MD17G1262300.v1.1

Oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0003907
Physical Location & Seq
Forward (+)
32296884 .. 32299786
2903 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1262300.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGATGGCACTTCTAAAACAAAGGCAACAACCACCACCCTCATATCATCAGTCTCTCAACTCTCAGAGGTCAAAAATGGAAAAAAAAATGGCAGAAAATCCAGCAGCGATACGTTTTGGCATAACAGGATGCGCCGACATAGCCAGAAAAGTAGCTCGAGCCATAAAGCTCGCACCCAACGCCACCCTTTACGCTATCGCCAGCCGTTCGATCGACAAAGCCCGGAAATTCGCAGCCGACGGTGGACTCTCCGGAGCCGTCAAGATCTACGGGAGCTACGATCAGCTGCTCGACGATCCATGTGTAGATGCGGTGTACATGCCCCTGCCCACAAGTCTTCACCTGCACTGGGCGGTGTTGGCTGCTCGAAAGAAGAAGCATTTGCTTTTGGAAAAACCAACGGCTCTGGATGTGAGTGAGCTTGATCAGATACTGGAAGCGTGCGAATCCAACGGTGTACAGTTCATGGATGGCAGCATGTGGCTGCATCATCCCAGGACTGCCAAGTTGAAGGACTTGATCTCTGATCCCAAGCTTTTTGGTCAGATTGACATTATCCGTAGCACGTCAACAATGCCAGGAACCCAGGATTTCTTGGAGAGCAACATCAGAGTGAATCCGAACTTGGATGCTCTCGGTGCACTTGGGGACATAGGCTGGTACTGCATTGGGGCCATCTTGTGGGCAAAGGACTACAAATTACCAACTGTGGTGAATGCTCTACCTGATGTCCTCAAAAACTCTGCAGGGGTCATCTTGTCTTGCTCTGCATCAATTCATTGGGAACCCGCAGACAAAACCGTCGCCACAATTCACTTCTCTTTCCTCTCCCACTCGTCCATGGACTTGGCTATGACCGGTTCTAATGGTTCGATGCATTGCAACGACTACATAATTCCGTGCCAAGAGAATTCGGCCTCATTTGACTTTACATCCGGCGCAACATTTGTGGAACTCCATATCGGGTGGAATGCAAAACCAGGAGAAGTTACCGTCATTTCTGAGCTGCCGCAAGAGGCCTTGATGGTTCAAGAGTTTGCAAGGAATGCTGAGGACATTCGGAAATTTGGAAAGCGTCCGGACAGTAAATGGCCCGAGAGTAGCAGAAAAACGCAGTTAGTATTGGACGCGGTGAAGAAATCCATTGATCTTGGTTGTAAGCCTGTTAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GFO_IDH_MocA

Protein Analysis

392

Amino Acids

42.96

Weight (kDa)

8.04

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GFO_IDH_MocA PF01408 37 - 156 3.7e-18 Oxidoreductase family, NAD-binding Rossmann fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 351
AasI GACNNNNNNGTC 1 cut(s) 800
Acc36I ACCTGC 1 cut(s) 351
AccB7I CCANNNNNTGG 1 cut(s) 681
AccII CGCG 1 cut(s) 1130
AccIII TCCGGA 2 cut(s) 251, 1078
AciI CCGC 5 cut(s) 311, 353, 789, 1010, 1130
AclWI GGATC 2 cut(s) 290, 521
AcsI RAATTY 3 cut(s) 227, 910, 1064
AfaI GTAC 3 cut(s) 317, 459, 662
AfiI CCNNNNNNNGG 2 cut(s) 349, 681
AgeI ACCGGT 1 cut(s) 857
AgsI TTSAA 2 cut(s) 511, 1031
AjiI CACGTC 1 cut(s) 567
AjnI CCWGG 4 cut(s) 494, 577, 585, 979
AjuI GAANNNNNNNTTGG 2 cut(s) 608, 640
AluBI AGCT 7 cut(s) 155, 169, 276, 286, 421, 535, 1006
AluI AGCT 7 cut(s) 155, 169, 276, 286, 421, 535, 1006
Alw21I GWGCWC 1 cut(s) 643
Alw26I GTCTC 1 cut(s) 57
Alw44I GTGCAC 1 cut(s) 639
AlwI GGATC 2 cut(s) 290, 521
AlwNI CAGNNNCTG 1 cut(s) 433
Ama87I CYCGRG 2 cut(s) 156, 1094
Aor13HI TCCGGA 2 cut(s) 251, 1078
