Rh6BG192600

Oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0003907
Physical Location & Seq
Forward (+)
36156338 .. 36158581
2244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG192600.1

Sequence Viewer

Length: 1083 bp
ATGTCAGCGGAATCACCAATCCGGTTCGGAATTCTGGGCTGCGCCGAAATAGCCCGAAAGGTATCACGTGCCATCACTCTGGCCCCCAACGCCACTCTTTACGCCGTCGGAAGCCGTTCCATTGATAAGGCCACCGCGTTCGCCAGGGCCAACAACTTCCCTCCCAACGCCAGAATCTACGGCTCCTACGACGCCCTTTTGGACGACCCTGACGTCGACGCCGTCTATGTCCCCCTCCCTACCAGCCTGCACATCCAGTGGGCCGTCGTCGCGGCCCAGAAGAAGAAGCACATCTTGCTTGAGAAGCCCGTCGCGCTGAACGTGGCGGAGTTTGACAGAATCGTTGAGGCCTGTGAATCCAATGGGGTGCAGCTCATGGATGGTACCATGTGGATGCATCATCCCAGGACTGCCAAGATGTGTGAGTTTCTCTCTGATGCCAACCGTTTTGGTCAGCTCAAAACGATACACACCTGCTTTACATTTGCTGCTGATCCTGATTTCCTGAAGAATGATATTCGCGTGAAGCCAGACCTTGATGCTCATGGTGCTCTTGGGGATGCAGGGTGGTACTGCATCAGGGCAATCCTGTGGGCTAATAACTATGAACTGCCAAAGACAGTAGTTGCCTTGCGTGGCCCTATATTGAATGAAGCTGGGGTGATTTTATCTTGTGGAGCTTCTCTACACTGGGAAGATGGGAAAGTGGGAACCTTCCACTGCTCTTTCTTGTCAAGTTTGACAATGGATATAACTGCTATTGGTACAAAGGGAACGTTACATGCCAGTGGTTTTATTATCCCTCATCGAGAGAAAGAGGCTTCTTTCTCTGTAGCTTCTGAAACTGGATTTGATGAATTTGTGACCAGTTGGGTACCACCGCCAAGTGATCACATTGTTACCACAGATCTTCCACAGGAGGCTCTTATGGTAACAGAGTTTTCTCGCTTGGTAGGGATTGTTAAAAGTGGTTCTACACCTGAGAAGAAATGGCCAATCCTTAGCAGGAAGACACAGCTAGTACTTGATGCTGTCAAGGCATCAATTAACAAGGGTTTTGACCCTATACCTATTGAATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GFO_IDH_MocA

Protein Analysis

360

Amino Acids

39.39

Weight (kDa)

6.13

Isoelectric Point (pI)

