Rh2BG357000

Protein trichome birefringence-like 43

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0010646
Physical Location & Seq
Forward (+)
49238001 .. 49239328
1328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG357000.1

Sequence Viewer

Length: 549 bp
ATGGAAGTTACTGGAGAACCTTTGATGTTATCATCTTCAATACATGGCATTGGTGGCTTCATACTGGAAAAAAAACAACCGTGGGCTTTTGTTCGTTATGGACTGAATAATGCCCACAAAGACATGAATCGCATGGTGGCGTATGAGAAAGCTCTAAATACATGGAAGAGATGGGTGGAGTCAAGTGTGGACACTGATAAAACAAAGATCTTCTTCCAGGGTGTTTCTCCTGATCATGTGAATCCAAGAGAATGGGGTGATGATGCAAACTCAAAGAGTTGTTTGGGAGAAACTGAACCAGTGAAGGGGACACAATATCCAGGAGGTTCACACCCAGCAGAAGTTGTTTTAGAAAGGGTATTGAGAAAAATGTCAAAGCCAGTTCATTTGCTAAACATCACCAGACTTTCACAACTAAGAAAAGACAGTCACCCTTCAGCCTATGGCTTCAGCGGCCACCGTGGCATGGATTGTACCCACTGGTGTGTTCCCGGTGTTCCAGATACTTGGAACCAACTTCTATTTGCAGCACTCTTCCAGGGCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

182

Amino Acids

20.45

Weight (kDa)

8.43

Isoelectric Point (pI)

