Rw2G027720

2-aminoethanethiol dioxygenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004451
Physical Location & Seq
Forward (+)
43792603 .. 43800552
7950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G027720.1

Sequence Viewer

Length: 720 bp
ATGCCATATTATATTCAGAGACTCTATAATCTGTGCAAATCATCTCTTTCGCCCAATGGACCCCTTTCCGAAGAAGCTCTCGAAAAAGTTCGCACTAAACTAGAGAAAATTAAGCCTGCAGATGTAGGTCTAGAACAAGAGGCACAAGTGGTACGCAACTGGTCAGGTGCTATGCTTGAGCGTAATGTGGATCATCAGTCACTGCCACCAATCAAATACTTGCATTTACATGAATGCGATAGTTTCTCTATGGGAATCTTTTGCATGCCGCCTTCCTCTATCATACCTCTTCATAATCATCCAGGAATGACTGTGTTGAGCAAGCTTATATATGGTACTTTGCGTGTTAATTCATATGACTGGCATGATTTACCCGGGTCTGCTGATCCATCAGAAGCAAGACCGGCGAAACTGGTCAGAGATGATGAGATGACCGCTCCTTGCCCGGCAACAGTTCTCTATCCCACAAGTGGAGGCAACATCCATAGTTTCAAAGCTATAACTCCCTGTGCCATATTTGACATTCTAGCTCCACCTTATTCGTCTGAAGATGGTCGACACTGCACTTATTTCCGGAAGTCCTCAAGAAAAGATCTGCCTGGTGATGTTGAGTATGATGGAGTCACAGCTCCTCAAGTGACTTGGTTAGAAGAGTTTCAACCTCCCGACAACTTTGTGATTCGGAGAGGGCTCTACAGGGGTCCTGTTATTCGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008152 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00430 ko01100 map00430 map01100
Pfam Domains
Protein Families
PCO_ADO

Protein Analysis

239

Amino Acids

26.86

Weight (kDa)

6.24

Isoelectric Point (pI)

