MD09G1270500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0010011
Physical Location & Seq
Forward (+)
34473621 .. 34474301
681 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1270500.v1.1.491

Sequence Viewer

Length: 681 bp
ATGCAGAGGAGAGAGCCGAGGGGAAGCTTCAACGACATCGATGAATTGGCAGTAGTAAAGGCAGCTGCATGGGCCTGGTTCGAGCGCGGTTCGTGTTCCGACAGCAAGTCGACCGTGCCGGAATTCGATTTAAGGAGGACTTGTCACGCTCCATATAAACCGTCCAGGTACAAGCTAGAAGCTATGGAAAATATAGCCAATATTACTAGTAAAGAAGGCACGGAATTTGTCAAGTTAGAATCTCCAAGCGTTACTACATCTAGTAACTCGCTTCTTGATACATATGAAATTGAAAGTATTTCTCGGAAATTGGATCAACTTATAGAGTTCAGTGGTGACAGAGACAAAGTTCACAAGGGTTTTCTAGTTGGAGATGTTCTTGGTACAAAGAACACGAAAGAGGGCGGGAGAGGTTCGTACTCTGATCTCGAATACAGAGATAAATCCTCTTTACCAGTGGAGCTACAAGTCCACGAAGACGTTCGTCAACAAGAAAGTTTGCCGCTCATGGATGGTAACGCGAATCGGAGGAAGAAAAAGAAGAACGTTAGAGGAGGGTTTTGGCATAGGCATGCGGCGATGTGTGGCAATAGTGAAGATGTTCTTAACACACAAGCTTTCGTGGTTAGTCGTCGGCGGCCAGAAAAGGGTGTATATGTACGTACCGGTACCGGTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

25.49

Weight (kDa)

8.9

Isoelectric Point (pI)

