MD17G1266100.v1.1

2-aminoethanethiol dioxygenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004451
Physical Location & Seq
Forward (+)
32665837 .. 32670710
4874 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1266100.v1.1.491

Sequence Viewer

Length: 720 bp
ATGCCATATTATATTCAGAGACTCTATAATGTATGCAAATCTTCATATACGCCGACCGGACCTTTGTCAGAAGAAGCCCTGGAAAGAGTTCGTGTCAAACTAGAGAAAATTAAGCCTGCAGATGTAGGTCTCGAACAGGAGGCACAAGTGGTGCGTAATTGGTCAGGTGCTATGCCTGAGCATAATGGGATTCAGCAGTCACTTCCACCAATCAAATACTTGCATTTACATGAGTGTGGTAGCTTTTCTATAGGAATTTTTTGCATGCCGCCTTCCTCTATCATACCTCTTCATAATCATCCTGGAATGACTGTGTTGAGCAAGCTTCTATATGGTACATTACGTGTTAATTCATATGACTGGGTTGATTTTCCTGGCCCTGCCGATCCATCAGAAGCGAGACCTGCAAAGCTGGTCAAAGACACTGACATGACCGCGCCCTGTCCACCAACAGTTCTTTACCCCACAACTGGAGGCAATATCCATAGCTTTAGAGCTATAACTCCCTGCGCCATCTTTGACATCCTAGCTCCGCCTTACTCTTCTGAAGAAGGACGACACTGCAGTTATTACCGGAAGTCCTCAAGAAGAGATCTGCCTGCTGATCTTGAGCTCGATGGAGTTATAGAGCCTCAAGTGACTTGGCTAGAAGAGTTTCAACCTCCCGAAAACTTTGTGATTCGCAGAGGGCTGTACAAGGGCCCCGTTATTAGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008152 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00430 ko01100 map00430 map01100
Pfam Domains
Protein Families
PCO_ADO

Protein Analysis

240

Amino Acids

26.85

Weight (kDa)

6.38

Isoelectric Point (pI)

