Rroxscaffold_6G00392240

Oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0003907
Physical Location & Seq
Forward (+)
11693098 .. 11695149
2052 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00392240.1

Sequence Viewer

Length: 1095 bp
ATGTCTGAAAACCCAAAAATACGTTTCGGCATATTAGGATGTGCGAACATAGCTAGAAAGATAGCTCGCGCCATAAACCTGGCACCCAATTCGACCCTGTACGCCATTGCGAGCCGCTCCATCGAGAAAGCCCACAAATTCGCAGCCAACAATGGACTGTCCACTCAGACCCTCAAAATCTACGGCAGCTATGATCAGCTGCTCGATGACCCATGTGTCGATGCTGTGTACATGCCTCTGCCCACTAGTCTTCACCTTCACTGGGCTGTCTTGGCTGCCCGCAAGAAGAAGCACTTGCTCTTGGAAAAACCAGCGGCTCTTGATGTGGGTGAGCTTGATCAGATACTCCAAGCTTGCCAATCCAACGGTGTACAGTTCATGAGTGGGAGTATGTGGCTGCATCACCCTAGGACTGCCAAATTGAAGGACTTGATCTCTGATTCCAAGCTCTTTGGTCAAATCAACTACATTCATAGCTCATCAACACTTCCAGGAACCAAGGAATTTTTGGAGAACAACATAAGGGTGAAACCAGATTTAGATGCTCTCGGTGCGCTTGGGGACCTAGGCTGGTACTGCATTGAAGCCATTTTGTGGGCTAAGGGCTACCAACTACCAACTATAGTCACTGCTCTACCAGATGTCACAAAAAACTCTGCAGGGGTCATCTTGTCTTTCACTGCATCAATTCAATGGGAACCACTGGGCCCAACTGTTGCAACAATTCACTGTTCTTTCCTCTCTCACACATCCATGGACTTGGCTATATCTGCTTCTAACGGTACAGTGCGTTGCAATGACTACATCATTCCATATGCTGAGAACTATTCTTCATTTGAATTCACAACTGGTGCAAAGTTTGCGGAACTCCACATTGGGTGGAGTGTAGTACCTGAAGAAGTTAGAGTGGTTTCTCAGCTGCCTCAAGAGGCCTTGATGGTTCAAGAGCTCTCAAGGCTTGCCGAGGGCATCAGGGAATTTGGGTGTAATCCGGACCAAAAATGGCCACAAATCAGCAGAAATACACAATTAGTATTGGATGCAGTGAAGAAATCCATTGATCTTGGTTTTGAACCTGTTAGGGTGTTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GFO_IDH_MocA

Protein Analysis

364

Amino Acids

40.15

Weight (kDa)

6.55

Isoelectric Point (pI)

