MD09G1236500.v1.1

2-aminoethanethiol dioxygenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004451
Physical Location & Seq
Forward (+)
29825101 .. 29834372
9272 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1236500.v1.1.491

Sequence Viewer

Length: 720 bp
ATGCCATATTATATTCAGAGACTGTATAATGTTTGCAAATCATCATTTTCGCCGACTGGACCTGTGTCAGAAGAAGCCCTTGAAAGAGTTCGCCTCAAACTAGAGAAAATTAAGCCTGCAGATGTAGGTCTTGAACAGGAGGCACAAGTGGTGCGTAATTGGTCAGGTGCTATGCCTGAGCGTAATGGGAATCAGCAGTCACTGCCACCAATCAAATACCTGCATTTGCATGAGTGTGATAGCTTTTCTATAGGAATTTTTTGCATGCCGCCTTCCTCTATCATACCCCTTCATAATCATCCTGGAATGACTGTGTTGAGCAAGCTTATATATGGTACAATACGTGTTAATTCATATGACTGGGTTGATTTTCCTGGCCCTGCGGATCCATCAGAAGCAAGACCTGCAAAGCTGGTCAAAGATACTGACATGACCGCGCCTTGTCCGCCAACAGTTCTTTATCCGACAACAGGAGGCAATATCCATAGTTTTAGAGCTATAACTCCCTGCGCCATATTTGACATCCTAGCTCCGCCTTACTCGTCCGAAGATGGACGACACTGCAGTTATTACCGGAAGTCCTCAAGAAAAGATCTGCCTGGCGATCTTGAGCTTGATGGAGTTAAAGAGCCTCAAGTGACTTGGTTAGAAGAGTTTCAACCTCCCGAAAACTTTGTGATTCGCCGAGGGCTGTACAAGGGCCCTGTCATTAGAACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008152 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00430 ko01100 map00430 map01100
Pfam Domains
Protein Families
PCO_ADO

Protein Analysis

240

Amino Acids

26.86

Weight (kDa)

6.59

Isoelectric Point (pI)