55.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 34 - 237 2.2e-75 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 412
AccII CGCG 1 cut(s) 437
AciI CCGC 3 cut(s) 269, 435, 533
AclWI GGATC 1 cut(s) 380
AcsI RAATTY 1 cut(s) 255
AcuI CTGAAG 1 cut(s) 567
AfaI GTAC 2 cut(s) 337, 695
AfiI CCNNNNNNNGG 1 cut(s) 470
AflIII ACRYGT 1 cut(s) 343
AgsI TTSAA 1 cut(s) 659
AjnI CCWGG 3 cut(s) 78, 301, 373
AluBI AGCT 7 cut(s) 243, 325, 412, 489, 497, 530, 613
AluI AGCT 7 cut(s) 243, 325, 412, 489, 497, 530, 613
Alw21I GWGCWC 1 cut(s) 615
Alw26I GTCTC 3 cut(s) 13, 134, 394
AlwI GGATC 1 cut(s) 380
AoxI GGCC 2 cut(s) 376, 700
ApaI GGGCCC 1 cut(s) 704
ApoI RAATTY 1 cut(s) 255
ArsI GACNNNNNNTTYG 2 cut(s) 46, 78
Asp700I GAANNNNTTC 2 cut(s) 87, 654
AspLEI GCGC 2 cut(s) 439, 512
AspS9I GGNCC 4 cut(s) 59, 377, 700, 701
AvaII GGWCC 1 cut(s) 59
BaeGI GKGCMC 1 cut(s) 704
BanII GRGCYC 2 cut(s) 615, 704
Bbv12I GWGCWC 1 cut(s) 615
BccI CCATC 3 cut(s) 397, 521, 611
BciT130I CCWGG 3 cut(s) 80, 303, 375
BcoDI GTCTC 3 cut(s) 13, 134, 394
BfaI CTAG 3 cut(s) 101, 527, 647
BfmI CTRYAG 3 cut(s) 117, 249, 562
BfuAI ACCTGC 1 cut(s) 412
BglII AGATCT 1 cut(s) 592
BisI GCNGC 1 cut(s) 269
BlsI GCNGC 1 cut(s) 270
Bme1390I CCNGG 3 cut(s) 80, 303, 375
Bme18I GGWCC 1 cut(s) 59
BmgT120I GGNCC 4 cut(s) 59, 377, 700, 701
BmiI GGNNCC 2 cut(s) 702, 703
BmrFI CCNGG 3 cut(s) 80, 303, 375
BmrI ACTGGG 1 cut(s) 370
BmuI ACTGGG 1 cut(s) 370
BoxI GACNNNNGTC 1 cut(s) 64
BpmI CTGGAG 1 cut(s) 492
Bpu10I CCTNAGC 1 cut(s) 177
BpuEI CTTGAG 3 cut(s) 568, 618, 629
BsaAI YACGTR 1 cut(s) 344
BsaI GGTCTC 2 cut(s) 134, 394
BsaJI CCNNGG 1 cut(s) 78
BsaWI WCCGGW 2 cut(s) 56, 573
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 2 cut(s) 365, 475
BseBI CCWGG 3 cut(s) 80, 303, 375
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 2 cut(s) 298, 522
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 2 cut(s) 365, 475
BseSI GKGCMC 1 cut(s) 704
Bsh1236I CGCG 1 cut(s) 437
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 2 cut(s) 378, 702
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 1 cut(s) 615
BsiSI CCGG 2 cut(s) 57, 574
BslI CCNNNNNNNGG 1 cut(s) 470
BsmAI GTCTC 3 cut(s) 13, 134, 394
BsnI GGCC 2 cut(s) 378, 702
Bso31I GGTCTC 2 cut(s) 134, 394
Bsp120I GGGCCC 1 cut(s) 700
Bsp1286I GDGCHC 2 cut(s) 615, 704
Bsp1407I TGTACA 1 cut(s) 693
Bsp143I GATC 3 cut(s) 385, 592, 604
BspACI CCGC 3 cut(s) 269, 435, 533
BspANI GGCC 2 cut(s) 378, 702
BspCNI CTCAG 1 cut(s) 169
BspFNI CGCG 1 cut(s) 437
BspLI GGNNCC 2 cut(s) 702, 703
BspMAI CTGCAG 2 cut(s) 121, 566
BspMI ACCTGC 1 cut(s) 412
BspPI GGATC 1 cut(s) 380
BspTNI GGTCTC 2 cut(s) 134, 394
BsrGI TGTACA 1 cut(s) 693
BsrI ACTGG 2 cut(s) 365, 475