35.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GFO_IDH_MocA PF01408 7 - 129 1.3e-19 Oxidoreductase family, NAD-binding Rossmann fold
GFO_IDH_MocA_C3 PF22725 140 - 263 1.1e-06 GFO/IDH/MocA C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 215
AccB1I GGYRCC 1 cut(s) 82
AccB7I CCANNNNNTGG 1 cut(s) 594
AccBSI CCGCTC 1 cut(s) 117
AccII CGCG 1 cut(s) 69
AccIII TCCGGA 1 cut(s) 991
AciI CCGC 4 cut(s) 115, 280, 314, 863
AcoI YGGCCR 1 cut(s) 1004
AcsI RAATTY 4 cut(s) 137, 503, 839, 977
AcuI CTGAAG 1 cut(s) 915
AfaI GTAC 6 cut(s) 101, 230, 372, 575, 784, 891
AfiI CCNNNNNNNGG 2 cut(s) 262, 594
AgsI TTSAA 6 cut(s) 424, 584, 692, 839, 944, 1073
AhlI ACTAGT 1 cut(s) 245
AjnI CCWGG 2 cut(s) 78, 490
AjuI GAANNNNNNNTTGG 2 cut(s) 858, 890
Alw21I GWGCWC 1 cut(s) 951
Aor13HI TCCGGA 1 cut(s) 991
AoxI GGCC 3 cut(s) 706, 930, 1004
ApaI GGGCCC 1 cut(s) 710
ApeKI GCWGC 6 cut(s) 143, 186, 199, 275, 397, 919
ApoI RAATTY 4 cut(s) 137, 503, 839, 977
AspA2I CCTAGG 2 cut(s) 407, 565
AspLEI GCGC 2 cut(s) 71, 556
AspS9I GGNCC 4 cut(s) 562, 706, 707, 994
AsuHPI GGTGA 4 cut(s) 245, 341, 395, 538
AvaII GGWCC 2 cut(s) 562, 994
AvrII CCTAGG 2 cut(s) 407, 565
BaeGI GKGCMC 1 cut(s) 710
BalI TGGCCA 1 cut(s) 1006
BanI GGYRCC 1 cut(s) 82
BanII GRGCYC 2 cut(s) 710, 951
BbsI GAAGAC 1 cut(s) 242
Bbv12I GWGCWC 1 cut(s) 951
BbvI GCAGC 6 cut(s) 155, 186, 198, 262, 384, 906
BccI CCATC 2 cut(s) 128, 931
BceAI ACGGC 1 cut(s) 199
BcgI CGANNNNNNTGC 2 cut(s) 72, 106
BciT130I CCWGG 2 cut(s) 80, 492
BclI TGATCA 2 cut(s) 193, 337
BcuI ACTAGT 1 cut(s) 245
BfaI CTAG 4 cut(s) 54, 246, 408, 566
BfmI CTRYAG 2 cut(s) 621, 657
BisI GCNGC 8 cut(s) 115, 144, 187, 200, 276, 315, 398, 920
BlnI CCTAGG 2 cut(s) 407, 565
BlsI GCNGC 8 cut(s) 116, 145, 188, 201, 277, 316, 399, 921
Bme1390I CCNGG 2 cut(s) 80, 492
Bme18I GGWCC 2 cut(s) 562, 994
BmgT120I GGNCC 4 cut(s) 562, 706, 707, 994
BmiI GGNNCC 5 cut(s) 84, 496, 563, 699, 708
BmrFI CCNGG 2 cut(s) 80, 492
BmrI ACTGGG 2 cut(s) 271, 713
BmsI GCATC 6 cut(s) 211, 409, 532, 692, 978, 1030
BmuI ACTGGG 2 cut(s) 271, 713
BpiI GAAGAC 1 cut(s) 242
Bpu10I CCTNAGC 1 cut(s) 600
BpuEI CTTGAG 2 cut(s) 909, 937
BsaJI CCNNGG 5 cut(s) 407, 498, 565, 753, 963
BsaWI WCCGGW 1 cut(s) 991
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 594
Bse1I ACTGG 3 cut(s) 266, 708, 853
Bse3DI GCAATG 2 cut(s) 105, 802
BseAI TCCGGA 1 cut(s) 991
BseBI CCWGG 2 cut(s) 80, 492
BseDI CCNNGG 5 cut(s) 407, 498, 565, 753, 963
BseGI GGATG 3 cut(s) 44, 749, 1045
BseLI CCNNNNNNNGG 2 cut(s) 262, 594
BseMI GCAATG 2 cut(s) 105, 802
BseMII CTCAG 3 cut(s) 179, 810, 929
BseNI ACTGG 3 cut(s) 266, 708, 853
BseSI GKGCMC 1 cut(s) 710
