MD17G1229600.v1.1

Protein trichome birefringence-like 43

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010646
Physical Location & Seq
Reverse (-)
27772237 .. 27773218
982 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1229600.v1.1.491

Sequence Viewer

Length: 483 bp
ATGGGAGAGGCAGTTTTACTCTTGGTAAAAGAGAGTAATGGAAGCCCAGACCCAAAGAAAAATGTTGACAAGGAGTATCAGAAATACAGATGGCAGCCAACTTCGTGCCAGATACCGAGTTTTCTTCAAAGATTGAGAGGGAAGTGCATCCTGTTTGTAGGAGACTTATTGAGCTTGAATCAATGGCAGTTTCTCACATGCATGATTCACAAATCAGCACCAGAAGCTAAGTACGACCTAGTCAAGATTGGAGGCCTATCTGTACTCAAATTTCCAGCATATGACATCTCGATCATGCTCTCGCGCAATGCCTTTCTAGTAGATATCGTCCTTGAGGGAAGCCGGCGTGTTCTAGTGCTTAATTCGATTCAAAGTGGAAATTTTTGGAGAACCTTTGATGTCTTGGTCTTTGATACATGGCACTGGTGGCTTCATACCGGAAGGAAACAACCGTACGTTATAGATCATATGATAGTATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

161

Amino Acids

18.55

Weight (kDa)

