RchiOBHm_Chr2g0131461

Protein trichome birefringence-like 43

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0010646
Physical Location & Seq
Forward (+)
47690959 .. 47692769
1811 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50280

Sequence Viewer

Length: 624 bp
ATGGCTTGTTCTACTGCCATTGCTTTAGCAATATGCATTTTCTCTCTTTTTCACAATATAGGAGGGACGCTTAGTGATGGGGGCTGTGACCTTTCTCGAGGACAATGGGTCTATGATCCGTCCTATCCTCTCTACAGTTCCACAGACTGTCCCTTCATTTGGAAGGAGTTTGATTGCCAAAAGAATAGTCGACCAGACAATGAGTACCTCAAGTACAGATGGCAGCCCGCTTCTTGCCAGACACCAAGGTTCAGTGGTAGTAGATTTTTGGAAAGATTTAGAGGGAAGCGCATACTGTTTGTAGGAGACTCACTGAGTATGAATCAATGGCAGTCCCTCACATGCATGCTTTACAAATCAGTGCAAGCCAAATACACCATAAACAGGATTGGAGGCCTCTCCATATTCAGCTTTCCAGCATATGATGTTTCGATCATGCTGTCCCGAAATGCATTTCTAGTAGATACAGTGCATATGAGAAATGGGAATGTTCTGATGCTGGACTCAATTGAGAATGGAAGTTACTGGAGAACCTTTGATGTTATCATCTTCAATACATGGCATTGGTGGCTTCATACTGGAAAAAAAACAACCGTGGGCTTTTGTTCGTTATGGACTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

207

Amino Acids

23.91

Weight (kDa)