52.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 668
AccB1I GGYRCC 1 cut(s) 668
AccBSI CCGCTC 1 cut(s) 505
AccI GTMKAC 1 cut(s) 110
AccII CGCG 2 cut(s) 87, 521
AciI CCGC 5 cut(s) 87, 405, 503, 575, 637
AclI AACGTT 1 cut(s) 546
AclWI GGATC 1 cut(s) 321
AcoI YGGCCR 1 cut(s) 638
AcsI RAATTY 2 cut(s) 122, 224
AfaI GTAC 6 cut(s) 170, 385, 419, 660, 664, 670
AfiI CCNNNNNNNGG 1 cut(s) 647
AgeI ACCGGT 2 cut(s) 665, 671
AgsI TTSAA 2 cut(s) 31, 293
AhdI GACNNNNNGTC 1 cut(s) 106
AhlI ACTAGT 1 cut(s) 206
AjnI CCWGG 2 cut(s) 74, 164
AluBI AGCT 6 cut(s) 27, 65, 175, 182, 463, 617
AluI AGCT 6 cut(s) 27, 65, 175, 182, 463, 617
Alw26I GTCTC 1 cut(s) 336
AlwI GGATC 1 cut(s) 321
AoxI GGCC 2 cut(s) 72, 638
ApeKI GCWGC 2 cut(s) 62, 65
ApoI RAATTY 2 cut(s) 122, 224
AsiGI ACCGGT 2 cut(s) 665, 671
Asp700I GAANNNNTTC 2 cut(s) 480, 600
Asp718I GGTACC 1 cut(s) 668
AspLEI GCGC 1 cut(s) 87
AspS9I GGNCC 1 cut(s) 72
AsuHPI GGTGA 1 cut(s) 347
BanI GGYRCC 1 cut(s) 668
BbsI GAAGAC 1 cut(s) 483
BbvI GCAGC 2 cut(s) 52, 74
BccI CCATC 1 cut(s) 506
BciT130I CCWGG 2 cut(s) 76, 166
BcoDI GTCTC 1 cut(s) 336
BcuI ACTAGT 1 cut(s) 206
BfaI CTAG 4 cut(s) 176, 207, 261, 365
BisI GCNGC 5 cut(s) 63, 66, 503, 576, 638
BlsI GCNGC 5 cut(s) 64, 67, 504, 577, 639
Bme1390I CCNGG 2 cut(s) 76, 166
BmeRI GACNNNNNGTC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 1 cut(s) 670
BmrFI CCNGG 2 cut(s) 76, 166
BoxI GACNNNNGTC 1 cut(s) 483
BpiI GAAGAC 1 cut(s) 483
Bsa29I ATCGAT 1 cut(s) 39
BsaAI YACGTR 1 cut(s) 662
BsaJI CCNNGG 1 cut(s) 17
BsaWI WCCGGW 2 cut(s) 665, 671
BsaXI ACNNNNNCTCC 2 cut(s) 127, 157
Bsc4I CCNNNNNNNGG 1 cut(s) 647
Bse118I RCCGGY 2 cut(s) 665, 671
Bse1I ACTGG 1 cut(s) 455
BseBI CCWGG 2 cut(s) 76, 166
BseCI ATCGAT 1 cut(s) 39
BseDI CCNNGG 1 cut(s) 17
BseGI GGATG 1 cut(s) 517
BseLI CCNNNNNNNGG 1 cut(s) 647
BseNI ACTGG 1 cut(s) 455
BseRI GAGGAG 2 cut(s) 22, 567
BseXI GCAGC 2 cut(s) 52, 74
Bsh1236I CGCG 2 cut(s) 87, 521
Bsh1285I CGRYCG 1 cut(s) 114
BshFI GGCC 2 cut(s) 74, 640
BshNI GGYRCC 1 cut(s) 668
BshTI ACCGGT 2 cut(s) 665, 671
BshVI ATCGAT 1 cut(s) 39
BsiEI CGRYCG 1 cut(s) 114
BsiSI CCGG 3 cut(s) 119, 666, 672
BslI CCNNNNNNNGG 1 cut(s) 647
BsmAI GTCTC 1 cut(s) 336
BsnI GGCC 2 cut(s) 74, 640
Bsp143I GATC 2 cut(s) 313, 424
BspACI CCGC 5 cut(s) 87, 405, 503, 575, 637
BspANI GGCC 2 cut(s) 74, 640
BspDI ATCGAT 1 cut(s) 39
BspFNI CGCG 2 cut(s) 87, 521
BspLI GGNNCC 1 cut(s) 670
BspPI GGATC 1 cut(s) 321
BspT107I GGYRCC 1 cut(s) 668
BsrBI CCGCTC 1 cut(s) 505
BsrFI RCCGGY 2 cut(s) 665, 671
BsrI ACTGG 1 cut(s) 455
BssAI RCCGGY 2 cut(s) 665, 671
BssECI CCNNGG 1 cut(s) 17
