MD09G1265900.v1.1

Oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003907
Physical Location & Seq
Forward (+)
34051252 .. 34053119
1868 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1265900.v1.1.491

Sequence Viewer

Length: 1089 bp
ATGGCAGAAAGTCCAGCAGCAATACGTTTCGGCATAACAGGATGCGCCGACATAGCCAGAAAAGTAGCTCGAGCCATAAAGCTAGCACCCAACGCCACCCTCTACGCCATCGCTAGCCGTTCGATCGACAAAGCCCGGAAATTCGCAGCCCACAATGGACTCTCCGGAGCCGTCAAGATCTACGGAAGCTACGAGCAGCTGCTCGACGACCCATGTGTAGATGCGGTGTACATGCCCCTGCCCACAAGTCTTCACCTGCAATGGGCGGTGTTGGCTGCTCGGAAGAAGAAGCATTTGCTTTTGGAAAAACCAACGGCTCTTGATGTGGGAGAGCTTGATCGGATACTGGAAGCGTGCGAATCCAATGGTGTACAGTTCATGGATGGGAGCATGTGGCTGCATCATCCAAGGACTGCAAAATTGAAGGACTTGATCTTTGATCCCAAGCTTTTTGGTCAAACTGACTTTATCCGTAGCACGTCAACACTGCCAGGAACCCAGGATTTCTTGGAGAGCAACATCAGAGTGAAACCGAACTTGGATGCTCTCGGTGCACTTGGGGGCGTAGGCTGGTACTGTATTGGGGCCATCTTGTGGGCAAAGGACTACAAATTACCAACTGTGGTGAATGCTCTACCTGATGTCATAAGAAACTCTGCAGGAGTCATCTTGTCTTGCTCTGCATCATTTCATTGGGAACCCGCAGACAAAACCGTCGCCACAATTCACTGCTCTTTCCTCTCCCACACGTCCATGGACTTGGCTATGACTGGCTCTAATGGTTCGCTGCATTGCAATGACTACATTATTCCATACCAGGAGAATTCGGCTTCATTTGACTACATATCCGGGGCAACATTTGTGGAACTCCACATTGGGTGGAATGTAAAACCAGAAGAAGTTACCGTCACTTCTGAGCTGCCGCAAGAGGCATTGATGGTTCAAGAGTTTGCAAGGAATGCTGAGAACATTAGGAAATTTGGAAATCGTCCGGACAGTAAATGGCCCGATATCAGCAGAAAAACGCAGTTAGTATTGGATGCTGTGAAGAAATCCATTGATCTTGGTTGTAAACCTGTTAAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GFO_IDH_MocA

Protein Analysis

363

Amino Acids

39.76

Weight (kDa)

7.13

Isoelectric Point (pI)