33.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GFO_IDH_MocA PF01408 7 - 126 3.1e-22 Oxidoreductase family, NAD-binding Rossmann fold
GFO_IDH_MocA_C3 PF22725 146 - 260 2.6e-12 GFO/IDH/MocA C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 482
AasI GACNNNNNNGTC 1 cut(s) 212
AatII GACGTC 1 cut(s) 216
Acc36I ACCTGC 1 cut(s) 482
Acc65I GGTACC 2 cut(s) 383, 874
AccB1I GGYRCC 2 cut(s) 383, 874
AccI GTMKAC 1 cut(s) 216
AccII CGCG 4 cut(s) 137, 272, 314, 522
AciI CCGC 5 cut(s) 8, 135, 272, 326, 881
AclI AACGTT 1 cut(s) 776
AclWI GGATC 1 cut(s) 488
AcoI YGGCCR 1 cut(s) 992
AcsI RAATTY 2 cut(s) 30, 857
AcuI CTGAAG 1 cut(s) 527
AcvI CACGTG 1 cut(s) 68
AcyI GRCGYC 3 cut(s) 192, 213, 219
AfaI GTAC 5 cut(s) 385, 572, 766, 876, 1023
AgsI TTSAA 2 cut(s) 649, 1076
AjnI CCWGG 2 cut(s) 143, 404
AluBI AGCT 6 cut(s) 373, 457, 656, 680, 836, 1018
AluI AGCT 6 cut(s) 373, 457, 656, 680, 836, 1018
Alw21I GWGCWC 1 cut(s) 553
AlwI GGATC 1 cut(s) 488
AoxI GGCC 8 cut(s) 81, 129, 147, 261, 273, 348, 637, 992
ApeKI GCWGC 3 cut(s) 39, 370, 488
ApoI RAATTY 2 cut(s) 30, 857
Asp700I GAANNNNTTC 1 cut(s) 115
Asp718I GGTACC 2 cut(s) 383, 874
AspLEI GCGC 2 cut(s) 44, 316
AspS9I GGNCC 5 cut(s) 82, 147, 261, 274, 638
AsuHPI GGTGA 2 cut(s) 6, 673
BalI TGGCCA 1 cut(s) 994
BanI GGYRCC 2 cut(s) 383, 874
BbrPI CACGTG 1 cut(s) 68
BbsI GAAGAC 1 cut(s) 1016
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 3 cut(s) 26, 382, 475
BccI CCATC 3 cut(s) 80, 374, 692
BceAI ACGGC 5 cut(s) 89, 99, 196, 206, 248
BciT130I CCWGG 2 cut(s) 145, 406
BclI TGATCA 1 cut(s) 889
BfaI CTAG 1 cut(s) 1019
BfmI CTRYAG 1 cut(s) 831
BfuAI ACCTGC 1 cut(s) 482
BglII AGATCT 1 cut(s) 907
BisI GCNGC 4 cut(s) 40, 273, 371, 489
BlsI GCNGC 4 cut(s) 41, 274, 372, 490
BmcAI AGTACT 1 cut(s) 1023
Bme1390I CCNGG 2 cut(s) 145, 406
BmgT120I GGNCC 5 cut(s) 82, 147, 261, 274, 638
BmiI GGNNCC 5 cut(s) 84, 184, 385, 712, 876
BmrFI CCNGG 2 cut(s) 145, 406
BmrI ACTGGG 1 cut(s) 700
BmsI GCATC 8 cut(s) 384, 406, 427, 529, 550, 585, 1018, 1049
BmuI ACTGGG 1 cut(s) 700
BpiI GAAGAC 1 cut(s) 1016
BplI GAGNNNNNCTC 2 cut(s) 416, 448
Bpu10I CCTNAGC 1 cut(s) 1001
BpuEI CTTGAG 1 cut(s) 320
BsaAI YACGTR 1 cut(s) 68
BsaHI GRCGYC 3 cut(s) 192, 213, 219
BsaJI CCNNGG 2 cut(s) 144, 404
BsaWI WCCGGW 1 cut(s) 21
Bse1I ACTGG 5 cut(s) 256, 695, 786, 850, 867
BseBI CCWGG 2 cut(s) 145, 406
BseDI CCNNGG 2 cut(s) 144, 404
BseGI GGATG 5 cut(s) 252, 385, 399, 400, 565
BseMII CTCAG 1 cut(s) 972
BseNI ACTGG 5 cut(s) 256, 695, 786, 850, 867