BssMI GATC 2 cut(s) 313, 424
Bst2UI CCWGG 2 cut(s) 76, 166
Bst4CI ACNGT 2 cut(s) 115, 162
BstBAI YACGTR 1 cut(s) 662
BstC8I GCNNGC 1 cut(s) 573
BstF5I GGATG 1 cut(s) 517
BstFNI CGCG 2 cut(s) 87, 521
BstHHI GCGC 1 cut(s) 87
BstKTI GATC 2 cut(s) 316, 427
BstMAI GTCTC 1 cut(s) 336
BstMBI GATC 2 cut(s) 313, 424
BstMCI CGRYCG 1 cut(s) 114
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstNI CCWGG 2 cut(s) 76, 166
BstNSI RCATGY 1 cut(s) 575
BstPAI GACNNNNGTC 1 cut(s) 483
BstSCI CCNGG 2 cut(s) 74, 164
BstSNI TACGTA 1 cut(s) 662
BstUI CGCG 2 cut(s) 87, 521
BstV1I GCAGC 2 cut(s) 52, 74
BstV2I GAAGAC 1 cut(s) 483
Bsu15I ATCGAT 1 cut(s) 39
BsuRI GGCC 2 cut(s) 74, 640
BsuTUI ATCGAT 1 cut(s) 39
BtgZI GCGATG 1 cut(s) 593
BtsCI GGATG 1 cut(s) 517
BtsIMutI CAGTG 2 cut(s) 337, 462
Cac8I GCNNGC 1 cut(s) 573
CfoI GCGC 1 cut(s) 87
Cfr10I RCCGGY 2 cut(s) 665, 671
Cfr13I GGNCC 1 cut(s) 72
ClaI ATCGAT 1 cut(s) 39
Csp6I GTAC 6 cut(s) 169, 384, 418, 659, 663, 669
CspAI ACCGGT 2 cut(s) 665, 671
CviAII CATG 3 cut(s) 69, 508, 572
CviQI GTAC 6 cut(s) 169, 384, 418, 659, 663, 669
DpnI GATC 2 cut(s) 315, 426
DpnII GATC 2 cut(s) 313, 424
DriI GACNNNNNGTC 1 cut(s) 106
EaeI YGGCCR 1 cut(s) 638
Eam1105I GACNNNNNGTC 1 cut(s) 106
Eco105I TACGTA 1 cut(s) 662
EcoRI GAATTC 1 cut(s) 122
EcoRII CCWGG 2 cut(s) 74, 164
FaeI CATG 3 cut(s) 72, 511, 575
FalI AAGNNNNNCTT 4 cut(s) 124, 156, 588, 620
FatI CATG 3 cut(s) 68, 507, 571
FauI CCCGC 1 cut(s) 398
FauNDI CATATG 1 cut(s) 283
FblI GTMKAC 1 cut(s) 110
Fnu4HI GCNGC 5 cut(s) 63, 66, 503, 576, 638
FokI GGATG 1 cut(s) 524
Fsp4HI GCNGC 5 cut(s) 63, 66, 503, 576, 638
FspBI CTAG 4 cut(s) 176, 207, 261, 365
GlaI GCGC 1 cut(s) 86
GluI GCNGC 5 cut(s) 63, 66, 503, 576, 638
HaeIII GGCC 2 cut(s) 74, 640
HapII CCGG 3 cut(s) 119, 666, 672
HhaI GCGC 1 cut(s) 87
Hin1II CATG 3 cut(s) 72, 511, 575
Hin6I GCGC 1 cut(s) 85
HinP1I GCGC 1 cut(s) 85
HincII GTYRAC 2 cut(s) 111, 488
HindII GTYRAC 2 cut(s) 111, 488
HindIII AAGCTT 2 cut(s) 25, 615
HinfI GANTC 2 cut(s) 239, 523
HpaII CCGG 3 cut(s) 119, 666, 672
HphI GGTGA 1 cut(s) 347
Hpy166II GTNNAC 4 cut(s) 111, 352, 472, 488
Hpy188I TCNGA 4 cut(s) 100, 306, 424, 528
Hpy188III TCNNGA 2 cut(s) 275, 428
Hpy8I GTNNAC 4 cut(s) 111, 352, 472, 488
Hpy99I CGWCG 1 cut(s) 636
HpyAV CCTTC 1 cut(s) 209
HpyCH4III ACNGT 2 cut(s) 115, 162
HpyCH4IV ACGT 3 cut(s) 480, 546, 661
HpyCH4V TGCA 2 cut(s) 4, 68
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpySE526I ACGT 3 cut(s) 480, 546, 661
Hsp92II CATG 3 cut(s) 72, 511, 575
HspAI GCGC 1 cut(s) 85
KpnI GGTACC 1 cut(s) 672
Kzo9I GATC 2 cut(s) 313, 424
LmnI GCTCC 2 cut(s) 154, 460