52.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 34 - 237 1.5e-75 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 437
AccI GTMKAC 1 cut(s) 556
AccIII TCCGGA 1 cut(s) 573
AciI CCGC 2 cut(s) 269, 435
AclWI GGATC 2 cut(s) 198, 380
AcuI CTGAAG 1 cut(s) 567
AfaI GTAC 2 cut(s) 153, 337
AfiI CCNNNNNNNGG 1 cut(s) 470
AgsI TTSAA 2 cut(s) 493, 659
AjnI CCWGG 2 cut(s) 301, 598
AluBI AGCT 5 cut(s) 77, 325, 497, 530, 629
AluI AGCT 5 cut(s) 77, 325, 497, 530, 629
Alw26I GTCTC 1 cut(s) 13
AlwI GGATC 2 cut(s) 198, 380
AlwNI CAGNNNCTG 1 cut(s) 202
Ama87I CYCGRG 1 cut(s) 374
Aor13HI TCCGGA 1 cut(s) 573
Asp700I GAANNNNTTC 2 cut(s) 87, 654
AspS9I GGNCC 2 cut(s) 59, 701
AsuC2I CCSGG 3 cut(s) 375, 376, 446
AsuHPI GGTGA 1 cut(s) 614
AsuII TTCGAA 1 cut(s) 712
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 2 cut(s) 59, 701
BanII GRGCYC 1 cut(s) 693
BccI CCATC 3 cut(s) 397, 545, 611
BciT130I CCWGG 2 cut(s) 303, 600
BcnI CCSGG 3 cut(s) 375, 376, 446
BcoDI GTCTC 1 cut(s) 13
BfaI CTAG 3 cut(s) 101, 131, 527
BfmI CTRYAG 2 cut(s) 117, 694
BglII AGATCT 1 cut(s) 592
BisI GCNGC 1 cut(s) 269
BlsI GCNGC 1 cut(s) 270
Bme1390I CCNGG 5 cut(s) 303, 375, 376, 446, 600
Bme18I GGWCC 2 cut(s) 59, 701
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 2 cut(s) 59, 701
BmiI GGNNCC 2 cut(s) 61, 702
BmrFI CCNGG 5 cut(s) 303, 375, 376, 446, 600
Bpu14I TTCGAA 1 cut(s) 712
BpuEI CTTGAG 3 cut(s) 197, 568, 618
BpuMI CCSGG 3 cut(s) 375, 376, 446
BsaJI CCNNGG 1 cut(s) 374
BsaWI WCCGGW 1 cut(s) 573
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse118I RCCGGY 1 cut(s) 403
Bse1I ACTGG 3 cut(s) 164, 365, 417
BseAI TCCGGA 1 cut(s) 573
BseBI CCWGG 2 cut(s) 303, 600
BseDI CCNNGG 1 cut(s) 374
BseGI GGATG 2 cut(s) 298, 480
BseLI CCNNNNNNNGG 1 cut(s) 470
BseNI ACTGG 3 cut(s) 164, 365, 417
BseRI GAGGAG 1 cut(s) 621
BsgI GTGCAG 1 cut(s) 547
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 4 cut(s) 375, 404, 446, 574
BslI CCNNNNNNNGG 1 cut(s) 470
BsmAI GTCTC 1 cut(s) 13
BsmI GAATGC 1 cut(s) 239
BsoBI CYCGRG 1 cut(s) 374
Bsp119I TTCGAA 1 cut(s) 712
Bsp1286I GDGCHC 1 cut(s) 693
Bsp13I TCCGGA 1 cut(s) 573
Bsp143I GATC 3 cut(s) 190, 385, 592
BspACI CCGC 2 cut(s) 269, 435
BspEI TCCGGA 1 cut(s) 573
BspLI GGNNCC 2 cut(s) 61, 702
BspMAI CTGCAG 1 cut(s) 121
BspPI GGATC 2 cut(s) 198, 380
BspT104I TTCGAA 1 cut(s) 712
BsrBI CCGCTC 1 cut(s) 437
BsrFI RCCGGY 1 cut(s) 403
BsrI ACTGG 3 cut(s) 164, 365, 417
BssAI RCCGGY 1 cut(s) 403
BssECI CCNNGG 1 cut(s) 374
BssMI GATC 3 cut(s) 190, 385, 592