BseXI GCAGC 3 cut(s) 26, 382, 475
BseYI CCCAGC 1 cut(s) 656
BsgI GTGCAG 2 cut(s) 233, 389
Bsh1236I CGCG 4 cut(s) 137, 272, 314, 522
BshFI GGCC 8 cut(s) 83, 131, 149, 263, 275, 350, 639, 994
BshNI GGYRCC 2 cut(s) 383, 874
BsiHKAI GWGCWC 1 cut(s) 553
BsiSI CCGG 1 cut(s) 22
BslFI GGGAC 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 215
BsnI GGCC 8 cut(s) 83, 131, 149, 263, 275, 350, 639, 994
Bsp1286I GDGCHC 1 cut(s) 553
Bsp143I GATC 3 cut(s) 493, 889, 907
BspACI CCGC 5 cut(s) 8, 135, 272, 326, 881
BspANI GGCC 8 cut(s) 83, 131, 149, 263, 275, 350, 639, 994
BspCNI CTCAG 1 cut(s) 973
BspFNI CGCG 4 cut(s) 137, 272, 314, 522
BspLI GGNNCC 5 cut(s) 84, 184, 385, 712, 876
BspMI ACCTGC 1 cut(s) 482
BspPI GGATC 1 cut(s) 488
BspT107I GGYRCC 2 cut(s) 383, 874
BsrI ACTGG 5 cut(s) 256, 695, 786, 850, 867
BssECI CCNNGG 2 cut(s) 144, 404
BssMI GATC 3 cut(s) 493, 889, 907
BssNI GRCGYC 3 cut(s) 192, 213, 219
Bst2UI CCWGG 2 cut(s) 145, 406
Bst4CI ACNGT 2 cut(s) 446, 622
BstACI GRCGYC 3 cut(s) 192, 213, 219
BstAPI GCANNNNNTGC 1 cut(s) 295
BstBAI YACGTR 1 cut(s) 68
BstC8I GCNNGC 1 cut(s) 248
BstDEI CTNAG 2 cut(s) 981, 1001
BstF5I GGATG 5 cut(s) 252, 385, 399, 400, 565
BstFNI CGCG 4 cut(s) 137, 272, 314, 522
BstHHI GCGC 2 cut(s) 44, 316
BstKTI GATC 3 cut(s) 496, 892, 910
BstMBI GATC 3 cut(s) 493, 889, 907
BstMWI GCNNNNNNNGC 8 cut(s) 50, 89, 269, 295, 304, 313, 548, 1037
BstNI CCWGG 2 cut(s) 145, 406
BstNSI RCATGY 1 cut(s) 785
BstSCI CCNGG 2 cut(s) 143, 404
BstSFI CTRYAG 1 cut(s) 831
BstUI CGCG 4 cut(s) 137, 272, 314, 522
BstV1I GCAGC 3 cut(s) 26, 382, 475
BstV2I GAAGAC 1 cut(s) 1016
BstX2I RGATCY 1 cut(s) 907
BstXI CCANNNNNNTGG 1 cut(s) 79
BstYI RGATCY 1 cut(s) 907
BsuRI GGCC 8 cut(s) 83, 131, 149, 263, 275, 350, 639, 994
BtsCI GGATG 5 cut(s) 252, 385, 399, 400, 565
BtsI GCAGTG 1 cut(s) 718
BtsIMutI CAGTG 4 cut(s) 263, 688, 718, 793
BveI ACCTGC 1 cut(s) 482
Cac8I GCNNGC 1 cut(s) 248
CfoI GCGC 2 cut(s) 44, 316
Cfr13I GGNCC 5 cut(s) 82, 147, 261, 274, 638
CseI GACGC 2 cut(s) 200, 227
Csp6I GTAC 5 cut(s) 384, 571, 765, 875, 1022
CviAII CATG 4 cut(s) 376, 388, 545, 782
CviQI GTAC 5 cut(s) 384, 571, 765, 875, 1022
DdeI CTNAG 2 cut(s) 981, 1001
DpnI GATC 3 cut(s) 495, 891, 909
DpnII GATC 3 cut(s) 493, 889, 907
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
EaeI YGGCCR 1 cut(s) 992
EciI GGCGGA 1 cut(s) 341
Eco147I AGGCCT 1 cut(s) 350
Eco57I CTGAAG 1 cut(s) 527
Eco72I CACGTG 1 cut(s) 68
EcoRI GAATTC 1 cut(s) 30
EcoRII CCWGG 2 cut(s) 143, 404