52.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 34 - 237 5.7e-77 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 228, 412
AccII CGCG 1 cut(s) 437
AciI CCGC 5 cut(s) 269, 383, 435, 446, 533
AclWI GGATC 2 cut(s) 380, 393
AcsI RAATTY 1 cut(s) 255
AfaI GTAC 2 cut(s) 337, 695
AfiI CCNNNNNNNGG 1 cut(s) 470
AflIII ACRYGT 1 cut(s) 343
AgsI TTSAA 3 cut(s) 83, 134, 659
AjnI CCWGG 3 cut(s) 301, 373, 598
AluBI AGCT 6 cut(s) 243, 325, 412, 497, 530, 613
AluI AGCT 6 cut(s) 243, 325, 412, 497, 530, 613
Alw26I GTCTC 1 cut(s) 13
AlwI GGATC 2 cut(s) 380, 393
AlwNI CAGNNNCTG 2 cut(s) 22, 202
AoxI GGCC 2 cut(s) 376, 700
ApaI GGGCCC 1 cut(s) 704
ApoI RAATTY 1 cut(s) 255
Asp700I GAANNNNTTC 2 cut(s) 87, 654
AspLEI GCGC 2 cut(s) 439, 512
AspS9I GGNCC 4 cut(s) 59, 377, 700, 701
AvaII GGWCC 1 cut(s) 59
BaeGI GKGCMC 1 cut(s) 704
BamHI GGATCC 1 cut(s) 385
BanII GRGCYC 1 cut(s) 704
BccI CCATC 3 cut(s) 397, 545, 611
BciT130I CCWGG 3 cut(s) 303, 375, 600
BcoDI GTCTC 1 cut(s) 13
BfaI CTAG 2 cut(s) 101, 527
BfmI CTRYAG 3 cut(s) 117, 249, 562
BfuAI ACCTGC 2 cut(s) 228, 412
BglII AGATCT 1 cut(s) 592
BisI GCNGC 1 cut(s) 269
BlsI GCNGC 1 cut(s) 270
Bme1390I CCNGG 3 cut(s) 303, 375, 600
Bme18I GGWCC 1 cut(s) 59
BmgT120I GGNCC 4 cut(s) 59, 377, 700, 701
BmiI GGNNCC 2 cut(s) 387, 702
BmrFI CCNGG 3 cut(s) 303, 375, 600
BmrI ACTGGG 1 cut(s) 370
BmuI ACTGGG 1 cut(s) 370
BoxI GACNNNNGTC 1 cut(s) 64
BplI GAGNNNNNCTC 2 cut(s) 78, 110
Bpu10I CCTNAGC 1 cut(s) 177
BpuEI CTTGAG 3 cut(s) 568, 618, 629
BsaAI YACGTR 1 cut(s) 344
BsaJI CCNNGG 1 cut(s) 685
BsaWI WCCGGW 1 cut(s) 573
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 2 cut(s) 61, 365
BseBI CCWGG 3 cut(s) 303, 375, 600
BseDI CCNNGG 1 cut(s) 685
BseGI GGATG 2 cut(s) 298, 522
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 2 cut(s) 61, 365
BseSI GKGCMC 1 cut(s) 704
Bsh1236I CGCG 1 cut(s) 437
BshFI GGCC 2 cut(s) 378, 702
BsiSI CCGG 1 cut(s) 574
BslI CCNNNNNNNGG 1 cut(s) 470
BsmAI GTCTC 1 cut(s) 13
BsnI GGCC 2 cut(s) 378, 702
Bsp120I GGGCCC 1 cut(s) 700
Bsp1286I GDGCHC 1 cut(s) 704
Bsp1407I TGTACA 1 cut(s) 693
Bsp143I GATC 3 cut(s) 385, 592, 604
BspACI CCGC 5 cut(s) 269, 383, 435, 446, 533
BspANI GGCC 2 cut(s) 378, 702
BspCNI CTCAG 1 cut(s) 169
BspFNI CGCG 1 cut(s) 437
BspLI GGNNCC 2 cut(s) 387, 702
BspMAI CTGCAG 2 cut(s) 121, 566
BspMI ACCTGC 2 cut(s) 228, 412
BspPI GGATC 2 cut(s) 380, 393
BsrGI TGTACA 1 cut(s) 693
BsrI ACTGG 2 cut(s) 61, 365
BssECI CCNNGG 1 cut(s) 685
BssMI GATC 3 cut(s) 385, 592, 604