BseXI GCAGC 6 cut(s) 155, 186, 198, 262, 384, 906
Bsh1236I CGCG 1 cut(s) 69
BshFI GGCC 3 cut(s) 708, 932, 1006
BshNI GGYRCC 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 951
BsiSI CCGG 1 cut(s) 992
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 2 cut(s) 262, 594
BsmFI GGGAC 1 cut(s) 575
BsnI GGCC 3 cut(s) 708, 932, 1006
Bsp120I GGGCCC 1 cut(s) 706
Bsp1286I GDGCHC 2 cut(s) 710, 951
Bsp13I TCCGGA 1 cut(s) 991
Bsp1407I TGTACA 2 cut(s) 228, 370
Bsp143I GATC 4 cut(s) 193, 337, 432, 1060
Bsp19I CCATGG 1 cut(s) 753
BspACI CCGC 4 cut(s) 115, 280, 314, 863
BspANI GGCC 3 cut(s) 708, 932, 1006
BspCNI CTCAG 3 cut(s) 178, 811, 928
BspEI TCCGGA 1 cut(s) 991
BspFNI CGCG 1 cut(s) 69
BspHI TCATGA 1 cut(s) 378
BspLI GGNNCC 5 cut(s) 84, 496, 563, 699, 708
BspMAI CTGCAG 1 cut(s) 661
BspT107I GGYRCC 1 cut(s) 82
BsrBI CCGCTC 1 cut(s) 117
BsrDI GCAATG 2 cut(s) 105, 802
BsrGI TGTACA 2 cut(s) 228, 370
BsrI ACTGG 3 cut(s) 266, 708, 853
BssECI CCNNGG 5 cut(s) 407, 498, 565, 753, 963
BssMI GATC 4 cut(s) 193, 337, 432, 1060
BssT1I CCWWGG 4 cut(s) 407, 498, 565, 753
Bst2UI CCWGG 2 cut(s) 80, 492
Bst4CI ACNGT 7 cut(s) 159, 368, 375, 715, 731, 782, 787
BstAPI GCANNNNNTGC 1 cut(s) 860
BstAUI TGTACA 2 cut(s) 228, 370
BstC8I GCNNGC 5 cut(s) 67, 112, 280, 355, 960
BstDEI CTNAG 4 cut(s) 165, 600, 819, 915
BstDSI CCRYGG 1 cut(s) 753
BstF5I GGATG 3 cut(s) 44, 749, 1045
BstFNI CGCG 1 cut(s) 69
BstHHI GCGC 2 cut(s) 71, 556
BstKTI GATC 4 cut(s) 196, 340, 435, 1063
BstMBI GATC 4 cut(s) 193, 337, 432, 1060
BstMWI GCNNNNNNNGC 7 cut(s) 50, 272, 551, 576, 770, 860, 955
BstNI CCWGG 2 cut(s) 80, 492
BstNSI RCATGY 1 cut(s) 235
BstSCI CCNGG 2 cut(s) 78, 490
BstSFI CTRYAG 2 cut(s) 621, 657
BstSLI GKGCMC 1 cut(s) 710
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 6 cut(s) 155, 186, 198, 262, 384, 906
BstV2I GAAGAC 1 cut(s) 242
BstXI CCANNNNNNTGG 2 cut(s) 79, 760
BsuRI GGCC 3 cut(s) 708, 932, 1006
BtgI CCRYGG 1 cut(s) 753
BtsCI GGATG 3 cut(s) 44, 749, 1045
BtsI GCAGTG 3 cut(s) 627, 678, 1050
BtsIMutI CAGTG 7 cut(s) 259, 627, 678, 701, 727, 792, 1050
Cac8I GCNNGC 5 cut(s) 67, 112, 280, 355, 960
CciI TCATGA 1 cut(s) 378
CfoI GCGC 2 cut(s) 71, 556
Cfr13I GGNCC 4 cut(s) 562, 706, 707, 994
Csp6I GTAC 6 cut(s) 100, 229, 371, 574, 783, 890
CviAII CATG 4 cut(s) 213, 232, 379, 754
CviQI GTAC 6 cut(s) 100, 229, 371, 574, 783, 890
DdeI CTNAG 4 cut(s) 165, 600, 819, 915
DpnI GATC 4 cut(s) 195, 339, 434, 1062
DpnII GATC 4 cut(s) 193, 337, 432, 1060
DrdI GACNNNNNNGTC 1 cut(s) 215
DseDI GACNNNNNNGTC 1 cut(s) 215
EaeI YGGCCR 1 cut(s) 1004
Ecl136II GAGCTC 1 cut(s) 949
Eco130I CCWWGG 4 cut(s) 407, 498, 565, 753
