RchiOBHm_Chr2g0131471

Protein trichome birefringence-like 43

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0010646
Physical Location & Seq
Forward (+)
47692771 .. 47693359
589 bp
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UTR
Exon/CDS
Intron
PRQ50281

Sequence Viewer

Length: 426 bp
ATGAATCGCATGGTGGCGTATGAGAAAGCTCTAAATACATGGAAGAGATGGGTGGAGTCAAGTGTGGACACTGATAAAACAAAGATCTTCTTCCAGGGTGTTTCTCCTGATCATGTGAATCCAAGAGAATGGGGTGATGATGCAAACTCAAAGAGTTGTTTGGGAGAAACTGAACCAGTGAAGGGGACACAATATCCAGGAGGTTCACACCCAGCAGAAGTTGTTTTAGAAAGGGTATTGAGAAAAATGTCAAAGCCAGTTCATTTGCTAAACATCACCAGACTTTCACAACTAAGAAAAGACAGTCACCCTTCAGCCTATGGCTTCAGCGGCCACCGTGGCATGGATTGTACCCACTGGTGTGTTCCCGGTGTTCCAGATACTTGGAACCAACTTCTATTTGCAGCACTCTTCCAGGGCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

141

Amino Acids

15.9

Weight (kDa)

8.48

Isoelectric Point (pI)

