MD10G1042200.v1.1

Oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003907
Physical Location & Seq
Forward (+)
5442722 .. 5446539
3818 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1042200.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGTCCGCCGAAACGCCGATCCGATTCGGAATTGTGGGCTGCGCCGAAATAGCCCGGAAAGTGTCGCGGGCCATAACTCGAGCCCCAAACGCGACACTCTACGCCGTCGGAAGCCGTTCTATCGACAAGGCTACGGCGTTCGCCAAGGCCAACAATTTCCCTCCCACCGCCAAGATCTACGGCTCCTACGACGCCGTTCTGGACGATCCCGACGTTGACGCCGTCTATGTCCCGCTCCCCACCAGCCTCCACATCAAGTGGGCCGTCCTCGCCGCCCAGAAGAAGAAGCACATCCTGCTCGAGAAGCCCGTGGCGCTCAACGTAGCGGAGTTTGACAAGATCGCTGAGGCGTGCGAATCCAATGGGGTGCAGATCATGGACGGTACCATGTGGATGCACCACCCCAGGACTGCCAAGATGTCCGAATTTCTCGCCGATGCTAACCGTTTTGGTCAGCTCAGAACGATACACACTGTCTTTACATTTCCTTGTGATCCTGAATTTTTCAAGAATGATATCCGGGTGAAGCCAGACCTTGATGCTCATGGGGCTCTTGGGGATGCAGGGTGGTACTGTATAAGAGCAATTTTGTGGGCCAATAACTATGAACTGCCAAAAACTGTAATTGCTTTGCGGGACCCTGTGCTTAATGAAGCAGGCGTGATTTTATCTTGTGGGGCGTCTTTGCATTGGGAAGATGGGAAAGTGGCAACCTTCCACTGCTCTTTCTTATCAAGTTTGACGATGGATATAACTGCGGTCGGTACAAAGGGAACTCTGCATGTTGATGGTTTTGTTATCCCTCATGAAGAGAAAGAAGCCGCTTTTTCTGCAGCTTCACAAAGTGGATTTGATGAATTTGTCACCTGTTGGGTTCCATCACCAAGTCGTCACATTGTGACCACAGATCTTCCGCAGGAGGTCCTCATGGTAAGAGAGTTTGCTCGTTTGGTAGGGGCTATTAAAAATGGTGCAAAACCTGAGAAGAAGTGGCCAACCCTTAGTCGGAAGACACAGATCGTACTCGATGCTGTCAAGGCATCAATTGCCAAGGGTTTTGAATCCATTGATATCGCATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GFO_IDH_MocA

Protein Analysis

361

Amino Acids

39.46

Weight (kDa)

6.6

Isoelectric Point (pI)