Bst2UI CCWGG 2 cut(s) 303, 600
Bst4CI ACNGT 2 cut(s) 313, 454
Bst6I CTCTTC 2 cut(s) 294, 645
BstBI TTCGAA 1 cut(s) 712
BstC8I GCNNGC 3 cut(s) 117, 266, 323
BstF5I GGATG 2 cut(s) 298, 480
BstKTI GATC 3 cut(s) 193, 388, 595
BstMAI GTCTC 1 cut(s) 13
BstMBI GATC 3 cut(s) 190, 385, 592
BstMWI GCNNNNNNNGC 1 cut(s) 404
BstNI CCWGG 2 cut(s) 303, 600
BstNSI RCATGY 1 cut(s) 268
BstSCI CCNGG 5 cut(s) 301, 373, 374, 444, 598
BstSFI CTRYAG 2 cut(s) 117, 694
BstX2I RGATCY 1 cut(s) 592
BstYI RGATCY 1 cut(s) 592
BtsCI GGATG 2 cut(s) 298, 480
BtsI GCAGTG 2 cut(s) 200, 559
BtsIMutI CAGTG 2 cut(s) 200, 559
Cac8I GCNNGC 3 cut(s) 117, 266, 323
CaiI CAGNNNCTG 1 cut(s) 202
Cfr10I RCCGGY 1 cut(s) 403
Cfr13I GGNCC 2 cut(s) 59, 701
Cfr9I CCCGGG 1 cut(s) 374
Csp6I GTAC 2 cut(s) 152, 336
CviAII CATG 3 cut(s) 230, 265, 365
CviJI RGCY 7 cut(s) 77, 115, 325, 497, 530, 629, 691
CviKI_1 RGCY 7 cut(s) 77, 115, 325, 497, 530, 629, 691
CviQI GTAC 2 cut(s) 152, 336
DpnI GATC 3 cut(s) 192, 387, 594
DpnII GATC 3 cut(s) 190, 385, 592
Eam1104I CTCTTC 2 cut(s) 294, 645
EarI CTCTTC 2 cut(s) 294, 645
Eco24I GRGCYC 1 cut(s) 693
Eco47I GGWCC 2 cut(s) 59, 701
Eco57I CTGAAG 1 cut(s) 567
Eco88I CYCGRG 1 cut(s) 374
EcoO109I RGGNCCY 1 cut(s) 701
EcoRII CCWGG 2 cut(s) 301, 598
EcoT38I GRGCYC 1 cut(s) 693
FaeI CATG 3 cut(s) 233, 268, 368
FatI CATG 3 cut(s) 229, 264, 364
FauNDI CATATG 1 cut(s) 355
FblI GTMKAC 1 cut(s) 556
Fnu4HI GCNGC 1 cut(s) 269
FokI GGATG 2 cut(s) 285, 467
FriOI GRGCYC 1 cut(s) 693
Fsp4HI GCNGC 1 cut(s) 269
FspBI CTAG 3 cut(s) 101, 131, 527
GluI GCNGC 1 cut(s) 269
HapII CCGG 4 cut(s) 375, 404, 446, 574
Hin1II CATG 3 cut(s) 233, 268, 368
HincII GTYRAC 1 cut(s) 557
HindII GTYRAC 1 cut(s) 557
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 4 cut(s) 21, 255, 621, 679
HpaII CCGG 4 cut(s) 375, 404, 446, 574
HphI GGTGA 1 cut(s) 614
Hpy166II GTNNAC 1 cut(s) 557
Hpy188I TCNGA 6 cut(s) 18, 70, 394, 419, 547, 684
Hpy188III TCNNGA 5 cut(s) 80, 131, 574, 585, 665
Hpy8I GTNNAC 1 cut(s) 557
HpyAV CCTTC 1 cut(s) 282
HpyCH4III ACNGT 2 cut(s) 313, 454
HpyCH4V TGCA 5 cut(s) 36, 119, 223, 264, 564
HpyF10VI GCNNNNNNNGC 1 cut(s) 404
Hsp92II CATG 3 cut(s) 233, 268, 368
Kpn2I TCCGGA 1 cut(s) 573
Kzo9I GATC 3 cut(s) 190, 385, 592
LmnI GCTCC 3 cut(s) 442, 535, 634
MaeI CTAG 3 cut(s) 101, 131, 527
MaeIII GTNAC 3 cut(s) 198, 622, 637
MalI GATC 3 cut(s) 192, 387, 594
MbiI CCGCTC 1 cut(s) 437
MboI GATC 3 cut(s) 190, 385, 592
MboII GAAGA 4 cut(s) 83, 281, 560, 662
MflI RGATCY 1 cut(s) 592
MhlI GDGCHC 1 cut(s) 693