AoxI GGCC 4 cut(s) 672, 915, 1017, 1091
ApaLI GTGCAC 1 cut(s) 639
ApeKI GCWGC 7 cut(s) 104, 233, 286, 362, 474, 484, 1006
ApoI RAATTY 3 cut(s) 227, 910, 1064
AsiGI ACCGGT 1 cut(s) 857
AspLEI GCGC 2 cut(s) 134, 941
AspS9I GGNCC 2 cut(s) 672, 1092
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 3 cut(s) 332, 724, 1144
AvaI CYCGRG 2 cut(s) 156, 1094
BaeGI GKGCMC 1 cut(s) 643
BbsI GAAGAC 1 cut(s) 329
Bbv12I GWGCWC 1 cut(s) 643
BbvCI CCTCAGC 1 cut(s) 1050
BbvI GCAGC 7 cut(s) 116, 245, 273, 349, 471, 486, 993
BccI CCATC 3 cut(s) 464, 683, 1018
BceAI ACGGC 3 cut(s) 189, 242, 417
BciT130I CCWGG 4 cut(s) 496, 579, 587, 981
BclI TGATCA 1 cut(s) 424
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 1 cut(s) 57
BfmI CTRYAG 1 cut(s) 744
BfuAI ACCTGC 1 cut(s) 351
BglII AGATCT 1 cut(s) 264
BisI GCNGC 8 cut(s) 105, 234, 287, 363, 475, 485, 1007, 1010
BlsI GCNGC 8 cut(s) 106, 235, 288, 364, 476, 486, 1008, 1011
Bme1390I CCNGG 5 cut(s) 223, 496, 579, 587, 981
BmeT110I CYCGRG 2 cut(s) 156, 1094
BmgBI CACGTC 1 cut(s) 567
BmgT120I GGNCC 2 cut(s) 672, 1092
BmiI GGNNCC 4 cut(s) 256, 583, 673, 786
BmrFI CCNGG 5 cut(s) 223, 496, 579, 587, 981
BmrI ACTGGG 1 cut(s) 358
BmsI GCATC 6 cut(s) 119, 298, 496, 619, 779, 864
BmuI ACTGGG 1 cut(s) 358
BpiI GAAGAC 1 cut(s) 329
Bpu10I CCTNAGC 1 cut(s) 1050
BpuMI CCSGG 1 cut(s) 223
BsaJI CCNNGG 3 cut(s) 494, 585, 840
BsaWI WCCGGW 3 cut(s) 251, 857, 1078
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 681
Bse118I RCCGGY 1 cut(s) 857
Bse1I ACTGG 2 cut(s) 353, 438
Bse3DI GCAATG 1 cut(s) 877
BseAI TCCGGA 2 cut(s) 251, 1078
BseBI CCWGG 4 cut(s) 496, 579, 587, 981
BseDI CCNNGG 3 cut(s) 494, 585, 840
BseGI GGATG 6 cut(s) 134, 415, 475, 490, 634, 932
BseLI CCNNNNNNNGG 2 cut(s) 349, 681
BseMI GCAATG 1 cut(s) 877
BseMII CTCAG 3 cut(s) 77, 993, 1041
BseNI ACTGG 2 cut(s) 353, 438
BseSI GKGCMC 1 cut(s) 643
BseXI GCAGC 7 cut(s) 116, 245, 273, 349, 471, 486, 993
BsgI GTGCAG 1 cut(s) 329
Bsh1236I CGCG 1 cut(s) 1130
Bsh1285I CGRYCG 1 cut(s) 213
BshFI GGCC 4 cut(s) 674, 917, 1019, 1093
BshTI ACCGGT 1 cut(s) 857
BsiEI CGRYCG 1 cut(s) 213
BsiHKAI GWGCWC 1 cut(s) 643
BsiHKCI CYCGRG 2 cut(s) 156, 1094
BsiSI CCGG 5 cut(s) 223, 252, 858, 936, 1079
BslFI GGGAC 1 cut(s) 662
BslI CCNNNNNNNGG 2 cut(s) 349, 681
BsmAI GTCTC 1 cut(s) 57
BsmFI GGGAC 1 cut(s) 662
BsmI GAATGC 3 cut(s) 721, 976, 1051
BsnI GGCC 4 cut(s) 674, 917, 1019, 1093
BsoBI CYCGRG 2 cut(s) 156, 1094
Bsp1286I GDGCHC 1 cut(s) 643
Bsp13I TCCGGA 2 cut(s) 251, 1078
Bsp1407I TGTACA 2 cut(s) 315, 457