8.62

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 28 - 80 3.5e-22 PMR5 N terminal Domain
PC-Esterase PF13839 82 - 195 2.3e-36 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 190
AciI CCGC 1 cut(s) 228
AclWI GGATC 1 cut(s) 110
AfaI GTAC 2 cut(s) 206, 215
AfiI CCNNNNNNNGG 2 cut(s) 159, 384
AgsI TTSAA 1 cut(s) 553
AhdI GACNNNNNGTC 1 cut(s) 107
AluBI AGCT 1 cut(s) 411
AluI AGCT 1 cut(s) 411
Alw26I GTCTC 1 cut(s) 300
AlwI GGATC 1 cut(s) 110
Ama87I CYCGRG 1 cut(s) 96
AoxI GGCC 1 cut(s) 394
ApeKI GCWGC 1 cut(s) 223
ArsI GACNNNNNNTTYG 2 cut(s) 172, 204
AspLEI GCGC 1 cut(s) 291
AvaI CYCGRG 1 cut(s) 96
BbvI GCAGC 1 cut(s) 235
BccI CCATC 2 cut(s) 71, 213
BcoDI GTCTC 1 cut(s) 300
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 1 cut(s) 133
BisI GCNGC 1 cut(s) 224
BlsI GCNGC 1 cut(s) 225
BmeRI GACNNNNNGTC 1 cut(s) 107
BmeT110I CYCGRG 1 cut(s) 96
BmsI GCATC 1 cut(s) 486
BpmI CTGGAG 1 cut(s) 547
BpuEI CTTGAG 1 cut(s) 194
BsaJI CCNNGG 2 cut(s) 245, 594
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 384
Bse1I ACTGG 2 cut(s) 530, 583
Bse3DI GCAATG 1 cut(s) 18
BseDI CCNNGG 2 cut(s) 245, 594
BseLI CCNNNNNNNGG 2 cut(s) 159, 384
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 1 cut(s) 305
BseNI ACTGG 2 cut(s) 530, 583
BseXI GCAGC 1 cut(s) 235
BshFI GGCC 1 cut(s) 396
BsiHKCI CYCGRG 1 cut(s) 96
BslFI GGGAC 4 cut(s) 79, 135, 319, 427
BslI CCNNNNNNNGG 2 cut(s) 159, 384
BsmAI GTCTC 1 cut(s) 300
BsmFI GGGAC 4 cut(s) 79, 135, 319, 427
BsnI GGCC 1 cut(s) 396
BsoBI CYCGRG 1 cut(s) 96
Bsp143I GATC 2 cut(s) 115, 432
BspACI CCGC 1 cut(s) 228
BspANI GGCC 1 cut(s) 396
BspCNI CTCAG 1 cut(s) 306
BspPI GGATC 1 cut(s) 110
BsrDI GCAATG 1 cut(s) 18
BsrI ACTGG 2 cut(s) 530, 583
BssECI CCNNGG 2 cut(s) 245, 594
BssMI GATC 2 cut(s) 115, 432
BssT1I CCWWGG 1 cut(s) 245
Bst4CI ACNGT 5 cut(s) 137, 149, 297, 469, 595
BstC8I GCNNGC 3 cut(s) 228, 347, 366
BstDEI CTNAG 2 cut(s) 71, 314
BstDSI CCRYGG 1 cut(s) 594
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 2 cut(s) 118, 435
BstMAI GTCTC 1 cut(s) 300
BstMBI GATC 2 cut(s) 115, 432
BstMWI GCNNNNNNNGC 1 cut(s) 568
BstNSI RCATGY 2 cut(s) 345, 349
BstSFI CTRYAG 1 cut(s) 133
BstV1I GCAGC 1 cut(s) 235
BsuRI GGCC 1 cut(s) 396
BtgI CCRYGG 1 cut(s) 594
BtsIMutI CAGTG 4 cut(s) 259, 311, 366, 474
Cac8I GCNNGC 3 cut(s) 228, 347, 366
CfoI GCGC 1 cut(s) 291
CseI GACGC 1 cut(s) 76
Csp6I GTAC 2 cut(s) 205, 214
CviAII CATG 4 cut(s) 342, 346, 436, 558
CviJI RGCY 8 cut(s) 5, 84, 226, 368, 396, 411, 571, 600
CviKI_1 RGCY 8 cut(s) 5, 84, 226, 368, 396, 411, 571, 600
CviQI GTAC 2 cut(s) 205, 214
DdeI CTNAG 2 cut(s) 71, 314
DpnI GATC 2 cut(s) 117, 434
DpnII GATC 2 cut(s) 115, 432
DriI GACNNNNNGTC 1 cut(s) 107
Eam1105I GACNNNNNGTC 1 cut(s) 107
Eco130I CCWWGG 1 cut(s) 245
Eco147I AGGCCT 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 96
EcoT14I CCWWGG 1 cut(s) 245
EcoT22I ATGCAT 3 cut(s) 38, 347, 454
ErhI CCWWGG 1 cut(s) 245
FaeI CATG 4 cut(s) 345, 349, 439, 561
