331
HinP1I	GCGC	1 cut(s)	331
HincII	GTYRAC	1 cut(s)	233
HindII	GTYRAC	1 cut(s)	233
HindIII	AAGCTT	2 cut(s)	304, 708
HinfI	GANTC	7 cut(s)	73, 350, 548, 553, 737, 799, 1069
HpaII	CCGG	5 cut(s)	461, 490, 1018, 1050, 1058
HphI	GGTGA	1 cut(s)	82
Hpy166II	GTNNAC	8 cut(s)	97, 187, 233, 389, 664, 673, 736, 887
Hpy188I	TCNGA	1 cut(s)	558
Hpy188III	TCNNGA	3 cut(s)	788, 908, 1058
Hpy8I	GTNNAC	8 cut(s)	97, 187, 233, 389, 664, 673, 736, 887
Hpy99I	CGWCG	1 cut(s)	473
HpyAV	CCTTC	4 cut(s)	57, 199, 205, 508
HpyCH4III	ACNGT	4 cut(s)	101, 191, 486, 839
HpyCH4IV	ACGT	5 cut(s)	290, 471, 532, 669, 915
HpyCH4V	TGCA	6 cut(s)	26, 279, 517, 824, 1032, 1085
HpyF10VI	GCNNNNNNNGC	1 cut(s)	613
HpyF3I	CTNAG	3 cut(s)	356, 762, 974
HpySE526I	ACGT	5 cut(s)	290, 471, 532, 669, 915
Hsp92II	CATG	8 cut(s)	95, 143, 279, 387, 521, 694, 723, 1027
HspAI	GCGC	1 cut(s)	331
Kpn2I	TCCGGA	1 cut(s)	1057
Kzo9I	GATC	4 cut(s)	477, 685, 748, 790
Lsp1109I	GCAGC	4 cut(s)	38, 277, 906, 1097
LweI	GCATC	1 cut(s)	811
MaeI	CTAG	4 cut(s)	123, 503, 726, 908
MaeII	ACGT	5 cut(s)	290, 471, 532, 669, 915
MaeIII	GTNAC	1 cut(s)	128
MalI	GATC	4 cut(s)	479, 687, 750, 792
MboI	GATC	4 cut(s)	477, 685, 748, 790
MboII	GAAGA	7 cut(s)	586, 639, 689, 727, 760, 1037, 1084
MflI	RGATCY	1 cut(s)	748
MhlI	GDGCHC	2 cut(s)	856, 871
MlsI	TGGCCA	1 cut(s)	1014
MluCI	AATT	6 cut(s)	178, 209, 281, 317, 415, 1074
MluNI	TGGCCA	1 cut(s)	1014
MlyI	GAGTC	5 cut(s)	82, 344, 542, 731, 808
MmeI	TCCRAC	3 cut(s)	273, 415, 1046
MnlI	CCTC	9 cut(s)	22, 53, 180, 317, 389, 431, 433, 637, 875
Mox20I	TGGCCA	1 cut(s)	1014
MroI	TCCGGA	1 cut(s)	1057
MroXI	GAANNNNTTC	2 cut(s)	209, 497
MscI	TGGCCA	1 cut(s)	1014
Msp20I	TGGCCA	1 cut(s)	1014
MspI	CCGG	5 cut(s)	461, 490, 1018, 1050, 1058
MspR9I	CCNGG	6 cut(s)	461, 490, 604, 753, 879, 1050
Mva1269I	GAATGC	2 cut(s)	333, 499
MvaI	CCWGG	3 cut(s)	604, 753, 879
MwoI	GCNNNNNNNGC	1 cut(s)	613
NciI	CCSGG	3 cut(s)	461, 490, 1050
NcoI	CCATGG	1 cut(s)	139
NdeI	CATATG	2 cut(s)	700, 795
NdeII	GATC	4 cut(s)	477, 685, 748, 790
NlaIII	CATG	8 cut(s)	95, 143, 279, 387, 521, 694, 723, 1027