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 3 cut(s) 385, 592, 604
Bst2UI CCWGG 3 cut(s) 80, 303, 375
Bst4CI ACNGT 2 cut(s) 313, 454
Bst6I CTCTTC 4 cut(s) 294, 547, 583, 645
BstAUI TGTACA 1 cut(s) 693
BstBAI YACGTR 1 cut(s) 344
BstC8I GCNNGC 4 cut(s) 117, 266, 323, 600
BstDEI CTNAG 1 cut(s) 177
BstF5I GGATG 2 cut(s) 298, 522
BstFNI CGCG 1 cut(s) 437
BstHHI GCGC 2 cut(s) 439, 512
BstKTI GATC 3 cut(s) 388, 595, 607
BstMAI GTCTC 3 cut(s) 13, 134, 394
BstMBI GATC 3 cut(s) 385, 592, 604
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 1 cut(s) 404
BstNI CCWGG 3 cut(s) 80, 303, 375
BstNSI RCATGY 1 cut(s) 268
BstPAI GACNNNNGTC 1 cut(s) 64
BstSCI CCNGG 3 cut(s) 78, 301, 373
BstSFI CTRYAG 3 cut(s) 117, 249, 562
BstSLI GKGCMC 1 cut(s) 704
BstUI CGCG 1 cut(s) 437
BstX2I RGATCY 1 cut(s) 592
BstYI RGATCY 1 cut(s) 592
BsuRI GGCC 2 cut(s) 378, 702
BtsCI GGATG 2 cut(s) 298, 522
BtsI GCAGTG 1 cut(s) 559
BtsIMutI CAGTG 2 cut(s) 423, 559
BveI ACCTGC 1 cut(s) 412
Cac8I GCNNGC 4 cut(s) 117, 266, 323, 600
CfoI GCGC 2 cut(s) 439, 512
Cfr13I GGNCC 4 cut(s) 59, 377, 700, 701
Csp6I GTAC 2 cut(s) 336, 694
CviAII CATG 3 cut(s) 230, 265, 430
CviQI GTAC 2 cut(s) 336, 694
DdeI CTNAG 1 cut(s) 177
DpnI GATC 3 cut(s) 387, 594, 606
DpnII GATC 3 cut(s) 385, 592, 604
Eam1104I CTCTTC 4 cut(s) 294, 547, 583, 645
EarI CTCTTC 4 cut(s) 294, 547, 583, 645
EciI GGCGGA 1 cut(s) 522
Ecl136II GAGCTC 1 cut(s) 613
Eco24I GRGCYC 2 cut(s) 615, 704
Eco31I GGTCTC 2 cut(s) 134, 394
Eco47I GGWCC 1 cut(s) 59
Eco53kI GAGCTC 1 cut(s) 613
Eco57I CTGAAG 1 cut(s) 567
EcoICRI GAGCTC 1 cut(s) 613
EcoO109I RGGNCCY 2 cut(s) 700, 701
EcoRII CCWGG 3 cut(s) 78, 301, 373
EcoT38I GRGCYC 2 cut(s) 615, 704
FaeI CATG 3 cut(s) 233, 268, 433
FatI CATG 3 cut(s) 229, 264, 429
FauNDI CATATG 1 cut(s) 355
Fnu4HI GCNGC 1 cut(s) 269
FokI GGATG 2 cut(s) 285, 509
FriOI GRGCYC 2 cut(s) 615, 704
Fsp4HI GCNGC 1 cut(s) 269
FspBI CTAG 3 cut(s) 101, 527, 647
GlaI GCGC 2 cut(s) 438, 511
GluI GCNGC 1 cut(s) 269
GsuI CTGGAG 1 cut(s) 492
HaeIII GGCC 2 cut(s) 378, 702
HapII CCGG 2 cut(s) 57, 574
HhaI GCGC 2 cut(s) 439, 512
Hin1II CATG 3 cut(s) 233, 268, 433
Hin6I GCGC 2 cut(s) 437, 510
HinP1I GCGC 2 cut(s) 437, 510
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 3 cut(s) 21, 190, 679
HpaII CCGG 2 cut(s) 57, 574
Hpy166II GTNNAC 1 cut(s) 446
Hpy188I TCNGA 4 cut(s) 18, 70, 394, 547
Hpy188III TCNNGA 4 cut(s) 131, 585, 608, 665
Hpy8I GTNNAC 1 cut(s) 446
HpyAV CCTTC 2 cut(s) 282, 545
HpyCH4III ACNGT 2 cut(s) 313, 454
HpyCH4IV ACGT 1 cut(s) 343
HpyCH4V TGCA 6 cut(s) 36, 119, 223, 264, 407, 564
HpyF10VI GCNNNNNNNGC 1 cut(s) 404
HpyF3I CTNAG 1 cut(s) 177
HpySE526I ACGT 1 cut(s) 343
Hsp92II CATG 3 cut(s) 233, 268, 433