35.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GFO_IDH_MocA PF01408 7 - 126 1.2e-22 Oxidoreductase family, NAD-binding Rossmann fold
GFO_IDH_MocA_C3 PF22725 145 - 260 7.9e-13 GFO/IDH/MocA C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 383
AccB1I GGYRCC 1 cut(s) 383
AccBSI CCGCTC 1 cut(s) 235
AccII CGCG 2 cut(s) 67, 92
AclWI GGATC 3 cut(s) 13, 200, 488
AcoI YGGCCR 1 cut(s) 992
AcsI RAATTY 3 cut(s) 425, 500, 857
AcyI GRCGYC 3 cut(s) 192, 219, 680
AdeI CACNNNGTG 2 cut(s) 845, 898
AfaI GTAC 4 cut(s) 385, 572, 766, 1023
AfiI CCNNNNNNNGG 1 cut(s) 1005
AgsI TTSAA 2 cut(s) 508, 1061
AjnI CCWGG 1 cut(s) 404
AluBI AGCT 2 cut(s) 457, 836
AluI AGCT 2 cut(s) 457, 836
AlwI GGATC 3 cut(s) 13, 200, 488
Ama87I CYCGRG 2 cut(s) 78, 299
AoxI GGCC 5 cut(s) 69, 147, 261, 594, 992
ApeKI GCWGC 2 cut(s) 39, 833
ApoI RAATTY 3 cut(s) 425, 500, 857
Asp700I GAANNNNTTC 1 cut(s) 115
Asp718I GGTACC 1 cut(s) 383
AspLEI GCGC 2 cut(s) 44, 316
AspS9I GGNCC 5 cut(s) 69, 261, 594, 637, 922
AsuC2I CCSGG 2 cut(s) 55, 521
AsuHPI GGTGA 3 cut(s) 535, 856, 873
AvaI CYCGRG 2 cut(s) 78, 299
AvaII GGWCC 2 cut(s) 637, 922
BalI TGGCCA 1 cut(s) 994
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 2 cut(s) 85, 553
BbsI GAAGAC 1 cut(s) 1016
BbvCI CCTCAGC 1 cut(s) 345
BbvI GCAGC 2 cut(s) 26, 845
BccI CCATC 4 cut(s) 692, 739, 782, 886
BceAI ACGGC 7 cut(s) 89, 99, 150, 179, 196, 206, 248
BciT130I CCWGG 1 cut(s) 406
BcnI CCSGG 2 cut(s) 55, 521
BfmI CTRYAG 1 cut(s) 831
BfoI RGCGCY 1 cut(s) 317
BglII AGATCT 2 cut(s) 174, 907
BisI GCNGC 4 cut(s) 40, 273, 822, 834
BlsI GCNGC 4 cut(s) 41, 274, 823, 835
Bme1390I CCNGG 3 cut(s) 55, 406, 521
Bme18I GGWCC 2 cut(s) 637, 922
BmeT110I CYCGRG 2 cut(s) 78, 299
BmgT120I GGNCC 5 cut(s) 69, 261, 594, 637, 922
BmiI GGNNCC 5 cut(s) 184, 385, 638, 639, 876
BmrFI CCNGG 3 cut(s) 55, 406, 521
BmsI GCATC 6 cut(s) 384, 427, 529, 550, 1018, 1049
BpiI GAAGAC 1 cut(s) 1016
Bpu10I CCTNAGC 1 cut(s) 345
BpuMI CCSGG 2 cut(s) 55, 521
BsaHI GRCGYC 3 cut(s) 192, 219, 680
BsaJI CCNNGG 4 cut(s) 144, 309, 404, 1050
Bsc4I CCNNNNNNNGG 1 cut(s) 1005
BseBI CCWGG 1 cut(s) 406
BseDI CCNNGG 4 cut(s) 144, 309, 404, 1050
BseGI GGATG 3 cut(s) 291, 399, 565
BseLI CCNNNNNNNGG 1 cut(s) 1005
BseMII CTCAG 3 cut(s) 336, 472, 972
BseXI GCAGC 2 cut(s) 26, 845
BsgI GTGCAG 1 cut(s) 389
Bsh1236I CGCG 2 cut(s) 67, 92
Bsh1285I CGRYCG 1 cut(s) 762
BshFI GGCC 5 cut(s) 71, 149, 263, 596, 994
BshNI GGYRCC 1 cut(s) 383
BsiEI CGRYCG 1 cut(s) 762
BsiHKCI CYCGRG 2 cut(s) 78, 299