Bsp143I GATC 8 cut(s) 210, 264, 280, 295, 424, 519, 526, 1147
Bsp19I CCATGG 1 cut(s) 840
BspACI CCGC 5 cut(s) 311, 353, 789, 1010, 1130
BspANI GGCC 4 cut(s) 674, 917, 1019, 1093
BspCNI CTCAG 3 cut(s) 76, 994, 1042
BspEI TCCGGA 2 cut(s) 251, 1078
BspFNI CGCG 1 cut(s) 1130
BspLI GGNNCC 4 cut(s) 256, 583, 673, 786
BspMAI CTGCAG 1 cut(s) 748
BspMI ACCTGC 1 cut(s) 351
BspPI GGATC 2 cut(s) 290, 521
BsrDI GCAATG 1 cut(s) 877
BsrFI RCCGGY 1 cut(s) 857
BsrGI TGTACA 2 cut(s) 315, 457
BsrI ACTGG 2 cut(s) 353, 438
BssAI RCCGGY 1 cut(s) 857
BssECI CCNNGG 3 cut(s) 494, 585, 840
BssMI GATC 8 cut(s) 210, 264, 280, 295, 424, 519, 526, 1147
BssT1I CCWWGG 1 cut(s) 840
Bst2UI CCWGG 4 cut(s) 496, 579, 587, 981
Bst4CI ACNGT 7 cut(s) 242, 455, 462, 709, 802, 994, 1085
BstAPI GCANNNNNTGC 1 cut(s) 1046
BstAUI TGTACA 2 cut(s) 315, 457
BstC8I GCNNGC 3 cut(s) 171, 202, 442
BstDEI CTNAG 3 cut(s) 63, 1002, 1050
BstDSI CCRYGG 1 cut(s) 840
BstF5I GGATG 6 cut(s) 134, 415, 475, 490, 634, 932
BstFNI CGCG 1 cut(s) 1130
BstHHI GCGC 2 cut(s) 134, 941
BstKTI GATC 8 cut(s) 213, 267, 283, 298, 427, 522, 529, 1150
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 8 cut(s) 210, 264, 280, 295, 424, 519, 526, 1147
BstMCI CGRYCG 1 cut(s) 213
BstMWI GCNNNNNNNGC 6 cut(s) 140, 179, 359, 638, 663, 1046
BstNI CCWGG 4 cut(s) 496, 579, 587, 981
BstNSI RCATGY 2 cut(s) 322, 481
BstSCI CCNGG 5 cut(s) 221, 494, 577, 585, 979
BstSFI CTRYAG 1 cut(s) 744
BstSLI GKGCMC 1 cut(s) 643
BstUI CGCG 1 cut(s) 1130
BstV1I GCAGC 7 cut(s) 116, 245, 273, 349, 471, 486, 993
BstV2I GAAGAC 1 cut(s) 329
BstX2I RGATCY 1 cut(s) 264
BstXI CCANNNNNNTGG 1 cut(s) 847
BstYI RGATCY 1 cut(s) 264
BsuRI GGCC 4 cut(s) 674, 917, 1019, 1093
BtgI CCRYGG 1 cut(s) 840
BtrI CACGTC 1 cut(s) 567
BtsCI GGATG 6 cut(s) 134, 415, 475, 490, 634, 932
BtsIMutI CAGTG 1 cut(s) 346
BveI ACCTGC 1 cut(s) 351
Cac8I GCNNGC 3 cut(s) 171, 202, 442
CaiI CAGNNNCTG 1 cut(s) 433
CfoI GCGC 2 cut(s) 134, 941
Cfr10I RCCGGY 1 cut(s) 857
Cfr13I GGNCC 2 cut(s) 672, 1092
CseI GACGC 2 cut(s) 1064, 1136
Csp6I GTAC 3 cut(s) 316, 458, 661
CspAI ACCGGT 1 cut(s) 857
CspCI CAANNNNNGTGG 2 cut(s) 930, 965
CviAII CATG 5 cut(s) 300, 319, 466, 478, 841
CviQI GTAC 3 cut(s) 316, 458, 661
DdeI CTNAG 3 cut(s) 63, 1002, 1050
DpnI GATC 8 cut(s) 212, 266, 282, 297, 426, 521, 528, 1149
DpnII GATC 8 cut(s) 210, 264, 280, 295, 424, 519, 526, 1147
DrdI GACNNNNNNGTC 1 cut(s) 800
DseDI GACNNNNNNGTC 1 cut(s) 800
Eco130I CCWWGG 1 cut(s) 840
Eco147I AGGCCT 1 cut(s) 1019
Eco88I CYCGRG 2 cut(s) 156, 1094
EcoRI GAATTC 1 cut(s) 910
EcoRII CCWGG 4 cut(s) 494, 577, 585, 979