FaqI GGGAC 4 cut(s) 79, 135, 319, 427
FatI CATG 4 cut(s) 341, 345, 435, 557
FauI CCCGC 1 cut(s) 235
FauNDI CATATG 2 cut(s) 421, 474
FblI GTMKAC 1 cut(s) 190
Fnu4HI GCNGC 1 cut(s) 224
Fsp4HI GCNGC 1 cut(s) 224
FspBI CTAG 1 cut(s) 458
GlaI GCGC 1 cut(s) 290
GluI GCNGC 1 cut(s) 224
GsuI CTGGAG 1 cut(s) 547
HaeIII GGCC 1 cut(s) 396
HgaI GACGC 1 cut(s) 76
HhaI GCGC 1 cut(s) 291
Hin1II CATG 4 cut(s) 345, 349, 439, 561
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HincII GTYRAC 1 cut(s) 191
HindII GTYRAC 1 cut(s) 191
HinfI GANTC 3 cut(s) 308, 322, 503
Hpy166II GTNNAC 1 cut(s) 191
Hpy188I TCNGA 1 cut(s) 495
Hpy188III TCNNGA 2 cut(s) 96, 444
Hpy8I GTNNAC 1 cut(s) 191
HpyAV CCTTC 2 cut(s) 157, 163
HpyCH4III ACNGT 5 cut(s) 137, 149, 297, 469, 595
HpyCH4V TGCA 5 cut(s) 36, 345, 364, 452, 472
HpyF10VI GCNNNNNNNGC 1 cut(s) 568
HpyF3I CTNAG 2 cut(s) 71, 314
Hsp92II CATG 4 cut(s) 345, 349, 439, 561
HspAI GCGC 1 cut(s) 289
Kzo9I GATC 2 cut(s) 115, 432
LpnPI CCDG 7 cut(s) 207, 251, 370, 429, 485, 511, 564
Lsp1109I GCAGC 1 cut(s) 235
LweI GCATC 1 cut(s) 486
MaeI CTAG 1 cut(s) 458
MaeIII GTNAC 2 cut(s) 86, 521
MalI GATC 2 cut(s) 117, 434
MboI GATC 2 cut(s) 115, 432
MboII GAAGA 1 cut(s) 541
MfeI CAATTG 1 cut(s) 507
MluCI AATT 1 cut(s) 507
MlyI GAGTC 2 cut(s) 302, 497
MnlI CCTC 8 cut(s) 56, 92, 138, 218, 275, 347, 386, 407
Mph1103I ATGCAT 3 cut(s) 38, 347, 454
MslI CAYNNNNRTG 1 cut(s) 344
MunI CAATTG 1 cut(s) 507
MwoI GCNNNNNNNGC 1 cut(s) 568
NdeI CATATG 2 cut(s) 421, 474
NdeII GATC 2 cut(s) 115, 432
NlaIII CATG 4 cut(s) 345, 349, 439, 561
NmuCI GTSAC 1 cut(s) 86
NsiI ATGCAT 3 cut(s) 38, 347, 454
NspI RCATGY 2 cut(s) 345, 349
PaeI GCATGC 1 cut(s) 349
PaeR7I CTCGAG 1 cut(s) 96
PceI AGGCCT 1 cut(s) 396
PfeI GAWTC 1 cut(s) 322
PkrI GCNGC 1 cut(s) 225
PleI GAGTC 2 cut(s) 302, 497
PpsI GAGTC 2 cut(s) 302, 497
RsaI GTAC 2 cut(s) 206, 215
RsaNI GTAC 2 cut(s) 205, 214
RseI CAYNNNNRTG 1 cut(s) 344
SalI GTCGAC 1 cut(s) 189
SatI GCNGC 1 cut(s) 224
Sau3AI GATC 2 cut(s) 115, 432
SchI GAGTC 2 cut(s) 302, 497
SetI ASST 5 cut(s) 93, 210, 251, 413, 536
SfaNI GCATC 1 cut(s) 486
SfcI CTRYAG 1 cut(s) 133
Sfr274I CTCGAG 1 cut(s) 96
SlaI CTCGAG 1 cut(s) 96
SmiMI CAYNNNNRTG 1 cut(s) 344
SmlI CTYRAG 2 cut(s) 96, 209
SmoI CTYRAG 2 cut(s) 96, 209
SphI GCATGC 1 cut(s) 349
Sse9I AATT 1 cut(s) 507
SseBI AGGCCT 1 cut(s) 396
SsiI CCGC 1 cut(s) 228
SspMI CTAG 1 cut(s) 458
StuI AGGCCT 1 cut(s) 396
StyI CCWWGG 1 cut(s) 245
TaaI ACNGT 5 cut(s) 137, 149, 297, 469, 595
TaqI TCGA 3 cut(s) 97, 190, 431
TasI AATT 1 cut(s) 507
TatI WGTACW 1 cut(s) 213
TfiI GAWTC 1 cut(s) 322
TscAI CASTG 4 cut(s) 259, 318, 366, 474
TseFI GTSAC 1 cut(s) 86
TseI GCWGC 1 cut(s) 223
Tsp45I GTSAC 1 cut(s) 86
TspDTI ATGAA 3 cut(s) 145, 335, 563
TspGWI ACGGA 1 cut(s) 108
TspRI CASTG 4 cut(s) 259, 318, 366, 474
XceI RCATGY 2 cut(s) 345, 349
XhoI CTCGAG 1 cut(s) 96
XmiI GTMKAC 1 cut(s) 190
XspI CTAG 1 cut(s) 458
Zsp2I ATGCAT 3 cut(s) 38, 347, 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.