BsiSI CCGG 2 cut(s) 55, 520
BslFI GGGAC 2 cut(s) 215, 650
BslI CCNNNNNNNGG 1 cut(s) 1005
BsmFI GGGAC 2 cut(s) 215, 650
BsnI GGCC 5 cut(s) 71, 149, 263, 596, 994
BsoBI CYCGRG 2 cut(s) 78, 299
Bsp1286I GDGCHC 2 cut(s) 85, 553
Bsp143I GATC 8 cut(s) 18, 174, 205, 339, 372, 493, 907, 1017
BspANI GGCC 5 cut(s) 71, 149, 263, 596, 994
BspCNI CTCAG 3 cut(s) 337, 471, 973
BspFNI CGCG 2 cut(s) 67, 92
BspHI TCATGA 1 cut(s) 805
BspLI GGNNCC 5 cut(s) 184, 385, 638, 639, 876
BspMAI CTGCAG 1 cut(s) 835
BspPI GGATC 3 cut(s) 13, 200, 488
BspT107I GGYRCC 1 cut(s) 383
BsrBI CCGCTC 1 cut(s) 235
BssECI CCNNGG 4 cut(s) 144, 309, 404, 1050
BssMI GATC 8 cut(s) 18, 174, 205, 339, 372, 493, 907, 1017
BssNI GRCGYC 3 cut(s) 192, 219, 680
BssT1I CCWWGG 2 cut(s) 144, 1050
Bst2UI CCWGG 1 cut(s) 406
Bst4CI ACNGT 5 cut(s) 383, 446, 475, 575, 622
Bst6I CTCTTC 1 cut(s) 804
BstACI GRCGYC 3 cut(s) 192, 219, 680
BstAPI GCANNNNNTGC 2 cut(s) 295, 1046
BstC8I GCNNGC 3 cut(s) 69, 352, 658
BstDEI CTNAG 4 cut(s) 345, 458, 981, 1001
BstDSI CCRYGG 1 cut(s) 309
BstF5I GGATG 3 cut(s) 291, 399, 565
BstFNI CGCG 2 cut(s) 67, 92
BstH2I RGCGCY 1 cut(s) 317
BstHHI GCGC 2 cut(s) 44, 316
BstKTI GATC 8 cut(s) 21, 177, 208, 342, 375, 496, 910, 1020
BstMBI GATC 8 cut(s) 18, 174, 205, 339, 372, 493, 907, 1017
BstMCI CGRYCG 1 cut(s) 762
BstNI CCWGG 1 cut(s) 406
BstNSI RCATGY 1 cut(s) 785
BstSCI CCNGG 3 cut(s) 53, 404, 519
BstSFI CTRYAG 1 cut(s) 831
BstUI CGCG 2 cut(s) 67, 92
BstV1I GCAGC 2 cut(s) 26, 845
BstV2I GAAGAC 1 cut(s) 1016
BstX2I RGATCY 2 cut(s) 174, 907
BstYI RGATCY 2 cut(s) 174, 907
BsuRI GGCC 5 cut(s) 71, 149, 263, 596, 994
BtgI CCRYGG 1 cut(s) 309
BtsCI GGATG 3 cut(s) 291, 399, 565
BtsI GCAGTG 1 cut(s) 718
BtsIMutI CAGTG 2 cut(s) 471, 718
Cac8I GCNNGC 3 cut(s) 69, 352, 658
CciI TCATGA 1 cut(s) 805
CfoI GCGC 2 cut(s) 44, 316
Cfr13I GGNCC 5 cut(s) 69, 261, 594, 637, 922
CseI GACGC 3 cut(s) 200, 227, 669
Csp6I GTAC 4 cut(s) 384, 571, 765, 1022
CviAII CATG 6 cut(s) 376, 388, 545, 782, 806, 928
CviQI GTAC 4 cut(s) 384, 571, 765, 1022
DdeI CTNAG 4 cut(s) 345, 458, 981, 1001
DpnI GATC 8 cut(s) 20, 176, 207, 341, 374, 495, 909, 1019
DpnII GATC 8 cut(s) 18, 174, 205, 339, 372, 493, 907, 1017
DraIII CACNNNGTG 2 cut(s) 845, 898
EaeI YGGCCR 1 cut(s) 992
Eam1104I CTCTTC 1 cut(s) 804
EarI CTCTTC 1 cut(s) 804
Eco130I CCWWGG 2 cut(s) 144, 1050
Eco24I GRGCYC 2 cut(s) 85, 553
Eco32I GATATC 2 cut(s) 517, 1072
Eco47I GGWCC 2 cut(s) 637, 922
Eco88I CYCGRG 2 cut(s) 78, 299
EcoO109I RGGNCCY 2 cut(s) 637, 922