Bst2UI CCWGG 3 cut(s) 303, 375, 600
Bst4CI ACNGT 3 cut(s) 24, 313, 454
Bst6I CTCTTC 1 cut(s) 645
BstAPI GCANNNNNTGC 2 cut(s) 202, 404
BstAUI TGTACA 1 cut(s) 693
BstBAI YACGTR 1 cut(s) 344
BstC8I GCNNGC 3 cut(s) 117, 266, 323
BstDEI CTNAG 1 cut(s) 177
BstF5I GGATG 2 cut(s) 298, 522
BstFNI CGCG 1 cut(s) 437
BstHHI GCGC 2 cut(s) 439, 512
BstKTI GATC 3 cut(s) 388, 595, 607
BstMAI GTCTC 1 cut(s) 13
BstMBI GATC 3 cut(s) 385, 592, 604
BstMWI GCNNNNNNNGC 3 cut(s) 202, 404, 445
BstNI CCWGG 3 cut(s) 303, 375, 600
BstNSI RCATGY 1 cut(s) 268
BstPAI GACNNNNGTC 1 cut(s) 64
BstSCI CCNGG 3 cut(s) 301, 373, 598
BstSFI CTRYAG 3 cut(s) 117, 249, 562
BstSLI GKGCMC 1 cut(s) 704
BstUI CGCG 1 cut(s) 437
BstX2I RGATCY 2 cut(s) 385, 592
BstYI RGATCY 2 cut(s) 385, 592
BsuRI GGCC 2 cut(s) 378, 702
BtsCI GGATG 2 cut(s) 298, 522
BtsI GCAGTG 2 cut(s) 200, 559
BtsIMutI CAGTG 2 cut(s) 200, 559
BveI ACCTGC 2 cut(s) 228, 412
Cac8I GCNNGC 3 cut(s) 117, 266, 323
CaiI CAGNNNCTG 2 cut(s) 22, 202
CfoI GCGC 2 cut(s) 439, 512
Cfr13I GGNCC 4 cut(s) 59, 377, 700, 701
Csp6I GTAC 2 cut(s) 336, 694
CviAII CATG 3 cut(s) 230, 265, 430
CviQI GTAC 2 cut(s) 336, 694
DdeI CTNAG 1 cut(s) 177
DpnI GATC 3 cut(s) 387, 594, 606
DpnII GATC 3 cut(s) 385, 592, 604
Eam1104I CTCTTC 1 cut(s) 645
EarI CTCTTC 1 cut(s) 645
EciI GGCGGA 2 cut(s) 435, 522
Eco24I GRGCYC 1 cut(s) 704
Eco47I GGWCC 1 cut(s) 59
EcoO109I RGGNCCY 2 cut(s) 700, 701
EcoRII CCWGG 3 cut(s) 301, 373, 598
EcoT38I GRGCYC 1 cut(s) 704
FaeI CATG 3 cut(s) 233, 268, 433
FalI AAGNNNNNCTT 2 cut(s) 63, 95
FatI CATG 3 cut(s) 229, 264, 429
FauNDI CATATG 1 cut(s) 355
Fnu4HI GCNGC 1 cut(s) 269
FokI GGATG 2 cut(s) 285, 509
FriOI GRGCYC 1 cut(s) 704
Fsp4HI GCNGC 1 cut(s) 269
FspBI CTAG 2 cut(s) 101, 527
GlaI GCGC 2 cut(s) 438, 511
GluI GCNGC 1 cut(s) 269
HaeIII GGCC 2 cut(s) 378, 702
HapII CCGG 1 cut(s) 574
HhaI GCGC 2 cut(s) 439, 512
Hin1II CATG 3 cut(s) 233, 268, 433
Hin6I GCGC 2 cut(s) 437, 510
HinP1I GCGC 2 cut(s) 437, 510
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 2 cut(s) 190, 679
HpaII CCGG 1 cut(s) 574
Hpy188I TCNGA 5 cut(s) 18, 70, 394, 465, 547
Hpy188III TCNNGA 4 cut(s) 131, 585, 608, 665
HpyAV CCTTC 2 cut(s) 282, 299
HpyCH4III ACNGT 3 cut(s) 24, 313, 454
HpyCH4IV ACGT 2 cut(s) 343, 716
HpyCH4V TGCA 7 cut(s) 36, 119, 223, 229, 264, 407, 564
HpyF10VI GCNNNNNNNGC 3 cut(s) 202, 404, 445
HpyF3I CTNAG 1 cut(s) 177
HpySE526I ACGT 2 cut(s) 343, 716
Hsp92II CATG 3 cut(s) 233, 268, 433
HspAI GCGC 2 cut(s) 437, 510
Kzo9I GATC 3 cut(s) 385, 592, 604
LmnI GCTCC 1 cut(s) 535