30.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GFO_IDH_MocA PF01408 8 - 127 4.8e-19 Oxidoreductase family, NAD-binding Rossmann fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 264
AasI GACNNNNNNGTC 1 cut(s) 713
Acc36I ACCTGC 1 cut(s) 264
AccB7I CCANNNNNTGG 1 cut(s) 594
AccIII TCCGGA 2 cut(s) 164, 991
AciI CCGC 4 cut(s) 224, 266, 702, 923
AclWI GGATC 1 cut(s) 434
AcsI RAATTY 3 cut(s) 140, 823, 977
AfaI GTAC 3 cut(s) 230, 372, 575
AfiI CCNNNNNNNGG 2 cut(s) 262, 594
AflIII ACRYGT 1 cut(s) 747
AgsI TTSAA 2 cut(s) 424, 944
AjiI CACGTC 2 cut(s) 480, 750
AjnI CCWGG 3 cut(s) 490, 498, 816
AjuI GAANNNNNNNTTGG 4 cut(s) 521, 553, 858, 890
AluBI AGCT 7 cut(s) 68, 82, 189, 199, 334, 448, 919
AluI AGCT 7 cut(s) 68, 82, 189, 199, 334, 448, 919
Alw21I GWGCWC 1 cut(s) 556
Alw44I GTGCAC 1 cut(s) 552
AlwI GGATC 1 cut(s) 434
Ama87I CYCGRG 1 cut(s) 69
Aor13HI TCCGGA 2 cut(s) 164, 991
AoxI GGCC 2 cut(s) 585, 1004
ApaLI GTGCAC 1 cut(s) 552
ApeKI GCWGC 8 cut(s) 17, 146, 196, 199, 275, 397, 787, 919
ApoI RAATTY 3 cut(s) 140, 823, 977
ArsI GACNNNNNNTTYG 2 cut(s) 419, 451
AspLEI GCGC 1 cut(s) 47
AspS9I GGNCC 2 cut(s) 585, 1005
AsuC2I CCSGG 2 cut(s) 136, 850
AsuHPI GGTGA 2 cut(s) 245, 637
AsuNHI GCTAGC 2 cut(s) 82, 113
AvaI CYCGRG 1 cut(s) 69
BaeGI GKGCMC 1 cut(s) 556
BbsI GAAGAC 1 cut(s) 242
Bbv12I GWGCWC 1 cut(s) 556
BbvI GCAGC 8 cut(s) 29, 158, 186, 208, 262, 384, 774, 906
BccI CCATC 4 cut(s) 116, 377, 596, 931
BceAI ACGGC 3 cut(s) 102, 155, 330
BciT130I CCWGG 3 cut(s) 492, 500, 818
BciVI GTATCC 1 cut(s) 336
BcnI CCSGG 2 cut(s) 136, 850
BfaI CTAG 2 cut(s) 83, 114
BfmI CTRYAG 1 cut(s) 657
BfuAI ACCTGC 1 cut(s) 264
BfuI GTATCC 1 cut(s) 336
BglII AGATCT 1 cut(s) 177
BisI GCNGC 9 cut(s) 18, 147, 197, 200, 276, 398, 788, 920, 923
BlsI GCNGC 9 cut(s) 19, 148, 198, 201, 277, 399, 789, 921, 924
Bme1390I CCNGG 5 cut(s) 136, 492, 500, 818, 850
BmeT110I CYCGRG 1 cut(s) 69
BmgBI CACGTC 2 cut(s) 480, 750
BmgT120I GGNCC 2 cut(s) 585, 1005
BmiI GGNNCC 4 cut(s) 169, 496, 586, 699
BmrFI CCNGG 5 cut(s) 136, 492, 500, 818, 850
BmsI GCATC 6 cut(s) 32, 211, 409, 532, 692, 1030
BmtI GCTAGC 2 cut(s) 86, 117
BpiI GAAGAC 1 cut(s) 242
BpuMI CCSGG 2 cut(s) 136, 850
BsaJI CCNNGG 4 cut(s) 407, 498, 753, 849
BsaWI WCCGGW 2 cut(s) 164, 991
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 594
Bse1I ACTGG 2 cut(s) 351, 775
Bse3DI GCAATG 3 cut(s) 266, 790, 802
BseAI TCCGGA 2 cut(s) 164, 991
BseBI CCWGG 3 cut(s) 492, 500, 818
BseDI CCNNGG 4 cut(s) 407, 498, 753, 849
BseGI GGATG 5 cut(s) 47, 388, 403, 547, 1045
BseLI CCNNNNNNNGG 2 cut(s) 262, 594
BseMI GCAATG 3 cut(s) 266, 790, 802