NlaIV	GGNNCC	2 cut(s)	750, 855
NmuCI	GTSAC	1 cut(s)	128
NspI	RCATGY	2 cut(s)	95, 387
PciI	ACATGT	2 cut(s)	91, 383
PcsI	WCGNNNNNNNCGW	1 cut(s)	477
PctI	GAATGC	2 cut(s)	333, 499
PdmI	GAANNNNTTC	2 cut(s)	209, 497
PfeI	GAWTC	2 cut(s)	553, 1069
PflFI	GACNNNGTC	1 cut(s)	928
PfoI	TCCNGGA	2 cut(s)	488, 877
PkrI	GCNGC	4 cut(s)	28, 267, 921, 1087
Ple19I	CGATCG	1 cut(s)	480
PleI	GAGTC	5 cut(s)	81, 344, 542, 731, 807
PpsI	GAGTC	5 cut(s)	81, 344, 542, 731, 807
PscI	ACATGT	2 cut(s)	91, 383
Psp1406I	AACGTT	1 cut(s)	290
Psp6I	CCWGG	3 cut(s)	602, 751, 877
PspGI	CCWGG	3 cut(s)	602, 751, 877
PspN4I	GGNNCC	2 cut(s)	750, 855
PstI	CTGCAG	1 cut(s)	28
PsuI	RGATCY	1 cut(s)	748
PsyI	GACNNNGTC	1 cut(s)	928
PvuI	CGATCG	1 cut(s)	480
RsaI	GTAC	2 cut(s)	483, 672
RsaNI	GTAC	2 cut(s)	482, 671
SalI	GTCGAC	1 cut(s)	231
SatI	GCNGC	4 cut(s)	27, 266, 920, 1086
Sau3AI	GATC	4 cut(s)	477, 685, 748, 790
SchI	GAGTC	5 cut(s)	82, 344, 542, 731, 808
ScrFI	CCNGG	6 cut(s)	461, 490, 604, 753, 879, 1050
SduI	GDGCHC	2 cut(s)	856, 871
SfaNI	GCATC	1 cut(s)	811
SfcI	CTRYAG	2 cut(s)	24, 256
Sse9I	AATT	6 cut(s)	178, 209, 281, 317, 415, 1074
SsiI	CCGC	1 cut(s)	922
SspMI	CTAG	4 cut(s)	123, 503, 726, 908
StyD4I	CCNGG	6 cut(s)	459, 488, 602, 751, 877, 1048
StyI	CCWWGG	1 cut(s)	139
TaaI	ACNGT	4 cut(s)	101, 191, 486, 839
TaiI	ACGT	5 cut(s)	293, 474, 535, 672, 918
TaqI	TCGA	6 cut(s)	213, 232, 476, 546, 551, 652
TaqII	GACCGA	1 cut(s)	985
TasI	AATT	6 cut(s)	178, 209, 281, 317, 415, 1074
TfiI	GAWTC	2 cut(s)	553, 1069
TscAI	CASTG	4 cut(s)	106, 360, 408, 864
TseFI	GTSAC	1 cut(s)	128
TseI	GCWGC	4 cut(s)	26, 265, 919, 1085
Tsp45I	GTSAC	1 cut(s)	128
TspDTI	ATGAA	5 cut(s)	187, 264, 383, 608, 932
TspRI	CASTG	4 cut(s)	106, 360, 408, 864
Tth111I	GACNNNGTC	1 cut(s)	928
XagI	CCTNNNNNAGG	1 cut(s)	427
XapI	RAATTY	3 cut(s)	209, 317, 1074
XbaI	TCTAGA	1 cut(s)	907
XceI	RCATGY	2 cut(s)	95, 387
XmiI	GTMKAC	2 cut(s)	186, 232
XmnI	GAANNNNTTC	2 cut(s)	209, 497
XspI	CTAG	4 cut(s)	123, 503, 726, 908

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.