EcoT22I ATGCAT 1 cut(s) 399
FaeI CATG 4 cut(s) 379, 391, 548, 785
FalI AAGNNNNNCTT 2 cut(s) 278, 310
FaqI GGGAC 1 cut(s) 215
FatI CATG 4 cut(s) 375, 387, 544, 781
FbaI TGATCA 1 cut(s) 889
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 4 cut(s) 40, 273, 371, 489
FokI GGATG 5 cut(s) 239, 387, 392, 406, 572
Fsp4HI GCNGC 4 cut(s) 40, 273, 371, 489
FspBI CTAG 1 cut(s) 1019
GlaI GCGC 2 cut(s) 43, 315
GluI GCNGC 4 cut(s) 40, 273, 371, 489
GsaI CCCAGC 1 cut(s) 660
HaeIII GGCC 8 cut(s) 83, 131, 149, 263, 275, 350, 639, 994
HapII CCGG 1 cut(s) 22
HgaI GACGC 2 cut(s) 200, 227
HhaI GCGC 2 cut(s) 44, 316
Hin1I GRCGYC 3 cut(s) 192, 213, 219
Hin1II CATG 4 cut(s) 379, 391, 548, 785
Hin6I GCGC 2 cut(s) 42, 314
HinP1I GCGC 2 cut(s) 42, 314
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 4 cut(s) 11, 174, 339, 356
HpaII CCGG 1 cut(s) 22
HphI GGTGA 2 cut(s) 6, 673
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 4 cut(s) 29, 110, 436, 841
Hpy188III TCNNGA 3 cut(s) 497, 505, 809
Hpy8I GTNNAC 1 cut(s) 217
Hpy99I CGWCG 7 cut(s) 110, 194, 218, 221, 269, 272, 314
HpyAV CCTTC 1 cut(s) 724
HpyCH4III ACNGT 2 cut(s) 446, 622
HpyCH4IV ACGT 4 cut(s) 67, 213, 321, 776
HpyCH4V TGCA 5 cut(s) 250, 370, 397, 563, 576
HpyF10VI GCNNNNNNNGC 8 cut(s) 50, 89, 269, 295, 304, 313, 548, 1037
HpyF3I CTNAG 2 cut(s) 981, 1001
HpySE526I ACGT 4 cut(s) 67, 213, 321, 776
Hsp92I GRCGYC 3 cut(s) 192, 213, 219
Hsp92II CATG 4 cut(s) 379, 391, 548, 785
HspAI GCGC 2 cut(s) 42, 314
KpnI GGTACC 2 cut(s) 387, 878
Ksp22I TGATCA 1 cut(s) 889
Kzo9I GATC 3 cut(s) 493, 889, 907
LmnI GCTCC 2 cut(s) 188, 677
Lsp1109I GCAGC 3 cut(s) 26, 382, 475
LweI GCATC 8 cut(s) 384, 406, 427, 529, 550, 585, 1018, 1049
MaeI CTAG 1 cut(s) 1019
MaeII ACGT 4 cut(s) 67, 213, 321, 776
MaeIII GTNAC 4 cut(s) 777, 862, 898, 931
MalI GATC 3 cut(s) 495, 891, 909
MboI GATC 3 cut(s) 493, 889, 907
MboII GAAGA 7 cut(s) 292, 295, 520, 707, 902, 997, 1021
MflI RGATCY 1 cut(s) 907
MhlI GDGCHC 1 cut(s) 553
MlsI TGGCCA 1 cut(s) 994
MluCI AATT 3 cut(s) 30, 857, 1044
MluNI TGGCCA 1 cut(s) 994
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 6 cut(s) 171, 245, 340, 811, 813, 913
Mox20I TGGCCA 1 cut(s) 994
Mph1103I ATGCAT 1 cut(s) 399
MroXI GAANNNNTTC 1 cut(s) 115
MscI TGGCCA 1 cut(s) 994
MseI TTAA 3 cut(s) 963, 1047, 1081
MslI CAYNNNNRTG 2 cut(s) 392, 786
Msp20I TGGCCA 1 cut(s) 994
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 1 cut(s) 22
MspR9I CCNGG 2 cut(s) 145, 406
MvaI CCWGG 2 cut(s) 145, 406