35.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 25 - 176 2e-46 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 453
AcoI YGGCCR 1 cut(s) 454
AcuI CTGAAG 2 cut(s) 420, 433
AfaI GTAC 1 cut(s) 475
AfiI CCNNNNNNNGG 2 cut(s) 305, 466
AgsI TTSAA 1 cut(s) 39
AjnI CCWGG 3 cut(s) 216, 319, 537
AleI CACNNNNGTG 1 cut(s) 483
AluBI AGCT 1 cut(s) 152
AluI AGCT 1 cut(s) 152
AoxI GGCC 1 cut(s) 454
ApeKI GCWGC 1 cut(s) 527
AsuC2I CCSGG 1 cut(s) 492
AsuHPI GGTGA 3 cut(s) 269, 391, 422
BanII GRGCYC 1 cut(s) 545
BbvI GCAGC 1 cut(s) 539
BccI CCATC 1 cut(s) 165
BciT130I CCWGG 3 cut(s) 218, 321, 539
BclI TGATCA 1 cut(s) 232
BcnI CCSGG 1 cut(s) 492
BglI GCCNNNNNGGC 1 cut(s) 462
BglII AGATCT 1 cut(s) 207
BisI GCNGC 2 cut(s) 454, 528
BlsI GCNGC 2 cut(s) 455, 529
Bme1390I CCNGG 4 cut(s) 218, 321, 492, 539
BmiI GGNNCC 1 cut(s) 512
BmrFI CCNGG 4 cut(s) 218, 321, 492, 539
BmsI GCATC 1 cut(s) 253
BpmI CTGGAG 1 cut(s) 33
BpuMI CCSGG 1 cut(s) 492
BsaJI CCNNGG 4 cut(s) 80, 217, 460, 538
Bsc4I CCNNNNNNNGG 2 cut(s) 305, 466
Bse1I ACTGG 5 cut(s) 16, 69, 299, 380, 485
BseBI CCWGG 3 cut(s) 218, 321, 539
BseDI CCNNGG 4 cut(s) 80, 217, 460, 538
BseLI CCNNNNNNNGG 2 cut(s) 305, 466
BseNI ACTGG 5 cut(s) 16, 69, 299, 380, 485
BseXI GCAGC 1 cut(s) 539
BseYI CCCAGC 1 cut(s) 334
BshFI GGCC 1 cut(s) 456
BsiSI CCGG 1 cut(s) 492
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 2 cut(s) 305, 466
BsmFI GGGAC 1 cut(s) 322
BsnI GGCC 1 cut(s) 456
Bsp1286I GDGCHC 1 cut(s) 545
Bsp143I GATC 2 cut(s) 207, 232
BspACI CCGC 1 cut(s) 453
BspANI GGCC 1 cut(s) 456
BspHI TCATGA 1 cut(s) 545
BspLI GGNNCC 1 cut(s) 512
BsrI ACTGG 5 cut(s) 16, 69, 299, 380, 485
BssECI CCNNGG 4 cut(s) 80, 217, 460, 538
BssMI GATC 2 cut(s) 207, 232
Bst2UI CCWGG 3 cut(s) 218, 321, 539
Bst4CI ACNGT 3 cut(s) 81, 428, 461
Bst6I CTCTTC 2 cut(s) 161, 539
BstDEI CTNAG 1 cut(s) 416
BstDSI CCRYGG 2 cut(s) 80, 460
BstKTI GATC 2 cut(s) 210, 235
BstMBI GATC 2 cut(s) 207, 232
BstMWI GCNNNNNNNGC 3 cut(s) 54, 453, 462
BstNI CCWGG 3 cut(s) 218, 321, 539
BstSCI CCNGG 4 cut(s) 216, 319, 490, 537
BstV1I GCAGC 1 cut(s) 539
BstX2I RGATCY 1 cut(s) 207
BstXI CCANNNNNNTGG 2 cut(s) 252, 507
BstYI RGATCY 1 cut(s) 207
BsuRI GGCC 1 cut(s) 456
BtgI CCRYGG 2 cut(s) 80, 460
BtsIMutI CAGTG 3 cut(s) 192, 306, 478
CciI TCATGA 1 cut(s) 545
Csp6I GTAC 1 cut(s) 474
CviAII CATG 7 cut(s) 44, 124, 133, 162, 236, 466, 546
CviJI RGCY 8 cut(s) 57, 86, 152, 379, 440, 447, 456, 543
CviKI_1 RGCY 8 cut(s) 57, 86, 152, 379, 440, 447, 456, 543
CviQI GTAC 1 cut(s) 474
DdeI CTNAG 1 cut(s) 416
DpnI GATC 2 cut(s) 209, 234
DpnII GATC 2 cut(s) 207, 232
EaeI YGGCCR 1 cut(s) 454
Eam1104I CTCTTC 2 cut(s) 161, 539
EarI CTCTTC 2 cut(s) 161, 539
Eco24I GRGCYC 1 cut(s) 545
Eco57I CTGAAG 2 cut(s) 420, 433
EcoRII CCWGG 3 cut(s) 216, 319, 537
EcoT38I GRGCYC 1 cut(s) 545
FaeI CATG 7 cut(s) 47, 127, 136, 165, 239, 469, 549
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 1 cut(s) 322
FatI CATG 7 cut(s) 43, 123, 132, 161, 235, 465, 545
FbaI TGATCA 1 cut(s) 232
Fnu4HI GCNGC 2 cut(s) 454, 528
FriOI GRGCYC 1 cut(s) 545
Fsp4HI GCNGC 2 cut(s) 454, 528
GluI GCNGC 2 cut(s) 454, 528
GsaI CCCAGC 1 cut(s) 338
GsuI CTGGAG 1 cut(s) 33
HaeIII GGCC 1 cut(s) 456
HapII CCGG 1 cut(s) 492
Hin1II CATG 7 cut(s) 47, 127, 136, 165, 239, 469, 549
HinfI GANTC 3 cut(s) 127, 179, 241
HpaII CCGG 1 cut(s) 492
HphI GGTGA 3 cut(s) 269, 391, 422
Hpy166II GTNNAC 2 cut(s) 190, 329
Hpy188III TCNNGA 3 cut(s) 230, 500, 546
Hpy8I GTNNAC 2 cut(s) 190, 329
HpyAV CCTTC 2 cut(s) 298, 444
HpyCH4III ACNGT 3 cut(s) 81, 428, 461
HpyCH4V TGCA 2 cut(s) 266, 527
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 453, 462
HpyF3I CTNAG 1 cut(s) 416
Hsp92II CATG 7 cut(s) 47, 127, 136, 165, 239, 469, 549
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 2 cut(s) 207, 232
Lsp1109I GCAGC 1 cut(s) 539
LweI GCATC 1 cut(s) 253
MaeIII GTNAC 2 cut(s) 7, 428
MalI GATC 2 cut(s) 209, 234
MboI GATC 2 cut(s) 207, 232
MboII GAAGA 5 cut(s) 27, 178, 202, 205, 526
MflI RGATCY 1 cut(s) 207
MhlI GDGCHC 1 cut(s) 545
MlyI GAGTC 1 cut(s) 188
MnlI CCTC 1 cut(s) 317
MslI CAYNNNNRTG 1 cut(s) 483
MspA1I CMGCKG 1 cut(s) 453
MspI CCGG 1 cut(s) 492
MspR9I CCNGG 4 cut(s) 218, 321, 492, 539
MvaI CCWGG 3 cut(s) 218, 321, 539
MwoI GCNNNNNNNGC 3 cut(s) 54, 453, 462
NciI CCSGG 1 cut(s) 492
NdeII GATC 2 cut(s) 207, 232
NlaIII CATG 7 cut(s) 47, 127, 136, 165, 239, 469, 549
NlaIV GGNNCC 1 cut(s) 512
NmuCI GTSAC 1 cut(s) 428
OliI CACNNNNGTG 1 cut(s) 483
PagI TCATGA 1 cut(s) 545
PfeI GAWTC 2 cut(s) 127, 241
PfoI TCCNGGA 1 cut(s) 319
PkrI GCNGC 2 cut(s) 455, 529
PleI GAGTC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 187
Psp6I CCWGG 3 cut(s) 216, 319, 537
PspFI CCCAGC 1 cut(s) 334
PspGI CCWGG 3 cut(s) 216, 319, 537
PspN4I GGNNCC 1 cut(s) 512
PsuI RGATCY 1 cut(s) 207
RsaI GTAC 1 cut(s) 475
RsaNI GTAC 1 cut(s) 474
RseI CAYNNNNRTG 1 cut(s) 483
SatI GCNGC 2 cut(s) 454, 528
Sau3AI GATC 2 cut(s) 207, 232
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 4 cut(s) 218, 321, 492, 539
SduI GDGCHC 1 cut(s) 545
SetI ASST 3 cut(s) 22, 154, 328
SfaNI GCATC 1 cut(s) 253
SmiMI CAYNNNNRTG 1 cut(s) 483
SsiI CCGC 1 cut(s) 453
StyD4I CCNGG 4 cut(s) 216, 319, 490, 537
TaaI ACNGT 3 cut(s) 81, 428, 461
TauI GCSGC 1 cut(s) 456
TfiI GAWTC 2 cut(s) 127, 241
TscAI CASTG 3 cut(s) 199, 306, 485
TseFI GTSAC 1 cut(s) 428
TseI GCWGC 1 cut(s) 527
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 3 cut(s) 49, 140, 374
TspRI CASTG 3 cut(s) 199, 306, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.