LpnPI CCDG 7 cut(s) 61, 88, 132, 151, 178, 468, 654
Lsp1109I GCAGC 2 cut(s) 52, 74
MaeI CTAG 4 cut(s) 176, 207, 261, 365
MaeII ACGT 3 cut(s) 480, 546, 661
MaeIII GTNAC 5 cut(s) 143, 250, 263, 335, 515
MalI GATC 2 cut(s) 315, 426
MbiI CCGCTC 1 cut(s) 505
MboI GATC 2 cut(s) 313, 424
MboII GAAGA 4 cut(s) 488, 544, 553, 608
MluCI AATT 5 cut(s) 44, 122, 224, 288, 308
MmeI TCCRAC 2 cut(s) 123, 349
MnlI CCTC 8 cut(s) 12, 129, 394, 404, 457, 522, 545, 548
MroXI GAANNNNTTC 2 cut(s) 480, 600
MseI TTAA 3 cut(s) 131, 606, 679
MslI CAYNNNNRTG 1 cut(s) 570
MspA1I CMGCKG 1 cut(s) 65
MspI CCGG 3 cut(s) 119, 666, 672
MspR9I CCNGG 2 cut(s) 76, 166
MvaI CCWGG 2 cut(s) 76, 166
MvnI CGCG 2 cut(s) 87, 521
MwoI GCNNNNNNNGC 1 cut(s) 71
NdeI CATATG 1 cut(s) 283
NdeII GATC 2 cut(s) 313, 424
NlaIII CATG 3 cut(s) 72, 511, 575
NlaIV GGNNCC 1 cut(s) 670
NmeAIII GCCGAG 1 cut(s) 42
NmuCI GTSAC 2 cut(s) 143, 335
NspI RCATGY 1 cut(s) 575
PaeI GCATGC 1 cut(s) 575
PdmI GAANNNNTTC 2 cut(s) 480, 600
PfeI GAWTC 2 cut(s) 239, 523
PinAI ACCGGT 2 cut(s) 665, 671
PkrI GCNGC 5 cut(s) 64, 67, 504, 577, 639
Ppu21I YACGTR 1 cut(s) 662
PshAI GACNNNNGTC 1 cut(s) 483
Psp1406I AACGTT 1 cut(s) 546
Psp6I CCWGG 2 cut(s) 74, 164
PspGI CCWGG 2 cut(s) 74, 164
PspN4I GGNNCC 1 cut(s) 670
PspPI GGNCC 1 cut(s) 72
PvuII CAGCTG 1 cut(s) 65
RsaI GTAC 6 cut(s) 170, 385, 419, 660, 664, 670
RsaNI GTAC 6 cut(s) 169, 384, 418, 659, 663, 669
RseI CAYNNNNRTG 1 cut(s) 570
SalI GTCGAC 1 cut(s) 109
SaqAI TTAA 3 cut(s) 131, 606, 679
SatI GCNGC 5 cut(s) 63, 66, 503, 576, 638
Sau3AI GATC 2 cut(s) 313, 424
Sau96I GGNCC 1 cut(s) 72
ScrFI CCNGG 2 cut(s) 76, 166
SmiMI CAYNNNNRTG 1 cut(s) 570
SnaBI TACGTA 1 cut(s) 662
SpeI ACTAGT 1 cut(s) 206
SphI GCATGC 1 cut(s) 575
Sse9I AATT 5 cut(s) 44, 122, 224, 288, 308
SsiI CCGC 5 cut(s) 87, 405, 503, 575, 637
SspI AATATT 1 cut(s) 202
SspMI CTAG 4 cut(s) 176, 207, 261, 365
StyD4I CCNGG 2 cut(s) 74, 164
TaaI ACNGT 2 cut(s) 115, 162
TaiI ACGT 3 cut(s) 483, 549, 664
TaqI TCGA 5 cut(s) 39, 81, 110, 126, 429
TasI AATT 5 cut(s) 44, 122, 224, 288, 308
TauI GCSGC 3 cut(s) 505, 578, 640
TfiI GAWTC 2 cut(s) 239, 523
Tru1I TTAA 3 cut(s) 131, 606, 679
Tru9I TTAA 3 cut(s) 131, 606, 679
TscAI CASTG 2 cut(s) 337, 462
TseFI GTSAC 2 cut(s) 143, 335
TseI GCWGC 2 cut(s) 62, 65
Tsp45I GTSAC 2 cut(s) 143, 335
TspDTI ATGAA 2 cut(s) 57, 300
TspGWI ACGGA 1 cut(s) 236
TspRI CASTG 2 cut(s) 337, 462
XapI RAATTY 2 cut(s) 122, 224
XceI RCATGY 1 cut(s) 575
XmiI GTMKAC 1 cut(s) 110
XmnI GAANNNNTTC 2 cut(s) 480, 600
XspI CTAG 4 cut(s) 176, 207, 261, 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.