BseMII CTCAG 2 cut(s) 906, 954
BseNI ACTGG 2 cut(s) 351, 775
BseSI GKGCMC 1 cut(s) 556
BseXI GCAGC 8 cut(s) 29, 158, 186, 208, 262, 384, 774, 906
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 2 cut(s) 587, 1006
BsiEI CGRYCG 1 cut(s) 126
BsiHKAI GWGCWC 1 cut(s) 556
BsiHKCI CYCGRG 1 cut(s) 69
BsiSI CCGG 4 cut(s) 136, 165, 849, 992
BslI CCNNNNNNNGG 2 cut(s) 262, 594
BsmI GAATGC 2 cut(s) 634, 964
BsnI GGCC 2 cut(s) 587, 1006
BsoBI CYCGRG 1 cut(s) 69
Bsp1286I GDGCHC 1 cut(s) 556
Bsp13I TCCGGA 2 cut(s) 164, 991
Bsp1407I TGTACA 2 cut(s) 228, 370
Bsp143I GATC 6 cut(s) 123, 177, 337, 432, 439, 1060
Bsp19I CCATGG 1 cut(s) 753
BspACI CCGC 4 cut(s) 224, 266, 702, 923
BspANI GGCC 2 cut(s) 587, 1006
BspCNI CTCAG 2 cut(s) 907, 955
BspEI TCCGGA 2 cut(s) 164, 991
BspLI GGNNCC 4 cut(s) 169, 496, 586, 699
BspMAI CTGCAG 1 cut(s) 661
BspMI ACCTGC 1 cut(s) 264
BspOI GCTAGC 2 cut(s) 86, 117
BspPI GGATC 1 cut(s) 434
BsrDI GCAATG 3 cut(s) 266, 790, 802
BsrGI TGTACA 2 cut(s) 228, 370
BsrI ACTGG 2 cut(s) 351, 775
BssECI CCNNGG 4 cut(s) 407, 498, 753, 849
BssMI GATC 6 cut(s) 123, 177, 337, 432, 439, 1060
BssT1I CCWWGG 2 cut(s) 407, 753
Bst2UI CCWGG 3 cut(s) 492, 500, 818
Bst4CI ACNGT 6 cut(s) 375, 578, 622, 715, 907, 998
BstAPI GCANNNNNTGC 1 cut(s) 959
BstAUI TGTACA 2 cut(s) 228, 370
BstC8I GCNNGC 3 cut(s) 84, 115, 355
BstDEI CTNAG 2 cut(s) 915, 963
BstDSI CCRYGG 1 cut(s) 753
BstF5I GGATG 5 cut(s) 47, 388, 403, 547, 1045
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 6 cut(s) 126, 180, 340, 435, 442, 1063
BstMBI GATC 6 cut(s) 123, 177, 337, 432, 439, 1060
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 5 cut(s) 53, 92, 272, 551, 959
BstNI CCWGG 3 cut(s) 492, 500, 818
BstNSI RCATGY 2 cut(s) 235, 394
BstSCI CCNGG 5 cut(s) 134, 490, 498, 816, 848
BstSFI CTRYAG 1 cut(s) 657
BstSLI GKGCMC 1 cut(s) 556
BstV1I GCAGC 8 cut(s) 29, 158, 186, 208, 262, 384, 774, 906
BstV2I GAAGAC 1 cut(s) 242
BstX2I RGATCY 1 cut(s) 177
BstXI CCANNNNNNTGG 1 cut(s) 760
BstYI RGATCY 1 cut(s) 177
BsuI GTATCC 1 cut(s) 336
BsuRI GGCC 2 cut(s) 587, 1006
BtgI CCRYGG 1 cut(s) 753
BtgZI GCGATG 1 cut(s) 94
BtrI CACGTC 2 cut(s) 480, 750
BtsCI GGATG 5 cut(s) 47, 388, 403, 547, 1045
BtsI GCAGTG 2 cut(s) 485, 727
BtsIMutI CAGTG 2 cut(s) 485, 727
BveI ACCTGC 1 cut(s) 264
Cac8I GCNNGC 3 cut(s) 84, 115, 355
CfoI GCGC 1 cut(s) 47
Cfr13I GGNCC 2 cut(s) 585, 1005
Csp6I GTAC 3 cut(s) 229, 371, 574
CspCI CAANNNNNGTGG 2 cut(s) 843, 878
CviAII CATG 5 cut(s) 213, 232, 379, 391, 754
CviQI GTAC 3 cut(s) 229, 371, 574
DdeI CTNAG 2 cut(s) 915, 963
DpnI GATC 6 cut(s) 125, 179, 339, 434, 441, 1062