Eco147I AGGCCT 1 cut(s) 932
Eco24I GRGCYC 2 cut(s) 710, 951
Eco47I GGWCC 2 cut(s) 562, 994
Eco53kI GAGCTC 1 cut(s) 949
Eco57I CTGAAG 1 cut(s) 915
EcoICRI GAGCTC 1 cut(s) 949
EcoO109I RGGNCCY 1 cut(s) 562
EcoRI GAATTC 1 cut(s) 839
EcoRII CCWGG 2 cut(s) 78, 490
EcoT14I CCWWGG 4 cut(s) 407, 498, 565, 753
EcoT38I GRGCYC 2 cut(s) 710, 951
ErhI CCWWGG 4 cut(s) 407, 498, 565, 753
FaeI CATG 4 cut(s) 216, 235, 382, 757
FalI AAGNNNNNCTT 2 cut(s) 278, 310
FaqI GGGAC 1 cut(s) 575
FatI CATG 4 cut(s) 212, 231, 378, 753
FauI CCCGC 1 cut(s) 287
FauNDI CATATG 1 cut(s) 814
FbaI TGATCA 2 cut(s) 193, 337
Fnu4HI GCNGC 8 cut(s) 115, 144, 187, 200, 276, 315, 398, 920
FokI GGATG 3 cut(s) 51, 736, 1052
FriOI GRGCYC 2 cut(s) 710, 951
Fsp4HI GCNGC 8 cut(s) 115, 144, 187, 200, 276, 315, 398, 920
FspBI CTAG 4 cut(s) 54, 246, 408, 566
GlaI GCGC 2 cut(s) 70, 555
GluI GCNGC 8 cut(s) 115, 144, 187, 200, 276, 315, 398, 920
HaeIII GGCC 3 cut(s) 708, 932, 1006
HapII CCGG 1 cut(s) 992
HhaI GCGC 2 cut(s) 71, 556
Hin1II CATG 4 cut(s) 216, 235, 382, 757
Hin6I GCGC 2 cut(s) 69, 554
HinP1I GCGC 2 cut(s) 69, 554
HindIII AAGCTT 1 cut(s) 351
HinfI GANTC 1 cut(s) 440
HpaII CCGG 1 cut(s) 992
HphI GGTGA 4 cut(s) 245, 341, 395, 538
Hpy166II GTNNAC 3 cut(s) 162, 229, 371
Hpy188I TCNGA 4 cut(s) 7, 168, 342, 439
Hpy188III TCNNGA 6 cut(s) 124, 320, 379, 926, 944, 992
Hpy8I GTNNAC 3 cut(s) 162, 229, 371
HpyAV CCTTC 2 cut(s) 266, 418
HpyCH4III ACNGT 7 cut(s) 159, 368, 375, 715, 731, 782, 787
HpyCH4IV ACGT 1 cut(s) 22
HpyCH4V TGCA 8 cut(s) 400, 579, 659, 683, 719, 795, 854, 1043
HpyF10VI GCNNNNNNNGC 7 cut(s) 50, 272, 551, 576, 770, 860, 955
HpyF3I CTNAG 4 cut(s) 165, 600, 819, 915
HpySE526I ACGT 1 cut(s) 22
Hsp92II CATG 4 cut(s) 216, 235, 382, 757
HspAI GCGC 2 cut(s) 69, 554
Kpn2I TCCGGA 1 cut(s) 991
Ksp22I TGATCA 2 cut(s) 193, 337
Kzo9I GATC 4 cut(s) 193, 337, 432, 1060
LmnI GCTCC 1 cut(s) 122
Lsp1109I GCAGC 6 cut(s) 155, 186, 198, 262, 384, 906
LweI GCATC 6 cut(s) 211, 409, 532, 692, 978, 1030
MaeI CTAG 4 cut(s) 54, 246, 408, 566
MaeII ACGT 1 cut(s) 22
MaeIII GTNAC 2 cut(s) 625, 643
MalI GATC 4 cut(s) 195, 339, 434, 1062
MbiI CCGCTC 1 cut(s) 117
MboI GATC 4 cut(s) 193, 337, 432, 1060
MboII GAAGA 5 cut(s) 242, 298, 822, 908, 1060
MhlI GDGCHC 2 cut(s) 710, 951
MlsI TGGCCA 1 cut(s) 1006
MluCI AATT 9 cut(s) 88, 137, 419, 503, 687, 723, 839, 977, 1028
MluNI TGGCCA 1 cut(s) 1006
MmeI TCCRAC 1 cut(s) 387
MnlI CCTC 6 cut(s) 182, 246, 749, 922, 933, 958
Mox20I TGGCCA 1 cut(s) 1006
MroI TCCGGA 1 cut(s) 991
MscI TGGCCA 1 cut(s) 1006
MslI CAYNNNNRTG 2 cut(s) 524, 752
Msp20I TGGCCA 1 cut(s) 1006
MspA1I CMGCKG 3 cut(s) 199, 314, 919
MspI CCGG 1 cut(s) 992