37.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 1 - 135 4.7e-44 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 330
AcoI YGGCCR 1 cut(s) 331
AcuI CTGAAG 2 cut(s) 297, 310
AfaI GTAC 1 cut(s) 352
AfiI CCNNNNNNNGG 2 cut(s) 182, 343
AjnI CCWGG 3 cut(s) 93, 196, 414
AleI CACNNNNGTG 1 cut(s) 360
AluBI AGCT 1 cut(s) 29
AluI AGCT 1 cut(s) 29
AoxI GGCC 1 cut(s) 331
ApeKI GCWGC 1 cut(s) 404
AsuC2I CCSGG 1 cut(s) 369
AsuHPI GGTGA 3 cut(s) 146, 268, 299
BanII GRGCYC 1 cut(s) 422
BbvI GCAGC 1 cut(s) 416
BccI CCATC 1 cut(s) 42
BciT130I CCWGG 3 cut(s) 95, 198, 416
BclI TGATCA 1 cut(s) 109
BcnI CCSGG 1 cut(s) 369
BglI GCCNNNNNGGC 1 cut(s) 339
BglII AGATCT 1 cut(s) 84
BisI GCNGC 2 cut(s) 331, 405
BlsI GCNGC 2 cut(s) 332, 406
Bme1390I CCNGG 4 cut(s) 95, 198, 369, 416
BmiI GGNNCC 1 cut(s) 389
BmrFI CCNGG 4 cut(s) 95, 198, 369, 416
BmsI GCATC 1 cut(s) 130
BpuMI CCSGG 1 cut(s) 369
BsaJI CCNNGG 3 cut(s) 94, 337, 415
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 343
Bse1I ACTGG 3 cut(s) 176, 257, 362
BseBI CCWGG 3 cut(s) 95, 198, 416
BseDI CCNNGG 3 cut(s) 94, 337, 415
BseLI CCNNNNNNNGG 2 cut(s) 182, 343
BseNI ACTGG 3 cut(s) 176, 257, 362
BseXI GCAGC 1 cut(s) 416
BseYI CCCAGC 1 cut(s) 211
BshFI GGCC 1 cut(s) 333
BsiSI CCGG 1 cut(s) 369
BslFI GGGAC 1 cut(s) 199
BslI CCNNNNNNNGG 2 cut(s) 182, 343
BsmFI GGGAC 1 cut(s) 199
BsnI GGCC 1 cut(s) 333
Bsp1286I GDGCHC 1 cut(s) 422
Bsp143I GATC 2 cut(s) 84, 109
BspACI CCGC 1 cut(s) 330
BspANI GGCC 1 cut(s) 333
BspHI TCATGA 1 cut(s) 422
BspLI GGNNCC 1 cut(s) 389
BsrI ACTGG 3 cut(s) 176, 257, 362
BssECI CCNNGG 3 cut(s) 94, 337, 415
BssMI GATC 2 cut(s) 84, 109
Bst2UI CCWGG 3 cut(s) 95, 198, 416
Bst4CI ACNGT 2 cut(s) 305, 338
Bst6I CTCTTC 2 cut(s) 38, 416
BstDEI CTNAG 1 cut(s) 293
BstDSI CCRYGG 1 cut(s) 337
BstKTI GATC 2 cut(s) 87, 112
BstMBI GATC 2 cut(s) 84, 109
BstMWI GCNNNNNNNGC 2 cut(s) 330, 339
BstNI CCWGG 3 cut(s) 95, 198, 416
BstSCI CCNGG 4 cut(s) 93, 196, 367, 414
BstV1I GCAGC 1 cut(s) 416
BstX2I RGATCY 1 cut(s) 84
BstXI CCANNNNNNTGG 2 cut(s) 129, 384
BstYI RGATCY 1 cut(s) 84
BsuRI GGCC 1 cut(s) 333
BtgI CCRYGG 1 cut(s) 337
BtsIMutI CAGTG 3 cut(s) 69, 183, 355
CciI TCATGA 1 cut(s) 422
Csp6I GTAC 1 cut(s) 351
CviAII CATG 5 cut(s) 10, 39, 113, 343, 423
CviJI RGCY 6 cut(s) 29, 256, 317, 324, 333, 420
CviKI_1 RGCY 6 cut(s) 29, 256, 317, 324, 333, 420
CviQI GTAC 1 cut(s) 351
DdeI CTNAG 1 cut(s) 293
DpnI GATC 2 cut(s) 86, 111
DpnII GATC 2 cut(s) 84, 109
EaeI YGGCCR 1 cut(s) 331
Eam1104I CTCTTC 2 cut(s) 38, 416
EarI CTCTTC 2 cut(s) 38, 416
Eco24I GRGCYC 1 cut(s) 422
Eco57I CTGAAG 2 cut(s) 297, 310
EcoRII CCWGG 3 cut(s) 93, 196, 414
EcoT38I GRGCYC 1 cut(s) 422
FaeI CATG 5 cut(s) 13, 42, 116, 346, 426
FaiI YATR 7 cut(s) 11, 21, 40, 114, 321, 344, 424
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FaqI GGGAC 1 cut(s) 199
FatI CATG 5 cut(s) 9, 38, 112, 342, 422
FbaI TGATCA 1 cut(s) 109
Fnu4HI GCNGC 2 cut(s) 331, 405
FriOI GRGCYC 1 cut(s) 422
Fsp4HI GCNGC 2 cut(s) 331, 405
GluI GCNGC 2 cut(s) 331, 405
GsaI CCCAGC 1 cut(s) 215
HaeIII GGCC 1 cut(s) 333
HapII CCGG 1 cut(s) 369
Hin1II CATG 5 cut(s) 13, 42, 116, 346, 426
HinfI GANTC 3 cut(s) 4, 56, 118
HpaII CCGG 1 cut(s) 369
HphI GGTGA 3 cut(s) 146, 268, 299
Hpy166II GTNNAC 2 cut(s) 67, 206
Hpy188III TCNNGA 3 cut(s) 107, 377, 423
Hpy8I GTNNAC 2 cut(s) 67, 206
HpyAV CCTTC 2 cut(s) 175, 321
HpyCH4III ACNGT 2 cut(s) 305, 338
HpyCH4V TGCA 2 cut(s) 143, 404
HpyF10VI GCNNNNNNNGC 2 cut(s) 330, 339
HpyF3I CTNAG 1 cut(s) 293
Hsp92II CATG 5 cut(s) 13, 42, 116, 346, 426
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 2 cut(s) 84, 109
Lsp1109I GCAGC 1 cut(s) 416
LweI GCATC 1 cut(s) 130
MaeIII GTNAC 1 cut(s) 305
MalI GATC 2 cut(s) 86, 111
MboI GATC 2 cut(s) 84, 109
MboII GAAGA 4 cut(s) 55, 79, 82, 403
MflI RGATCY 1 cut(s) 84
MhlI GDGCHC 1 cut(s) 422
MlyI GAGTC 1 cut(s) 65
MnlI CCTC 1 cut(s) 194
MslI CAYNNNNRTG 1 cut(s) 360
MspA1I CMGCKG 1 cut(s) 330
MspI CCGG 1 cut(s) 369
MspR9I CCNGG 4 cut(s) 95, 198, 369, 416
MvaI CCWGG 3 cut(s) 95, 198, 416
MwoI GCNNNNNNNGC 2 cut(s) 330, 339
NciI CCSGG 1 cut(s) 369
NdeII GATC 2 cut(s) 84, 109
NlaIII CATG 5 cut(s) 13, 42, 116, 346, 426
NlaIV GGNNCC 1 cut(s) 389
NmuCI GTSAC 1 cut(s) 305
OliI CACNNNNGTG 1 cut(s) 360
PagI TCATGA 1 cut(s) 422
PfeI GAWTC 2 cut(s) 4, 118
PfoI TCCNGGA 1 cut(s) 196
PkrI GCNGC 2 cut(s) 332, 406
PleI GAGTC 1 cut(s) 64
PpsI GAGTC 1 cut(s) 64
Psp6I CCWGG 3 cut(s) 93, 196, 414
PspFI CCCAGC 1 cut(s) 211
PspGI CCWGG 3 cut(s) 93, 196, 414
PspN4I GGNNCC 1 cut(s) 389
PsuI RGATCY 1 cut(s) 84
RsaI GTAC 1 cut(s) 352
RsaNI GTAC 1 cut(s) 351
RseI CAYNNNNRTG 1 cut(s) 360
SatI GCNGC 2 cut(s) 331, 405
Sau3AI GATC 2 cut(s) 84, 109
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 4 cut(s) 95, 198, 369, 416
SduI GDGCHC 1 cut(s) 422
SetI ASST 2 cut(s) 31, 205
SfaNI GCATC 1 cut(s) 130
SmiMI CAYNNNNRTG 1 cut(s) 360
SsiI CCGC 1 cut(s) 330
StyD4I CCNGG 4 cut(s) 93, 196, 367, 414
TaaI ACNGT 2 cut(s) 305, 338
TauI GCSGC 1 cut(s) 333
TfiI GAWTC 2 cut(s) 4, 118
TscAI CASTG 3 cut(s) 76, 183, 362
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 2 cut(s) 17, 251
TspRI CASTG 3 cut(s) 76, 183, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.