EcoRII CCWGG 1 cut(s) 404
EcoRV GATATC 2 cut(s) 517, 1072
EcoT14I CCWWGG 2 cut(s) 144, 1050
EcoT38I GRGCYC 2 cut(s) 85, 553
ErhI CCWWGG 2 cut(s) 144, 1050
FaeI CATG 6 cut(s) 379, 391, 548, 785, 809, 931
FaqI GGGAC 2 cut(s) 215, 650
FatI CATG 6 cut(s) 375, 387, 544, 781, 805, 927
FauI CCCGC 3 cut(s) 60, 240, 627
Fnu4HI GCNGC 4 cut(s) 40, 273, 822, 834
FokI GGATG 3 cut(s) 278, 406, 572
FriOI GRGCYC 2 cut(s) 85, 553
Fsp4HI GCNGC 4 cut(s) 40, 273, 822, 834
GlaI GCGC 2 cut(s) 43, 315
GluI GCNGC 4 cut(s) 40, 273, 822, 834
HaeII RGCGCY 1 cut(s) 317
HaeIII GGCC 5 cut(s) 71, 149, 263, 596, 994
HapII CCGG 2 cut(s) 55, 520
HgaI GACGC 3 cut(s) 200, 227, 669
HhaI GCGC 2 cut(s) 44, 316
Hin1I GRCGYC 3 cut(s) 192, 219, 680
Hin1II CATG 6 cut(s) 379, 391, 548, 785, 809, 931
Hin6I GCGC 2 cut(s) 42, 314
HinP1I GCGC 2 cut(s) 42, 314
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 3 cut(s) 24, 356, 1061
HpaII CCGG 2 cut(s) 55, 520
HphI GGTGA 3 cut(s) 535, 856, 873
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 6 cut(s) 23, 29, 110, 424, 461, 1008
Hpy188III TCNNGA 6 cut(s) 200, 209, 301, 497, 508, 806
Hpy8I GTNNAC 1 cut(s) 217
Hpy99I CGWCG 3 cut(s) 110, 194, 215
HpyAV CCTTC 1 cut(s) 724
HpyCH4III ACNGT 5 cut(s) 383, 446, 475, 575, 622
HpyCH4IV ACGT 2 cut(s) 213, 321
HpyCH4V TGCA 7 cut(s) 370, 397, 563, 688, 781, 833, 974
HpyF3I CTNAG 4 cut(s) 345, 458, 981, 1001
HpySE526I ACGT 2 cut(s) 213, 321
Hsp92I GRCGYC 3 cut(s) 192, 219, 680
Hsp92II CATG 6 cut(s) 379, 391, 548, 785, 809, 931
HspAI GCGC 2 cut(s) 42, 314
KflI GGGWCCC 1 cut(s) 637
KpnI GGTACC 1 cut(s) 387
Kzo9I GATC 8 cut(s) 18, 174, 205, 339, 372, 493, 907, 1017
LmnI GCTCC 2 cut(s) 188, 240
Lsp1109I GCAGC 2 cut(s) 26, 845
LweI GCATC 6 cut(s) 384, 427, 529, 550, 1018, 1049
MaeII ACGT 2 cut(s) 213, 321
MaeIII GTNAC 3 cut(s) 862, 890, 898
MalI GATC 8 cut(s) 20, 176, 207, 341, 374, 495, 909, 1019
MbiI CCGCTC 1 cut(s) 235
MboI GATC 8 cut(s) 18, 174, 205, 339, 372, 493, 907, 1017
MboII GAAGA 7 cut(s) 292, 295, 707, 821, 902, 997, 1021
MfeI CAATTG 1 cut(s) 1044
MflI RGATCY 2 cut(s) 174, 907
MhlI GDGCHC 2 cut(s) 85, 553
MlsI TGGCCA 1 cut(s) 994
MluCI AATT 8 cut(s) 30, 154, 425, 500, 585, 624, 857, 1044
MluNI TGGCCA 1 cut(s) 994
MmeI TCCRAC 2 cut(s) 88, 986
MnlI CCTC 7 cut(s) 171, 257, 278, 340, 813, 913, 935
Mox20I TGGCCA 1 cut(s) 994
MroXI GAANNNNTTC 1 cut(s) 115
MscI TGGCCA 1 cut(s) 994
MseI TTAA 3 cut(s) 648, 963, 1081
MslI CAYNNNNRTG 2 cut(s) 392, 786
Msp20I TGGCCA 1 cut(s) 994