EcoT14I CCWWGG 1 cut(s) 840
EcoT22I ATGCAT 1 cut(s) 879
ErhI CCWWGG 1 cut(s) 840
FaeI CATG 5 cut(s) 303, 322, 469, 481, 844
FaqI GGGAC 1 cut(s) 662
FatI CATG 5 cut(s) 299, 318, 465, 477, 840
FauI CCCGC 1 cut(s) 796
FbaI TGATCA 1 cut(s) 424
Fnu4HI GCNGC 8 cut(s) 105, 234, 287, 363, 475, 485, 1007, 1010
FokI GGATG 6 cut(s) 141, 422, 477, 482, 641, 919
Fsp4HI GCNGC 8 cut(s) 105, 234, 287, 363, 475, 485, 1007, 1010
GlaI GCGC 2 cut(s) 133, 940
GluI GCNGC 8 cut(s) 105, 234, 287, 363, 475, 485, 1007, 1010
HaeIII GGCC 4 cut(s) 674, 917, 1019, 1093
HapII CCGG 5 cut(s) 223, 252, 858, 936, 1079
HgaI GACGC 2 cut(s) 1064, 1136
HhaI GCGC 2 cut(s) 134, 941
Hin1II CATG 5 cut(s) 303, 322, 469, 481, 844
Hin6I GCGC 2 cut(s) 132, 939
HinP1I GCGC 2 cut(s) 132, 939
HincII GTYRAC 1 cut(s) 570
HindII GTYRAC 1 cut(s) 570
HindIII AAGCTT 1 cut(s) 533
HinfI GANTC 3 cut(s) 246, 446, 616
HpaII CCGG 5 cut(s) 223, 252, 858, 936, 1079
HphI GGTGA 3 cut(s) 332, 724, 1144
Hpy166II GTNNAC 5 cut(s) 245, 316, 458, 570, 641
Hpy188I TCNGA 8 cut(s) 66, 429, 526, 546, 611, 621, 1003, 1062
Hpy188III TCNNGA 5 cut(s) 252, 262, 407, 1031, 1079
Hpy8I GTNNAC 5 cut(s) 245, 316, 458, 570, 641
Hpy99I CGWCG 3 cut(s) 242, 296, 806
HpyAV CCTTC 1 cut(s) 505
HpyCH4III ACNGT 7 cut(s) 242, 455, 462, 709, 802, 994, 1085
HpyCH4IV ACGT 2 cut(s) 112, 566
HpyF10VI GCNNNNNNNGC 6 cut(s) 140, 179, 359, 638, 663, 1046
HpyF3I CTNAG 3 cut(s) 63, 1002, 1050
HpySE526I ACGT 2 cut(s) 112, 566
Hsp92II CATG 5 cut(s) 303, 322, 469, 481, 844
HspAI GCGC 2 cut(s) 132, 939
Kpn2I TCCGGA 2 cut(s) 251, 1078
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 8 cut(s) 210, 264, 280, 295, 424, 519, 526, 1147
LmnI GCTCC 2 cut(s) 254, 273
Lsp1109I GCAGC 7 cut(s) 116, 245, 273, 349, 471, 486, 993
LweI GCATC 6 cut(s) 119, 298, 496, 619, 779, 864
MaeII ACGT 2 cut(s) 112, 566
MaeIII GTNAC 1 cut(s) 988
MalI GATC 8 cut(s) 212, 266, 282, 297, 426, 521, 528, 1149
MboI GATC 8 cut(s) 210, 264, 280, 295, 424, 519, 526, 1147
MboII GAAGA 3 cut(s) 329, 385, 1147
MflI RGATCY 1 cut(s) 264
MhlI GDGCHC 1 cut(s) 643
MluCI AATT 7 cut(s) 227, 698, 774, 810, 894, 910, 1064
MlyI GAGTC 1 cut(s) 240
MmeI TCCRAC 1 cut(s) 474
MnlI CCTC 7 cut(s) 49, 60, 743, 836, 928, 1009, 1045
Mph1103I ATGCAT 1 cut(s) 879
MroI TCCGGA 2 cut(s) 251, 1078
MseI TTAA 1 cut(s) 1167
MslI CAYNNNNRTG 1 cut(s) 611
MspA1I CMGCKG 1 cut(s) 286
MspI CCGG 5 cut(s) 223, 252, 858, 936, 1079
MspR9I CCNGG 5 cut(s) 223, 496, 579, 587, 981
Mva1269I GAATGC 3 cut(s) 721, 976, 1051
MvaI CCWGG 4 cut(s) 496, 579, 587, 981