DpnII GATC 6 cut(s) 123, 177, 337, 432, 439, 1060
DrdI GACNNNNNNGTC 1 cut(s) 713
DseDI GACNNNNNNGTC 1 cut(s) 713
Eco130I CCWWGG 2 cut(s) 407, 753
Eco32I GATATC 1 cut(s) 1012
Eco88I CYCGRG 1 cut(s) 69
EcoRI GAATTC 1 cut(s) 823
EcoRII CCWGG 3 cut(s) 490, 498, 816
EcoRV GATATC 1 cut(s) 1012
EcoT14I CCWWGG 2 cut(s) 407, 753
ErhI CCWWGG 2 cut(s) 407, 753
FaeI CATG 5 cut(s) 216, 235, 382, 394, 757
FatI CATG 5 cut(s) 212, 231, 378, 390, 753
FauI CCCGC 1 cut(s) 709
Fnu4HI GCNGC 9 cut(s) 18, 147, 197, 200, 276, 398, 788, 920, 923
FokI GGATG 5 cut(s) 54, 390, 395, 554, 1052
Fsp4HI GCNGC 9 cut(s) 18, 147, 197, 200, 276, 398, 788, 920, 923
FspBI CTAG 2 cut(s) 83, 114
GlaI GCGC 1 cut(s) 46
GluI GCNGC 9 cut(s) 18, 147, 197, 200, 276, 398, 788, 920, 923
HaeIII GGCC 2 cut(s) 587, 1006
HapII CCGG 4 cut(s) 136, 165, 849, 992
HhaI GCGC 1 cut(s) 47
Hin1II CATG 5 cut(s) 216, 235, 382, 394, 757
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HincII GTYRAC 1 cut(s) 483
HindII GTYRAC 1 cut(s) 483
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 3 cut(s) 159, 359, 663
HpaII CCGG 4 cut(s) 136, 165, 849, 992
HphI GGTGA 2 cut(s) 245, 637
Hpy166II GTNNAC 5 cut(s) 229, 371, 483, 554, 1073
Hpy188I TCNGA 4 cut(s) 282, 342, 524, 916
Hpy188III TCNNGA 5 cut(s) 165, 175, 320, 944, 992
Hpy8I GTNNAC 5 cut(s) 229, 371, 483, 554, 1073
Hpy99I CGWCG 2 cut(s) 209, 719
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 6 cut(s) 375, 578, 622, 715, 907, 998
HpyCH4IV ACGT 3 cut(s) 25, 479, 749
HpyCH4V TGCA 9 cut(s) 259, 400, 416, 554, 659, 683, 790, 795, 953
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 92, 272, 551, 959
HpyF3I CTNAG 2 cut(s) 915, 963
HpySE526I ACGT 3 cut(s) 25, 479, 749
Hsp92II CATG 5 cut(s) 216, 235, 382, 394, 757
HspAI GCGC 1 cut(s) 45
Kpn2I TCCGGA 2 cut(s) 164, 991
Kzo9I GATC 6 cut(s) 123, 177, 337, 432, 439, 1060
LmnI GCTCC 2 cut(s) 167, 387
Lsp1109I GCAGC 8 cut(s) 29, 158, 186, 208, 262, 384, 774, 906
LweI GCATC 6 cut(s) 32, 211, 409, 532, 692, 1030
MaeI CTAG 2 cut(s) 83, 114
MaeII ACGT 3 cut(s) 25, 479, 749
MaeIII GTNAC 2 cut(s) 901, 907
MalI GATC 6 cut(s) 125, 179, 339, 434, 441, 1062
MboI GATC 6 cut(s) 123, 177, 337, 432, 439, 1060
MboII GAAGA 5 cut(s) 242, 295, 298, 908, 1060
MflI RGATCY 1 cut(s) 177
MhlI GDGCHC 1 cut(s) 556
MluCI AATT 7 cut(s) 140, 419, 611, 723, 823, 977, 1082
MlyI GAGTC 2 cut(s) 153, 672
MnlI CCTC 3 cut(s) 110, 749, 922
MroI TCCGGA 2 cut(s) 164, 991
MseI TTAA 1 cut(s) 1080
MslI CAYNNNNRTG 3 cut(s) 524, 752, 795
MspA1I CMGCKG 1 cut(s) 199
MspI CCGG 4 cut(s) 136, 165, 849, 992