MspI CCGG 2 cut(s) 55, 520
MspR9I CCNGG 3 cut(s) 55, 406, 521
MunI CAATTG 1 cut(s) 1044
MvaI CCWGG 1 cut(s) 406
MvnI CGCG 2 cut(s) 67, 92
NciI CCSGG 2 cut(s) 55, 521
NdeII GATC 8 cut(s) 18, 174, 205, 339, 372, 493, 907, 1017
NlaIII CATG 6 cut(s) 379, 391, 548, 785, 809, 931
NlaIV GGNNCC 5 cut(s) 184, 385, 638, 639, 876
NmuCI GTSAC 3 cut(s) 862, 890, 898
NspI RCATGY 1 cut(s) 785
PaeR7I CTCGAG 2 cut(s) 78, 299
PagI TCATGA 1 cut(s) 805
PcsI WCGNNNNNNNCGW 4 cut(s) 186, 210, 219, 306
PdmI GAANNNNTTC 1 cut(s) 115
PfeI GAWTC 3 cut(s) 24, 356, 1061
PflFI GACNNNGTC 1 cut(s) 221
PkrI GCNGC 4 cut(s) 41, 274, 823, 835
PpuMI RGGWCCY 2 cut(s) 637, 922
Psp5II RGGWCCY 2 cut(s) 637, 922
Psp6I CCWGG 1 cut(s) 404
PspGI CCWGG 1 cut(s) 404
PspN4I GGNNCC 5 cut(s) 184, 385, 638, 639, 876
PspPI GGNCC 5 cut(s) 69, 261, 594, 637, 922
PspPPI RGGWCCY 2 cut(s) 637, 922
PspXI VCTCGAGB 1 cut(s) 78
PstI CTGCAG 1 cut(s) 835
PsuI RGATCY 2 cut(s) 174, 907
PsyI GACNNNGTC 1 cut(s) 221
RsaI GTAC 4 cut(s) 385, 572, 766, 1023
RsaNI GTAC 4 cut(s) 384, 571, 765, 1022
RseI CAYNNNNRTG 2 cut(s) 392, 786
SaqAI TTAA 3 cut(s) 648, 963, 1081
SatI GCNGC 4 cut(s) 40, 273, 822, 834
Sau3AI GATC 8 cut(s) 18, 174, 205, 339, 372, 493, 907, 1017
Sau96I GGNCC 5 cut(s) 69, 261, 594, 637, 922
ScrFI CCNGG 3 cut(s) 55, 406, 521
SduI GDGCHC 2 cut(s) 85, 553
SetI ASST 9 cut(s) 216, 324, 459, 537, 716, 838, 869, 924, 982
SfaNI GCATC 6 cut(s) 384, 427, 529, 550, 1018, 1049
SfcI CTRYAG 1 cut(s) 831
Sfr274I CTCGAG 2 cut(s) 78, 299
SinI GGWCC 2 cut(s) 637, 922
SlaI CTCGAG 2 cut(s) 78, 299
SmiMI CAYNNNNRTG 2 cut(s) 392, 786
SmlI CTYRAG 2 cut(s) 78, 299
SmoI CTYRAG 2 cut(s) 78, 299
Sse9I AATT 8 cut(s) 30, 154, 425, 500, 585, 624, 857, 1044
StyD4I CCNGG 3 cut(s) 53, 404, 519
StyI CCWWGG 2 cut(s) 144, 1050
TaaI ACNGT 5 cut(s) 383, 446, 475, 575, 622
TaiI ACGT 2 cut(s) 216, 324
TaqI TCGA 4 cut(s) 79, 123, 300, 1026
TasI AATT 8 cut(s) 30, 154, 425, 500, 585, 624, 857, 1044
TauI GCSGC 2 cut(s) 275, 824
TfiI GAWTC 3 cut(s) 24, 356, 1061
Tru1I TTAA 3 cut(s) 648, 963, 1081
Tru9I TTAA 3 cut(s) 648, 963, 1081
TscAI CASTG 2 cut(s) 478, 725
TseFI GTSAC 3 cut(s) 862, 890, 898
TseI GCWGC 2 cut(s) 39, 833
Tsp45I GTSAC 3 cut(s) 862, 890, 898
TspDTI ATGAA 4 cut(s) 621, 666, 822, 870
TspRI CASTG 2 cut(s) 478, 725
Tth111I GACNNNGTC 1 cut(s) 221
VpaK11BI GGWCC 2 cut(s) 637, 922
XapI RAATTY 3 cut(s) 425, 500, 857
XceI RCATGY 1 cut(s) 785
XhoI CTCGAG 2 cut(s) 78, 299
XmnI GAANNNNTTC 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.