MaeI CTAG 2 cut(s) 101, 527
MaeII ACGT 2 cut(s) 343, 716
MaeIII GTNAC 2 cut(s) 198, 637
MalI GATC 3 cut(s) 387, 594, 606
MboI GATC 3 cut(s) 385, 592, 604
MboII GAAGA 3 cut(s) 83, 560, 662
MflI RGATCY 2 cut(s) 385, 592
MhlI GDGCHC 1 cut(s) 704
MluCI AATT 4 cut(s) 108, 157, 255, 349
MmeI TCCRAC 1 cut(s) 488
MnlI CCTC 8 cut(s) 104, 133, 286, 467, 592, 642, 672, 680
MroXI GAANNNNTTC 2 cut(s) 87, 654
MseI TTAA 3 cut(s) 111, 348, 624
MslI CAYNNNNRTG 2 cut(s) 228, 234
MspI CCGG 1 cut(s) 574
MspR9I CCNGG 3 cut(s) 303, 375, 600
MvaI CCWGG 3 cut(s) 303, 375, 600
MvnI CGCG 1 cut(s) 437
MwoI GCNNNNNNNGC 3 cut(s) 202, 404, 445
NdeI CATATG 1 cut(s) 355
NdeII GATC 3 cut(s) 385, 592, 604
NlaIII CATG 3 cut(s) 233, 268, 433
NlaIV GGNNCC 2 cut(s) 387, 702
NmeAIII GCCGAG 1 cut(s) 710
NmuCI GTSAC 2 cut(s) 198, 637
NspI RCATGY 1 cut(s) 268
PaeI GCATGC 1 cut(s) 268
PdmI GAANNNNTTC 2 cut(s) 87, 654
PfeI GAWTC 2 cut(s) 190, 679
PfoI TCCNGGA 1 cut(s) 301
PkrI GCNGC 1 cut(s) 270
Ppu21I YACGTR 1 cut(s) 344
PshAI GACNNNNGTC 1 cut(s) 64
Psp6I CCWGG 3 cut(s) 301, 373, 598
PspGI CCWGG 3 cut(s) 301, 373, 598
PspN4I GGNNCC 2 cut(s) 387, 702
PspOMI GGGCCC 1 cut(s) 700
PspPI GGNCC 4 cut(s) 59, 377, 700, 701
PstI CTGCAG 2 cut(s) 121, 566
PstNI CAGNNNCTG 2 cut(s) 22, 202
PsuI RGATCY 2 cut(s) 385, 592
RsaI GTAC 2 cut(s) 337, 695
RsaNI GTAC 2 cut(s) 336, 694
RseI CAYNNNNRTG 2 cut(s) 228, 234
SaqAI TTAA 3 cut(s) 111, 348, 624
SatI GCNGC 1 cut(s) 269
Sau3AI GATC 3 cut(s) 385, 592, 604
Sau96I GGNCC 4 cut(s) 59, 377, 700, 701
ScrFI CCNGG 3 cut(s) 303, 375, 600
SduI GDGCHC 1 cut(s) 704
SfcI CTRYAG 3 cut(s) 117, 249, 562
SinI GGWCC 1 cut(s) 59
SmiMI CAYNNNNRTG 2 cut(s) 228, 234
SmlI CTYRAG 3 cut(s) 583, 608, 633
SmoI CTYRAG 3 cut(s) 583, 608, 633
SphI GCATGC 1 cut(s) 268
Sse9I AATT 4 cut(s) 108, 157, 255, 349
SsiI CCGC 5 cut(s) 269, 383, 435, 446, 533
SspMI CTAG 2 cut(s) 101, 527
StyD4I CCNGG 3 cut(s) 301, 373, 598
TaaI ACNGT 3 cut(s) 24, 313, 454
TaiI ACGT 2 cut(s) 346, 719
TasI AATT 4 cut(s) 108, 157, 255, 349
TatI WGTACW 1 cut(s) 693
TauI GCSGC 1 cut(s) 271
TfiI GAWTC 2 cut(s) 190, 679
Tru1I TTAA 3 cut(s) 111, 348, 624
Tru9I TTAA 3 cut(s) 111, 348, 624
TscAI CASTG 2 cut(s) 207, 566
TseFI GTSAC 2 cut(s) 198, 637
Tsp45I GTSAC 2 cut(s) 198, 637
TspDTI ATGAA 2 cut(s) 281, 342
TspRI CASTG 2 cut(s) 207, 566
VpaK11BI GGWCC 1 cut(s) 59
XapI RAATTY 1 cut(s) 255
XceI RCATGY 1 cut(s) 268
XmnI GAANNNNTTC 2 cut(s) 87, 654
XspI CTAG 2 cut(s) 101, 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.