HspAI GCGC 2 cut(s) 437, 510
Kzo9I GATC 3 cut(s) 385, 592, 604
LmnI GCTCC 1 cut(s) 535
MaeI CTAG 3 cut(s) 101, 527, 647
MaeII ACGT 1 cut(s) 343
MaeIII GTNAC 2 cut(s) 198, 637
MalI GATC 3 cut(s) 387, 594, 606
MboI GATC 3 cut(s) 385, 592, 604
MboII GAAGA 7 cut(s) 33, 83, 281, 534, 560, 600, 662
MflI RGATCY 1 cut(s) 592
MhlI GDGCHC 2 cut(s) 615, 704
MluCI AATT 4 cut(s) 108, 157, 255, 349
MlyI GAGTC 1 cut(s) 15
MnlI CCTC 8 cut(s) 133, 286, 297, 467, 592, 642, 672, 680
MroXI GAANNNNTTC 2 cut(s) 87, 654
MseI TTAA 2 cut(s) 111, 348
MslI CAYNNNNRTG 3 cut(s) 228, 234, 428
MspI CCGG 2 cut(s) 57, 574
MspR9I CCNGG 3 cut(s) 80, 303, 375
MvaI CCWGG 3 cut(s) 80, 303, 375
MvnI CGCG 1 cut(s) 437
MwoI GCNNNNNNNGC 1 cut(s) 404
NdeI CATATG 1 cut(s) 355
NdeII GATC 3 cut(s) 385, 592, 604
NlaIII CATG 3 cut(s) 233, 268, 433
NlaIV GGNNCC 2 cut(s) 702, 703
NmuCI GTSAC 2 cut(s) 198, 637
NspI RCATGY 1 cut(s) 268
PaeI GCATGC 1 cut(s) 268
PdmI GAANNNNTTC 2 cut(s) 87, 654
PfeI GAWTC 2 cut(s) 190, 679
PfoI TCCNGGA 1 cut(s) 301
PkrI GCNGC 1 cut(s) 270
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
Ppu21I YACGTR 1 cut(s) 344
PshAI GACNNNNGTC 1 cut(s) 64
Psp124BI GAGCTC 1 cut(s) 615
Psp6I CCWGG 3 cut(s) 78, 301, 373
PspGI CCWGG 3 cut(s) 78, 301, 373
PspN4I GGNNCC 2 cut(s) 702, 703
PspOMI GGGCCC 1 cut(s) 700
PspPI GGNCC 4 cut(s) 59, 377, 700, 701
PstI CTGCAG 2 cut(s) 121, 566
PsuI RGATCY 1 cut(s) 592
RsaI GTAC 2 cut(s) 337, 695
RsaNI GTAC 2 cut(s) 336, 694
RseI CAYNNNNRTG 3 cut(s) 228, 234, 428
SacI GAGCTC 1 cut(s) 615
SaqAI TTAA 2 cut(s) 111, 348
SatI GCNGC 1 cut(s) 269
Sau3AI GATC 3 cut(s) 385, 592, 604
Sau96I GGNCC 4 cut(s) 59, 377, 700, 701
SchI GAGTC 1 cut(s) 15
ScrFI CCNGG 3 cut(s) 80, 303, 375
SduI GDGCHC 2 cut(s) 615, 704
SfcI CTRYAG 3 cut(s) 117, 249, 562
SinI GGWCC 1 cut(s) 59
SmiMI CAYNNNNRTG 3 cut(s) 228, 234, 428
SmlI CTYRAG 3 cut(s) 583, 608, 633
SmoI CTYRAG 3 cut(s) 583, 608, 633
SphI GCATGC 1 cut(s) 268
Sse9I AATT 4 cut(s) 108, 157, 255, 349
SsiI CCGC 3 cut(s) 269, 435, 533
SspMI CTAG 3 cut(s) 101, 527, 647
SstI GAGCTC 1 cut(s) 615
StyD4I CCNGG 3 cut(s) 78, 301, 373
TaaI ACNGT 2 cut(s) 313, 454
TaiI ACGT 1 cut(s) 346
TaqI TCGA 2 cut(s) 132, 615
TasI AATT 4 cut(s) 108, 157, 255, 349
TatI WGTACW 1 cut(s) 693
TauI GCSGC 1 cut(s) 271
TfiI GAWTC 2 cut(s) 190, 679
Tru1I TTAA 2 cut(s) 111, 348
Tru9I TTAA 2 cut(s) 111, 348
TscAI CASTG 2 cut(s) 430, 566
TseFI GTSAC 2 cut(s) 198, 637
Tsp45I GTSAC 2 cut(s) 198, 637
TspDTI ATGAA 3 cut(s) 33, 281, 342
TspRI CASTG 2 cut(s) 430, 566
VpaK11BI GGWCC 1 cut(s) 59
XapI RAATTY 1 cut(s) 255
XceI RCATGY 1 cut(s) 268
XmnI GAANNNNTTC 2 cut(s) 87, 654
XspI CTAG 3 cut(s) 101, 527, 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.