MvnI CGCG 4 cut(s) 137, 272, 314, 522
MwoI GCNNNNNNNGC 8 cut(s) 50, 89, 269, 295, 304, 313, 548, 1037
NdeII GATC 3 cut(s) 493, 889, 907
NlaIII CATG 4 cut(s) 379, 391, 548, 785
NlaIV GGNNCC 5 cut(s) 84, 184, 385, 712, 876
NmuCI GTSAC 1 cut(s) 862
NsiI ATGCAT 1 cut(s) 399
NspI RCATGY 1 cut(s) 785
PaqCI CACCTGC 1 cut(s) 482
PceI AGGCCT 1 cut(s) 350
PcsI WCGNNNNNNNCGW 4 cut(s) 186, 210, 219, 318
PdmI GAANNNNTTC 1 cut(s) 115
PfeI GAWTC 4 cut(s) 11, 174, 339, 356
PflFI GACNNNGTC 1 cut(s) 221
PkrI GCNGC 4 cut(s) 41, 274, 372, 490
PmaCI CACGTG 1 cut(s) 68
PmlI CACGTG 1 cut(s) 68
Ppu21I YACGTR 1 cut(s) 68
Psp1406I AACGTT 1 cut(s) 776
Psp6I CCWGG 2 cut(s) 143, 404
PspCI CACGTG 1 cut(s) 68
PspFI CCCAGC 1 cut(s) 656
PspGI CCWGG 2 cut(s) 143, 404
PspN4I GGNNCC 5 cut(s) 84, 184, 385, 712, 876
PspPI GGNCC 5 cut(s) 82, 147, 261, 274, 638
PsuI RGATCY 1 cut(s) 907
PsyI GACNNNGTC 1 cut(s) 221
RsaI GTAC 5 cut(s) 385, 572, 766, 876, 1023
RsaNI GTAC 5 cut(s) 384, 571, 765, 875, 1022
RseI CAYNNNNRTG 2 cut(s) 392, 786
SalI GTCGAC 1 cut(s) 215
SaqAI TTAA 3 cut(s) 963, 1047, 1081
SatI GCNGC 4 cut(s) 40, 273, 371, 489
Sau3AI GATC 3 cut(s) 493, 889, 907
Sau96I GGNCC 5 cut(s) 82, 147, 261, 274, 638
ScaI AGTACT 1 cut(s) 1023
ScrFI CCNGG 2 cut(s) 145, 406
SduI GDGCHC 1 cut(s) 553
SfaNI GCATC 8 cut(s) 384, 406, 427, 529, 550, 585, 1018, 1049
SfcI CTRYAG 1 cut(s) 831
SgrDI CGTCGACG 1 cut(s) 215
SmiMI CAYNNNNRTG 2 cut(s) 392, 786
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
Sse9I AATT 3 cut(s) 30, 857, 1044
SseBI AGGCCT 1 cut(s) 350
SsiI CCGC 5 cut(s) 8, 135, 272, 326, 881
SspI AATATT 1 cut(s) 1079
SspMI CTAG 1 cut(s) 1019
StuI AGGCCT 1 cut(s) 350
StyD4I CCNGG 2 cut(s) 143, 404
TaaI ACNGT 2 cut(s) 446, 622
TaiI ACGT 4 cut(s) 70, 216, 324, 779
TaqI TCGA 2 cut(s) 216, 808
TasI AATT 3 cut(s) 30, 857, 1044
TatI WGTACW 1 cut(s) 1021
TauI GCSGC 1 cut(s) 275
TfiI GAWTC 4 cut(s) 11, 174, 339, 356
Tru1I TTAA 3 cut(s) 963, 1047, 1081
Tru9I TTAA 3 cut(s) 963, 1047, 1081
TscAI CASTG 4 cut(s) 263, 695, 725, 793
TseFI GTSAC 1 cut(s) 862
TseI GCWGC 3 cut(s) 39, 370, 488
Tsp45I GTSAC 1 cut(s) 862
TspDTI ATGAA 3 cut(s) 621, 666, 870
TspRI CASTG 4 cut(s) 263, 695, 725, 793
Tth111I GACNNNGTC 1 cut(s) 221
XapI RAATTY 2 cut(s) 30, 857
XceI RCATGY 1 cut(s) 785
XmiI GTMKAC 1 cut(s) 216
XmnI GAANNNNTTC 1 cut(s) 115
XspI CTAG 1 cut(s) 1019
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 1 cut(s) 1023
Zsp2I ATGCAT 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.