MluCI AATT 2 cut(s) 108, 349
MlyI GAGTC 2 cut(s) 15, 630
MnlI CCTC 8 cut(s) 133, 286, 297, 467, 592, 642, 672, 680
MroI TCCGGA 1 cut(s) 573
MroXI GAANNNNTTC 2 cut(s) 87, 654
MseI TTAA 2 cut(s) 111, 348
MslI CAYNNNNRTG 1 cut(s) 228
MspI CCGG 4 cut(s) 375, 404, 446, 574
MspR9I CCNGG 5 cut(s) 303, 375, 376, 446, 600
Mva1269I GAATGC 1 cut(s) 239
MvaI CCWGG 2 cut(s) 303, 600
MwoI GCNNNNNNNGC 1 cut(s) 404
NciI CCSGG 3 cut(s) 375, 376, 446
NdeI CATATG 1 cut(s) 355
NdeII GATC 3 cut(s) 190, 385, 592
NlaIII CATG 3 cut(s) 233, 268, 368
NlaIV GGNNCC 2 cut(s) 61, 702
NmuCI GTSAC 3 cut(s) 198, 622, 637
NspI RCATGY 1 cut(s) 268
NspV TTCGAA 1 cut(s) 712
PaeI GCATGC 1 cut(s) 268
PctI GAATGC 1 cut(s) 239
PdmI GAANNNNTTC 2 cut(s) 87, 654
PfeI GAWTC 2 cut(s) 255, 679
PfoI TCCNGGA 1 cut(s) 301
PkrI GCNGC 1 cut(s) 270
PleI GAGTC 2 cut(s) 15, 629
PpsI GAGTC 2 cut(s) 15, 629
PpuMI RGGWCCY 1 cut(s) 701
Psp5II RGGWCCY 1 cut(s) 701
Psp6I CCWGG 2 cut(s) 301, 598
PspGI CCWGG 2 cut(s) 301, 598
PspN4I GGNNCC 2 cut(s) 61, 702
PspPI GGNCC 2 cut(s) 59, 701
PspPPI RGGWCCY 1 cut(s) 701
PstI CTGCAG 1 cut(s) 121
PstNI CAGNNNCTG 1 cut(s) 202
PsuI RGATCY 1 cut(s) 592
RsaI GTAC 2 cut(s) 153, 337
RsaNI GTAC 2 cut(s) 152, 336
RseI CAYNNNNRTG 1 cut(s) 228
SalI GTCGAC 1 cut(s) 555
SaqAI TTAA 2 cut(s) 111, 348
SatI GCNGC 1 cut(s) 269
Sau3AI GATC 3 cut(s) 190, 385, 592
Sau96I GGNCC 2 cut(s) 59, 701
SchI GAGTC 2 cut(s) 15, 630
ScrFI CCNGG 5 cut(s) 303, 375, 376, 446, 600
SduI GDGCHC 1 cut(s) 693
SfcI CTRYAG 2 cut(s) 117, 694
SfuI TTCGAA 1 cut(s) 712
SinI GGWCC 2 cut(s) 59, 701
SmaI CCCGGG 1 cut(s) 376
SmiMI CAYNNNNRTG 1 cut(s) 228
SmlI CTYRAG 3 cut(s) 176, 583, 633
SmoI CTYRAG 3 cut(s) 176, 583, 633
SphI GCATGC 1 cut(s) 268
Sse9I AATT 2 cut(s) 108, 349
SsiI CCGC 2 cut(s) 269, 435
SspMI CTAG 3 cut(s) 101, 131, 527
StyD4I CCNGG 5 cut(s) 301, 373, 374, 444, 598
TaaI ACNGT 2 cut(s) 313, 454
TaqI TCGA 3 cut(s) 81, 556, 712
TasI AATT 2 cut(s) 108, 349
TauI GCSGC 1 cut(s) 271
TfiI GAWTC 2 cut(s) 255, 679
Tru1I TTAA 2 cut(s) 111, 348
Tru9I TTAA 2 cut(s) 111, 348
TscAI CASTG 2 cut(s) 207, 566
TseFI GTSAC 3 cut(s) 198, 622, 637
Tsp45I GTSAC 3 cut(s) 198, 622, 637
TspDTI ATGAA 3 cut(s) 246, 281, 342
TspMI CCCGGG 1 cut(s) 374
TspRI CASTG 2 cut(s) 207, 566
VpaK11BI GGWCC 2 cut(s) 59, 701
XbaI TCTAGA 1 cut(s) 130
XceI RCATGY 1 cut(s) 268
XmaI CCCGGG 1 cut(s) 374
XmiI GTMKAC 1 cut(s) 556
XmnI GAANNNNTTC 2 cut(s) 87, 654
XspI CTAG 3 cut(s) 101, 131, 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.