MvnI CGCG 1 cut(s) 1130
MwoI GCNNNNNNNGC 6 cut(s) 140, 179, 359, 638, 663, 1046
NciI CCSGG 1 cut(s) 223
NcoI CCATGG 1 cut(s) 840
NdeII GATC 8 cut(s) 210, 264, 280, 295, 424, 519, 526, 1147
NlaIII CATG 5 cut(s) 303, 322, 469, 481, 844
NlaIV GGNNCC 4 cut(s) 256, 583, 673, 786
NsiI ATGCAT 1 cut(s) 879
NspI RCATGY 2 cut(s) 322, 481
PaeR7I CTCGAG 1 cut(s) 156
PaqCI CACCTGC 1 cut(s) 351
PceI AGGCCT 1 cut(s) 1019
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 3 cut(s) 721, 976, 1051
PfeI GAWTC 2 cut(s) 446, 616
PflMI CCANNNNNTGG 1 cut(s) 681
PinAI ACCGGT 1 cut(s) 857
PkrI GCNGC 8 cut(s) 106, 235, 288, 364, 476, 486, 1008, 1011
Ple19I CGATCG 1 cut(s) 213
PleI GAGTC 1 cut(s) 240
PpsI GAGTC 1 cut(s) 240
Psp6I CCWGG 4 cut(s) 494, 577, 585, 979
PspGI CCWGG 4 cut(s) 494, 577, 585, 979
PspN4I GGNNCC 4 cut(s) 256, 583, 673, 786
PspPI GGNCC 2 cut(s) 672, 1092
PspXI VCTCGAGB 1 cut(s) 156
PstI CTGCAG 1 cut(s) 748
PstNI CAGNNNCTG 1 cut(s) 433
PsuI RGATCY 1 cut(s) 264
PvuI CGATCG 1 cut(s) 213
PvuII CAGCTG 1 cut(s) 286
RsaI GTAC 3 cut(s) 317, 459, 662
RsaNI GTAC 3 cut(s) 316, 458, 661
RseI CAYNNNNRTG 1 cut(s) 611
SaqAI TTAA 1 cut(s) 1167
SatI GCNGC 8 cut(s) 105, 234, 287, 363, 475, 485, 1007, 1010
Sau3AI GATC 8 cut(s) 210, 264, 280, 295, 424, 519, 526, 1147
Sau96I GGNCC 2 cut(s) 672, 1092
SchI GAGTC 1 cut(s) 240
ScrFI CCNGG 5 cut(s) 223, 496, 579, 587, 981
SduI GDGCHC 1 cut(s) 643
SfaNI GCATC 6 cut(s) 119, 298, 496, 619, 779, 864
SfcI CTRYAG 1 cut(s) 744
Sfr274I CTCGAG 1 cut(s) 156
SlaI CTCGAG 1 cut(s) 156
SmiMI CAYNNNNRTG 1 cut(s) 611
SmlI CTYRAG 1 cut(s) 156
SmoI CTYRAG 1 cut(s) 156
Sse9I AATT 7 cut(s) 227, 698, 774, 810, 894, 910, 1064
SseBI AGGCCT 1 cut(s) 1019
SsiI CCGC 5 cut(s) 311, 353, 789, 1010, 1130
StuI AGGCCT 1 cut(s) 1019
StyD4I CCNGG 5 cut(s) 221, 494, 577, 585, 979
StyI CCWWGG 1 cut(s) 840
TaaI ACNGT 7 cut(s) 242, 455, 462, 709, 802, 994, 1085
TaiI ACGT 2 cut(s) 115, 569
TaqI TCGA 6 cut(s) 157, 209, 213, 291, 367, 872
TasI AATT 7 cut(s) 227, 698, 774, 810, 894, 910, 1064
TatI WGTACW 2 cut(s) 315, 457
TauI GCSGC 1 cut(s) 1012
TfiI GAWTC 2 cut(s) 446, 616
Tru1I TTAA 1 cut(s) 1167
Tru9I TTAA 1 cut(s) 1167
TscAI CASTG 1 cut(s) 353
TseI GCWGC 7 cut(s) 104, 233, 286, 362, 474, 484, 1006
TspDTI ATGAA 2 cut(s) 454, 767
TspGWI ACGGA 2 cut(s) 548, 888
TspRI CASTG 1 cut(s) 353
Van91I CCANNNNNTGG 1 cut(s) 681
VneI GTGCAC 1 cut(s) 639
XapI RAATTY 3 cut(s) 227, 910, 1064
XceI RCATGY 2 cut(s) 322, 481
XhoI CTCGAG 1 cut(s) 156
Zsp2I ATGCAT 1 cut(s) 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.