9.1

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 40 - 148 2.2e-26 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 304
AcsI RAATTY 2 cut(s) 269, 379
AfaI GTAC 3 cut(s) 233, 264, 455
AgsI TTSAA 3 cut(s) 128, 178, 371
AluBI AGCT 2 cut(s) 174, 227
AluI AGCT 2 cut(s) 174, 227
Alw26I GTCTC 1 cut(s) 156
AoxI GGCC 1 cut(s) 253
ApeKI GCWGC 1 cut(s) 94
ApoI RAATTY 2 cut(s) 269, 379
AspLEI GCGC 1 cut(s) 306
BbvI GCAGC 1 cut(s) 106
BccI CCATC 1 cut(s) 84
BcoDI GTCTC 1 cut(s) 156
BfaI CTAG 3 cut(s) 239, 317, 353
BisI GCNGC 1 cut(s) 95
BlsI GCNGC 1 cut(s) 96
BmsI GCATC 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 353
BsaWI WCCGGW 1 cut(s) 437
BsaXI ACNNNNNCTCC 1 cut(s) 27
Bse118I RCCGGY 1 cut(s) 342
Bse1I ACTGG 1 cut(s) 428
Bse3DI GCAATG 1 cut(s) 313
BseGI GGATG 1 cut(s) 147
BseMI GCAATG 1 cut(s) 313
BseNI ACTGG 1 cut(s) 428
BseXI GCAGC 1 cut(s) 106
Bsh1236I CGCG 1 cut(s) 304
BshFI GGCC 1 cut(s) 255
BsiSI CCGG 2 cut(s) 343, 438
BsiWI CGTACG 1 cut(s) 453
BsmAI GTCTC 1 cut(s) 156
BsnI GGCC 1 cut(s) 255
Bsp143I GATC 2 cut(s) 291, 463
BspANI GGCC 1 cut(s) 255
BspFNI CGCG 1 cut(s) 304
BsrDI GCAATG 1 cut(s) 313
BsrFI RCCGGY 1 cut(s) 342
BsrI ACTGG 1 cut(s) 428
BssAI RCCGGY 1 cut(s) 342
BssMI GATC 2 cut(s) 291, 463
Bst4CI ACNGT 1 cut(s) 453
BstC8I GCNNGC 1 cut(s) 344
BstDEI CTNAG 1 cut(s) 228
BstF5I GGATG 1 cut(s) 147
BstFNI CGCG 1 cut(s) 304
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 2 cut(s) 294, 466
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 2 cut(s) 291, 463
BstMWI GCNNNNNNNGC 2 cut(s) 224, 427
BstNSI RCATGY 1 cut(s) 201
BstUI CGCG 1 cut(s) 304
BstV1I GCAGC 1 cut(s) 106
BsuRI GGCC 1 cut(s) 255
BtsCI GGATG 1 cut(s) 147
BtsIMutI CAGTG 1 cut(s) 421
Cac8I GCNNGC 1 cut(s) 344
CfoI GCGC 1 cut(s) 306
Cfr10I RCCGGY 1 cut(s) 342
Csp6I GTAC 3 cut(s) 232, 263, 454
CviAII CATG 4 cut(s) 198, 202, 295, 417
CviJI RGCY 7 cut(s) 45, 97, 174, 227, 255, 342, 430
CviKI_1 RGCY 7 cut(s) 45, 97, 174, 227, 255, 342, 430
CviQI GTAC 3 cut(s) 232, 263, 454
DdeI CTNAG 1 cut(s) 228
DpnI GATC 2 cut(s) 293, 465
DpnII GATC 2 cut(s) 291, 463
Eco147I AGGCCT 1 cut(s) 255
Eco32I GATATC 1 cut(s) 325
EcoRV GATATC 1 cut(s) 325
EcoT22I ATGCAT 1 cut(s) 203
FaeI CATG 4 cut(s) 201, 205, 298, 420
FatI CATG 4 cut(s) 197, 201, 294, 416
FauNDI CATATG 2 cut(s) 280, 468
Fnu4HI GCNGC 1 cut(s) 95
FokI GGATG 1 cut(s) 134
Fsp4HI GCNGC 1 cut(s) 95
FspBI CTAG 3 cut(s) 239, 317, 353
GlaI GCGC 1 cut(s) 305
GluI GCNGC 1 cut(s) 95
HaeIII GGCC 1 cut(s) 255
HapII CCGG 2 cut(s) 343, 438
HhaI GCGC 1 cut(s) 306
Hin1II CATG 4 cut(s) 201, 205, 298, 420
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HinfI GANTC 3 cut(s) 178, 205, 367
HpaII CCGG 2 cut(s) 343, 438
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 1 cut(s) 81
Hpy188III TCNNGA 2 cut(s) 244, 289
Hpy8I GTNNAC 1 cut(s) 67
HpyAV CCTTC 1 cut(s) 435
HpyCH4III ACNGT 1 cut(s) 453
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 2 cut(s) 147, 201
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 427
HpyF3I CTNAG 1 cut(s) 228
HpySE526I ACGT 1 cut(s) 456
Hsp92II CATG 4 cut(s) 201, 205, 298, 420
HspAI GCGC 1 cut(s) 304
KroI GCCGGC 1 cut(s) 342
KroNI GCCGGC 1 cut(s) 344
Kzo9I GATC 2 cut(s) 291, 463
LpnPI CCDG 8 cut(s) 60, 122, 164, 234, 288, 356, 409, 451
Lsp1109I GCAGC 1 cut(s) 106
LweI GCATC 1 cut(s) 156
MaeI CTAG 3 cut(s) 239, 317, 353
MaeII ACGT 1 cut(s) 456
MalI GATC 2 cut(s) 293, 465
MboI GATC 2 cut(s) 291, 463
MboII GAAGA 1 cut(s) 116
MluCI AATT 3 cut(s) 269, 361, 379
MnlI CCTC 3 cut(s) 131, 245, 328
Mph1103I ATGCAT 1 cut(s) 203
MroNI GCCGGC 1 cut(s) 342
MseI TTAA 2 cut(s) 360, 481
MslI CAYNNNNRTG 1 cut(s) 200
MspI CCGG 2 cut(s) 343, 438
MvnI CGCG 1 cut(s) 304
MwoI GCNNNNNNNGC 2 cut(s) 224, 427
NaeI GCCGGC 1 cut(s) 344
NdeI CATATG 2 cut(s) 280, 468
NdeII GATC 2 cut(s) 291, 463
NgoMIV GCCGGC 1 cut(s) 342
NlaIII CATG 4 cut(s) 201, 205, 298, 420
NsiI ATGCAT 1 cut(s) 203
NspI RCATGY 1 cut(s) 201
PceI AGGCCT 1 cut(s) 255
PdiI GCCGGC 1 cut(s) 344
PfeI GAWTC 3 cut(s) 178, 205, 367
Pfl23II CGTACG 1 cut(s) 453
PflFI GACNNNGTC 1 cut(s) 239
PkrI GCNGC 1 cut(s) 96
PspLI CGTACG 1 cut(s) 453
PsyI GACNNNGTC 1 cut(s) 239
RsaI GTAC 3 cut(s) 233, 264, 455
RsaNI GTAC 3 cut(s) 232, 263, 454
RseI CAYNNNNRTG 1 cut(s) 200
SaqAI TTAA 2 cut(s) 360, 481
SatI GCNGC 1 cut(s) 95
Sau3AI GATC 2 cut(s) 291, 463
SetI ASST 5 cut(s) 176, 229, 240, 395, 459
SfaNI GCATC 1 cut(s) 156
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
Sse9I AATT 3 cut(s) 269, 361, 379
SseBI AGGCCT 1 cut(s) 255
SspMI CTAG 3 cut(s) 239, 317, 353
StuI AGGCCT 1 cut(s) 255
TaaI ACNGT 1 cut(s) 453
TaiI ACGT 1 cut(s) 459
TaqI TCGA 2 cut(s) 290, 365
TasI AATT 3 cut(s) 269, 361, 379
TatI WGTACW 1 cut(s) 262
TfiI GAWTC 3 cut(s) 178, 205, 367
Tru1I TTAA 2 cut(s) 360, 481
Tru9I TTAA 2 cut(s) 360, 481
TscAI CASTG 1 cut(s) 428
TseI GCWGC 1 cut(s) 94
TspDTI ATGAA 1 cut(s) 422
TspRI CASTG 1 cut(s) 428
Tth111I GACNNNGTC 1 cut(s) 239
XapI RAATTY 2 cut(s) 269, 379
XceI RCATGY 1 cut(s) 201
XspI CTAG 3 cut(s) 239, 317, 353
Zsp2I ATGCAT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.