MspR9I CCNGG 2 cut(s) 80, 492
MvaI CCWGG 2 cut(s) 80, 492
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 7 cut(s) 50, 272, 551, 576, 770, 860, 955
NcoI CCATGG 1 cut(s) 753
NdeI CATATG 1 cut(s) 814
NdeII GATC 4 cut(s) 193, 337, 432, 1060
NlaIII CATG 4 cut(s) 216, 235, 382, 757
NlaIV GGNNCC 5 cut(s) 84, 496, 563, 699, 708
NmeAIII GCCGAG 1 cut(s) 988
NmuCI GTSAC 2 cut(s) 625, 643
NspI RCATGY 1 cut(s) 235
PagI TCATGA 1 cut(s) 378
PceI AGGCCT 1 cut(s) 932
PfeI GAWTC 1 cut(s) 440
PflMI CCANNNNNTGG 1 cut(s) 594
PfoI TCCNGGA 1 cut(s) 490
PkrI GCNGC 8 cut(s) 116, 145, 188, 201, 277, 316, 399, 921
PpuMI RGGWCCY 1 cut(s) 562
Psp124BI GAGCTC 1 cut(s) 951
Psp5II RGGWCCY 1 cut(s) 562
Psp6I CCWGG 2 cut(s) 78, 490
PspGI CCWGG 2 cut(s) 78, 490
PspN4I GGNNCC 5 cut(s) 84, 496, 563, 699, 708
PspOMI GGGCCC 1 cut(s) 706
PspPI GGNCC 4 cut(s) 562, 706, 707, 994
PspPPI RGGWCCY 1 cut(s) 562
PstI CTGCAG 1 cut(s) 661
PvuII CAGCTG 2 cut(s) 199, 919
RsaI GTAC 6 cut(s) 101, 230, 372, 575, 784, 891
RsaNI GTAC 6 cut(s) 100, 229, 371, 574, 783, 890
RseI CAYNNNNRTG 2 cut(s) 524, 752
SacI GAGCTC 1 cut(s) 951
SatI GCNGC 8 cut(s) 115, 144, 187, 200, 276, 315, 398, 920
Sau3AI GATC 4 cut(s) 193, 337, 432, 1060
Sau96I GGNCC 4 cut(s) 562, 706, 707, 994
ScrFI CCNGG 2 cut(s) 80, 492
SduI GDGCHC 2 cut(s) 710, 951
SfaNI GCATC 6 cut(s) 211, 409, 532, 692, 978, 1030
SfcI CTRYAG 2 cut(s) 621, 657
SinI GGWCC 2 cut(s) 562, 994
SmiMI CAYNNNNRTG 2 cut(s) 524, 752
SmlI CTYRAG 2 cut(s) 924, 952
SmoI CTYRAG 2 cut(s) 924, 952
SpeI ACTAGT 1 cut(s) 245
Sse9I AATT 9 cut(s) 88, 137, 419, 503, 687, 723, 839, 977, 1028
SseBI AGGCCT 1 cut(s) 932
SsiI CCGC 4 cut(s) 115, 280, 314, 863
SspMI CTAG 4 cut(s) 54, 246, 408, 566
SstI GAGCTC 1 cut(s) 951
StuI AGGCCT 1 cut(s) 932
StyD4I CCNGG 2 cut(s) 78, 490
StyI CCWWGG 4 cut(s) 407, 498, 565, 753
TaaI ACNGT 7 cut(s) 159, 368, 375, 715, 731, 782, 787
TaiI ACGT 1 cut(s) 25
TaqI TCGA 4 cut(s) 92, 123, 204, 219
TasI AATT 9 cut(s) 88, 137, 419, 503, 687, 723, 839, 977, 1028
TatI WGTACW 2 cut(s) 228, 370
TauI GCSGC 2 cut(s) 117, 317
TfiI GAWTC 1 cut(s) 440
TscAI CASTG 7 cut(s) 266, 634, 685, 708, 734, 792, 1050
TseFI GTSAC 2 cut(s) 625, 643
TseI GCWGC 6 cut(s) 143, 186, 199, 275, 397, 919
Tsp45I GTSAC 2 cut(s) 625, 643
TspDTI ATGAA 3 cut(s) 367, 461, 822
TspRI CASTG 7 cut(s) 266, 634, 685, 708, 734, 792, 1050
Van91I CCANNNNNTGG 1 cut(s) 594
VpaK11BI GGWCC 2 cut(s) 562, 994
XapI RAATTY 4 cut(s) 137, 503, 839, 977
XceI RCATGY 1 cut(s) 235
XcmI CCANNNNNNNNNTGG 1 cut(s) 505
XmaJI CCTAGG 2 cut(s) 407, 565
XspI CTAG 4 cut(s) 54, 246, 408, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.