MspR9I CCNGG 5 cut(s) 136, 492, 500, 818, 850
Mva1269I GAATGC 2 cut(s) 634, 964
MvaI CCWGG 3 cut(s) 492, 500, 818
MwoI GCNNNNNNNGC 5 cut(s) 53, 92, 272, 551, 959
NciI CCSGG 2 cut(s) 136, 850
NcoI CCATGG 1 cut(s) 753
NdeII GATC 6 cut(s) 123, 177, 337, 432, 439, 1060
NheI GCTAGC 2 cut(s) 82, 113
NlaIII CATG 5 cut(s) 216, 235, 382, 394, 757
NlaIV GGNNCC 4 cut(s) 169, 496, 586, 699
NmuCI GTSAC 1 cut(s) 907
NspI RCATGY 2 cut(s) 235, 394
PaeR7I CTCGAG 1 cut(s) 69
PaqCI CACCTGC 1 cut(s) 264
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PctI GAATGC 2 cut(s) 634, 964
PfeI GAWTC 1 cut(s) 359
PflMI CCANNNNNTGG 1 cut(s) 594
PkrI GCNGC 9 cut(s) 19, 148, 198, 201, 277, 399, 789, 921, 924
Ple19I CGATCG 1 cut(s) 126
PleI GAGTC 2 cut(s) 153, 671
PpsI GAGTC 2 cut(s) 153, 671
Psp6I CCWGG 3 cut(s) 490, 498, 816
PspGI CCWGG 3 cut(s) 490, 498, 816
PspN4I GGNNCC 4 cut(s) 169, 496, 586, 699
PspPI GGNCC 2 cut(s) 585, 1005
PspXI VCTCGAGB 1 cut(s) 69
PstI CTGCAG 1 cut(s) 661
PsuI RGATCY 1 cut(s) 177
PvuI CGATCG 1 cut(s) 126
PvuII CAGCTG 1 cut(s) 199
RsaI GTAC 3 cut(s) 230, 372, 575
RsaNI GTAC 3 cut(s) 229, 371, 574
RseI CAYNNNNRTG 3 cut(s) 524, 752, 795
SaqAI TTAA 1 cut(s) 1080
SatI GCNGC 9 cut(s) 18, 147, 197, 200, 276, 398, 788, 920, 923
Sau3AI GATC 6 cut(s) 123, 177, 337, 432, 439, 1060
Sau96I GGNCC 2 cut(s) 585, 1005
SchI GAGTC 2 cut(s) 153, 672
ScrFI CCNGG 5 cut(s) 136, 492, 500, 818, 850
SduI GDGCHC 1 cut(s) 556
SfaNI GCATC 6 cut(s) 32, 211, 409, 532, 692, 1030
SfcI CTRYAG 1 cut(s) 657
Sfr274I CTCGAG 1 cut(s) 69
SlaI CTCGAG 1 cut(s) 69
SmiMI CAYNNNNRTG 3 cut(s) 524, 752, 795
SmlI CTYRAG 1 cut(s) 69
SmoI CTYRAG 1 cut(s) 69
Sse9I AATT 7 cut(s) 140, 419, 611, 723, 823, 977, 1082
SsiI CCGC 4 cut(s) 224, 266, 702, 923
SspMI CTAG 2 cut(s) 83, 114
StyD4I CCNGG 5 cut(s) 134, 490, 498, 816, 848
StyI CCWWGG 2 cut(s) 407, 753
TaaI ACNGT 6 cut(s) 375, 578, 622, 715, 907, 998
TaiI ACGT 3 cut(s) 28, 482, 752
TaqI TCGA 4 cut(s) 70, 122, 126, 204
TasI AATT 7 cut(s) 140, 419, 611, 723, 823, 977, 1082
TatI WGTACW 2 cut(s) 228, 370
TauI GCSGC 1 cut(s) 925
TfiI GAWTC 1 cut(s) 359
Tru1I TTAA 1 cut(s) 1080
Tru9I TTAA 1 cut(s) 1080
TscAI CASTG 2 cut(s) 492, 734
TseFI GTSAC 1 cut(s) 907
TseI GCWGC 8 cut(s) 17, 146, 196, 199, 275, 397, 787, 919
Tsp45I GTSAC 1 cut(s) 907
TspDTI ATGAA 3 cut(s) 367, 680, 822
TspGWI ACGGA 2 cut(s) 198, 461
TspRI CASTG 2 cut(s) 492, 734
Van91I CCANNNNNTGG 1 cut(s) 594
VneI GTGCAC 1 cut(s) 552
XapI RAATTY 3 cut(s) 140, 823, 977
XceI RCATGY 2 cut(s) 235, 394
XhoI CTCGAG 1 cut(s) 69
XspI CTAG 2 cut(s) 83, 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.